ライフサイエンスコーパス: gustducin

1 icient in the gustducin alpha-subunit (alpha-gustducin).

2 mmunoreactivity to an antibody against alpha-gustducin.

3 a bitter-responsive receptor that activates gustducin.

4 racellular facet, bridging the receptor with gustducin.

5 s and the tastant-associated G protein alpha-gustducin.

6 sucralose internalized T1R2, T1R3 and alpha-gustducin.

7 ptor antagonist lactisole or siRNA for alpha-gustducin.

8 ngual taste cells and strongly express alpha-gustducin.

9 t not in knockout mice lacking T1R3 or alpha-gustducin.

10 Mouse intestinal L cells also express alpha-gustducin.

11 ussis toxin and by genetic deletion of alpha-gustducin.

12 all, bitter-responsive cells expressed alpha-gustducin.

13 (SNAP-25), and to a lesser extent with alpha-gustducin.

14 reduced by 70% in mutant mice lacking alpha-gustducin.

15 ing the molecular markers Vimentin and alpha-Gustducin.

16 antigen, whether or not they expressed alpha-gustducin.

17 ate specific tastant-dependent activation of gustducin, a G protein implicated in bitter signaling.

18 press T2R "bitter-taste" receptors and alpha-gustducin, a G protein involved in chemosensory transduc

19 subset of type II cells that contains alpha-gustducin, a G-protein involved in bitter transduction,

20 butions of these antigens with that of alpha-gustducin, a G-protein subunit implicated in responses t

21 A key molecule, alpha-gustducin, a primarily taste-specific G protein alpha-su

22 Alpha-gustducin, a taste cell-expressed G-protein alpha subuni

23 Gustducin, a taste-specific G-protein closely related to

24 Gustducin, a transducin-like guanine nucleotide-binding

25 eins derived from visual transducin or taste gustducin alpha subunits, but no other Galpha HD protein

26 ing and characterizing mice deficient in the gustducin alpha-subunit (alpha-gustducin).

27 weet and amino acid taste receptors, and the gustducin alpha-subunit GNAT3 leads to male-specific ste

28 The alpha subunit of gustducin (alpha-gustducin) is critical for transduction

29 Signaling components included gustducin-alpha and Galphao, but not rod or cone transdu

30 Gustducin-alpha resembles transducin-alpha functionally

31 We set out to determine whether alpha-gustducin also mediates umami taste and whether rod alph

32 Umami detection involving alpha-gustducin and alpha(t-rod) occurs in anteriorly placed t

33 Both alpha-gustducin and alpha-transducin activate phosphodiesteras

34 generated a dominant-negative form of alpha-gustducin and expressed it as a transgene from the alpha

35 Both Galpha gustducin and Galpha transducin are involved in umami si

36 entiated light cells that also express alpha-gustducin and may be involved in intercellular interacti

37 onical signaling components (i.e., G-protein gustducin and phospholipase C beta2).

38 nds on an interaction with the C terminus of gustducin and requires G-protein betagamma subunits to p

39 Gustducin and rod transducin, which is also expressed in

40 were incubated with antibodies against alpha-gustducin and the human blood group A antigen.

41 Thus, proposed models for alpha-gustducin and those found by other laboratories may be p

42 east two varieties: those immunoreactive for gustducin and those immunoreactive for PGP 9.5.

43 Gustducin and transducin are activated in the presence o

44 pressed in bovine taste tissue and that both gustducin and transducin, in the presence of bovine tast

45 eptors are coupled through G-proteins, alpha-gustducin and transducin, to activate phospholipase C be

46 taste transduction cascade involving Galpha-gustducin and transient receptor potential melastatin 5.

47 s of single and double KO mice lacking alpha-gustducin and/or alpha(t-rod) confirmed the involvement

48 AS2R14 complexed with G(gust) (also known as gustducin) and G(i1).

49 AS2R14 in complex with its signaling partner gustducin, and bound to flufenamic acid (FFA), a clinica

50 a2 (PLCbeta2) or the G-protein subunit alpha-gustducin, and serotonin (5HT) as markers of type I, II,

51 s sweet taste receptors, the taste G protein gustducin, and several other taste transduction elements

52 s sweet taste receptors, the taste G protein gustducin, and several other taste transduction elements

53 Both the gustatory G-protein, alpha-gustducin, and the cell-surface carbohydrate, the A bloo

54 nd their associated heterotrimeric G protein gustducin are involved in sugar and amino acid sensing i

55 al and genetic studies have implicated alpha-gustducin as a key component in the transduction of both

56 l experiments, expression of wild-type alpha-gustducin as a transgene in alpha-gustducin-null mice fu

57 All Lewis(b)-positive cells expressed alpha-gustducin, but only a fraction of alpha-gustducin-positi

58 the activation in response to denatonium of gustducin by presumptive bitter-responsive receptors pre

59 tivity should be reflected in the numbers of gustducin-containing cells in different taste bud popula

60 Taste cells with alpha-gustducin could express either presynaptic proteins or t

61 troduced into the C-terminal region of alpha-gustducin critical for receptor interaction rendered the

62 eceptor subunit T1R3 and the taste G protein gustducin, expressed in enteroendocrine cells, underlie

63 ssion was found exclusively in the G-protein gustducin-expressing bitter receptor cells, while TNF wa

64 fungiform taste buds contained twice as many gustducin-expressing cells (6.8/taste bud) as those of t

65 mal vallate papilla had a mean of 8.37 alpha-gustducin-expressing cells and 5.22 A-expressing cells p

66 Unexpectedly, all gustducin-expressing cells lacked voltage-gated Ca(2+) c

67 w-affinity sweet taste receptor, stimulating gustducin G protein (G(Gust)) signaling in the presence

68 t family considered to be a component of the gustducin G-protein heterotrimer involved in bitter and

69 cin Galpha (Galphat) and the closely related gustducin (Galphag), but not Galphai1, Galphas, or Galph

70 Gustducin heterotrimers (alpha-gustducin/Gbeta1/Ggamma13) were activated by taste cell

71 from the upstream region of the mouse alpha-gustducin gene acts as a fully functional promoter to ta

72 ving that the targeted deletion of the alpha-gustducin gene caused the taste deficits of the null mic

73 h "knock-out" animals deficient in the alpha-gustducin gene clearly demonstrate that gustducin is an

74 hancer from the distal portion of the murine gustducin gene that, in combination with the minimal pro

75 with taste-signaling molecules such as alpha-gustducin, Ggamma13, phospholipase C-beta2 (PLC-beta2) a

76 Of 19 alpha-gustducin/Ggamma13-positive taste receptor cells profile

77 2rs and the taste signaling components alpha-gustducin (Gnat3), TrpM5, and Plcbeta2.

78 Gustducin heterotrimers (alpha-gustducin/Gbeta1/Ggamma13

79 We conclude that gustducin heterotrimers transduce responses to bitter an

80 Although gustducin-immunoreactive taste cells appear similar in o

81 imaging in lingual slices and examined alpha-gustducin immunoreactivity in the same cells.

82 The ultrastructural distribution of gustducin immunoreactivity is consistent with its propos

83 lls that contain the G protein alpha subunit gustducin, implying that they function as gustducin-link

84 ha(t-rod) confirmed the involvement of alpha-gustducin in bitter (quinine and denatonium) and sweet (

85 d sweet compounds, the precise role of alpha-gustducin in bitter and sweet taste is presently unclear

86 t involvement of the G-protein subunit alpha-gustducin in bitter taste transduction in taste cells ha

87 was present on many cells that lacked alpha-gustducin in foliate and vallate papillae.

88 mmunoreactivity to antisera directed against gustducin in taste buds of rat circumvallate papilla.

89 NF expression to the postnatal expression of gustducin in taste cells.

90 bunit (Ggamma13) that colocalized with alpha-gustducin in taste receptor cells.

91 on taste-bud cells that also expressed alpha-gustducin in the order: foliate and vallate papillae > n

92 te and demonstrated the involvement of alpha-gustducin in umami [monosodium glutamate (MSG), monopota

93 ng paper, we demonstrate that T2Rs couple to gustducin in vitro, and respond to bitter tastants in a

94 t compounds, and to elicit the nature of the gustducin-independent pathways, we generated a dominant-

95 The taste receptor-gustducin interaction can be competitively inhibited by

96 A proposed mechanism for alpha-gustducin involves coupling specific cell-surface recept

97 Our results suggest that gustducin is a principal mediator of both bitter and swe

98 Gustducin is a taste receptor cell (TRC)-specific G prot

99 Gustducin is a transducin-like G protein (guanine nucleo

100 Gustducin is a transducin-like G protein selectively exp

101 lpha-gustducin gene clearly demonstrate that gustducin is an essential molecule for tasting certain b

102 Gustducin is believed to be involved in bitter and possi

103 In this paper, we show that gustducin is expressed in bovine taste tissue and that b

104 These data support the hypothesis that alpha-gustducin is involved in the transduction of both sweet-

105 The alpha subunit of gustducin (alpha-gustducin) is critical for transduction of responses to

106 Alpha-gustducin knock-out (KO) mice have greatly diminished, b

107 ioral sensitivity to bitter stimuli in alpha-gustducin knock-out mice thus appears to be the conseque

108 and this prevention is largely lost in alpha-gustducin-knockout mice.

109 of the taste responses that remain in alpha-gustducin KO mice.

110 g regulatory proteins expressed in the alpha-gustducin lineage of taste cells mediate these responses

111 it gustducin, implying that they function as gustducin-linked receptors.

112 cted results with knockout mice suggest that gustducin may be directly involved in both bitter and sw

113 To gain insights into how gustducin mediates responses to bitter and sweet compoun

114 The gustducin minimal 1.4 kb promoter (GUS(1.4)) by itself w

115 patterns of GAD with the TRC protein markers gustducin, neural cell adhesion molecule, protein gene p

116 hape to the pyriform PGP 9.5/NSE population, gustducin never colocalizes with PGP 9.5 or NSE.

117 ed small intestine and intestinal villi from gustducin null mice displayed markedly defective glucago

118 Ingestion of glucose by alpha-gustducin null mice revealed deficiencies in secretion o

119 small bowel and intestinal villi from alpha-gustducin null mice showed markedly defective GLP-1 secr

120 t alpha-gustducin restored responsiveness of gustducin null mice to both bitter and sweet compounds,

121 Yet, alpha-gustducin-null mice are not completely unresponsive to b

122 type alpha-gustducin as a transgene in alpha-gustducin-null mice fully restored responsiveness to bit

123 and sweet taste responsiveness of the alpha-gustducin-null mice suggests that other guanine nucleoti

124 stducin promoter in both wild-type and alpha-gustducin-null mice.

125 ivity for the taste cell-specific G protein, gustducin, occurs in a subset ofrENaC positive taste cel

126 as a normal number of cells expressing alpha-gustducin or the A antigen in regenerated taste buds; in

127 Knockout mice that lack gustducin or the sweet taste receptor subunit T1r3 have

128 There was no evidence for the activation of gustducin or transducin in the beta-cell.

129 Genetic ablation of either G alpha-gustducin or TrpM5, essential elements of the T2R transd

130 a common second-messenger cascade involving gustducin, phospholipase C beta2, and the transient rece

131 bitter taste signaling cascade (i.e., TAS2R-gustducin-phospholipase Cbeta [PLCbeta]- inositol 1,4,5-

132 ste buds of the fungiform papillae had fewer gustducin-positive cells (3.1/taste bud) than those of o

133 lpha-gustducin, but only a fraction of alpha-gustducin-positive cells expressed Lewis(b).

134 Gustducin-positive cells were seen in all taste bud regi

135 r to target lacZ transgene expression to the gustducin-positive subset of taste receptor cells (TRCs)

136 transgene expression was driven by an alpha-gustducin promoter coupling BDNF expression to the postn

137 d expressed it as a transgene from the alpha-gustducin promoter in both wild-type and alpha-gustducin

138 compounds, demonstrating the utility of the gustducin promoter.

139 fluorescent protein under the control of the gustducin promoter.

140 Nevertheless, some taste cells lacking alpha-gustducin responded to bitter stimuli, suggesting that o

141 Transgenic expression of rat alpha-gustducin restored responsiveness of gustducin null mice

142 mutant transgene inhibited endogenous alpha-gustducin's interactions with taste receptors, i.e., it

143 nses to bitter and sweet compounds via alpha-gustducin's regulation of phosphodiesterase (PDE) and Gb

144 Here we investigate gustducin's role in taste transduction by generating and

145 rs neuronal cell adhesion molecule and alpha-gustducin, suggesting that both the taste receptor cells

146 a2+ channels, K(+)ATP channels and the alpha-gustducin taste pathway do not appear to be involved.

147 e human L cell line NCI-H716 expresses alpha-gustducin, taste receptors, and several other taste sign

148 bitter taste, apparently unrelated to alpha-gustducin, that increase cyclic AMP or inositol 1,4,5 tr

149 eteners, which act through T1R2 + T1R3/alpha-gustducin to activate PLC beta2 and PKC betaII.

150 e colocalized with each other and with alpha-gustducin, transducin or phospholipase C beta2 to differ

151 ssbred with green fluorescent protein (GFP) (gustducin) transgenic mice.

152 eciprocal regulation of T1R2, T1R3 and alpha-gustducin versus T1R1, transducin and phospholipase C be

153 from the GUS(8.4) promoter and of endogenous gustducin was coordinately lost after nerve section and

154 d with antibodies against Vimentin and alpha-Gustducin were easily identified and counted under a lig

155 The G-protein subunit alpha-gustducin, which is similar to rod transducin, has been