ライフサイエンスコーパス: internal standard

1 f several concatenated signature peptides as internal standard.

2 study, is corrected by referencing versus an internal standard.

3 dard, and a spiked lanthanide as a secondary internal standard.

4 d by normalizing signal intensities using an internal standard.

5 17beta-estradiol-D5 was used as internal standard.

6 ilution using mass-labeled 1,4-dioxane-d8 as internal standard.

7 Dihydroisosteviol was used as an internal standard.

8 ion relies on the ratio of the target to the internal standard.

9 iment using isotope labeled amitriptyline as internal standard.

10 known concentration of propranolol-d7 as an internal standard.

11 mode with deuterium-labeled vitamin K1 as an internal standard.

12 e calibration curves and sulfanilamide as an internal standard.

13 Monitoring mode using labeled glutathione as internal standard.

14 ith respect to thorium, considering it as an internal standard.

15 peptide using stable isotope labeled peptide internal standard.

16 ) in the positive ion mode with DMA D6 as an internal standard.

17 sera, using a non-human cel-miR-39-3p as an internal standard.

18 e introduced as a conceptually new source of internal standard.

19 shed along with the acceptable range for the internal standard.

20 using curcumin as the non-isotopic isobaric internal standard.

21 S/MS quantification using genistein-d4 as an internal standard.

22 achieved while using an isotopically labeled internal standard.

23 The quantification of the PAHs utilised an internal standard.

24 4 min using positive APCI ionisation and an internal standard.

25 rium labelled d(3)-acrylamide was used as an internal standard.

26 ate, using green fluorescence protein as the internal standard.

27 alkylated rather than intact hepcidin as the internal standard.

28 t, such as Cr(acac)3, and CH3(13)COONa as an internal standard.

29 evaluate the normalization potential of the internal standards.

30 corresponding deuterium-labeled analogues as internal standards.

31 re affected by the choice of calibration and internal standards.

32 using deuterated analogues of these drugs as internal standards.

33 ded on mass spectrometry and isotope-labeled internal standards.

34 of traditionally used synthetic peptides as internal standards.

35 m mass spectrometry (LC-MS/MS) together with internal standards.

36 conducts harmonization according to its own internal standards.

37 raditional methods of standard additions and internal standards.

38 olutions of D4/D5 and (13)C-labeled D4/D5 as internal standards.

39 PCB 155 and PCB 198 were employed as internal standards.

40 each barrel was continuously monitored with internal standards.

41 ated propane and butane generated in situ as internal standards.

42 is not possible using the traditional acidic internal standards.

43 ain no polymorphisms, so they can be used as internal standards.

44 dimensional NMR and mass spectrometry, using internal standards.

45 und for each studied family of beef, and two internal standards.

46 stable isotopes ((13)C and (15)N) for use as internal standards.

47 ent settings, collision energy, and employed internal standards.

48 er was achieved using stable-isotope-labeled internal standards.

49 51 of 55 samples were spiked with 19 labeled internal standards.

50 hased commercially or synthesised for use as internal standards.

51 ssues, and quantified relative to deuterated internal standards.

52 biomass and used them as tracers ((15)N) and internal standards ((13)C).

53 0.98 over about 3 orders of magnitude if an internal standard (15:0 FFA) was added.

54 eum biomarkers, notably including the native internal standard 17alpha(H),21beta(H)-hopane, commonly

55 (24R),25(OH)2D3 and added deuterated labeled internal standard (24R),25(OH)2D3-d6 were extracted from

56 When using green fluorescent protein as an internal standard, 77K fluorescence emission spectra on

57 l in pooled human plasma with ramipril as an internal standard, a greater than 4-fold improvement in

58 iplex Absolute Regressed Quantification with Internal Standards-a novel mass spectrometry-based appro

59 ll resolution by normalizing to a DGTS(32:0) internal standard added to the LVC probe solvent during

60 Quality controls based on the results of internal standards added at the samples' extraction, dil

61 Stable isotope labeled (SIL) internal standards allow for matrix-independent quantita

62 nstrated that PRM with SILAC histones as the internal standards allowed reproducible measurements of

63 Sin a 1, used as an internal standard, allowed quantification of this mustar

64 he analysis was investigated: sample amount, internal standard amount, and sample dilution factor.

65 inal optical (LO) phonon, which serves as an internal standard and confirms the sensitivity of the te

66 y isotope of 2'-deoxycytidine was used as an internal standard and one-step multi-enzyme digestion wa

67 vour dilution (FD) factor were quantified by internal standard and relative response factor.

68 that both the lipid class and adduct of the internal standard and target analyte match, while a rank

69 n-Propanol was used as an internal standard and the three carboxylate peaks were r

70 and highlights the need for use of suitable internal standards and isotope dilution techniques.

71 es and is only quantitative when compared to internal standards and reference cell lines.

72 mogenized with isotopically labeled analogue internal standards and sampled using gas-phase SPME.

73 by tandem mass spectrometry using synthetic internal standards and selected reaction monitoring.

74 n were achieved using stable isotope-labeled internal standards and tandem mass spectrometry.

75 n of 4-6%, stable migration times (1.4% with internal standard), and capillary recoveries on the orde

76 DHB as MALDI matrix, a primary lipids-based internal standard, and a spiked lanthanide as a secondar

77 The Au core of the Au@Ag NPs acts as an internal standard, and aids in the analysis of the compl

78 served peptides, a heavy isotope labeled mAb internal standard, and anti-human Fc enrichment.

79 n D(2)O as the solvent, sodium acetate as an internal standard, and heating at 90 degrees C for 1 h.

80 ine adduct, addition of isotopically labeled internal standard, and quantitation by LC-MS/MS analysis

81 f the fragment ions from the analyte and its internal standard, and the ratio is used in quantitative

82 NIST SRM 987 Sr was used as an internal standard, and the reproducibility of the result

83 astereomeric mixture of isotopically labeled internal standards, and multiple reaction monitoring mas

84 Normalization to the internal standard appeared most effective when the inten

85 nsured the reproducibility of the matrix and internal standards application with RSD of 10-15%.

86 s for using isotopically labeled rhGH as the internal standard are described.

87 n each laser shot, even when the analyte and internal standard are quite disparate in m/z, thereby im

88 In the present work, isotopically labeled internal standards are applied to tissue sections to max

89 ty is introduced into SVOC quantitation when internal standards are not added promptly into SVOCs sol

90 If multiple internal standards are provided for a lipid class, stand

91 tical standards, including deuterium-labeled internal standards, are also described.

92 normalization techniques, mainly focusing on internal standard-based approaches, has been performed t

93 trapolation to the nominal temperature, (vi) internal standard-based correction, and (vii) simple rec

94 mogenization is traced by the addition of an internal standard before storage.

95 To address these problems, we developed Internal Standard Calibrated ChIP (ICeChIP), wherein a n

96 The method performance evaluated with internal standard calibration method produced good analy

97 We demonstrate for the first time that this 'internal standard' calibration method satisfies the sine

98 Single-point internal standard calibrations were used to estimate abs

99 oximately 1 muM, and calibration curves with internal standards can achieve a precision of 1-9% withi

100 The internal standard capillary electrophoresis method (IS-C

101 ; AOAC, 991.41, 1995; AOAC 998.12, 2005) and Internal Standard Carbon Isotope Ratio Analysis could no

102 hat multiple reaction monitoring assay using internal standards carrying peptides for quantification

103 lso allows for testing of different analytes/internal standard combinations, which helps with the sel

104 Seven were quantified using an internal standard combined with external calibration cur

105 nation of internal correction using Ni as an internal standard (coming from a conebulized standard so

106 To test how well the internal standards compensate for differences in tissue

107 atio on the y-axis versus the inverse of the internal standard concentration on the x-axis.

108 type (H(+), Na(+), and K(+)) for analyte and internal standard corrected for different ionization eff

109 Normalization to internal standards corrected a 2-fold difference in lipi

110 , were 91-121% and 90-113% (without and with internal standard correction, respectively), for wines f

111 tandard deviation (%RSD) of 7.7/7.5% without internal standard correction.

112 The Se(13)C(15)N(-) internal standard could be used for isotope dilution.

113 The internal standard curcumin yielded good linearity (R(2)

114 onventional relative quantification using an internal standard curve and need for calibrant diluent,

115 gs to enable simultaneous construction of an internal standard curve in the MS(1) precursor scan, rea

116 avy-labelled standard peptides, to construct internal standard curves for peptides derived from key n

117 Internal standards DA-d4 and DOPAC-d5 result in standard

118 17 or -1.08 V versus a ferrocene/ferrocenium internal standard depending on the type of functionaliza

119 lly, quantification via structurally similar internal standards, different isotope labeling strategie

120 euterated derivative 1b is of interest as an internal standard during MC quantification in biological

121 hich are subsequently utilized as endogenous internal standards during integration.

122 led using biologically derived (13)C labeled internal standards eliminating systematic errors related

123 ase extractions and by the use of MK-4 as an internal standard enabled the quantitation of microgram

124 This internal standard enables to map the detected intensity

125 rds [(15)N5]dG-gx-dC and [(15)N5]dG-gx-dA as internal standards, enzyme hydrolysis to release the cro

126 Prior to optimization, internal standards exhibited strong matrix effects with

127 When pH-insensitive AuNCs were used as an internal standard, FITC/BSA-AuNCs offered a sensitive an

128 uterated tricin analog was synthesized as an internal standard for accurate quantitation purposes.

129 over 3 days while using a single nonisotope internal standard for data normalization.

130 VSKKDSGASP(R) (without thioether bridges) as internal standard for determination of nisin A in cream

131 sed method can be utilized to generate novel internal standard for isotope dilution and to extend the

132 methods of applying an isotopically labeled internal standard for MALDI IMS analysis.

133 at deuterium labelled MK-7 can be used as an internal standard for MK-9.

134 partially loaded with an easily synthesized internal standard for MS quantitation.

135 e route, providing access to 0.110 g of this internal standard for use in the detection and precise q

136 omparable and (2) nine glycolytic enzymes as internal standards for a more accurate calculation of re

137 r, isotopic labeled substances are needed as internal standards for a reliable and convenient quantif

138 rement and the use of stable isotope-labeled internal standards for absolute quantitation.

139 Fully (13)C-labeled sugars are used as internal standards for composition analysis.

140 of lipid species in biological tissues using internal standards for each lipid class.

141 ction linked to the recovery of mass-labeled internal standards for every analyte within every sample

142 In this work, we synthesized internal standards for four garlic organosulfur compound

143 ) as a derivatizing agent and polydeuterated internal standards for maximum accuracy and precision.

144 lipid classes, were spiked to each sample as internal standards for postacquisition normalization.

145 that employed stable isotope-labeled peptide internal standards for quantitation of HMPV.

146 wave-assisted deuterium exchange, for use as internal standards for stable isotope dilution analysis.

147 Internal standards for the absolute quantification of th

148 ration, thus facilitating the preparation of internal standards for the accurate quantification of en

149 using isotopically labeled (IL) analogues as internal standards for the target analytes are required

150 Transferrin levels obtained by both internal standards give overlapping, or nearly overlappi

151 The utilization of (13)C internal standards greatly improved the accuracy and rel

152 plied to vanillin with dimethylsulfone as an internal standard, has an equivalent accuracy (<1 per th

153 Internal standards have been introduced in quantitative

154 t using stable isotope labeled (SIL)-protein internal standard (I.S.).

155 approaches utilizing various calibrators and internal standards (I.S.) have been developed.

156 characteristics were evaluated for different internal standards, i.e., PEGylated and polyhistidine-ta

157 eference of analyte intensity to that of the internal standard in each laser shot, even when the anal

158 peak area ratios of the analytes versus the internal standard in five repetitive analyses over appro

159 oalanine and (13)C labeled BCA is used as an internal standard in gammaGBCA analysis.

160 he limit of detection (LOD) for the isobaric internal standard in linear regression.

161 mall volumes of sample, where addition of an internal standard in the sample is either troublesome or

162 molecule propranolol and its isotope labeled internal standard in water, unprocessed river water and

163 tification of the metabolites using isotopic internal standards in the exploratory cohort as well as

164 ries and provide an example for their use as internal standards in the profiling and absolute quantif

165 eference procedure were: (1) use of EC-d5 as internal standard instead of less similar propyl carbama

166 By spiking an isotopically labeled internal standard into the plasma sample, a linear calib

167 gnetic resonance (qHNMR) with an appropriate internal standard is a well-established quantitation met

168 hoice of appropriate stable isotope-labelled internal standard is crucial, given the diversity of enc

169 ter areas of homogenized tissue, provided an internal standard is sprayed on to the tissue prior to a

170 marker analysis provided that an appropriate internal standard is used and that readouts are monitore

171 )C NMR, which can even be misleading when no internal standard is used as in the above-mentioned pape

172 quantitative method employing ethanol as an internal standard (IS) for GC-MS quantification of volat

173 t time, a polymeric (13)C lignin was used as internal standard (IS) for lignin quantification via ana

174 For targeted analysis, both an internal standard (IS) method, as well as the ERETIC2 to

175 endent experiments because the (13)C-labeled internal standard (IS) used to determine the concentrati

176 acids (FAAs) in rice using l-theanine as the internal standard (IS) with o-phthalaldehyde (OPA) reage

177 concerning the extraction process, usage of internal standard (IS), and influences on the ionization

178 )) in human plasma using dapagliflozin as an internal standard (IS).

179 ction models of SSB alone, SSB combined with internal standard (IS-SSB), and ORM-SSB were compared fo

180 ficult to control, are improved by utilizing internal standards (IS).

181 In this study, we fabricated standards and internal standards (ISs) for absolute quantification via

182 ive LC-MS/MS typically relies on addition of internal standards (ISs).

183 olled and operated the ITEX preconditioning, internal standard (ISTD) addition, air sampling, and ITE

184 This work proposes to integrate an internal standard (ISTD) into the microchip by adding it

185 using [(13)C2, (2)H3]-labeled isotopologs as internal standards; (iv) sequencing paCOS by tandem MS.

186 ples are not completely represented in these internal standards, leading to orphan analytes for which

187 and reproducible measurements with a droplet internal standard (<10% RSD) and compare the sensitivity

188 aches using a matrix-adapted calibration, an internal standard material and a modified standard addit

189 e also showed that the quantification by the internal standard method can be done on the low-field NM

190 This internal standard method proved to be more accurate than

191 and GCMS analyses as well as quantitation by internal standard method showed that (Z)-3-hexenal, ethy

192 gn and implementation of a coinjected triple-internal standard method to alleviate the need of an acc

193 Quantification was performed by the internal standard method using a five points regression

194 Isotope-labeled internal standard method was used for the quantitation.

195 the response factors approach along with the internal standard method, allowing for quantification of

196 xternal calibration, standard additions, and internal standard methods using ICP OES.

197 An isotopically labeled internal standard mix was incorporated into the extracti

198 A single-step metabolite extraction with internal standard mixture (in methanol) warranted high-t

199 aser energy and varied the amounts of matrix-internal standards mixture to evaluate the normalization

200 Internal standard-normalized data allowed intrasample mo

201 one point gravimetric standard addition with internal standard of the same (29)Si spike was employed

202 derate matrix content, the use of an optimum internal standard (OIS) at a concentration close to that

203 at combined signal correction via preloading internal standard onto SPME fibers and signal integratio

204 have previously shown that the deposition of internal standards onto the tissue section during sample

205 ers, including comigration with a deuterated internal standard or in silico prediction of electromigr

206 synthetic stable isotope-labeled peptides as internal standards or surrogate standards.

207 concentration without the use of calibrants, internal standards, or a calibration curve.

208 antitation that does not rely on calibrants, internal standards, or calibration curves but, rather, l

209 pport the use of recombinant SIL proteins as internal standards over SIL peptides due to their abilit

210 Isotopically labeled internal standards, Paraquat-D6 and Diquat-D4, were used

211 present study proposed a postcolumn infused-internal standard (PCI-IS) assisted strategy to improve

212 The postcolumn infused internal standard (PCI-IS) method was applied to estimat

213 a novel strategy using a postcolumn infused-internal standard (PCI-IS) method with liquid chromatogr

214 onization using postcolumn infusion of eight internal standards (PCI-IS).

215 g/mL) generated by dividing MMC peak area by internal standard peak area and plotting the area ratio

216 erformed with peak areas, ratios of standard/internal standard peak area, and concentrations.

217 also have implications for the selection of internal standards, peaks used for internal mass calibra

218 ymorphism-containing peptides to appropriate internal standard peptides were linear and had excellent

219 Moreover, the use of isotope labeled internal standards permitted the quantification of the c

220 We evaluated three conventional internal standard platforms (synthetic peptides, QconCAT

221 ed to assess quantitative performance of the internal standard platforms.

222 Instead of relying on an internal standard polymer, the intensities of 40 poly(8:

223 is determined relative to a perchlorate-ion internal standard, preloaded into the vesicle along with

224 This material, added as an internal standard prior to trypsin digestion, produces a

225 A fit-for-purpose method using internal standard procedure for accurate quantitation of

226 spectrometry, utilizing a specially designed internal standard procedure.

227 Comparing fingerprinting with and without internal standard proved that the presence of the (13)C

228 Internal standard provided significant improvement (p <

229 tive MS instrument with field spiking of the internal standard provides accurate phosphate results an

230 atched calibration method using carvacrol as internal standard, providing quantification limits betwe

231 The addition of internal standard reduced the coefficient of variation f

232 user can also signify which lipid classes an internal standard represents, for example indicating tha

233 cts, good RRs are obtained regardless of the internal standard selected.

234 are used to prepare a plot of the analyte-to-internal standard signal ratio on the y-axis versus the

235 e results showed, even after correction with internal standard, significantly lower intensities from

236 with known amounts of stable-isotope-labeled internal standards (SIL-IS) allows measurements of the c

237 l ions were redispersed in a small volume of internal standard solution and deposited onto a quartz r

238 addition of (34)S and a generic S-containing internal standard spiked to the sample provides full com

239 metabolomic experiment; and (2) mixtures of internal standards spiked in plasma samples at varying c

240 icle describes the evaluation of a practical internal standard spray addition technique, used prior t

241 Separation resolution between the internal standard, substrate, and product is 1.2 and ave

242 is mainly due to the limited availability of internal standards that can adequately correct for varia

243 Using bis(2-ethylhexyl) sebacate as an internal standard, the kinetics of the ozone-BeP reactio

244 ncluding the amount of peptide substrate and internal standard, the timing of cleavage reaction, and

245 derivatization and a stable isotope labeled internal standard, the total retronecine-/heliotridine-t

246 t prior consideration of R TS and the use of internal standards, the relative increase in GFP calcula

247 spectroscopy, with and without the use of an internal standard (TiO(2)).

248 The phosphate backbone signal was used as an internal standard to calibrate the absolute signal of ea

249 which employs metal-encoded microbeads as an internal standard to correct the data for instrumental s

250 Dantron was used as an internal standard to ensure accuracy and reproducibility

251 otope-labeled full-length standard CD4 as an internal standard to measure endogenous CD4 directly, wi

252 The use of a internal standard to normalize regional differences in i

253 euterium-labeled cholesterol was used as the internal standard to obtain the absolute quantity of the

254 species, researchers often apply individual internal standards to a broad range of related lipids.

255 thod based on application of matrix and dual internal standards to allow intra- and intersample norma

256 ll-E-beta-apo-8'-carotenoic acid ethyl ester internal standards to compensate for analytical variatio

257 eparate the three metabolites, (ii) isotopic internal standards to correct for in-source pGlu formati

258 ated/ammoniated adduct ion ratios of applied internal standards to correct for these interferences.

259 enone, d3-skatole and d6-indole were used as internal standards to eliminate matrix effects.

260 -labeled analogues were prepared and used as internal standards to identify and quantify these charac

261 Without internal standards to match each lipid species, research

262 t protein and employ isotope-labeled peptide internal standards to quantify chosen surrogate peptides

263 The quest for internal standards useful in MALDI imaging studies goes

264 lozapine, and buspirone and their deuterated internal standards using the matrix 3-nitrobenzonitrile

265 e when the intensity ratio of the analyte to internal standard was approximately one, and thus preclu

266 uantification method using ((2)H2)GABA as an internal standard was developed and validated according

267 on the dilution series, where the use of the internal standard was necessary to achieve accuracy and

268 nnose, fructose and ribose) plus inositol as internal standard was obtained in 500mmol.L(-1) triethyl

269 meant that quantitative analysis without an internal standard was possible.

270 on assay (SIDA) with d7-gamma-decalactone as internal standard was used for quantitative analysis of

271 able isotope dilution using creatinine-d3 as internal standard was used for quantitative measurements

272 In this study, a deuterated internal standard was used to account for these signal v

273 A fully carbon-13-labelled internal standard was used to compensate for matrix effe

274 Internal standard was used to validate the efficiency of

275 AI determined for five consecutive days with internal standards was better than 20% using manual samp

276 luated and a standard addition protocol with internal standards was chosen for analyte quantitation.

277 ors and stable isotope labeled (SIL) peptide internal standards was validated for human liver.

278 rfaces by DBDI-MS, using TATP-3,6,9-(13)C as internal standard, was done and validated using an LC/AP

279 By using an internal standard, we are able to quantify the density o

280 By leveraging water as an internal standard, we quantify MCHC of healthy RBCs and

281 Using DART-MS/MS and isotopically labeled internal standards, we achieved detection limits of 21 n

282 Using deuterated homologues as internal standards, we observed limited and relatively s

283 Using a preformulated mixture of eight internal standards, we quantified the glycans in a monoc

284 ds for applying calibration standards and an internal standard were evaluated.

285 The analytes and internal standard were extracted from human plasma using

286 No sample preparation or internal standards were needed for calibration.

287 rominated standards and isotopically labeled internal standards were synthesized and purified to conf

288 Internal standards were synthesized for internal absolut

289 ic reference standards and deuterium-labeled internal standards were synthesized for use in an assay

290 Five internal standards were tested, with potassium hydrogen

291 Isotope-labeled internal standards were used for all analytes.

292 While only two internal standards were used for correction, one per ion

293 including a [(13)C]-labeled version used as internal standard, were immuno-enriched from rat serum w

294 m the shell, and a probe molecule as a Raman internal standard, were rationally designed for quantita

295 ibrators) and external calibration (i.e., as internal standards) when quantifying three forms of unla

296 etabolite standards and isotopically labeled internal standards, whereas the corresponding mitochondr

297 analysis with fully (13)C-labeled biomass as internal standard, which is generated by growing E. coli

298 g/mL was achieved by employing (15)N-IGF1 as internal standard, which proved to be an essential featu

299 The gQD/Cy3 FRET pair served as an internal standard, while the rQD/A647 FRET pair served a

300 a method combining synthetic RNA-DNA-hybrid internal standards with high-resolution, strand-specific