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1 protein that is monomeric, well-folded, and hyperthermophilic.
2 study demonstrates coenzyme engineering of a hyperthermophilic 6PGDH and its application to high-temp
3 s islandicus rod-shaped virus 2) infects the hyperthermophilic acidophile Sulfolobus islandicus, whic
4 aracterized an archaeal antioxidant from the hyperthermophilic acidophile Sulfolobus solfataricus.
6 l mobilities differ between a mesophilic and hyperthermophilic adenylate kinase, but are strikingly s
12 ogue (TP0823) for neelaredoxin, an enzyme of hyperthermophilic and sulfate-reducing anaerobes shown t
13 nt comparison of ECAK dynamics with those of hyperthermophilic Aquifex aeolicus AK (AAAK), our result
14 ) accumulates as a compatible solute in many hyperthermophilic archaea (e.g., Archaeoglobus fulgidus)
15 riginated in an extreme environment, such as hyperthermophilic archaea (Pyrococcus furiosus), are sig
16 RNA-encoding DNA analysis places many of the hyperthermophilic Archaea (species with an optimum growt
17 f structures and complete genomes of several hyperthermophilic archaea and bacteria revealed that org
18 bilizes tRNAs from thermophilic bacteria and hyperthermophilic archaea and is required for growth at
19 interaction with uracil is not restricted to hyperthermophilic archaea and that the polymerase from m
20 from common yeasts to extremophiles such as hyperthermophilic archaea can also generate high current
24 In particular, the approaches employed by hyperthermophilic archaea have been a general source of
25 f the DNA replication-associated proteins of hyperthermophilic archaea have yielded considerable insi
26 Inositol monophosphatase (EC 3.1.3.25) in hyperthermophilic archaea is thought to play a role in t
29 rs contained in a microbiome-associated with hyperthermophilic archaea of the order Sulfolobales reco
31 ficity and binding mechanism of MCM from the hyperthermophilic Archaea Sulfolobus solfataricus on var
34 quences (ISs) are abundant and widespread in hyperthermophilic archaea, but few experimental studies
35 e that the intracellular proteins of certain hyperthermophilic archaea, especially the crenarchaea Py
37 d over a contiguous 16 kb region between two hyperthermophilic Archaea, Pyrococcus furiosus and Therm
38 esponses have been of particular interest in hyperthermophilic archaea, since these microbes live und
39 s of recombination involving short ssDNAs in hyperthermophilic archaea, we evaluated oligonucleotide-
48 7d are two small chromatin proteins from the hyperthermophilic archaeabacterium Sulfolobus solfataric
50 Moreover, this latest example of a split hyperthermophilic archaeal DNA polymerase further illust
51 els for dNTP, ddNTP, and acyNTP selection by hyperthermophilic archaeal DNA polymerases to rationaliz
52 the parameters for dNTP incorporation by the hyperthermophilic archaeal Family B Vent DNA polymerase
53 trand RNA viruses that probably replicate in hyperthermophilic archaeal hosts and are highly divergen
55 Analysis of the genome sequence of the small hyperthermophilic archaeal parasite Nanoarchaeum equitan
57 first structure of a catalytic domain from a hyperthermophilic archaeal viral integrase reveals a min
61 a voltage-dependent K+ (K(V)) channel from a hyperthermophilic archaebacterium from an oceanic therma
62 structure of adenylosuccinate lyase from the hyperthermophilic archaebacterium Pyrobaculum aerophilum
63 is work, we characterize the enzyme from the hyperthermophilic archaebacterium Pyrococcus furiosus.
66 m chain alcohol dehydrogenase (ADH) from the hyperthermophilic archaeon Aeropyrum pernix has been sol
67 he Aeropyrum coil-shaped virus (ACV), of the hyperthermophilic archaeon Aeropyrum pernix, with a viri
69 A new carboxyl esterase, AF-Est2, from the hyperthermophilic archaeon Archaeoglobus fulgidus has be
71 structure of the 104 residue SRP19 from the hyperthermophilic archaeon Archaeoglobus fulgidus, desig
74 ctures, to our knowledge, of an ACD from the hyperthermophilic archaeon Candidatus Korachaeum cryptof
75 Sulfolobus acidocaldarius is so far the only hyperthermophilic archaeon in which genetic recombinatio
83 lectron transfer flavoprotein (ETF) from the hyperthermophilic archaeon Pyrobaculum aerophilum The Et
86 an endonuclease III homolog, PaNth, from the hyperthermophilic archaeon Pyrobaculum aerophilum, whose
87 ation of a putative DNA glycosylase from the hyperthermophilic archaeon Pyrobaculum aerophilum, whose
92 transcarbamoylase (OTCase) from the deep sea hyperthermophilic archaeon Pyrococcus abyssi demonstrate
93 ned the solution structure of RPP21 from the hyperthermophilic archaeon Pyrococcus furiosus ( Pfu) us
94 ngle cubane cluster ferredoxin (Fd) from the hyperthermophilic archaeon Pyrococcus furiosus (Pf) have
95 echanism of the H(+)-dependent MATE from the hyperthermophilic archaeon Pyrococcus furiosus (PfMATE).
96 es from Escherichia coli (EcMetAP-I) and the hyperthermophilic archaeon Pyrococcus furiosus (PfMetAP-
97 ng for the methionyl aminopeptidase from the hyperthermophilic archaeon Pyrococcus furiosus (PfMetAP-
98 tic studies were conducted on the POP of the hyperthermophilic archaeon Pyrococcus furiosus (Pfu) 85
99 protons of perdeuterated rubredoxin from the hyperthermophilic archaeon Pyrococcus furiosus and the m
101 ylase was identified in cell extracts of the hyperthermophilic archaeon Pyrococcus furiosus by its ab
103 shed membrane preparations from cells of the hyperthermophilic archaeon Pyrococcus furiosus contain h
108 The cytoplasmic hydrogenase (SHI) of the hyperthermophilic archaeon Pyrococcus furiosus is an NAD
109 ss of PfMATE, a proton-coupled MATE from the hyperthermophilic archaeon Pyrococcus furiosus Pairs of
118 A intein located in the ATPase domain of the hyperthermophilic archaeon Pyrococcus horikoshii is stro
119 domain of life, with the discovery that the hyperthermophilic archaeon Sulfolobus has three replicat
120 II chaperonins known as rosettasomes in the hyperthermophilic archaeon Sulfolobus shibatae, are not
124 ce of a global gene regulatory system in the hyperthermophilic archaeon Sulfolobus solfataricus is de
125 ral modules of the homomultimeric MCM of the hyperthermophilic archaeon Sulfolobus solfataricus.
126 in chromatin structure and regulation in the hyperthermophilic archaeon Sulfolobus solfataricus.
127 noncatalytic subunit, denoted PriX, from the hyperthermophilic archaeon Sulfolobus solfataricus.
128 eavage of mRNA from an invading virus in the hyperthermophilic archaeon Sulfolobus solfataricus.
129 gh mutagenesis of the Sso7d protein from the hyperthermophilic archaeon Sulfolobus solfataricus.
130 gh mutagenesis of the Sso7d protein from the hyperthermophilic archaeon Sulfolobus solfataricus; Sso7
132 structure of TIM from Thermoproteus tenax, a hyperthermophilic archaeon that has an optimum growth te
133 cture of the ribosomal protein L30e from the hyperthermophilic archaeon Thermococcus celer determined
134 that ribonucleotides are incorporated by the hyperthermophilic archaeon Thermococcus kodakarensis bot
138 ted Box C/D RNAs from Pyrococcus furiosus, a hyperthermophilic archaeon, into the nuclei of oocytes f
141 ily DNA replication polymerase (Dpo1) in the hyperthermophilic archaeon, Sulfolobus solfataricus, is
147 aracterized an unusual type IA enzyme from a hyperthermophilic archaeum, Nanoarchaeum equitans, which
150 methanarchaeon, Methanococcus jannaschii, a hyperthermophilic, autotrophic, and strictly hydrogenotr
153 existence of peptide-based quorum sensing in hyperthermophilic bacteria and indicate that cellular co
154 .4 A resolution, of the class I BPL from the hyperthermophilic bacteria Aquifex aeolicus (AaBPL) in i
155 ins taken from mesophilic, thermophilic, and hyperthermophilic bacteria are studied using these metho
157 Our results explain the root location of hyperthermophilic bacteria in the phylogenetic tree for
161 onate-8-phosphate synthase (KDO8PS) from the hyperthermophilic bacterium Aquifex aeolicus differs fro
163 tural features of the NtrC1 protein from the hyperthermophilic bacterium Aquifex aeolicus suggested t
164 An NAD(+)-dependent DNA ligase from the hyperthermophilic bacterium Aquifex aeolicus was cloned,
165 rt the crystal structures of GatCAB from the hyperthermophilic bacterium Aquifex aeolicus, complexed
166 he crystal structure of a homologue from the hyperthermophilic bacterium Aquifex aeolicus, that share
172 unknown type of protein-only RNase P in the hyperthermophilic bacterium Aquifex aeolicus: Without an
173 is of the crystal structure of MurI from the hyperthermophilic bacterium Aquifex pyrophilus, we perfo
174 us ribose-binding protein, isolated from the hyperthermophilic bacterium Thermoanaerobacter tengconge
175 RAP-PBP (open reading frame tm0322) from the hyperthermophilic bacterium Thermotoga maritima (TM0322)
176 define how the type III-B effector from the hyperthermophilic bacterium Thermotoga maritima discrimi
177 hosphogluconate dehydrogenase (6PGDH) from a hyperthermophilic bacterium Thermotoga maritima from its
181 ide expression patterns during growth of the hyperthermophilic bacterium Thermotoga maritima on 14 mo
182 eterodimeric ABC exporter TM287/288 from the hyperthermophilic bacterium Thermotoga maritima using al
183 The biochemical behavior of RNase III of the hyperthermophilic bacterium Thermotoga maritima was anal
184 The structure of RNase P protein from the hyperthermophilic bacterium Thermotoga maritima was dete
185 of proteins from one specific organism, the hyperthermophilic bacterium Thermotoga maritima, and tho
191 tandemly repeated GTP-binding domains from a hyperthermophilic bacterium, Thermotoga maritima, was cl
197 nstrate that the Thermoproteales, a clade of hyperthermophilic Crenarchaea, lack a canonical SSB.
200 es or genes that are first in operons in the hyperthermophilic crenarchaeon P. aerophilum proceeds mo
201 tion crystal structure of SurEalpha from the hyperthermophilic crenarchaeon Pyrobaculum aerophilum (P
202 and a PCNA homolog (Pa-PCNA1), both from the hyperthermophilic crenarchaeon Pyrobaculum aerophilum (T
203 ionine beta-synthase domain protein from the hyperthermophilic crenarchaeon Pyrobaculum aerophilum.
204 h10b, a member of the Sac10b family from the hyperthermophilic crenarchaeon Sulfolobus shibatae, bind
205 erial-like DnaG primase contained within the hyperthermophilic crenarchaeon Sulfolobus solfataricus (
207 erase-Like Lactonases (PLLs) family from the hyperthermophilic crenarchaeon Vulcanisaeta moutnovskia
209 ea, and an activity has been observed in the hyperthermophilic crenarchaeote Sulfolobus solfataricus.
211 architecture of this protein is uncommon for hyperthermophilic endoglucanases, and two of the four do
213 or directly assaying the activity of another hyperthermophilic enzyme, 1,4-beta-D-glucan glucohydrola
215 ut the substrate specificity of two specific hyperthermophilic enzymes and the first test of some nat
225 p in CoM biosynthesis, was identified in the hyperthermophilic euryarchaeon Methanococcus jannaschii.
228 nd molecular screens, although abundances of hyperthermophilic heterotrophs were relatively high.
230 discovered IgnaviCas9, a Cas9 protein from a hyperthermophilic Ignavibacterium identified through min
231 essure, which is significantly different for hyperthermophilic (IPPase) and mesophilic (HEWL) protein
233 sitions -40 to +1 of the gdh promoter of the hyperthermophilic marine archaea, Pyrococcus furiosus (P
234 s of this family have been identified in the hyperthermophilic marine archaeon Methanococcus jannasch
235 ha family (family B) DNA polymerase from the hyperthermophilic marine archaeon Thermococcus sp. 9 deg
236 of Thermofilum pendens, a deeply branching, hyperthermophilic member of the order Thermoproteales in
240 To our knowledge, Amt proteins are the first hyperthermophilic membrane transport proteins shown to b
241 d stability from the hot-start toward modern hyperthermophilic, mesophilic, and psychrophilic organis
242 thanocaldococcus jannaschii--a deeply rooted hyperthermophilic methanogen growing only on H2 plus CO2
243 first structures of archaeal G1PDH from the hyperthermophilic methanogen Methanocaldococcus jannasch
245 have been detected in a non-nitrogen-fixing hyperthermophilic methanogen, Methanocaldococcus jannasc
246 ata support our estimated H(2) threshold for hyperthermophilic methanogenesis at vents and highlight
247 pure culture H(2) threshold measurements for hyperthermophilic methanogenesis in low-temperature hydr
249 of genome sequence data from mesophilic and hyperthermophilic micro-organisms has revealed a strong
251 is a large oligomeric protein derived from a hyperthermophilic microorganism that is found near hydro
252 aled that, on a global scale, populations of hyperthermophilic microorganisms are isolated from one a
253 redicted to be particularly energy-rich, and hyperthermophilic microorganisms that broadly reflect su
255 The family 4 uracil-DNA glycosylase from the hyperthermophilic organism Archaeoglobus fulgidus (AFUDG
262 roteins have been characterized in extremely hyperthermophilic organisms, and most function as repres
263 ungsten, which substitutes for molybdenum in hyperthermophilic organisms, could also be ligated to mo
267 sing DNA composition bias in genomes of some hyperthermophilic organisms: simply screening for GC-ric
269 used thioredoxin (Trx) folds, belongs to the hyperthermophilic protein disulfide oxidoreductase famil
270 n enzymatically active, soluble variant of a hyperthermophilic protein that is normally insoluble whe
271 utions to the folding free energy of several hyperthermophilic proteins and their mesophilic homologs
272 aken together, our results suggest that many hyperthermophilic proteins enhance electrostatic interac
273 o 100 degrees C emphasizes the importance in hyperthermophilic proteins of the specific location of i
274 tabilizing forces required for extracellular hyperthermophilic proteins to tolerate high-temperature
275 of improving the low-temperature activity of hyperthermophilic proteins, likely by facilitating the i
276 in 1998, the method has been used to create hyperthermophilic proteins, to evolve novel folded domai
280 ys+1, in an intein precursor composed of the hyperthermophilic Pyrococcus abyssi PolII intein and ext
281 We report the solution NMR structures of the hyperthermophilic Pyrococcus abyssi PolII intein, which
282 idium pasteurianum (Topt = 37 degrees C) and hyperthermophilic Pyrococcus furiosus (Topt = 95 degrees
285 g agent, dextran 20, on the folded states of hyperthermophilic (S16Thermo) and mesophilic (S16Meso) h
286 coli and WrbA from Archaeoglobus fulgidus, a hyperthermophilic species from the Archaea domain, shows
288 d refined the structure of the mIPS from the hyperthermophilic sulfate reducer Archaeoglobus fulgidus
289 (diaphorase) activity was isolated from the hyperthermophilic sulfate-reducing anaerobe Archaeoglobu
290 inantly expressed components of SRP from the hyperthermophilic, sulfate-reducing archaeon Archaeoglob
291 onlytic temperate viruses were isolated from hyperthermophilic Sulfolobus hosts, and both viruses sha
292 loned and purified the RadA protein from the hyperthermophilic, sulphate-reducing archaeon Archaeoglo
293 ior previously observed for thermophilic and hyperthermophilic superoxide dismutases but over a lower
294 um, a facultatively aerobic nitrate-reducing hyperthermophilic (T(opt) = 100 degrees C) crenarchaeon.
296 , it has been suggested that thermophilic or hyperthermophilic (Tm) enzymes have lower catalytic powe
298 scuous natural human IgG-binding domain, the hyperthermophilic variant of protein G (HTB1), into a hi
299 , single-point core mutants of a 57-residue, hyperthermophilic variant of the B1 domain of protein G