ライフサイエンスコーパス: interdomain

1 fect the calcium-binding site in the EC4-EC5 interdomain.

2 is predicted to lead to reduced helicity of interdomain A and alter Syk's bias for cis binding.

3 signaling by autophosphorylation on Y130 in interdomain A, since a Y130E phosphomimetic form of Syk

4 cs within the betaSBD play a central role in interdomain allosteric communication in the Hsp70 DnaK.

5 ora B and Map205 in cytokinesis reveals that interdomain allosteric mechanisms can play important rol

6 FVIIa, underscoring a remarkable intra- and interdomain allosteric regulation of this trypsin-like p

7 We find that DNA binding triggers an interdomain allosteric regulation within the GR, leading

8 Understanding the mechanism of interdomain allostery is essential to rational design of

9 nd activity via coevolving residues, whereas interdomain allostery, critical to chaperoning, is robus

10 oreover, mutations in this region compromise interdomain allostery.

11 ide, therefore, an example for destabilizing interdomain allostery.

12 tant in mediating short range and long range interdomain and intersubunit interactions that uniquely

13 2 is facilitated by the unusually wide D1-D2 interdomain angle in OSCAR.

14 in the Sylvatic genotype virus recognized by interdomain antibodies could be the major cause of the p

15 ate, suggesting that they perturb Phi29 DNAP interdomain architecture.

16 strate that perturbing a ctenophore-specific interdomain Arg-Glu salt bridge that is notably absent f

17 als ctenophore-specific features, such as an interdomain Arg-Glu salt bridge, present only in subunit

18 tially populated conformation that adopts an interdomain arrangement is suitable for building a funct

19 imary ligand urokinase and (ii) the flexible interdomain assembly of the three LU domains in uPAR.

20 reased F508del-CFTR steady-state expression, interdomain assembly, and baseline open-channel probabil

21 We observe that transient interdomain association, represented by an effective mol

22 of Zap70 directly bound to PKCtheta, and the interdomain B residues Tyr(315) and Tyr(319) were indire

23 Although mutations in the interdomain-B region affected ITK-SYK kinase activity, t

24 e chains of these residues participate in an interdomain bond, we prepared and examined the functiona

25 the Asp(203)-Arg(678) and Ser(186)-Glu(439) interdomain bonds are critical, because they tighten the

26 EGF2 and EGF3 constitute a rigid rod via an interdomain calcium ion binding site, the long linker be

27 In addition, interdomain cleavage of Dredd is not required for Imd or

28 d II against each other to further close the interdomain cleft between subdomains IB and IIB.

29 tional density modeled as HEPES bound in the interdomain cleft close to the predicted catalytic Lys-1

30 A polymerase (RdRP) domains form a conserved interdomain cleft harboring the coiled-coil domain of hS

31 rylation on two tyrosines located within the interdomain cleft of Galphai.

32 chanisms of action behind secretome-mediated interdomain communication at the gut-mucosal interface.

33 inding underpins a crucial role of Glu340 in interdomain communication between the headpiece and the

34 h may be achieved via linker-domain-mediated interdomain communication driven by ATP hydrolysis.

35 uding protein import, and requires effective interdomain communication for efficient partner-protein

36 ymes, like the PMT, provides new insights on interdomain communication in biosynthetic systems.

37 Thus, eisosome clustering is an example of interdomain communication in response to stress and iden

38 g spines as conduits for effector-dependent, interdomain communication is discussed in light of their

39 to the p97-ND1 interface, thereby modulating interdomain communication of p97 domains and its activit

40 dy, we engineered the transporter to dissect interdomain communication paths.

41 egions within the SBD of mtHsp70s regulating interdomain communication, thus highlighting its importa

42 teractions, bridges an essential pathway for interdomain communication.

43 eotide-binding domain (NBD), thus regulating interdomain communication.

44 P site to the distant GTPase center through interdomain communications and underscore the importance

45 Interdomain communications are likely important for mult

46 Investigation of long-range intra- and interdomain communications in the p53 tetramer-DNA compl

47 The new monomeric interdomain conformation in solution is significantly di

48 ollows a nucleation-propagation process, the interdomain conformational change might be a key step du

49 n with the membrane surface through a large, interdomain conformational change.

50 finity and thermostability by modulating the interdomain conformational dynamics of the antigen-bindi

51 of mutations in the Fab elbow region impacts interdomain conformational flexibility and paratope plas

52 The proposed coupling between intra-/interdomain conformational fluctuations is a consequence

53 for the capsid assembly remains: whether the interdomain conformer within a hexamer unit needs to be

54 ne, which indicates the importance of intact interdomain-connecting elements (i.e. hinge regions) for

55 tallography techniques demonstrated that the interdomain-connecting loop of PCNA interacted directly

56 ngs suggest that unique quaternary folds and interdomain connections in NRs could be exploited by sma

57 ted fatty-acid synthases also extends to the interdomain connections.

58 ture, films with high crystallinity and good interdomain connectivity are obtained.

59 y one order of magnitude for GBs with better interdomain connectivity.

60 have a unique architecture that features an interdomain connector (IDC) that joins the catalytic N-t

61 Proteins make a connection within the interdomain connector loop of PCNA, and much of the regu

62 l C-terminal poly-lysine motif mediates FERM interdomain contacts and assures the tight association w

63 - and beta-domains of HC form several unique interdomain contacts and have a higher shape complementa

64 The PREs show that apo-Pin1 samples interdomain contacts beyond the range suggested by previ

65 due-specific information was obtained on the interdomain contacts in naturally-occurring K48-linked R

66 Alternatively, the interdomain contacts may be sufficient to drive the form

67 sotropic, comprising significantly populated interdomain contacts that appear to be electrostatic in

68 llectively bind to DNA, forming a network of interdomain contacts that links the DNA damage interface

69 ly, such as the requirement of disruption of interdomain contacts to trigger the alpha-to-beta transf

70 ins contain disordered linkers that regulate interdomain contacts, and thus the effective concentrati

71 , and its channel assembly requires multiple interdomain contacts.

72 e activities are repressed by autoinhibitory interdomain contacts.

73 a minimal cell-free system that demonstrates interdomain cooperativity between the ligand (LBD) and D

74 Here, we investigated C2A-C2B interdomain cooperativity with Syt-1 and Syt-7 using dir

75 nced functionalities of full-length TrpRS on interdomain coupling energies between the two new module

76 Moreover, the A-site cAMP, by maintaining interdomain coupling, retards the unbinding of the B-sit

77 agonists and antagonists are anchored in the interdomain crevice of GBR1 by an overlapping set of res

78 ing to wedge in between opposing walls of an interdomain crevice.

79 e L164 and K165 side chains to stabilize the interdomain CRIB:PDZ interface and reposition a conserve

80 , as judged by resistance to proteolysis and interdomain cross-linking.

81 l flexibility of F-actin-binding domains via interdomain cross-talk and consequently inhibiting F-act

82 domain motion than GPA1 and instead displays interdomain displacement resembling that observed in a r

83 On the one hand, the development of the interdomain distance during the unfolding transition sug

84 NADPH and calmodulin are shown to influence interdomain distance relationships as well as reaction c

85 f apo-Pin1 suggests that the fluctuations of interdomain distances are correlated with fluctuations o

86 is necessary to monitor multiple intra- and interdomain distances because a single reporter can lead

87 Using pulsed EPR (PELDOR or DEER) to measure interdomain distances in solution, we have examined two

88 Interdomain distances measured by DEER have been employe

89 The interdomain disulfide bond raises the possibility of oxi

90 However, a unique interdomain disulfide bond was found in the FhGST-S1 whi

91 e cysteine point mutants that engineer extra interdomain disulfide bridges rigidify the UGGT structur

92 cleaved by caspase-3 at a site in the C1-PH interdomain during apoptosis; the functional consequence

93 del system to study the relationship between interdomain dynamics and activity because MMP1 has diver

94 and efficient tool to probe and describe the interdomain dynamics and represents a general method tha

95 However, the correlation between interdomain dynamics and the function of a protein is po

96 However, the roles that interdomain dynamics have in function are not entirely u

97 ed by a multidomain protein showing moderate interdomain dynamics in terms of translational and rotat

98 Activity-dependent interdomain dynamics may enable allosteric control of th

99 We studied the interdomain dynamics of MMP1 on fibrin without crosslink

100 Notably, although a reduction in interdomain dynamics of UbcH5c~Ub is observed upon bindi

101 Therefore, the modulation of interdomain dynamics represents an important mechanism d

102 emonstrate that cpSRP43 exhibits significant interdomain dynamics that are reduced upon binding its S

103 ygenase domains that coordinate a multistep, interdomain electron transfer mechanism to oxidize l-arg

104 ction work also suggested Steap4 utilizes an interdomain flavin-binding site to shuttle electrons bet

105 mulations show that such FWR mutations alter interdomain flexibility in two HIV-1 bnAbs.

106 tures, a 15-A cryo-EM reconstruction reveals interdomain flexibility of the TRXL domains.

107 We characterize the effects of interdomain flexibility on the promotion of DPO4-DNA (un

108 l KaiA pseudoreceiver domains and/or reduced interdomain flexibility.

109 their catalytic rotation is associated with interdomain fluctuations and heterogeneity of conformati

110 y the magnitude and functional dependence of interdomain forces concurrently with the bending elastic

111 ional number of different proteins shaped by interdomain gene transfer.

112 uggest that RssB plasticity, conferred by an interdomain glutamate-rich flexible linker, is critical

113 (+) migration within the polymer blends: (1) interdomain H(+) migration events by "charge-exchange" p

114 substantial fraction of this population was interdomain highly neutralizing flavivirus subgroup-cros

115 lar modeling suggests that alteration in the interdomain hinge angle of KIR2DL3*005 toward that found

116 flexibility for two regions of CK2alpha: the interdomain hinge region, and the glycine-rich loop (p-l

117 in domain, in particular the tendency of the interdomain hinge to buckle, and then we apply an extern

118 e only other approach that focused solely on interdomain hinge-bending regions, the method provides a

119 A series of conserved interdomain His residues is identified to be responsible

120 tion at nine sites, primarily located in the interdomain I-II linker, the region of Nav1.2 crucial fo

121 butes in a global graph, our approach models interdomain information fusion with bipartite graph conv

122 by nSMase2 and is governed by an allosteric interdomain interaction at the membrane interface.

123 These structures illustrate a unique interdomain interaction between the two ATPase domains i

124 The observed interdomain interaction explains PMC's resistance to try

125 first evidence for a direct protein-protein interdomain interaction in La proteins.

126 ction and formation of a predominantly polar interdomain interaction surface.

127 We used protein footprinting to map interdomain interaction surfaces of the sGC signaling do

128 tion between NS3 and NS5 as well as specific interdomain interaction within NS5 required for RNA repl

129 Integrins stabilized the RyR2 interdomain interaction, and this stabilization required

130 gral membrane proteins, such as topology and interdomain interaction, is key to a fundamental underst

131 rtant role for F130, in the stability of the interdomain interaction.

132 ted OCP has an open structure with decreased interdomain interaction.

133 However, it is unclear whether and how the interdomain interactions among the VSD, CTD, and PGD are

134 the TIMP-1 C-terminal domain that stabilize interdomain interactions and improve complementarity to

135 yrin deformation state, but it suggests that interdomain interactions are disrupted by the mutation.

136 hed compartments to establish new intra- and interdomain interactions associated with a B lineage-spe

137 positions 295, 141, and 363 are involved in interdomain interactions at the cytoplasmic side by gove

138 rminant of agonist efficacy, suggesting that interdomain interactions between the ABD and the ATD may

139 Interdomain interactions between the CH3 domains of anti

140 Frustration from strong interdomain interactions can make misfolding a more seve

141 Changes at the interdomain interactions caused either AHR constitutive

142 Recent studies indicate that interdomain interactions critically influence UHRF1's ch

143 Under acidic pH, weakening of the interdomain interactions exposes individual domains, res

144 case for HAI-1, where our results reveal how interdomain interactions have evolved to stimulate the i

145 olyl isomerase (PPIase) domain, resulting in interdomain interactions important for Pin1 function.

146 hich is strongly influenced by the nature of interdomain interactions in resting and active states, m

147 kinase correspond to two dynamic units, but interdomain interactions link the motion of the two doma

148 agenesis and functional assays, we show that interdomain interactions not only stabilize the fold of

149 cture that is stabilized by intersubunit and interdomain interactions of LRRNT and LRRCT in the trime

150 Interdomain interactions of spectrin are critical for ma

151 nhibition of VWF mediated by force-dependent interdomain interactions offers the molecular basis for

152 The effect of the interdomain interactions on the activation of motions in

153 introduce bulky residues into tight Gly-Gly interdomain interactions on the periplasmic side of LacY

154 Together, our data reveal interdomain interactions responsible for communicating N

155 ain proteins that lack these specific strong interdomain interactions should fold reliably.

156 r acts as a conformational switch leading to interdomain interactions that are critical to enzyme act

157 E2A or PU.1 were associated with intra- and interdomain interactions that are developmentally regula

158 ified oligonucleotides evolved by optimizing interdomain interactions that stabilize the catalyticall

159 G168D mutation was associated with weakened interdomain interactions under tensile force.

160 Accordingly, we have investigated Pin1 interdomain interactions using NMR paramagnetic relaxati

161 Transient interdomain interactions via regions crucial for Hsp70 b

162 Upon light stimulation, interdomain interactions weaken to facilitate activation

163 Previous work showed that interdomain interactions within nSMase2 are needed for P

164 says, to define the mechanism mediating this interdomain interactions within nSMase2.

165 athways, emanating from hydrophobic or polar interdomain interactions, differentially affecting lipid

166 decouples control over the domain shape and interdomain interactions, leading to a multiplicity of p

167 anisms of multidomain proteins often exploit interdomain interactions, or "cross-talk." An example is

168 ensive heterodimerization interfaces and AHR interdomain interactions.

169 affected by the dimerization interfaces and interdomain interactions.

170 amily but also how the two domains engage in interdomain interactions.

171 und that charges contain the information for interdomain interactions.

172 intradomain interactions but also increased interdomain interactions.

173 ion changes its conformation and/or mediates interdomain interactions.

174 lding, and cooperative folding, modulated by interdomain interactions.

175 otein concentration, and the strength of the interdomain interactions.

176 ivated FVIII (dis-FVIIIa), may contribute to interdomain interactions.

177 erolemia (FH) and clustered at the predicted interdomain interface (R469W, R496W, and F515L) inhibite

178 Thus, the structure and stability of this interdomain interface are crucial for the role of sigma(

179 lding region (residues 824-858) at the spike-interdomain interface displayed dramatic structural rear

180 nker is a well-structured participant in the interdomain interface in ATP-bound Hsp70s.

181 olding nucleus is disrupted by mutation, the interdomain interface is sufficiently stable to drive th

182 by acting as molecular wedges that restrict interdomain interface movement behind the selectivity fi

183 ion network connecting the active site to an interdomain interface responsible for nucleotide loading

184 coupled with the remarkable stability of the interdomain interface result in cooperative folding kine

185 istant conformation maintained by a critical interdomain interface within a negative regulatory regio

186 igma(N)-holoenzyme model reveals a conserved interdomain interface within sigma(N) that, when disrupt

187 model given the increased flexibility at the interdomain interface, and we can for the first time exp

188 y conserved Ile and Phe residues at the RfaH interdomain interface.

189 se inhibitor (APHI) that binds a site in the interdomain interface.

190 reduction in the buried surface area at the interdomain interface.

191 binding pocket at one pole and an equatorial interdomain interface.

192 form a rigid interaction through a conserved interdomain interface.

193 the domain structures and properties of the interdomain interfaces indicate that interconversion bet

194 Ca(2+), ATP, and caffeine were identified at interdomain interfaces of the C-terminal domain.

195 mutations establishes a correlation between interdomain interfaces of the enzyme and missense varian

196 Pervasive H-bonding is found at all interdomain interfaces, which may contribute to their ma

197 by an unstructured approximately 80-residue interdomain linker (IDL).

198 Here we find that the interdomain linker also affects the single-base deletion

199 which the Ras-binding site is blocked by an interdomain linker and the membrane-interaction surface

200 This preferential binding implicates the interdomain linker as a dynamic allosteric switch.

201 tify a tetrapeptide motif (RXWV) in the TlyA interdomain linker as indispensable for co-substrate bin

202 The interdomain linker becomes statically disordered.

203 lammatory caspases cleave gasdermin-D in the interdomain linker but not GSDMB.

204 ts an unexpected locus for the binding of an interdomain linker element in DnaI/DnaC-family proteins.

205 a-helical lid, and the conserved hydrophobic interdomain linker enable allosteric signal transmission

206 interface is created by the insertion of an interdomain linker from C6 into a hydrophobic groove cre

207 ining the PH domain and a 17-amino acid-long interdomain linker immediately N-terminal to the first b

208 explore the conformational landscape of the interdomain linker in ADP-bound DnaK and supported our s

209 es, we have previously demonstrated that the interdomain linker is a major determinant of polymerase

210 The interdomain linker is a well-structured participant in t

211 fied by the Escherichia coli Hsp70 DnaK, the interdomain linker is flexible.

212 Finally, we investigate the relation between interdomain linker length and misfolding, and propose a

213 ore, we provide evidence that the disordered interdomain linker modulates the histone-binding affinit

214 Thus, the PH domain and the interdomain linker of Brag2 may be targets for selective

215 o consist of adjacent protomers engaging the interdomain linker of one molecule in the substrate bind

216 gma(K) depends on particular residues in the interdomain linker of SpoIVFB.

217 o alters the packing of the highly conserved interdomain linker of the PER2 PAS core such that, altho

218 Our data provide an example of how interdomain linker plasticity can modulate the function

219 ) a four-Gly (FNIII9(4G)10) insertion in the interdomain linker region and used surface plasmon reson

220 The HD oligomerization is driven by an interdomain linker region that acts as a latch to 'catch

221 We thus identify 10 residues in the interdomain linker region that change their conformation

222 on of histone binding by a histone-mimicking interdomain linker represents another example of regulat

223 We found that while the interdomain linker samples many conformations, it behave

224 Hsp70 interdomain linker sequences are highly conserved; moreo

225 ADP-bound conformation and clashing with the interdomain linker that occupies this site in ATP-bound

226 ormational activation of AP-2 by the Fcho1/2 interdomain linker to promote AP-2 cargo engagement.

227 teraction motif between W77 of S3, the S4-S5 interdomain linker, and the C-terminus, which is associa

228 d eIF4B/-3 binding site within the HEAT-1/-2 interdomain linker, harboring two phosphorylation sites

229 Thus, a small region of the interdomain linker, located more than 11 A away from the

230 and RRM1 as well as the participation of the interdomain linker, probably in realizing tandem domain

231 atricopeptide repeat 1 (TPR1) domain and the interdomain linker, respectively.

232 that the conformational fluctuations of the interdomain linker, which are largely responsible for th

233 tone reader module, including its 20-residue interdomain linker.

234 at binding by beta depends critically on the interdomain linker.

235 e PH domain and that activity depends on the interdomain linker.

236 rees ) at the end of the relatively immobile interdomain linker.

237 nding domain (SBD), which are tethered by an interdomain linker.

238 The role of interdomain linkers in modular polyketide synthases is p

239 Structural modulation of interdomain linkers may thus constitute a general strate

240 Our findings further support the concept of interdomain linkers serving a dual role in substrate bin

241 o by various non-structured regions, such as interdomain linkers, or terminal sequences.

242 an additional DNA-binding domain and longer interdomain linkers, the architecture of a canonical thr

243 eported all beta-fold, flanked by disordered interdomain linkers.

244 riety of interdomain orientations due to two interdomain linkers.

245 Using the interdomain loop conformation as the reaction coordinate

246 and Arg-103, residues in the vicinity of the interdomain Lys-99-Asp-101 salt bridge, have little or n

247 hought to fold cotranslationally to minimize interdomain misfolding.

248 rotein is further slowed by the formation of interdomain misfolds, suggesting that with growing chain

249 The interdomain motion turns out to be limited.

250 x reveal that it is dynamic, with persistent interdomain motions enabling the linker and N3 domains i

251 Challenges encountered in deciphering interdomain motions for this ternary complex are discuss

252 Here, we characterize the interdomain motions in the calcium-bound state of calmod

253 the computational prediction of large-scale interdomain motions is not trivial and may lead to erron

254 ulations allowed for the characterization of interdomain motions of a compact state of PGK.

255 hich are largely responsible for the overall interdomain motions of CaM, can be well described by exp

256 lations have captured functionally important interdomain motions of NS3 helicase and reproduced singl

257 led because mutations were incompatible with interdomain motions required for catalysis.

258 a high flexibility of Scl2.3 with remarkable interdomain motions that are likely instrumental to the

259 lly harbor a buried ligand-binding pocket at interdomain or intersubunit clefts, facilitating proper

260 d consistently located within or near to the interdomain or solvent-exposed regions in the antibody s

261 The relationship between interdomain order disparity and the stability of phase s

262 that this binding mode likely influences the interdomain orientation.

263 l fold, these domains exhibited a variety of interdomain orientations due to two interdomain linkers.

264 Ca(v)1.2 (IQ), which adopts three different interdomain orientations in the crystal.

265 ordered, cholesterol-poor/PSM-rich/DOPC-rich interdomain phase.

266 fate and p-cresol sulfate dock on a putative interdomain pocket of the extracellular EGF receptor.

267 1*005 correlated with an altered KIR3DL1*005 interdomain positioning and increased mobility within it

268 eric bispecific antibodies by exchanging the interdomain protein interface of the human IgG1 CH3 dime

269 dergoes one of the largest substrate-induced interdomain rearrangements documented to date.

270 osomal translocation is accompanied by large interdomain rearrangements of elongation factor G (EF-G)

271 mediated by different types of cysteine-rich interdomain region (CIDR) domains found in the N-termina

272 n be classified based on their cysteine-rich interdomain region (CIDR) domains.

273 d to brain endothelium and the cysteine-rich interdomain region 1 inhibited binding of P. falciparum-

274 68D replacement along the AD facing the CfaE interdomain region was previously shown to decrease the

275 within the micrometer-scale domains and the interdomain region.

276 crease the positive charge at the rim of the interdomain region.

277 ree compositionally distinct phases: (1) the interdomain region; (2) micrometer-scale domains (d > 3

278 ciparum virulence factors: two cysteine-rich interdomain regions (CIDR) alpha1 (IT4var19 and PFCLINva

279 ar ectodomains made from CIDR (cysteine-rich interdomain regions) and DBL (Duffy-binding-like) domain

280 Our findings reveal exquisitely precise interdomain regulation within IRE1, advancing the mechan

281 Aiming to uncover interdomain regulatory mechanisms in SHIP2, we determine

282 hosphorylation of Asp(60) of AccR alleviates interdomain repression mediated by the N-terminal domain

283 MrkH structures reveals a large 138 degrees interdomain rotation that is induced by binding an inter

284 he separation between domains increased, and interdomain rotations became more frequent.

285 ecular dynamics simulations suggest that the interdomain salt bridge acts as a steric barrier regulat

286 dynamics simulations identified three novel interdomain salt bridges in the lymphomagenic virus HR1

287 2 further suggest important contributions of interdomain salt-bridge interactions to the stabilizatio

288 s and are closed at the matrix side by three interdomain salt-bridge interactions, one of which is br

289 hypothesize conformational plasticity in an interdomain segment of FliG that allows some subunits to

290 nding to the WW domain simultaneously alters interdomain separation and the internal conformation of

291 Our results suggest that an interdomain separation facilitates opening up the cataly

292 s, so as to establish the dynamical basis of interdomain signal transduction in Hsp70s.

293 A primary reason why study of interdomain signaling is challenging in oligomeric prote

294 We characterize here interdomain site-to-site communication by which a common

295 Recently, a Ca(2+)-driven interdomain switch has been described, albeit how it cou

296 The latter structural elements make interdomain tertiary contacts (L5/P3) that span a region

297 nd C-terminal domains, including an extended interdomain tunnel associated with substrate channeling.

298 which is dependent on temperature-sensitive interdomain unfolding and cis-trans prolyl isomerization

299 odine receptor 2 [RyR2]) in heart failure is interdomain unzipping that can enhance aberrant channel

300 Thus, the interdomain/WW domain conformations sampled by apo-Pin1