ライフサイエンスコーパス: invertase

1 on (40-50%) in release of a secreted enzyme (invertase).

2 ters and a putative beta-fructofuranosidase (invertase).

3 aride must first be hydrolysed by the enzyme invertase.

4 and the high mannose-containing glycoprotein invertase.

5 unoisolated clathrin-coated vesicles contain invertase.

6 r the endodermis and phloem for soluble acid invertase.

7 fect on the retained activity of immobilized invertase.

8 Pex15p was fused to the N-terminus of mature invertase.

9 da repressor protein to the secreted protein invertase.

10 lation of SbVIN1, a gene encoding a vacuolar invertase.

11 a suitable support for the immobilisation of invertase.

12 lix that controls these steps by the Hin DNA invertase.

13 nts are shuffled by Rci, a site-specific DNA invertase.

14 reakdown through sucrose synthase instead of invertase.

15 ished their studies of sucrose hydrolysis by invertase.

16 d prokaryotic serine site-specific resolvase-invertases.

17 terminal domains compared with the resolvase/invertases.

18 rved for the differentially sugar-responsive invertases.

19 s encoding a SWEET transporter and cell wall invertases.

20 o each other and the FimE and HbiF bacterial invertases.

21 nthetic genes may have evolved from vacuolar invertases.

22 Upon rapid induction of yeast cytosolic invertase, a marked phenotype appears in developing leav

23 Antioxidant and invertase activities presented some desirable values.

24 nthase activity and reduced Suc synthase and invertase activities, leading to increased Suc contents.

25 Despite a considerable rise in invertase activity (200 units) during ripening of Shahan

26 ted well with an asymmetric increase in acid invertase activity across the pulvinus.

27 MdesGH32 has strong inulinase and invertase activity aiding in the breakdown of the plant

28 Both invertase activity and (1)(3)C import were greater in sh

29 In the transformed plants, invertase activity and a 70-kD cross-reacting protein we

30 (RS), high non-reducing sugars content, low invertase activity and high sucrose synthase (SuSy) acti

31 loem girdling on (1)(3)C and (1)(5)N import, invertase activity and polyphenol accumulation in juveni

32 This corresponded to an increase in invertase activity and the accumulation of phenolic comp

33 enile tissues was positively correlated with invertase activity at the treatment site and enhanced by

34 Analysis of invertase activity in nontuberizing and tuberizing stolo

35 carrot lines revealed very low acid-soluble invertase activity in rs/rs roots whereas neutral invert

36 Invertase activity is reduced in vitro in the presence o

37 Invertase activity is thought to play a regulatory role

38 Variation in acid invertase activity reflects multiple evolutionary events

39 However, cell wall-bound invertase activity remained high in the apical 1 to 2 mm

40 orms a boundary, compartmentalizing apoplast invertase activity to allow different embryo and endospe

41 The biological fibrils with invertase activity turn into microstructured catalysts a

42 Changes in endosperm invertase activity were complex and quantitatively do no

43 ected in significant reductions in cell wall invertase activity.

44 ross-reacting polypeptide, but was devoid of invertase activity.

45 70% of the sugars consumed were released by invertase activity.

46 ing both Asp-239 and Trp-47 homologs, has no invertase activity.

47 t yeasts [having normal (Y1) or reduced (Y2) invertase activity] were used to leaven the breads enric

48 gard, the high content of RS was due to high invertases activity.

49 ucrose and starch hydrolysis (e.g. cell wall invertase, alpha-amylase, and starch phosphorylase) were

50 Invertase, an enzyme responsible for sucrose metabolism,

51 invertase) of the signal toward its analyte invertase and a negligible nonspecific interaction of th

52 The activities of invertase and cell wall-modifying enzymes, namely pectin

53 r carboxypeptidase Y (CPY), accumulates both invertase and CPY in dense vesicles.

54 ontent of DAGE and the enzymatic activity of invertase and diastase in honey.

55 Despite evidence that yeast invertase and dry heat degrade inulin, the extent to whi

56 identically to wild type and show delays of invertase and Gas1p ER-to-Golgi transport identical to t

57 e Y trafficking defect, but the secretion of invertase and gp400/hsp150 is not significantly affected

58 temperature; however, other proteins (i.e., invertase and HSP150) in these and other COPI mutants we

59 ulate an internal pool of fully glycosylated invertase and mature alpha-factor, while processing and

60 ycoside hygromycin B, and exhibit diminished invertase and Sim1 glycosylation.

61 elongation intermediates were released from invertase and similarly analyzed.

62 ructose, and glucose levels alongside stable invertase and sucrose phosphate synthase activities.

63 ions to get Tyr-Tyr covalent binding between invertase and the support were determined using a photoc

64 ptide followed by the coding region of yeast invertase and the transmembrane domain and cytoplasmic t

65 nd rse1-1 mutants accumulate the ER forms of invertase and the vacuolar protease CPY at restrictive t

66 the quantitative conversion of analytes into invertase and then glucose, which can be measured by an

67 ular C terminus can be processed to secreted invertase and this fraction is constrained to 2-3% by a

68 ncodes an integrase related to the resolvase/invertases and is evolutionarily and mechanistically dis

69 apid change in the regulation and balance of invertases and Suc synthases that could have an immediat

70 inding domain of the Hin family of bacterial invertases and to homeodomain proteins.

71 ersatile and include integrases, resolvases, invertases and transposases.

72 ndent fashion, the transcription of vacuolar invertase, and a wak2 mutant alters the normal pectin re

73 y owing to a jasmonic acid-induced cell wall invertase, and is limited by phloem sucrose availability

74 ferent sets of cargoes typified by Bgl2p and invertase are delivered to the plasma membrane for secre

75 MYB transcription factors and invertase are implicated in fruit color and sugar compos

76 The archetypal resolvase/invertases are highly regulated, only affect resolution

77 Invertases are regulated at the posttranslational level

78 biological role has been associated with the invertase as of yet.

79 stained by both sucrose synthase and neutral invertase, associated with minimal futile cycling.

80 i forms of both CPY and the secreted protein invertase at the nonpermissive temperature.

81 a is involved in degradation of the vacuolar invertase AtFruct4 in aging tissues.

82 The Hin DNA invertase becomes catalytically activated when assembled

83 invertase-repressor fusions, like wild-type invertase, behave as soluble proteins in the ER lumen.

84 inetics was based on the properties of yeast invertase (beta-fructo-furanosidase, EC 3.2.1.26), whose

85 Invertase (beta-fructosidase, EC 3.2.1.26) hydrolyzes su

86 A range of enzymes, namely beta-glucosidase, invertase, beta-galactosidase, and catalase, are encapsu

87 l studied serine recombinases, the resolvase/invertases, bring two recombination sites together in a

88 stingly, CPY-invertase hybrid proteins, like invertase but unlike CPY, escaped the sec21 ts mutant ER

89 group, are similar in size to the resolvase/invertases but have the DNA binding domain N-terminal to

90 ing an Och1p-invertase fusion do not secrete invertase, but those expressing an Och1p-Kex2p site-inve

91 e at about a 2-fold molar excess inactivated invertase by modifying both of the enzyme's cysteine res

92 Rapid repression of the Ivr1 and Ivr2 maize invertases by low oxygen was evident in root tips within

93 d with the production of a metabolic enzyme, invertase, by Saccharomyces cerevisiae, which catalyses

94 ity with allelic variants within an alkaline invertase candidate gene LpcAI.

95 on of DNA in the sample into glucose through invertase-catalyzed hydrolysis of sucrose.

96 rtons) in certain Type I R-M systems undergo invertase-catalyzed inversions.

97 It is proposed that an activated invertase causes the immediate loss of the plasmid in mo

98 ut activities of neutral invertase, vacuolar invertase, cell wall-bound invertase, fructose kinase, a

99 table aggregate and activities of urease and invertase compared to conventional nitrogen rate, which

100 , released from wheat fructan and sucrose by invertase, compared to maltose is, however, not document

101 is based on target-dependent binding of cDNA-invertase conjugate with the analyte DNA, thereby transf

102 d release of invertase from a functional-DNA-invertase conjugate.

103 In both assay methods, with invertase conjugates as the link, quantitative detection

104 These data suggest that soluble acid invertase controls sugar composition in tomato fruit and

105 The released invertase converts sucrose into glucose, which is detect

106 Cell wall invertases (CWINVs) catalyze the irreversible hydrolysis

107 Cell wall invertases (cwINVs), with a high affinity for the cell w

108 forming the resulting fusion library into an invertase-deficient yeast strain and plating the transfo

109 esults demonstrate that unique resolvase and invertase derivatives can be developed to site-specifica

110 ino acid residues that mediate resolvase and invertase DNA sequence specificity.

111 evealed the molecular basis of resolvase and invertase DNA sequence specificity.

112 low temperature, defects in glycosylation of invertase, dominant lethality, fluoride sensitivity, and

113 thase in the embryo was greater than that of invertase during development.

114 that ZM-INVINH1 interacts with an apoplastic invertase during early kernel development.

115 increased transcript abundance of a VACUOLAR INVERTASE during ripening was consistent with sugar stor

116 ic isotope fractionation associated with the invertase (EC 3.2.1.26) and glucose isomerase (EC 5.3.1.

117 A-3) was used for covalent immobilization of invertase (EC 3.2.1.26).

118 honey standards for moisture, free acidity, invertase, electrical conductivity, and HMF, as these pa

119 We show here that a cell-wall invertase encoded by the Incw1 gene is regulated at both

120 of phage integrases related to the resolvase/invertase enzymes.

121 Man10GlcNAc and Man11GlcNAc species from invertase expressed in Pichia pastoris showed three and

122 Finally, we note that glucose repression of invertase expression in wild-type cells produces a strat

123 An association of invertase expression with generative tissue, both in veg

124 ugh incomplete cell separation, 10 increased invertase expression, none imported sucrose, and 11 incr

125 ng sucrose before hydrolysis, and increasing invertase expression.

126 Invertase family members reside on segmental duplication

127 catalytic domain derived from the resolvase/invertase family of serine recombinases and a custom-des

128 Hyperactivated variants of the resolvase/invertase family of serine recombinases function without

129 catalytic domains derived from the resolvase/invertase family of serine recombinases fused to Cys2-Hi

130 Members of the resolvase/invertase family of site-specific recombinases require s

131 gulatory divergence is characteristic of the invertase family.

132 ily and the serine recombinases or resolvase/invertase family.

133 These DNA invertases flip promoter regions in their immediate down

134 od is based on the target-induced release of invertase from a functional-DNA-invertase conjugate.

135 When applied to a sample of invertase from Aspergillus nidulans, the method indicate

136 Piv, a site-specific invertase from Moraxella lacunata, exhibits amino acid h

137 ion and characterization of an intracellular invertase from Trichoderma virens (TvInv) important for

138 es and involves at least three conserved DNA invertases from two evolutionarily distinct families.

139 vertase, vacuolar invertase, cell wall-bound invertase, fructose kinase, and hexokinase were unaffect

140 Cells expressing an Och1p-invertase fusion do not secrete invertase, but those exp

141 The Och1p-Kex2p site-invertase fusion protein is cleaved with a half-time of

142 se, but those expressing an Och1p-Kex2p site-invertase fusion protein secrete high levels of invertas

143 ll surface protein can synthesize a secreted invertase fusion protein that can rescue the mutant, and

144 ansformed with the promoter of an apoplastic invertase gene (invGE) linked to a reporter gene also re

145 induce the expression of the potato vacuolar invertase gene (VInv) and cause reducing sugar accumulat

146 1, 2 and 3) showed that sequences for maize invertase gene and for events MON810 and TC1507 were eas

147 We show here that invertase gene expression and the invertase-sucrose (Suc

148 tional processing was observed during normal invertase gene expression in potato.

149 ic fruit expressing a constitutive antisense invertase gene had increased sucrose and decreased hexos

150 no effect on the expression of the cell wall invertase gene in fluorescent carrot cells containing ar

151 cDNA cloning vector which carries a modified invertase gene lacking its leader sequence is used in co

152 rate that silencing the potato vacuolar acid invertase gene VInv prevents reducing sugar accumulation

153 es was located adjacent to the 5' end of the invertase gene, inv.

154 ealed asymmetric induction of one maize acid invertase gene, Ivr2, consistent with transcriptional re

155 omyces cerevisiae deleted for its endogenous invertase gene.

156 m the phloem to the apoplast where cell wall invertases generate monosaccharides for uptake and utili

157 The organisation of two invertase genes (invGE and invGF) linked in direct tande

158 ed together with multiple copies of vacuolar invertase genes and a transposable element on two barley

159 We show that two invertase genes in potato, like most other plant inverta

160 rtase genes in potato, like most other plant invertase genes, include a very short second exon of 9 b

161 In potato invertase genes, the constitutively included, 9-nucleoti

162 ng deletion polymorphisms in multiple linked invertase genes.

163 hich differs from previously described plant invertase genes; while intron locations are conserved be

164 hyperactivated catalytic domain from the DNA invertase Gin and an optimized TALE architecture.

165 mming of the DNA sequence specificity of the invertase Gin from bacteriophage Mu and Tn3 resolvase fr

166 ed activated mutant (M114V) of the G-segment invertase (Gin) in which one dimer half is rotated by 26

167 zyme sequential reaction was performed using invertase, GOX, and HRP.

168 lta gal83Delta strain is unable to derepress invertase, grows poorly on alternative carbon sources an

169 ctions (dehydrogenase > cellulase > urease > invertase > nirS) and increases the potential nitrificat

170 This yeast invertase had plant-specific complex glycans, indicating

171 Using the protein invertase, Hardklor identifies 18O-labeled peptide isoto

172 genic plants with altered extracellular acid invertase have highly disturbed growth habits.

173 jor clades of the tyrosine and serine family invertases homologs from 16 bacterial phyla, which poten

174 Interestingly, CPY-invertase hybrid proteins, like invertase but unlike CPY

175 ys showing the highest enzyme activity after invertase immobilisation.

176 ertase fusion protein secrete high levels of invertase in a Kex2p-dependent manner.

177 gated the role of intracellular soluble acid invertase in plant and fruit development.

178 ions also had greatly reduced levels of acid invertase in ripe fruit.

179 Transgenic plants were generated with yeast invertase in the cell walls to prevent Suc loading by th

180 Inactivation of the invertase in the donor strain resulted in a 1,000-fold i

181 stolons revealed a marked decline in soluble invertase in the subapical region of swelling stolons, c

182 the expression of a membrane-anchored yeast invertase in transgenic plants.

183 itation led to a down-regulation of vacuolar invertases in all plants, which resulted in an augmentat

184 disruption of sucrose cleavage by cell wall invertases in developing ovaries.

185 indicate that epigenetic switches driven by invertases in the epigenetic invertons broadly operate i

186 ic analysis of the fructosyltransferases and invertases in the Poaceae showed that the fructan biosyn

187 belongs to a small gene family of apoplastic invertases in tomato (Lycopersicon esculentum), is a qua

188 For grape invertase, in parallel with deglycosylated peptides anal

189 which includes transposon resolvases and DNA invertases, in that they utilize two simple but differen

190 Miniature1 (Mn1) locus encodes the cell wall invertase INCW2, which is localized predominantly in the

191 on at the Mn1 locus that encodes a cell wall invertase (INCW2) that localizes exclusively to the basa

192 ed in a 3, 000-fold reduction in the kcat of invertase indicating that Glu-204 plays a major role in

193 nt and identify the polymorphism in vacuolar invertase inhibitor (INH2) gene from Indian non-processi

194 We found that in maize (Zea mays), an invertase inhibitor homolog (ZM-INVINH1) is expressed ea

195 nsported by dense exocytic vesicles, such as invertase, into light exocytic vesicles, whereas transpo

196 sucrose in cells in which the only source of invertase is a C-terminal fusion to a transmembrane prot

197 Vacuolar invertase is a key enzyme of sugar metabolism in grape b

198 mutant cheater strain that does not produce invertase is able to take advantage of and invade a popu

199 Invertase is an enzyme that is widely distributed among

200 ged fusion proteins was constructed in which invertase is appended to the Golgi-luminal carboxy termi

201 roteomic approach demonstrates that vacuolar invertase is glycosylated at all twelve potential N-glyc

202 This DNA invertase is necessary for the inversion of at least 13

203 f RAG1 with sequence similarity to bacterial invertases is essential for DNA binding.

204 Piv, a unique prokaryotic site-specific DNA invertase, is related to transposases of the insertion e

205 e in Saccharomyces cerevisiae, which encodes invertase, is repressed about 200-fold by high levels of

206 The external invertase isoform 1 (EINV1) was immobilised on eight dif

207 s not occur in the Rs wild-type acid-soluble invertase isozyme II allele, it does occur elsewhere in

208 t intron near the 5' end of the acid soluble invertase isozyme II gene of rs/rs carrots.

209 While the wild-type acid-soluble invertase isozyme II transcript (ca. 2 kb) was detected

210 idate locus for carrot vacuolar acid-soluble invertase isozyme II.

211 Increased invertase levels in the stressed leaf meristem, on the o

212 way: A trichome-expressed, neofunctionalized invertase-like enzyme, SnASFF1, converts BAHD-produced a

213 44% of the sugars consumed were generated by invertase-mediated degradation of fructan, raffinose and

214 sess glucose flux differences between cells, invertase-mediated sucrose hydrolysis in vivo, delivery

215 Invertase-mediated sugar release seems to be crucial dur

216 TB2 reduced inclusion/splicing of the potato invertase mini-exon splicing reporter, indicating that t

217 This occurs in the potato invertase mini-exon via the polypyrimidine tract and ass

218 a Kex2p cleavage site between the Och1p and invertase moieties to monitor transit to the Kex2p-conta

219 to distal compartments is not induced by the invertase moiety, since noninvertase fusion constructs e

220 in G (IgG) antibody genetically fused to two invertase molecules.

221 In addition to expected genes such as invertases, natural variation was identified in key C4 m

222 ose synthase of soybean and a cell wall acid invertase of carrot.

223 e amino acids of a motif highly conserved in invertases of diverse origin.

224 binase, termed Inv, was highly homologous to invertases of the Din family.

225 Bacterial DNA invertases of the serine site-specific recombinase famil

226 ensor was only 6.1% (probed with an oxidized invertase) of the signal toward its analyte invertase an

227 uces the expression and activity of vacuolar invertase, often a key factor in turgor and expansion.

228 do not complement for Moraxella lacunata Piv invertase or IS492 MooV transposase activities.

229 se entering the fruit can be accomplished by invertase or sucrose synthase.

230 Invertase overproduction increases extracellular hexose

231 he major pilin subunits (tfpQ/I) and the DNA invertase (piv), which determines pilin type expression.

232 designed and highly convergent resolvase and invertase populations in the context of engineered zinc-

233 ne fetuin, bovine thyroglobulin, and several invertase preparations from wild-type and mutant yeast s

234 n be imported into the cell, serving to make invertase production and secretion a cooperative behavio

235 The Hin DNA invertase promotes a site-specific DNA recombination rea

236 sed appropriately upstream of this defective invertase provide the necessary signals to restore secre

237 In contrast to the resolvase/invertase recombination systems--where there are strict

238 stream partially prevented the abortion, but invertase regulated the synthesis of ovary starch and pa

239 is adjacent to mpi, encoding the global DNA invertase regulating capsular polysaccharide biosynthesi

240 We have named these genes irg for invertase-related gene family.

241 terest to a yeast (Saccharomyces cerevisiae) invertase reporter gene, transforming the resulting fusi

242 (a) The invertase-repressor fusions, like wild-type invertase, b

243 amily of site-specific recombinases--the DNA invertase/resolvase family--that catalyze inversion or d

244 cysteines with alanines revealed that C108A invertase retained full activity whereas C205A was reduc

245 ) released with peptide-N-glycosidase F from invertase secreted by Deltaalg9 yeast showed its structu

246 These cells are defective in invertase secretion and accumulate vesicles similar to t

247 effects of ste24- and spf1-null mutations on invertase secretion are additive, cell generation time i

248 rictive temperature and exhibit a pattern of invertase secretion comparable with sec14(ts) strains.

249 The kinetics of invertase secretion or transport of alkaline phosphatase

250 was found wild type in this mutant, although invertase secretion was impaired.

251 defects in chitin and cell wall deposition, invertase secretion, and fluid phase endocytosis.

252 on of transport vesicles and the decrease in invertase secretion.

253 xtensively glycosylated, indicating that the invertase segment inserted at these Akr1p sites is lumin

254 ity columns, i.e. asialofetuin-Sepharose and invertase-Sepharose.

255 rols, and high-mannose structures from yeast invertase served as negative controls.

256 mportant physiological role of Saccharomyces invertase (SInv) and the historical relevance of this en

257 Hin is a member of the DNA invertase subclass of serine recombinases that are regul

258 Theoretically advantageous reductions in the invertase/Suc synthase balance thus resulted.

259 ere motivated by the potential for a reduced invertase/Suc synthase balance to alter the impact of re

260 Portions of the yeast protein invertase (Suc2p) were inserted in-frame at 10 different

261 tase activity in rs/rs roots whereas neutral invertase, sucrose synthase and sucrose phosphate syntha

262 here that invertase gene expression and the invertase-sucrose (Suc) synthase ratio decrease abruptly

263 we report that FimX, an ExPEC-associated DNA invertase that regulates the major virulence factor type

264 Therefore, in addition to invertase, the growing embryo itself has a potential to

265 ructural groups represented by the resolvase/invertases, the large serine recombinases and relatives

266 Because yeast use invertase to hydrolyze sucrose extracellularly and impor

267 where sucrose is hydrolysed by an apoplasmic invertase to produce a mixture of sucrose, glucose and f

268 own to rapidly encounter the TGN glycosylate invertase to the same extent as wild-type cells, indicat

269 o mechanisms for suppressing the benefits of invertase to those who exploit it.

270 ) plants expressing a constitutive antisense invertase transgene grew identically to wild-type plants

271 rogenase) and sugars (sucrose synthase, acid invertase, trehalose-6-phosphate synthase, trehalose-6-p

272 sis of a deletion mutant of one of these DNA invertases, tsr15 (aapI), which resulted in the promoter

273 ression is regulated at one level by the DNA invertase Tsr19, which is encoded by a gene immediately

274 By means of sucrose and invertase uptake experiments, we have also shown that ac

275 t a novel strategy for the immobilization of invertase using amyloid-like fibrils as a support.

276 in silenced fruit, but activities of neutral invertase, vacuolar invertase, cell wall-bound invertase

277 Furthermore, a defect in invertase vesicle trafficking caused by vps1Delta or pep

278 mal amplification method with a thermostable invertase, we can directly transduce Middle-East respira

279 In a previous study on yeast invertase, we identified Asp-23 through the procedures o

280 gamma delta resolvases and the phage Mu Gin invertase, we used substrates that provided some but not

281 ranscripts for sucrose synthase and vacuolar invertase were both observed in the same cortical cells

282 The Km and Vmax values of immobilized invertase were found to be 39.4mmol/L and 349.5mmol/L mi

283 he structure and the catalytic properties of invertase were preserved, while Km values were slightly

284 dified secretory pathway cargos, Hsp150p and invertase, whereas stt4(ts) cells exhibit no detectable

285 ypeptidase Y, as well as in the secretion of invertase, which accumulates as a core-glycosylated form

286 This step was mediated by invertase, which had low activity.

287 the association of transcripts for vacuolar invertase with polyribosomes did not change over this pe

288 orresponded to genes encoding a soluble acid invertase with potential vacuolar targeting, which we ge