ライフサイエンスコーパス: initial velocity

1 t in the centre, and it is influenced by the initial velocity.

2 ains both substrate and pH dependency of the initial velocity.

3 ranes (Michaelis constant, 20-40 nm; maximum initial velocity, 26-29 nmol g-1 fresh weight h-1), wher

4 The initial velocity (after 1-s microwave heating) of the bi

5 absence of the second Mg(2+) as evidenced by initial velocity analysis and substrate analogue and pro

6 m, random kinetic mechanism, as evidenced by initial velocity analysis indicating sequential addition

7 11 +/- 0.06 obtained by direct comparison of initial velocities and 1.2 obtained by single-wavelength

8 ere was a sigmoidal relationship between the initial velocity and bulk substrate concentration.

9 At low pH (5.7) the initial velocity and dead-end inhibition patterns are co

10 Initial velocity and dead-end inhibition studies support

11 Initial velocity and dead-end inhibitor studies with the

12 Initial velocity and inhibition kinetic studies support

13 trate binding to the enzyme, as evidenced by initial velocity and inhibition mechanism studies with n

14 Initial velocity and inhibition studies show that the st

15 Initial velocity and inhibition studies support a random

16 method for measurement of calcium influx at initial velocity and obviate concern for "unstirred laye

17 The results of initial velocity and product and dead-end inhibition exp

18 Initial velocity and product inhibition kinetic data are

19 Steady-state initial velocity and product inhibition kinetic studies

20 The steady-state initial velocity and product inhibition patterns are con

21 Initial velocity and product inhibition studies indicate

22 heterologously expressed and purified GmHSD, initial velocity and product inhibition studies support

23 Kinetic characterization, utilizing initial velocity and product inhibition studies, found t

24 licated profiles in the relationship between initial velocity and substrate concentration, making it

25 dispersion can be more important than large initial velocity and turbulent transport with dilute sus

26 NADPH is only 30% (initial velocities) as effective as NADH.

27 Under initial velocity assay condition of low product formatio

28 phorylation, were followed by monitoring the initial velocity at which the enzyme catalyzes the phosp

29 The IFE distortion of apparent initial velocities can be corrected without doing fluoro

30 Initial velocity data indicate the MgHIc complex is the

31 Global fitting analysis of initial velocity data indicates that a random terreactan

32 Comparison of the initial velocity data using AdoMet versus [2H3-methyl]Ad

33 Initial velocity data with DNA and S-adenosylmethionine

34 Initial velocity data with peptide and S-adenosyl-L-meth

35 H effects demonstrated in progress-curve and initial-velocity data sets from rat, human, Escherichia

36 g product and substrate inhibition analyses, initial velocity dependence between substrates, and isot

37 Initial velocity determinations were conducted with huma

38 t of the desorbed ions, thereby reducing the initial velocity distribution and improving resolution.

39 cs we must include a sub-cycle change of the initial velocity distribution of the electron and its ex

40 Initial velocity experiments demonstrate that dimerizati

41 Equilibrium binding and initial velocity experiments revealed a less than 2-fold

42 From initial velocity experiments, catalytic constants for su

43 From initial velocity experiments, the Michaelis constant for

44 Kia value for this substrate determined from initial velocity experiments.

45 3D(pol) to obtain a reliable estimate of the initial velocity in as little as 4 min.

46 retained over a broad pH range, and apparent initial velocities indicate that maximal activities are

47 than those values determined from reciprocal initial velocity-initial substrate concentration plots f

48 inner filtering effect (IFE) alters apparent initial velocities, K(m), and k(cat).

49 Initial velocity kinetic studies indicated that Disperse

50 soybean root nodules has been determined by initial velocity kinetic studies monitoring oxygen uptak

51 for the synthetase from Escherichia coli by initial velocity kinetics and patterns of linear inhibit

52 ons per substrate molecule with very similar initial velocity kinetics and pH dependencies.

53 The initial velocity kinetics of these activities exhibited

54 On the basis of initial velocity kinetics, Tl+ enhances catalysis by 20%

55 spectroscopy, fluorescence spectroscopy, and initial velocity kinetics.

56 Initial velocity line patterns were also determined when

57 nstants for DXP synthase calculated from the initial velocities measured at varying concentrations of

58 Initial velocity measurements and inhibitor studies were

59 Initial velocity measurements have been used to measure

60 Initial velocity measurements indicated that the rate of

61 Yet, initial velocity measurements over the same temperature

62 ical approach using reaction timecourses and initial velocity measurements to determine the actual co

63 ERK2 was examined, with excess magnesium, by initial velocity measurements, both in the absence and p

64 ard and reverse reactions were determined by initial velocity measurements.

65 re substrates, as demonstrated by comparable initial velocity measurements.

66 o iminoribitol alone (28 pM vs 2.9 mM), from initial velocity measurements.

67 The initial-velocity method is simple and useful for general

68 oints of measured product concentration (the initial-velocity method), and (2) by fitting all the dat

69 tic mechanism of AtGS was investigated using initial velocity methods and product inhibition studies.

70 Initial velocity methods were used to probe the kinetic

71 Initial velocities of [3H]mannose uptake were two- to th

72 Double reciprocal analysis of initial velocities of AcpS at various concentrations of

73 S) for the quantitative determination of the initial velocities of four heparin-modifying enzymes.

74 onventional steady-state kinetics as well as initial velocities of mixed substrate cleavages under th

75 Initial velocities of PC activation at different concent

76 of activation were assessed by measuring the initial velocities of the phosphorylation of the tyrosin

77 As predicted by the model, the initial velocity of 12-AS arrival at the acceptor membra

78 mbrane vesicles, translocase activity had an initial velocity of 3.3 nmol phosphatidylserine (PS) tra

79 The traditional method follows the initial velocity of [32P]PPi incorporation into ATP by c

80 that propagated along the arteriole with an initial velocity of approximately 116 microm/s (n=28) an

81 of unbound analyte to be determined from the initial velocity of binding.

82 distinct functional consequences: increased initial velocity of fibrin clot formation, altered fibri

83 reactions of selenoxide 8 with H(2)O(2).The initial velocity of oxidation of PhSH by H(2)O(2) with s

84 ral correlate of these neural responses: the initial velocity of pursuit eye movements deviates in a

85 EhUba1 exhibits a greater maximal initial velocity of pyrophosphate:ATP exchange than its

86 We show that while the initial velocity of the assembly depends on Tim9 concent

87 g phosphatidylcholine/cholesterol, 1:1), the initial velocity of the mutant is reduced 3000-fold.

88 product scatters forward with respect to the initial velocity of the O atom, and the H atom scatters

89 d under the same conditions by following the initial velocity of the phosphorylation of peptides cont

90 xamination of transporter kinetics utilizing initial velocity of uptake revealed that METH treatment

91 The initial velocity of VPA uptake varied in proportion with

92 DYNAFIT was developed for fitting either the initial velocities or the time course of enzyme reaction

93 The initial velocity pattern in which the ratio between the

94 A parallel initial velocity pattern that displays competitive subst

95 Intersecting initial velocity patterns for both glucose and ATP indic

96 Data from initial velocity patterns in the presence and absence of

97 mechanism for JNK3alpha1 was determined via initial velocity patterns in the presence and absence of

98 and fixed concentrations of oxamate revealed initial velocity patterns inconsistent with a simple pin

99 Initial velocity patterns indicate that both domains fol

100 Analysis of the initial velocity patterns indicated a sequential mechani

101 Parallel initial velocity patterns indicated that both substrates

102 The initial velocity patterns indicated that the kinetic mec

103 Bi-substrate kinetic analysis gave initial velocity patterns indicating a sequential mechan

104 Analysis of initial velocity patterns suggests a random sequential k

105 Initial velocity patterns were consistent with a sequent

106 ocal plots with UTP produced parallel lines, initial velocity plots with other phosphate donors and p

107 nd PPi is the last product released based on initial velocity, product and dead-end inhibition studie

108 substrate were used to perform steady-state initial velocity, product inhibition, and dead end inhib

109 Initial velocity, product inhibition, and dead-end analo

110 Initial velocity, product inhibition, and dead-end inhib

111 Using a combination of initial velocity, product inhibition, and dead-end inhib

112 lucokinase reactions were investigated using initial velocity, product inhibition, and dead-end inhib

113 Initial velocity, product inhibition, and dead-end inhib

114 Initial velocity, product inhibition, and deuterium kine

115 Initial velocity, product inhibition, dead-end inhibitio

116 Thus, initial velocity, product, and dead-end inhibition data

117 Finally, the results of initial velocity, product, and dead-end inhibition studi

118 The results of initial velocity, product, and dead-end inhibition studi

119 Initial velocity, product, and dead-end inhibition studi

120 Initial velocity profiles were consistent with an ordere

121 The initial velocity results were equally consistent with in

122 o the starting thiol ester was detected with initial velocity solvent isotope exchange experiments.

123 ed friction has complex evolution, featuring initial velocity strengthening followed by substantial v

124 Initial velocity studies confirm the change in mechanism

125 Initial velocity studies demonstrate that APH(2'')-Ib op

126 bserved in both isotope exchange studies and initial velocity studies has not yet been identified.

127 Isotope partitioning and initial velocity studies have been used to study the kin

128 carboxylation of isocitrate, on the basis of initial velocity studies in the absence and presence of

129 accharomyces cerevisiae was determined using initial velocity studies in the absence and presence of

130 Initial velocity studies indicate that MAMS follows an o

131 analysis of PAP was conducted which included initial velocity studies of the forward and reverse reac

132 Initial velocity studies reveal that Rv2747 proceeds thr

133 Initial velocity studies revealed that all AKT isozymes

134 Initial velocity studies show that EntE proceeds via a b

135 Initial velocity studies show that IIAGlc is an uncompet

136 Initial velocity studies show the labeling does not alte

137 dD33 prefers long chain saturated lipids and initial velocity studies showed that FadD33 proceeds via

138 Initial velocity studies support an ordered kinetic mech

139 Initial velocity studies were consistent with a sequenti

140 ctivator, was present at a saturating level, initial velocity studies were consistent with a Theorell

141 Initial velocity studies with L-glutamate showed that ev

142 Site-directed mutagenesis, initial velocity studies, pH-rate studies, and substrate

143 Initial velocity studies, product inhibition, and dead-e

144 On the basis of initial velocity studies, the selectivity of the activat

145 An initial velocity study indicated an ordered bi-bi cataly

146 Initial velocity techniques were used to define the stea

147 40 microM are biphasic and characterized by initial velocities that decay by a first-order process t

148 isulfide formation in a redox buffer with an initial velocity that is 30-fold faster than PDI.

149 Comparing the simulated activity (ratio of initial velocity to the enzyme concentration) under phys

150 iaryltellurides 3 and 4 as determined by the initial velocities, v(0), for the rate of appearance of

151 Double-reciprocal plots of initial velocities versus the varying concentrations of

152 Plots of initial velocity versus free magnesium concentration are

153 their values determined from the reciprocal initial velocity versus initial substrate concentration

154 Plots of initial velocity versus the concentration of the varied

155 A set of initial velocities was analyzed to see if a tight-bindin

156 The temperature dependence of initial velocities was used to estimate activation param

157 Although the measured initial velocity was higher when either fragment 2 or fr

158 the kinase domain of v-fps (GST-kin) and the initial velocities were determined by a coupled enzyme a

159 ved that the extent of hybridization and the initial velocities were directly dependent on the length

160 However, the observed initial velocities were too low to accurately determine