ライフサイエンスコーパス: gene of interest

1 y to explore the expression dynamics for any gene of interest.

2 e RNAs that target respectively HPRT and the gene of interest.

3 nitude of different noise sources in a given gene of interest.

4 ng the p63-dependent regulation of any human gene of interest.

5 r the design of highly active sgRNAs for any gene of interest.

6 plasmids, are extensively used to express a gene of interest.

7 specific cellular processes together with a gene of interest.

8 alian cells, to regulate the expression of a gene of interest.

9 used to examine a particular protein coding gene of interest.

10 sgRNAs for transcriptional repression of any gene of interest.

11 ng to assay for successful expression of the gene of interest.

12 cy and size of transcription bursts from the gene of interest.

13 serted into a gene-loading site along with a gene of interest.

14 ull-length 3'-UTR reporter construct for the gene of interest.

15 tation in the Crb1 gene rather than with the gene of interest.

16 to the cloning and transfer of just a single gene of interest.

17 The approach can be easily applied to any gene of interest.

18 laying the iron-mimic peptide and carrying a gene of interest.

19 tered to replace one of the viral genes by a gene of interest.

20 tion of mutations in almost any nonessential gene of interest.

21 ying potential zinc finger target sites in a gene of interest.

22 3 was used to extract optimal primers from a gene of interest.

23 e the resources available to investigate any gene of interest.

24 form the proper architecture to activate the gene of interest.

25 ion on shRNA constructs that can silence the gene of interest.

26 ose siRNA sequences that are specific to the gene of interest.

27 sing double-stranded RNA (dsRNA) targeting a gene of interest.

28 enhancers that regulate the expression of a gene of interest.

29 les engaged in the process of transcribing a gene of interest.

30 lly influence the expression of their target gene of interest.

31 tors which are likely to regulate a specific gene of interest.

32 to a relatively short expression time of the gene of interest.

33 limit the scope of orthology search for each gene of interest.

34 s) regulate the transcriptional process of a gene of interest.

35 ciated to their favorite cancer, drug and/or gene of interest.

36 difficult to assess regulation of any given gene of interest.

37 ntial expression and survival analysis for a gene of interest.

38 endogenous expression and regulation of the gene of interest.

39 targets thereby inactivating a co-expressed gene of interest.

40 approach to target and locally assemble the genes of interest.

41 ibility to study any custom miRNAs or target genes of interest.

42 of allowing researchers to focus on specific genes of interest.

43 morphisms in and around positional candidate genes of interest.

44 ic system to simultaneously express multiple genes of interest.

45 rogramming of the system to silence specific genes of interest.

46 n and validation, data triaging, and example genes of interest.

47 e and model organisms to selectively silence genes of interest.

48 dvantages that could be exploited to silence genes of interest.

49 on of fully virulent ECTV expressing foreign genes of interest.

50 miRNAs or tasiRNAs that potentially regulate genes of interest.

51 tion of target genes by suppressing specific genes of interest.

52 , and to contribute annotations for specific genes of interest.

53 s in the absence of genetic variation in the genes of interest.

54 disease phenotypes unrelated to the gene or genes of interest.

55 ion, users need to provide the GI IDs of the genes of interest.

56 ssary distance between promoter elements and genes of interest.

57 was performed, followed by validation of the genes of interest.

58 entifying plants carrying point mutations in genes of interest.

59 spatiotemporally control the perturbation of genes of interest.

60 of knocking down the expression of candidate genes of interest.

61 length polymorphism for polymorphisms in 10 genes of interest.

62 ter for imprecise excision screens to delete genes of interest.

63 ally change the search criteria to fit their genes of interest.

64 dation tags are fused onto the 3' end of the genes of interest.

65 drug-response and clinical implications for genes of interest.

66 this library, we determined expression of 27 genes of interest.

67 tissue material) sufficient to test > or =25 genes of interest.

68 ndings at regulatory regions associated with genes of interest.

69 sponsive elements) associated with potential genes of interest.

70 and analysis resulting in annotated lists of genes of interest.

71 ively fast technique to assess expression of genes of interest.

72 environmental bacteria carrying two or more genes of interest.

73 ify suitable TFO target sites within or near genes of interest.

74 ow possible to inactivate or delete selected genes of interest.

75 tified from transcription control regions of genes of interest.

76 lusters of BAC contigs in regions containing genes of interest.

77 m PR8-based viruses bearing the glycoprotein genes of interest.

78 gration of fluorescent markers to endogenous genes of interest.

79 ripts, including metabolic and adipocytokine genes of interest.

80 to submit FASTA files for their genomes and genes of interest.

81 the transposon insertion tolerance of normal genes of interest.

82 ifficulties in obtaining mutants in specific genes of interest.

83 the CAPS and dCAPS markers derived from the genes of interest.

84 ul for transient expression of various other genes-of-interest.

85 ws: (1) querying the regulatory network of a gene of interest, (2) prediction and interspecies transf

86 For any gene of interest, a probe that targets protein levels mu

87 that advancement starts in this paper with a gene of interest and a characteristic function of that g

88 of the need to re-calculate trees for every gene of interest and each time a new data set is analyze

89 generates a bicistronic message encoding the gene of interest and GFP, thus enabling identification o

90 tissue-related functions for any Arabidopsis gene of interest and predictions concerning the relatedn

91 b) clone of genomic material surrounding the gene of interest and use of this clone to establish a re

92 RNAi is used to systematically down-regulate genes of interest and analyze their roles in a biologica

93 Genes of interest and cell lineage markers were examined

94 ser-friendly interface for quickly searching genes of interest and downloading genome-wide results.

95 rget specific areas in the genome, including genes of interest and linkage regions, but this limits t

96 The user can input genes of interest and retrieve a list of piRNA targeting

97 By directing sequencing power to genes of interest and sensitively quantifying individual

98 This analysis confirmed translation of the genes of interest and showed that NKCC1, Na+,K+-ATPase a

99 ed on JavaScript, allowing the user to query genes of interest and visualize the cluster structure.

100 oolset allows for consistent transduction of genes of interest and will be a powerful molecular tool

101 le as 150 cells (as few as 2 transcripts per gene of interest) and perform mass-spectrometric analyse

102 rticular genomic region or associated with a gene of interest, and assign enhancers and their target

103 en type of epigenetic remodeling to a single gene of interest, and to probe the functional relevance

104 tiple genes, (ii) altering specific bases in genes of interest, and (iii) engineering genes to encode

105 , nine promoter:5'UTR fusions, 10 3'UTRs, 14 genes of interest, and 10 chloroplast-specific destinati

106 ned for a reference gene and one of a set of genes of interest, and builds a model VirtualEmbryo.

107 levels of expression similar to those of the genes of interest, and its expression was not modified b

108 s of potential primers that cover the entire gene of interest are then created and evaluated by Prime

109 io where the expression profiles of specific genes of interest are concentrated in a small cellular p

110 Second, because the genes of interest are not amplified by PCR, there are pr

111 s, (ii) genetically engineered mice in which genes of interest are overexpressed or deleted in a spec

112 cell signaling and the targeting of specific genes of interest are providing key insights into neurob

113 First, genes of interest are robustly identified in a pooled ge

114 al studies often rely on the expression of a gene of interest as transcriptional and translational fu

115 tists looking for functional clues for their genes of interest as well as for curators looking for in

116 ieved by repeating orthology searches on one gene of interest at a time, thereby generating a reduced

117 nthetic genes encode the same product as the gene of interest, but the synthetic nucleotide sequence

118 an epitope tag or a fluorescent protein to a gene of interest by CRISPR-Cas9-mediated homology-direct

119 ase is first targeted to the vicinity of the gene of interest by homologous recombination.

120 ene to be expressed in the same pattern as a gene of interest by placing the Gal4 transcription facto

121 ful, as they can be programmed to target any gene of interest by simply changing the sequence of the

122 sed to accommodate, at the given site, other genes of interest by "Recombinase-Mediated Twin Site Tar

123 ays, and results were confirmed for specific genes of interest by quantitative PCR.

124 ificantly over-represented in a given set of genes of interest, by comparison with a 'background' set

125 tens of thousands of base pairs flanking the gene of interest can be included in the construct.

126 minimally deleted regions in which relevant genes of interest can be found.

127 Choice of expression signals for the gene of interest, complications caused by the presence o

128 For the genes of interest, confirmation of gene expression was d

129 inexpensive small-scale arrays representing genes of interest could be used for many applications, i

130 is easily achieved at the genomic locus of a gene of interest due to the high efficiency of homologou

131 e who do - is how to inhibit the action of a gene of interest during development so as to learn about

132 etic fusion of the destabilizing domain to a gene of interest ensures specificity, and the attendant

133 f cell states, but the question "Where is my gene of interest expressed?" remains one of the most dif

134 However, intersection of extra-otic gene-of-interest expression with the CRE lineage can com

135 hiC31 and Bxb1 integrases was used to insert genes of interest flanked by phiC31 and Bxb1 attB sites

136 works well in creating mutations in specific genes of interest followed by complementation remains pr

137 ementary techniques identified C21orf91 as a gene of interest for susceptibility to HSL.

138 esses and allows for targeting of nearly any gene of interest for the creation of loss-of-function mu

139 he context of self-renewal and to prioritize genes of interest for experimental validation.

140 h the bacterial secretion system to activate genes of interest for functional analysis in plants.

141 e profiling can be translated to identifying genes of interest for functional tests in the future and

142 Genes of interest for further functional analysis were s

143 sis, and Gene Carts that allow users to save genes of interest for further study while they browse.

144 Recombinant rabies viruses can encode genes of interest for labeling cells, controlling gene e

145 ector that allows a selective isolation of a gene of interest from genomic DNA in yeast followed by i

146 trix which organizes a dataset of over 2,000 genes of interest from a genome wide scan of transcripti

147 arison of protein domain organization within genes of interest from metagenomes and complete microbia

148 approaches including specific knockdowns of genes of interest from primary CD34(+) hematopoietic ste

149 the challenge for biologists is to identify genes of interest from the thousands of genetic expressi

150 ng ES cell clones with mutations in specific genes of interest from this library.

151 The human gene of interest, GJB1, which is mutated in X-linked Cha

152 sion in 3D by inducing expression of a novel gene of interest, glycoprotein nonmetastatic B (GPNMB),

153 emid (PM) library expressing variants of the gene of interest (GOI) and preparation of the Escherichi

154 ndent on the formulation, translation of the gene of interest (GOI) inserted into the RepRNA (lucifer

155 Retroviral (RV) expression of genes of interest (GOIs) is an invaluable tool and has f

156 ing) to insert fluorescent protein tags into genes of interest harbored by transformation-competent b

157 archers use the PSAT web interface to find a gene of interest in a reference genome and efficiently r

158 hnique that can induce mutations in a target gene of interest in almost any mammalian cell line.

159 fers the possibility to knock out almost any gene of interest in an affordable and simple manner.

160 Delivering and expressing a gene of interest in cells or living animals has become a

161 wser', that shows the read map coverage of a gene of interest in each of the samples along with 'elec

162 the absolute number of mRNA molecules from a gene of interest in individual, chemically fixed Escheri

163 PPP4R3A should be further investigated as a gene of interest in neurodegenerative diseases and as a

164 lly PCR-amplifying 150-350 bp fragments of a gene of interest in situ) and specificity (derived from

165 to study the dynamic molecular effects of a gene of interest in the pluripotency network.

166 getable, allows high-level expression of any gene of interest in the synaptically coupled neurons, an

167 usly identified IFI44 and IFI44L as possible genes of interest in a bioinformatic screen, we dissecte

168 idual cells, expressing or carrying specific genes of interest in a latent form in a tissue section u

169 as for the selective knock-down of specific genes of interest in a tissue-selective manner.

170 within months) addressing the function(s) of genes of interest in a wide variety of specific cell typ

171 We interrogated genes of interest in an interval on mouse chromosome 10

172 re can be used to evaluate other therapeutic genes of interest in animal models before future clinica

173 accurate identification of organisms or even genes of interest in complex environmental samples (air,

174 c biochip capable of accurately quantitating genes of interest in DNA samples.

175 Two genes of interest in H. akashiwo, not previously reporte

176 This approach enables efficient deletion of genes of interest in major immune lineages without alter

177 to determine the expression profile of their genes of interest in multiple clinical data sets and sev

178 to specifically repress the transcription of genes of interest in plants.

179 Genes of interest in psoriasis tissue that did not retur

180 xplore the functional relationships of their genes of interest in specific mouse tissue contexts.

181 uccessfully used AutoCloner to clone various genes of interest in the Apogee wheat variety, which has

182 ling and provides a means of rapidly editing genes of interest in the heart.

183 s available for conditionally overexpressing genes of interest in the MK/platelet lineage in vivo.

184 tworks, eQTL mappings and expressions of key genes of interest in VC and VD functions.

185 hat our new visualization tools can identify genes of interest in ways undetectable with models.

186 tosidase gene, as a cellular reporter, and a gene of interest (in this case, the mitotic regulator Au

187 The genes of interest include known and novel genes encoding

188 Genes of interest include those involved in the pharmaco

189 Genes of interest included a 16S rRNA gene selective for

190 Single genes of interest included I-kappa-B kinase-alpha (CHUK)

191 tifying expression-independent properties of genes of interest including upstream transcriptional reg

192 h the BDP, the evolutionary half-time of the gene of interest increases 4-10 times, depending on the

193 ogical samples increases or as the number of genes of interest increases, respectively, and these fac

194 A method is presented to assemble a gene of interest into a linear DNA template with all the

195 utput promoter to control transcription of a gene of interest into B. subtilis.

196 he procedure involves, first, cloning of the genes of interest into an appropriate mini-Tn7 vector; s

197 he procedure involves, first, cloning of the genes of interest into an appropriate mini-Tn7 vector; s

198 he procedure involves, first, cloning of the genes of interest into an appropriate mini-Tn7 vector; s

199 viruses have been widely employed to deliver genes of interest into pancreatic islets.

200 ration of VIGS vectors carrying fragments of genes of interest into seedlings at the two- to three-le

201 Particular genes of interest involved in embryogenesis (abscisic ac

202 iable cost-effective approach, providing the gene of interest is adequately captured.

203 A gene of interest is disrupted in adult floxed mice by a

204 s particularly useful in instances where the gene of interest is essential and a knockout mouse is no

205 e applied effectively to determine whether a gene of interest is essential, perform functional analys

206 ry because loss (or gain) of one copy of the gene of interest is insufficient to recapitulate the dis

207 cassette is uniquely arranged such that the gene of interest is split and only reconstituted in the

208 In our method, PCR-amplified sequence of a gene of interest is used as a template for the synthesis

209 We demonstrate that when the number of genes of interest is inconveniently large, identifying a

210 approach employs deletion of a nonessential gene of interest (knockout), or multi-step recombineerin

211 A sequences from both sides of a transgenic "gene of interest," leaving only the desired gene and sho

212 the study of the inner ear is that loss of a gene of interest may cause embryonic lethality before th

213 However, it is possible that a gene of interest may have expression that is neither cyc

214 quorum sensing systems which, coupled to any gene of interest, may facilitate the translation of circ

215 For genes of interest, messenger RNA (mRNA) induction in car

216 a protocol in which the in vivo effects of a gene of interest on both B and T lymphocyte development

217 In order to generate HACs carrying a gene of interest, one can either co-transfect the alphoi

218 to prepare a mouse model that expresses the gene of interest only in the liver, but not in other tis

219 Users can find a particular gene of interest or a cluster of genes that behave simil

220 e through which researchers can search for a gene of interest or groups of genes by Gene Ontology cat

221 umerous functional siRNA constructs from any gene of interest or pool of genes.

222 nctional relationships for their tissues and genes of interest or examine gene function and interacti

223 cloning sites, and inability to express the genes of interest or purify the protein products.

224 ataset can be used to explore DTU events for genes of interest or to find genes with specific DTU in

225 either co-transfect the alphoid DNA and the gene of interest, or one can clone both into a single ve

226 that directs the inhibition of a particular gene of interest, potentially producing a specific pheno

227 ive gene expression presents the data of the gene of interest relative to some calibrator or internal

228 To infer transcription kinetics for a gene-of-interest, researchers commonly compare the distr

229 Embedding a toxin gene in a gene of interest restricted its horizontal propagation.

230 SpCas9 (dSpCas9) to the region upstream of a gene of interest, resulting in a position-dependent, but

231 For any input gene of interest, SAPTA gives a ranked list of potential

232 Users can retrieve elements near single genes of interest, search for enhancers that target repo

233 Investigators can interrogate genes of interest, search for the genes that show the st

234 ogists to assess connectivity among a set of genes of interest ('seed-genes') within a biological net

235 ency for clustering was found common for the genes of interest, significant variations in gene order

236 Other genes of interest significantly altered in the presence

237 Current strategies involve modifying a gene of interest such that a degradation peptide is adde

238 e features of the pan-genome, detect sets of genes of interest such as antimicrobial resistance (AMR)

239 physically or functionally with the initial gene of interest than have SSNC-based screens.

240 Second, genes of interest that capture significant responses to

241 ge and data with a global F-test for finding genes of interest that minimizes the need for replicates

242 alter the level of mRNA transcribed from the gene of interest, the assumption is and always has been

243 Mice lacking one of the genes of interest, the apoptosis/necroptosis gene RIPK3,

244 For the 170 genes of interest, the DNA coverage was greater than 20x

245 For genes of interest, the user can view baseline expression

246 ntify multiple gene effects in two candidate genes of interest--the serotonin transporter gene (SLC6A

247 Of these, half do not impact the candidate gene of interest; the other half correlate with expressi

248 hod focusing single-cell RNA-seq coverage on genes of interest, thereby increasing the sensitivity an

249 uires just one variable sgRNA to target each gene of interest, this procedure can be implemented with

250 medical researchers with new tools to filter genes of interest, thus, reducing costly lab studies.

251 ated a modular platform (pMVP) that allows a gene of interest to be studied in the context of an arra

252 y 70 kb of alphoid DNA to any BAC carrying a gene of interest to generate a HAC vector.

253 uses viral vectors carrying a fragment of a gene of interest to generate double-stranded RNA, which

254 In order to precisely target expression of a gene of interest to particular cell types, intersectiona

255 ler that can adapt the expression level of a gene of interest to significant resource loading in mamm

256 d were shown to be capable of delivering the gene of interest to target cells where it was translated

257 three case studies, ranging from querying a gene of interest to the identification of gene networks

258 f the parasite genome to disrupt or modify a gene of interest to understand its importance and functi

259 idely used to 'knock-down' the expression of genes of interest to explore phenotypes.

260 community to identify other cell states and genes of interest to expose the underpinning of developm

261 particular gene and to link any two or more genes of interest to single species residing in complex

262 thod to easily make translational fusions of genes of interest to the lacZ reporter gene, under the c

263 c guide (g)RNAs to regulatory regions of any gene of interest, to enhance transcription.

264 he isolation of cDNA clones for a particular gene of interest, to the improvement of large gene colle

265 out), or multi-step recombineering to move a gene of interest under a repressible promoter (knockdown

266 results in near-immediate transcription of a gene of interest under physiological conditions.

267 ally selected and result in placement of the gene of interest under the control of new regulatory ele

268 lective isolation of any genomic fragment or gene of interest up to 250 kb in size from complex genom

269 and manipulation of viral genomes to express genes of interest (vectors 1.0).

270 b server through which users can query their genes of interest, visualize the network, gain functiona

271 In most of these studies inactivation of the gene of interest was associated with either glucose into

272 g version of the repressor plasmid while the gene of interest was delivered in an integrating piggyBa

273 choriomeningitis virus (LCMV) to express two genes of interest was recently reported.

274 with targeted selection of mutations in the genes of interest, was successfully applied to the gene

275 f a series of mutations on the function of a gene of interest we have generated three vector series t

276 carrying a different gene drive disabling a gene of interest, we are able to create diploid strains

277 t DNase I hypersensitive sites surrounding a gene of interest, we carried out both loss- and gain-of-

278 ows ligand-dependent, efficient removal of a gene of interest, we generated three knockouts of apicom

279 h, DNA was extracted from dried blood spots, genes of interest were amplified, amplicons were subject

280 Genes of interest were disrupted in 5 to 15% of preselec

281 Over 90 genes of interest were identified, and 35 of 44 tested w

282 Many genes of interest were involved in biological functions

283 h Ct serovar E, and changes in expression of genes of interest were measured using RT-PCR, proteomic

284 evated hepatic transcriptional levels of the genes of interest were noted in congenic C57BL/6J-Ah(d)

285 The genes of interest were potently and readily silenced wit

286 Two of the most highly dysregulated genes of interest were stresscopin, a neuropeptide invol

287 Additional differentially expressed genes of interest were those involved in the cytolytic p

288 Within each patient, the 18 genes of interest were used to calculate scores for dist

289 transgenic mice that overexpress one of the genes of interest were used to reveal genes involved in

290 rphisms in the 5' untranslated region of the gene of interest when carrying out antisense morpholino

291 nditional knockout model for each individual gene of interest where two loxP sites must be simultaneo

292 nked with directly oriented loxP sites and a gene of interest, which are introduced into plastids by

293 re initiated with the sequence of the user's gene of interest, which is searched against a database o

294 of embryos carrying mutations in a targeted gene of interest, which should allow for rapid screening

295 or allele-selective phenotypic assays of the gene of interest while it remains expressed and regulate

296 rategy allows easy and efficient delivery of genes of interest whose expression levels require regula

297 oaches have shown promise in contextualizing genes of interest with known molecular interactions.

298 om the same promoter, DD-Cas9 with any other gene of interest without co-modulation of the latter.

299 igh, allowing for a complete deletion of the gene of interest, without undesirable illegitimate integ

300 t LCMV (rLCMV) expressing additional foreign genes of interest would open novel avenues for the study