ライフサイエンスコーパス: insertion site

1 lecule with 50 bp of homology specifying its insertion site.

2 infection and colonization according to the insertion site.

3 atheters infection according to the catheter insertion site.

4 disruption was observed at the iStent inject insertion site.

5 eletions and/or structural variations at the insertion site.

6 ce, 23-25 nucleotides upstream of the intron insertion site.

7 position immediately adjacent to the intron insertion site.

8 ) and adjacent chromosomal DNA at the SCCmec insertion site.

9 FP reporter was inserted 5 kb from the bw(D) insertion site.

10 on of the hydrophobic core of Bn near the Ub insertion site.

11 pears to be a base preference (TTCT) for the insertion site.

12 ns for GC content and nucleotide bias at the insertion site.

13 of a sponge species, which also has the same insertion site.

14 ar magnetic resonance at the right ventricle insertion site.

15 other pathway terminating at the N terminus insertion site.

16 am-negative bacilli was high for the femoral insertion site.

17 characterized an unprecedented number of hsr insertion sites.

18 q by in-depth characterization of individual insertion sites.

19 exogenous sequences that sometimes surround insertion sites.

20 cause it contributes to the selection of new insertion sites.

21 could be distinguished due to differences in insertion sites.

22 occlusion onto the skin surrounding catheter insertion sites.

23 keep track of sequences, mappings and common insertion sites.

24 irs and higher orders of co-occurring common insertion sites.

25 ted host carries between 500 and 5000 unique insertion sites.

26 monin mechanically reinforces tip link upper insertion sites.

27 c regions that coincided with preferential P-insertion sites.

28 er the chromodomain contributed to selecting insertion sites.

29 tional sequencing to characterize transposon insertion sites.

30 and robust system for identifying transposon insertion sites.

31 s, and multiple strategies exist for mapping insertion sites.

32 asked region near the microdialysis catheter insertion sites.

33 The friend leukemia insertion site 1 (Fli-1) transcription factor, an Ets fa

34 or vascular complications at the 18-F sheath insertion site (2.1% vs. 11.3%; p = 0.003), and of all t

35 retroviral insertion at the ecotropic viral insertion site 32 locus leads to increased expression of

36 selected mutants and found that 33 out of 44 insertion sites (75%) could be confirmed by PCR, and 17

37 a uniform distribution of new peptidoglycan insertion sites, a necessary condition to maintain rod s

38 be used to determine gene involvement at the insertion site after FISH has identified the presence of

39 provides a graphical overview of the mapped insertion sites against a karyotype.

40 f echocardiographic scar at papillary muscle insertion sites (all P<0.05) and, when combined, were ad

41 e the diversity of genomic islands and their insertion sites among Gram-negative bacteria and discuss

42 differences in the methylation status of the insertion sites among MITE families.

43 stion-based approaches for ligation-mediated insertion site amplification.

44 Insertion site analyses of recently reported trials on a

45 Insertion-site analyses revealed the multipotent nature

46 Insertion site analysis demonstrated that more than 80%

47 Vector insertion site analysis of granulocytes demonstrated sus

48 Insertion site analysis of the library repeatedly identi

49 Vector insertion-site analysis was performed in seven infants a

50 e timing of methylation is influenced by the insertion site and by additional genetic information.

51 ust and efficient means to identify both the insertion site and content of transgenes through deep se

52 he sole variables were transgene chromosomal insertion site and copy number.

53 lineage demonstrated a preferred chromosomal insertion site and different complements of non-LEE enco

54 pport the preferential use of the subclavian insertion site and enhanced efforts to reduce dressing d

55 is approximately 4.7 kb upstream of the Mu1 insertion site and may be proximal to an adjacent repeat

56 Identification of the activation tag insertion site and microarray analysis revealed that ATH

57 n evolutionarily conserved fragment near the insertion site and observed enhancer activity of this el

58 QRS expression dependent on the ventricular insertion site and often coexisted with other tachycardi

59 Linkage between the insertion site and phenotype was confirmed, and we show

60 cing (INSeq) is a method for determining the insertion site and relative abundance of large numbers o

61 fered in several aspects, such as the intron insertion site and sequence length.

62 he tla2 phenotype, was cloned by mapping the insertion site and upon complementation with each of the

63 Varying the HPIV3 antigen insertion site and vector dose allowed fine-tuning of th

64 We detected 225,936 genomic Mu insertion sites and 41,086 high quality germinal Mu inse

65 s mapped to millions of potential transposon insertion sites and a large portion of insertion sites h

66 Determination of insertion sites and adjacent viral sequences identify th

67 e, we describe a method to recover transgene insertion sites and assess structural rearrangements of

68 lengths observed in each of a collection of insertion sites and is maximized with a hybrid expectati

69 This allowed us to identify the insertion sites and phenotypes of negatively selected mu

70 We present the full genomic sequences, insertion sites and phylogenetic analysis of 28 prophage

71 by cryogenic storage, (2) identify mutagenic insertion sites and physical coordinates in these collec

72 tant mice also had improper ureteral bladder insertion sites and shortened intravesicular tunnel leng

73 m distances were computed between the tendon insertion sites and the glenoid, acromion, and coracoid

74 m that detects both new transposable element insertion sites and their methylation states from a sing

75 asis genes were identified by sequencing the insertion sites and then mapping these sequences back to

76 ning, collection method, needle size, needle insertion site, and cerebrospinal fluid (CSF) volume col

77 nation of the intercondylar roof, ACL-tibial insertion site, and PCL angle and horizontal component-t

78 m a combination of host factor occupancy and insertion site architecture, and that this results in th

79 ndicating that the transgene promoter and/or insertion site are critical for sustained expression.

80 Insertion sites are compared among individuals of suitab

81 In the analysis of these screens, transposon insertion sites are typically identified by targeted DNA

82 Thirty-seven per cent (9754) of these insertion sites are within genes (including untranslated

83 We identified three resulting insertion sites (arrayed binding sites), the longest of

84 insert into sites other than its known TTAA insertion site at a low frequency (2%).

85 increase the toughness of the tendon-to-bone insertion site at the expense of its strength.

86 enome resequencing approaches, we identified insertion sites at four alleles of the LATERAL LEAFLET S

87 s from lymphomas/leukemias identified common insertion sites at known and candidate novel cancer gene

88 related dsRNA viruses detected preferential insertion sites at which the complexity of the conserved

89 d primary wound repair due to the leakage in insertion sites before the PPV, however remaining 20 cas

90 Therefore, focusing on the insertion site between the anterior cruciate ligament (A

91 Insertion site bias, or lack thereof, has not been demon

92 mation, the template base has moved into the insertion site but misaligns an incorrect nucleotide rel

93 mal translocations associated with the T-DNA insertion site, but the prevalence of these rearrangemen

94 t of a conserved active site tyrosine in the insertion site by the template base is accommodated by a

95 ndonuclease I-AniI and their putative target insertion sites by BLAST searches followed by examinatio

96 pread in two dimensions: horizontally across insertion sites by non-allelic gene conversion, and vert

97 x that identifies integrative MGEs and their insertion sites by using short-read sequencing data.

98 ion in vivo, by mating polB intein-positive [insertion site c in the gene encoding DNA polymerase B (

99 Insertion sites can then be navigated in Ensembl in the

100 and truncated versions of the element in new insertion sites cause stable mutations.

101 nsertions is unclear without high-resolution insertion site characterization, the potential for an ot

102 enables broader intein utility by increasing insertion site choices.

103 Common insertion site (CIS) analysis of 119 primary tumors and

104 Common insertion site (CIS) analysis of 269 neurofibromas and 1

105 eir genomic location is proximal to a common insertion site (CIS) defined by high rates of transposon

106 Analysis of transposon common insertion sites (CIS) identified the Apc locus as a majo

107 ransposons have been used to identify common insertion sites (CISs) associated with tumor formation.

108 nd the genomic location of transposon common insertion sites (CISs) from all currently published tran

109 By analyzing common insertion sites (CISs) isolated from 446 tumors, we iden

110 nts within the genome and 2) identify Common Insertion Sites (CISs) within the genome are not practic

111 ructure, the substrate binds to the active ('insertion') site closed through refolding of the trigger

112 ence and distribution, and (iii) to evaluate insertion site-dependent changes in mechanical propertie

113 nately, the great increase in the numbers of insertion sites detected comes with the cost of not know

114 EpiTEome outperforms other split-read insertion site detection programs, even while functionin

115 etermine whether genes located at transposon insertion sites directly caused medulloblastomas to diss

116 Insertion site dressings are a major mean to reduce cath

117 or some H chain genes could be caused by (i) insertion site effects or (ii) deletion of enhancer elem

118 icient evidence to recommend one CVC type or insertion site; femoral catheterization should be avoide

119 ite following transposon excision and at the insertion site following transposon integration.

120 Using this photoprobe, we demonstrated the insertion site for [ (125)I]IACoc to be Asp188, which re

121 and subsequent DNA bending primes a selected insertion site for efficient transposition.

122 Since the coronoid process provides an insertion site for the temporalis attachment, we examine

123 specificity data and defining new potential insertion sites for any target.

124 Comparison of insertion sites for IS492 and the highly related ISPtu2

125 the first large-scale analysis of transgene insertion sites from 40 highly used transgenic mouse lin

126 equencing technology and is able to identify insertion sites from both the 5' and 3' ends of the tran

127 loped IM-Fusion, an approach that identifies insertion sites from gene-transposon fusions in standard

128 ), that facilitates sequencing of transposon insertion sites from single tumor cells in a SB mouse mo

129 Sequence analysis of transposon insertion sites from tumors and immortalized cells ident

130 The common insertion site genes identified in the mutagenesis scree

131 Both of these insertion sites have previously been mapped to the "clam

132 poson insertion sites and a large portion of insertion sites have zero mapped reads.

133 Analysis of transposon common insertion sites identified 7 genes activated by transpos

134 Isolation of the transposon insertion sites identified genes known to be associated

135 Analysis of transposon insertion sites identified novel candidate genes, includ

136 Cloning DNA flanking the insertion site identifies the locus precisely.

137 a specific double-strand cut near the intron insertion site (IIS), its DNA recognition site spans the

138 moral site was the most frequently colonized insertion site in all types of catheters.

139 ogy such as fever, carefully inspect the PVC insertion site in bacteremic or fungemic patients, and r

140 sposons form genomic islands at a programmed insertion site in bacterial chromosomes, attTn7.

141 Selection of central venous catheter insertion site in ICU patients could help reduce cathete

142 point on the nephric duct (ND) to its final insertion site in the cloaca (the primitive bladder and

143 Using PCR, we identified a transposon insertion site in the first intron of Nmnat2 (Nicotinami

144 Jak2 was identified as a common transposon insertion site in TLS-ERG-induced disease, strongly vali

145 VL gene sequences, and the chromosomal phage insertion sites in 114 isolates comprising nine clones o

146 mutants with the desired phenotypes, and the insertion sites in 18 of the strains were mapped.

147 s, PVL gene sequences, and chromosomal phage insertion sites in 52 S. aureus CC30 PVL-harboring isola

148 After sequencing transposon insertion sites in 9,250 random mutants, we assembled a

149 e splice finding and elucidation of multiple insertion sites in a random insertional mutagenesis libr

150 es an efficient k-mer-based method to detect insertion sites in a reference genome, and subsequently

151 Pyrosequencing data showed 266 insertion sites in addition to the 325 sites expected fr

152 Sequence analysis of insertion sites in both R. rickettsii and R. prowazekii

153 we used a targeted method to sequence LINE-1 insertion sites in matched PDAC and normal samples.

154 Assessment of insertion sites in peripheral blood from patients in the

155 s in these collections, and (3) validate the insertion sites in pools of mutants by obtaining >500 bp

156 rately corresponded to LINE; retrotransposon insertion sites in ribosomal DNA (p<0.01).

157 us mapping of tens of thousands of mutagenic insertion sites in the eukaryotic unicellular green alga

158 To identify the location of insertion sites in the genome linker based polymerase ch

159 The insertion sites in the genome were pre-N (F1), N-P (F2),

160 Characterization of transposon insertion sites in the SB-induced tumors identified 45 r

161 Analysis of the mPing insertion sites in the soybean genome revealed that feat

162 This increased the number of unique insertion sites in the T-DNA collection by 21 078, bring

163 hods were established to identify transposon insertion sites in these lesions, and analysis of transp

164 were conspicuous among the common transposon insertion sites in TLS-ERG-driven leukemia, suggesting t

165 ic duplications were observed at or near the insertion sites in two patients, further confounding the

166 rvo-controlled actuation of syringeal muscle insertions sites in vitro and focus on two muscles contr

167 ed candidate prostate cancer genes at common insertion sites, including Pde4d.

168 identify retroviral insertions at 19 common insertion sites, including Zeb2, Nf1, Mn1, Evi1, Ift57,

169 However, with this method, the insertion site, integrity, and copy number of the transg

170 Insertion sites interrupting genes that are likely criti

171 We also have determined the optimal insertion site into the NDV genome by generating recombi

172 DNA superexon, spanning exon 2-27, with a 5' insertion site into the rat gene just beyond intron 1.

173 This approach allows for the clustering of insertion sites into distinct regions of essentiality ac

174 we introduced plasmids containing candidate insertion sites into S. pombe and mapped the positions o

175 Fgfr2(ST-/-) mice exhibit improper ureteral insertion sites into the bladder, consistent with the ur

176 The transgene insertion site is associated with a 12 kb deletion, 1.2

177 f target DNA engagement until an appropriate insertion site is identified.

178 Under the above-stated conditions, insertion site is likely the most contributory variable

179 tion assays, finding that for G1 and G2, the insertion site is recognized through defined mechanisms,

180 c and predictive impact of allelic ratio and insertion site (IS) of internal tandem duplications (ITD

181 cus and paired lines in the same chromosomal insertion site lacking the 3' enhancers.

182 tissues suggest that the DNA region near the insertion site likely interacts with Foxl2 TSS.

183 the utility of the method by identifying Mu insertion sites linked to seed-lethal mutations with a p

184 We identified numerous common insertion sites located near protein-coding genes and co

185 Insertion site mapping of cells that survived long-term

186 Two common retrovirus insertion sites near c-myb and Sox4 genes were identifie

187 ient population, number of catheters at each insertion site, number of catheter-related bloodstream i

188 eading frames were common, although based on insertion site-occupancy frequency data it appeared that

189 ect to copy number, nucleotide diversity and insertion site-occupancy frequency.

190 Common transposon insertion sites occur among haplotypes from different Ze

191 cer to resolve the structure and chromosomal insertion site of a composite antibiotic resistance isla

192 ied in 14 of the mutants on the basis of the insertion site of a transposable element.

193 e, ECM accurately identified the ventricular insertion site of an accessory atrioventricular connecti

194 Pan proviral insertion site of Moloney murine leukemia (PIM) 1, 2, an

195 The high expression of proviral insertion site of Moloney murine leukemia virus kinases

196 , a systematic study to evaluate the optimal insertion site of the foreign antigens into NDV that res

197 The transposon insertion site of this mutant strain was within Sca2, a

198 nd pathogenic mechanisms of mycoplasmas, the insertion site of transposon Tn4001T was determined for

199 The insertion sites of 17 copies of the G.st.I1 intron from

200 ECGI can noninvasively localize ventricular insertion sites of accessory pathways to guide ablation

201 The insertion sites of femoral catheters were involved in al

202 omparison of the amino acid distributions at insertion sites of introns that retained their positions

203 The insertion sites of only a tiny fraction of the thousands

204 :H7 strains were tested for the presence and insertion sites of Shiga toxin gene (stx)-containing bac

205 nd quantify the abundance of > 91,000 unique insertion sites of the provirus from 61 HTLV-1(+) person

206 The insertion sites of the Stx-encoding bacteriophages diffe

207 ximately 2 x 10(5) unique de novo transposon insertion sites of the transposon Hermes in the Saccharo

208 l deletions that were roughly bounded by the insertion sites of the two P elements used.

209 Insertion sites of transposed Ds-ATag elements were iden

210 ing, we devised a technique to determine the insertion sites of virtually all members of the human-sp

211 lving five contiguous genes at the transgene insertion site on chromosome 12C3.

212 The impact of catheter insertion site on infection risk remains controversial.

213 A minimum of 15 mtDNA insertion sites on nine chromosomes were detectable usin

214 l approach, we investigated various feasible insertion sites on the G protein of VSV (VSV-G) for disp

215 Mapping of the insertion sites onto the crystal structure of gB730 sugg

216 positioning itself either deeply in the pre-insertion site or on the crowded evolving minor groove s

217 lysis and PCR to detect transposable element insertion site polymorphism in a panel of diverse maize

218 Insertion-site preference at the CAN1 locus requires Ty1

219 Our characterization of the insertion site preferences of Hermes not only assists in

220 We have characterized genome-wide insertion site preferences of piggyBac by sequencing a l

221 heir application, we conducted a genome-wide insertion site profiling of the piggyBac (PB), Tol2 and

222 e structural adaptation to insertions is the insertion site rather than the sequence of the insertion

223 utured either 7 mm posterior to its original insertion site (recession surgery) or at the same site (

224 cing allows affordable ultra-deep transposon insertion site recovery in high-throughput formats withi

225 rated that IS5 can precisely excise from its insertion site, restoring the wild-type phenotype.

226 Pol III transcribed genes; alignment of Ty1 insertion sites revealed a strong sequence motif centere

227 ct, high-throughput sequencing of transposon insertion sites revealed fitness phenotypes of a bank of

228 examination of 137,410 somatic and germinal insertion sites revealed that 50% of the tagged genes ha

229 equent cause for complex circuits, and their insertion site(s) can be targeted for ablation.

230 block was achieved in 75% by targeting these insertion site(s).

231 n of the Shiga toxin bacteriophage and their insertion sites (SBI typing) revealed that cattle and sh

232 gations is to elucidate features influencing insertion site selection.

233 the R2 ribozyme is strongly modulated by the insertion site sequence in the rDNA, with the most commo

234 Analysis Tool) for the efficient analysis of insertion site sequence reads against vertebrate and inv

235 combinations of endonuclease specificity and insertion site sequences in a biological host.

236 ibe a method for semiquantitative transposon insertion site sequencing (QiSeq).

237 We used transposon insertion site sequencing (Tn-seq) to comprehensively as

238 uman gut epithelial cells was assessed by Tn-insertion site sequencing (Tn-seq).

239 We applied a transposon-directed insertion site sequencing (TraDIS) strategy to investiga

240 We used transposon-directed insertion site sequencing (TraDIS) to retrospectively an

241 We have used transposon-directed insertion site sequencing (TraDIS) to screen mutants of

242 son sequencing (Tn-Seq), transposon-directed insertion site sequencing (TraDIS), insertion sequencing

243 ' approach consisting of transposon-directed insertion site sequencing (TraDIS), RNA-seq transcriptom

244 Using transposon-directed insertion site sequencing (TraDIS), we determined differ

245 virulent GBS strain and transposon-directed insertion site sequencing (TraDIS), we performed genome-

246 ify virulence loci using transposon-directed insertion site sequencing (TraDIS).

247 we carried out a high-throughput, transposon insertion site sequencing analysis (TnSeq) to identify g

248 We therefore used transposon-directed insertion site sequencing to identify the complete set o

249 present ChlaMmeSeq (Chlamydomonas MmeI-based insertion site Sequencing), a tool for simultaneous mapp

250 Transposon directed insertion-site sequencing (TraDIS) is a powerful tool to

251 lleagues have utilized a transposon-directed insertion-site sequencing (TraDIS) protocol to identify

252 Using transposon-directed insertion-site sequencing (TraDIS), we identified 126 an

253 nem or ciprofloxacin) by Transposon Directed Insertion-site Sequencing (TraDIS).

254 lly, we demonstrate that transposon-directed insertion-site sequencing is not only applicable to the

255 ansposon mutagenesis and transposon-directed insertion-site sequencing to determine the set of genes

256 In this study, we use transposon-directed insertion-site sequencing to probe differences in gene r

257 Transposon insertion-site sequencing was used to analyze the fitnes

258 new version of "TraDIS" (transposon directed insertion-site sequencing) that we term "TraDIS-Xpress"

259 We here describe quantitative insertion-site sequencing, or QIseq, which uses custom l

260 used genome-wide transposon mutagenesis and insertion-site sequencing, RNA-Seq, plus mass spectromet

261 We use our tagged chromosomal insertion site system to identify small sequences from b

262 number of unique lengths of amplicons of an insertion site tends to increase according to its abunda

263 sease results from a larger number of unique insertion sites than in asymptomatic carriers and not, a

264 rates that we have saturated the full set of insertion sites that are actively targeted by Tf1.

265 We identified 43 common proviral insertion sites that contain candidate genes involved in

266 nt and coincided with preferential P-element insertion sites that harbor transcription initiation act

267 alysis of 85 tumors, we identified 77 common insertion sites that map to 56 genes potentially driving

268 nd seven permissive zinc finger domain (ZFD) insertion sites throughout Gag-Pol, including within p12

269 Here, we used a transposon insertion site (TIS) sequencing-based strategy to identi

270 ory sequences, we mapped the H-2Z1 transgene insertion site to chromosome 17, 100 and 460 kb away fro

271 We used information about the transgene insertion site to develop a polymerase chain reaction ge

272 t that it is able to disseminate from a skin insertion site to infect multiple organs.

273 Global, regional, and local insertion site trends were examined.

274 ely sequenced rice genome to determine 1,664 insertion sites using high-throughput sequencing of 24 i

275 The ACL-tibial insertion site was constant at the junction of the anter

276 The transposon insertion site was identified for 29 of the 150 mutants

277 Vein diameter at the PICC insertion site was measured using ultrasound with in-bui

278 trasound, a radiologist's marking the needle insertion site was not associated with decreased pneumot

279 g MreB filaments are needed to coordinate PG insertion sites, we find that local coordination of enzy

280 city of RET2 and the purine-rich nature of U insertion sites, we propose that the distributive +1 ins

281 no acids that immediately flanked the intein insertion site were randomized.

282 Similar chromosomal phage insertion sites were also identified in all 52 PVL-harbo

283 Ty1 insertion sites were also mapped in four mutant lines th

284 ated with a variety of genomic features: (1) Insertion sites were correlated with regular nucleosome

285 Tumor insertion sites were enriched in recurrent somatic copy-

286 tropy reductions near microdialysis catheter insertion sites were highly correlated with reductions i

287 ransposon mutagenesis screen in which common insertion sites were identified in tumors that were prod

288 rocytoma tissue was extracted and transposon insertion sites were identified.

289 As validation, several insertion sites were introduced into a wild-type clone,

290 Four of these insertion sites were not included in the solved structur

291 Large pools of selected insertion sites were sequenced in a high throughput mann

292 femoral vein (in a 1:1:1 ratio if all three insertion sites were suitable [three-choice scheme] and

293 le until the replication fork arrives at the insertion site, whereupon the FD is rapidly degraded.

294 mutant phenotype represent candidate linked insertion sites, which are then confirmed by PCR.

295 on, in particular attenuating the effects of insertions sites, which can result in variations in phen

296 Furthermore, by combining the identified insertion sites with expression quantification, we show

297 tational pipeline, TIPseqHunter, to identify insertion sites with high precision and reliability.

298 in (NP)-specific artificial microRNA from an insertion site within the non-structural (NS) gene segme

299 gamma-globin intron by optimizing the intron insertion site within the reporter gene.

300 ls revealed significantly less clustering of insertion sites within LMO2, MECOM, and other lymphoid p