ライフサイエンスコーパス: insertional

1 did not find evidence that AAV vectors cause insertional activation of oncogenes and subsequent tumor

2 transfer using gammaretroviral vectors, but insertional activation of proto-oncogenes contributed to

3 islands, thereby reducing the potential for insertional activation.

4 s we quantitatively recapitulate patterns of insertional activity that are usually described in quali

5 Furthermore, a transfer (T)-DNA insertional alg3-T2 mutation, which causes accumulation

6 essential for myelopoiesis, we identified an insertional allele hi1727, which disrupts the gene encod

7 ith roles in auxin response, we screened mpk insertional alleles and identified mpk1-1 as a mutant th

8 MAS2 is a single-copy gene whose insertional alleles are embryonic lethal.

9 Transfer DNA insertional alleles that completely eliminate the accumu

10 The identities of mur5 and two SALK insertional alleles were confirmed by allelism tests and

11 force and the relative contributions of the insertional and appositional components, however, are un

12 sis to obtain the relative magnitudes of the insertional and appositional contributions to the rib di

13 o simulate, respectively, the effects of the insertional and appositional forces, and the rib traject

14 y of these retroelements commonly results in insertional and post-insertional mechanisms of genomic i

15 A T-DNA insertional atos9-t mutation blocks the degradation of a

16 o the human reference, we find a significant insertional bias (3:1) in regions corresponding to compl

17 ecific effect of foamy virus, independent of insertional bias, contributing to reduced genotoxicity.

18 dentify genes required for a functional CCM, insertional Bleomycin resistance (Ble(R)) mutants were g

19 The generation of a zebrafish insertional cardiac mutant collection shall facilitate t

20 ntain low amounts of Asc, we searched for an insertional Chlamydomonas reinhardtii mutant affected in

21 tate cancers can be generated either through insertional chromosomal rearrangement or by intrachromos

22 The editing can be insertional, deletional, or substitutional and has been

23 Analysis of a previously identified insertional dhx16 mutation shows that the zygotic compon

24 The null allele e resulted from an insertional disruption in the CDS by Sal-PIF, a 3100-bp

25 The insertional disruption of the CD1349 gene resulted in si

26 that were lacking or deficient in GlcCer by insertional disruption or by RNA interference (RNAi) sup

27 nt genomic rearrangements by suppressing the insertional DNA repair pathway.

28 Deletion or insertional duplication of an evolutionarily conserved r

29 The existence of insertional editing makes gene identification difficult

30 ted at a significantly higher frequency than insertional editing sites and that substitutional editin

31 y individual polymorphisms, depending on the insertional environment within the host genome.

32 Taken together, these results indicate that insertional events should be considered as alternatives

33 ory effect of pleural pressure, and that the insertional force contributes 60% of that inspiratory ef

34 e fibres on the ribs into which they insert (insertional force) and the transmission of abdominal pre

35 in which pM92 expression was disrupted by an insertional/frameshift mutation.

36 The T-DNA insertional gain-of-function mutant plant for LTP5 (ltp5

37 es, which led to the isolation of 29 clones (insertional gametocyte-deficient mutants) that fail to f

38 ion of spontaneous tumors with characterized insertional genetic landscapes.

39 t binds CCCTC-binding factor and reduces its insertional genotoxicity.

40 in parallel, strongly suggesting that these insertional inactivation mutations were adaptive.

41 hese have resulted in the direct deletion or insertional inactivation of 13 genes.

42 Insertional inactivation of a gene in this cluster encod

43 Insertional inactivation of a putative integrase gene on

44 Insertional inactivation of the aerobically expressed ni

45 gene encoding an IFN-gamma binding protein, insertional inactivation of the thymidine kinase gene, a

46 n genes formed fusion sequences due to viral insertional integration.

47 We next isolated and characterized insertional knockout mutants of all three isoforms confi

48 However, homozygous insertional knockout mutations or RNAi knock-down constr

49 In the nhx5 nhx6 T-DNA insertional knockouts, the precursors of the 2S albumin

50 Mutants with an insertional lesion in the bslO gene exhibit exaggerated

51 Here we show that an insertional lesion in the sap structural gene results in

52 cis chain length, we identified mutants with insertional lesions in secA2.

53 Sequence-indexed insertional libraries in maize (Zea mays) are fundamenta

54 Here, we constructed a Mutator (Mu) insertional library in the B73 inbred background designa

55 bidopsis (Arabidopsis thaliana) transfer DNA insertional line (trxo1-1) revealed an increase in the s

56 d by the analysis of the corresponding T-DNA insertional lines.

57 nferring resistance, we observed somatic and insertional loss-of-function mutations in transformation

58 nts commonly results in insertional and post-insertional mechanisms of genomic injury.

59 We show this insertional method generates mutant alleles that are rev

60 icotiana tabacum) Tnt1 retrotransposon as an insertional mutagen in potato.

61 Using validation-based insertional mutagenesis (VBIM), we identified family wit

62 endogenous L1s suggests that it is unlikely insertional mutagenesis alone accounts for the Mov10l1(-

63 esis, we generated Cfp4-deficient strains by insertional mutagenesis and by RNA interference.

64 This can lead to genetic damage through insertional mutagenesis and chromosomal rearrangements b

65 hough powerful enhancer elements have caused insertional mutagenesis and clonal dysregulation.

66 g concerns related to persistent expression, insertional mutagenesis and cytotoxicity.

67 Here, we used transposon insertional mutagenesis and deep sequencing (Tnseq) to i

68 experimentally profile Cas9 using randomized insertional mutagenesis and delineate hotspots in the st

69 Insertional mutagenesis and depletion (iMAD) is a geneti

70 eveloped a genetic screening strategy called insertional mutagenesis and depletion (iMAD) that combin

71 e families (L1, Alu and SVA) associated with insertional mutagenesis and disease.

72 tegrating vectors pose risks associated with insertional mutagenesis and disruption of gene expressio

73 L1 can introduce genomic instability via insertional mutagenesis and DNA double-strand breaks, bo

74 r suppressor using mouse transposon-mediated insertional mutagenesis and Drosophila cancer models.

75 red potentially hazardous, as it can lead to insertional mutagenesis and genomic instability.

76 Insertional mutagenesis and genotoxicity, which usually

77 al dominance that could not be attributed to insertional mutagenesis and instead likely resulted from

78 ing gene therapy vectors include the risk of insertional mutagenesis and limited efficacy due to hepa

79 Early trials also highlighted the risk of insertional mutagenesis and oncogene transactivation ass

80 n, a method that is associated with unwanted insertional mutagenesis and positional effects on transg

81 r, their clinical use raises the concerns of insertional mutagenesis and potential oncogenesis due to

82 etrotransposon can damage the genome through insertional mutagenesis and the generation of DNA double

83 for transposon-mediated genome engineering, insertional mutagenesis and the genome-wide mapping of t

84 , association analysis, expression analysis, insertional mutagenesis and transgenic validation, we de

85 integration into the host genome results in insertional mutagenesis and variable position effects.

86 method for gene therapy because the risks of insertional mutagenesis are eliminated provided that a "

87 acity, host immune response, and the risk of insertional mutagenesis are limiting factors and of conc

88 ha(-/-) and DAGLbeta(-/-) mice generated via insertional mutagenesis by gene-trapping with retroviral

89 Here, we show that insertional mutagenesis by the PiggyBac transposon can b

90 Together, these results indicate that SB-insertional mutagenesis can identify high-grade astrocyt

91 These data clarify how insertional mutagenesis can modulate cell proliferation

92 Genome-wide functional profiling by insertional mutagenesis can reveal protein domains essen

93 g capacity, lack of copy number control, and insertional mutagenesis caused by integration into host

94 correction of monogenic diseases without the insertional mutagenesis caused by multisite integration

95 Although L1-induced insertional mutagenesis causes Mendelian disease, their

96 ene therapy for SCID X1 were associated with insertional mutagenesis causing leukemia, because the vi

97 allenge that remains is reducing the risk of insertional mutagenesis due to random insertion by both

98 Using insertional mutagenesis each gene was characterized and

99 ac transposon system for tamoxifen inducible insertional mutagenesis from a stably integrated chromos

100 d donors, leukemia caused by vector-mediated insertional mutagenesis has been reported in some indivi

101 Malignant outcomes from insertional mutagenesis have featured prominently in the

102 to the host genome, transgenesis can lead to insertional mutagenesis if a coding gene or an essential

103 Insertional mutagenesis in a haploid background can disr

104 complicated by acute leukemia as a result of insertional mutagenesis in a high proportion of patients

105 ates tumorigenesis following Sleeping Beauty insertional mutagenesis in a mouse model of melanoma.

106 use Sleeping Beauty (SB) transposon-mediated insertional mutagenesis in a mouse model of pancreatic d

107 We used retroviral insertional mutagenesis in an effort to identify which g

108 tudy describes a tool, Lentihop, for somatic insertional mutagenesis in human cells and uses this sys

109 ee) iPSCs, which avoid the potential risk of insertional mutagenesis in humans.

110 oid leukemias (AMLs) generated by retroviral insertional mutagenesis in Kras(G12D) "knockin" mice wit

111 genous MLL-AF9 and MLL-ENL oncogenes through insertional mutagenesis in primary human hematopoietic s

112 m that can be used for effective large-scale insertional mutagenesis in soybean.

113 matin modification pathways, suggesting that insertional mutagenesis in these genes promoted therapeu

114 Sleeping Beauty insertional mutagenesis in this model led to accelerated

115 zation of 96 gliomas by genome-wide piggyBac insertional mutagenesis in vivo identifies 281 known and

116 Gene-trap insertional mutagenesis is a high-throughput forward gen

117 Insertional mutagenesis is a powerful means of identifyi

118 Insertional mutagenesis is a powerful tool for both forw

119 Insertional mutagenesis is a powerful tool for determini

120 Retroviral insertional mutagenesis is a powerful tool for identifyi

121 ate near genes and regulatory regions; thus, insertional mutagenesis is a substantial risk.

122 However, in some patients, insertional mutagenesis led to leukemia or myelodysplasi

123 created haploid and heterozygous diploid Tn7 insertional mutagenesis libraries in S. uvarum to identi

124 red in HYDA1 gene expression by screening an insertional mutagenesis library for HYDA1 promoter activ

125 r results demonstrate that extensive somatic insertional mutagenesis occurs very early during the dev

126 RISPR-Cas9 reverse-genetics pipeline enabled insertional mutagenesis of 18 of these 20 transcription

127 Insertional mutagenesis of exsM in Bacillus anthracis De

128 Insertional mutagenesis of legume genomes such as soybea

129 with the IR via MxiG(C) T4 lysozyme-mediated insertional mutagenesis of MxiK revealed its orientation

130 Insertional mutagenesis of regions upstream of the chara

131 Here, we developed a transposon for insertional mutagenesis of Rickettsia conorii, isolating

132 Second, the H-2Z1 transgene causes insertional mutagenesis of Tbc1d5 and Satb1, leading to

133 "complex interaction" model was tested with insertional mutagenesis of the Drosophila Hsp70 core pro

134 We show that insertional mutagenesis of the previously uncharacterize

135 and tractable manner, using transposon-based insertional mutagenesis on the background of chronic pha

136 of the encoded CPS and KS, and the impact of insertional mutagenesis on virulence and the plant defen

137 ted in single-gene disease in humans through insertional mutagenesis or aberrant mRNA splicing.

138 Insertional mutagenesis or RNA-mediated silencing of Os-

139 use of viral vectors prone to silencing and insertional mutagenesis or the use of nonhuman genes.

140 cancers in mice using a transposon-mediated insertional mutagenesis protocol.

141 Disruption of chiA by insertional mutagenesis resulted in cells that failed to

142 r MPXV infection, we performed a genome-wide insertional mutagenesis screen in human haploid cells.

143 Here we developed a novel whole-body insertional mutagenesis screen in mice, which was design

144 ducted a Sleeping Beauty transposon-mediated insertional mutagenesis screen in Rassf1a-null mice to i

145 ansform mammary epithelial cells, we used an insertional mutagenesis screen on cells isolated from wi

146 67 stable transgenic lines generated from an insertional mutagenesis screen using a transposon-based

147 To identify these genes, we performed an insertional mutagenesis screen using the Sleeping Beauty

148 An insertional mutagenesis screen was performed by intercro

149 Using a gene-trap insertional mutagenesis screen, we identified poliovirus

150 In a large-scale zebrafish insertional mutagenesis screen, we identified the pinbal

151 ets of new cancer genes through a pancreatic insertional mutagenesis screen.

152 f rapamycin (ztor) mutant identified from an insertional mutagenesis screen.

153 recent major advances in transposon-mediated insertional mutagenesis screens and compare this technol

154 Yet, retroviral insertional mutagenesis screens identify RUNX genes as c

155 nal annotation of cancer genomes by enabling insertional mutagenesis screens in higher eukaryotes tha

156 Insertional mutagenesis screens in mice are used to iden

157 t to identify DNA integration sites, such as insertional mutagenesis screens, gene and enhancer trap

158 cell proliferative capacity without risk of insertional mutagenesis should have broad utility in dis

159 We demonstrate that the insertional mutagenesis system based on Tnt1 and the 523

160 id piggyBac/Sleeping Beauty transposon-based insertional mutagenesis system that can be mobilized by

161 dology with a tight integration of gene-trap insertional mutagenesis testing and systems biology to i

162 cribe here a large series of mouse lines for insertional mutagenesis that are compatible with two tra

163 selection and also explain the high risk of insertional mutagenesis that is associated with gene the

164 Here we used piggyBac (PB) transposon insertional mutagenesis to anticipate resistance mechani

165 We used insertional mutagenesis to develop a screening method to

166 Here we combined retroviral and transposon insertional mutagenesis to enable cancer gene discovery

167 e unicellular green alga Chlamydomonas using insertional mutagenesis to find mutants that conferred h

168 We have used insertional mutagenesis to identify a new locus, IDA8/BO

169 In this study, we used retroviral insertional mutagenesis to identify genes that accelerat

170 n in Chinese hamster ovary (CHO) cells using insertional mutagenesis to identify genes that regulate

171 used genome-wide CRISPR libraries as well as insertional mutagenesis to identify synthetic viable (ge

172 used an unbiased method for validation-based insertional mutagenesis to isolate a quinacrine-resistan

173 Here, we recruited retroviral insertional mutagenesis to obtain induction of an arbitr

174 sposase alleles to allow transposon-mediated insertional mutagenesis to occur.

175 ned the assay with Agrobacterium tumefaciens insertional mutagenesis to screen for hyphal mutants.

176 y (SB) transposon system has been used as an insertional mutagenesis tool to identify novel cancer ge

177 Insertional mutagenesis using engineered transposons is

178 w drivers of intestinal cancer, we performed insertional mutagenesis using the Sleeping Beauty transp

179 Insertional mutagenesis using transfer DNA or transposab

180 6a12 hypomorphic rat generated by transposon insertional mutagenesis was characterized using RT-PCR,

181 Here, transposon-mediated insertional mutagenesis was used to create 5 libraries o

182 te cellular promoters pose a reduced risk of insertional mutagenesis when compared with vectors with

183 study provides a new mechanism of retrovirus insertional mutagenesis whereby spatial chromatin organi

184 could be modified to drive transposon-based insertional mutagenesis wherever tissue-specific Cre exp

185 We performed insertional mutagenesis with the MOL4070LTR retrovirus i

186 ful tools for creating transgenic organisms, insertional mutagenesis, and genome engineering.

187 ta suggest that ALV-J induces oncogenesis by insertional mutagenesis, and integrations in the MET onc

188 y-one gene studies using positional cloning, insertional mutagenesis, antisense morpholino oligonucle

189 nt populations on par with those produced by insertional mutagenesis, but systematic cataloguing of m

190 with the cellular receptors, we implemented insertional mutagenesis, carbohydrate shielding, and ala

191 etic manipulation including genetic crosses, insertional mutagenesis, chemical mutagenesis, homologou

192 To deal with potential insertional mutagenesis, the vector integrations are the

193 Owing to the risk of insertional mutagenesis, viral transduction has been inc

194 Using piggyBac transposon-mediated insertional mutagenesis, we screened for parasites that

195 erapy strategies, e.g., immune rejection and insertional mutagenesis, which are associated with viral

196 ion of gene expression - carries the risk of insertional mutagenesis, which can lead to activation of

197 olet (UV) light and chemical mutagens, or by insertional mutagenesis.

198 ineages and modifying the cellular genome by insertional mutagenesis.

199 for therapeutic application due to potential insertional mutagenesis.

200 ant near-haploid KBM-7 cells using gene-trap insertional mutagenesis.

201 nthesis was identified by bioinformatics and insertional mutagenesis.

202 ld pose a risk for neoplasia, independent of insertional mutagenesis.

203 ing data for 97 tumors induced by retroviral insertional mutagenesis.

204 -causing mutations and eliminate the risk of insertional mutagenesis.

205 coding gene cluster, which was identified by insertional mutagenesis.

206 ntegrating lentivector to reduce the risk of insertional mutagenesis.

207 d catalog of common and rare variants due to insertional mutagenesis.

208 MKL mouse model generated through retroviral insertional mutagenesis.

209 ten causes variable transgene expression and insertional mutagenesis.

210 somatic mosaicism during neurodevelopment by insertional mutagenesis.

211 viding cells, exhibiting a decreased risk of insertional mutagenesis.

212 screen of mutant mice generated by piggyBac insertional mutagenesis.

213 Here, we performed a large-scale insertional mutagenic screen of injury-resistant podocyt

214 Retrotransposons are "copy-and-paste" insertional mutagens that substantially contribute to ma

215 The use of transposons as insertional mutagens to identify cancer genes in mice ha

216 Of these five, only insertional mutant alleles of RGP2, a gene that encodes

217 ome position of the flanking sequence of the insertional mutant CAL027_01_20 was used to identify the

218 An axy9 insertional mutant exhibits severe growth defects and co

219 f a million flanking sequence tags from rice insertional mutant libraries are publicly available.

220 Screening of a Chlamydomonas insertional mutant library identified a strain strongly

221 In a screen of an insertional mutant library of all 14 annotated PTS perme

222 a-3 fatty acids was isolated by screening an insertional mutant library using gas chromatography-base

223 a screen for liver defects from a zebrafish insertional mutant library, we isolated the mutant cdipt

224 osable Element from Nicotiana tabacum (Tnt1) insertional mutant line were carried out in the one M. t

225 munolocalization, characterization of a tnt1 insertional mutant line, and synchrotron-based X-ray flu

226 Three independent T-DNA insertional mutant lines deficient in 6-phosphofructo-2-

227 In this study, two independent transfer DNA insertional mutant lines of tim17-1 exhibited a germinat

228 Like the th2-1 mutant, an insertional mutant of At5g32470 accumulated ThMP, and th

229 We report that a commonly used insertional mutant of FoxP3 (GFP-Foxp3) modified its mol

230 The RNA interference lines and the T-DNA insertional mutant of OsbZIP48 showed seedling-lethal ph

231 Finally, we present a new mouse insertional mutant of Oxr1, confirming that specific dis

232 In this study, we identified an insertional mutant of the wall teichoic acid (WTA) synth

233 Altering Gle1 using an insertional mutant or antisense morpholinos results in m

234 NA tagged transformants from the Taiwan Rice Insertional Mutant resource.

235 The analysis of an insertional mutant revealed that the acyltransferase slr

236 Surprisingly, the bin2bil1bil2 triple T-DNA insertional mutant still responds to BR and accumulates

237 terization of pCRY was performed based on an insertional mutant that expresses only 11% of the wild-t

238 We generated an analogous insertional mutant to confirm the phenotype and characte

239 An insertional mutant, nrr1-1, accumulates only 50% of the

240 pression is significantly reduced in an acry insertional mutant.

241 ing early stages of development in the T-DNA insertional mutant.

242 In this study, we isolated AGO2 and AGO3 insertional mutants and confirmed that AGO3 is more abun

243 Our analysis of drp2 insertional mutants demonstrates that, similar to the DR

244 Similar to mpk1-1 and mkk3-1 mutants, rbk1 insertional mutants display auxin hypersensitivity, cons

245 Rice researchers make extensive use of insertional mutants for the study of gene function.

246 We present a new tool with which to produce insertional mutants in P. patens in a rapid and straight

247 ed in IRT1 degradation, we screened a set of insertional mutants in RING-type E3 ligases and identifi

248 protein trap gene-breaking transposon (GBT) insertional mutants in the zebrafish, Danio rerio.

249 ZInC is a web-searchable interface of insertional mutants in zebrafish.

250 Through examining all available T-DNA insertional mutants of Arabidopsis MCTPs, we further rev

251 of nonphotosynthetic (acetate-requiring) DNA insertional mutants of Chlamydomonas reinhardtii and iso

252 Transfer DNA insertional mutants of CML38 show an enhanced sensitivit

253 al targets for new therapeutics, we screened insertional mutants of T. gondii for a reduced ability t

254 e, we report characterization of three T-DNA insertional mutants of the gene encoding cytochrome b5 r

255 We identified 14 independent T. gondii insertional mutants out of >8000 screened that share a d

256 on and activation model to identify parasite insertional mutants that have a fitness defect in infect

257 he large-scale generation of a population of insertional mutants that have been screened for phenotyp

258 the largest phenotyped collections of algal insertional mutants to date.

259 tion of homozygous lethal OXA2a transfer-DNA insertional mutants using the developmentally regulated

260 Xyloglucan from bgal10 insertional mutants was found to contain a large proport

261 In this study, insertional mutants were created and screened to identif

262 complementing homozygous lethal OXA2b T-DNA insertional mutants with a C-terminally truncated OXA2b

263 The analysis of insertional mutants, amiRNA lines and 35S:ERF overexpres

264 Insertional mutants, pdat1-1 and pdat1-2, accumulate 25%

265 insertions and make it easier to select for insertional mutants, we have developed a two-component s

266 er-represented by sixfold among the screened insertional mutants, with insertion events isolated in o

267 e suppression, RNAi, and characterization of insertional mutants.

268 hondrial enzymes in valine degradation using insertional mutants.

269 hich the paramutant allele was induced by an insertional mutation and displayed the "white-tail-tip"

270 Craniofacial defects in mice carrying an insertional mutation in Dlgh-1 suggest that Dlgh-1 is re

271 cits seen in mutants lacking TBs; and 2) the insertional mutation in shk mice is sufficient to produc

272 A transfer-DNA insertional mutation in the cdkg1 gene leads to a temper

273 We used ClosTron to construct an insertional mutation in the dnaK gene of C. difficile 63

274 the future, ZInC may include data from other insertional mutation projects as well.

275 antisense morpholino oligonucleotides and an insertional mutation to inhibit its function during earl

276 An insertional mutation within the LTO1 promoter is associa

277 Among these loci, insertional mutations in aspA, aspB, and sodB resulted i

278 henotypes of C. difficile strains containing insertional mutations in either cwp84 or its paralog cwp

279 he UniformMu transposon resource to identify insertional mutations in vitamin pathway genes.

280 Insertional mutations resulted in embryo lethality that

281 In contrast, transfer DNA insertional null allele of the CAC1A gene is embryo leth

282 Insertional oncogene activation and aberrant splicing ha

283 in limb development, and represent the first insertional phenotype to be cloned in amphibians.

284 emonstrated that they show various levels of insertional polymorphism in mule deer individuals.

285 Thus, P-MLV ERVs show more insertional polymorphism than X-MLVs, and these differen

286 This identified numerous novel human L1(Ta) insertional polymorphisms with highly variant allelic fr

287 These observations suggest that distinct insertional preferences were primary factors that result

288 Beyond revealing interesting properties of insertional RNA editing in Myxomycetes, our work suggest

289 y, we show that the codon position bias in C insertional RNA editing of these two organisms is correl

290 nd sequence diversity of novel insertions by insertional-scanning mutagenesis (InScaM).

291 associated virus (AAV) vectors of autonomous insertional sequences (payloads) encoding diverse functi

292 essential genes using a transposon-directed insertional sequencing (TraDIS) approach.

293 not explained solely by foamy virus' modest insertional site preference for nongenic regions compare

294 Analysis of an insertional T-DNA mutant within the REME1 coding sequenc

295 Insertional therapies have shown great potential for com

296 tructural genes showed the highest degree of insertional tolerance.

297 ed light-harvesting antenna size3 (tla3) DNA insertional transformant of Chlamydomonas reinhardtii is

298 ations (8.4%), adjacent duplications (4.2%), insertional translocations (2.5%), or other complex rear

299 uch as triplications, inverted duplications, insertional translocations, and chromothripsis.

300 An insertional transposon mutation in the sll0606 gene was