ライフサイエンスコーパス: guard cell

1 transport across the membrane system of the guard cell.

2 lies on quantitative systems analysis of the guard cell.

3 ced by abscisic acid and highly expressed in guard cells.

4 (C3 plants), bundle-sheath (C4 plants), and guard cells.

5 hich is one of the phototropin substrates in guard cells.

6 y a reduced accumulation of K(+) ions in the guard cells.

7 n of signal amplification and specificity in guard cells.

8 ith its putative paralog SINE2, expressed in guard cells.

9 rimary and specialized metabolic pathways in guard cells.

10 regulation of salicylic acid (SA) pathway in guard cells.

11 dynamics, gas exchange, and ion transport of guard cells.

12 efense and is linked to hormone signaling in guard cells.

13 g autonomous red light signaling pathways in guard cells.

14 xpression from bundle sheath to mesophyll to guard cells.

15 idermal cells facilitating ion supply to the guard cells.

16 en by solute accumulation in the surrounding guard cells.

17 nflux and K(+) efflux across the PM of Col-0 guard cells.

18 he understanding of CO2 signaling pathway in guard cells.

19 g several pavement cells adjacent to the two guard cells.

20 a core pathway for CO(2) signalling in plant guard cells.

21 ith its function as an ABA efflux carrier in guard cells.

22 he activity of AtCLCa in vivo in Arabidopsis guard cells.

23 he control of nuclear sizes in trichomes and guard cells.

24 orters results in solute accumulation in the guard cells.

25 anism in ABA and MeJA signal transduction in guard cells.

26 ristics in tobacco epidermis and Arabidopsis guard cells.

27 es in the nuclear size in both trichomes and guard cells.

28 rectifying Kv-like K(+) channel expressed in guard cells.

29 idence that basal SnRK2 activity prevails in guard cells.

30 in N. benthamiana leaf cells and Arabidopsis guard cells.

31 tion induces LCBK1 expression, especially in guard cells.

32 ll pores on plant leaves and stems formed by guard cells.

33 on of NCED3, a key step of ABA synthesis, in guard cells.

34 which is expressed in expanding tissues and guard cells.

35 While DELLA had no effect on ABA levels, guard cell ABA responsiveness was increased in S-della a

36 matal aperture, this occurs by accessing the guard cell ABA signaling pathway.

37 2.6 (SnRK2.6), a protein kinase involved in guard cell ABA signaling, was able to phosphorylate a cy

38 ces stomatal closure via QUAC1/ALMT12 and/or guard cell ABA synthesis.

39 o red light is correlated with a decrease in guard cell abscisic acid content and an increase in jasm

40 In intact guard cells, abscisic acid (ABA) enhances (primes) the C

41 We measured guard cells across the genera with stomata to assess dev

42 to darkness is mediated by reorganisation of guard cell actin filaments, a process that is finely tun

43 A new study makes it clear that guard cells also metabolise starch to accelerate opening

44 Stomatal pores are formed between a pair of guard cells and allow plant uptake of CO2 and water evap

45 ced phospholipid uptake at the root tips and guard cells and are affected in growth and transpiration

46 maximal in the mesophyll compared with both guard cells and bundle sheath.

47 localized synthesis of stilbenes in stomata guard cells and cell walls is induced by P. viticola inf

48 Guard cells and epidermal cells of hornworts show striki

49 coronatine, which blocks the functioning of guard cells and forces stomata to reopen.

50 (2)-induced activation of Ca(2+) channels in guard cells and is required for stomatal closure.

51 receptor JAZ2 is constitutively expressed in guard cells and modulates stomatal dynamics during bacte

52 he increased number of small cells below the guard cells and of fully developed stomata indicated tha

53 ne localization patterns when imaged in both guard cells and pollen.

54 ase gene, PME6, which is highly expressed in guard cells and required for stomatal function.

55 K(+) channels of tobacco (Nicotiana tabacum) guard cells and show its close parallel with stomatal cl

56 n involves limited separation between sister guard cells and stomatal responses require reversible gu

57 was observed in the whole stomatal complex (guard cells and subsidiary cells), root vasculature, and

58 establish a link between gene expression in guard cells and their cell wall properties, with a corre

59 ases in response to low humidity and NaCl in guard cells and to NaCl and osmotic stress in roots and

60 l closing and whether starch biosynthesis in guard cells and/or mesophyll cells is rate limiting for

61 rane H(+)-ATPase AHA1 is highly expressed in guard cells, and its activation can induce stomatal open

62 pecies Kalanchoe fedtschenkoi, we found that guard cell anion channel activity, recorded under voltag

63 the hormone that leads to the activation of guard cell anion channels by the protein kinase OPEN STO

64 sumption and stress tolerance by controlling guard cell aperture and other protective responses.

65 the molecular basis for circadian control of guard cell aperture, we used large-scale qRT-PCR to comp

66 opening, we have generated SGC (specifically guard cell) Arabidopsis (Arabidopsis thaliana) plants in

67 Although it has long been observed that guard cells are anisotropic due to differential thickeni

68 The focl1 guard cells are larger and less able to reduce the apert

69 Stomatal guard cells are pairs of specialized epidermal cells tha

70 Here, we show that the walls of guard cells are rich in un-esterified pectins.

71 Stomatal guard cells are widely recognized as the premier plant c

72 orms the basis of using the size of stomatal guard cells as a proxy to track changes in plant genome

73 acylglycerols (TAGs), present in Arabidopsis guard cells as lipid droplets (LDs), are involved in lig

74 dest alteration of Ca transient frequency in guard cells, associated with the absence of Ca-induced s

75 Starch is present in guard cells at the end of night, unlike in the rest of t

76 ether pore formation is an outcome of sister guard cells being pulled away from each other upon turgo

77 Mal inhibited the anion current of Kalanchoe guard cells, both in wild-type and RNAi mutants with imp

78 e [ADGase]) or retain starch accumulation in guard cells but are starch deficient in mesophyll cells

79 did not, showing that starch biosynthesis in guard cells but not mesophyll functions in CO2-induced s

80 rd type of CPK, CPK13, which is expressed in guard cells but whose role is still unknown.

81 The accumulation of flavonol antioxidants in guard cells, but not surrounding pavement cells, was vis

82 els of flavonols are positively regulated in guard cells by ethylene treatment in the wild type, but

83 diating a massive K(+) efflux in Arabidopsis guard cells by the phosphatase AtPP2CA was investigated.

84 ic Gbeta subunit, AGB1, is required for four guard cell Ca(o) responses: induction of stomatal closur

85 blished that the circadian oscillator within guard cells can contribute to long-term WUE.

86 extracellular ATP and of leaf mesophyll and guard cell chloroplasts during light-to-low-intensity bl

87 These data suggest that guard cell CO2 and ABA signal transduction are not direc

88 bitors and suggest a mechanism through which guard cell CO2 signaling controls plant water management

89 H LEAF TEMPERATURE 1 (HT1)-a central node in guard cell CO2 signaling-and that MPK12 functions as an

90 Guard cells collapse inwardly, increase in surface area,

91 pare circadian oscillator gene expression in guard cells compared with the "average" whole-leaf oscil

92 ts and on the pairwise relationships between guard cell components.

93 This process is mainly regulated by guard cell control of the stomatal aperture, but recent

94 hat has been implicated in anionic flux from guard cells controlling the stomatal aperture.

95 The rapidity of gs in dumbbell-shaped guard cells could be attributed to size, whilst in ellip

96 Guard cells determine stomatal aperture and must operate

97 utside inwardly and continues to do so after guard cells die and collapse.

98 tomic force microscopy, that although mature guard cells display a radial gradient of stiffness, this

99 Guard cells dynamically adjust their shape in order to r

100 The stomata, formed by a pair of guard cells, dynamically increase and decrease their vol

101 tants to explore the impact of clustering on guard cell dynamics, gas exchange, and ion transport of

102 ulose and xyloglucan are required for normal guard cell dynamics.

103 ls and stomatal responses require reversible guard cell elongation and contraction.

104 mechanical, pectin-based pinning down of the guard cell ends, which restricts increase of stomatal co

105 s well as diel changes in their abundance in guard cell-enriched epidermis and mesophyll cells from l

106 nstrated that ABA induces DES1 expression in guard cell-enriched RNA extracts from wild-type Arabidop

107 High temperature promotes guard cell expansion, which opens stomatal pores to faci

108 In silico analysis revealed that guard cells express all the genes required for beta-oxid

109 matal aperture through its inhibition of the guard cell-expressed KAT2 and KAT1 channels.

110 We combine several approaches to identify a guard cell-expressed target.

111 We have characterized FOCL1, a guard cell-expressed, secreted protein with homology to

112 In plant guard cells, extracellular calcium (Ca(o) ) is as strong

113 ntified 390 distinct metabolites in B. napus guard cells, falling into diverse classes.

114 ributed to size, whilst in elliptical-shaped guard cells features other than anatomy were more import

115 b1 and Glb2 scavenge NO produced in stomatal guard cells following ABA supply; plants overexpressing

116 meeting these challenges and to engineering guard cells for improved water use efficiency and agricu

117 Guard cells form stomatal pores that optimize photosynth

118 appear unchanged at the transcript level in guard cells from C3 and C4 species, but major variations

119 ne Ontology terms previously associated with guard cells from the C3 model Arabidopsis (Arabidopsis t

120 digesting enzymes, coupled with bioassay of guard cell function) plus modeling lead us to propose th

121 highlight the role of polar reinforcement in guard cell function, which simultaneously improves our u

122 electrophysiological activities required for guard cell function.

123 Guard cells (GCs) display transcriptional memory that is

124 ary cells (SCs) flanking two dumbbell-shaped guard cells (GCs)-is linked to improved stomatal physiol

125 The regulation of guard cell genes involved in controlling stomatal moveme

126 We show that a range of core guard cell genes, including SPCH/MUTE, SMF, and FAMA, ma

127 cal link between OsGRXS17, the modulation of guard cell H2O2 concentrations, and stomatal closure, ex

128 After the rapid H(+) efflux, the Col-0 guard cells had a longer oscillation period than before

129 pme6-1 mutant guard cells have walls enriched in methyl-esterified pec

130 time-dependent outward potassium currents in guard cells, higher rates of water loss through transpir

131 ]i oscillations and analyze their origins in guard cell homeostasis and membrane transport.

132 tool with which to explore the links between guard cell homeostasis, stomatal dynamics, and foliar tr

133 to plants increased flavonol accumulation in guard cells; however, no flavonol increases were observe

134 nel by the protein kinases OPEN STOMATA1 and GUARD CELL HYDROGEN PEROXIDE-RESISTANT1 (GHR1) in Xenopu

135 ctors can utilize intrinsic HDAC activity to guard cell identity by repressing lineage-inappropriate

136 tissue, and that H3K27me3 dynamics regulate guard cell identity.

137 REDUCTASE (NR)-mediated nitric oxide (NO) in guard cells in an abscisic acid (ABA)-independent manner

138 Here, we characterize transcriptomes from guard cells in C3 Tareneya hassleriana and C4 Gynandrops

139 predominantly in the phloem-loading zone and guard cells in leaves, root vasculature, and shoot apica

140 as physiological characteristics of stomatal guard cells in order to accelerate stomatal movements in

141 ed [Ca(2+)]cyt oscillations in epidermal and guard cells in response to the fungal elicitor chitin.

142 Decreased flavonols in guard cells in the anthocyanin reduced (are) mutant and

143 ion across the bundle sheath, mesophyll, and guard cells in the C4 leaf.

144 to the characteristic patterning of stomatal guard cells in the context of a growing leaf.

145 The position of guard cells in the epidermis is ideally suited for cellu

146 d an elevation in H2O2 production within the guard cells, increased sensitivity to ABA, and a reducti

147 Further, upon initiation of reprogramming, guard cells induce H3K27me3-mediated repression of a reg

148 The shape of the guard cells influenced the rapidity of response and the

149 it, the capacity for lateral displacement of guard cells into neighboring epidermal cells, are crucia

150 e found that irreversible differentiation of guard cells involves RETINOBLASTOMA-RELATED (RBR) recrui

151 d phenotype, suggests that photosynthesis in guard cells is critical for energization and guard cell

152 mponent of phototropin signaling in stomatal guard cells is discussed.

153 ledge on CO2 signal transduction in stomatal guard cells is limited.

154 take or release of ions and metabolites from guard cells is necessary to achieve normal stomatal func

155 l responsible for the release of malate from guard cells, is essential for efficient stomatal closure

156 hesis and signalling with K(+) nutrition and guard cell K(+) channel activities have not been fully e

157 ing potassium (K(+) ) nutrition and a robust guard cell K(+) inward channel activity is considered cr

158 e xyloglucan, stomatal apertures, changes in guard cell length, and cellulose reorganization were abe

159 scale investigation into changes in stomatal guard-cell length and use these data to infer changes in

160 ease in ABA biosynthesis specifically in the guard cell lineage.

161 leaves, suggesting that the SA signaling in guard cells may be independent from other cell types.

162 s, promotes rosette expansion, and modulates guard cell mechanics in adult plants.

163 y, CO2, and light, but without connection to guard cell mechanics.

164 e main inward rectifying channels present in guard cells, mediating K(+) influx into these cells, res

165 Guard cell membrane transport is integral to controlling

166 profiling and identification of Arabidopsis guard cell metabolic signatures in response to red light

167 ly well understood, whereas our knowledge of guard cell metabolism remains limited, despite several d

168 ints to multiple processes and plasticity in guard cell metabolism that enable these cells to functio

169 rther exploring and potentially manipulating guard cell metabolism to improve plant water use and pro

170 eview some of the osmoregulatory pathways in guard cell metabolism, genes and signals that determine

171 Therefore, analysis of guard cell metabolites is fundamental for elucidation of

172 The red-light-modulated guard cell metabolome reported here provides fundamental

173 indings reveal that high temperature-induced guard cell movement requires components involved in blue

174 dy increases while the moderate increases in guard cell nuclear size did not justify for a ploidy inc

175 Flavonols accumulate in guard cells of Arabidopsis thaliana, but not surrounding

176 Guard cells of are show greater ABA-induced closure than

177 photosynthesis were more highly expressed in guard cells of C4 compared with C3 leaves.

178 +) (K(+) (in) ) channels was impaired in the guard cells of cipk23 mutants, whereas activation of the

179 quantitative analysis of starch turnover in guard cells of intact leaves during the diurnal cycle.

180 Reductions in LD abundance in guard cells of the lycophyte Selaginella suggest that TA

181 dants, higher levels of ROS were detected in guard cells of the tomato are mutant and lower levels we

182 Guard cells of transparent testa4-2 show more rapid ABA-

183 e detected using a fluorescent ROS sensor in guard cells of transparent testa4-2, which has a null mu

184 rane ion fluxes of H(+) , Ca(2+) and K(+) in guard cells of wild-type (Col-0) Arabidopsis, the CORONA

185 sis plants that are chlorophyll-deficient in guard cells only, expressing a constitutively active chl

186 than the wild type, reduced light-dependent guard cell opening, and reduced water loss, with aw havi

187 plants where ABA biosynthesis was rescued in guard cells or phloem companion cells of an ABA-deficien

188 sing genetic approaches, we show that ABA in guard cells or their precursors is sufficient to mediate

189 Moreover, the differences in guard cell oscillator function may be important for the

190 misexpressed CCA1 Our results show that the guard cell oscillator is different from the average plan

191 onsistent with a disabled active response of guard cell osmotic pressure.

192 FOCL1-GFP localizes to the guard cell outer cuticular ledge and plants lacking FOCL

193 The regulation of the GORK (Guard Cell Outward Rectifying) Shaker channel mediating

194 Arabidopsis plants overexpressing circGORK (Guard cell outward-rectifying K(+) -channel) were hypers

195 ata and developed a biomechanical model of a guard cell pair.

196 uate the current literature on metabolism in guard cells, particularly the roles of starch, sucrose,

197 ctance measurements using intact plants, and guard cell patch-clamp experiments were performed.

198 red stomatal closure requires an increase in guard cell permeability to water and possibly hydrogen p

199 While the function of mesophyll cells, guard cells, phloem companion cells and sieve elements a

200 The role of guard cell photosynthesis in stomatal conductance respon

201 e the possible origins of sucrose, including guard cell photosynthesis, and discuss new evidence that

202 l transduction are not directly modulated by guard cell photosynthesis/electron transport.

203 epted that differential radial thickening of guard cells plays an important role in the turgor-driven

204 dence that HG delivery and modification, and guard cell pressurization, make functional contributions

205 In this study, Brassica napus guard-cell proteins altered by redox in response to absc

206 crease in osmotic water permeability (Pf) of guard cell protoplasts and an accumulation of reactive o

207 of PIP2;1 constitutively enhanced the Pf of guard cell protoplasts while suppressing its ABA-depende

208 ic signatures in response to ABA in B. napus guard cell protoplasts.

209 nase inhibition decreases FRET efficiency in guard cells, providing direct experimental evidence that

210 OnGuard software and models of the stomatal guard cell recently developed for exploring stomatal phy

211 layers to the increasingly complex system of guard cell regulation.

212 chanism underlying CO(2) sensing in stomatal guard cells remains unclear.

213 ins were identified in ABA- and MeJA-treated guard cells, respectively.

214 of protoplasts, firmly establishing a direct guard cell response to red light.

215 Imaging cellulose organization in guard cells revealed a relatively uniform distribution o

216 Activation of the guard cell S-type anion channel SLAC1 is important for s

217 -induced stomatal opening arises from direct guard cell sensing of red light versus indirect response

218 re reject cycadalean affinities, whereas its guard cell shape and stomatal ledges are angiospermous.

219 Guard cells shrink and close stomatal pores when air hum

220 e constructed a multi-level dynamic model of guard cell signal transduction during light-induced stom

221 ew functional role of small GTPase, NOG1, in guard cell signaling and early plant defense in response

222 evidence that the regulatory patterns of key guard cell signaling genes are linked with the character

223 Specially, NOG1-2 regulates guard cell signaling in response to biotic and abiotic s

224 etabolites is fundamental for elucidation of guard cell signaling pathways.

225 posttranslational modifications to fine-tune guard cell signaling.

226 Although the guard-cell-signaling pathway coupling blue light percept

227 e signalling pathways of abiotic stress, but guard cell signalling in response to microbes is a relat

228 t of histidine phosphotransferases (AHPs) in guard cell signalling remain to be fully elucidated.

229 A lack of correlation between guard cell size and DNA content, lack of arabinans in ce

230 ed with trichome branch number increases and guard cell size increases, respectively.

231 Evidence from fossil guard cell size suggests that polyploidy in Sequoia date

232 a constitutively active chlorophyllase in a guard cell specific enhancer trap line.

233 most likely autonomous pools: a constitutive guard cell-specific pool and a facultative environmental

234 Expressing S-della under the control of a guard-cell-specific promoter was sufficient to increase

235 ) was overexpressed under the control of the guard-cell-specific promoter, GC1.

236 Our results show that guard cell starch degradation has an important role in p

237 cs to define the mechanism and regulation of guard cell starch metabolism, showing it to be mediated

238 Diel rescheduling of guard cell starch turnover in K. fedtschenkoi compared w

239 In guard cells, starch is rapidly mobilized by the synergis

240 onsistent with its known daytime role in the guard cell stroma.

241 , this ion transport was abolished in coi1-1 guard cells, suggesting that MeJA-induced transmembrane

242 Stomatal guard cells surround pores in the epidermis of plant lea

243 ynthetic, light-gated K(+) channel BLINK1 in guard cells surrounding stomatal pores in Arabidopsis to

244 olute accumulation by, and its loss from the guard cells surrounding the pore.

245 he regulatory networks and ion fluxes in the guard cells surrounding the stomatal pore [2].

246 but not JA-dependent response, is faster in guard cells than in whole leaves, suggesting that the SA

247 across the plasma and vacuolar membranes of guard cells that drive stomatal movements and the signal

248 enhanced disease susceptibility 1 (EDS1), in guard cells that form stomata.

249 Plant gas exchange is regulated by guard cells that form stomatal pores.

250 tails of the local separation between sister guard cells that give rise to the stomatal pore or how f

251 esponses to [CO2 ] and ABA are functional in guard cells that lack chlorophyll.

252 Stomata are defined by pairs of guard cells that perceive and transduce external signals

253 Each stomate is bordered by a pair of guard cells that shrink in response to drought and the a

254 Plant guard cells, that form stomatal pores for gas exchange,

255 n the epidermal cells of the root tip and in guard cells, the latter of which regulate the size of st

256 Here, we report that, in Arabidopsis guard cells, the tonoplast-localized K(+)/H(+) exchanger

257 other parts of the leaf rather than from the guard cells themselves.

258 transport, metabolism, and signaling of the guard cell to define the water relations and transpirati

259 on changes in turgor pressure acting within guard cells to alter cell shape [1].

260 ) increases reactive oxygen species (ROS) in guard cells to close Arabidopsis (Arabidopsis thaliana)

261 port metabolomic responses of Brassica napus guard cells to elevated CO2 using three hyphenated metab

262 negative regulator of GA signaling, acts in guard cells to promote stomatal closure and reduce water

263 bryophyte monophyly and demonstrate that the guard cell toolkit is more ancient than has been appreci

264 no effect on the induction of heat-sensitive guard cell transcripts, supporting the existence of an a

265 We conclude that manipulating guard cell transport and metabolism is just as, if not m

266 by a dynamic, mechanistic model that assumes guard cell turgor changes in concert with leaf turgor in

267 s, and plasma membrane channels that control guard cell turgor pressure.

268 guard cells is critical for energization and guard cell turgor production.

269 n in the proteins responsible for regulating guard cell turgor.

270 Our results show that guard cell vacuolar accumulation of K(+) is a requiremen

271 w that Wortmannin also induced the fusion of guard cell vacuoles in fava beans, where vacuoles are na

272 ends on changes in osmolyte concentration of guard cell vacuoles, specifically of K(+) and Mal(2-) Ef

273 The importance of ABA biosynthesis in guard cells versus vasculature for whole-plant stomatal

274 tic solutes that drive reversible changes in guard cell volume and turgor.

275 re governed by osmotically driven changes in guard cell volume, the role of membrane water channels (

276 Combined experimental data (analysis of guard cell wall epitopes and treatment of tissue with ce

277 Hence, PME34 is required for regulating guard cell wall flexibility to mediate the heat response

278 Restoration of PME6 rescues guard cell wall pectin methyl-esterification status, sto

279 function reflects a mechanical change in the guard cell wall.

280 these results provide new insights into how guard cell walls allow stomata to function as responsive

281 ological and genetic analyses to investigate guard cell walls and their relationship to stomatal func

282 xible, but how the structure and dynamics of guard cell walls enable stomatal function remains poorly

283 chanisms for how stomatal pores form and how guard cell walls facilitate dynamic stomatal responses r

284 e signals suggesting that the flexibility of guard cell walls is impaired.

285 at are driven by changes in turgor pressure, guard cell walls must be both strong and flexible, but h

286 , the H(+) efflux and Ca(2+) influx in Col-0 guard cells was impaired by vanadate pre-treatment or PM

287 inating from the appressoria formed over the guard cells, was thought to require light to induce natu

288 Our data show that more than 90% of guard cells were chlorophyll-deficient.

289 A total of 358 metabolites from guard cells were quantified in a time-course response to

290 getative plants, BAM1 acts during the day in guard cells, whereas BAM3 is the dominant activity in me

291 ed in actin-dependent nuclear positioning in guard cells, whereas its paralogue SINE2 contributes to

292 Stomata are formed by a pair of guard cells which have thickened, elastic cell walls to

293 Guard cells, which flank the stomata, undergo adjustment

294 through the development of a new cell type: guard cells, which form stomata.

295 ith the turgor pressure of the epidermis and guard cells, which ultimately determine stomatal pore si

296 the expression of other transporter genes in guard cells, which ultimately led to improved growth.

297 n accumulation of reactive oxygen species in guard cells, which were both abrogated in pip2;1 plants.

298 division to differentiate highly specialized guard cells while maintaining a stem cell population [1,

299 We quantified 223 metabolites in Arabidopsis guard cells, with 104 found to be red light responsive.

300 f stiffness, this is not present in immature guard cells, yet young stomata show a normal opening res