ライフサイエンスコーパス: imino proton

1 One of these involves a rapidly exchanging G imino proton.

2 lacking due to the rapid exchange of the U23 imino proton.

3 Imino proton and phosphorus NMR spectra suggest that som

4 intensities of the Watson-Crick base-paired imino protons and a reduction by 20 degrees C in the upp

5 Upfield chemical shifts of duplex imino protons and the disruption of the NOE base-sugar c

6 Psi has two hydrogen donors (N1 and N3 imino protons) and two hydrogen bond acceptors because t

7 rbance melting curves, NMR titrations of the imino protons, and analysis of NMR spectra in the presen

8 The exchange times for Watson-Crick imino protons are approximately 1 h for both triplexes.

9 The exchange kinetics of the imino protons are measured from selective longitudinal r

10 Using NOESY, the imino protons as well as the cytosine amino protons of a

11 All imino protons associated with the iG:C, G:iC, and A:T (e

12 the selectively enhanced exchange rates for imino protons associated with these base pairs.

13 ls due to protons on carbon 2 of adenine and imino protons at the central five A-T pairs of the 11 ba

14 sequences, the solvent exchange rates of the imino protons at the junction of the helical stems in th

15 , as well as the upfield shifts observed for imino protons at this step, serve to define the central

16 o duplex as evident from the presence of the imino proton (at 13.70 ppm) of an A:T base pair.

17 d the U8-H6 and U8-H5, thus orienting the U8 imino proton away from U5.

18 Increasing the pK(a) value of the imino proton by reduction of its 5,6-double bond results

19 onuclear correlations involving RNA's labile imino protons can be significantly enhanced, by exploiti

20 d of Mg2+to P5 were determined by monitoring imino proton chemical shifts during titration of the RNA

21 the dramatic change that IHF imposes on the imino proton chemical shifts is indicative of a severe d

22 Analysis of imino proton chemical shifts show that structural pertur

23 rbations at the binding-site as reflected in imino proton complexation shifts and specific altered su

24 ructure models that are indistinguishable in imino proton connectivities.

25 e calculated values of the exchange rates of imino protons, especially those obtained from measuremen

26 ed the CLEANEX-PM NMR protocol to detect DNA imino proton exchange and analyzed the dynamics of oxoG:

27 r in the two structures are characterized by imino proton exchange and nuclear magnetic resonance spe

28 The kinetics of imino proton exchange as a function of basic pH or added

29 opening rates in RNA are consistent with the imino proton exchange experiments for AU base pairs, alt

30 shows that nuclear magnetic resonance-based imino proton exchange is a good probe for detection of w

31 Imino proton exchange measurements also yielded the diss

32 -terminal bases were determined by measuring imino proton exchange rates as a function of exchange ca

33 Measurements of ligand amide and DNA imino proton exchange rates indicate very slow dissociat

34 xternal catalysis exchange mechanisms on the imino proton exchange rates is analyzed.

35 characterizing the temperature dependence of imino proton exchange rates of individual basepairs.

36 perature dependence of the UV absorption and imino proton exchange rates provides insight into the st

37 base pair opening parameters estimated from imino proton exchange rates suggest that the stability o

38 eactive base-paired nucleotides exhibit high imino proton exchange rates.

39 ches derived from 1H NMR measurements of the imino proton exchange rates.

40 spectroscopy and by measuring base-catalyzed imino proton exchange rates.

41 appear to close more slowly than the rate of imino proton exchange with bulk water, since their excha

42 to characterize the effects of a catalyst of imino proton exchange, namely, ammonia upon the structur

43 This is surprising, given that imino-proton exchange rates show that basepairs in a DNA

44 ntration, the exchange times of the mismatch imino protons extrapolate to much shorter lifetimes than

45 TAR allowed observation of the C23 amino and imino protons for the first time, providing direct evide

46 pening at X(6).T(17) and protected the T(17) imino proton from solvent exchange.

47 The imino protons from the different mismatched, as well as

48 lly greater than that of the corresponding T imino proton; however, this difference is not attributed

49 The exchange characteristics of the imino protons identify the existence of four additional

50 We have measured the exchange rates of imino protons in each triad of the three triplexes using

51 The results indicate that the exchange of imino protons in protonated cytosines is most likely lim

52 as the solvent accessibility of the unpaired imino protons in the complex.

53 nge of individual Watson-Crick and Hoogsteen imino protons in the DNA triple helix were measured in t

54 parative studies of hydrogen exchange of the imino protons in the free RNA aptamer and the AMP-RNA ap

55 ic reduction in exchange is observed for the imino protons in the IHF bound DNA.

56 aracteristics of the extremely well resolved imino protons in the NMR spectrum of the complex.

57 e pair opening were measured for most of the imino protons in the P1 duplex using the base catalysts

58 exchange cross peaks for several base-paired imino protons in the RNA yielded an apparent k(on) of 60

59 that Mg2+ lowers the exchange rates of most imino protons in the structure by stabilizing the corres

60 re the exchange rate constant (k(ex)) of the imino protons in the unbound, cocaine-bound, and quinine

61 The exchange rates of imino protons in this triple helix have been measured by

62 the triplex structure, the exchange rates of imino protons in Watson-Crick base pairs are up to 5000-

63 The downfield shift of the 3'-G imino protons indicates the complex makes hydrogen bond

64 n NMR spectrum shows resonances expected for imino protons involved in guanine quartet base-pairing.

65 nternal Psi-A, Psi-G and Psi-U pairs, the N3 imino proton is hydrogen bonded to the opposite strand n

66 NMR studies of imino proton lifetime, solvent accessibility, and NOE co

67 to the opposite strand nucleotide and the N1 imino proton may also be hydrogen bonded.

68 One-dimensional imino proton NMR and circular dichroism spectra of the m

69 Imino proton NMR data provided evidence that elements of

70 Ribonuclease mapping of T4-32 and imino proton NMR experiments of T4-35 show that both seq

71 Imino proton NMR experiments provide evidence for possib

72 Imino proton NMR indicates that this is partly because t

73 For selected sequences, imino proton NMR provides evidence that the desired dupl

74 Imino proton NMR spectra were also measured for two syst

75 UV and imino proton NMR spectral observations indicated pronoun

76 The temperature-dependent imino proton NMR spectrum of oxoG modified DNA confirms

77 lts from UV experiments were corroborated by imino proton NMR studies that show proton exchange rates

78 studied by optical melting, one-dimensional imino proton NMR, and one-dimensional phosphorus NMR.

79 oresis, equilibrium ultracentrifugation, and imino proton NMR, we are able to show that these modific

80 nternal loops were studied by UV melting and imino proton NMR.

81 m mismatches, were studied by UV melting and imino proton NMR.

82 e versus temperature melting experiments and imino proton nuclear magnetic resonance (NMR).

83 The observed imino proton nuclear magnetic resonance resonances and F

84 nd carbonyl oxygen of G, another between the imino proton of G and carbonyl oxygen O2 of U.

85 largest chemical shift change occurred at an imino proton of one of the G.A base-pairs, no nuclear Ov

86 ed in 95% H(2)O, 5% D(2)O indicated that the imino proton of the base opposite N14, G5, or T5, formed

87 The line width of the imino proton of the ClU residue is substantially greater

88 eased level of chemical exchange for the ClU imino proton of the ClU-A base pair, the ClU residue is

89 The imino proton of the lesion-containing base pair resonate

90 ective changes to the chemical shifts of the imino protons of a GCGA tetraloop in the 75mer, that is

91 at pressure disrupts the interactions of the imino protons of the uridine and guanosine U-A and G-C b

92 imensional NOE data show no NOEs between the imino protons of U5 and U8, but NOEs are observed betwee

93 Functional group substitution shows that the imino proton on the N1 is critical, suggesting a possibl

94 from the dependence of the exchange rates of imino protons on ammonia concentration.

95 The imino proton region of 1-D NMR spectra shows that G.G an

96 One-dimensional (1)H NMR spectra of the imino proton region of I in the presence of this series

97 Observation of an imino proton resonance at 15.6 p.p.m. provides evidence

98 he 1.13 ppm upfield shift of the thioguanine imino proton resonance, and the large increase in the ex

99 Analysis of imino proton resonances and NOEs indicates additional en

100 ding affinity was measured by monitoring RNA imino proton resonances for some of the compounds that s

101 by pronounced upfield shifts of the G-tetrad imino proton resonances in the NMR, which is similar to

102 Imino proton resonances indicate the hydrogen bonding pa

103 addition, the temperature dependence of the imino proton resonances is also consistent with the diff

104 clear NMR experiments aided in assigning the imino proton resonances of the DNA alone and in complex

105 The assignment of the imino proton resonances with characteristic chemical shi

106 The temperature dependence of the imino proton resonances, as well as UV absorption data,

107 ive imaging agents, since their exchangeable imino protons resonate at 5-6 ppm from the water proton

108 rees C in the upper temperature at which the imino proton signals are detectable, consistent with des

109 The N3-nitrogen and imino proton signals of an (15)N-labeled thymine dimer-c

110 nding interface was obtained from changes in imino proton signals of uniformly 15N-labeled Tar with i

111 Thermal denaturation and imino proton solvent exchange experiments reveal that th

112 and the exchange rates of the corresponding imino protons, suggest that these two basepairs open in

113 The downfield shift of the G8 imino proton supports the conclusion that the pendant gu

114 Chemical shift changes of the imino protons upon titration of the RNA hairpin with Mg(

115 d from measurements of the exchange rates of imino protons using nuclear magnetic resonance spectrosc

116 However, the expected U23 imino proton was not observed, preventing unambiguous id

117 The resonance arising from the T(17) imino proton was not observed.

118 rates of exchange for the slowest exchanging imino protons were approximately 20 times faster in unmo

119 NOE connectivities between imino protons were disrupted at T16 and T17.

120 ar set of slowly exchanging (t(1/2) > 3 min) imino protons were observed in both tRNAs, but the rates

121 OESY WATERGATE spectra show an NOE between U imino protons, which suggests that U4 and U9 form a hydr

122 ease in the exchange rate of the thioguanine imino proton with water.

123 The rates of exchange of imino protons with solvent in the two structures have be

124 erized by measuring the rates of exchange of imino protons with solvent protons as a function of the

125 The exchange of imino protons with solvent protons was measured for each

126 The rates of exchange of imino protons with solvent protons were measured by NMR

127 complex with IHF, the exchange rates of the imino protons with the solvent have been measured for H1

128 pon measurements of the rates of exchange of imino protons with water protons at high concentrations