ライフサイエンスコーパス: hnRNP K

1 hnRNP K binds the TATA-binding protein, explaining how t

2 hnRNP K directly binds to C-rich single-stranded DNA wit

3 hnRNP K is a substrate of the ubiquitin E3 ligase MDM2 a

4 hnRNP K is an unusually diverse regulator of multiple st

5 hnRNP K protein was bound to antioxidant NFE2L2 transcri

6 hnRNP K regulates cellular programs, and changes in its

7 hnRNP K was also found to support expression of several

8 hnRNP K, a member of the family of heterogeneous ribonuc

9 ce-selective mRNA endonuclease, PMR-1, and a hnRNP K homology-domain RNA-binding protein, vigilin.

10 otein, either in vivo or in vitro, abrogated hnRNP K binding most likely by preventing single strand

11 non-coding RNA bearing multiple copies of an hnRNP K RNA-binding consensus sequence found within thes

12 K2 implying that adaptation of the ancestral hnRNP K and CK2 to associate with viral regulatory ances

13 The target sites for alphaCP-2KL and hnRNP K were both enriched for cytosine bases and were p

14 omplex in the absence of associated AUF1 and hnRNP K.

15 such as AU-rich binding factor 1 (AUF1) and hnRNP K have suggested that the alpha-complex is a multi

16 rom those of the closely related hnRNP E and hnRNP K proteins are undefined.

17 us nuclear ribonucleoprotein E (hnRNP E) and hnRNP K allow it to recognize longer RNA sequences.

18 The sumoylation of hnRNP A1, hnRNP F, and hnRNP K were confirmed in vivo by coimmunoprecipitation.

19 ocus that mirrored the changes in Pol II and hnRNP K profiles.

20 Both JNK inhibition and hnRNP K knockdown inhibited axon outgrowth and translati

21 immunoprecipitation, siRNA interference, and hnRNP K overexpression demonstrate that hnRNP K can regu

22 ies, we demonstrated that both nucleolin and hnRNP K bind selectively to the G- and C-rich sequences,

23 We suggest that ORF57 and hnRNP K interaction may modulate ORF57-mediated regulati

24 thermore, in response to DNA damage, p53 and hnRNP K are recruited to the promoters of p53-responsive

25 ose that the direct interaction of Sam68 and hnRNP K adversely affect the activities of both proteins

26 protein-protein interaction between TBP and hnRNP K.

27 '-untranslated region contains Pur alpha and hnRNP-K.

28 am 68, Wiskott-Aldrich Syndrome protein, and hnRNP-K, but not Cbl and Fyn, were bound to the Itk SH3

29 Antisense hnRNP K morpholino oligonucleotides (MOs) microinjected

30 ulated post-transcriptionally by hnRNP K, as hnRNP K knockdown yielded comparable defects in their nu

31 stabilization of the reporter mRNA, because hnRNP K increased the steady-state level of the reporter

32 o a thermodynamically stable complex between hnRNP K and the unfolded i-motif.

33 ided evidence for direct interaction between hnRNP K and YB-1.

34 further exploration of the interplay between hnRNP K (or other hnRNPs) and Nrf2-mediated antioxidant

35 pitation of a complex formed in vivo between hnRNP K and epitope-tagged TBP as well as binding in vit

36 rray revealed an inverse correlation between hnRNP-K expression and AR protein levels in organ-confin

37 ent protein, the RNA for which does not bind hnRNP K. hnRNP K knockdown compromised NF-M mRNA nucleoc

38 by dissecting the functional DNA/RNA-binding hnRNP K domains.

39 tions as a basal promoter element that binds hnRNP K.

40 ment protein (NF-M), the RNA for which binds hnRNP K, with that of peripherin, another intermediate f

41 K, and ERK) at the EGR-1 locus resemble both hnRNP K and RNA polymerase II (Pol II).

42 e decay rate of LDLR mRNA is not affected by hnRNP K siRNA transfection, whereas the LDLR promoter ac

43 evidence that regulation of axonogenesis by hnRNP K occurs largely through pleiotropic effects on cy

44 transcriptional activation of CT element by hnRNP K.

45 riptional regulatory pathway orchestrated by hnRNP K that is essential for successful CNS axon regene

46 within the 4CT element and is recognized by hnRNP K, which leads to a low level of transcription act

47 IAV-induced splicing events are regulated by hnRNP K, a host protein required for efficient splicing

48 For further study of regulation by hnRNP K of the cytoskeleton during axon outgrowth, we fo

49 were co-regulated post-transcriptionally by hnRNP K, as hnRNP K knockdown yielded comparable defects

50 ggest that translational inhibition of AR by hnRNP-K may occur in organ-confined tumors but possibly

51 on increases the recognition of viral RNA by hnRNP-K in the cytoplasm, promoting the function of the

52 transcription-repressive complex containing hnRNP-K/L proteins and show that knockdown of these fact

53 At 11 d after optic nerve crush, hnRNP K underwent significant translocation into the nuc

54 ors and a substantial decline in cytoplasmic hnRNP-K in metastases, despite an overall increase in hn

55 geneous nuclear ribonucleoprotein (hnRNP) D, hnRNP K and grainyhead-like 2 (GRHL2).

56 ng to known splicing factors including DDX5, hnRNP K, and PRPF6.

57 Overall, our studies demonstrate hnRNP K to be a multifunctional protein that supports VS

58 r ribonucleoprotein K (hnRNP K), designating hnRNP K as the first known pan-granzyme substrate.

59 uninjured eyes, it efficiently knocked down hnRNP K expression in only the RGCs, without inducing ei

60 y the mRNAs bound to the hnRNP-A1, hnRNP-E2, hnRNP-K, and La/SSB RBPs in BCR/ABLtransformed myeloid c

61 These findings establish hnRNP K as a new critical regulator of synaptic transmis

62 These findings establish hnRNP K as a new HDM2 target and show that, by serving a

63 alization domain exhibited less affinity for hnRNP K in vitro.

64 tiation of hnRNP K-targeted RNAs but not for hnRNP K intracellular localization or RNA binding.

65 ranscription in vitro, suggesting a role for hnRNP K in activating transcription through this single-

66 These results identify a role for hnRNP K-like genes in the structural and functional orga

67 acteristics, the optimal target sequence for hnRNP K is composed of a single short "C-patch" compatib

68 equence RNA-binding domain (CS-RBD) and four hnRNP K homology (KH) domains.

69 BP, a 577 amino acid protein containing four hnRNP K-homology domains, two RNP domains, an RGG RNA-bi

70 A functional hnRNP-K binding site involved in down-regulating AR prot

71 transcriptional regulatory modules that have hnRNP K as an essential element.

72 How hnRNP K contributes to the analgesic effects of morphine

73 haromyces cerevisiae homologues of the human hnRNP K, PBP2 and HEK2 (heterogeneous nuclear RNP K-like

74 g DNA affinity chromatography, we identified hnRNP K as a 4EBE-binding protein.

75 If hnRNP K is a transcription factor, then interactions wit

76 Together, these data implicate hnRNP K in the development of hematological disorders an

77 lysis of spatial structures of KH domains in hnRNP K and S3 reveals that they are topologically dissi

78 Finally, we find an increase in hnRNP K in nuclear speckles upon IAV infection, which ma

79 n of human tumor xenografts and reduction in hnRNP K levels in athymic mice.

80 n metastases, despite an overall increase in hnRNP-K levels in metastatic tumors.

81 We provide evidence that morphine increases hnRNP K protein expression via MOR activation in rat pri

82 r heterogeneous nuclear ribonucleoprotein K (hnRNP K or HNRPK) in a dose- and kinase-dependent manner

83 r heterogeneous nuclear ribonucleoprotein K (hnRNP K) and a 10mer ssDNA.

84 s heterogeneous nuclear ribonucleoprotein K (hnRNP K) and ribosomal protein S3.

85 f heterogeneous nuclear ribonucleoprotein K (hnRNP K) as a protein that specifically interacts with S

86 y heterogeneous nuclear ribonucleoprotein K (hnRNP K) as being rapidly induced by DNA damage in a man

87 Heterogeneous nuclear ribonucleoprotein K (hnRNP K) binds to the promoter region of mu-opioid recep

88 h heterogeneous nuclear ribonucleoprotein K (hnRNP K) in the nucleus and acts as a transcription fact

89 e heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a member of the family of hnRNPs and was rec

90 n heterogeneous nuclear ribonucleoprotein K (hnRNP K) is an RNA- and DNA-binding protein implicated i

91 Heterogeneous nuclear ribonucleoprotein K (hnRNP K) is an RNA-binding protein implicated in RNA met

92 f heterogeneous nuclear ribonucleoprotein K (hnRNP K) protein by human protein arginine methyltransfe

93 r heterogeneous nuclear ribonucleoprotein K (hnRNP K) was found to bind selectively to the i-motif sp

94 f heterogeneous nuclear ribonucleoprotein K (hnRNP K), a multifunctional cellular protein involved in

95 n heterogeneous nuclear ribonucleoprotein K (hnRNP K), a protein with a conserved KH motif and RGG bo

96 n heterogeneous nuclear ribonucleoprotein K (hnRNP K), designating hnRNP K as the first known pan-gra

97 n heterogeneous nuclear ribonucleoprotein K (hnRNP K), which binds the GLT1/EAAT2 promoter.

98 g heterogeneous nuclear ribonucleoprotein K (hnRNP K), which binds the single stranded sequence (CCCT

99 s heterogeneous nuclear ribonucleoprotein K (hnRNP K), which has been demonstrated to be a transcript

100 d heterogeneous nuclear ribonucleoprotein K (hnRNP K).

101 n heterogeneous nuclear ribonucleoprotein K (hnRNP K).

102 t heterogeneous nuclear ribonucleoprotein K (hnRNP-K) and Pur(alpha) act together to mediate repressi

103 , heterogeneous nuclear ribonucleoprotein K (hnRNP-K), by mass spectrometric analysis of Akt immune c

104 in, the RNA for which does not bind hnRNP K. hnRNP K knockdown compromised NF-M mRNA nucleocytoplasmi

105 These findings functionally integrate K17, hnRNP K, and gene expression along with RSK and CXCR3 si

106 The K17-hnRNP K partnership is regulated by the ser/thr kinase R

107 KH (hnRNP K homology) domains, consisting of approximately 7

108 cific recognition of loop B by the first KH (hnRNP K homology) domain of cellular poly(rC)-binding pr

109 The third and fourth KH (hnRNP K homology) domains of ZBP1 specifically recognize

110 The third and fourth KH (hnRNP K homology) domains of ZBP1 specifically recognize

111 PCBPs contain three copies of KH (hnRNP K homology) domains, which are responsible for bin

112 cells is achieved by a subfamily of the KH (hnRNP K homology) domain-containing proteins known as po

113 t regulatory proteins that contain three KH (hnRNP K homology) domains.

114 n, but not mRNA, expression of several known hnRNP K RNA targets (NF-M, GAP-43) by compromising their

115 rformed two-hybrid screens using full-length hnRNP K as a bait.

116 At the molecular level, hnRNP K knockdown during regeneration inhibited protein,

117 nt that these viruses exploit the CXCL8/MAPK/hnRNP-K axis to enhance viral replication in respiratory

118 For maximal hnRNP K transcription activation, two additional cytosin

119 This growth factor-mediated hnRNP K expression was effectively blocked by pretreatme

120 grade III human breast cancer contained more hnRNP K protein than samples from grade II cancer.

121 Moreover, hnRNP K increased translation initiation, increased cell

122 Moreover, hnRNP K knockdown prevents ERK cascade activation and Gl

123 d, facilitating the translocation of nuclear hnRNP-K to the cytoplasm.

124 itor its DNA binding in vivo, the ability of hnRNP K to activate a reporter gene was amplified by fus

125 Abrogation of hnRNP K sumoylation leads to an aberrant regulation of t

126 ere not adversely affected in the absence of hnRNP K, whereas viral genome transcription and replicat

127 infection, which may alter accessibility of hnRNP K for host transcripts thereby leading to a progra

128 utes to the morphine-induced accumulation of hnRNP K protein in regions of the central nervous system

129 ed whether morphine-mediated accumulation of hnRNP K resulted from translational control.

130 sphorylation and cytoplasmic accumulation of hnRNP K.

131 nt did not change the strength of binding of hnRNP K to Sam68.

132 Cleavage of hnRNP K was more efficient in the presence of RNA and oc

133 dentify the role of translational control of hnRNP K in morphine-induced analgesia through activation

134 We show that depletion of hnRNP K by siRNA transfection reduces the expression of

135 Additionally, depletion of hnRNP K resulted in not only significantly increased lev

136 of the C-terminal K-homology (KH) domain of hnRNP K by NMR spectroscopy.

137 h contain the three proline-rich domains, of hnRNP K.

138 e protein relieved the suppression effect of hnRNP K on the activity of the human thymidine kinase ge

139 vious study indicated that the expression of hnRNP K activates c-myc promoter in transient transfecti

140 GF and heregulin-beta1 induced expression of hnRNP K mRNA and protein in human breast cancer cells.

141 We show that the expression of hnRNP K results in a trans-activation of a variety of RN

142 Over-expression of hnRNP K, like NO*, repressed translation of CURE-contain

143 One or more alphaCPs, members of a family of hnRNP K-homology domain poly(C) binding proteins, are es

144 omimetic, but not phosphodeficient, forms of hnRNP K.

145 hnRNP K suggests that multiple functions of hnRNP K may be disrupted by the core protein during HCV

146 cal, which encodes the Drosophila homolog of hnRNP K.

147 Induction of hnRNP K ensues through the inhibition of its ubiquitin-d

148 ecessary for the translational initiation of hnRNP K-targeted RNAs but not for hnRNP K intracellular

149 tation assays reveal a direct interaction of hnRNP K with the LDLR promoter in intact HepG2 cells.

150 species and destabilizes the interaction of hnRNP K with the Mid-region i-motif.

151 This is the first structure investigation of hnRNP K.

152 For insights into the involvement of hnRNP K in neuronal post-transcriptional gene control at

153 siRNA knockdown of hnRNP K decreased the levels of active MEK and ERK at th

154 cts were specifically caused by knockdown of hnRNP K expression.

155 for tumor cell viability, since knockdown of hnRNP K resulted in spontaneous tumor cell apoptosis wit

156 required for the cytoplasmic localization of hnRNP K and for its role in regulating the expression of

157 The C-terminal KH module of hnRNP K (KH3) is revealed to be a three-stranded beta-sh

158 Finally, overexpression of hnRNP K in breast cancer cells significantly increased t

159 Furthermore, overexpression of hnRNP K increased the LDLR promoter activity by the luci

160 alyses indicated that JNK phosphorylation of hnRNP K occurred within the cytoplasm and was necessary

161 e Pol II transcription-driven recruitment of hnRNP K along the EGR-1 locus compartmentalizes activati

162 unoprecipitations to study co-recruitment of hnRNP K and ERK cascade activity along the EGR-1 gene.

163 ts located in the 5' untranslated regions of hnRNP K transcripts that were regulated by morphine.

164 Finally, we found that down-regulation of hnRNP K mediated by siRNA attenuated morphine-induced hy

165 Here, we investigated the role of hnRNP K in synapse function.

166 To decipher the role of hnRNP K in VSV infection, we conducted studies which sug

167 ivation function depends on the sequences of hnRNP K that are also necessary for RNA binding.

168 Silencing of hnRNP K protein expression rendered tumor cells more sus

169 ility and our data suggest that targeting of hnRNP K by granzymes contributes to or reinforces the ce

170 inhibited axon outgrowth and translation of hnRNP K-regulated cytoskeletal RNAs (tau and neurofilame

171 Furthermore, knockdown of hnRNP-K inhibits MBP protein synthesis during myelinatio

172 required for proper genomic localization of hnRNP-K at repressed genes and regulation of p53 mediate

173 ay, suppression of Nmi and overexpression of hnRNP-K and c-myc proteins may explain why the prostate

174 in destabilization through the regulation of hnRNP-K controlled ubiquitin -ligase translation identif

175 cing by preventing the redundant activity of hnRNPs K and E2 to compensate for the weakened function

176 A)-mediated silencing of either nucleolin or hnRNP K resulted in the down-regulation of basal VEGF ge

177 CK2 modification enhanced the ORF57-hnRNP K interaction, and may regulate the presence and a

178 ates, but does not abolish, their outgrowth, hnRNP K must target additional RNAs needed for axon deve

179 show that, by serving as a cofactor for p53, hnRNP K plays key roles in coordinating transcriptional

180 ogeneous nuclear ribonucleoprotein particle (hnRNP) K protein is comprised of multiple modular domain

181 within these transcripts largely phenocopied hnRNP K knockdown, further supporting the idea that it r

182 nt with the presence of additional predicted hnRNP-K binding sites within the AR open reading frame a

183 t K17 interacts with the RNA-binding protein hnRNP K, which has also been implicated in cancer.

184 The RNA-binding protein, hnRNP K, is essential for axonogenesis.

185 t c-myc promoter activity and c-Myc protein, hnRNP K protein levels, and enhanced breast cancer cell

186 complex, consisting of RNA-binding proteins (hnRNP K, FXR1, and FXR2), PUF60 and SF3B3, that is requi

187 Direct addition of recombinant hnRNP K to reaction mixtures programmed with templates b

188 Affinity columns charged with recombinant hnRNP K specifically bind a component(s) necessary for t

189 Reduced hnRNP K expression attenuated p21 activation, downregula

190 human EGF receptor family members regulates hnRNP K expression by extracellular growth promoting sig

191 nctional transcription/translation regulator hnRNP-K and the mRNA-encoding myosin regulatory light-ch

192 duced negative superhelicity, where relative hnRNP K and nucleolin expression shifts the equilibrium

193 ly characterized as a protein that resembles hnRNP K and which binds to a single-stranded, pyrimidine

194 These results not only reveal hnRNP K to be a single strand DNA binding protein in viv

195 the heterogeneous nuclear ribonucleoprotein hnRNP K.

196 ied heterogeneous nuclear ribonucleoprotein (hnRNP) K and hnRNP E1 as the proteins forming the 70- an

197 ors heterogeneous nuclear ribonucleoprotein (hnRNP) K and nucleolin, respectively, both in vitro and

198 ein heterogeneous nuclear ribonucleoprotein (hnRNP) K interacts with kinases and is found along genes

199 Heterogeneous nuclear ribonucleoprotein (hnRNP) K is a nucleocytoplasmic shuttling protein that i

200 hat heterogeneous nuclear ribonucleoprotein (hnRNP) K is a transactivator of Th transcription.

201 ns, heterogeneous nuclear ribonucleoprotein (hnRNP) K is a transcription factor for the c- myc gene,

202 The heterogeneous nuclear ribonucleoprotein (hnRNP) K protein recruits a diversity of molecular partn

203 lar heterogeneous nuclear ribonucleoprotein (hnRNP) K protein with many of its protein and nucleic ac

204 The heterogeneous nuclear ribonucleoprotein (hnRNP) K, a component of the hnRNP particles, appears to

205 and heterogeneous nuclear ribonucleoprotein (hnRNP) K, a less well-characterized protein associated w

206 in, heterogeneous nuclear ribonucleoprotein (hnRNP) K, because it is required for axonogenesis during

207 and heterogeneous nuclear ribonucleoprotein (hnRNP) K.

208 entified three K-homology (KH) domain RNPs - hnRNP K, hnRNP E1, hnRNP E2 - as being capable of bindin

209 scue experiments and the use of two separate hnRNP K MOs were carried out to confirm that these effec

210 Here we report that the shuttling hnRNP K protein contains a novel shuttling domain (terme

211 Silencing hnRNP K expression in the spinal cord increased nocicept

212 Most interestingly, DNA damage stimulates hnRNP K sumoylation through Pc2 E3 activity, and this mo

213 Strikingly, hnRNP K depletion abrogates transcriptional induction of

214 pment of hematological disorders and suggest hnRNP K acts as a tumor suppressor.

215 s) microinjected into blastomeres suppressed hnRNP K expression from neural plate stages through to a

216 se Vivo-Morpholino oligonucleotide targeting hnRNP K.

217 gnition motifs (RRMs) and four COOH-terminal hnRNP K homology (KH) domains.

218 We conclude that hnRNP K is indispensable for tumor cell viability and ou

219 and hnRNP K overexpression demonstrate that hnRNP K can regulate eIF4E mRNA.

220 We demonstrated that hnRNP K regulates dendritic spine density and long-term

221 We demonstrated that hnRNP K significantly inhibited Sam68-mediated, but not

222 s provide strong evidence demonstrating that hnRNP K is an important transactivator for human LDLR ge

223 These experiments provide evidence that hnRNP K is the nexus of a novel post-transcriptional reg

224 neurofilaments, raising the hypothesis that hnRNP K post-transcriptionally regulates multiple transc

225 These data indicate that hnRNP K binds to a specific cis element, interacts with

226 These results indicate that hnRNP K is likely involved in virus assembly and/or rele

227 D phosphocellulose fraction, indicating that hnRNP K might interact with the TATA-binding protein (TB

228 We report that hnRNP K, an RNA-binding protein implicated in multiple a

229 eoprotein immunoprecipitations revealed that hnRNP K was associated with the EGR-1 but not c-MYC mRNA

230 xpressing TDP-43Q331K mutation, we show that hnRNP K expression is impaired in urea soluble extracts

231 In the present work, we show that hnRNP K is modified by SUMO in lysine 422 within its KH3

232 We show that hnRNP K KH3 specifically recognizes a tetrad of sequence

233 Further studies showed that hnRNP K suppresses apoptosis of virus-infected cells, re

234 CT-element driven reporters, suggesting that hnRNP K recognizes a single strand region generated by n

235 This suggests that hnRNP K promotes tumor cell survival in the absence of c

236 Here we report, for the first time, that hnRNP K is specifically involved in human LDL receptor (

237 We show here that hnRNP-K is a novel inhibitor of AR mRNA translation that

238 Further analysis revealed that hnRNP-K is also able to inhibit AR translation in the ab

239 The hnRNP K dependence and pattern of ERK cascade activation

240 The hnRNP K-binding site in HCV core protein was mapped to t

241 rily in the C-terminal end of hFMRP, but the hnRNP K homology domain influenced binding as well.

242 ble-stranded RNA binding domain (dsRBD), the hnRNP K homology (KH) domain and the RNP motif.

243 ave analyzed the biochemical function of the hnRNP K protein by using a mouse cDNA clone.

244 s cerevisiae that contains 14 repeats of the hnRNP K-homology (KH) domain, and demonstrates significa

245 run-on assays, we provide evidence that the hnRNP K protein trans-activates the reporter genes by in

246 The RNA-binding protein QKI belongs to the hnRNP K-homology domain protein family, a well-known reg

247 The hnRNP-K molecule and Pur(alpha) bind single-stranded DNA

248 he p53 transcriptional co-activation through hnRNP K sumoylation.

249 tion, JNK acts posttranscriptionally through hnRNP K to regulate translation of proteins crucial for

250 -rich strand of the CT element both bound to hnRNP K and competitively inhibited transcription in vit

251 effects of mutant TDP-43-mediated changes to hnRNP K metabolism by RNA binding immunoprecipitation an

252 known about the role of SUMO conjugation to hnRNP K in p53 transcriptional co-activation.

253 The specific binding of HCV core protein to hnRNP K suggests that multiple functions of hnRNP K may

254 ZBP1 and ZBP2, two hnRNP K homology domain-containing proteins, were previo

255 iR-7, which is processed from the ubiquitous hnRNP K pre-mRNA transcript, is achieved by inhibition o

256 e pronounced at low tumor cell density where hnRNP K knockdown also triggered a caspase-independent a

257 neural development demonstrated that whereas hnRNP K binding occurred at all stages, hnRNP E binding

258 -immunoprecipitated from juvenile brain with hnRNP K were identified on microarrays.

259 xtracts, ORF57 was present in a complex with hnRNP K that had protein kinase CK2 activity, and was ph

260 7 (Simplexvirus) proteins both interact with hnRNP K and CK2 implying that adaptation of the ancestra

261 The interaction of HCV core protein with hnRNP K was confirmed by glutathione S-transferase fusio

262 egrowth of axons beyond the lesion site with hnRNP K knockdown.

263 diated through the physical association with hnRNP-K.

264 e show that lincRNA-p21 acts in concert with hnRNP-K as a coactivator for p53-dependent p21 transcrip

265 lination alpha6beta1-integrin interacts with hnRNP-K, an mRNA-binding protein, which binds to MBP mRN