ライフサイエンスコーパス: insertional mutagenesis

1 ntegrating lentivector to reduce the risk of insertional mutagenesis.

2 coding gene cluster, which was identified by insertional mutagenesis.

3 ten causes variable transgene expression and insertional mutagenesis.

4 d catalog of common and rare variants due to insertional mutagenesis.

5 MKL mouse model generated through retroviral insertional mutagenesis.

6 erating mutations were induced by retroviral insertional mutagenesis.

7 ding the putative adhesins were disrupted by insertional mutagenesis.

8 a (SREBP-1a) was specifically inactivated by insertional mutagenesis.

9 ro and in vivo with any organism amenable to insertional mutagenesis.

10 episome in replicating cells while avoiding insertional mutagenesis.

11 ncorporate weaker enhancer elements to avoid insertional mutagenesis.

12 odifications may protect coding regions from insertional mutagenesis.

13 f their discovery as an endogenous source of insertional mutagenesis.

14 d forward genetic screening using transposon insertional mutagenesis.

15 ing strain M101, created by single-crossover insertional mutagenesis.

16 ple proviral integrations pose the danger of insertional mutagenesis.

17 encing, likely to protect the germ line from insertional mutagenesis.

18 en using a combination of DNA sequencing and insertional mutagenesis.

19 entified 55 mutants using either chemical or insertional mutagenesis.

20 somatic mosaicism during neurodevelopment by insertional mutagenesis.

21 cells, likely to protect the germ line from insertional mutagenesis.

22 s carries with it a finite chance of causing insertional mutagenesis.

23 al negative effect of transgene products and insertional mutagenesis.

24 ing, aberrant transcriptional regulation and insertional mutagenesis.

25 years since the completion of a large-scale insertional mutagenesis.

26 disease can be corrected without the risk of insertional mutagenesis.

27 stable transgene expression while minimizing insertional mutagenesis.

28 his system substantially reduces the risk of insertional mutagenesis.

29 to minimize the deleterious consequences of insertional mutagenesis.

30 as well as the reported toxicity related to insertional mutagenesis.

31 ntified through oncogenic-retrovirus-induced insertional mutagenesis.

32 ons in mammalian genomes that are capable of insertional mutagenesis.

33 sland was cloned, sequenced and subjected to insertional mutagenesis.

34 eletions, which may lead to loss-of-function insertional mutagenesis.

35 tion, similar to retroviral gain-of-function insertional mutagenesis.

36 reduced vector-related toxicity and without insertional mutagenesis.

37 to design better strategies for genome-wide insertional mutagenesis.

38 cerns about the potential for rAAV2-mediated insertional mutagenesis.

39 vation of embC in Mycobacterium smegmatis by insertional mutagenesis.

40 a Chlamydomonas motility mutant obtained by insertional mutagenesis.

41 igh-level expression and reduce the risk for insertional mutagenesis.

42 e through a variety of mechanisms, including insertional mutagenesis.

43 ineages and modifying the cellular genome by insertional mutagenesis.

44 viding cells, exhibiting a decreased risk of insertional mutagenesis.

45 nthesis was identified by bioinformatics and insertional mutagenesis.

46 screen of mutant mice generated by piggyBac insertional mutagenesis.

47 olet (UV) light and chemical mutagens, or by insertional mutagenesis.

48 for therapeutic application due to potential insertional mutagenesis.

49 ant near-haploid KBM-7 cells using gene-trap insertional mutagenesis.

50 ld pose a risk for neoplasia, independent of insertional mutagenesis.

51 ing data for 97 tumors induced by retroviral insertional mutagenesis.

52 -causing mutations and eliminate the risk of insertional mutagenesis.

53 There is no evidence for insertional mutagenesis after 21-36 months of observatio

54 to study the mechanisms of insertions of the insertional mutagenesis agents and to design better stra

55 endogenous L1s suggests that it is unlikely insertional mutagenesis alone accounts for the Mov10l1(-

56 esis, we generated Cfp4-deficient strains by insertional mutagenesis and by RNA interference.

57 This can lead to genetic damage through insertional mutagenesis and chromosomal rearrangements b

58 hough powerful enhancer elements have caused insertional mutagenesis and clonal dysregulation.

59 g concerns related to persistent expression, insertional mutagenesis and cytotoxicity.

60 Here, we used transposon insertional mutagenesis and deep sequencing (Tnseq) to i

61 experimentally profile Cas9 using randomized insertional mutagenesis and delineate hotspots in the st

62 Insertional mutagenesis and depletion (iMAD) is a geneti

63 eveloped a genetic screening strategy called insertional mutagenesis and depletion (iMAD) that combin

64 e families (L1, Alu and SVA) associated with insertional mutagenesis and disease.

65 tegrating vectors pose risks associated with insertional mutagenesis and disruption of gene expressio

66 L1 can introduce genomic instability via insertional mutagenesis and DNA double-strand breaks, bo

67 r suppressor using mouse transposon-mediated insertional mutagenesis and Drosophila cancer models.

68 red potentially hazardous, as it can lead to insertional mutagenesis and genomic instability.

69 Insertional mutagenesis and genotoxicity, which usually

70 al dominance that could not be attributed to insertional mutagenesis and instead likely resulted from

71 ing gene therapy vectors include the risk of insertional mutagenesis and limited efficacy due to hepa

72 Early trials also highlighted the risk of insertional mutagenesis and oncogene transactivation ass

73 n, a method that is associated with unwanted insertional mutagenesis and positional effects on transg

74 r, their clinical use raises the concerns of insertional mutagenesis and potential oncogenesis due to

75 ecific integration events that may result in insertional mutagenesis and reduced fitness.

76 etrotransposon can damage the genome through insertional mutagenesis and the generation of DNA double

77 for transposon-mediated genome engineering, insertional mutagenesis and the genome-wide mapping of t

78 new insights into the risk of rAAV-mediated insertional mutagenesis and the mechanisms of rAAV integ

79 , association analysis, expression analysis, insertional mutagenesis and transgenic validation, we de

80 integration into the host genome results in insertional mutagenesis and variable position effects.

81 ful tools for creating transgenic organisms, insertional mutagenesis, and genome engineering.

82 ta suggest that ALV-J induces oncogenesis by insertional mutagenesis, and integrations in the MET onc

83 for transcriptional activation by retroviral insertional mutagenesis, and the oncogenic potential of

84 The ylxL gene was disrupted by insertional mutagenesis, and the resultant mutant strain

85 y-one gene studies using positional cloning, insertional mutagenesis, antisense morpholino oligonucle

86 method for gene therapy because the risks of insertional mutagenesis are eliminated provided that a "

87 acity, host immune response, and the risk of insertional mutagenesis are limiting factors and of conc

88 nd helps define optimal strategies for using insertional mutagenesis as a genomic tool.

89 A clc-a mutant, Mlac3, generated by insertional mutagenesis as well as a targeted Deltaclc-a

90 olation of mutants by classical genetics and insertional mutagenesis, as well as by the disruption of

91 ment potential of CD34+ progenitor cells via insertional mutagenesis at this specific locus.

92 nt populations on par with those produced by insertional mutagenesis, but systematic cataloguing of m

93 The use of insertional mutagenesis by Agrobacterium has uncovered a

94 ha(-/-) and DAGLbeta(-/-) mice generated via insertional mutagenesis by gene-trapping with retroviral

95 Insertional mutagenesis by retroviral vectors is a major

96 tracellular domain of SR-BI was subjected to insertional mutagenesis by strategically placing an epit

97 Here, we show that insertional mutagenesis by the PiggyBac transposon can b

98 Genes identified by insertional mutagenesis by their nature could also be in

99 Here we show that insertional mutagenesis can also identify genes that pro

100 n that sleeping beauty (SB) transposon-based insertional mutagenesis can also identify novel candidat

101 Together, these results indicate that SB-insertional mutagenesis can identify high-grade astrocyt

102 These data clarify how insertional mutagenesis can modulate cell proliferation

103 Genome-wide functional profiling by insertional mutagenesis can reveal protein domains essen

104 up-regulated, demonstrating that retroviral insertional mutagenesis can target miRNA loci as well as

105 with the cellular receptors, we implemented insertional mutagenesis, carbohydrate shielding, and ala

106 g capacity, lack of copy number control, and insertional mutagenesis caused by integration into host

107 correction of monogenic diseases without the insertional mutagenesis caused by multisite integration

108 Although L1-induced insertional mutagenesis causes Mendelian disease, their

109 ene therapy for SCID X1 were associated with insertional mutagenesis causing leukemia, because the vi

110 On the other hand, insertional mutagenesis causing liver cancer has been im

111 etic manipulation including genetic crosses, insertional mutagenesis, chemical mutagenesis, homologou

112 allenge that remains is reducing the risk of insertional mutagenesis due to random insertion by both

113 Using insertional mutagenesis each gene was characterized and

114 To assess whether this insertional mutagenesis event results in alteration of t

115 These studies establish a profile of insertional mutagenesis for AAV vectors and provide uniq

116 ac transposon system for tamoxifen inducible insertional mutagenesis from a stably integrated chromos

117 Insertional mutagenesis generated avirulent isolates of

118 L1 expression has been shown to result in insertional mutagenesis, genomic deletions and rearrange

119 d donors, leukemia caused by vector-mediated insertional mutagenesis has been reported in some indivi

120 Although retroviral insertional mutagenesis has proven to be an effective to

121 cal occurrence of T cell malignancies due to insertional mutagenesis has raised concerns about the sa

122 Malignant outcomes from insertional mutagenesis have featured prominently in the

123 to the host genome, transgenesis can lead to insertional mutagenesis if a coding gene or an essential

124 SCID) gene therapy that increase the risk of insertional mutagenesis; (ii) what other genetic lesions

125 Insertional mutagenesis in a haploid background can disr

126 complicated by acute leukemia as a result of insertional mutagenesis in a high proportion of patients

127 ates tumorigenesis following Sleeping Beauty insertional mutagenesis in a mouse model of melanoma.

128 use Sleeping Beauty (SB) transposon-mediated insertional mutagenesis in a mouse model of pancreatic d

129 We used retroviral insertional mutagenesis in an effort to identify which g

130 The combination of insertional mutagenesis in Blm-deficient ES cells establ

131 urther exploring the risk factors leading to insertional mutagenesis in gene therapy trials.

132 tudy describes a tool, Lentihop, for somatic insertional mutagenesis in human cells and uses this sys

133 ee) iPSCs, which avoid the potential risk of insertional mutagenesis in humans.

134 screen for potential tumor suppressors using insertional mutagenesis in immortalized fibroblasts.

135 oid leukemias (AMLs) generated by retroviral insertional mutagenesis in Kras(G12D) "knockin" mice wit

136 in a recent issue of Nature now reveals that insertional mutagenesis in mammalian cells is possible t

137 ion efficiency, recent reports of retroviral insertional mutagenesis in mice and two human subjects h

138 Insertional mutagenesis in mice demonstrates that Rfx4_v

139 deficiency, the UCE gene was inactivated via insertional mutagenesis in mice.

140 Retroviral insertional mutagenesis in mouse hematopoietic tumors pr

141 Retroviral insertional mutagenesis in mouse hematopoietic tumors pr

142 genous MLL-AF9 and MLL-ENL oncogenes through insertional mutagenesis in primary human hematopoietic s

143 We have developed a novel system for insertional mutagenesis in rice (Oryza sativa) based on

144 Thus, electroporation-mediated insertional mutagenesis in S.pombe is preceded by exonuc

145 m that can be used for effective large-scale insertional mutagenesis in soybean.

146 Insertional mutagenesis in the first predicted intermemb

147 DNA insertional mutagenesis in the model organism Chlamydomo

148 matin modification pathways, suggesting that insertional mutagenesis in these genes promoted therapeu

149 Sleeping Beauty insertional mutagenesis in this model led to accelerated

150 zation of 96 gliomas by genome-wide piggyBac insertional mutagenesis in vivo identifies 281 known and

151 hroughout the genome, potentially leading to insertional mutagenesis, inappropriate activation of nea

152 cterizing site selection for integration and insertional mutagenesis indicate that the Sleeping Beaut

153 dentity was identified in S. coelicolor, and insertional mutagenesis indicated that this gene was not

154 n average represent fairly small targets for insertional mutagenesis, indicates the general utility o

155 the deepest rough C. jejuni 81-176 mutant by insertional mutagenesis into the waaC gene, encoding hep

156 Insertional mutagenesis is a cornerstone of functional g

157 Gene-trap insertional mutagenesis is a high-throughput forward gen

158 Insertional mutagenesis is a powerful means of identifyi

159 Insertional mutagenesis is a powerful method for gene di

160 Insertional mutagenesis is a powerful tool for both forw

161 Insertional mutagenesis is a powerful tool for determini

162 Retroviral insertional mutagenesis is a powerful tool for identifyi

163 ate near genes and regulatory regions; thus, insertional mutagenesis is a substantial risk.

164 Drosophila melanogaster, P element-mediated insertional mutagenesis is a versatile tool for the diss

165 Transposon insertional mutagenesis is an effective alternative to T

166 in OPA and add weight to the hypothesis that insertional mutagenesis is involved in the development o

167 To test the hypothesis that JSRV insertional mutagenesis is involved in the oncogenesis o

168 Our results provide strong evidence that insertional mutagenesis is not required for in vitro rep

169 hether mature lymphocytes are susceptible to insertional mutagenesis is unknown.

170 ration in each transformant, indicating that insertional mutagenesis is useful for generating single-

171 However, in some patients, insertional mutagenesis led to leukemia or myelodysplasi

172 created haploid and heterozygous diploid Tn7 insertional mutagenesis libraries in S. uvarum to identi

173 red in HYDA1 gene expression by screening an insertional mutagenesis library for HYDA1 promoter activ

174 tion of multiple insertion sites in a random insertional mutagenesis library.

175 mapping double-insertion events in a random insertional-mutagenesis library.

176 abase for archiving, searching and analyzing insertional mutagenesis lines.

177 developed a web application called iMapper (Insertional Mutagenesis Mapping and Analysis Tool) for t

178 ediated leukemogenesis as well as retroviral insertional mutagenesis mechanisms.

179 e developed an efficient transposon-mediated insertional mutagenesis method much needed for high-thro

180 r results demonstrate that extensive somatic insertional mutagenesis occurs very early during the dev

181 RISPR-Cas9 reverse-genetics pipeline enabled insertional mutagenesis of 18 of these 20 transcription

182 Insertional mutagenesis of Arabidopsis ecotype C24 was u

183 viruses can induce hematopoietic disease via insertional mutagenesis of cancer genes and provide valu

184 ilization likely promotes tumor formation by insertional mutagenesis of cancer genes, and not by prom

185 The tumours result from SB insertional mutagenesis of cancer genes, thus facilitati

186 Insertional mutagenesis of cooperating cancer genes by a

187 Insertional mutagenesis of exsM in Bacillus anthracis De

188 Insertional mutagenesis of legume genomes such as soybea

189 with the IR via MxiG(C) T4 lysozyme-mediated insertional mutagenesis of MxiK revealed its orientation

190 Insertional mutagenesis of nocJ abolished nocardicin A p

191 Insertional mutagenesis of regions upstream of the chara

192 Here, we developed a transposon for insertional mutagenesis of Rickettsia conorii, isolating

193 olecules also may be useful as tools for the insertional mutagenesis of target mRNAs.

194 Second, the H-2Z1 transgene causes insertional mutagenesis of Tbc1d5 and Satb1, leading to

195 functional genomics of photosynthesis using insertional mutagenesis of the Chlamydomonas nuclear gen

196 "complex interaction" model was tested with insertional mutagenesis of the Drosophila Hsp70 core pro

197 We show that insertional mutagenesis of the previously uncharacterize

198 Insertional mutagenesis of the undomesticated 3610 strai

199 We describe and characterize a method for insertional mutagenesis of the yeast pathogen Candida gl

200 and tractable manner, using transposon-based insertional mutagenesis on the background of chronic pha

201 of the encoded CPS and KS, and the impact of insertional mutagenesis on virulence and the plant defen

202 ted in single-gene disease in humans through insertional mutagenesis or aberrant mRNA splicing.

203 Lines in which FHL function was abolished by insertional mutagenesis or attenuated by RNAi-mediated s

204 Insertional mutagenesis or RNA-mediated silencing of Os-

205 ation, as a unique tool for genomic studies (insertional mutagenesis or targeted DNA integration) and

206 use of viral vectors prone to silencing and insertional mutagenesis or the use of nonhuman genes.

207 ive treatment in humans, some concerns about insertional mutagenesis persist.

208 We have developed a high-throughput T-DNA insertional mutagenesis program in tomato using activati

209 cancers in mice using a transposon-mediated insertional mutagenesis protocol.

210 Disruption of chiA by insertional mutagenesis resulted in cells that failed to

211 ow that retroviruses that engender different insertional mutagenesis risks can have similar integrati

212 eted gene disruption or allele substitution, insertional mutagenesis, RNA interference and homologous

213 An insertional mutagenesis screen identified roles for the

214 r MPXV infection, we performed a genome-wide insertional mutagenesis screen in human haploid cells.

215 Here we developed a novel whole-body insertional mutagenesis screen in mice, which was design

216 ducted a Sleeping Beauty transposon-mediated insertional mutagenesis screen in Rassf1a-null mice to i

217 have been used as mutagens for a large-scale insertional mutagenesis screen in the zebra fish.

218 We completed a large insertional mutagenesis screen in zebrafish to identify

219 elopment, we are carrying out a large-scale, insertional mutagenesis screen in zebrafish, using mouse

220 In an insertional mutagenesis screen in zebrafish, we identifi

221 ansform mammary epithelial cells, we used an insertional mutagenesis screen on cells isolated from wi

222 We carried out an insertional mutagenesis screen to isolate bypass suppres

223 67 stable transgenic lines generated from an insertional mutagenesis screen using a transposon-based

224 To identify these genes, we performed an insertional mutagenesis screen using the Sleeping Beauty

225 An insertional mutagenesis screen was performed by intercro

226 ctable marker for nuclear transformation, an insertional mutagenesis screen was performed to select f

227 From an insertional mutagenesis screen we identified a novel gen

228 hat mutations in CKA2, identified through an insertional mutagenesis screen, confer fluconazole resis

229 Recently, a large-scale retroviral insertional mutagenesis screen, in which 315 different g

230 Using a gene-trap insertional mutagenesis screen, we identified poliovirus

231 In a large-scale zebrafish insertional mutagenesis screen, we identified the pinbal

232 f rapamycin (ztor) mutant identified from an insertional mutagenesis screen.

233 ets of new cancer genes through a pancreatic insertional mutagenesis screen.

234 Consistent with these findings, retroviral insertional mutagenesis screening of our T-cell leukemia

235 To manage multiple high-throughput insertional mutagenesis screening projects, we developed

236 recent major advances in transposon-mediated insertional mutagenesis screens and compare this technol

237 Yet, retroviral insertional mutagenesis screens identify RUNX genes as c

238 elements, have been successfully applied for insertional mutagenesis screens in both the mouse and ra

239 nal annotation of cancer genomes by enabling insertional mutagenesis screens in higher eukaryotes tha

240 Insertional mutagenesis screens in mice are used to iden

241 Insertional mutagenesis screens play an integral part in

242 t to identify DNA integration sites, such as insertional mutagenesis screens, gene and enhancer trap

243 cell proliferative capacity without risk of insertional mutagenesis should have broad utility in dis

244 t to this process, we utilized a Tn917-based insertional mutagenesis strategy to generate a mutant ba

245 We have now used an insertional mutagenesis strategy to generate two differe

246 axel resistance in breast cancer, we used an insertional mutagenesis strategy to identify proteins wh

247 essed in meristematic cells, a promoter trap insertional mutagenesis strategy was used in Arabidopsis

248 We demonstrate that the insertional mutagenesis system based on Tnt1 and the 523

249 id piggyBac/Sleeping Beauty transposon-based insertional mutagenesis system that can be mobilized by

250 Insertional mutagenesis techniques, including transposon

251 dology with a tight integration of gene-trap insertional mutagenesis testing and systems biology to i

252 cribe here a large series of mouse lines for insertional mutagenesis that are compatible with two tra

253 that these vectors are an effective tool for insertional mutagenesis that can be used for either gene

254 selection and also explain the high risk of insertional mutagenesis that is associated with gene the

255 To deal with potential insertional mutagenesis, the vector integrations are the

256 Here we used piggyBac (PB) transposon insertional mutagenesis to anticipate resistance mechani

257 We used insertional mutagenesis to develop a screening method to

258 n this study, we have exploited the power of insertional mutagenesis to elucidate tumor progression p

259 Here we combined retroviral and transposon insertional mutagenesis to enable cancer gene discovery

260 e unicellular green alga Chlamydomonas using insertional mutagenesis to find mutants that conferred h

261 nzyme, we performed large scale pentapeptide insertional mutagenesis to generate insertions of five a

262 We have used insertional mutagenesis to identify a new locus, IDA8/BO

263 In this study, we used retroviral insertional mutagenesis to identify genes that accelerat

264 In the present study, we used retroviral insertional mutagenesis to identify genes that might col

265 n in Chinese hamster ovary (CHO) cells using insertional mutagenesis to identify genes that regulate

266 used genome-wide CRISPR libraries as well as insertional mutagenesis to identify synthetic viable (ge

267 used an unbiased method for validation-based insertional mutagenesis to isolate a quinacrine-resistan

268 Here, we recruited retroviral insertional mutagenesis to obtain induction of an arbitr

269 sposase alleles to allow transposon-mediated insertional mutagenesis to occur.

270 ned the assay with Agrobacterium tumefaciens insertional mutagenesis to screen for hyphal mutants.

271 y (SB) transposon system has been used as an insertional mutagenesis tool to identify novel cancer ge

272 otential hazards to genome integrity through insertional mutagenesis, unequal recombination and chrom

273 Insertional mutagenesis using engineered transposons is

274 w drivers of intestinal cancer, we performed insertional mutagenesis using the Sleeping Beauty transp

275 Insertional mutagenesis using transfer DNA or transposab

276 efficient use of lentiviral validation-based insertional mutagenesis (VBIM) to generate large populat

277 Using validation-based insertional mutagenesis (VBIM), we identified family wit

278 ngineered Arabidopsis (Arabidopsis thaliana) insertional mutagenesis vectors that are based on the ma

279 Owing to the risk of insertional mutagenesis, viral transduction has been inc

280 Insertional mutagenesis was achieved by electroporation

281 6a12 hypomorphic rat generated by transposon insertional mutagenesis was characterized using RT-PCR,

282 l importance within proLasA, linker-scanning insertional mutagenesis was employed using a plasmid con

283 Insertional mutagenesis was used to abrogate gene functi

284 Here, transposon-mediated insertional mutagenesis was used to create 5 libraries o

285 Chimeric and insertional mutagenesis was used to generate mutants of

286 Retroviral insertional mutagenesis was used to produce a mutant Chi

287 s of XMRV and the potential risk of proviral insertional mutagenesis, we carried out a genome-wide an

288 Using insertional mutagenesis, we identified a gene, RYP1 (req

289 By using gene trap insertional mutagenesis, we identified Rab9, which media

290 Using insertional mutagenesis, we isolated a UV-sensitive muta

291 Using piggyBac transposon-mediated insertional mutagenesis, we screened for parasites that

292 te cellular promoters pose a reduced risk of insertional mutagenesis when compared with vectors with

293 study provides a new mechanism of retrovirus insertional mutagenesis whereby spatial chromatin organi

294 could be modified to drive transposon-based insertional mutagenesis wherever tissue-specific Cre exp

295 erapy strategies, e.g., immune rejection and insertional mutagenesis, which are associated with viral

296 ion of gene expression - carries the risk of insertional mutagenesis, which can lead to activation of

297 retroid agents are implicated in disease via insertional mutagenesis, while others have been found to

298 Insertional mutagenesis with a heterologous transposon p

299 We describe an improved method for insertional mutagenesis with retroviral vectors and show

300 We performed insertional mutagenesis with the MOL4070LTR retrovirus i