ライフサイエンスコーパス: immunoblotting

1 pparent molecular mass (~40 kDa) observed by immunoblotting.

2 HLA-DM expression was analyzed by means of immunoblotting.

3 ancer cell lines by immunohistochemistry and immunoblotting.

4 d haptoglobin were also validated by western immunoblotting.

5 tive comparisons of selected key proteins by immunoblotting.

6 ns by qRT-PCR, and protein concentrations by immunoblotting.

7 e and polyacrylamide gel electrophoresis and immunoblotting.

8 olecules and to S. aureus and E. coli by IgE immunoblotting.

9 er tissues were analyzed by histology and by immunoblotting.

10 ppocampi and spinal cords were collected for immunoblotting.

11 the downstream targets of HDAC4 knockdown by immunoblotting.

12 ied by enzyme-linked immunosorbent assay and immunoblotting.

13 pha complexes in cells with blue native PAGE immunoblotting.

14 rology consisting of two-dimensional Western-immunoblotting.

15 ation as determined by mass spectrometry and immunoblotting.

16 despite no change in total TPBG detected by immunoblotting.

17 lected to assess MMP and RANKL production by immunoblotting.

18 and signaling pathways were determined using immunoblotting.

19 y available BACE1 inhibitor, was verified by immunoblotting.

20 Conversion of LC3 was further validated by immunoblotting.

21 ive or equivocal, is followed by second-tier immunoblotting.

22 cryptosporidiosis through immunostaining and immunoblotting.

23 were studied by immunohistochemistry and by immunoblotting.

24 s (SPTs), specific IgEs (sIgEs) and SDS-PAGE immunoblotting.

25 which is a convenient alternative method to immunoblotting.

26 tive real-time polymerase chain reaction and immunoblotting.

27 , 17d, and 25b inhibited pERK1/2 and pAkt by immunoblotting.

28 l populations by techniques such as ELISA or immunoblotting.

29 f CXCR3 and CCR7 by immunohistochemistry and immunoblotting.

30 acterized by competition studies, ELISAs and immunoblotting.

31 ionylated serpins A1 and A3 were assessed by immunoblotting.

32 molecular integrity was assessed by SDS-PAGE immunoblotting.

33 inoculation or tick bite were collected for immunoblotting against B. miyamotoi membrane-associated

34 Immunoblotting against the protein extracts revealed dif

35 Immunoblotting along with cryo-EM showed gingipain expre

36 clear extracts followed by mass spectrometry-immunoblotting analyses revealed that, upon TSEC treatme

37 RNA-Seq and immunoblotting analyses showed that MEOX1 knockdown decr

38 ay crystallography, protein engineering, and immunoblotting analyses, here we investigated the struct

39 ng co-immunoprecipitation followed by MS and immunoblotting analyses, we demonstrate the association

40 Using real-time RT-PCR and immunoblotting analyses, we measured mRNA expressions an

41 Immunoblotting analysis confirmed the S-acylation of fiv

42 eine-cysteine crosslinked samples, capillary immunoblotting analysis demonstrated that depletion of S

43 f ubiquitylation events in ask1 estimated by immunoblotting analysis in this work.

44 Our qRT-PCR and immunoblotting analysis revealed that reduced levels of

45 Immunoblotting analysis revealed that sirtinol significa

46 Immunoblotting analysis showed that Kif2a was gradually

47 Immunoblotting analysis using methyllysine-specific anti

48 These data are confirmed by immunoblotting analysis, which showed a significant redu

49 lenge to argan powder, skin prick tests, and immunoblotting analysis.

50 idual components of the ETC were assessed by immunoblotting and cellular complex IV activity was anal

51 Parallel native PAGE, immunoblotting and Complex I activity assays furthermore

52 Interestingly, by immunoblotting and confocal fluorescence microscopy, we

53 Immunoblotting and confocal microscopy results showed th

54 .1 cells (P < 0.05, n = 3), as determined by immunoblotting and densitometry.

55 ol mice and this finding was corroborated by immunoblotting and electrophoretic mobility shift assays

56 bovine gamma globulin (BGG) was assessed by immunoblotting and ELISA, respectively.

57 expression by RNA sequencing, and performed immunoblotting and ELISA.

58 Immunoblotting and enzyme-linked immunosorbent assay stu

59 Instead, using immunoblotting and enzyme-linked immunosorbent assays (E

60 phages was investigated by quantitative PCR, immunoblotting and flow cytometry.

61 activity were examined using real-time PCR, immunoblotting and flow cytometry.

62 Here, using subcellular fractionation, immunoblotting and fluorescence, siRNA-based gene knockd

63 Immunoblotting and histochemical analysis confirmed the

64 were collected at the end of each study for immunoblotting and histological studies.

65 d in the spinal cord, which was confirmed by immunoblotting and immunocytochemical labelling.

66 molecular, transmission electron microscopy, immunoblotting and immunofluorescence analyses, we studi

67 Here, using immunoblotting and immunofluorescence staining, qRT-PCR,

68 tative polymerase chain reaction followed by immunoblotting and immunofluorescence.

69 The GluN2D subunit was detectable by immunoblotting and immunohistochemistry in all subfields

70 rformed in transfection experiments by using immunoblotting and immunoprecipitation in STAT1-deficien

71 Our immunoblotting and immunostaining analyses revealed that

72 Using real-time RT-PCR, immunoblotting and immunostaining analyses, we measured

73 Immunoblotting and immunostaining of brain samples from

74 In addition, immunoblotting and immunostaining revealed an upregulati

75 We conducted immunoblotting and in vivo microdialysis procedures in M

76 we assessed NEMO linear ubiquitination using immunoblotting and investigated the formation of NEMO-co

77 ariance in a limited cohort was supported by immunoblotting and is consistent with mechanisms previou

78 nd an array of biochemical methods including immunoblotting and kinase assays, we show that sirtuin 2

79 Immunoblotting and kinetic and MS/MS experiments reveale

80 the Luminex Multiplex assay, ELISA, PCR, and immunoblotting and linked to the presence of EETs.

81 tch, respectively, and were then analyzed by immunoblotting and mass spectrometry for autophagy, apop

82 Using imaging, quantitative immunoblotting and mass spectrometry, we show that hundr

83 them using transmission electron microscopy, immunoblotting and mass spectrometry.

84 al cancer cell lines, which were analyzed by immunoblotting and proliferation and colony formation as

85 Through a series of immunoblotting and quantitative PCR (qPCR) experiments,

86 expression of TSHR in thymocytes by protein immunoblotting and quantitative PCR, and show that expre

87 Using Western immunoblotting and quantitative polymerase chain reactio

88 podocytes was confirmed in cultured cells by immunoblotting and quantitative real-time PCR and in mou

89 eme limiting dilution assays as well as with immunoblotting and quantitative real-time PCR for the ex

90 We confirmed these results by immunoblotting and selected protease and glycosidase act

91 Immunoblotting and selective reaction monitoring were us

92 Immunoblotting and sequence-translation analysis was per

93 We quantified protein changes with immunoblotting and t-SP by measuring dendritic spine mor

94 EGFR and RELA was validated by both qPCR and immunoblotting and they were both shown to be under dire

95 e impact of the mutation was investigated by immunoblotting and transcriptome sequencing.

96 By using immunoblotting and ultra-performance liquid chromatograp

97 two EIA strategies were followed by Western immunoblotting and when used in an MTTT, respectively.

98 allelic exchange, quantitative PCR analyses, immunoblotting, and (13)C-heme uptake experiments, we de

99 otein modeling, immunofluorescence staining, immunoblotting, and an enzymatic assay to evaluate the c

100 We also used immunoprecipitation assays, immunoblotting, and an in situ proximity ligation assay

101 n were assessed using reverse protein array, immunoblotting, and chromatin immunoprecipitation (ChIP)

102 Using chromogenic substrate assays, immunoblotting, and ELISA, we analyzed expression media,

103 t and feed varieties using quantitative PCR, immunoblotting, and enzyme-linked immunosorbent assay (E

104 sgenic technology, CRISPR-Cas9 gene editing, immunoblotting, and fluorescence resonance energy transf

105 s by using flow cytometry, confocal imaging, immunoblotting, and fluorimetric assays.

106 We show by immunofluorescence, immunoblotting, and glycosylation analysis that the vari

107 rected mutagenesis, Click O-GlcNAc labeling, immunoblotting, and immunofluorescence and EM imaging, w

108 kers by real-time polymerase chain reaction, immunoblotting, and immunofluorescence.

109 rse transcriptase-polymerase chain reaction, immunoblotting, and immunofluorescence.

110 MLECs were authenticated by CD31 immunoblotting, and immunofluorescent staining of establ

111 y cell cultures along with quantitative PCR, immunoblotting, and immunohistochemistry, we tested whet

112 ne knock-in technology and quantitative PCR, immunoblotting, and immunoprecipitation assays, we show

113 Using several mutant mouse strains, immunoblotting, and microcomputed tomography, we demonst

114 gel-based and shotgun proteomics, 1D and 2D immunoblotting, and quantitative ELISA were applied.

115 lyses of AKT-related genes using microarray, immunoblotting, and real-time quantitative PCR indicated

116 thological evaluation, immunohistochemistry, immunoblotting, and RNA sequencing.

117 positive or equivocal is reflexed to Western immunoblotting as the second tier.

118 in hippocampus and cortex were detected with immunoblotting assay.

119 unohistochemistry, biochemical, RT-qPCR, and immunoblotting assays revealed that Sema3d inhibits para

120 NAJC7 exceeded genome-wide significance, and immunoblotting assays showed depletion of DNAJC7 protein

121 MTT, qPCR and immunoblotting assays tested the effects of cottonseed e

122 Here, using immunofluorescence immunoblotting assays, co-immunoprecipitation, siRNA-med

123 with endocytosis, Lucifer Yellow-based, and immunoblotting assays, identified an elaborate signaling

124 s, fluorescence microscopy, and pulldown and immunoblotting assays, we show that alpha-dystrobrevin (

125 n addition, the signal-to-noise ratio of the immunoblotting by TDCS can be markedly increased.

126 By immunoblotting, CD36 but not cartilage intermediate laye

127 Using a combination of immunoblotting, co-immunoprecipitation, and myosin-bindi

128 Immunoblotting confirmation further supported this obser

129 Immunoblotting confirmed higher abundances of the select

130 In a set of sarcoma cell lines, immunoblotting confirmed nuclear localization of YAP1 an

131 Luciferase-expressing plasmid constructs and immunoblotting confirmed several predicted miRNA targets

132 Western immunoblotting confirmed that autophagy is not activated

133 sing a combination of patch clamp recording, immunoblotting, confocal imaging and structural modellin

134 itochondrial matrix volume is determined via immunoblotting, confocal microscopy of intact cells, and

135 Immunoblotting data agreed with mRNA changes.

136 Western immunoblotting demonstrated a 60% elevation of myocardia

137 Instead, immunoblotting demonstrated proteoforms of ADAM8 that la

138 Immunoblotting demonstrated that SLT11 (pCZ1) and SLT12

139 RNA sequencing and immunoblotting demonstrated that the risk allele locally

140 Circular dichroism, UV-Vis spectroscopy, and immunoblotting determined their capacity to (i) bind to

141 oxidation and cell proliferation analysed by immunoblotting did not show differences between BT and n

142 ion, and knockdown via mimics and anti-miRs, immunoblotting, dual luciferase reporter assay, in vivo

143 production, and cell death were evaluated by immunoblotting, ELISA, and cell death assays, respective

144 Here, using immunoblotting, ELISA, and surface plasmon resonance ana

145 ohistochemistry, real-time quantitative PCR, immunoblotting, ELISA, siRNA-mediated gene silencing, pl

146 Immunoblotting experiments confirmed that HP-bicarbonate

147 his nonapoptotic death pathway, we performed immunoblotting experiments in the presence and absence o

148 Immunoblotting findings of mitochondrial and synaptic pr

149 Here, using metabolic assays, immunoblotting, flow cytometry analyses, and siRNA-media

150 Using immunoblotting, flow cytometry, and LC-MS-based glycolip

151 ochondrial integrity by immunocytochemistry, immunoblotting, flow cytometry, and real-time PCR to qua

152 ed and tumors were collected and analyzed by immunoblotting for levels of RNF128, p53, and acetylated

153 lamide Gel Electrophoresis (BN-PAGE) and dot immunoblotting for quantifying various photosystem II (P

154 mRNA (RT-PCR) and protein (immunoblotting) for OAT1, OAT3, NaDC3, and MRP4 were det

155 s, a strong signal at ~70kDa was detected by immunoblotting, for which mass spectrometry revealed Dre

156 niques, including site-directed mutagenesis, immunoblotting, FRET, and proximity-ligation assays, we

157 Immunoblotting from fibroblasts and myoblasts of an affe

158 Parallel use of flow cytometry and immunoblotting further enabled us to estimate the densit

159 ms of TRAF2, along with immunoprecipitation, immunoblotting, gene expression, and immunofluorescence

160 In this study, we used mutational analysis, immunoblotting, HEK293 cells, and immunofluorescence mic

161 s panel was tested with two reference tests, immunoblotting (IB) and neutralization (Nt), and with 8

162 Using immunoblotting, imaging and mass spectrometry, we use ou

163 Immunoblotting, immunocytochemistry (ICC), and functiona

164 sed for studies including toxicant exposure, immunoblotting, immunofluorescence analysis, tight junct

165 NA complexes, but not free topo I or DNA, by immunoblotting, immunofluorescence or flow cytometry.

166 nvolved mitochondrial function; results from immunoblotting, immunofluorescence, and functional assay

167 In addition, immunoblotting, immunofluorescence, confocal imaging, an

168 including cell biology, RT-quantitative PCR, immunoblotting, immunofluorescence, flow cytometry, and

169 Immunoblotting, immunofluorescence, immunohistochemistry

170 atiometric calcium imaging with quantitative immunoblotting, immunofluorescent confocal microscopy, a

171 Sanger sequencing, immunoblotting, immunohistochemical testing, flow cytome

172 Immunoblotting, immunohistochemistry, and enzyme treatme

173 rial function and autophagy were assessed by immunoblotting, immunohistochemistry, and qPCR.

174 on and livers were collected and analyzed by immunoblotting, immunohistochemistry, histology, and rea

175 cells and transfected HEK293T cells through immunoblotting, immunohistochemistry, luciferase activit

176 ncluding efflux assays, immunoprecipitation, immunoblotting, immunohistochemistry, paracellular perme

177 Whole-exome sequencing, immunoblotting, immunophenotyping, and in vitro assays o

178 ned a wide array of approaches, ranging from immunoblotting, immunoprecipitation, mass spectrometry,

179 Immunoblotting, immunopurification, mass spectrometry an

180 We used electrophysiology studies, immunoblotting, immunostaining, and renal clearance to e

181 ish allergen parvalbumin was not detected by immunoblotting in 6/26 extracts.

182 ted repression of GMPS could be validated by immunoblotting in Sk-Hep1, HepG2, and HuH6 cells.

183 We validated these data by quantitative immunoblotting in striatal cell lines and human HD brain

184 Validations were by immunoblotting in systemic and intracoronary blood from

185 Using immunoblotting, in situ zymography, and immunofluorescen

186 igitoxin followed by immunoprecipitation and immunoblotting indicated that DARPP-32 has an important

187 Additionally, immunoblotting indicated that yeast coq11Delta mutants a

188 nce that a 2-EIA-based MTTT algorithm, where immunoblotting is replaced by the C6 EIA, performs as we

189 Immunoblotting is widely used for the detection of prote

190 n array of biochemical approaches, including immunoblotting, kinase assays, immunoprecipitation, and

191 We used structural modeling, immunoblotting, live cell imaging, and split green fluor

192 La cells, along with immunoprecipitation and immunoblotting, live-cell imaging, and protein-stability

193 Immunoblotting, mass spectrometry, and ultrafast spectro

194 Analysis by immunoblotting, metabolic labeling, and mass spectrometr

195 We present here a new immunoblotting method, which is characterized by excepti

196 Using biochemical and immunoblotting methods, mitochondrial function and phosp

197 nockout mice, subcellular fractionation, and immunoblotting methods, we addressed the relationship of

198 sing confocal immunofluorescence microscopy, immunoblotting, myeloperoxidase-DNA complex ELISA, and f

199 Herein, we used a combination of immunoblotting, neuropharmacological and transgenic proc

200 tive RT-PCR; protein content was measured by immunoblotting; NOS activity was evaluated with high-per

201 d caspase-3, and extravascular albumin), and immunoblotting (nuclear factor-kappaB, hypoxia-inducible

202 Immunoblotting of FECD ex vivo specimens revealed an acc

203 Comprehensive quantitative immunoblotting of protein extracts from human embryonic

204 Alpha-gal epitopes were detected by immunoblotting on antivenoms.

205 for choline/methyl metabolite measurements, immunoblotting or gene expression of relevant enzymes.

206 f antiapoptotic BCL2 family members based on immunoblotting or RNA transcript levels correlated poorl

207 as analyzed by using phospho flow cytometry, immunoblotting, or co-immunoprecipitation in CD19-defici

208 xpression of signaling molecules by means of immunoblotting, PGD2 and macrophage inflammatory protein

209 ration markers to be assayed in combined IEF-immunoblotting procedures; the latter ones showing optim

210 tified by transcriptome sequencing, qRT-PCR, immunoblotting, protein interaction studies, knockdown a

211 Here, using murine ventricular myocytes, immunoblotting, proximity ligation as-says, and nitric o

212 uses after drug treatment were determined by immunoblotting, proximity ligation, replicon DNA replica

213 By immunoblotting pyrin after infection, we observed that w

214 In this work, using ChIP-sequencing, immunoblotting, quantitative PCR, and computational anal

215 mug/mL), or both (CSE+EtOH), and analyzed by immunoblotting, quantitative reverse-transcription polym

216 proaches, along with immunoprecipitation and immunoblotting, quantitative RT-PCR, and ELISAs, we foun

217 situ hybridization, primary cell isolation, immunoblotting, quantitative RT-PCR, and liquid chromato

218 Here, using isolated MV, immunoblotting, quantitative RT-PCR, FACS analysis, and

219 r cell lines, siRNA-mediated gene silencing, immunoblotting, quantitative RT-PCR, promoter-reporter a

220 opment and analyzed by immunohistochemistry, immunoblotting, real-time polymerase chain reaction, and

221 myocytes, lucigenin chemiluminescence assay, immunoblotting, real-time polymerase chain reaction, imm

222 with knockdown of LKB1 or other proteins by immunoblotting, real-time quantitative polymerase chain

223 ll lines via indirect immunofluorescence and immunoblotting, respectively.

224 h quantitative polymerase chain reaction and immunoblotting, respectively.

225 ttern of DMT1 regulation was corroborated by immunoblotting results in diabetic mice showing that BBM

226 Immunoblotting results revealed that the astrocytes prop

227 Flow cytometric and immunoblotting results suggest that CurDD can induce Hep

228 Immunoblotting revealed a decrease of AQP5 protein abund

229 Immunoblotting revealed downregulation of the medullary

230 In the XLH cultures, immunoblotting revealed more abundant osteopontin (OPN),

231 Immunoblotting revealed rab17 immunoreactive species at

232 e-negative breast cancer cell viability, and immunoblotting revealed that impaired growth is due to p

233 icroarray analysis, quantitative RT-PCR, and immunoblotting revealed that IRF2 regulated the basal le

234 Mass spectrometry and immunoblotting revealed that the costimulatory factor in

235 Immunoblotting revealed that this was associated with ac

236 By immunoblotting, RIP9, OTP86, OZ1 and ORRM1 were shown to

237 using HEK 293T cells and immunofluorescence, immunoblotting, RNAi, subcellular fractionation, co-immu

238 Using immunoprecipitation and immunoblotting, RT-quantitative PCR, and ChIP assays, we

239 In genipin-treated TM cells, Western immunoblotting showed a reduction of active MMP2 and MMP

240 fate poly acrylamide gel electrophoresis and immunoblotting showed acid-soluble collagen fraction fro

241 sed on the surface of CLL cells, and Western immunoblotting showed an inverse correlation between Wnt

242 Transcriptome analyses, real-time PCR, and immunoblotting showed consistent reductions in the expre

243 Immunoblotting showed elevated levels of gamma-H2AX, Bcl

244 Western immunoblotting showed increases of the iron storage prot

245 N-beta than was the wild-type S. aureus, and immunoblotting showed that IFN-beta interacts with the b

246 Immunoblotting showed that procyanidin C1 and chlorogeni

247 Immunoblotting showed that the superior/middle lateral R

248 Here, with the help of an immunoblotting strategy and Ime4-GFP protein localizatio

249 Targeted LC-MS/MS and immunoblotting studies further confirmed the down regula

250 Moreover, immunoblotting studies of selectively bred and transgeni

251 -activated protein kinase, and Src, shown by immunoblotting studies.

252 Immunoblotting suggested that SerpinB2 (cross-linked int

253 sphorylation was detected by two-dimensional immunoblotting, suggesting that loss of SMA-5 function l

254 Here, by applying immunoblotting, targeted phosphoproteomics and metabolit

255 Western immunoblotting techniques detected higher CML levels in

256 chromatography, and nondenaturing/denaturing immunoblotting techniques.

257 reeze-dried in order to perform SDS-PAGE and immunoblotting tests.

258 monstrate by immunofluorescence staining and immunoblotting the presence of galectin-8 within the mit

259 In the immunoblotting, the patient's IgE reacted with 18 and 30

260 n the basolateral medium was investigated by immunoblotting, thin-layer chromatography with immunosta

261 ophysiology, renal clearance techniques, and immunoblotting to examine effects of Kir4.1/Kir5.1 in th

262 icroscopy, immunohistochemistry, and Western immunoblotting to examine the morphology and microstruct

263 We applied quantitative immunoblotting to generate profiles of transporters, cha

264 ss-linking followed by mass spectrometry and immunoblotting to isolate and characterize proteins comp

265 human serum IgG4 were identified by means of immunoblotting to screen for potential bacterial allerge

266 Using immunoblotting to specifically examine the expression le

267 used electrophysiology, renal clearance, and immunoblotting to study Kir4.1 in the DCT and NCC in Kir

268 We employed affinity purification and immunoblotting to validate the interaction between STAT5

269 pression in human cells and when assessed by immunoblotting under reducing and denaturing conditions,

270 Immunoblotting using an Ser-235 phosphorylation-specific

271 and heated protein extracts was evaluated by immunoblotting using five allergen-specific antibodies a

272 tin modifications were determined by Western immunoblotting using specific antibodies.

273 IgE-immunoblotting was also conducted using sera from childr

274 Immunoblotting was employed on tissue from subregions of

275 Because immunoblotting was found to be inefficient for most of t

276 IgE immunoblotting was performed with pooled sera from food-

277 rm of TBCD and using non-denaturing gels and immunoblotting, we analyzed lysates from a number of mou

278 as9 gene-editing, MARCH6 overexpression, and immunoblotting, we found here that cholesterol stabilize

279 In addition, through immunoblotting, we found that AHAs overexpressed the NMD

280 ch-clamp recordings, confocal microscopy and immunoblotting, we found that both the GABRB3 (N328D) an

281 confirmation by pulldown assays followed by immunoblotting, we identified P-selectin and multimerin-

282 Using double-thymidine synchronization and immunoblotting, we observed that MELK inhibition delays

283 Using fluorescence microscopy and immunoblotting, we show here that the endogenously produ

284 tion mutants, quantitative PCR analyses, and immunoblotting, we show that the activation of the sigma

285 Analytic isoelectric focusing and immunoblotting were conducted on cellular extracts of B.

286 lectron microscopy, histologic analyses, and immunoblotting were used to determine the effects of ATP

287 Immunofluorescence and immunoblotting were utilized to detect surface-localized

288 e binding to beta-tubulin is demonstrated by immunoblotting, which allows for determination of the re

289 PO7, coupled with phosphopeptide mapping and immunoblotting with a phosphoserine-specific PKC substra

290 ifying 16 allergens based on two-dimensional immunoblotting with A. terreus susceptible patient sera.

291 e detection of His-tagged proteins relies on immunoblotting with anti-His antibodies.

292 ayed decreased phospho-signal intensities in immunoblotting with anti-phosphoserine/phosphothreonine

293 rometry, followed by SDS-PAGE and subsequent immunoblotting with antibodies detecting 4 fish allergen

294 Using immunoblotting with CLCA1-specific antibodies and recomb

295 tial scanning fluorimetry, CD, SDS-PAGE, and immunoblotting with conformation-dependent and -independ

296 Rca-beta wild-type control, as evidenced by immunoblotting with custom antibodies and quantitative m

297 caspase-9 (CC-9) expression were assayed by immunoblotting with or without SIRT3 enforced expression

298 -polyacrylamide gel electrophoresis SDS-PAGE-immunoblotting with patient sera and rabbit serum anti-P

299 IgE-immunoblotting with peach leaf extract revealed in six p

300 st increased family, as confirmed by western immunoblotting, with a stronger reactivity in SAU mastit