コーパス検索結果 (１語後でソート)
通し番号をクリックするとPubMedの該当ページを表示します
1 ts: MTSET+, MTSES-) and an alkylating agent (iodoacetamide).
2 d increased resistance against alkylation by iodoacetamide.
3 ree thiol molecules have been alkylated with iodoacetamide.
4 lenesulfonic acid and tetramethylrhodamine-5-iodoacetamide.
5 the photocross-linking reagent, benzophenone-iodoacetamide.
6 oxylic acid, N-ethylmaleimide, spermine, and iodoacetamide.
7 modification of cysteine residues with [14C]iodoacetamide.
8 Cys-961, Cys-995) were readily accessible to iodoacetamide.
9 in sequencing after modification with [1-14C]iodoacetamide.
10 ha-p-tosyl-L-lysine chloromethyl ketone) and iodoacetamide.
11 detected after treatment of the enzyme with iodoacetamide.
12 O(-), nitrated complex II was alkylated with iodoacetamide.
13 ble and were eliminated by pretreatment with iodoacetamide.
14 of actin labeled at Cys374 with erythrosine iodoacetamide.
15 s more reactive toward free cysteine than is iodoacetamide.
16 ing ERK2 proteins subjected to alkylation by iodoacetamide.
17 rential alkylation with N-ethylmaleimide and iodoacetamide.
18 e, 1-Cl-2,4-dinitrobenzene, and biotinylated iodoacetamide.
19 yme when thiolated by GSSG or alkylated with iodoacetamide.
20 tol followed by alkylation with radiolabeled iodoacetamide.
21 nd alkylated the free sulfhydryl groups with iodoacetamide.
22 eta-UP was very sensitive to inactivation by iodoacetamide.
23 cleophosmin was inhibited in the presence of iodoacetamide (10 mM), free (+)-avrainvillamide (100 mic
26 We labeled alphaA-crystallin with stilbene iodoacetamide (4-acetamido-4'-((iodoacetyl)amino)stilben
27 with different cysteine alkylating reagents: iodoacetamide, 4-vinylpyridine, and (3-acrylamidopropyl)
28 ly alkylated with acrylamide (+71 Da) versus iodoacetamide (+57 Da) have substantial chromatographic
29 wo heterobifunctional photoaffinity reagents iodoacetamide 9 and dansyl derivative 10 were prepared.
30 in the apoenzyme and that the binding of the iodoacetamide (a truncated analogue of the citrulline pr
33 ilar results were obtained on the photolyzed iodoacetamide-alkylated yeast GR, which was evaluated by
34 of the catalytic cysteine (SufS(C361A)) and iodoacetamide alkylation of SufU support the occurrence
35 ver, the measurement of the pH dependence of iodoacetamide alkylation revealed a pK value of 6.6 for
37 use of pan cysteine-reactive probes such as iodoacetamide alkyne combined with biotinylation via cop
38 cysteines more effectively than conventional iodoacetamide-alkyne, and to image oxidized thiols by co
39 nzyme of a thiol-reactive FRET probe, pyrene iodoacetamide, allowed us to monitor the binding by FRET
41 emically modified Cys and His residues using iodoacetamide and diethyl pyrocarbonate, respectively.
42 and Ba(OH)(2), blocking cysteine residues by iodoacetamide and enzymatic O-deglycosylation prior to D
43 ively, were each labeled with benzophenone 4-iodoacetamide and exchanged into myosin in their phospho
44 1 and Grx2 were sensitive to inactivation by iodoacetamide and H(2)O(2) and exhibited similar pH depe
45 n are not accessible to all electrophiles as iodoacetamide and internally applied 2-(trimethylammoniu
46 d identified 120 and 37 unique peptides with iodoacetamide and iodoacetic acid adducts, respectively.
49 he implementation of an alkylating step with iodoacetamide and optimization of the incubation tempera
51 ysteine-34 of HSA was permanently blocked by iodoacetamide and the primary amines were temporarily ma
53 The cleavage of DSEB/RF-C140 is prevented by iodoacetamide and the specific caspase-3 inhibitor, tetr
55 sfer was inhibited by treatment of IscU with iodoacetamide, and (35)S was released by reducing reagen
56 induced modification of fibrillarin to 2-ME, iodoacetamide, and hydrogen peroxide suggested interacti
57 nzyme was inactivated when preincubated with iodoacetamide, and protected from inactivation when preQ
58 leimide but not to the thioreactive compound iodoacetamide; and importantly, low concentrations of Tr
59 copy of TnC((E59D,D75Y)) labeled with 2-[(4'-iodoacetamide)-aniline]naphthalene-6-sulfonic acid showe
60 Measurement of the enzymatic inhibition by iodoacetamide as a function of pH showed maximum inhibit
62 ysteine probes from chemical modification by iodoacetamide as a means to obtain a surface map of TIP4
63 e compounds, p-chloromercuribenzoic acid and iodoacetamide, as well as the structure of a cysteine to
65 d FRET in a series of mutants bearing pyrene iodoacetamide at different positions, and we measured th
67 rescence from the triplet probe erythrosin-5-iodoacetamide attached to sulfhydryls in rabbit skeletal
69 etry following chemical modification with an iodoacetamide-based reagent over a range of pH buffers.
70 he facts that H(2)O(2) and biotin-conjugated iodoacetamide (BIAM) selectively and competitively react
73 aglandin J(2), 2,4-dinitrochlorobenzene, and iodoacetamide] correlates with the selective modificatio
74 anisotropy of the triplet probe erythrosin-5-iodoacetamide covalently attached to cysteine 374 of act
75 ropy (rFA) of the triplet probe erythrosin-5-iodoacetamide covalently bound to Cys-374 of skeletal mu
78 mutants are hexa-alkylated upon addition of iodoacetamide, demonstrating accessibility and full reac
79 Lysozyme (T4L) doubly spin-labeled with the iodoacetamide derivative of DZD, we observe the increase
82 annotated cysteines were identified by both iodoacetamide-desthiobiotin and methylsulfonylbenzothiaz
83 abeled at Cys-84 with tetramethylrhodamine-5-iodoacetamide dihydroiodide was passively exchanged for
84 30 minutes, the inhibition of CK activity by iodoacetamide does not limit left ventricular chamber co
85 ersible, time-dependent decrease in stilbene iodoacetamide emission intensity and a concomitant incre
90 8C, and S59C) were prepared, and fluorescein iodoacetamide (FI) was selectively conjugated to the sid
92 photoactivatable cross-linker benzophenone-4-iodoacetamide, followed by reconstitution and UV irradia
94 ure or metabolic inhibitors, sodium azide or iodoacetamide, have little influence on the translocatio
95 teine moiety is blocked by pretreatment with iodoacetamide, hemin binding is not affected, whereas dr
96 unrelated cysteine-modifying agents such as iodoacetamide (IA) and (2-aminoethyl)methanethiosulphona
97 Ten-day-old male rats received 0.2 mL 0.1% iodoacetamide (IA) in 2% sucrose daily by oral gavages f
98 ations by mPEG(5000)-maleimide (PEG-mal) and iodoacetamide (IA) under denatured condition, using extr
100 eta93Cys is modified by sulfhydryl reagents, iodoacetamide (IAA) and N-ethylmaleimide (NEM), respecti
101 ethyl]1-sulfo-5-naphthylamine (IAEDANS), and iodoacetamide (IAA) were used to selectively modify the
103 insensitive to iodoacetic acid (IAAcid) and iodoacetamide (IAAmide), though IAAmide-treated receptor
107 the effectiveness of N-ethylmaleimide (NEM), iodoacetamide (IAM), and iodoacetic acid (IAA) in alkyla
108 first block reduced thiols in extracts with iodoacetamide (IAM), and then we sequentially reduce and
109 on, the sulfhydryls are usually reacted with iodoacetamide (IAM), iodoacetic acid (IAA), or another e
110 comparison of three derivatization reagents: iodoacetamide (IAM), N,N-dimethyl-2-chloro-ethylamine (D
113 reagents such as N-ethylmaleimide (NEM) and iodoacetamide in a dose-dependent and time-dependent man
115 ammonium acetate (AmAc) in rat chow or 0.1% iodoacetamide in drinking water for 2-3 weeks underwent
116 and E2 cysteinyl residues were labeled with iodoacetamide in the native virus particle and analyzed
117 mutant enzymes, as shown by pH profiles for iodoacetamide inactivation of the respective enzymes, cl
119 Sp1 can be inhibited by N-ethylmaleimide and iodoacetamide, indicating that a cysteine protease media
120 ve, the pH-dependent incorporation of [(14)C]iodoacetamide into this mutant exhibits a pK(a) = 7.62.
122 cted agents, N-chlorosuccinimide, elastinal, iodoacetamide, iodoacetic acid, and phenylglyoxal gave s
123 , the pH-inactivation profile of 3CLpro with iodoacetamide is indicative of an ion-pair mechanism.
125 e monoethylene ketal in five simple steps to iodoacetamide ketone 10, which was cyclized in good yiel
126 icrosecond rotational dynamics of erythrosin-iodoacetamide-labeled actin strongly bound to single-hea
127 icrosecond rotational dynamics of erythrosin-iodoacetamide-labeled actin with strongly bound myosin V
129 lation caused a 48% decrease in biotinylated iodoacetamide-labeled Ras that was reversed by dithiothr
130 ications reflected by decreased biotinylated iodoacetamide labeling and evidence of specific irrevers
132 itrobenzoic acid) (Nbs2), and lucifer yellow iodoacetamide (LYI), suggests that sulfhydryl groups mig
133 on ionization time-of-flight spectroscopy of iodoacetamide-modified DmGCLM as well as examination of
135 plete mapping of total tryptic digest of the iodoacetamide-modified oat phytochrome A (phyA), the mol
136 cysteines cannot be distinguished with [14C]iodoacetamide, N-[(14)C]ethylmaleimide, or IAEDANS ([5-(
137 ity of the active-site Cys161 residue toward iodoacetamide nor the rate of unidirectional transfer of
138 D1s with TCEP increased the accessibility to iodoacetamide of cysteine residues that normally partici
139 in V-117 mutant was labeled with fluorescein iodoacetamide on its single N-terminal cysteine residue;
140 arable to or higher than the most often used iodoacetamide on peptides or maleimide on the antibody t
141 er induction of mild diffuse gastritis using iodoacetamide or creating gastric ulcers by injecting 60
142 al buffers, sulfinic acids do not react with iodoacetamide or disulfides, enabling selective alkylati
144 The demonstration that either treatment with iodoacetamide or replacement of the motif cysteine with
145 ompeting with cysteine-reactive maleimide or iodoacetamide reagents, the fast cellular uptake of VTT
147 on of hydroxylamine-treated ASGP-R with [14C]iodoacetamide resulted in the specific incorporation of
148 t, the pH dependence of SrtA inactivation by iodoacetamide revealed a single ionization for inactivat
149 ed proteins treated with N-ethylmaleimide or iodoacetamide revealed that all cysteines form covalent
150 Derivatization of gammaS-crystallin with iodoacetamide showed reaction at only six of the seven c
151 luoroalkyl tag, N-[(3-perfluorooctyl)propyl] iodoacetamide, significantly increased their hydrophobic
154 group-specific protease inhibitors, such as iodoacetamide, suggest an active-site cysteine or histid
155 ed and solubilized in the presence of 100 mM iodoacetamide, suggesting that it is not unique to rat l
156 fied by the application of cysteine-reactive iodoacetamide, suggesting that LSL may be deployed to sc
157 presence of the thiolate scavenging reagent, iodoacetamide, suggesting that the role of O2 is in oxid
159 of apoptosis, is blocked by the addition of iodoacetamide to cells prior to the onset of apoptosis,
160 67C was then treated with bromoethylamine or iodoacetamide to insert aminoethylcysteine (AEC) or carb
161 eta tubulin were alkylated with an excess of iodoacetamide to prevent artifactual generation of disul
162 This was achieved by applying a low dose of iodoacetamide to selectively inhibit the creatine kinase
164 be readily alkylated in the native SPL by an iodoacetamide treatment, suggesting that it is accessibl
165 d by reduction with NaBH(4), alkylation with iodoacetamide, trypsin digestion, and HPLC separation of
167 The fluorescent probe tetramethylrhodamine iodoacetamide was attached to cysteine residues substitu
168 Since the fluorescent reagent N-(1-pyrene)iodoacetamide was first used to label skeletal muscle ac
170 te synthetase (FPGS) that were reactive with iodoacetamide were located in peptides that were highly
171 enzyme, we labeled SERCA with erythrosin 5'-iodoacetamide, which binds to the phosphorylation domain
172 ications, arising from protein alkylation by iodoacetamide, which is commonly used in the standard pr
174 cence lifetime of the actin-bound erythrosin iodoacetamide: while muscle S1 increases the lifetime, s
175 t not the oxidized enzyme, is inactivated by iodoacetamide with alkylation of 2.7 cysteine residues/s
176 ne serum albumin (BSA) was modified by (14)C-iodoacetamide, with and without glutathione present, pro
177 ce all 22 half-cystines can be modified with iodoacetamide without reduction of the chromoprotein, it