ライフサイエンスコーパス: intrinsic fluorescence

1 or NAD+ (detected via changes in the protein intrinsic fluorescence).

2 canning calorimetry, circular dichroism, and intrinsic fluorescence.

3 scanning calorimetry, circular dichroism, or intrinsic fluorescence.

4 was resolved by stopped-flow measurements of intrinsic fluorescence.

5 s (K(d) = 11-12 microm) and changes in FV(a) intrinsic fluorescence.

6 s C by following a decrease in the protein's intrinsic fluorescence.

7 ide at this site would result in a change in intrinsic fluorescence.

8 dent denaturation and by iodide quenching of intrinsic fluorescence.

9 s were replaced by Phe residues, eliminating intrinsic fluorescence.

10 ha subunit conformational change detected by intrinsic fluorescence.

11 red mammalian-cell-expressed TIMP-(1-127) by intrinsic fluorescence.

12 ged), to study the possible changes of their intrinsic fluorescence.

13 uman plasma were characterized by changes in intrinsic fluorescence.

14 hanges in HSA, evidenced by a reduced enzyme intrinsic fluorescence.

15 ctra showed that all ligands quenched enzyme intrinsic fluorescence.

16 m, the visual cycle, and other roles exhibit intrinsic fluorescence.

17 e of their specific binding to cellulose and intrinsic fluorescence.

18 es being detected that cannot be resolved by intrinsic fluorescence.

19 nctional mutants was also investigated using intrinsic fluorescence.

20 ent with the observed spectral properties of intrinsic fluorescence.

21 distinctive circular dichroism features, and intrinsic fluorescence.

22 ld be easily detected in gels based on their intrinsic fluorescence.

23 by large changes in Stokes radius (11 A) and intrinsic fluorescence (2-fold).

24 y and cross-linking activity, but had little intrinsic fluorescence (370/440 nm).

25 po-forms of CP by far-UV circular dichroism, intrinsic fluorescence, 8-anilinonaphthalene-1-sulfonic

26 R163K exhibited higher intrinsic fluorescence, a more negative molar ellipticit

27 Measurement of changes in intrinsic fluorescence allowed determination of a K(d) f

28 es also have high sensitivity, even for weak intrinsic fluorescence, allowing for the label-free imag

29 this report, we show that extraction of the intrinsic fluorescence allows us: (a) to determine the f

30 Such studies can be complicated by the intrinsic fluorescence and absorbance properties of CheA

31 esponsible for the majority of the protein's intrinsic fluorescence and all of the quenching that acc

32 ange characterized by a 2-fold change in the intrinsic fluorescence and an 11 A change in the Stokes

33 indicated by the substantial enhancement in intrinsic fluorescence and blue shift of fluorescent max

34 Temperature effects on intrinsic fluorescence and catalytic activity of hTS and

35 the stability of the R subunit was probed by intrinsic fluorescence and circular dichroism (CD) in th

36 major globular protein in kidney beans using intrinsic fluorescence and circular dichroism (CD).

37 ing and end-point structures as indicated by intrinsic fluorescence and circular dichroism, respectiv

38 Using measurements of protein intrinsic fluorescence and circular dichroism, we demons

39 mately 43 degrees C, monitored by changes in intrinsic fluorescence and circular dichroism.

40 The intrinsic fluorescence and difference absorption studies

41 Interestingly, spectral probes, such as intrinsic fluorescence and far-UV CD spectroscopy did no

42 oligomerization as determined by changes in intrinsic fluorescence and fluorescence resonance energy

43 nformational changes evidenced by changes in intrinsic fluorescence and in CD spectra and changes in

44 Rapid decreases in protein intrinsic fluorescence and increases in TNP-ATP fluoresc

45 Intrinsic fluorescence and iodide quenching in the prese

46 hanges to be probed by monitoring changes in intrinsic fluorescence and loss of binding of a low-mole

47 studied by fluorescence quenching of protein intrinsic fluorescence and nephelometry.

48 ctured, having a similar circular dichroism, intrinsic fluorescence and NMR spectrum as the protein d

49 ce anisotropy of rhodamine-labeled profilin, intrinsic fluorescence and nucleotide exchange, give the

50 matic residues (near-UV circular dichroism), intrinsic fluorescence and peptide binding activity are

51 The potential of intrinsic fluorescence and principal component analysis

52 mer formation, as demonstrated by changes in intrinsic fluorescence and quantitative native gel elect

53 t-index lens assembly for two-photon excited intrinsic fluorescence and second-harmonic generation im

54 vivo mouse tissue through the collection of intrinsic fluorescence and second-harmonic signal withou

55 Importantly, intrinsic fluorescence and sedimentation velocity analys

56 actin (W79) was generated that showed small intrinsic fluorescence and should be useful for studies

57 d by relative changes in iodide quenching of intrinsic fluorescence, and A-loop conformation was rank

58 Circular dichroism, intrinsic fluorescence, and differential scanning calori

59 nitoring changes in its absorption spectrum, intrinsic fluorescence, and fluorescence of the Nile red

60 were observed by way of circular dichroism, intrinsic fluorescence, and Forster resonance energy tra

61 infrared, differential scanning calorimetry, intrinsic fluorescence, and hydrogen-deuterium exchange

62 ion, we investigated plastic hydrophobicity, intrinsic fluorescence, and oligo attachment efficiency.

63 electrophoresis, nuclear magnetic resonance, intrinsic fluorescence, and proteolysis assays of wild-t

64 ere monitored by the changes in the receptor intrinsic fluorescence, and the data are consistent with

65 Return of enzymatic activity, intrinsic fluorescence, and the S1 pocket appeared to oc

66 th a 25% maximal glucose-related decrease in intrinsic fluorescence, and the saturation point was inc

67 ongly blue-shifted emission spectrum, higher intrinsic fluorescence, and weaker energy transfer from

68 ion experiments measuring lambda(max) of the intrinsic fluorescence as a function of guanidine hydroc

69 An intrinsic fluorescence assay shows that TH-2/3 also bind

70 Size exclusion chromatography and a simple intrinsic fluorescence assay were used to determine that

71 Furthermore, intrinsic fluorescence assays indicated that these metal

72 heme for mutant HIV-1 RT heterodimers, using intrinsic fluorescence assays.

73 In this method we use intrinsic fluorescence biomarkers and the phasor approac

74 rescence anisotropy of dansyl-labeled GroES, intrinsic fluorescence, bis-ANS binding, sedimentation v

75 dysplastic and nondysplastic BE in terms of intrinsic fluorescence, bulk scattering properties, and

76 ing near-UV circular dichroism, quenching of intrinsic fluorescence by acrylamide and interactions wi

77 Quenching of the intrinsic fluorescence by DNA and external dynamic quenc

78 ite-specific, ATP-dependent quenching of DNR intrinsic fluorescence by spin labels.

79 further elucidate the molecular basis of the intrinsic fluorescence change (and the source of the slo

80 , nucleotide analogue trapping kinetics, and intrinsic fluorescence changes accompanying nucleotide b

81 In this paper, we demonstrate that intrinsic fluorescence changes can be used to monitor th

82 To examine the structural basis of the intrinsic fluorescence changes that occur during the MgA

83 GTP gamma S-stimulated intrinsic fluorescence changes were completely abolished

84 Substrate-dependent intrinsic fluorescence changes were monitored in transpo

85 (2+) to apoFosA alters the UV absorption and intrinsic fluorescence characteristics of the protein su

86 on of HK97 in vitro by monitoring changes in intrinsic fluorescence, circular dichroism (CD), and SAX

87 Using intrinsic fluorescence, circular dichroism, and size exc

88 mutants were generated to permit the use of intrinsic fluorescence, circular dichroism, and ultravio

89 g tissue using two- and three-photon-excited intrinsic fluorescence combined with second harmonic gen

90 Intrinsic fluorescence combined with UV resonance Raman

91 An unprecedented class of macrocycles with intrinsic fluorescence consisting of phenolic trimers an

92 the assembly dependence of the site-specific intrinsic fluorescence data suggests that the CHC is par

93 ording to hydrogen exchange and stopped-flow intrinsic fluorescence data, unfolding of mAAT appears t

94 The association of activity loss with an intrinsic fluorescence decrease and loss of the S1-pocke

95 Conversely, the intrinsic fluorescence decrease observed at higher LM pe

96 , induced a blue shift in the lambda(max) of intrinsic fluorescence derived from residues in the cent

97 The application of intrinsic fluorescence detection with excitation in the

98 gnals of aptamer binding to urea in terms of intrinsic fluorescence differences and color changes sim

99 Intrinsic fluorescence due to tryptophan of the mutant p

100 Circular dichroism (CD) and intrinsic fluorescence emission (IFE) spectra of active

101 s dependently on the ionic strength, and the intrinsic fluorescence emission maxima of its single try

102 The intrinsic fluorescence emission of each single-Trp mutan

103 Differences in intrinsic fluorescence emission spectra were observed du

104 The intrinsic fluorescence enhancement observed upon binding

105 n nucleotide binding, demonstrating that the intrinsic fluorescence enhancement of smooth muscle myos

106 he Ca(2+)-bound active state as indicated by intrinsic fluorescence experiments.

107 k, we applied thioflavin T binding, tyrosine intrinsic fluorescence, fluorescence anisotropy measurem

108 Intrinsic fluorescence, FRET, and Fourier transform infr

109 ng using fluorescently labeled protein A and intrinsic fluorescence from fluorescein.

110 We report the enhanced intrinsic fluorescence from several proteins in proximit

111 affinity and specificity) with those of GFP (intrinsic fluorescence, high stability, expression and s

112 reactivity concomitant with disappearance of intrinsic fluorescence (IC(50) approximately 20 microM).

113 ryptase were investigated by analysis of (i) intrinsic fluorescence, (ii) inhibitor binding, and (iii

114 idence reveals the presence of a new type of intrinsic fluorescence in biomolecules that exists even

115 Intrinsic fluorescence in proteins is dominated by the t

116 ntributor to nucleotide-dependent changes of intrinsic fluorescence in smooth muscle myosin.

117 beled actin fluorescence or by a decrease in intrinsic fluorescence in the absence of pyrene-labeled

118 With their intrinsic fluorescence in the NIR2 regime and lack of ph

119 Candidates for the observed quench of intrinsic fluorescence in W512 KO-MDE include W29 and W3

120 able free amino groups, decreased tryptophan intrinsic fluorescence, increased molecular mass and Ric

121 ndings are as follows: 1) differences in the intrinsic fluorescence indicate that the Trp microenviro

122 ifferent short-chain phospholipids on enzyme intrinsic fluorescence indicates that anionic phospholip

123 M on polyacrylamide gels, differences in the intrinsic fluorescence intensity and solvent accessibili

124 In addition, the intrinsic fluorescence intensity of C403S is enhanced 3-

125 esterol/POPC large unilamellar vesicles, the intrinsic fluorescence intensity of sPLA2 shows an alter

126 Initial monitoring of changes in the intrinsic fluorescence intensity of vaults showed a 60%

127 ibrium unfolding transition was monitored by intrinsic fluorescence intensity, fluorescence anisotrop

128 unfolding can be monitored directly, because intrinsic fluorescence is quenched by denaturation.

129 nts with Trp at positions 15, 27 and 61, the intrinsic fluorescence is significantly quenched in the

130 Heparin affinity chromatography and intrinsic fluorescence kinetic analyses demonstrated tha

131 We have developed a label-free intrinsic fluorescence lifetime assay that uniquely can

132 red-shift in emission and an increase in the intrinsic fluorescence lifetime for the emitting state i

133 Intrinsic fluorescence, limited proteolysis, and molecul

134 This is the first demonstration that intrinsic fluorescence may be used to monitor calcium bi

135 (P = 1.9 x 10(-9)), was associated with skin intrinsic fluorescence measured by SCOUT DS (excitation

136 cellar or micellar state was investigated by intrinsic fluorescence measurement and dynamic light sca

137 Intrinsic fluorescence measurements confirmed their high

138 Rather, confocal microscopy, SDS-PAGE, and intrinsic fluorescence measurements indicated tight bind

139 Intrinsic fluorescence measurements of binding by the pu

140 Intrinsic fluorescence measurements of tryptophan showed

141 Circular dichroism and intrinsic fluorescence measurements revealed significant

142 Intrinsic fluorescence measurements revealed that oligom

143 Far UV CD and intrinsic fluorescence measurements show that this compa

144 Intrinsic fluorescence measurements showed that PT-(1-46

145 Intrinsic fluorescence measurements suggest the dissocia

146 Intrinsic fluorescence measurements suggested that Zn(2+

147 Intrinsic fluorescence measurements were performed to me

148 ecific GHS-R1a conformations, as assessed by intrinsic fluorescence measurements.

149 The importance of proton transfer on the intrinsic fluorescence observed in protein fibrils is si

150 -, and 3-h cohorts, respectively, and a mean intrinsic fluorescence of 0.035 vs. 0.023 mm(-1) (P < 0.

151 ects of loop sequence and loop length on the intrinsic fluorescence of 13 DNA GQSs.

152 The intrinsic fluorescence of 3-OST-1 was increased in the p

153 est or, in selected instances, the change in intrinsic fluorescence of a DNA binding agent itself and

154 Finally, we demonstrate that the intrinsic fluorescence of a large range of physical subs

155 The intrinsic fluorescence of a single tryptophan (Trp-698)

156 In common fluorogenic probes, the intrinsic fluorescence of a small-molecule fluorophore i

157 Intrinsic fluorescence of a tryptophan at residue 698 (T

158 Here, we use intrinsic fluorescence of a tryptophan located at the di

159 sing two assays, including one that uses the intrinsic fluorescence of a tryptophan residue, we have

160 Using the intrinsic fluorescence of all-trans-retinyl esters, noni

161 The intrinsic fluorescence of alpha-tocopherol and its effec

162 The intrinsic fluorescence of alphaBF61N was marginally lowe

163 Using the intrinsic fluorescence of anthralin, rapid accumulation

164 rate that the increases and decreases in the intrinsic fluorescence of antithrombin during heparin bi

165 Intrinsic fluorescence of ApoL1 supported the presence o

166 rt development of an assay that exploits the intrinsic fluorescence of Arabidopsis thaliana PCO trypt

167 The intrinsic fluorescence of betaLG was quenched by vitamin

168 mplex with BSA occurred through quenching of intrinsic fluorescence of BSA.

169 er common peroxidases, (2) monitoring of the intrinsic fluorescence of dityrosine to determine optimu

170 y(G) stimulated proteolysis and quenched the intrinsic fluorescence of EF-3.

171 Furthermore, the intrinsic fluorescence of fluorobodies correlates with b

172 Glomerular imaging relied on intrinsic fluorescence of GFP or DsRed, or on whole-moun

173 The intrinsic fluorescence of GQSs may be useful for nucleic

174 The intrinsic fluorescence of hexokinase in solution (excita

175 Specifically, by analyzing intrinsic fluorescence of human epithelial tissue as it

176 ine sensing which is based on monitoring the intrinsic fluorescence of in situ synthesized polydopami

177 l as for the determination of Kd's using the intrinsic fluorescence of LT.

178 Using two-photon microscopy and the intrinsic fluorescence of N-retinylamides, we showed tha

179 states by fluorescence quenching analysis of intrinsic fluorescence of native tryptophan (Trp) residu

180 etry and environment of its chromophore, the intrinsic fluorescence of OCP reveals changes in its ter

181 by the ligand urate, which also quenches the intrinsic fluorescence of PecS, indicating a direct inte

182 Two-photon excitation is used to probe the intrinsic fluorescence of peptide fragments through "dee

183 orms a ground-state complex by quenching the intrinsic fluorescence of PPO.

184 eagents, both the enzymatic activity and the intrinsic fluorescence of PR-DeltaPol are linearly depen

185 We used changes in the intrinsic fluorescence of profilin, CD spectroscopy, and

186 The intrinsic fluorescence of smooth muscle myosin is sensit

187 L-1 was studied by monitoring changes in the intrinsic fluorescence of sP-selectin upon binding to sP

188 l biophysical analysis demonstrated that the intrinsic fluorescence of SP600125 changed markedly upon

189 The intrinsic fluorescence of Ssa1p's only tryptophan residu

190 altered nucleotide-dependent changes in the intrinsic fluorescence of Ssa1p.

191 By taking advantage of the intrinsic fluorescence of SWG, we followed its fate in c

192 ed video-rate imaging of tumors based on the intrinsic fluorescence of SWNTs in the second near-infra

193 sedimentation equilibrium and velocity, and intrinsic fluorescence of the 2 Trp residues.

194 Basically, the intrinsic fluorescence of the amino acid tryptophan is e

195 sion difference is due to differences in the intrinsic fluorescence of the bound fluorophores rather

196 The intrinsic fluorescence of the catalytic portion of the c

197 is was carried out to ascertain the relative intrinsic fluorescence of the enzyme intermediates.

198 The intrinsic fluorescence of the intermediate suggested tha

199 Upon binding of Ca(2+), the intrinsic fluorescence of the mutant decreases approxima

200 x formation is accompanied by an increase in intrinsic fluorescence of the primers, allowing simple a

201 This used the intrinsic fluorescence of the protein to monitor the che

202 milar changes in the vibrational spectra and intrinsic fluorescence of the protein, which indicates t

203 te and is accompanied by a blue shift in the intrinsic fluorescence of the protein.

204 The intrinsic fluorescence of the six tyrosines located with

205 By utilizing the intrinsic fluorescence of the tryptophan (Trp) and tyros

206 The intrinsic fluorescence of the tryptophans could be used

207 termined by titrations of the alterations in intrinsic fluorescence of the variant kringles with the

208 ound; and (c) Trp222 contributes most to the intrinsic fluorescence of the wt-RI-subunit, while Trp18

209 Mg2+ altered the tryptophan intrinsic fluorescence of this polypeptide whereas Ba2+,

210 e formation in this part of the protein; the intrinsic fluorescence of this tryptophan was found to d

211 The intrinsic fluorescence of Tp38 was essentially unaltered

212 Thus the intrinsic fluorescence of Trp-11 provides a useful probe

213 s the formation of Meta III and quenches the intrinsic fluorescence of Trp265.

214 However, changes in the intrinsic fluorescence of truncated and Trp16 mutants up

215 ding to Rh using UV-visible spectroscopy and intrinsic fluorescence of tryptophan (Trp) 265 after ill

216 n constant estimated from the changes in the intrinsic fluorescence of tubulin was found to be consis

217 Spectroscopy examining the intrinsic fluorescence of tyrosine and tryptophan residu

218 o residues of tryptophan, which dominate the intrinsic fluorescence of unlabeled CF1.

219 Whereas the intrinsic fluorescence of W36-MDE is only slightly sensi

220 In this study, we used intrinsic fluorescence of wild type Abeta-(1-40), fluore

221 ese tryptophans was found to account for the intrinsic fluorescence of wild-type actin, indicating th

222 lcium titrations monitored by changes in the intrinsic fluorescence of Y138 in site IV showed that th

223 s study, we investigated measurements of the intrinsic fluorescence of yeast hexokinase as an assay f

224 glutenins and gliadins was analyzed through intrinsic fluorescence parameters, phase diagram method,

225 In addition, the intrinsic fluorescence polarization increases from 0.327

226 ptophan mutation at position 39 to create an intrinsic fluorescence probe and allow additional charac

227 on dynamics, using 2-aminopurine (Ap) as the intrinsic fluorescence probe and with femtosecond resolu

228 ng, we developed the ds-NIF (nucleoside with intrinsic fluorescence)-probe methodology for detection

229 turbation assay in which a change in the MAb intrinsic fluorescence produced by ligand binding was us

230 The proteins' similar intrinsic fluorescence properties and corresponding quen

231 nvironment, we have studied the steady-state intrinsic fluorescence properties of the purine and pyri

232 predicted binding to complement C3d and with intrinsic fluorescence properties to enable detection.

233 nm, and after purification, the QDs exhibit intrinsic fluorescence quantum yield efficiencies of 15

234 Immunological and intrinsic fluorescence quenching assays and the molecula

235 Binding affinities were characterized by intrinsic fluorescence quenching assays, isothermal titr

236 of 0.24 microM for the Efb-C3d binding using intrinsic fluorescence quenching assays.

237 Isothermal calorimetry and intrinsic fluorescence quenching studies show that oxida

238 nderwent normal, two-stage calcium-dependent intrinsic fluorescence quenching, indicating that a fold

239 g SDS-polyacrylamide gel electrophoresis and intrinsic fluorescence quenching.

240 Intrinsic fluorescence, reflectance, and light-scatterin

241 tryptophans at various locations to serve as intrinsic fluorescence reporters, i.e. as topological pr

242 ructure determined by circular dichroism and intrinsic fluorescence respectively were significantly a

243 Whereas the collective, intrinsic fluorescence response of E. coli CPS is largel

244 in characterization with enzyme kinetics and intrinsic fluorescence revealed that the C29S variant an

245 Near UV CD and intrinsic fluorescence revealed that the tryptophan resi

246 Intrinsic fluorescence showed that wild-type rFV(a2)-C2

247 S1-S4 domain by solution NMR, far-UV CD, and intrinsic fluorescence shows that this domain undergoes

248 Previous work, in which the increase in the intrinsic fluorescence signal was used to monitor iron r

249 ignature that was readily separated from the intrinsic fluorescence signature of tumor cells.

250 Analysis of intrinsic fluorescence spectra demonstrates that uric ac

251 Intrinsic fluorescence spectra for both proteins were al

252 tion coefficient mapping has been applied to intrinsic fluorescence spectra of colonic tissue for the

253 Then, we extract the intrinsic fluorescence spectra of sites from 35 patients

254 e emission peak, and causes quenching of the intrinsic fluorescence spectra of the proteins.

255 Intrinsic fluorescence spectra suggest that the associat

256 cularly, phospholipid/saposin C complexes by intrinsic fluorescence spectral shifts, fluorescence que

257 upon calcium binding, which was confirmed by intrinsic fluorescence spectroscopy and nondenaturing ge

258 Steady-state intrinsic fluorescence spectroscopy demonstrated that ph

259 Intrinsic fluorescence spectroscopy of purified proteins

260 ed from isothermal titration calorimetry and intrinsic fluorescence spectroscopy suggests that YKL-39

261 ptical cancer detection system that combines intrinsic fluorescence spectroscopy, diffuse reflectance

262 The highly sensitive method of intrinsic fluorescence spectroscopy, predicated on the m

263 was monitored by both circular dichroism and intrinsic fluorescence spectroscopy.

264 veloped for the on-chip recording of protein intrinsic fluorescence spectrum originating from aromati

265 Analysis of the intrinsic fluorescence, Stokes radius, thermal stability

266 Intrinsic fluorescence studies of the two mutants reveal

267 Intrinsic fluorescence studies were conducted to determi

268 Kinetic analysis, intrinsic fluorescence studies, and induced fit docking

269 d properties such as near- and far-UV CD and intrinsic fluorescence studies.

270 -UV circular dichroism spectrum and a higher intrinsic fluorescence than the heterodimer, demonstrati

271 We have characterized the changes in intrinsic fluorescence that the insulin receptor undergo

272 by monitoring the quenching of the protein's intrinsic fluorescence; the tartronate concentration dep

273 ith pyrene-labeled yeast actin, but not with intrinsic fluorescence, there is an overshoot in the flu

274 With the aid of two PNA analogues having intrinsic fluorescence, thiazole orange (TO)-PNA and [bi

275 al results confirmed that RES quenched ARA's intrinsic fluorescence through static quenching, indicat

276 An intrinsic fluorescence titration of various alpha-LA for

277 properties of rNK1/K1 were investigated via intrinsic fluorescence titration with kringle-specific o

278 spectroscopy, sedimentation equilibrium, and intrinsic fluorescence to monitor the reversible conform

279 Using a combination of intrinsic fluorescence to report ATP-induced rearrangeme

280 sines to the C-terminal domain of TBP allows intrinsic fluorescence to separately report on the struc

281 rmeability, direct membrane association, and intrinsic fluorescence to study the activities of purifi

282 We have used intrinsic fluorescence to test the hypothesis that phosp

283 domain of p53 (p53C) using light scattering, intrinsic fluorescence, transmission electron microscopy

284 n HCT116 cells was measured using the drug's intrinsic fluorescence under conditions that alter pH(i)

285 al pH of 5.6, measurement of the increase in intrinsic fluorescence upon iron release from the recomb

286 Changes in intrinsic fluorescence upon nucleotide and inositide bin

287 phans except W512 (W512 KO-MDE) decreases in intrinsic fluorescence upon nucleotide binding, demonstr

288 and 700 MPa, where a significant decrease in intrinsic fluorescence was also observed.

289 ells for intracellular injection using their intrinsic fluorescence was developed.

290 e treatment whereas a noticeable decrease in intrinsic fluorescence was evidenced up to 600 MPa and t

291 A complex, time-dependent change in enzyme intrinsic fluorescence was observed upon exposure to glu

292 targeting and design of assembly inhibitors, intrinsic fluorescence was used to probe assembly-depend

293 g of wild-type RIalpha (wt-RI), monitored by intrinsic fluorescence, was described previously.

294 tains no tryptophan residues and exhibits no intrinsic fluorescence, was harvested from CHO cells cot

295 Activity and intrinsic fluorescence were used to measure the stabiliz

296 t protein structural changes, as measured by intrinsic fluorescence, were much slower (t1/2 = 16 min)

297 uced dispersion analysis (FIDA), and in-line intrinsic fluorescence which enables rapid and detailed

298 nding; (b) a temperature-induced increase in intrinsic fluorescence, which reflects formation of a tr

299 , we found that all nine compounds decreased intrinsic fluorescence without affecting the overall UV-

300 yll a molecules, which confer on them a high intrinsic fluorescence yield.