ライフサイエンスコーパス: gap junction channel

1 at contribute to the ion selectivity of this gap junction channel.

2 irect binding of the drug to the pore of the gap junction channel.

3 hes plays a critical role in the assembly of gap junction channels.

4 up fluorescent tracer molecules permeant to gap junction channels.

5 use (19), revealing significant diversity in gap junction channels.

6 ty ratios were unchanged from wild-type Cx40 gap junction channels.

7 th that generated by IP(3) diffusing through gap junction channels.

8 uted to a direct effect on the properties of gap junction channels.

9 r partners, thus modifying the regulation of gap junction channels.

10 connexin selectivity, or gating polarity of gap junction channels.

11 modulated by the connexin composition of the gap junction channels.

12 directly on, or intimately associated with, gap junction channels.

13 ustering may be a requirement for opening of gap junction channels.

14 pears to be unrelated to its role in forming gap junction channels.

15 ing of LR determinants through intercellular gap junction channels.

16 gre proteins may join in forming heteromeric gap junction channels.

17 gene v-src appears to cause acute closure of gap junction channels.

18 ce states are a commonly observed feature of gap junction channels.

19 astrocytes by direct signaling via homotypic gap junction channels.

20 cortex are coupled together into clusters by gap junction channels.

21 ake exhibited properties similar to those of gap junction channels.

22 etabolite exchange, as well as precursors to gap junction channels.

23 ng B-cells that are electrically coupled via gap junction channels.

24 networks to support information transfer via gap junction channels.

25 ins partake in proposed gating mechanisms of gap junction channels.

26 of the astrocyte-to-astrocyte couplings via gap junction channels.

27 g cells, a process thought to be mediated by gap junction channels.

28 : Connexins are the pore forming subunits of gap junction channels.

29 d stability, and unitary conductance of Cx50 gap junction channels.

30 le mutants of Ala-40 did not form functional gap junction channels.

31 Connexin proteins are the subunits of gap junction channels.

32 st in part, by fast (in minutes) turnover of gap junction channels.

33 by two adjacent hemichannels, which can form gap junction channels.

34 llular electrical signaling independent from gap junction channels.

35 nnexins is an important mechanism regulating gap junction channels.

36 (Cx43) is a transmembrane protein that forms gap junction channels.

37 ercellular communication that occurs through gap junction channels.

38 r to connexins, but do not form cell-to-cell gap junction channels.

39 requires the expression of connexin43 (Cx43) gap junction channels.

40 ns during embryogenesis, can form oligomeric gap-junction channels.

41 Aberrant cell-cell coupling through gap junction channels, a process termed gap junction rem

42 namics of intercellular dye transfer through gap junction channels, a technique we named Trojan-local

43 Strikingly, the gap junction channel activity of UNC-9 is dispensable fo

44 paradigm that ERKs only negatively regulate gap junction channel activity.

45 Gap junction channels allow for the passage of ions and

46 n among intercellular adhesion molecules and gap junction channels along arteriolar endothelium.

47 ests the spread of hyperpolarization through gap junction channels along endothelium.

48 ell communication through connexin 43 (Cx43) gap junction channels also plays a major role in the wou

49 odels for a symmetrical (homotypic) connexin gap junction channel and were described by either a one-

50 rived lipid vesicles that contain functional gap junction channels and encapsulate molecular cargos.

51 Gap junction channels and hemichannels are permeable to

52 evel of connexin43 (Cx43), a protein forming gap junction channels and hemichannels associated with d

53 ichannels is important to assess the role of gap junction channels and hemichannels in health and dis

54 tly modifies the gating of connexin26 (Cx26) gap junction channels and hemichannels.

55 mily of membrane-spanning proteins that form gap junction channels and hemichannels.

56 of connexins, the molecular constituents of gap junction channels and hemichannels.

57 Electrophysiological properties of gap junction channels and mechanisms involved in the pro

58 otein interactions in the regulation of Cx32 gap junction channels and myelin homeostasis.

59 duces intercellular communication by closing gap junction channels and subsequently internalizing gap

60 ceptor" model for pH gating of Cx40 and Cx43 gap junction channels and suggest that interactions betw

61 Here, we discuss the basic structure of gap junction channels and the function of connexin genes

62 ies targeted at elucidating the mechanism of gap junction channels and the molecular basis of disease

63 , no information exists on the prevalence of gap junction channels and their function in the aging br

64 s, that have permeability similar to that of gap junction channels and thus can be utilized in studie

65 gomerize with each other to form heteromeric gap junction channels and to determine the functional an

66 miR-499 traverses gap junction channels and translocates to structurally c

67 ial action of CO(2) on Cx26 hemichannels and gap junction channels, and increase support for the role

68 ons, and forms puncta before the clusters of gap junction channels appear on the membrane.

69 KEY POINTS: Gap junction channels are essential for the formation an

70 We investigated whether gap junction channels are formed between endothelial cel

71 Vertebrate gap junction channels are formed by a family of more tha

72 Gap junction channels are formed by integral membrane pr

73 Gap junction channels are formed by members of the conne

74 Gap junction channels are formed by paired oligomeric me

75 Gap junction channels are formed by two unrelated protei

76 Gap junction channels are intercellular channels that me

77 unds antagonize the inhibition suggests that gap junction channels are regulated by a complex interpl

78 Gap junction channels are required for normal cardiac im

79 dependence and single-channel conductance of gap junction channels as well as for studies of chemical

80 calmodulin-binding sites, and their role in gap junction channel assembly was investigated.

81 embranes to study the mechanisms involved in gap junction channel assembly.

82 Our data suggest that the selectivity of gap junction channels at intercalated disks is increased

83 Conductance of gap junction channels at large myelinated club endings i

84 A (Hela/Cx46) assembled Cx46 into functional gap junction channels at the cell surface.

85 HCs) are hexamers of connexins that can form gap-junction channels at points of cell contacts or "fre

86 member of the family of proteins that forms gap junction channels between cells, was immunoaffinity-

87 l gating characteristics of connexin (Cx) 50 gap junction channels between pairs of N2A neuroblastoma

88 Addition of carbenoxolone (200 microM), a gap junction channel blocker, to the media stopped spont

89 Moreover, our results showing that gap junction channel blockers (heptanol, octanol, carben

90 and dental pain and if pannexin or pannexin/gap junction channel blockers reduce stimulation-depende

91 re inhibited by three structurally different gap-junction channel blockers, but not by the P2X(7) blo

92 The R75W phenotype is dominant at the gap-junction channel but not at the hemichannel level.

93 Inhibition of gap junction channels by FFA (pKa approximately 3.8) was

94 x36, suggesting that the open probability of gap junction channels can approach 100% under certain co

95 a8 connexin (Cx50 or Gja8), subunits of lens gap junction channels, cause a variety of cataracts via

96 gests that the path of communication through gap junction channels circumvents the node and streak.

97 mutation Lys108Arg prevents CO(2)-dependent gap junction channel closure in human Cx26.

98 lysine 125 is essential for CO(2)-dependent gap junction channel closure.

99 Gap junction channels communicate the cytoplasms of two

100 ave identified the pore-lining residues of a gap junction channel composed of Cx32.

101 increases the chemical gating sensitivity of gap junction channels composed of connexin 32 and decrea

102 Gap junction channels composed of connexin43 (Cx43) are

103 Thus, gap junction channels comprised of connexin 43 and other

104 fness in humans, which are knockouts for the gap-junction channels connexin 26 and connexin 30 genes,

105 ness in humans, which are knock-outs for the gap-junction channels connexin 26 and connexin 30 genes,

106 A gap junction channel consists of two connexons, one from

107 electrical synapses, composed of plaques of gap junction channels, continuously transmit signals tha

108 S-nitrosylation of Cx43 gap junction channels critically regulates communication

109 FFA reduced gap junction channel currents in a reversible and concen

110 enamic acid (FFA) and related derivatives on gap junction channel currents, applying the dual whole-c

111 results suggest that in addition to forming gap junction channels, Cx50 acts as an adhesive molecule

112 The authors conclude that the roles of lens gap junction channels depend not only on the primary seq

113 hat chemical regulation of connexin43 (Cx43) gap junction channels depends on the integrity of the ca

114 e-channel conductance of the hCx37 homotypic gap junction channel does not saturate with transjunctio

115 pse is based on electrical properties of the gap junction channel encompassing two fast and two slow

116 rmacological blockade or genetic knockout of gap junction channels expressed by ipRGCs, which reduces

117 mice showed marked perturbations of cardiac gap junction channel expression and localization, includ

118 we designed a murine model of heterogeneous gap junction channel expression.

119 (Cx32) can oligomerize to form intracellular gap junction channels facilitating a shorter pathway for

120 monstrated junctional currents indicative of gap junction channel formation.

121 Gap junction channels formed by alpha3 (Cx46) and alpha8

122 ontrast, quinine did not substantially block gap junction channels formed by Cx26, Cx32, Cx40, and Cx

123 from cortical fiber cells to the nucleus via gap junction channels formed by Cx46.

124 Gap junction channels formed by most connexins are affec

125 The gap junction channels formed in the A7r5 cells display m

126 y-associated gene Neurobeachin, the neuronal gap junction channel-forming Connexins, and the electric

127 Connexins are gap junction channel-forming protein subunits.

128 y of critical importance because loss of the gap junction channel-forming proteins, connexins Cx32 an

129 Mutations in Cx32, a gap-junction channel-forming protein, result in X-linked

130 eptides as regulators of Cx43 expression and gap junction channel function in dissociated myocytes an

131 vity of connexin proteins was independent of gap junction channel function, because physical isolatio

132 of a region of Cx43CT that is fundamental to gap junction channel function.

133 kin-Huxley equations and a 36-state model of gap junction channel gating to simulate electrical signa

134 Cytosolic changes control gap junction channel gating via poorly understood mechan

135 reduces junctional conductance by modifying gap junction channel gating, while maintaining cells in

136 protein (Cx43-EGFP) to examine mechanisms of gap junction channel gating.

137 ssociated with germline inactivation of this gap junction channel gene and uncovered an essential rol

138 d characterization of smedinx-11--an innexin gap junction channel gene expressed in the adult stem ce

139 me due to mutations in the connexin43 (Cx43) gap junction channel gene.

140 ons and the consequences on hemichannels and gap junction channel (GJC) functions remain unknown.

141 allow intercellular communication by forming gap junction channels (GJCs) between juxtaposed cells.

142 Gap junction channels (GJCs) mediate intercellular commu

143 amily of vertebrate proteins constituents of gap junction channels (GJCs) that connect the cytoplasm

144 by forming functional hemichannels (HCs) and gap junction channels (GJCs), respectively.

145 detail, the modulation of ionic transport in gap-junction channels (GJCs).

146 The physiological function of gap junction channels goes well beyond their initially d

147 contrast, anandamide, a blocker of connexin gap junction channels, had no effect of cell-to-cell com

148 The gap junction channels have a low and high conductance st

149 only a few defined molecules passing through gap junction channels have been linked to specific funct

150 gating characteristics of human Cx43-derived gap junction channels have been used.

151 The model assumes that the conductive gap junction channels have four conformational states.

152 Intercellular connexin channels (gap junction channels) have long been thought to mediate

153 mbrane and forms functional hemichannels and gap junction channels; however, it causes cell death eve

154 effects of VVAA mutants on hemichannels and gap junction channels imply that inter-TM interactions c

155 own that a stomatin-like protein regulates a gap junction channel in Caenorhabditis elegans.

156 In WT lenses, all gap junction channels in DFs close when pH is reduced, w

157 en adenosine receptors and the regulation of gap junction channels in endothelial cells of the blood-

158 s a multicellular system and for the role of gap junction channels in exacerbating the effects of dec

159 but electrophysiological characterization of gap junction channels in freshly isolated vascular smoot

160 g rates of intercellular dye transfer across gap junction channels in intact living cells.

161 unidirectionally throughout the epiblast by gap junction channels in order to pattern left-sided Shh

162 This protein, in addition to forming gap junction channels in paired oocytes, can also form a

163 (Cx26) and Cx30 are predominant isoforms of gap junction channels in the cochlea and play a critical

164 ible for acidification-induced uncoupling of gap junction channels in the heart and in other Cx43-exp

165 Loss of connexin43 (Cx43) gap junction channels in the heart results in a marked i

166 NSY-5 forms hemichannels and intercellular gap-junction channels in Xenopus oocytes, consistent wit

167 the phosphorylation state can regulate Cx32 gap junction channels, in addition to the direct interac

168 ally useful method to block certain types of gap junction channels, including those between neurons t

169 The rat connexin40 gap junction channel is permeable to monovalent cations

170 cess in which intercellular coupling through gap junction channels is a critical component.

171 Intercellular coupling via connexin40 (Cx40) gap junction channels is an important determinant of imp

172 7 not directly related to forming functional gap junction channels is not known.

173 egarding the regulation of connexin43 (Cx43) gap junction channels is that, upon intracellular acidif

174 Chicken (Gallus gallus) Cx26 gap junction channels lack Lys108 and do not close to CO

175 fferent combinations of Cx46 and Cx50 within gap junction channels lead to unique biophysical propert

176 Gap junction channels made of different connexins have d

177 oposed that intercellular signalling through gap junction channels may influence aspects of follicula

178 oleamide- induced inactivation of glial cell gap junction channels may serve to regulate communicatio

179 As gap-junction channels may be formed by both homotypic an

180 Gap junction channels mediate intercellular signalling t

181 hether intercellular coupling via connexin43 gap junction channels modulates hormonal responsiveness

182 d the unitary conductance of hCx37 homotypic gap junction channels more than predicted by screening a

183 We isolated gap junction channel mutants that reduce coupling betwee

184 We show that key determinants Cx46 and Cx50 gap junction channel open stability and unitary conducta

185 of junctional resistance (or conductance) of gap junction channels or connexin hemichannels in the du

186 Both failed to form gap junction channels or hemichannels when expressed alo

187 second messengers, including Ca(2+), through gap junction channels, or by a paracrine pathway that in

188 ied in the patient displayed no formation of gap junction channel plaques.

189 ssion of Cx26-Asp50Asn and wild-type Cx26 in gap junction channel plaques.

190 Gap junction channels play an important role in cell gro

191 e the major constituent proteins of cochlear gap junction channels, possibly in a unique heteromeric

192 s appears to interfere with formation of new gap junction channels, presumably by reducing insertion

193 ferent protein families, including the human gap junction channel protein connexin 26, the ATP bindin

194 Loss of the GJA1-encoded gap junction channel protein connexin43 is known to unde

195 a null allele in the gene encoding the major gap junction channel protein, connexin43 (Cx43).

196 d the unitary conductance of this Cx40E9,13K gap junction channel protein.

197 e-specific patterns of expression of diverse gap junction channel proteins (connexins), and regulator

198 cytes can rapidly adjust their expression of gap junction channel proteins in response to changes in

199 Atrial myocytes express three different gap junction channel proteins-connexin43 (Cx43), connexi

200 Gap junction channels providing direct cell-cell communi

201 endently inhibit Cx26-Asp50Asn expression in gap junction channels, reverting the dominant negative e

202 Electrical coupling via gap junction channels shapes the light response properti

203 Single Cx50D3E gap junction channels showed reduced unitary conductance

204 tran (to 0.22) and heptanol, an uncoupler of gap junction channels, significantly decreased it (to 0.

205 Gap junction channels span the membranes of two adjacent

206 Several different gap junction channel subunit isotypes, known as connexin

207 It forms gap junction channels that act as electrical synapses.

208 e integral membrane proteins forming aqueous gap junction channels that allow the diffusional exchang

209 Connexins (Cx) are protein components of gap junction channels that permit the passage of small m

210 omeric, there exists a multitude of possible gap-junction channels that could underlie the homotypic

211 Contrary to previous conceptions of the gap junction channel, the residues lining the pore are l

212 Interestingly, in addition to gap junction channels, the most abundant connexin (Cx) i

213 ouring progenitors through connexin43 (Cx43) gap junction channels, thereby coordinating the migratio

214 the cytosol to the extracellular space or as gap junction channels to provide a pathway for solute ex

215 Thus, the data indicate that halothane gates gap junction channels to the closed state in a dose-depe

216 f large-pore channels starting from connexin gap junction channels to the more recent developments in

217 ata provide additional evidence for multiple gap junction channel types in the human epidermis.

218 The structure of a recombinant cardiac gap junction channel was determined by electron crystall

219 d rats showed that the density of fiber cell gap junction channels was approximately the same, intrac

220 Direct action of at-RA on gap junction channels was further supported by its equiv

221 g current observed for Cx26/Cx32 heterotypic gap junction channels were determined in transfected mou

222 In human airway cells, gap junction channels were found to provide a regulated

223 de a maximum conductance of ~1200 pS, if all gap junction channels were open.

224 sticated cell-cell communication network via gap junction channels, which are made up of at least thr

225 electrically coupled via connexin-36 (Cx36) gap junction channels, which coordinates the pulsatile d

226 e X-ray crystal structures of the human Cx26 gap junction channel with and without bound Ca(2+).

227 extent to which malignant glioma cells form gap junction channels with astrocytes from either adult

228 Here we show that these vesicles form gap junction channels with cells, opening a direct and e

229 clamp revealed that endothelial cells formed gap junction channels with Cx43+/+ but not Cx43-/- proge

230 pressing glioma cells established functional gap junction channels with host astrocytes and dispersed

231 further demonstrated that blocking pannexin/gap junction channels with probenecid or carbenoxolone s

232 ge as second messenger molecules endows Cx36 gap junction channels with properties well suited for me

233 olar wall form connexin 43 (Cx43)-containing gap junction channels with the epithelium.

234 43-A44V and Cx43-E227D all formed functional gap junction channels with the same efficiency as wild-t

235 olecule being sufficient to occlude the Cx40 gap junction channel within the KCl permeation pathway.

236 43 showed a transdominant inhibition of Cx43 gap junction channels, without reductions in Cx43 protei