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1 sitive samples (i.e. experimentally verified glycosylation sites).
2 five silent sites in addition to the normal glycosylation site.
3 extensive heterogeneity associated with each glycosylation site.
4 of this viral strain lacks the new putative glycosylation site.
5 en to modify the mouse genome to remove this glycosylation site.
6 rands, with the former burying one prominent glycosylation site.
7 tides with glycans attached to each specific glycosylation site.
8 subclasses and heterogeneities at each head glycosylation site.
9 he N38S amino acid change and a loss of an N-glycosylation site.
10 main, was not affected by mutation of this N-glycosylation site.
11 envelope proteins or introduction of a novel glycosylation site.
12 cale mapping of Tn-glycosylated proteins and glycosylation sites.
13 SIGN is better correlated with the number of glycosylation sites.
14 he constant domains and disrupting predicted glycosylation sites.
15 acid substitutions and continual addition of glycosylation sites.
16 rved N-glycan or the deletion of predicted O-glycosylation sites.
17 e high evolutionary conservation of all four glycosylation sites.
18 but failed to detect those further from the glycosylation sites.
19 98 degrees C), and absence of cysteines and glycosylation sites.
20 se is glycosylated at all twelve potential N-glycosylation sites.
21 Exon 16 also includes two O-glycosylation sites.
22 h of gp120 at the Asn332, Asn392, and Asn386 glycosylation sites.
23 roxylases (P4Hs), defines their subsequent O-glycosylation sites.
24 sion protein that contains multiple N- and O-glycosylation sites.
25 beta-hCG bearing model glycans at all native glycosylation sites.
26 g glycan structures with their corresponding glycosylation sites.
27 was then used to pinpoint the 12 occupied O-glycosylation sites.
28 O-linked glycans and located the occupied O-glycosylation sites.
29 a reesei Family 7 cellobiohydrolase at three glycosylation sites.
30 hat of JR-FL or with mutations in putative N-glycosylation sites.
31 enaviruses is glycosylated at 11 conserved N-glycosylation sites.
32 n sites, with acquisition of around 10 new O-glycosylation sites.
33 within MIC2, with >95% occupancy at these O-glycosylation sites.
34 ave shorter variable loops or fewer N-linked glycosylation sites.
35 glycosylated and contains numerous O- and N-glycosylation sites.
36 ependent test for predicting human and mouse glycosylation sites.
37 s were found to have a dramatic influence on glycosylation sites.
38 hages transfected with FcgammaRIa in which N-glycosylation sites 1, 4, and 5 are mutated to alanines
43 oward a differential selection pressure of N-glycosylation site acquisition during affinity maturatio
45 mapping and comparing variable loop N-linked glycosylation sites across populations of HIV-1 sequence
46 Moreover, classical cadherins have multiple glycosylation sites along their extracellular regions.
47 antibody sensitivity, and putative N-linked glycosylation sites along with a comprehensive sequence
48 f gp120, here we report the first systematic glycosylation site analysis of gp120 derived from virion
49 tionic 6-deoxymannose sugar at the canonical glycosylation site and a second sugar, an unusual 4-O-me
50 igns of an immunogenic region to add a new N-glycosylation site and mask it from antibody binding.
51 ed for extracting DIA data is common to that glycosylation site and not dictated by a specific MS1 va
52 able the identification of peptide sequence, glycosylation site and the structure of intact glycopept
54 Furthermore, it included two additional N-glycosylation sites and a pair of cysteines suggestive o
55 esults demonstrate a critical role for the N glycosylation sites and cysteines for the structure and
56 -III, di-sialylated glycans at multiple A1AT glycosylation sites and desialylated A2HSG, and depleted
57 have enabled the identification of specific glycosylation sites and glycan structures that modulate
58 correlates inversely with putative N-linked glycosylation sites and positively with virion infectivi
59 almost no correlation between the number of glycosylation sites and protein length, but the number o
63 shown to be heavily N-glycosylated, but the glycosylation sites and the biological role of N-linked
65 lating H3N2 viral strain (that possesses the glycosylation site) and humans vaccinated with baculovir
66 eneity (different glycoforms attached to one glycosylation site) and macroheterogeneity (site occupan
67 fied as a 73 amino acid protein having one N-glycosylation site, and a variant 74 residue non-glycosy
69 edicted secretion leader sequences, N-linked glycosylation sites, and a putative site of tyrosine sul
70 uence coverage, including seven of the eight glycosylation sites, and has been used to study the impa
71 he K(2P) channels possess consensus N-linked glycosylation sites, and here we demonstrate that the co
72 in the stalk region abolishing the N-linked glycosylation site; and 2 variants represented the head
73 elucidation of the glycan structure and the glycosylation site are needed to reveal the biological f
74 ested that mutations and changes in N-linked glycosylation sites are associated with the rise of anti
76 NA head domain of H1N1 IAVs, three N-linked glycosylation sites are conserved and that a fourth site
77 of 25, 22, and 34 glycopeptides covering all glycosylation sites are enriched by the modified tips fr
78 Knowing the Tn-glycosylated proteins and glycosylation sites are essential to the prevention, dia
79 ons in both the number and distribution of N-glycosylation sites are found in the 18 alpha and 8 beta
81 lastidal reporter proteins with artificial N-glycosylation sites are indeed glycosylated during trans
82 t form, three of the four potential N-linked glycosylation sites are modified by carbohydrate attachm
83 large portion of negative samples (i.e. non-glycosylation sites) are mislabelled due to the limitati
84 lidated the congruence of the potential HA N-glycosylation sites as well as the presence of the HA pe
85 y of previously unobserved putative N-linked glycosylation sites, as well as codons that evolve under
89 1)NDS N-glycosylation motif, but not other N-glycosylation sites (Asn(260), Asn(371), and Asn(394)),
93 pies Cys200/Cys209 residues, one of the SERT glycosylation sites, Asp208 located between the two Cys
95 d an incentive to modify the unique N-linked glycosylation site at Asn(297), either through chemical
96 nsitive to the presence of a single N-linked glycosylation site at asparagine 297 of the Fc, with deg
97 epeats contain an evolutionarily conserved O-glycosylation site at position -1 of the first cysteine
98 th VLPs expressing HA antigens that lacked a glycosylation site at residue 144 and a deleted lysine a
101 ion of an additional fully occupied N-linked glycosylation site at the N terminus at position 1 (equi
102 milarity with beta-hLH, including a common N-glycosylation site at the N-terminus but differs mainly
103 sylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289.
105 dentification and orthogonal validation of N-glycosylation sites based on alternating sequential samp
107 quantification of glycosylated peptides and glycosylation sites but not glycosylation occupancy or g
108 eted protein was due not to the loss of an N-glycosylation site, but rather an SNP-specific targeting
109 red for histamine catabolism, has multiple N-glycosylation sites, but their roles, for example in DAO
110 ein abundance but also the occupancy of each glycosylation site by different glycoforms during biolog
111 ssess the relative occupancies of numerous N-glycosylation sites by endoglycosidase H-resistant N-gly
112 enetically introducing aspartates at these N-glycosylation sites bypasses the requirement for PNG-1/N
113 an side chain remains intact in ETD, and the glycosylation site can be localized on the basis of the
114 e that in addition to antigenicity, N-linked glycosylation sites can alter NA enzymatic stability and
115 ddition to antigenicity, changes in N-linked glycosylation sites can alter other properties of viral
116 location and heterogeneity of surrounding N-glycosylation sites can be altered, resulting in exposur
117 carrying key hemagglutinin (HA) head region glycosylation sites can be removed from the lung by pulm
118 in PNS myelin and mutations in which at the glycosylation site cause Charcot-Marie-Tooth neuropathy,
122 oplasmic reticulum or mutation of the Asn-24 glycosylation site decreased GC activity, but neither in
123 uences into NCAM Ig5, including an "extra" N-glycosylation site, decreases or completely blocks NCAM
124 ng approaches have been proposed to identify glycosylation sites, demonstrating a promising predictiv
125 al elimination of evolutionarily conserved N-glycosylation sites did not abolish proper KOR1 folding,
127 We suggest that the occasional absence of glycosylation sites encoded in the conserved regions of
128 verall selection bias against acquiring an N-glycosylation site, except for the CDR3 of the H chain.
129 t at least seven C-linked and three O-linked glycosylation sites exist within MIC2, with >95% occupan
130 IgM exhibits different accessibility of the glycosylation sites, explaining site-specific glycosylat
131 Vs of human origin, where the number of head glycosylation sites first increased over time and then d
133 expose the CD4bs by selective elimination of glycosylation sites flanking the CD4bs, and 2) minimize
134 rked by analyzing Jurkat cells, where 947 Tn-glycosylation sites from 480 glycoproteins were mapped.
135 s focus mainly on either the de-glycosylated glycosylation site (glycosite)-containing peptides or th
137 we confirmed that such mutations at multiple glycosylation sites greatly diminish viral infectivity a
139 n and swine origin, where the number of head glycosylation sites has mainly increased over time.
140 LC/MS(E) glycomics analysis revealed that glycosylation sites have specific proportions of N-glyca
143 C.2a H3N2 viruses possessing a new predicted glycosylation site in antigenic site B of HA emerged, an
144 e ~10 amino acids that surround the Asn(154) glycosylation site in each of the 180 envelope glycoprot
145 ession and that a mutation that eliminates a glycosylation site in HA(1) allows the Israel810 HA to g
146 ion CLRN1(N48K), which affects a conserved N-glycosylation site in hCLRN1, is a common causative USH3
147 we demonstrate that the conserved putative N-glycosylation site in K(2P)3.1 and K(2P)9.1 is a glycan
149 ontemporary ZIKV isolates encode an N-linked glycosylation site in the envelope (E) protein (N154), b
150 sequence motif, VNDT, containing an N-linked glycosylation site in the envelope (E) protein, is polym
151 um channels have a highly conserved N-linked glycosylation site in the first extracellular loop, with
152 of glycosylation at both or the first PrP(C) glycosylation site in the host results in almost complet
154 t and N-glycosylation analysis identified 73 glycosylation sites in 65 aleurone layer proteins, with
155 l-amino acid cassettes into closely spaced O-glycosylation sites in a single, high-yielding solid-sup
156 current study is to establish patterns of N-glycosylation sites in Ab V regions of naive and memory
157 in the greatest number of glycoproteins and glycosylation sites in biological duplicate experiments.
158 are associated with the presence of multiple glycosylation sites in close proximity and large structu
160 The determination of N- and O-glycans and O-glycosylation sites in etanercept provides a basis for f
163 ortance of the conserved cysteines and the N-glycosylation sites in NBCe1-A EL-3, we analyzed the pot
165 y machinery, including the identification of glycosylation sites in secreted proteins, an important g
166 upon complex oligosaccharide processing of N-glycosylation sites in the amino-terminal domain and dow
167 e investigate the contribution of individual glycosylation sites in the formation of this so-called i
169 homa (FL), but not normal B cells, acquire N-glycosylation sites in the immunoglobulin variable regio
174 ly shown that ablation of the three N-linked glycosylation sites in the West Nile virus NS1 protein c
175 f HIV-1 sequence alignment by using N-linked glycosylation sites in variable loops as alignment ancho
178 s suggest that the loss of the N173 N-linked glycosylation site increases SIVmac239 replication in ma
181 merization could be abolished by engineering glycosylation sites into the dimer interface; other inte
182 in the envelope (E) protein (N154), but this glycosylation site is absent in many historical ZIKV iso
183 DT motif is deleted or in which the N-linked glycosylation site is mutated by single-amino-acid subst
184 roheterogeneity of glycopeptides identifying glycosylation sites is a challenging task, and there is
185 oteotype and identification of extracellular glycosylation sites is challenging when samples are limi
186 an species from both the Fab and Fc N-Linked glycosylation sites is consistent with quantification us
187 e-rich protein that contains STT3A-dependent glycosylation sites, is poorly glycosylated in yeast cel
188 the IgG sequence, in addition to unambiguous glycosylation site localization and extensive coverage o
190 to routinely assess the glycan type at each glycosylation site may facilitate design of improved vac
191 nity for EphA2, indicating that the EBV gL N-glycosylation site might be responsible for inhibiting t
194 single N-acetylglucosamine at each of the N-glycosylation sites [monoglycosylated HA (HA(mg))] can e
195 irus-expressed H3 antigens (that possess the glycosylation site motif) were able to efficiently recog
196 he cellular and supernatant fractions, while glycosylation site mutants did not adversely affect viri
198 atory sequences established that all eight N-glycosylation sites (N1 to N8) are used in the wild type
200 xtracellular domain of KOR1 contains eight N-glycosylation sites, N1 to N8, of which only N3 to N7 ar
201 229) is needed for TC-Cbl binding, but the N-glycosylation sites (N126, N195, and N213) are of no imp
202 A2m(1), the engineered molecule lacked two N-glycosylation sites (N166 and N337), two free cysteines
203 ere, we identify a highly conserved N-linked glycosylation site (N173 in SIV, corresponding to N160 i
205 68T), with P68T generating a second N-linked glycosylation site (N66) in addition to an invariant N32
208 ses contains 2 completely conserved N-linked glycosylation sites (NGSs) at N563 and N618, suggesting
213 of-function mutation (S562L) in a putative S-glycosylation site of GmCLV1A causes stem nodal identity
214 e how hemophilia mutations near the unused N-glycosylation site of the A2 domain (N582) of FVIII affe
216 r indicated that, among the four potential N-glycosylation sites of E-cadherin, Asn-554 is the key si
217 vation of any of the four predicted N-linked glycosylation sites of Ly49B, nor was it affected by rem
220 Asn-to-Ala substitutions at the predicted N-glycosylation sites of the M41-RBD were evaluated along
221 is heavily glycosylated at approximately 25 glycosylation sites, of which approximately 7-8 are loca
222 sites, a third novel, albeit low abundant, N-glycosylation site on C9 is identified, which surprising
225 n chimpanzee CD4 (68T) that creates a second glycosylation site on the same virus-binding interface.
226 e-specific substitution (34T) that creates a glycosylation site on the virus binding surface of the C
228 lly triplicate experiments, on average 953 N-glycosylation sites on 393 surface glycoproteins per exp
229 s spectrometry-based technique for mapping O-glycosylation sites on herpes simplex virus type 1.
230 O cells, the number of N-linked and O-GalNAc glycosylation sites on individual host cell proteins (HC
231 rein, we have systematically characterized O-glycosylation sites on recombinant LDLR shed from HEK293
232 dentification, delineating multiple N- and O-glycosylation sites on single glycopeptides, and derivin
233 uantification results demonstrated that many glycosylation sites on surface proteins were down-regula
235 d of heavily glycosylated polymers with five glycosylation sites on the mu (heavy) chain and one glyc
236 N) showed that the conservation of N-linked glycosylation sites on the NA enzymatic head domain diff
237 nd N2 mutants demonstrated that the N-linked glycosylation sites on the NA head domain are required f
238 rimental analyses verified that the N-linked glycosylation sites on the NA head domain contribute to
241 Ab molecule containing two distinct N-linked glycosylation sites: one present on the heavy chain (HC)
243 are distinguished by compact Envs with fewer glycosylation sites, our study points to fusion and poss
251 sylation, and particularly the enrichment in glycosylation sites proximal to the disulfide linkages a
254 re were no differences in protease cleavage, glycosylation sites, signal peptides or trans-membrane d
255 ce of protease cleavage sites, Pfam domains, glycosylation sites, signal peptides, trans-membrane pro
256 rds understanding the role of these putative glycosylation sites, site-directed mutagenesis and lecti
257 intact glycopeptides corresponding to eight glycosylation sites (six N-linked and two O-linked sites
258 s at sites 34 and 68, destroying both of the glycosylation sites, suggesting that the effects of the
259 ionally, we report a newly observed O-linked glycosylation site, T606, and we show that the full O-li
261 ence of multiple phosphorylation sites and a glycosylation site that we find to be occupied by at lea
262 riable regions (V1-V5), and also at N-linked glycosylation sites that contribute roughly half the mas
264 ligand receptor site that is ringed by four glycosylation sites that dramatically impact ligand bind
265 mplex-type biantennary N-glycans linked to N-glycosylation sites that emerge during somatic hypermuta
266 Therefore, for proteins containing multiple glycosylation sites, the individual glycan species prese
267 f cysteines in Env, and 4 potential N-linked glycosylation sites; the top features will be advanced t
269 ucted the largest dataset of human and mouse glycosylation sites to train deep learning neural networ
270 this study, we tried to identify atypical N-glycosylation sites using our recently developed solid-p
271 Interestingly, beta2 with a single intact glycosylation site was as effective as the WT in promoti
273 harides and the type of substitution at each glycosylation site was determined by chemical, spectrosc
275 contrast, a protein with extreme C-terminal glycosylation sites was efficiently glycosylated in yeas
276 otein-derived epitope to contain an N-linked glycosylation site, we determined that optimal CD8(+) T
277 to decipher the function of its potential N-glycosylation site, we produced pro-KLK2 in Leishmania t
278 AXL) as a model glycoprotein with multiple N-glycosylation sites, we show that those LCMS features th
284 )17.1 channels and channels lacking specific glycosylation sites were expressed in Xenopus laevis ooc
285 ides derived from 56 glycoproteins with 83 N-glycosylation sites were identified from human serum and
286 es derived from 129 glycoproteins with 157 N-glycosylation sites were identified from mouse liver tis
287 dic N-linked oligosaccharides, the six other glycosylation sites were modified with complex N-glycans
288 series of ORF2 mutants in which the three N-glycosylation sites were mutated individually or in comb
290 rometry demonstrated that all 11 predicted N-glycosylation sites were utilized in both HEK293- and Sf
291 spectrometry demonstrated that the predicted glycosylation sites were utilized in both mammalian and
292 L is at least in part proximal to the EBV gL glycosylation site, which in part accounts for differenc
293 in the native hosts, but contains potential glycosylation sites, which are aberrantly glycosylated d
294 HDX changes focused around the N221 and N255 glycosylation sites, which contain mannose-6-phosphate m
296 Several amino acid changes altered predicted glycosylation sites, with acquisition of around 10 new O
297 re was considerable variation in potential N-glycosylation sites, with GA2 and ON1 viruses showing up
298 rom the recent outbreaks contain an N-linked glycosylation site within the viral envelope (E) protein
299 alyzed the distribution and acquisition of N-glycosylation sites within Ab V regions of peripheral bl
300 ous system function - contain two putative N-glycosylation sites within the large N-terminal domain a