ライフサイエンスコーパス: hen egg lysozyme

1 odominance and local structural stability in hen egg lysozyme.

2 LPS inhibited processing of bovine RNase and hen egg lysozyme.

3 following exposure of the cells to exogenous hen egg lysozyme.

4 e in model autoantibody systems such as anti-hen egg lysozyme.

5 y affinity mutants of the model antigen (Ag) hen egg lysozyme.

6 transgenic CD4 murine cells specific against hen egg lysozyme.

7 o antigen, E/HEL-Tg mice were immunized with hen egg lysozyme.

8 migrated to T cell areas in the response to hen egg lysozyme.

9 xhibit exaggerated tolerance to the model Ag hen-egg lysozyme.

10 In vivo, a low dose of hen egg lysozyme (1 microg) induced significant CD83 but

11 utamic acid decarboxylase 524-543 (self) and hen egg lysozyme 11-23 (foreign) I-A(g7)-binding peptide

12 boxylase (GAD) 206-220 or GAD 524-538 or for hen egg lysozyme 11-25 as a control in NOD, NOD-scid, an

13 In contrast, 3A9 T cells (specific for hen egg lysozyme(46-61)/I-A(k)) are completely eliminate

14 ells, whereas a VLR:Tmu receptor specific to hen egg lysozyme (a self-antigen with respect to chicken

15 The response to hen egg lysozyme, a model antigen with a compact structu

16 ozyme and expressing chronically circulating hen egg lysozyme Ag resulted in induction of high and su

17 pe and CD72-deficient B cells in response to hen egg lysozyme Ag stimulation.

18 mounts of rHEL-C3d3 were more effective than hen egg lysozyme alone in up-regulating c-FLIP levels an

19 ied by functionalization with biotin-labeled hen egg lysozyme amyloid fibers.

20 ss a chimeric membrane protein consisting of hen egg lysozyme and a hemoglobin epitope tag under the

21 sma VLRB responses of lamprey immunized with hen egg lysozyme and beta-galactosidase (beta-gal), demo

22 Site-specific photocleavage of hen egg lysozyme and bovine serum albumin (BSA) by N-(l-

23 rearranged B cell Ag receptors specific for hen egg lysozyme and expressing chronically circulating

24 protein that binds the acidic active site of hen egg lysozyme and inhibits the enzyme.

25 terminus of beta-catenin, the model proteins hen egg lysozyme and streptavidin, and immunoglobulins f

26 The crystal structure of the complex between hen egg lysozyme and the Fv fragment of a humanized anti

27 were crossed with mice that express soluble hen egg lysozyme and whose B cells bear receptors specif

28 not limited to Smith Ag-specific B cells as hen egg lysozyme- and p-azophenylarsonate-specific B cel

29 mature, respond to antigen, and secrete anti-hen egg lysozyme antibodies.

30 e B10.BR mice transfused with membrane-bound hen egg lysozyme antigen-transgenic RBCs also demonstrat

31 in which naive transgenic cells specific to hen egg lysozyme are adoptively transferred into recipie

32 Equilibrium unfolding of hen egg lysozyme as a function of urea concentration at

33 examine the performance of this method with hen egg lysozyme as a model system, reproducing its well

34 and timing of exposure to different forms of hen egg lysozyme as a self-Ag.

35 igh and sustained levels of circulating anti-hen egg lysozyme autoantibodies and glomerulonephritis w

36 Anergic hen egg lysozyme autoantigen-binding B cells are also sh

37 by exposing female C57BL/6 wild-type mice or hen egg lysozyme B-cell receptor transgenic mice to desi

38 an anti-ovalbumin T-cell receptor or an anti-hen egg lysozyme B-cell receptor were studied.

39 pansion of Tregs did not occur in BAFF-Tg/Ig hen egg lysozyme BCR chimeras, demonstrating a requireme

40 splenic B lymphocytes from wild-type or anti-hen egg lysozyme BCR transgenic mice, and on a mature mo

41 c models of B cell self-reactivity, the anti-hen egg lysozyme BCR transgenic strain and the AM14 rheu

42 sgenic mice, "edited" B cells that carry non-hen egg lysozyme binding receptors preferentially accumu

43 After immunization with soluble hen egg lysozyme, both MZ and FO B cells captured Ag and

44 vaccinia virus (a smallpox virus surrogate), hen egg lysozyme, cholera toxin, ricin, and staphylococc

45 approached by a bi-specific antibody against hen egg lysozyme consisting of scFv fragments of D1.3 an

46 Finally, by using hen egg lysozyme double transgenic mice, we demonstrated

47 Using the Ig and hen egg lysozyme double transgenic mouse model, we demon

48 Using a hen egg lysozyme double-transgenic model, we further dem

49 s expressed in B cells that are specific for hen egg lysozyme (E/HEL-Tg).

50 3, which flank the immunodominant epitope of hen egg lysozyme (HEL 52-61), were shown to have a profo

51 plex with a naturally processed peptide from hen egg lysozyme (HEL residues 50-62) at 1.9 A resolutio

52 A mouse model utilizing hen egg lysozyme (HEL) "anergic" B cells was studied.

53 Using an mAb (C4H3) specific for the hen egg lysozyme (HEL) 46-61 determinant bound to I-Ak,

54 PFR of the H-2A(k) immunodominant epitope of hen egg lysozyme (HEL) 52-61.

55 active B cells expressing high affinity anti-hen egg lysozyme (HEL) Ag receptors exposed in vivo to m

56 mice that are double-transgenic for soluble hen egg lysozyme (HEL) and an Ig that recognizes HEL wit

57 entation, using a well-characterized antigen hen egg lysozyme (HEL) and comparing it with the closely

58 fic mAb reactive with these structures using hen egg lysozyme (HEL) and I-Ak as a model system.

59 nic (Tg) mice that express a soluble form of hen egg lysozyme (HEL) and in which B cell tolerance to

60 re compared for four antigenic epitopes from hen egg lysozyme (HEL) and RNase.

61 s a transgenic antigen receptor specific for hen egg lysozyme (HEL) and that either lack functional F

62 the association of the 46-61 determinant of hen egg lysozyme (HEL) and the mouse MHC class II molecu

63 in which TCR-transgenic Th cells specific to hen egg lysozyme (HEL) are adoptively transferred to rec

64 4 cells from TCR transgenic mice specific to hen egg lysozyme (HEL) are polarized with IL-6/TGF-beta

65 Cs from transgenic mHEL mice express surface hen egg lysozyme (HEL) as a transmembrane protein.

66 In mice with a diverse B cell repertoire, hen egg lysozyme (HEL) autoantigen-binding B cells are e

67 duction in response to low levels of soluble hen egg lysozyme (HEL) both in vivo and in vitro as dete

68 dy that recognizes peptide residues 48-62 of hen egg lysozyme (HEL) bound to the MHC class II molecul

69 that SIC binds to SLPI and to both human and hen egg lysozyme (HEL) but not to lactoferrin.

70 that B cells, which express BCRs specific to hen egg lysozyme (HEL) display diminished responsiveness

71 shown that fusing complement fragment C3d to hen egg lysozyme (HEL) enhanced the immunogenicity of HE

72 The complex of HyHEL-5 with hen egg lysozyme (HEL) features salt bridges between Fab

73 e observed when crossing STAT5b-CA mice with hen egg lysozyme (HEL) H chain transgenic mice.

74 Mice expressing the transgene (Tg) hen egg lysozyme (HEL) in the retina crossed with 3A9 mi

75 induced in transgenic (Tg) mice that express hen egg lysozyme (HEL) in their lens, by adoptively tran

76 s genome by inserting the p46-63 sequence of hen egg lysozyme (HEL) into the neuraminidase stalk of t

77 of osmolytes on the association of the anti-hen egg lysozyme (HEL) monoclonal antibody HyHEL-5 with

78 ND CD4+ TCR transgenic mice specific for the hen egg lysozyme (HEL) peptide 48-62:I-Ak and moth cytoc

79 ntitated the major families of peptides from hen egg lysozyme (HEL) presented by MHC class II I-A(k)

80 In vivo experiments in hen egg lysozyme (HEL) T cell receptor transgenic mice s

81 Cs to CpG ODN for 48 h blocked processing of hen egg lysozyme (HEL) to HEL(48-61):I-A(k) complexes.

82 se interactions we imaged B cells (TgB) from hen egg lysozyme (HEL) transgenic mice and DC pulsed wit

83 lysozyme auto-Ag-specific B cells in Ig(Tg) hen egg lysozyme (HEL) transgenic mice inhabit the splee

84 Breeding of the hen egg lysozyme (HEL) transgenic model for B cell anerg

85 o-self-antigen in transgenic mice expressing hen egg lysozyme (HEL) under a retina-specific promoter.

86 the state of tolerance in Tg mice expressing hen egg lysozyme (HEL) under control of the rhodopsin pr

87 ee lines of transgenic (Tg) mice, expressing hen egg lysozyme (HEL) under the control of the alphaA-c

88 express soluble (S-) or membrane-bound (M-) hen egg lysozyme (HEL) under the control of the alphaA-c

89 receptor (TCR) transgenic mice specific for hen egg lysozyme (HEL) were crossed with mice expressing

90 m in which naive CD4 cells, specific against hen egg lysozyme (HEL), are injected into recipient mice

91 immunized with a recombinant model antigen, hen egg lysozyme (HEL), fused to murine C3d.

92 ransgenic (Tg) mice expressing a foreign Ag, hen egg lysozyme (HEL), under control of the alphaA-crys

93 ecipient mice immunized with pDNA encoding a hen egg lysozyme (HEL)-IgFc fusion protein (JwHEL)+JwIL4

94 Persistent cross-linking of hen egg lysozyme (HEL)-specific B cell membrane Ig (mIg)

95 reactive B cells, mice doubly transgenic for hen egg lysozyme (HEL)-specific B cell receptors and sol

96 we compared survival and surface markers of hen egg lysozyme (HEL)-specific B cells in Ig transgenic

97 Using primary B cells that express a hen egg lysozyme (HEL)-specific BCR, we found that oligo

98 isplaying a transgene-encoded BCR that binds hen egg lysozyme (HEL).

99 then challenged intratracheally (i.t.) with hen egg lysozyme (HEL).

100 DN on the differentiation of Th responses to hen egg lysozyme (HEL).

101 s II MHC (MHC-II)-restricted presentation of hen egg lysozyme (HEL).

102 rally processed, I-Ak-restricted peptides of hen egg lysozyme (HEL).

103 rying immunoglobulin transgenes specific for hen egg lysozyme (HEL).

104 the model Ags pigeon cytochrome c (PCC) and hen egg lysozyme (HEL).

105 es to Ags containing disulfide bonds such as hen egg lysozyme (HEL).

106 , which express B cell receptor specific for hen egg lysozyme (HEL).

107 -10 interact with nonoverlapping epitopes on hen egg lysozyme (HEL); the HyHEL-5/HEL interface has tw

108 hibitory gene and an Ig receptor recognizing hen egg lysozyme (HEL-Ig) efficiently escaped developmen

109 proteolytic processing of the model antigen hen-egg lysozyme (HEL).

110 ., exposure of CD4 cells to their target Ag, hen egg lysozyme [HEL] and APCs).

111 Using the HOD (hen egg lysozyme [HEL] and ovalbumin [OVA] fused with th

112 ded our findings to another TCR system (anti-hen egg lysozyme [HEL] TCR/HEL mice) where similarly ext

113 usion with RBCs expressing a B-cell epitope (hen egg lysozyme [HEL]) fused to (OVA)(323-339).

114 Allogeneic RBCs expressing the HOD antigen (hen egg lysozyme [HEL]-ovalbumin-human transmembrane Duf

115 ognizing antigens that are (insulin) or not (hen egg lysozyme; HEL) expressed by ss-cells have proven

116 When Clara cell secretory protein-membrane hen egg lysozyme/hemoglobin transgenic mice were crossed

117 Peptide binding studies with variants of the hen egg lysozyme I-Ag7 epitope HEL(11-25) support a comp

118 Here, we demonstrate that anergic B cells in hen egg lysozyme Ig (HEL-Ig)/soluble HEL double transgen

119 Studies in B cell knockout and hen egg lysozyme Ig transgenic mice revealed that B cell

120 since NOD mice immunized with pDNA encoding hen egg lysozyme-IgGFc and IL-4 continued to develop dia

121 nsgenic mice that express a BCR specific for hen egg lysozyme (IgHEL).

122 Hen egg lysozyme immobilized onto polystyrene beads and

123 on (8.4 +/- 1.5 days; n = 18), compared with hen egg lysozyme-immunized C57BL/6 (13.3 +/- 2.2 days; n

124 -)C5ar1(-/-) , and Daf1(-/-) B2 cells and in hen egg lysozyme-immunized muMT recipients of MD4 B2 cel

125 Using OVA and hen egg lysozyme in crisscross fashion, we confirmed the

126 demonstrated that globular proteins, such as hen egg lysozyme in phosphate buffered saline at room te

127 ely transferred into recipients that express hen egg lysozyme in the lens of the eye.

128 adoptively transferred into mice expressing hen egg lysozyme in their eyes, both Th1 and Th17 induce

129 ar inflammation in recipient mice expressing hen egg lysozyme in their eyes.

130 erance of T cells to the transgenic self Ag, hen egg lysozyme, in a strain with a very low serum lyso

131 CR-transgenic Th1 or Th17 cells specific for hen egg lysozyme induce ocular inflammation in recipient

132 polarized Th1 or Th17 cells specific against hen egg lysozyme induce ocular inflammation in recipient

133 ch during B cell ontogeny, autoreactive anti-hen egg lysozyme MD4 Ig transgene B cells are negatively

134 ion, we have studied the development of anti-hen egg lysozyme MD4 Ig transgene B cells while systemat

135 erated selectively expressing membrane-bound hen egg lysozyme (mHEL) on the thyroid epithelium.

136 deamidation of the HEL(48-62) peptide in the hen egg lysozyme model of autoimmunity.

137 ve was incubated with excess protein ligand (hen egg lysozyme or bovine insulin) at 23, 37, or 50 deg

138 ss of stability was accompanied by a loss in hen egg lysozyme or hsp70 peptide-binding ability.

139 ells stimulated via the BCR with the antigen hen egg lysozyme, or surrogate for antigen anti-IgM, dem

140 igen loss, and RBC membrane loss in the HOD (hen egg lysozyme-ovalbumin-human Duffy) murine model.

141 on, neonatal injection of BALB/c mice with a hen egg lysozyme peptide 106-116 in putative "tolergenic

142 ope after immunization with several doses of hen egg lysozyme protein.

143 The main peptides examined were hen egg lysozyme residues 48-62 and heat shock protein (

144 expressing either soluble or membrane-bound hen egg lysozyme (sHEL or mHEL, respectively) under cont

145 mice expressing a model autoantigen (soluble hen egg lysozyme, sHEL) and high-affinity HEL-specific I

146 e tandemly arranged copies of C3d and the Ag hen egg lysozyme, shown to be a highly effective immunog

147 Using hen egg lysozyme-specific (HEL-specific) immunoglobulin

148 To address this question, we used hen egg lysozyme-specific BCR transgenic mice to elucida

149 otoreceptor retinoid-binding promoter, and a hen egg lysozyme-specific CD4(+) TCR transgene.

150 promised neither the formation of functional hen egg lysozyme-specific IgM nor the secretion of free

151 pecific to a different Ag, we tested whether hen egg lysozyme-specific Th1 cells could synergize with

152 at display a cell surface Ag, membrane-bound hen egg lysozyme, strongly activate Ag-specific B cells.

153 e-regulated expression of a secreted form of hen egg lysozyme, tagged with a murine hemoglobin (Hb) e

154 model, we took a segment of polypeptide from hen egg lysozyme that in the native protein forms the bi

155 f principle, we demonstrate this method with hen egg lysozyme that shows at least two kinetic phases

156 Using the hen egg lysozyme transgenic system, we show that IgM(hig

157 ared to 2/26 of saline-treated or to 1/10 of hen egg lysozyme-treated control mice (P < 0.01).

158 generated double-transgenic mice expressing hen egg lysozyme, under the retinal interphotoreceptor r

159 To address these questions, a model Ag, hen egg lysozyme, was targeted to various subcellular co

160 in recipient mice expressing eye-restricted hen egg lysozyme, we found important differences in the

161 trast, two other antigens, phycoerythrin and hen egg lysozyme, were not captured by these cells.

162 he pH-dependent energetics of association of hen egg lysozyme with two closely related monoclonal ant

163 an airway challenge with the soluble antigen hen egg lysozyme yields rapid acquisition of specific an