ライフサイエンスコーパス: histidyl

1 consistent with this compound being 6-(N-1')-histidyl-2-(3, 4-dihydroxyphenyl)ethanol [6-(N-1')-His-D

2 Elements for forming histidyl adenylate and aminoacylation are lacking, sugge

3 In this paper, we investigate the effects of histidyl amino acid modification on high-affinity Mn bin

4 roviding a critical identity element for the histidyl aminoacyl tRNA synthetase (HisRS).

5 a hexacoordinate hemoglobin in both the bis-histidyl and the exogenously coordinated states.

6 entrations <50 microM, Pt-TP modifies mostly histidyls and abolishes half of the observed Mn inhibiti

7 The EnvZ/OmpR histidyl-aspartyl phosphorelay (HAP) system in Escherich

8 These studies provide new insights into histidyl-aspartyl phosphoryl transfers in two-component

9 as a hemichrome spectrum indicative of a bis-histidyl axial coordination and is seen clearly when the

10 ts of Chlamydomonas reinhardtii in which the histidyl axial ligand to the Mg(2+) of the P(700) chloro

11 We integrate these histidyl-catalyzed phosphorylations into a complete preb

12 Similar results were obtained when histidyl-catechol compounds linked at C-7 of the catecho

13 that mutations of two cysteinyl codons and a histidyl codon in the first 42 residues of AMT1 do not a

14 assigned to the d9 form of an iron-nitrosyl-histidyl complex of the mitochondrial protein.

15 Our findings reveal a striking bis-histidyl configuration in which both the proximal and th

16 In all cases, bis-histidyl coordination greatly increases the rate of redu

17 lass of proteins that exhibit reversible bis-histidyl coordination of the heme iron while retaining t

18 nusual class of hemoglobins that display bis-histidyl coordination yet are able to bind exogenous lig

19 ed in this study are consistent with the FAD-histidyl covalent linkage being important for the optima

20 Demodification of the carboxyethyl histidyl derivatives by hydroxylamine led to nearly comp

21 (4)(S-Cys)(5)(N-His) cluster with a bridging histidyl-derived nitrogen.

22 strated that Ca(2+) unloading from proteins, histidyl dipeptides (HDPs; e.g., carnosine), and ATP can

23 dopa) and undergo extensive stabilization by histidyl-dopa cross-link formation.

24 y proposed, but it is rather as 8 alpha-(N3- histidyl)FMN coenzyme.

25 The 8 alpha-(N3-histidyl)FMN found in corynebacterial sarcosine oxidase

26 , NAD+) and covalently bound FMN [8alpha-(N3-histidyl)FMN] which is attached to the beta subunit.

27 ulfite adduct with the covalent 8alpha-(N(3)-histidyl)-FMN.

28 that the alkaline low-spin species is a bis(histidyl) heme derivative.

29 s are attributable to the formation of a bis(histidyl) heme iron complex in both proteins at high pH.

30 acterium Synechocystis sp. PCC 6803 is a bis-histidyl hexacoordinate complex in the absence of exogen

31 stood, and little information exists for bis-histidyl hexacoordinate proteins.

32 Histidyl-histidine (His-His) has been synthesized in a y

33 c spectral shifts, the bonds to the internal histidyl imidazole ligand and those of the Fe-CO and Fe-

34 a corresponding weakening of the trans-axial histidyl imidazole linkage at lower pH.

35 proposed that singlet oxygen reacts with the histidyl imidazole ring to form an endoperoxide and then

36 s that all nine lectins are coordinated to a histidyl imidazole, with similar electron-nuclear coupli

37 talyze phosphorylations via a phosphorylated histidyl intermediate.

38 aminoacyl transfer RNA synthetases, such as histidyl (Jo-1), threonyl (PL-7), alanyl (PL-12), glycyl

39 l-Aspartyl-l-boroProline (Asp-boroPro) and l-Histidyl-l-boroProline (His-boroPro), are reported here

40 as measured by using the tripeptide hippuryl-histidyl-leucine (HHL), as model peptide, and HPLC-DAD,

41 ot bind O2 despite having a porphyrin with a histidyl ligand like the globins.

42 ydrochloride (EDC), is not associated with a histidyl ligand.

43 At least one of the two histidyl ligands (either His337 on D1 or another unident

44 octahedral coordination with four equatorial histidyl ligands and axial cysteinate and monodentate gl

45 iants; likewise, (14)N ENDOR measurements of histidyl ligands bound to Fe show no difference between

46 eolytic enzyme studies indicate that the two histidyl ligands identified by the DPC-inhibition assay

47 Mn2+ ions bind to a second carboxyl and two histidyl ligands, and these Mn are not photooxidized by

48 is corresponds to one of the axes with trans-histidyl ligands.

49 ion axis corresponding to an axis with trans-histidyl ligands.

50 ified four amino acid (two carboxyls and two histidyls) ligands to four Mn2+ bound with high affinity

51 tures that are similar to those of other bis-histidyl ligated globins, such as neuroglobin and cytogl

52 /N epsilon 2 coordination encountered in bis-histidyl ligated heme proteins.

53 as replaced with a histidine to create a bis-histidyl ligated iron typical of b-type cytochromes.

54 hemes are six-coordinate, low-spin, and bis-histidyl ligated.

55 consistent with heme peroxidases that have a histidyl-ligated heme iron.

56 nfirms both the putative globin fold and bis-histidyl ligation and also demonstrates key structural f

57 The variant structure confirms a bis-histidyl ligation but reveals unusual features.

58 estigate the contribution of the 8alpha-N(3)-histidyl linkage of FAD to the protein toward the reacti

59 tein via histidine 99 through an 8alpha-N(3)-histidyl linkage.

60 Single-flash experiments show that histidyl modification does not eliminate the binding of

61 SII membranes with a novel and more specific histidyl modifier, platinum(II) (2,2':6',2"-terpyridine)

62 structure of pyruvate phosphate dikinase, a histidyl multiphosphotransfer enzyme that synthesizes ad

63 nowledge, this is the first example of a bis-histidyl N delta 1/N epsilon 2-coordinated protoporphyri

64 relaxation analysis of the deoxy Mb proximal histidyl NdeltaH indicates that the Mb rotational correl

65 y four carboxylate oxygen atoms and a single histidyl nitrogen atom.

66 e EPR signal of D1-D170H PSII particles, the histidyl nitrogen modulation observed at 4-5 MHz is unch

67 The histidyl nitrogen modulation observed near 5 MHz in ESEE

68 but the hyperfine couplings to the ligating histidyl nitrogen of D1-His170 are too large or anisotro

69 milar alterations to the transit peptides of histidyl- or cysteinyl-tRNA synthetase, which are dual-t

70 We demonstrate that histidyl peptides catalyze phosphorylations via a phosph

71 ving an ATP analog (imidazole phosphate) and histidyl peptides, which function as organocatalytic enz

72 ng mode needed for effective transfer of the histidyl phosphate of P1 to the substrate proteins CheY

73 ry of phosphotransfer from the sensor kinase histidyl phosphate.

74 The histidyl-proline moiety of 4.1G-CTD is required for FKBP

75 termediate, which we propose to be a Cu(II)-(histidyl radical), then reacts with a nearby tyrosine re

76 The obligatory histidyl residue (His-57 in hPEPT1 and His-87 in hPEPT2)

77 HPr is either phosphorylated on a histidyl residue (HPr-P) or non-phosphorylated (HPr).

78 tire cytochrome c oxidase in having a single histidyl residue and three conserved cysteines residues

79 ported by the findings that mutation of this histidyl residue in hPEPT1 did not interfere with transp

80 In the deoxy form the proximal histidyl residue in the beta-subunit of rHb (betaV67F) h

81 The second conserved histidyl residue is located in the fourth putative trans

82 The third conserved histidyl residue is present in the cytoplasmic loop betw

83 mechanism by which the phosphorylation of a histidyl residue located 25 A from the active site resul

84 -induced polymerization, whereas a different histidyl residue on a different tubulin monomer is invol

85 The third histidyl residue was found on alpha-tubulin at alpha88.

86 volved in binding the corrinoid, including a histidyl residue which ligates cobalt.

87 These results demonstrate the involvement of histidyl residue(s) in the UDP-GlcUA uptake process in r

88 Two conserved cysteines and a histidyl residue, known to be important for both copper

89 e ionizable group, likely the imidazole of a histidyl residue.

90 nding an flavin mononucleotide cofactor to a histidyl residue.

91 pes that supported the identification of the histidyl residues 455H, 466H and 469H as chlorophyll lig

92 utant cell lines were recovered in which the histidyl residues 455H, 466H and 469H were altered.

93 The sum of the contributions from 24 surface histidyl residues accounted for 86% of the alkaline Bohr

94 Three histidyl residues are conserved among the intestinal and

95 Histidyl residues are known to be essential for the cata

96 Thus, two charged histidyl residues are obligatorily involved in vinblasti

97 he shifts of the C2 proton resonances of the histidyl residues as a function of pH.

98 Our results show that some histidyl residues contribute to the Bohr effect and some

99 bule assembly, at which time approximately 4 histidyl residues had been modified.

100 For complete inhibition two histidyl residues have to be modified.

101 We have individually mutated each of these histidyl residues in hPEPT1 and in hPEPT2 and compared t

102 xyformic anhydride, which modifies essential histidyl residues in ISP.

103 of 1,2-quinones and p-quinone methides with histidyl residues in proteins incorporated into the inse

104 at both modifiers affect the same observable histidyl residues in PSII.

105 lp us assess the contribution of the surface histidyl residues in the alpha-chain to the alkaline Boh

106 s of the C2 proton resonances of the surface histidyl residues in these Hb variants in both the deoxy

107 1 and His-87 in hPEPT2 are the most critical histidyl residues necessary for the catalytic function o

108 ride, the overall contributions from surface histidyl residues of both the alpha- and beta-chain and

109 esonances arising from the C2 protons of the histidyl residues of Hb A as a function of pH and buffer

110 The individual pK values of 24 histidyl residues of Hb A were also measured in deuteriu

111 complete the assignments for all 24 surface histidyl residues of human normal adult hemoglobin.

112 ied the C2 proton resonances of five surface histidyl residues of the alpha-chain, alpha20, alpha50,

113 fied the C2 proton resonances of two surface histidyl residues of the beta chain, beta116His and beta

114 Modification of histidyl residues of tubulin with diethylpyrocarbonate (

115 eling with [14C]DEPC localized both of these histidyl residues on beta-tubulin at beta227 and beta264

116 onances have been assigned to the individual histidyl residues on the surface of the hemoglobin molec

117 ogen-deuterium exchange and accessibility of histidyl residues to modification by diethyl pyrocarbona

118 ish or reverse the contributions of specific histidyl residues to the overall Bohr effect.

119 fect on the contributions of several surface histidyl residues which are altered because of the envir

120 dependent enzymes (IbetaH(His)) hydroxylates histidyl residues with l-threo stereospecificity.

121 heme pocket region (both proximal and distal histidyl residues), is different from that of CO- and de

122 Among those surface histidyl residues, beta146His has the biggest contributi

123 ormational changes involving several surface histidyl residues, e.g., beta146His and beta2His.

124 ssigned individual pK values for all surface histidyl residues, it is now possible to evaluate the Bo

125 Under these conditions DEPC reacts only with histidyl residues.

126 roximately 7.0 suggesting the involvement of histidyl residues.

127 cause of its greater specificity for protein histidyl residues.

128 ound flavin which is released as 8 alpha-(N3-histidyl)riboflavin upon complete hydrolysis of the prot

129 nidation reaction, we examined the effect of histidyl-specific irreversible inhibitors on the uptake

130 he substrates histidine, ATP, and 5'-O-[N-(l-histidyl)sulfamoyl]adenosine to MDCC-HisRS produced fluo

131 presence of an adenylate analogue 5'-O-[N-(L-histidyl)sulfamoyl]adenosine, HSA, decreased the apparen

132 ZntA was expressed as a histidyl-tagged protein, solubilized from membranes with

133 (either His337 on D1 or another unidentified histidyl) that bind nonphotooxidizable, high-affinity Mn

134 Anti-Jo-1 (histidyl-transfer RNA [tRNA] synthetase) and other MSAs

135 The strong association of autoantibodies to histidyl-transfer RNA synthetase (HisRS, Jo-1) with inte

136 s and in animal models support a key role of histidyl-transfer RNA synthetase (HisRS; also known as J

137 Twelve patients had anti-histidyl-transfer RNA synthetase autoantibody (anti-Jo-1

138 as a risk factor for the development of anti-histidyl tRNA synthetase antibodies, and HLA-DRB1*11:01

139 Gcn2p has a regulatory region homologous to histidyl tRNA synthetase enzymes that binds uncharged tR

140 ozygosity for mutations in the mitochondrial histidyl tRNA synthetase HARS2 at two highly conserved a

141 Thg1p-depleted cells is uncharged, although histidyl tRNA synthetase is active and the 3' end of the

142 lls having a temperature-sensitive mutant of histidyl tRNA synthetase, p70(s6k) was suppressed by a t

143 tion requires binding of uncharged tRNA to a histidyl tRNA synthetase-related domain in GCN2.

144 ing of uncharged tRNA to a domain related to histidyl tRNA synthetase.

145 equire the GCN2 regulatory domain related to histidyl tRNA synthetases.

146 The histidyl-tRNA from Escherichia coli is distinguished by

147 attenuation mechanism in which the level of histidyl-tRNA serves as a key sensor of the intracellula

148 . coli which restore histidine identity to a histidyl-tRNA suppressor carrying U73.

149 Recently, a mutation in histidyl-tRNA synthetase (HARS) was identified in a sing

150 One example from human histidyl-tRNA synthetase (HARS), termed HARS(WHEP) becau

151 ture of the closely related Escherichia coli histidyl-tRNA synthetase (HisRS) as a guide, two mutants

152 exhibits significant sequence identity with histidyl-tRNA synthetase (HisRS) but does not aminoacyla

153 ion binds to sequences in GCN2 homologous to histidyl-tRNA synthetase (HisRS) enzymes, leading to enh

154 sociating with Gcn2p sequences homologous to histidyl-tRNA synthetase (HisRS) enzymes.

155 Crystal structures of histidyl-tRNA synthetase (HisRS) from the eukaryotic par

156 hia coli, the aminoacylation of tRNA(His) by histidyl-tRNA synthetase (HisRS) is highly dependent upo

157 ement in histidine tRNAs and residues in the histidyl-tRNA synthetase (HisRS) motif 2 loop.

158 Autoantibodies to histidyl-tRNA synthetase (HisRS) or to alanyl-, asparagi

159 GCN2 contains a regulatory domain related to histidyl-tRNA synthetase (HisRS) postulated to bind mult

160 In class II histidyl-tRNA synthetase (HisRS) the nonbridging S(p)-ox

161 This is the major recognition element for histidyl-tRNA synthetase (HisRS) to permit acylation of

162 domains of the homodimeric Escherichia coli histidyl-tRNA synthetase (HisRS) were separately express

163 In class II histidyl-tRNA synthetase (HisRS), amino acid activation

164 Gcn2 activation requires a domain related to histidyl-tRNA synthetase (HisRS), the enzyme that aminoa

165 noacyl transfer in class II Escherichia coli histidyl-tRNA synthetase (HisRS), we devised a rapid que

166 CN2, requires binding of uncharged tRNA to a histidyl-tRNA synthetase (HisRS)-like domain in GCN2.

167 rved cells on binding of uncharged tRNA to a histidyl-tRNA synthetase (HisRS)-related domain.

168 domains, including a pseudokinase domain, a histidyl-tRNA synthetase (HisRS)-related region, and a C

169 ing of uncharged tRNA to a domain related to histidyl-tRNA synthetase (HisRS).

170 ministration of bacterially expressed murine histidyl-tRNA synthetase (HRS) triggers florid muscle in

171 nst nuclear and cytoplasmic Ags that include histidyl-tRNA synthetase (Jo-1).

172 has expanded; antibodies to the autoantigen histidyl-tRNA synthetase (Jo1) being the commonest and b

173 catalytic domain and a domain homologous to histidyl-tRNA synthetase and by the ability of dGCN2 to

174 ystal structure of the Staphylococcus aureus histidyl-tRNA synthetase apoprotein has been determined

175 to isolate secondary site revertants in the histidyl-tRNA synthetase from E. coli which restore hist

176 Moreover, the cytosolic histidyl-tRNA synthetase in A. castellanii exhibits an u

177 at the level of binding by Escherichia coli histidyl-tRNA synthetase is addressed by filter binding,

178 ent chemical modification experiments in the histidyl-tRNA synthetase system, emphasizes that substra

179 d sequence of tRNA(His) and at many sites in histidyl-tRNA synthetase that might be expected to affec

180 and essential for recognition by the cognate histidyl-tRNA synthetase to allow efficient His-tRNA(His

181 site fragments of Escherichia coli Class II histidyl-tRNA synthetase were constructed, expressed as

182 catalytic core of the contemporary class II histidyl-tRNA synthetase whose members lack aminoacylati

183 Specifically, M88 recognizes histidyl-tRNA synthetase, an antigen known to be also ta

184 necessary for the proper functioning of the histidyl-tRNA synthetase, and suggests a novel mechanism

185 required for aminoacylation of tRNA(His) by histidyl-tRNA synthetase, both in vitro and in vivo.

186 f mutations in HARS2, encoding mitochondrial histidyl-tRNA synthetase, mutations in CLPP expose dysfu

187 , including, in some mice, autoantibodies to histidyl-tRNA synthetase, the most common specificity fo

188 c interaction between MA and HO3, a putative histidyl-tRNA synthetase, was demonstrated in this syste

189 substitute for the P stalk in binding to the histidyl-tRNA synthetase-like domain of Gcn2 for eIF2alp

190 mino acids by binding of uncharged tRNA to a histidyl-tRNA synthetase-like domain.

191 Flanking the carboxyl terminus of the histidyl-tRNA synthetase-related domain is a region span

192 ation of mutants in the gene (hisS) encoding histidyl-tRNA synthetase.

193 f G(-1) to allows efficient histidylation by histidyl-tRNA synthetase.

194 d that GCN2 sequences containing homology to histidyl-tRNA synthetases (HisRS) bind uncharged tRNA th

195 The Gcn2p regulatory domain homologous to histidyl-tRNA synthetases is proposed to bind to uncharg

196 yeast, GCN2, contains a region homologous to histidyl-tRNA synthetases juxtaposed to the kinase catal

197 four HisZ regulatory subunits that resemble histidyl-tRNA synthetases.

198 four HisZ regulatory subunits that resemble histidyl-tRNA synthetases.

199 d CUC, have a single central-mismatch to the histidyl-tRNAQUG anticodon.

200 g events such as the selection of noncognate histidyl-tRNAQUG at the central position of the codon.

201 (Thg1) enzyme, and no examples of eukaryotic histidyl-tRNAs that lack this essential element have bee

202 ng these amino acid residues are the surface histidyls, which account for the majority of the Bohr ef