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1 ethods based on biological properties (e.g., immunoaffinity).
2 n 70 (HSP70) IgG was isolated from plasma by immunoaffinity.
3 GFR protein (CD271, p75NTR) were isolated by immunoaffinity adsorption, and cultured as a monolayer f
8 f patients with pancreatic cancer, including immunoaffinity and label-free physical attribute-based c
10 f >10 mg of monomeric hOR17-4 receptor after immunoaffinity and size exclusion chromatography, with e
11 eled CCR5, which was purified using a tandem immunoaffinity and size-exclusion chromatography approac
16 observations demonstrate the utility of CD45 immunoaffinity-based approaches for producing highly pur
17 rochannel and also a technique for improving immunoaffinity-based circulating tumor cell (CTC) captur
24 as isolated through binding of either tag to immunoaffinity beads and then decorated with antibodies
26 cow's milk allergy has been performed using immunoaffinity capillary electrophoresis (IACE) coupled
29 say that uses sequential protein and peptide immunoaffinity capture for protein target quantitation.
32 nd AhR knockdown H358 cells was validated by immunoaffinity capture stable isotope dilution ([(15)N(5
33 on density gradient fractionation and direct immunoaffinity capture to precisely characterize the RNA
34 t-generation disulfide-linked ADCs involving immunoaffinity capture, chemical cleavage, and LC-MS/MS.
38 fied ISGylated proteins from human thymus by immunoaffinity chromatography and analyzed ISG15 conjuga
39 ctrometry (MS/MS) assay that utilizes online immunoaffinity chromatography and column switching was d
40 , we isolated apo(a)-containing particles by immunoaffinity chromatography and determined the concent
41 ted complexes containing PLTP from plasma by immunoaffinity chromatography and determined their compo
43 he intervention measured by using sequential immunoaffinity chromatography and liquid chromatography-
45 f serpin-6 and PAP-3 in induced hemolymph by immunoaffinity chromatography and mass spectrometry.
48 300 gel filtration chromatography, anti-FLAG immunoaffinity chromatography and SDS/PAGE permitted pur
50 zed receptor was easily purified by one-step immunoaffinity chromatography and the purified receptor
52 as separated by anti-apoC-III and anti-apoE immunoaffinity chromatography and ultracentrifugation in
53 d several mutants, from transfected cells by immunoaffinity chromatography and visualized individual
54 say methods were developed for detection and immunoaffinity chromatography capture was developed for
56 C heterocomplex was isolated and purified by immunoaffinity chromatography from insect cells co-expre
57 is mAb, isolation of the troponin complex by immunoaffinity chromatography from muscle homogenate and
58 hain, cross-linked peptides were isolated by immunoaffinity chromatography from proteolytic digests o
59 from rod outer segments of bovine retinae by immunoaffinity chromatography in octyl glucoside was rec
62 s of the protein to facilitate separation by immunoaffinity chromatography of overproduced mutant enz
63 e (EEF) tripepetide to allow purification by immunoaffinity chromatography on an immobilized monoclon
67 extracts by a combination of traditional and immunoaffinity chromatography steps and found that the p
69 olution mass spectrometry (MS) combined with immunoaffinity chromatography to determine quantitativel
70 nt signaling, we used cell fractionation and immunoaffinity chromatography to examine the physical en
72 o identify new vaccine targets, we have used immunoaffinity chromatography to isolate class I HLA-A*0
73 pump-generated flow to deliver reagents and immunoaffinity chromatography to isolate the antigen fro
75 nds the powerful technique of gentle-release immunoaffinity chromatography to many expressed proteins
77 control children (n = 54), we used recycling immunoaffinity chromatography to measure the neuropeptid
78 f conventional chromatography and anti-SRCAP immunoaffinity chromatography to purify a native human S
79 and HBeAg, we used these Fabs in microscale immunoaffinity chromatography to purify HBeAg from indiv
80 urified from transformed Escherichia coli by immunoaffinity chromatography using a monoclonal antibod
86 ern blot analyses, immunohistochemistry, and immunoaffinity chromatography were used to detect and pu
87 compartments were seen following pulldown by immunoaffinity chromatography with Rab-specific antibodi
88 roteases inhibited by these serpins, we used immunoaffinity chromatography with serpin antibodies to
89 ication procedure (size, heparin, anion, and immunoaffinity chromatography): two heat shock proteins
92 e unlabeled apoB:1000 particles, isolated by immunoaffinity chromatography, contained 56 PL, 8 TAG, a
93 usly pure mSR-BI-t1, prepared by single-step immunoaffinity chromatography, mediated high affinity HD
96 n of endogenous levels of (FLAG)betabeta' by immunoaffinity chromatography, which was found to have 0
97 lecules from human plasma by on-line coupled immunoaffinity chromatography-asymmetric flow field-flow
117 treatment and centrifugation, followed by an immunoaffinity column (IAC) clean-up step before mass sp
119 liquid-liquid extraction, clean-up using an immunoaffinity column (IAC), and identification by rever
120 the types of antibodies that are used in the immunoaffinity column and by using an appropriate detect
121 n in bronchoalveolar lavage and an anti-SNIP immunoaffinity column binds a 70-kDa protein in U937 cel
124 fects of human Abs affinity-purified (AF) by immunoaffinity column chromotography on excitation-contr
126 munoadsorption (EIA) of anti-Gal Ab using an immunoaffinity column of a Gal type 6 oligosaccharide de
127 ter two-dimensional gel electrophoresis, and immunoaffinity column purification, N-terminal amino-aci
132 S method against sample preparation using an immunoaffinity column; the recovery and specificity were
133 Ochratoxin A analyses were performed with immunoaffinity columns and detection by high performance
134 gel filtration and associated with RNAPII on immunoaffinity columns prepared with anti-CTD antibodies
135 C instrumentation after pre-separation using immunoaffinity columns that work through a mechanism of
136 native cleanup procedures (MultiSep and IAC--immunoaffinity columns) was performed, being the advanta
141 observation, we have developed a CD45-based immunoaffinity depletion method for removing CD45-contai
144 ll reproducibility of the process, including immunoaffinity depletion, is high, with a process replic
149 inase ubiquitination using ubiquitin remnant immunoaffinity enrichment and quantitative mass spectrom
153 e methods to characterize the performance of immunoaffinity enrichment of peptides up to multiplex le
154 tryptic digestion of PAPs in suspension, an immunoaffinity enrichment of peptides, and LC-MS/MS-base
155 DAMTS-13 substrate (vWFh) was performed with immunoaffinity enrichment of the reaction substrate and
156 The assay combines magnetic bead-based NGF immunoaffinity enrichment using a non-neutralizing polyc
158 gh-resolution mass spectrometry coupled with immunoaffinity enrichment, we identified 47 lysine-acety
159 alytes and solutions to carry out integrated immunoaffinity extraction and electrophoretic separation
164 whole spot was dissolved prior to cleanup by immunoaffinity extraction, tryptic digest, and preconcen
167 mated using the VICAM AflaTest and OchraTest immunoaffinity fluorometric method in a total of 50 meat
170 s strain behaved as a typical BoNT/B, having immunoaffinity for anti-B monoclonal antibodies and clea
171 rofiltration into a microfluidic device with immunoaffinity for enhanced capture efficiency of CTCs.
172 from the liver was measured with a sensitive immunoaffinity/gas chromatography/high-resolution mass s
173 is presented, which involves coextraction by immunoaffinity (IA) beads and codetermination by selecte
174 pectrometry (LC/MS/MS) typically utilizes an immunoaffinity (IA) enrichment step such as immunoprecip
177 or the CTC-based cancer prognosis, relies on immunoaffinity interactions between CTCs and antibodies
178 chromatography/mass spectrometry analysis of immunoaffinity isolates of hippocampal neurons, that RAR
180 lial cell line with C muridarum, followed by immunoaffinity isolation and sequencing of MHC class I-
182 ion of a pronase digestion step prior to the immunoaffinity LC-MS/MS allowed for measuring not only f
186 tadiene for 90 days, using a newly developed immunoaffinity liquid chromatography tandem mass spectro
196 of 180 ms between the sample and an anti-BSA immunoaffinity microcolumn and provided a signal within
200 we demonstrate using a combination of online immunoaffinity-postconcentration-mass spectrometry in co
201 ic target for HSV-1 infection, and a protein immunoaffinity procedure, we enriched fully glycosylated
203 , we used a discovery platform consisting of immunoaffinity profiling coupled to mass spectrometry th
204 tive phosphorylation of STAT5 and applied an immunoaffinity profiling strategy to identify tyrosine-p
205 can be recognized by specific antibodies for immunoaffinity purification (IAP) and subsequent identif
207 elles containing TrkB isolated from brain by immunoaffinity purification also contain dynein with IC-
208 eract with Kir2 channels, as demonstrated by immunoaffinity purification and affinity chromatography
214 loyed proteomic screening by phosphotyrosine immunoaffinity purification and tandem mass spectrometry
215 the A17 membrane protein was demonstrated by immunoaffinity purification and Western blot analysis.
219 um freudenreichii subsp. shermanii and after immunoaffinity purification in extracts of cereal matric
220 pecific recognition of PEGylated species, an immunoaffinity purification method (IAP) using anti-PEG
225 rategy that incorporates rapid and efficient immunoaffinity purification of Complex I followed by dif
226 tity of proteins analyzed by LC-MS following immunoaffinity purification of IsoLG-modified proteins.
227 -denaturing conditions has made possible the immunoaffinity purification of labile, multisubunit enzy
230 dividual serpin-1 isoforms in plasma we used immunoaffinity purification of serpin-1 isoforms from M.
232 we established that tcTPC allowed stringent immunoaffinity purification of the gamma-secretase compl
233 ed from the infected cells were subjected to immunoaffinity purification of the tagged proteins by ad
235 at represent 3 Bcr-Abl fusion types by using immunoaffinity purification of tyrosine phosphopeptides
236 d signaling intermediates were identified by immunoaffinity purification of tyrosine-phosphorylated p
237 undertaken by two independent approaches: 1) immunoaffinity purification on a mAb raised to condition
238 of 8-oxo-dGuo observed were confirmed by an immunoaffinity purification stable isotope dilution ([(1
240 ibody-induced hearing loss, we used antibody immunoaffinity purification to isolate the IESCA, which
243 cted human embryonal kidney (HEK) 293 cells, immunoaffinity purification, and mass spectrometry combi
244 d characterized by rate-zonal sedimentation, immunoaffinity purification, electron microscopy, and th
246 n tested for immunofluorescence staining and immunoaffinity purification, leading to putative identif
247 means of a bottom-up analytical approach via immunoaffinity purification, tryptic digestion, and subs
252 To investigate its function further, we immunoaffinity purified a bestrophin complex from RPE ly
253 LA-A*11:01 harvested from infected cells was immunoaffinity purified and acid boiled to release heavy
255 ble complex containing MLL1 and MOF has been immunoaffinity purified from a human cell line that stab
257 UDP-N-acetylglucosamine pyrophosphorylase immunoaffinity purified from encysting and non-encysting
260 d from six independent Mediator preparations immunoaffinity purified through their Nut2 (MED10), Med2
265 cDNA corresponding to the core protein of an immunoaffinity-purified arabinogalactan protein (AGP) se
266 rone assay after immunoprecipitation or with immunoaffinity-purified argpyrimidine-alpha-crystallin i
267 ugh acantholytic activities of each of these immunoaffinity-purified AuAbs could not induce a PV-like
270 was used to analyze proteins associated with immunoaffinity-purified HDL from plasma obtained from 2
272 es were exposed to various concentrations of immunoaffinity-purified leukotoxin and the cytotoxicity
275 dicate that OCP sera, OCP IgG fractions, and immunoaffinity-purified OCP autoantibodies react with th
276 m a keratinocyte expression library by using immunoaffinity-purified OCP autoantibody, encoded the cy
280 from the brain and lung extracts, and in the immunoaffinity-purified sample, but not in the negative
287 The method involved analyte enrichment by immunoaffinity separation using anti-rhEPO antibody link
288 mprised analyte extraction and enrichment by immunoaffinity separation with anti-rhEPO antibodies, du
289 Liquid-solid extraction, clean-up using immunoaffinity solid phase extraction chromatography, an
290 roduced in culture by first incorporating an immunoaffinity step using monoclonal antibodies to purif
295 purified the putative ATPDase from liver by immunoaffinity techniques and obtained a heavily glycosy
296 by density gradient ultracentrifugation and immunoaffinity techniques specific to apolipoprotein B-1
297 boratory to use LC/MS/MS in conjunction with immunoaffinity techniques to evaluate candidate biomarke
300 loped a novel workflow integrating DNA-based immunoaffinity with mass spectrometry to analytically va