ライフサイエンスコーパス: integrin alphaIIbbeta3

1 a integrin alpha5beta1), and fibrinogen (via integrin alphaIIbbeta3).

2 aIIb subunit of the highly abundant platelet integrin alphaIIbbeta3.

3 d into integrin antagonists for the platelet integrin alphaIIbbeta3.

4 stimulation activates platelet spreading and integrin alphaIIbbeta3.

5 ities in a major platelet adhesion receptor, integrin alphaIIbbeta3.

6 that talin binding is sufficient to activate integrin alphaIIbbeta3.

7 C45 interaction with the cytoplasmic face of integrin alphaIIbbeta3.

8 IX-V activate the ligand-binding function of integrin alphaIIbbeta3.

9 ppears to be a key regulator of the platelet integrin alphaIIbbeta3.

10 SLP-76 is involved in signaling mediated by integrin alphaIIbbeta3.

11 acellular signaling leading to activation of integrin alphaIIbbeta3.

12 and disrupted its physical association with integrin alphaIIbbeta3.

13 In addition, mSpeB2 bound purified platelet integrin alphaIIbbeta3.

14 data had been generated with RGD binding to integrin alphaIIbbeta3.

15 ement of the platelet/megakaryocyte-specific integrin alphaIIbbeta3.

16 t CIB is a candidate regulatory molecule for integrin alphaIIbbeta3.

17 e for their specific binding to the platelet integrin alphaIIbbeta3.

18 in a loss of the ligand binding function of integrin alphaIIbbeta3.

19 b molecules, AP7 and PAC1.1, to the platelet integrin alphaIIbbeta3.

20 platelet adhesion to IgG independent of the integrin alphaIIbbeta3.

21 bunits promote constitutive signaling by the integrin alphaIIbbeta3.

22 ha causes activation of the platelet surface integrin alphaIIbbeta3.

23 on platelet activation by GPVI, CLEC-2, and integrin alphaIIbbeta3.

24 activation, and subsequent activation of the integrin alphaIIbbeta3.

25 ted platelet disorder caused by mutations in integrin alphaIIbbeta3.

26 nactive resting conformation of the platelet integrin alphaIIbbeta3.

27 GD peptides and the extracellular domains of integrin alphaIIbbeta3.

28 ranule protein retention, and lack of active integrin alphaIIbbeta3.

29 eta3 cytosolic tail causes activation of the integrin alphaIIbbeta3.

30 side and blocked talin-induced activation of integrin alphaIIbbeta3.

31 c example of this phenomenon is the platelet integrin, alphaIIbbeta3.

32 th signaling pathways involving the platelet integrin, alphaIIbbeta3.

33 hanisms are based on studies of the platelet integrin, alphaIIbbeta3.

34 elet agonists increase the affinity state of integrin alphaIIbbeta3, a prerequisite for fibrinogen bi

35 Here, we reveal that integrin alphaIIbbeta3, a prototypic adhesion receptor,

36 roposed to follow the structural template of integrin alphaIIbbeta3(A711P).

37 s (PMAs), p-selectin expression (P-SEL), and integrin alphaIIbbeta3 activation (PAC-1 binding) were a

38 s directly bind to TLR2 and induces platelet integrin alphaIIbbeta3 activation and P-selectin express

39 PI3K activation regulates platelet integrin alphaIIbbeta3 activation and platelet aggregati

40 ADAP also promotes integrin alphaIIbbeta3 activation in platelets, which la

41 Integrin alphaIIbbeta3 activation is essential for plate

42 dysfunction in EDS is an outcome of reduced integrin alphaIIbbeta3 activation resulting from decreas

43 intracellular signaling cascades, leading to integrin alphaIIbbeta3 activation, a process antagonized

44 evels of platelet activation, as measured by integrin alphaIIbbeta3 activation, alpha-granule secreti

45 lting in defects in alpha-granule secretion, integrin alphaIIbbeta3 activation, and actin assembly.

46 ses of alpha and dense-granule secretion and integrin alphaIIbbeta3 activation, and aggregation were

47 Platelet aggregation, integrin alphaIIbbeta3 activation, and alpha-granule and

48 and have decreased alpha-granule secretion, integrin alphaIIbbeta3 activation, and protein tyrosine

49 rmsii was capable of triggering platelet and integrin alphaIIbbeta3 activation, as indicated by the e

50 e in GPIb-IX-V function and agonist-mediated integrin alphaIIbbeta3 activation, associated with loss

51 g Bruton's tyrosine kinase (Btk) that led to integrin alphaIIbbeta3 activation, platelet aggregation,

52 let adhesion to collagen in flow conditions, integrin alphaIIbbeta3 activation, washed platelet secre

53 the molecular mechanisms regulating platelet integrin alphaIIbbeta3 activation.

54 ulation of these cells displayed recovery of integrin alphaIIbbeta3 activation.

55 by de novo protein synthesis of alphaIIb and integrin alphaIIbbeta3 activation.

56 d was not mediated by glycoprotein IIb/IIIa (integrin alphaIIbbeta3) activation.

57 ssociated with a shear-dependent increase in integrin alphaIIbbeta3 adhesive function, resulting in e

58 lins, kindlins have little primary effect on integrin alphaIIbbeta3 affinity for monovalent ligands a

59 Integrin alphaIIbbeta3 affinity regulation by talin bind

60 Although agonist-induced integrin alphaIIbbeta3 affinity regulation was unaltered

61 rketed treatments have successfully targeted integrins alphaIIbbeta3, alpha4beta7/alpha4beta1 and alp

62 ey to fibrinogen (Fg) uptake, trafficking of integrins (alphaIIbbeta3, alphavbeta3), and purinergic r

63 e, Borrelia burgdorferi (sensu lato) bind to integrins alphaIIbbeta3, alphavbeta3 and alpha5beta1 in

64 action was promoted by ligand binding to the integrin alphaIIbbeta3 and by guanosine triphosphate (GT

65 n monoclonal antibodies that are specific to integrin alphaIIbbeta3 and can potently inhibit platelet

66 s strongly inhibited the interaction between integrin alphaIIbbeta3 and fibrinogen and platelet aggre

67 This interaction is mediated by platelet integrin alphaIIbbeta3 and requires biomechanical activa

68 receptor-dependent activation of the surface integrin alphaIIbbeta3 and subsequent binding of fibrino

69 procoagulant platelet-associated changes in integrin alphaIIbbeta3 and the physiologic role of proco

70 vation that involves the binding of MMP-2 to integrin alphaIIbbeta3 and the subsequent cleavage of PA

71 a3 integrin-deficient mice (lacking platelet integrin alphaIIbbeta3 and the widely expressed nonplate

72 lpha2beta1 promoted inside-out activation of integrin alphaIIbbeta3 and thrombus formation.

73 rsCD40L specifically bound to purified integrin alphaIIbbeta3 and to activated platelets in a b

74 ognize the TM helices of two closely related integrins (alphaIIbbeta3 and alphavbeta3) in micelles, b

75 hat the failed, small-molecule inhibitors of integrins alphaIIbbeta3 and alpha4beta1 stabilize the hi

76 In the case of integrins alphaIIbbeta3 and alpha5beta1, the integrin bi

77 idues in the alpha-subunits of the two beta3 integrins alphaIIbbeta3 and alphaVbeta3, but a potential

78 an attractive candidate bacterial ligand for integrins alphaIIbbeta3 and alphavbeta3.

79 The integrins, alphaIIbbeta3 and alphaVbeta3, play a key rol

80 phaIIbbeta3: the bacterium bound to purified integrin alphaIIbbeta3, and bacterial binding to platele

81 e phosphorylated after fibrinogen binding to integrin alphaIIbbeta3, and collagen-stimulated platelet

82 egation, expression of the activated form of integrin alphaIIbbeta3, and exposure of phosphatidylseri

83 conformational changes in the major platelet integrin, alphaIIbbeta3, and induce minor changes in pla

84 In addition to integrin alphaIIbbeta3, another as-yet-unidentified rece

85 and thrombosis following treatment with the integrin alphaIIbbeta3 antagonist eptifibatide are rare

86 r PP1, the Syk kinase inhibitor R406 and the integrin alphaIIbbeta3 antagonist lotrafiban.

87 atment of the TTP mice with eptifibatide, an integrin alphaIIbbeta3 antagonist, reduced pulmonary art

88 nding site and a cytoplasmic LIBS epitope in integrin alphaIIbbeta3 are conformationally and function

89 Antagonists of the platelet integrin alphaIIbbeta3 are potent anti-thrombotic drugs,

90 brinogen binding, glycoprotein (GP)IIb-IIIa (integrin alphaIIbbeta3) becomes associated with the cyto

91 tometry (expression of P-selectin and active integrin alphaIIbbeta3 before and after activation), the

92 The platelet integrin alphaIIbbeta3 binds to a KQAGDV motif at the fi

93 d heterodimeric receptors including platelet integrin alphaIIbbeta3 but also alphavbeta3, expressed s

94 V/W)CRAD(K/R)RC) and high specificity toward integrin alphaIIbbeta3 but not to other RGD binding inte

95 glycoprotein Ib (GPIb)-IX-V complex and the integrin alphaIIbbeta3, but this ABO effect is likely on

96 activated from resting conformations of the integrin alphaIIbbeta3 can be regulated by limited amino

97 al modulation of the membrane curvature near integrin alphaIIbbeta3 can induce its activation in cell

98 vascular thrombosis by antagonizing platelet integrin alphaIIbbeta3 cannot be achieved without compro

99 The plasma-membrane integrin alphaIIbbeta3 (CD41/CD61, GPIIbIIIa) is a major

100 In CHO cells expressing integrin alphaIIbbeta3, co-expression of K2 with talin h

101 d that a subpopulation of the major platelet integrin, alphaIIbbeta3, co-sediments from detergent lys

102 Inhibiting integrin alphaIIbbeta3 completely abolishes ChAdOx1-indu

103 Human or mouse platelets lacking functional integrin alphaIIbbeta3 complexes and human platelets pre

104 Integrin alphaIIbbeta3 contains an on/off switch that re

105 earrangements induced by these agents in the integrin alphaIIbbeta3 correlate with its ability to bin

106 r, deficiency of Dok-2 leads to dysregulated integrin alphaIIbbeta3-dependent cytosolic calcium flux

107 As expected, an antibody to the platelet integrin alphaIIbbeta3 did not inhibit endothelial cell-

108 rmined three-dimensional structures of human integrin alphaIIbbeta3 embedded in a lipid bilayer (nano

109 emonstrated that fibrinogen, an activator of integrin alphaIIbbeta3, enhances SERT activity in human

110 mong strongly stimulated adherent platelets, integrin alphaIIbbeta3 epitope changes, mPTP formation,

111 utively active platelet receptor GPIIb/IIIa (integrin alphaIIbbeta3) expressed on Chinese hamster ova

112 Expression of wild-type Rasa3 in integrin alphaIIbbeta3-expressing CHO cells blocked Rap1

113 muscle myosin IIA, actin polymerization, and integrin alphaIIbbeta3-fibrin interactions, indicating t

114 mediate signaling via the platelet-specific integrin alphaIIbbeta3, fibrinogen binding was induced b

115 to select monoclonal antibodies specific to integrin alphaIIbbeta3 from a synthetic human antibody l

116 in a conformational change of the prototype integrin alphaIIbbeta3 from an inactive to an active sta

117 ombinant forms, to platelets and to purified integrins alphaIIbbeta3 (from platelets) and alphaVbeta3

118 nhibition of platelet activation or platelet integrin alphaIIbbeta3 function abolished M21 cell attac

119 In the absence of CypD, integrin alphaIIbbeta3 function was accentuated in both

120 of lipid kinases plays an important role in integrin alphaIIbbeta3 function, thereby supporting thro

121 ne domains are involved in the regulation of integrin alphaIIbbeta3 function.

122 ding onto fibrinogen resulting from enhanced integrin alphaIIbbeta3 function.

123 Integrin alphaIIbbeta3 functions as the fibrinogen recep

124 the cytosolic tails of the adhesion receptor integrin alphaIIbbeta3, fused to a coiled-coil construct

125 he human blood platelet fibrinogen receptor, integrin alphaIIbbeta3 (glycoprotein IIb-IIIa) is an arc

126 ic agents target the interaction of platelet integrin alphaIIbbeta3 (GPIIb-IIIa) with fibrinogen duri

127 Ligand binding to the platelet integrin alphaIIbbeta3 (GPIIb-IIIa), a prerequisite for

128 a Glanzmann's patient, who lacked functional integrin alphaIIbbeta3 (GPIIb-IIIa).

129 The interaction of fibrinogen with integrin alphaIIbbeta3 (GPIIb/IIIa), in part mediated by

130 platelets is initiated by costimulation via integrin alphaIIbbeta3 (GPIIBIIIA)/Galpha13-mediated out

131 Integrin alphaIIbbeta3 has served as an excellent model

132 Structural data of integrin alphaIIbbeta3 have been interpreted as supporti

133 Previous studies of integrin alphaIIbbeta3 have shown that both F2 and F3 bi

134 ty percent of patients with ITP possess anti-integrin alphaIIbbeta3 IgG autoantibodies that cause pla

135 re equivalent in their ability to coactivate integrin alphaIIbbeta3 in a CHO cell system when coexpre

136 he platelet activation marker P-selectin and integrin alphaIIbbeta3 in its active conformation.

137 onstitutively associates with the prototypic integrin alphaIIbbeta3 in platelets and in cell lines ov

138 reading were diminished due to inhibition of integrin alphaIIbbeta3 in platelets from mice expressing

139 ression levels of collagen receptor GPVI and integrin alphaIIbbeta3 in platelets were not affected by

140 here that Src associates constitutively with integrin alphaIIbbeta3 in platelets.

141 platelet function is decreased activation of integrin alphaIIbbeta3 in response to stimulation with a

142 is study, we show that JAM-A associates with integrin alphaIIbbeta3 in resting platelets and dissocia

143 Integrin alphaIIbbeta3 inactivation was unchanged in pla

144 associated regulatory proteins, resulting in integrin alphaIIbbeta3 inactivation, and demonstrate a n

145 3 cytoplasmic domain, coincided closely with integrin alphaIIbbeta3 inactivation.

146 The clinically most widely used integrin alphaIIbbeta3 inhibitor abciximab is a chimeric

147 ir inhibition of platelet aggregation, three integrin alphaIIbbeta3 inhibitors are clinically approve

148 Ex vivo aggregation, secretion, and integrin alphaIIbbeta3 inside-out activation induced by

149 es SERT activity in human platelets and that integrin alphaIIbbeta3 interacts directly with the C ter

150 Integrin alphaIIbbeta3 is a highly abundant heterodimeri

151 messenger-mediated inside-out activation of integrin alphaIIbbeta3 is a key step in platelet aggrega

152 Integrin alphaIIbbeta3 is a member of the integrin famil

153 Integrin alphaIIbbeta3 is a necessary cofactor for PAR1

154 Integrin alphaIIbbeta3 is a predominant type of integrin

155 Aggregation of platelets via activated integrin alphaIIbbeta3 is a prerequisite for thrombus fo

156 The integrin alphaIIbbeta3 is a transmembrane (TM) heterodim

157 -in and inside-out approach at exploring how integrin alphaIIbbeta3 is activated.

158 The platelet integrin alphaIIbbeta3 is critical in hemostasis, and th

159 How integrin alphaIIbbeta3 is discouraged from spontaneous a

160 Platelet integrin alphaIIbbeta3 is maintained in a bent inactive

161 In contrast, outside-in signaling by integrin alphaIIbbeta3 is not altered, but it is inhibit

162 Fibrinogen binding by integrin alphaIIbbeta3 is promoted by platelet agonists

163 Function of the platelet integrin alphaIIbbeta3 is regulated by agonist-generated

164 Integrin alphaIIbbeta3 is widely known to regulate the p

165 xpression of the platelet-adhesion receptor, integrin alphaIIbbeta3, is caused by the presence of reg

166 ation, with particular focus on the platelet integrin alphaIIbbeta3, is provided in this review.

167 The major platelet integrin, alphaIIbbeta3, is required for platelet intera

168 ion of platelet thrombi is determined by the integrin alphaIIbbeta3-mediated interactions of platelet

169 n important role for Rasa3 in PI3K-dependent integrin alphaIIbbeta3-mediated outside-in signaling and

170 was previously identified as an inhibitor of integrin alphaIIbbeta3-mediated outside-in signaling and

171 of talin1 resulted in dramatically impaired integrin alphaIIbbeta3-mediated platelet aggregation and

172 h Ras and Rap1GAP activity and do not affect integrin alphaIIbbeta3-mediated spreading of CHO cells o

173 pressing CHO cells blocked Rap1 activity and integrin alphaIIbbeta3-mediated spreading on fibrinogen.

174 The platelet specific integrin alphaIIbbeta3 mediates platelet adhesion, aggre

175 Fibrinogen binding to integrin alphaIIbbeta3 mediates platelet aggregation and

176 The platelet integrin alphaIIbbeta3 mediates platelet aggregation and

177 Fibrinogen binding to the integrin alphaIIbbeta3 mediates platelet aggregation and

178 The major membrane protein on platelets, integrin alphaIIbbeta3, mediates this response by rapidl

179 For the employed integrin alphaIIbbeta3 model system, the methodology ide

180 Thrombin, Ca(2+), the integrin alphaIIbbeta3, myosin IIa, FXIIIa cross-linking

181 body targeted against active conformation of integrin alphaIIbbeta3 on PMP surface.

182 nds upon the binding of adhesive proteins to integrin alphaIIbbeta3 on the platelet surface.

183 tte encoding human integrin beta3 to restore integrin alphaIIbbeta3 on the surface of megakaryocytes

184 rine (PS) exposure and epitope modulation of integrin alphaIIbbeta3 or a loss of binding of activatio

185 by which platelets regulate the function of integrin alphaIIbbeta3 (or GPIIb/IIIa), the platelet fib

186 ly characterized mouse line with a defect in integrin alphaIIbbeta3 outside-in signaling that affects

187 in in human platelets and a key regulator of integrin alphaIIbbeta3 outside-in signaling.

188 sing the possibility that it participates in integrin alphaIIbbeta3 outside-in signaling.

189 Integrin alphaIIbbeta3 plays a central role in hemostasi

190 Integrin alphaIIbbeta3 plays a critical role in platelet

191 Integrin alphaIIbbeta3 plays a pivotal role in hemostasi

192 According to current dogma, the integrin alphaIIbbeta3 plays an exclusive role in linkin

193 strate that GPIbalpha-mediated activation of integrin alphaIIbbeta3 plays an important role in the fo

194 We present new evidence that the integrin, alphaIIbbeta3, plays a key role in trophoblast

195 where abnormalities of the platelet-specific integrin, alphaIIbbeta3, prevent platelet aggregation fo

196 ructural alteration modifies the function of integrin alphaIIbbeta3, priming the integrin for outside

197 k-2 is tyrosine-phosphorylated downstream of integrin alphaIIbbeta3, raising the possibility that it

198 We analyzed missense variants in the integrin alphaIIbbeta3 receptor subunit genes ITGA2B and

199 Bidirectional signaling of integrin alphaIIbbeta3 requires the beta3 cytoplasmic do

200 ction of the fibrinogen receptor GPIIb/IIIa (integrin alphaIIbbeta3), respectively.

201 Platelet integrin alphaIIbbeta3 responds to intracellular signals

202 K) from the CBD of TS1 activate the platelet integrin alphaIIbbeta3, resulting in platelet spreading

203 which the low-affinity state of the platelet integrin alphaIIbbeta3 results from alphaIIbbeta3 adopti

204 Inactivation of integrin alphaIIbbeta3 reverses platelet aggregate forma

205 nstrates that two orthogonal events regulate integrin alphaIIbbeta3's interactions with fibrinogen, i

206 Outside-in integrin alphaIIbbeta3 signaling is required for normal

207 aling effector downstream of both G12/13 and integrin alphaIIbbeta3 signaling, which contributes to t

208 Binding of platelets to fibrinogen via integrin alphaIIbbeta3 stimulates cytoskeletal reorganiz

209 ts display an approximately 20% reduction in integrin alphaIIbbeta3 surface expression.

210 ete transmembrane and cytoplasmic domains of integrin alphaIIbbeta3, talin1 F2/F3 subdomains, and the

211 hin the extracellular domain of the platelet integrin alphaIIbbeta3 that exhibits many properties tha

212 st beta3 (GPIIIa49-66), a region of platelet integrin alphaIIbbeta3 that is unique.

213 ide LSARLAF, that specifically activates the integrin alphaIIbbeta3 (the fibrinogen receptor), the PD

214 of platelet surface receptors, including the integrin alphaIIbbeta3, the immunoreceptor tyrosine-base

215 s of interactions between fibrinogen and the integrin alphaIIbbeta3, the ligand-receptor pair essenti

216 Integrin alphaIIbbeta3, the most abundant integrin in pl

217 Thus, in the case of one integrin, alphaIIbbeta3, the molecular requirements for

218 latelet binding was promoted by the platelet integrin alphaIIbbeta3: the bacterium bound to purified

219 ent to activate and extend membrane-embedded integrin alphaIIbbeta3, thereby resolving numerous contr

220 g platelets into the thrombus, and activates integrin alphaIIbbeta3 through a pathway that is depende

221 l GPIa/IIa (integrin alpha2beta1) instead of integrin alphaIIbbeta3, thus explaining the clinically m

222 ermal titration calorimetry (ITC) to measure integrin alphaIIbbeta3 TM complex affinity, to study the

223 The structurally unique, highly conserved integrin alphaIIbbeta3 TM complex rationalizes bi-direct

224 l and thermodynamic basis of proline-induced integrin alphaIIbbeta3 TM complex stabilization to under

225 Here, we report the structure of the integrin alphaIIbbeta3 TM complex, structure-based site-

226 he homomeric and heteromeric interactions of integrin alphaIIbbeta3 TM helices in biological membrane

227 The regulated ability of integrin alphaIIbbeta3 to bind fibrinogen plays a crucia

228 The regulated ability of integrin alphaIIbbeta3 to bind fibrinogen plays a crucia

229 smembrane helix of the beta3 subunit enabled integrin alphaIIbbeta3 to constitutively bind soluble fi

230 e platelet surface receptors FcgammaRIIA and integrin alphaIIbbeta3 to induce platelet activation, wh

231 Both functions allow the integrin alphaIIbbeta3 to mediate platelet aggregation.

232 phosphatases are critical for the ability of integrin alphaIIbbeta3 to support stable platelet adhesi

233 n erythroleukemia (HEL) cells, which express integrin alphaIIbbeta3, to investigate whether phosphory

234 e of the WASH complex subunit Strumpellin in integrin alphaIIbbeta3 trafficking in murine platelets.

235 The integrin alphaIIbbeta3 transmembrane (TM) complex is sta

236 Integrin alphaIIbbeta3 undergoes a conformational change

237 dding, exposure of P-selectin, and activated integrin alphaIIbbeta3 upon activation with thrombin wer

238 n the selectivity of these Fab molecules for integrin alphaIIbbeta3 versus the vitronectin receptor a

239 ate and inactivate RhoA, and the function of integrin alphaIIbbeta3 was studied.

240 ansmembrane domains regulate the activity of integrin alphaIIbbeta3, we synthesized a soluble peptide

241 ation of Rap1b and inside-out stimulation of integrin alphaIIbbeta3 were reduced after inhibition of

242 ssing angle between transmembrane helices of integrin alphaIIbbeta3, which eventually results in para

243 signals that drive their activation through integrin alphaIIbbeta3,which serves to prevent inappropr

244 iates the rapid but reversible activation of integrin alphaIIbbeta3, while the adenosine diphosphate

245 at stabilize the bent-closed conformation of integrin alphaIIbbeta3 with alphav integrin binding moti

246 rsubunit disulfide bond, we generated mutant integrin alphaIIbbeta3 with blocked transmembrane separa

247 a4 tail to alphaIIb resulted in a complex of integrin alphaIIbbeta3 with paxillin.

248 The communication of talin-activated integrin alphaIIbbeta3 with the cytoskeleton (integrin o