ライフサイエンスコーパス: hybridisation

1 tem for investigating the potential for wide hybridisation.

2 ophageal mucosa was determined using in-situ hybridisation.

3 roup were validated with comparative genomic hybridisation.

4 ce by quantitative real-time PCR and in situ hybridisation.

5 enes and matrix RNA with fluorescent in-situ hybridisation.

6 ctures, and in assessing the consequences of hybridisation.

7 Pleistocene era, and occurred introgressive hybridisation.

8 ar chemokine mRNA expression by differential hybridisation.

9 d less starting material being used for each hybridisation.

10 d plants and was chosen for study by in situ hybridisation.

11 and localisation investigated using in situ hybridisation.

12 ecies remain distinct in the face of natural hybridisation.

13 age chick embryos, using whole-mount in situ hybridisation.

14 a 3H-cDNA probe against intron 1 for in situ hybridisation.

15 d groups in the spacer diminish the yield of hybridisation.

16 nd quantitated after heat shock by slot blot hybridisation.

17 ving up to 150-fold increase in the yield of hybridisation.

18 mouse chromosome 2p by fluorescence in situ hybridisation.

19 tested peptides in the IGL/VLG using in situ hybridisation.

20 s of the nucleic acid targets as a result of hybridisation.

21 pathways that was also confirmed by in situ hybridisation.

22 nts' HPV status with p16 staining or in-situ hybridisation.

23 conditions demonstrated via the solid-phase hybridisation, a 144-bp double stranded DNA (dsDNA) was

24 Array comparative genomic hybridisation (aCGH) profiling is currently the gold sta

25 cally referred for array comparative genomic hybridisation (aCGH); cases were compared with 11,277 co

26 ding close relative can result from repeated hybridisation along the invasion front and/or allele sur

27 Such wide hybridisations also challenge accepted taxonomic classif

28 Hybridisation among taxa with different ploidy levels is

29 r understanding on reproductive barriers and hybridisation among this vector's sibling incipient spec

30 polymerase chain reaction (RT-PCR) and blot hybridisation analyses reveal that exon 6.1 is utilised

31 ata, in conjunction with fluorescent in situ hybridisation analyses, suggest that Sulfolobus chromoso

32 In situ hybridisation analysis demonstrates a normal distributio

33 Tissue print hybridisation analysis of the hypocotyl revealed that th

34 DNA hybridisation analysis showed that elements related to T

35 Fluorescence in-situ hybridisation analysis was done with probes specific for

36 ample of the interplay between ploidy level, hybridisation and alien plant invasion.

37 oach presents an alternative to mRNA in situ hybridisation and allows detection of expression in an a

38 Novel targets were validated by in situ hybridisation and binding to AG in vitro and in vivo.

39 ell expression in two novel genes by in situ hybridisation and catalogue dorsal gland promoter elemen

40 cloned by screening suppression subtractive hybridisation and cDNA libraries of cotton genotypes tol

41 We used PCR followed by Southern blot hybridisation and DNA sequence analysis to detect DNAs o

42 nsin genes in Brassica napus, using northern hybridisation and dot blotting.

43 tumour genomes by array comparative genomic hybridisation and gene expression microarray analysis.

44 pocampal gyrus) and cerebellum using in-situ hybridisation and immunoautoradiography.

45 ession of HB-EGF mRNA and protein by in-situ hybridisation and immunohistochemical techniques, respec

46 genomics, mouse transgenic analysis, in situ hybridisation and immunohistochemistry to identify a hig

47 ribution of KIS in human brain using in situ hybridisation and immunohistochemistry, and quantified K

48 mbryonic intestine was determined by in-situ hybridisation and immunohistochemistry.

49 ocated in the plasma membrane, while in situ hybridisation and immunolocalisation demonstrate the pre

50 els demonstrates that for a weak inter-chain hybridisation and intra-channel electron-electron attrac

51 ue offers potential for the investigation of hybridisation and introgression among tilapia species in

52 Hybridisation and introgression are common within tilapi

53 Hybridisation and introgression can dramatically alter t

54 In situ hybridisation and Northern blot analysis have shown that

55 idence supporting that Ug99 arose by somatic hybridisation and nuclear exchange between dikaryons.

56 in tumour is controversial, and both in-situ hybridisation and PCR are commonly used; P16 immunohisto

57 was detected in the human genome by Southern hybridisation and polymerase chain reaction.

58 By hybridisation and sequencing we found that most lambda c

59 By hybridisation and sequencing we found that the patterns

60 occurred more than once in association with hybridisation and shifts in ploidy.

61 Analysis with 24-colour fluorescence in situ hybridisation and single nucleotide polymorphism (SNP) a

62 (IDMI), enhancement of the damping, via d-d hybridisation and spin-pumping across the interface, and

63 been conserved following genome duplication, hybridisation and transitions between dioecy and hermaph

64 alisation, gene expression analysis, in situ hybridisation and virus-induced gene silencing, indicate

65 n 150 experiments corresponding to over 3000 hybridisations and supports the Microarray Centre's larg

66 RT-PCR analysis, in situ hybridisation, and cell surface-labelling of neural cres

67 two different monoclonal antibodies, in-situ hybridisation, and PCR with DNA sequencing.

68 d immunohistochemistry, fluorescence in-situ hybridisation, and tissue analysis to look for multiline

69 xamined by PCR, plasmid extraction, Southern hybridisation, and transconjugation.

70 ve long been problematic because of frequent hybridisation, apomixis and presumed rapid radiation, an

71 Hybridisation appears to substantially modify the biosyn

72 a one-step cell lysis, target labelling and hybridisation approach as well as a corresponding passiv

73 until levels below the limit of non-specific hybridisation are reached 11 h after wounding.

74 rformed microarray-based comparative genomic hybridisation (array-CGH) with male and female Duroc gen

75 ith unexplained IS using comparative genomic hybridisation arrays (aCGH) (n = 44) followed by targete

76 Using plasmid Southern blot hybridisation as a secondary screen, we are able to iden

77 PSA modified electrode and then applied in a hybridisation assay to determine the concentration of th

78 the results obtained by a commercial reverse hybridisation assay, GenoType CM/AS (Hain Lifescience, T

79 Direct detection of PCR product via hybridisation assay, would facilitate the development of

80 onformation capture and fluorescence in situ hybridisation assays were used to investigate the change

81 than along it, as predicted under recurrent hybridisation at the invasion front.

82 is typically depleted prior to sequencing by hybridisation-based methods; an alternative approach is

83 d from Haemophilus influenzae type b using a hybridisation-based strategy.

84 ce steric interference of the support on the hybridisation behaviour of immobilised oligonucleotides.

85 ry precursor of C(4) and/or as the result of hybridisation between a C(3) and C(4) lineage.

86 olyploid speciation, which generally involve hybridisation between a native and an alien species.

87 e evolutionary consequences of interspecific hybridisation between asexual females and sexual males.

88 e a widespread triploid taxon resulting from hybridisation between diploid Mimulus guttatus and tetra

89 which arose over comparable time frames from hybridisation between increasingly divergent diploid spe

90 -statistics revealed significant evidence of hybridisation between lineages co-occurring on the same

91 Hybridisation between S haematobium and the cattle schis

92 se conditions for solid-phase amplification, hybridisation between short 25-mer single stranded DNA (

93 ed in sympatry or parapatry, with occasional hybridisation between species.

94 ng light-matter coupling regime rests on the hybridisation between states with different numbers of e

95 rtebrate parthenogenetic lineages arise from hybridisation between two divergent taxa within a specif

96 Although this is consistent with "hybridisation" between the diverging human and chimp lin

97 Iberia, would be best explained by recurrent hybridisation but this is difficult to prove because the

98 lobsters threaten native populations through hybridisation, but morphological discriminants used for

99 Steric hindrance in hybridisation can also be a problem if the oligonucleoti

100 When hybridisation carries a cost, natural selection is predi

101 chromosomes--comparative expressed sequence hybridisation (CESH)--to establish if any expression pat

102 gh resolution genome-wide comparative genome hybridisation (CGH) arrays were used to compare tumour a

103 nt quantities of DNA for comparative genomic hybridisation (CGH) as well as more than 90 independent

104 amplification (MLPA) or comparative genomic hybridisation (CGH) cause 4q- syndrome.

105 Microarray based comparative genomic hybridisation (CGH) experiments have been used to study

106 polymorphism (SNP) array comparative genomic hybridisation (CGH) showed mutually exclusive endoredupl

107 and we demonstrate their use for subtractive hybridisation cloning of differentially expressed cDNAs.

108 In situ hybridisation confirmed the localisation of Mel1a mRNA t

109 We confirmed that hybridisation could be further enhanced by modifying the

110 as where Bulinus snails are endemic, and how hybridisation could increase the colonisation potential

111 s linear regression models of non-normalised hybridisation data to define methylation status.

112 lisation of markers and fluorescence in situ hybridisation data.

113 l3 and Dll1 expression by double RNA in situ hybridisation demonstrates that these genes have distinc

114 Northern hybridisation did not detect cathepsin D mRNA in either

115 Differential Methylation Hybridisation (DMH) is one technique used for genome-wid

116 Hybridisation, DNA sequencing, targeted mutagenesis, and

117 bryos using whole amount and section in-situ hybridisation do not readily allow appreciation of 3-dim

118 re also frequently involved in interspecific hybridisation events as well as being produced by inters

119 that parthenogens result from multiple past hybridisation events between species from specific linea

120 The hybridisation events were monitored by electrochemical i

121 Additional microarray hybridisation experiments allowed the identification of

122 Genetic and in situ hybridisation experiments have determined that this gene

123 However, our Northern blot hybridisation experiments indicate that the main RNA spe

124 Coupled with in situ hybridisation experiments, these analyses showed that th

125 essing tissues, both by fluorescence in situ hybridisation (FISH) and by chromosome conformation capt

126 hat EGFR copy number by fluorescence in-situ hybridisation (FISH) can identify patients most likely t

127 say can be combined with fluorescent in situ hybridisation (FISH) methodology in order to investigate

128 parate validation using fluorescence in situ hybridisation (FISH) shows that the proportions of bifid

129 x;18)(p11;q11) with the fluorescence in situ hybridisation (FISH) technique.

130 A fluorescence in-situ hybridisation (FISH) test was used to examine the integr

131 loid S. tuberosum using fluorescence in situ hybridisation (FISH) to differentiate individual meiotic

132 (standard karyotyping), fluorescent in situ hybridisation (FISH), multiplex ligation-dependent probe

133 obed with the library by fluorescent in situ hybridisation (FISH), the predominant sites of labelling

134 using a synchrotron or fluorescence in situ hybridisation (FISH).

135 13.3 [corrected] using fluorescence in situ hybridisation (FISH).

136 s of bone metastases by fluorescence in situ hybridisation (FISH).

137 over 24h incubations by fluorescence in situ hybridisation (FISH).

138 ohistochemical [IHC] or fluorescence in-situ hybridisation [FISH], or both).

139 ergence was obliterated by means of advanced hybridisation, followed by a multi-generation exposure o

140 ubclonal composition by fluorescence in-situ hybridisation for 17p(TP53) or 11q(ATM) deletion.

141 tological tests such as fluorescence in-situ hybridisation for aneusomy are helpful in the diagnosis.

142 expression and in parallel performed in situ hybridisation for RFX6 in the dorsal pancreatic bud of a

143 lysis was based on a suppression subtractive hybridisation forward library of B157 (tea clone infeste

144 ass (Lolium multiflorum) and genomic in situ hybridisation (GISH) was used to identify the introgress

145 We identified 72 hybrid systems for which hybridisation has been putatively associated with invasi

146 Geographic isolation, habitat shifts and hybridisation have all contributed to the diversificatio

147 geographical isolation, habitat shifts, and hybridisation have contributed to the evolution of diver

148 raphy of D(1) and D(2) receptors and in situ hybridisation histochemistry of D(1) and D(2) mRNA were

149 In situ hybridisation histochemistry revealed no difference in p

150 itative receptor autoradiography and in situ hybridisation histochemistry were used to study both the

151 n the rat forebrain using RT-PCR and in situ hybridisation histochemistry.

152 ur species which have often been involved in hybridisation in aquaculture: 13 for Oreochromis nilotic

153 ing the nature of fertilisation, the role of hybridisation in evolution, and aspects often considered

154 s were identified by XY fluorescence in-situ hybridisation in marrow-derived mesenchymal stem cells a

155 ll markers for all these clusters by in situ hybridisation in schistosomula and adult parasites.

156 The role of interspecific hybridisation in the evolution of pest species is poorly

157 an weaken the (Ir Jeff = 1/2)-(O 2p) orbital hybridisation in the in-planar Rh-O-Ir bond networks.

158 Here we address the role of hybridisation in the origin and evolutionary lifespan of

159 ected concurrently with fluorescence in-situ hybridisation in the same cells of a tissue section.

160 ool to investigate the prevalence of cryptic hybridisation in the wild.

161 Furthermore, using a combination of in situ hybridisation, in vivo ChIP assay and transgenic explant

162 Recent advances in fluorescent in situ hybridisation included the generation of allele-specific

163 of host-based divergence evolved faster and hybridisation-induced linkage disequilibrium decayed slo

164 sequence mismatches can have upon microarray hybridisation intensities even for long oligonucleotide

165 adiation of elapoid snakes suggest that both hybridisation/introgression during the rapid diversifica

166 review of studies experimentally testing the hybridisation-invasion (H-I) hypothesis in plants, anima

167 le to control and predict the process of DNA hybridisation is crucial for the ambitious future of Hyb

168 d specificity of multiplex amplifiable probe hybridisation is demonstrated by the simultaneous assess

169 nd possible acquisition of germplasm through hybridisation is fundamental to understanding the evolut

170 Hybridisation is increasingly recognised as an important

171 Therefore, using Ventana in situ hybridisation (ISH), quantitative PCR assays and biomark

172 In situ hybridisation localised arenavirus mostly to blood cells

173 In situ hybridisation localised Pax-7 to mononuclear cells in th

174 e was little support for the hypothesis that hybridisation may be responsible for the occurrence of n

175 Impacts of introgressive hybridisation may range from genomic erosion and species

176 Recent studies have shown that hybridisation may relax transcriptional regulation and e

177 scent field and introduces three generalised hybridisation modes that have emerged.

178 en printed electrodes the paper demonstrates hybridisation monitoring of mecA in an "on-line" assay f

179 With the hybridisation of a complementary target sequence (BPV ta

180 n of mutated mtDNA is inhibited by selective hybridisation of a nucleic acid derivative to the single

181 avidin-coated glass slides and visualised by hybridisation of fluorescent detector oligonucleotides t

182 Southern blot hybridisation of Fugu genomic DNA confirmed the SART1 ge

183 Genomic in situ hybridisation of IL pau16061, resistant and susceptible

184 The sites were detected by in situ hybridisation of labelled rDNA to chromosomal preparatio

185 ormalisation and is less influenced by cross-hybridisation of loci.

186 ctrode in PNA form it was possible to detect hybridisation of mecA PCR product electrochemically at c

187 The sp2 hybridisation of N(10) is small compared to other flavop

188 Hybridisation of NBR2 probes to zoo blots showed that th

189 The hybridisation of phonon polaritons with longitudinal pho

190 In situ hybridisation of seven candidate biomineralisation genes

191 trate that this transition occurs due to the hybridisation of states associated with different TI fil

192 azole orange) provides an anchor, which upon hybridisation of the probe to its target becomes fluores

193 Biallelic assay by hybridisation of the RCA products with fluorescence dye-

194 tainable using electrochemical detection via hybridisation of the tailed, ferrocene labelled PCR prod

195 ication was assessed by fluorescence in-situ hybridisation of two cores of breast tumour tissue in a

196 Combined with no negative effects of hybridisation on survival or reproductive characters in

197 Whole-mount in situ hybridisation on tailbud stage embryo reveals strong exp

198 We performed digital hybridisation on the NanoString platform to assess the r

199 the syndrome with FISH (fluorescence in-situ hybridisation) or PCR as rapid diagnostic tests.

200 ot analysis with cucumber DNA shows a simple hybridisation pattern indicating one or very few genes.

201 ired synchrony, as manifested in the in situ hybridisation patterns of the single mutants.

202 ed biliary tract cancer confirmed by in-situ hybridisation per central testing, at least one measurab

203 roducts that can be used directly as in situ hybridisation probes.

204 The hypothesis that interspecific hybridisation promotes invasiveness has received much re

205 candidates for the semiochemical barrier to hybridisation, providing an opportunity to characterise

206 lection-based processes involved in the post-hybridisation purge of archaic introgressed regions.

207 opheles gambiae in Guinea-Bissau, where high hybridisation rates appear to be stable at least since t

208 A net inhibition of the hybridisation reaction was observed after incubation wit

209 detection of naphtol was used to detect the hybridisation reaction.

210 e used locked nucleic acid probes in in situ hybridisation reactions to study the distribution of mic

211 dings illustrate how the combined effects of hybridisation, recombination, and natural selection, act

212 ptical spectroscopy, these results suggest a hybridisation-related mechanism, in which Rh doping can

213 Subsequent subtractive hybridisation removes sequences common to both, leaving

214 leptin in vitro autoradiography and in situ hybridisation, respectively.

215 ing previous immunohistochemical and in-situ hybridisation results.

216 gel electrophoresis and array based genomic hybridisation revealed a large-scale genomic deletion co

217 In situ mRNA hybridisation revealed accumulation of mRNA encoding eac

218 Dual in situ hybridisation revealed GPCR101 and oxytocin mRNA co-expr

219 In situ hybridisation revealed that HS 6-O-sulfotransferase is r

220 In situ hybridisation revealed that polyI:C offspring had: (1) S

221 In the supraoptic nucleus, in situ hybridisation revealed that the temporal regulation of o

222 In situ hybridisation reveals overlapping expression of MAST1 an

223 We used immunohistochemistry, in-situ hybridisation, reverse transcriptase-PCR (RT-PCR), and f

224 se confirmed by central fluorescence in-situ hybridisation review suggested marginal benefit with lap

225 We have used this system in a subtractive hybridisation screen that resulted in the cloning of Xen

226 UDMAP includes data from large-scale in situ hybridisation screens (wholemount and section) and micro

227 Herein, we report a wall- and hybridisation-selective synthetic methodology to produce

228 A DNA hybridisation sensor was also assembled using a thiolate

229 Transcriptional profiling and in situ hybridisation show that JNK signalling is upregulated in

230 Fluorescent in situ hybridisation showed that in ahp2-1 male meiocytes, chro

231 Whole-mount in situ hybridisation showed that Lhx6 and Lhx7 were expressed d

232 sessed by its presence in polysomes; in situ hybridisation showed that the mRNA was localised around

233 In situ hybridisation showed that TrkB was expressed primarily i

234 e identified that, when analysed by northern hybridisation, showed different patterns of expression d

235 Analysis of BMP4 expression by in situ hybridisation shows that this growth factor is produced

236 Additional fluorescence in situ hybridisation signals indicate the presence of several h

237 sought by sequence-specific oligonucleotide hybridisation, site-directed sequencing in Caucasian and

238 describe how array-based comparative genomic hybridisation, SNP arrays, array painting and next-gener

239 The hybridisation specificity experiments further indicated

240 nt, the number and location of p53 and hTERT hybridisation spots was recorded in addition to standard

241 nd +P cultures using suppression subtractive hybridisation (SSH) followed by 454 pyrosequencing, and

242 f both moieties is dependant on target-probe hybridisation straightening the loop.

243 In situ hybridisation studies demonstrated high levels of Nav1.2

244 Early models based upon RNA hybridisation studies suggest bursting dynamics arise fr

245 d a single message of 1.5 kb, while Southern hybridisation suggests a small multigene family of relat

246 In the presence of target miRNA-21, hybridisation takes place resulting in the "activation"

247 Using in situ hybridisation TASK-1 mRNA was found to be expressed in t

248 A subtractive hybridisation technique was developed to clone cDNAs rep

249 Immunohistochemistry and in situ hybridisation techniques that facilitate reliable and re

250 eotide array/DNA chip technology when higher hybridisation temperatures are required, for example, to

251 Cytogenetic and fluorescence in-situ hybridisation testing was performed on pre-treatment bon

252 ally a mecA specific probe was selected from hybridisation tests with a 3' and 5' version of a previo

253 y such genes and show by whole-mount in situ hybridisation that their expression pattern is that expe

254 per we demonstrate, using RT-PCR and in situ hybridisation, that Pax3 expression can serve as a marke

255 By in situ hybridisation, the histological distribution of high lev

256 minating seedling was carried out by in situ hybridisation; the strongest signals were observed from

257 on transport, sizeable band gaps and ease of hybridisation, they are set to become a versatile tool t

258 formation of stable double-stranded DNA, by hybridisation to a complementary sequence.

259 e used per locus and each tag is detected by hybridisation to a concatameric DNA probe labelled with

260 es, adult liver and kidney, were compared by hybridisation to a set of cDNA microarrays containing 65

261 is tailed amplicon facilitates detection via hybridisation to a surface immobilised oligonucleotide c

262 trodes of a genosensor array, and subsequent hybridisation to an enzyme labelled reporter probe.

263 tigate this possibility, we utilised in situ hybridisation to determine the effect of energetic chall

264 No hybridisation to DNA from other species was observed, su

265 We used fluid percussion injury and in situ hybridisation to evaluate the expression of NGF mRNA in

266 vered and amplified quantitatively following hybridisation to genomic DNA.

267 tokeratin 13 along with fluorescence in-situ hybridisation to identify Y-chromosome positive buccal e

268 ave used orthotopic quail grafts and in situ hybridisation to investigate the long-term fate of rhomb

269 We used fluorescence in-situ hybridisation to label X and Y chromosomes.

270 ean telomere length was calculated by in-gel hybridisation to leucocyte DNA from 56 normal individual

271 We have used the technique of in situ hybridisation to localise this receptor subunit to the l

272 omics, mouse transgenic analysis and in-situ hybridisation to predict and validate the existence of a

273 tients were assessed by fluorescence in situ hybridisation to quantify NTHi presence.

274 e nature of mRFP transcripts, we use in situ hybridisation to reveal the dynamic spatio-temporal patt

275 n less than 5 min at 37 degrees C via direct hybridisation to short probes immobilised on individual

276 ied the emerging technique of tissue in situ hybridisation to the analysis of the Drosophila segmenta

277 ocatalytic PtNPs were then used for covalent hybridisation to the PSA modified electrode and then app

278 Using in situ hybridisation to tissue sections, we have shown that the

279 Using in situ hybridisation to visualise sites of active her1 transcri

280 be a large-scale investigation of microarray hybridisations to murine probes with known sequence mism

281 and mating success, resulting in asymmetric hybridisation upon secondary contact.

282 Oxytocin mRNA was also measured by in situ hybridisation using a 3H- or 35S-labelled oligonucleotid

283 Fluorescence in situ hybridisation using chromosome specific probes identifie

284 human eye development, we performed in situ hybridisation utilising human embryonic tissue, and obse

285 belled oligonucleotide probe against exon C: hybridisation was seen over the cytoplasm of supraoptic

286 Using mRNA in situ hybridisation, we show here that ecdysone triggers the u

287 conjunction with double-fluorescent in situ hybridisation, we show that, at the beginning of neurula

288 nd cytogenetic analysis with genomic in situ hybridisation were applied to identify alien chromatin i

289 lation on the kinetics and thermodynamics of hybridisation were measured by comparing a fully methyla

290 microscopy, RNA-Seq analyses and RNA in situ hybridisation were used to compare three species, two (w

291 of the duplex, which can be used for direct hybridisation with a surface immobilised probe and an en

292 orld have revealed the presence of extensive hybridisation with both native and other introduced taxa

293 e lower level of secondary structure allowed hybridisation with complementary probes made with natura

294 ic species often maintain a certain level of hybridisation with their closest sexual relatives, poten

295 Fluorescence in situ hybridisation with these cosmids was used to refine the

296 entiate there without fusion, we did in-situ hybridisation with Y and X chromosome probes labelled wi

297 Hybridisations with a set of three independent blackfly

298 Polyploidy and interspecific hybridisation (with which it is often associated) have l

299 t artificial chromosome-fluorescence in situ hybridisation (YAC-FISH) mapped the WNT7A gene to chromo

300 vable and stable linkers, giving the highest hybridisation yields for surfaces containing approximate