ライフサイエンスコーパス: ion selectivity

1 rrowest part of the open pore) control metal ion selectivity.

2 ly controls gating, but also regulates Orai1 ion selectivity.

3 as the effect of chelate ring size on metal ion selectivity.

4 ion coordination site that is essential for ion selectivity.

5 channel states, typically without changes in ion selectivity.

6 region of both proteins alter the channel's ion selectivity.

7 7 A, consistent with a barrier mechanism for ion selectivity.

8 lular function with dynamic changes in their ion selectivity.

9 conclusions regarding the physical basis of ion selectivity.

10 NMR03 show good agreement with experimental ion selectivity.

11 pports existing hypotheses for mechanisms of ion selectivity.

12 18 (W568L) abolishes inactivation and alters ion selectivity.

13 the type of ligands are important factors in ion selectivity.

14 ns had normal barrier function but defective ion selectivity.

15 y with mole fraction due to the preferential ion selectivity.

16 res is explained by a theory of preferential ion selectivity.

17 additional dimension to tune the operational ion selectivity.

18 t must be placed on inferences about channel ion selectivity.

19 A have opposite sensitivity to pH and unique ion selectivity.

20 of H-L-H involvement in either pH gating or ion selectivity.

21 stringency and thermodynamic origin of metal-ion selectivity.

22 r intrinsic electrostatic properties control ion selectivity.

23 cally reduced single-channel conductance and ion selectivity.

24 ws efficient permeation without jeopardizing ion selectivity.

25 rent from Ca(2+)-induced changes, indicating ion selectivity.

26 nce of discrete channel or pore formation or ion selectivity.

27 y all mono- and divalent cations, showing no ion selectivity.

28 ackbone mutations do not significantly alter ion selectivity.

29 ensitivity to ATP, inward rectification, and ion selectivity.

30 r understanding the molecular basis of metal ion selectivity.

31 alphaAsp(602)) may have a role in conferring ion selectivity.

32 ng kinetics, single-channel conductance, and ion selectivity.

33 )](i) that mimic inactivation and changes in ion selectivity.

34 a2+ current inactivation, but did not affect ion selectivity.

35 tiple conductance states that have identical ion selectivity.

36 versal potential near -25 mV indicating poor ion selectivity.

37 raction surface, including those involved in ion selectivity.

38 flow (EOF), ion current, rectification, and ion selectivity.

39 ed redesign of channelrhodopsins for altered ion selectivity.

40 er region, yet exhibit drastically different ion selectivity.

41 mic coupling between CRAC channel gating and ion selectivity.

42 ted energetic and solvation contributions to ion selectivity.

43 alter the channel's unitary conductance and ion selectivity.

44 ow-conducting open state (O2) with differing ion selectivity.

45 e could contribute to altering face-specific ion selectivity.

46 inding with channel gating and modulation of ion selectivity.

47 BEST1 responsible for Ca(2+) activation and ion selectivity.

48 the transverse electric field and reversing ion selectivity.

49 is not a major contributor to the channel's ion selectivity.

50 nd closed in the light, without altering its ion selectivity.

51 rotransmission depending on their ligand and ion selectivity.

52 identical binding sites can exhibit diverse ion selectivities.

53 ues can compromise other properties, such as ion selectivity(21,22) or mechanical stability(23).

54 on suppression, and enhancement of coeluting ions, selectivity, accuracy, precision, and stability.

55 inactivation, open probability), permeation (ion selectivity, affinity for Ca(2+) block, La(3+) sensi

56 Their ion selectivity, amantadine sensitivity, specific activi

57 n of the G77A mutant revealed wild-type-like ion selectivity and apparent K(+)-binding affinity, in a

58 The structures allow the origins of metal ion selectivity and aspects of the molecular mechanism t

59 Cation channels with unitary conductance, ion selectivity and Ca2+-dependence similar to those of

60 ning functional features of ion channels are ion selectivity and channel gating.

61 lar loops as important structural motifs for ion selectivity and channel inhibition in Panx1.

62 ramework for understanding the mechanisms of ion selectivity and conductance by vertebrate CaV channe

63 (+) binding sites, thereby defining the high ion selectivity and controlling the transport rate of K(

64 asis for their voltage-dependent activation, ion selectivity and drug block is unknown.

65 inetics at near-threshold potentials altered ion selectivity and facilitated the conductance of both

66 tion on KV 2.1 function leading to a loss of ion selectivity and gain of a depolarizing inward cation

67 for Orai1 puncta formation, suggesting that ion selectivity and gating are mechanistically coupled i

68 The ion selectivity and gating behavior of the two conductan

69 els possess a highly conserved structure for ion selectivity and gating mechanisms.

70 tic mechanisms can be distinguished by their ion selectivity and inhibitor sensitivity.

71 mino acid substitutions of Subdued alter the ion selectivity and kinetic properties of the CaCC chann

72 ChR2 that could boost current while altering ion selectivity and observed that the mutations that red

73 locus), a principal determinant of inorganic ion selectivity and organic cation permeation.

74 uctural insights into possible mechanisms of ion selectivity and permeation for K+ channels.

75 alpha(1S)) muscle differ from one another in ion selectivity and permeation properties, including uni

76 ch ion pair interactions dramatically alters ion selectivity and permeation.

77 The MOD-1 channel has distinctive ion selectivity and pharmacological properties.

78 amental aspects of channel assembly, gating, ion selectivity and pharmacology remain obscure.

79 ihydropyridine (I(VDDR)) displayed kinetics, ion selectivity and pharmacology that differed from dihy

80 previously unrecognized residues involved in ion selectivity and photocurrent kinetics.

81 vely charged amino acid residue reverses the ion selectivity and produces chloride-conducting ChRs (C

82 ential component required for maintenance of ion selectivity and proper gating of Kv-type K+ channels

83 n of ASICs, illuminate the basis for dynamic ion selectivity and provide the blueprints for new thera

84 Additionally, the study explores ion selectivity and rectification based on the electric

85 n time of alpha7 nAChRs but had no effect on ion selectivity and relatively little, if any, effect on

86 nwardly rectifying K+ (GIRK) channels alters ion selectivity and reveals sensitivity to inhibition by

87 ility of a claudin to influence paracellular ion selectivity and support a role for the claudins in c

88 hich may be involved in metal sensing, metal ion selectivity and/or in regulation of the pump activit

89 ct from all known channels, predicting novel ion-selectivity and gating mechanisms.

90 onization (CDI) is currently limited by poor ion-selectivity and low salt adsorption capacity of poro

91 riable sensitivities to amiloride, different ion selectivities, and diverse unitary conductances.

92 ith their similar magnitudes of conductance, ion selectivities, and localization within eukaryotic ce

93 single-channel conductance, (4) a change in ion selectivity, and (5) a reduction in calcium pore blo

94 ure, pharmacology, activation, inactivation, ion selectivity, and arrhythmias.

95 uch as ion concentration polarization (ICP), ion selectivity, and conductance, are significantly affe

96 ithelial Na(+) channels (ENaC) (conductance, ion selectivity, and long mean open and closed times) wa

97 been constructed that possess pH-controlled ion selectivity, and membranes have been made from gold

98 hen mutated, affects unitary conductance and ion selectivity, and modulates pore block.

99 se coupling between ORAI1 channel gating and ion selectivity, and open a new avenue to dissect the ga

100 domain influence single channel conductance, ion selectivity, and other aspects of receptor function

101 ng sites are not exclusively responsible for ion selectivity, and other steps downstream in the mecha

102 ed ideas for voltage-gated channel function, ion selectivity, and pharmacology.

103 cluding loss of voltage sensitivity, loss of ion selectivity, and reduced cell-surface expression.

104 Current methods present low ion selectivity, and require multistep processes to tran

105 ies provide the structural basis for gating, ion selectivity, and single-channel conductance properti

106 res would display identical Ca(2+) affinity, ion selectivity, and unitary current magnitude.

107 The calculated conductance and ion selectivity are in good agreement with the experimen

108 ar requirements for gating and modulation of ion selectivity are similar, yet substantively different

109 ly, E190, a residue considered important for ion selectivity, are not close to the pore.

110 In this study, the concept of nontrivial ion selectivity arising in a highly flexible protein bin

111 Furthermore, Glu-90 is crucial to ion selectivity as also revealed by mutation of this res

112 tly to K+ and Na+ is a fundamental aspect of ion selectivity, as is the ability of multiple K+ ions t

113 (2)Si(CH(2))(3)NH(2) introduces pH-dependent ion selectivity at the pore orifice, a consequence of th

114 These results provide insights on external ion selectivity at the three binding sites.

115 The difference in ion selectivity between the wild-type and the mutants is

116 of apparent dominance not only of GluR2 for ion selectivity, but also of the flip isoform for recept

117 tics typical of a multi-ion pore and derives ion selectivity by Ca2+ binding.

118 Here, we explored the basis for ion selectivity by incorporating unnatural amino acids i

119 The origin of metal ion selectivity by members of the SmtB/ArsR family of ba

120 s rise to mechanosensitive channels in which ion selectivity can be altered by NOMPC mutation, indica

121 enetics, thus directed engineering to modify ion selectivity can be highly beneficial.

122 re, in P2X4 receptors, this ability to alter ion selectivity can be increased or decreased by alterin

123 Our results demonstrate that K+ ion selectivity can be retained even with significant re

124 ities that exceed seawater levels, and their ion selectivity can be tuned to configure them into swit

125 The weaker ion selectivity caused by IT led to hyperpolarizing shif

126 syn-photocycles now explain inactivation and ion selectivity changes of ChR2 during continuous illumi

127 IL-1beta treatment led to alterations in TJ ion selectivity, combined treatment of TNF-alpha and IFN

128 RyR1-G4934A had reduced K(+) conductance and ion selectivity compared with WT.

129 nd altered apical side tight junction sodium ion selectivity, compared with wild-type mice.

130 ween the Y and D positions, which determined ion selectivity, conductance and gating.

131 its function as a selectivity filter, affect ion selectivity, conductance, and open channel block.

132 that have distinct characteristics including ion selectivity, conductance, voltage dependence, and re

133 These results provide insights into ion selectivity, coupling and translocation, and establi

134 f side chains at 335 and 783 also results in ion selectivity defects, suggesting that the packing int

135 ervening hairpin segment that determines the ion selectivity (designated P).

136 ocurrent inactivation, and alteration of the ion selectivity during continuous illumination are not w

137 ltered the conductance (E207Q and D219N) and ion selectivity (E207Q) of the channel.

138 s phylogeny we infer ancestral states of the ion selectivity filter and show that this state has been

139 our wild-type Na(V)Ab models, reshaping the ion selectivity filter at the extracellular end of the p

140 to D112 in the transmembrane VSD to form the ion selectivity filter in the channel's open conformatio

141 f, GYGD, contributes to the formation of the ion selectivity filter in voltage-gated K+ channels and

142 to animal sodium channels and has a putative ion selectivity filter intermediate between calcium and

143 pecificity can be explained by the conserved ion selectivity filter observed in the channel's crystal

144 The multi-ion selectivity filter of our CaVAb model establishes a

145 permeation in the absence of activators: the ion selectivity filter on the external side of the pore

146 ely charged glutamate residues that form the ion selectivity filter with neutral glutamine or positiv

147 08 at the extracellular surface, T189 in the ion selectivity filter, and all phenylalanine residues.

148 e triplet of amino acids in the channel pore ion selectivity filter, and this sequence is different f

149 re, the P region, in addition to forming the ion selectivity filter, functions as the channel gate, t

150 onservation of sequence and structure of the ion selectivity filter, whereas the rates of K(+) turnov

151 the inner helix forms the presumed chloride ion selectivity filter.

152 forms an extracellular dome that shields the ion-selectivity filter from neurotoxin attack.

153 The gate and ion-selectivity filter of the P2X7R could be colocalized

154 predicted extracellular loop adjacent to the ion-selectivity filter of TRPC5.

155 electivity motif DEKA, line the walls of the ion-selectivity filter, whereas Glu and Lys are in posit

156 for other amino acids located within the CIC ion-selectivity filter.

157 ey demonstrate that cations can permeate the ion selectivity filters even when channels are closed.

158 Comparison of the ion selectivity filters toward the extracellular end of

159 t with the experimentally observed change in ion selectivity from cationic to anionic.

160 dependence of the wild type and had switched ion selectivity from cations to anions.

161 solute transporters, regulates KCNQ channel ion selectivity, gating, and pharmacology by direct phys

162 experimental single-channel conductance and ion selectivity (i.e., the reversal potential).

163 stibule does not significantly contribute to ion selectivity, implying that Ca(2+) selectivity is con

164 In this paper, disrupting the ion selectivity in another GIRK channel, chimera I1G1(M)

165 gands that control their thermodynamic metal ion selectivity in aqueous solution, and their use in se

166 lators and may be a dominant factor in metal ion selectivity in biology.

167 Ion selectivity in CLC-ck2 is similar to that in CLC-ec1

168 To reveal the structural mechanism of ion selectivity in CNG channels, particularly their Ca(2

169 the larger volume and mass, suggesting that ion selectivity in force-distance measurements are relat

170 We have investigated aspects of ion selectivity in K+ channels by functional expression

171 e barriers to ion conductance and origins of ion selectivity in models of the cationic human alpha7 n

172 into the molecular mechanisms that determine ion selectivity in OmpF porin.

173 ZnAF-2 zinc ion indicator provided high zinc ion selectivity in physiological solutions containing mi

174 Arcs provide an explanation for the observed ion selectivity in protegrin electrophysiology experimen

175 ted to clarify the molecular determinants of ion selectivity in protein binding sites.

176 rformed, and the major determinants of metal ion selectivity in proteins are not yet well understood.

177 K-1, TASK-1, and TASK-3 K(+) channels change ion selectivity in response to lowered pH(o), provide in

178 patch, we found that the channel shifted its ion selectivity in response to the change of intracellul

179 To understand the underlying principles of ion selectivity in tetrameric cation channels, we engine

180 t and hBest1, we find a sensitive control of ion selectivity in the bestrophins, including reversal o

181 he barriers to ion conduction and origins of ion selectivity in the GLIC channel by the construction

182 Gly-Met-Asn (GMN) motif, revealing clues of ion selectivity in this unique channel family.

183 mate residues (EEEE locus) are essential for ion selectivity in voltage-gated Ca(2+) channels, with i

184 Thus, metal ion selectivity in ZntA and possibly other P1-type ATPas

185 In studying ion-selectivity in biomaterials, it is common to study i

186 Moreover, analysis of ion selectivity indicated that the molecular requirement

187 annel catalytically inactive and altered the ion selectivity, indicating that the ion channel and the

188 Ion selectivity is a defining feature of a given ion cha

189 Our results indicate that ion selectivity is accomplished by the contribution of m

190 Our findings provide new insight into how ion selectivity is achieved in the paracellular pore.

191 suggest that the channel pore is widened and ion selectivity is altered by mutations at the G98 site

192 ic forces are dominant (rigid binding site), ion selectivity is controlled by the ion-ligand interact

193 Ion selectivity is critical for the biological functions

194 Ion selectivity is generally considered an immutable pro

195 Here, we show that TWIK1 ion selectivity is modulated by extracellular pH.

196 containing major structural determinants of ion selectivity is neighbored by wide vestibules on both

197 Ion selectivity is one of the basic properties that defi

198 In this case, ion selectivity is set by the interplay between ion-liga

199 The molecular basis for this metal ion selectivity is unclear.

200 One obvious mechanism of ion selectivity is when a binding site is structurally c

201 h of approximately 8 A; we estimate that the ion selectivity lies approximately 13 A below the outer

202 The ion selectivity locus comprises four glutamate residues

203 erant Drosophila species maintained their MT ion selectivity, maintained stable extracellular ion con

204 tecture of a tetrameric cation channel whose ion selectivity mechanism appears to be distinct from th

205 mpeting ions is the essential feature in the ion selectivity mechanism of voltage-gated Ca(2+) channe

206 tion, confirm fundamental predictions of the ion selectivity model, and further elucidate electrostat

207 The alkali metal ion selectivities of the 12 hexamers were evaluated by a

208 The ion selectivity of a membrane ion conductance that is in

209 ave investigated the NMR structure and metal ion selectivity of a natural finger of lower stability d

210 n that expected and (ii) the analogous metal ion selectivity of a zinc metalloenzyme (carbonic anhydr

211 We switched the ion selectivity of an inhibitory LGC-55 anion channel to

212 e glycine receptor (GlyR), revealed that the ion selectivity of anion channels is basically determine

213 The evolutionary implications for metal ion selectivity of ArsR/SmtB metal sensor proteins are d

214 Moreover, the ion selectivity of bBest2 is controlled by multiple resi

215 tation stabilizes channel opening and alters ion selectivity of Ca(v)1.4 in a manner that is strength

216 Ion selectivity of CFTR, ANO1 and GlyR is critically aff

217 Metal ion selectivity of Cpx1 expression is identical to that

218 Ion selectivity of four-domain voltage-gated Ca(2+) and

219 s during sorting could be minimized by using ion selectivity of hydrogel-infiltrated microbead membra

220 Differences in the kinetics and ion selectivity of hyperpolarization-activated currents

221 Our findings indicate that switches in ion selectivity of ligand-gated ion channels (LGICs) do

222 Few studies measuring thermodynamic metal ion selectivity of metalloproteins have been performed,

223 Here we show changes in the ion selectivity of neuronal P2X transmitter-gated cation

224 tage-independent manner without changing the ion selectivity of P2X(4) channels.

225 However, the structural basis for ion selectivity of paracellular pores is poorly understo

226 The high ion selectivity of potentiometric and optical sensors ba

227 The ion selectivity of pumps and channels is central to thei

228 Cellular stimuli can modulate the ion selectivity of some anion channels, such as CFTR, AN

229 entified a set of mutations that convert the ion selectivity of the 5-HT(3A) receptor from cationic t

230 ium transport to uptake of glutamate and the ion selectivity of the affinity for the transported amin

231 ge similar to that previously shown to alter ion selectivity of the bacterial sodium channel Na(V)Bh1

232 is unprecedented observation in terms of the ion selectivity of the binding sites in the membrane rot

233 cting a heterostructure with graphene oxide, ion selectivity of the BP membrane increases by ~80%, co

234 This suggests a possible dependence of the ion selectivity of the central pore on the folding topol

235 rane charges can control the conductance and ion selectivity of the CNT porins, thereby establishing

236 the predicted pore region of TRP-4 alter the ion selectivity of the conductance.

237 point mutation of Orai1 (E106D) altered the ion selectivity of the induced Ca(2+) release-activated

238 The ion selectivity of the large conductance channel is Br(-

239 Here we show that ion selectivity of the lysosomal ion channel TPC2, which

240 ids such as ouabain and digoxin switched the ion selectivity of the Na+ channel to this state of prom

241 current rectification phenomenon, a reversed ion selectivity of the nanopore occurs when the concentr

242 The ion selectivity of the rimantadine-sensitive current thr

243 t direction and was inversely related to the ion selectivity of the RyR pore.

244 channel is Br(-) > Cl(-) > I(-), whereas the ion selectivity of the small conductance channel is Br(-

245 ace charge potentials that contribute to the ion selectivity of this gap junction channel.

246 The difficulty of determining the ion selectivity of this minimalistic ion channel is due

247 opsins has illuminated mechanisms underlying ion selectivity of this remarkable family of light-activ

248 performed systematic characterization of the ion selectivity of TPC1 from Arabidopsis thaliana (AtTPC

249 y (ITC) studies revealed that the tailorable ion selectivity of U60 clusters is a result of the therm

250 Ca2+o-masked channels or from changes in the ion selectivity of voltage-gated Ca2+ or K+ channels.

251 1 side chain is not a primary determinant of ion selectivity or conduction in the wild-type channel,

252 binding, pH-induced conformational changes, ion selectivity or substrate specificity.

253 caused by changes in the voltage-dependence, ion selectivity, or apparent agonist affinity of the AMP

254 SMIT1 had no effect on Kv1.1 (KCNA1) gating, ion selectivity, or pharmacology.

255 We propose that the principle of ion selectivity outlined here may provide a rationale fo

256 into one that is promiscuous with respect to ion selectivity, permitting calcium ions (Ca2+) to perme

257 onductance that differed from MG channels in ion selectivity, pharmacology and sensitivity to connexi

258 ctional synapses whose properties (kinetics, ion selectivity, pharmacology, and ultrastructure) were

259 he voltage-gated potassium channels, but the ion selectivity pore domain sequence resembles that of a

260 Metal ion selectivity presumably comes from the coordination g

261 +-blockable cationic currents with different ion selectivity profiles that are carried by different c

262 Details of their underlying ion selectivity properties are still not fully understoo

263 conserved S1-S2 loop of Orai transforms the ion selectivity properties of CRAC current from being Ca

264 These changes in ion selectivity provide strong evidence that Orai1 is a

265 ) structures, key questions about gating and ion selectivity remain.

266 eceptor, it appears that the determinants of ion selectivity represent a conserved feature of the lig

267 reening length, consistent with the observed ion selectivity resulting from electrostatic interaction

268 These structures advance understanding of ion selectivity, reversed polarity gating, and cAMP regu

269 Without exception, SMIT1 altered KCNQ ion selectivity, sensitivity to extracellular K(+), and

270 -gated non-selective cation channel with the ion selectivity series lithium > sodium > N-methyl-d-glu

271 To test the effect on activity and metal ion selectivity, single alanine, histidine, and serine s

272 Metal-ion selectivity studies demonstrate that CP prefers to c

273 on concentration and species, and it altered ion selectivity, suggestive of pore constriction.

274 The dynamic change of ion selectivity suggests a charge-screening mechanism fo

275 ore were more likely to affect gating and/or ion selectivity than those in the upper pore.

276 terminants of single channel conductance and ion selectivity that are not associated with the TM2 dom

277 ide detailed information about mechanisms of ion selectivity that is missing from mechanisms derived

278 roperties, such as electrical resistance and ion selectivity that would complement known differences

279 ssing wild-type TRPV1, indicative of loss of ion selectivity, that was completely absent in cells exp

280 ty filter mutant constructed to have altered ion selectivities, the sodium ion binding site nearest t

281 For ZntA homologues with different metal ion selectivity, the cysteines are replaced by serine, h

282 Although most ion channels exhibit stable ion selectivity, the prevailing view of purinergic P2X r

283 However, the key questions-such as, the ion selectivity, the transport pathway, and the gating m

284 common protein structure but differ in their ion selectivity, their affinity for the blocker amilorid

285 how that the D position is able to fine-tune ion selectivity through a functional interaction with th

286 , inhibited Ic by 80 % without affecting its ion selectivity, thus confirming and extending the recen

287 The mutated GIRK1 and GIRK2 retained ion selectivity to K(+) ions.

288 We determined metal-ion selectivity under fixed conditions using the voltage

289 its contributes one pore loop to the central ion selectivity unit at the interface between the subuni

290 Control of ion selectivity via ATI is proposed to be a natural, epi

291 MCC activity with the same conductance, ion selectivity, voltage dependence, and peptide sensiti

292 were unaltered in R1389H channels including ion selectivity, voltage-dependent activation or voltage

293 Ion selectivity was found to be essentially the same for

294 For both topologies, the same ion selectivity was found with a preference for K(+) fol

295 These changes in ion selectivity were confirmed by cation-dependent ATP h

296 tation, along with unexpected alterations in ion selectivity, were generally larger in channels lacki

297 Mutating Trp74 or the nearby Arg75 disrupt ion selectivity, whereas altering residues in the hydrop

298 of dominance of GluR1(R) in determination of ion selectivity, whereas expression of GluR1(R) flip wit

299 CDI cells have demonstrated size-based ion selectivity wherein smaller hydrated ions are prefer

300 the open-state transitions and the discrete ion selectivity within these states.