ライフサイエンスコーパス: gating current

1 ht beta 1A coexpressing cells had measurable gating currents).

2 bsence of conductance and drastic changes in gating current.

3 hich leads to a "Boltzmann equation" for the gating current.

4 rged residues, and this movement generates a gating current.

5 process and thus the physical nature of the gating current.

6 ctivation process and thus the nature of the gating current.

7 reversal of toxin action and toxin block of gating current.

8 on by an applied electric field produces the gating current.

9 ctivation while having negligible effects on gating currents.

10 ates off-gating currents without altering on-gating currents.

11 mately -40 mV as expected for sodium channel gating currents.

12 d by previous work involving measurements of gating currents.

13 imentally with large-bandwidth recordings of gating currents.

14 from the VSD prevented the mode-shift of the gating currents.

15 ts of voltage sensor movement in the form of gating currents.

16 ed mammalian cells, they both express robust gating currents.

17 vement of the voltage sensor, as measured by gating currents.

18 -dependence of the drug with macroscopic and gating currents.

19 and voltage dependence of ionic currents and gating currents.

20 and fast inactivation, we characterized HERG gating currents.

21 the effects of internal Ca(2+) on BK channel gating currents.

22 , the largest Q-V shift, and the fastest OFF gating currents.

23 ltage-clamped for measurements of Ca(2+) and gating currents.

24 model reproduces the signature properties of gating current: 1) equality of ON and OFF charge Q in in

25 ality of ON and OFF charge Q in integrals of gating current, 2) saturating voltage dependence in the

26 ocal anaesthetic drugs to modify Na+ channel gating currents, a Cys was substituted for the Phe in th

27 nit (only four out of eleven cells exhibited gating currents above the limits of detection, whereas e

28 to simulate the time dependence of the fast gating current allows us to reproduce the observed trend

29 Using voltage-clamp fluorimetry and gating current analysis, we demonstrate that proline sub

30 ubstantiated by measurements of Ca2+ channel gating currents and by analysis of another channel mutat

31 Measurement of gating currents and concentration-dependent availability

32 d in the voltage sensor (S4 region) inducing gating currents and pore opening.

33 G-proteins decreased the amplitude of gating currents and produced depolarizing shifts in the

34 es of channel gating such as the macroscopic gating current, and hence, it is presently unable to val

35 ode-shift was apparent by 24 ms at +60 mV in gating currents, and return of charge closely tracked po

36 Voltage-dependent binding and block of gating current are hallmarks of gating modifier toxins,

37 The properties of the gating currents are in excellent agreement with the prop

38 rack closely with each other, although ASAP1 gating currents are significantly faster than those of C

39 The defunct gating currents are similar in Shaker IR and these two m

40 kinetic steps affected by G-proteins because gating currents arise from the movement of voltage senso

41 ) many (but not all) details of the shape of gating current as a function of voltage.

42 This S4 translocation generates a gating current as the positively charged S4 basic residu

43 ion 666 (TM), affects both ionic current and gating current associated with channel activation, a pre

44 s fast component was found to be part of the gating current associated with the opening and closing o

45 oltage-dependent currents that represent the gating currents associated with CLC-5 activation and had

46 rent and also without blockers, by recording gating current at the reversal potential for ionic curre

47 stal N-terminus (Delta2-135) accelerated off-gating current, but did not influence the relative contr

48 did not shift the voltage-dependence of the gating currents, but reducing and increasing intracellul

49 Here, we show that Q1 gating current can be resolved in the absence of E1, but

50 mentary charge motions that give rise to the gating current cannot be observed directly, but appear a

51 Our results show that normal gating current cannot be stably recorded in the absence

52 and gating charge movement, slowing the OFF-gating current decay.

53 Saturating gating currents decreased by two-thirds (K(D) approximat

54 rlying channel gating behavior; by contrast, gating currents directly measure voltage-sensor displace

55 ting charge transfer nor the kinetics of the gating currents during activation.

56 Since the discovery of gating current, electrophysiologists have studied the mo

57 The resulting gating current exhibited a rising phase similar to that

58 The model computes gating current flowing in the baths produced by arginine

59 ave developed a method for rapidly computing gating currents from a multiparticle ion channel model.

60 uration mutagenesis and detailed analysis of gating currents from gating pore mutations in the Shaker

61 ited by site-3 toxins, we recorded ionic and gating currents from human heart Na channels with mutati

62 By measuring gating currents from the Shaker potassium channel, we de

63 ening transition was used to fit a series of gating currents from the Shaker potassium channel.

64 dent previously in the depolarization-evoked gating currents from voltage-gated Shaker K+ channels ha

65 ltaneously recorded fluorescence signals and gating currents from Xenopus laevis oocytes expressing A

66 The gating currents generated by the mutant channels were on

67 These so-called gating currents have been investigated extensively withi

68 Gating current (I(g)) recorded from a nonconducting muta

69 f thermal noise energy, kT, and to determine gating currents, I express the HH equations in the form

70 Sodium channel ionic current (INa) and gating current (Ig) were compared for rat skeletal (rSkM

71 e of STX, gating kinetics were measured from gating current (Ig).

72 ue to a dramatic slowing of the deactivation gating currents, Ig(D) (with tau up to 80 ms), which dev

73 An early component of the gating current in Shaker K+ channels with a time constan

74 es that allow us to simulate the fluctuating gating current in the activation processes.

75 ge stimulation of one cell in a group evokes gating currents in adjacent OHCs.

76 By measuring gating currents in BK channels coexpressed with chimeras

77 We examined changes in ionic and gating currents in Ca(V)1.2 channels when extracellular

78 is the first demonstration of sodium channel gating currents in single pituitary nerve terminals.

79 a functional permeation pathway by recording gating currents in the monomeric nonconducting D160N mut

80 and single-channel ionic currents as well as gating currents in tsA201 cells expressing Ca(V)2.2e[37a

81 To test this idea, we recorded NaV1.5 gating currents in Xenopus oocytes using a cut-open volt

82 Previous analysis of eag ionic and gating currents indicated that Mg2+ has a much larger ef

83 regulate the BK voltage sensor, we measured gating currents induced by the pore-forming BK alpha sub

84 o rethink voltage gating models in which the gating current is produced by physical motion of the S4

85 The time constant for the decay of off gating current is very similar to the time constant of d

86 Voltage-shift for gating currents is well documented for voltage-gated cat

87 S4 segment movement, as reflected in gating currents, is almost normal for a 4AP-occupied cha

88 Markov (DSM) model in describing ion channel gating current kinetics.

89 ects are slow compared with motility-related gating current kinetics.

90 Mg2+ has a much larger effect on ionic than gating current kinetics.

91 The gating currents lead to a voltage- and frequency-depende

92 mic-scale movements of charged groups to the gating current measured in an external circuit, however,

93 ue by directly detecting their movement with gating current measurements in 12 F(290) mutants.

94 Gating current measurements revealed that PIP(2) constra

95 teraction between Kv1.3 and NavBeta1 through gating current measurements using the Cut-open Oocyte Vo

96 In this work, we describe macroscopic and gating current measurements, together with molecular mod

97 Here, based on gating current measurements, we demonstrate that two arg

98 Here, using mutagenesis and gating current measurements, we show that a 3-fold accel

99 ation based on structural analysis and ionic/gating current measurements.

100 current pulses that generate fluctuations in gating current measurements.

101 Our gating currents measurements showed that NavBeta1 intera

102 The properties of the gating currents (nonlinear charge movements) of human ca

103 sodium conductance and 24.6 +/- 6.8% of the gating current of brain Na(v)1.2a channels, with a speci

104 The properties of ionic and gating currents of alpha 1E were investigated: ionic cur

105 sequence of potassium channels by analyzing gating currents of mutant Shaker IR channels and using t

106 CMMTSET to remove fast inactivation, and the gating currents of R1C-DIV-ICM(MTSET) were recorded befo

107 Gating currents of the voltage sensor are now known to d

108 ates accurately preserved the time course of gating currents on the slow timescale.

109 n the voltage dependence of motility-related gating current or, equivalently, on the voltage dependen

110 ained an alpha-periodicity index of 2.41 for gating current parameters, a new randomization test prod

111 observed in electrophysiology experiments as gating currents preceding ionic conduction.

112 t inactivation with potential alterations of gating current properties.

113 This behavior produces macroscopic gating currents quite similar to those experimentally fo

114 Gating currents recorded from Shaker and I470C were meas

115 By combining giant-patch ionic and gating current recordings in COS-7 cells, and voltage-cl

116 Gating current recordings reveal that mutations at R3 in

117 modulated by Mg2+, have not been detected in gating current recordings.

118 se of activation that is well represented in gating current recordings.

119 ional changes that are poorly represented in gating current recordings.

120 heir kinetics more closely resemble those of gating current records reported for ionic channels.

121 Gating current recovers from inactivation much faster th

122 There is a lag in the onset of gating current recovery at -80 mV, but no lag is discern

123 Our gating current results were reproduced with the addition

124 The model is closely based on the gating current studies of the preceding paper and has be

125 Gating current studies presented here indicate an abnorm

126 experimental data including ionic currents, gating currents, tail currents, steady-state inactivatio

127 ly, produce a distinct fast component in the gating current tails.

128 ctivation is much more voltage dependent for gating current than for ionic current.

129 two, and obtained the current-voltage curve, gating current, the response to a large sine wave (in th

130 HC mechanical activity, the motility-related gating current, to investigate mechano-electrical intera

131 egments-useful for assessing the macroscopic gating current-using the Fokker-Planck equation.

132 ttenuation in calcium current; the remaining gating current was no different in kinetics or voltage d

133 Interestingly, the "true" mode-shift of gating currents was approximately 40 mV, much greater th

134 By applying lidocaine and measuring the gating currents, we demonstrated that Asn residues in th

135 Gating currents were clearly resolved after ionic curren

136 The gating currents were integrated to measure the intramemb

137 WT and TM gating currents were isolated by replacing Ca2+ with the

138 rrents were measured in 10 mM external Ba2+; gating currents were isolated in 2 mM external Co2+.

139 ne the mechanism of this increase, ionic and gating currents were measured in transiently transfected

140 Gating currents were measured using a variety of inorgan

141 Calcium channel gating currents were recorded after the addition of 5 mM

142 Gating currents were recorded from a double mutant of Sh

143 S4 segment were replaced with histidine, and gating currents were recorded.

144 Gating currents were smaller in cells expressing only th

145 Gating currents were unaffected by verapamil.

146 cells to voltage change is accompanied by a 'gating current', which is manifested as nonlinear capaci

147 changes in the magnitude of motility-related gating currents, which are due predominantly to shifts i

148 rrents, which were modified strongly, and on gating currents, which were not detectably altered.

149 voltage sensor rearrangements with voltage (gating currents) whose movement and associated pore open

150 e was used, membrane depolarization elicited gating current with fast and slow components that differ

151 contains large barriers, which give rise to gating currents with two distinct time scales: the usual

152 n open duration, however, it accelerates off-gating currents without altering on-gating currents.