ライフサイエンスコーパス: isolectin

1 tes increases with increasing valency of the isolectin.

2 high specificity for the xenograft antigen, isolectin 1-B(4) from Griffonia simplicifolia (GS-1-B(4)

3 ional lattice is formed from fucose-specific isolectin A from Lotus tetragonolobus cross-linked with

4 ng the gene encoding for the germ agglutinin isolectin A protein (Tri a 18 allergen), using the fluor

5 The GSL I-B4 isolectin, a marker of early endothelial cells, specific

6 d B subunits and constitute a series of five isolectins (A4, A3B, A2B2, AB3, B4).

7 The vasculature was dual stained with isolectin and collagen IV.

8 in the rat forebrain was characterized by B4 isolectin and Fluoro-Jade labeling techniques after 4 do

9 lial markers, as determined by staining with isolectin and for the endothelial-specific protein plate

10 At P17, retinal flatmounts were stained with isolectin and quantified with a standard protocol to mea

11 n Matrigel culture, followed by formation of isolectin and von Willebrand Factor-expressing cells, si

12 eriments carried out using 125I-labeled GS-I isolectins and type A human erythrocytes allowed us to d

13 y expressed by sensory neurons that bind the isolectin B(4) (IB(4)).

14 Isolectin B(4)-positive [IB(4)(+)] primary afferent noci

15 populations, which were visualized using the isolectin B-4 and thiamine monophosphatase, were severel

16 jugates of cholera toxin B subunit (CTB) and isolectin-B(4) of Bandeiraea simplicifolia (IB(4)), were

17 00 (29%), but only to a moderate extent with isolectin B4 (16%).

18 DRG coexpressed Nav1.8 mRNA (84%) and bound isolectin B4 (72%), relatively few GFP profiles were pos

19 ous injection of the Griffonia simplicifolia isolectin B4 (IB4) at 1-28 days following a moderate tho

20 by cell size, action potential duration and isolectin B4 (IB4) binding] generated distinct responses

21 subunit B (CTB) for myelinated A fibers and isolectin B4 (IB4) for unmyelinated C fibers and both la

22 receptor potential vanilloid 1 (TRPV1), and isolectin B4 (IB4) from Griffonia simplicifolia.

23 marker recognized by Griffonia simplicifolia isolectin B4 (IB4) in the cephalic mesenchyme.

24 e injected cholera toxin B subunit (CTb) and isolectin B4 (IB4) into the vagus nerve.

25 Intravenous isolectin B4 (IB4) labels a subpopulation of blood vesse

26 erve growth factor dependent, binding either isolectin B4 (IB4) or antibodies to calcitonin gene-rela

27 erivascular markers such as Evans blue (EB), isolectin B4 (IB4) or laminin (LN) are used alongside si

28 ified afferents revealed that most CPMs were isolectin B4 (IB4) positive and transient receptor poten

29 e tomography (OCT), and vascular change with Isolectin B4 (IB4) postmortem staining.

30 beled with cholera toxin subunit B (CTB) and Isolectin B4 (IB4) respectively, and found that they exp

31 Double labeling with isolectin B4 (IB4) showed that all P2X3-ir neurons also

32 The isolectin B4 (IB4) stains a subset of small and medium-s

33 ated peptide (CGRP) or that bound the lectin isolectin B4 (IB4) was determined.

34 xclusively expressed in afferents binding to isolectin B4 (IB4), a neurochemical marker of non-peptid

35 In contrast, preventing sensory input from isolectin B4 (IB4)-binding fibers blocked movement-induc

36 Ablation of isolectin B4 (IB4)-binding, but not TRPV1(+), sensory af

37 potential (AP) rheobase, was found in weakly isolectin B4 (IB4)-positive and IB4-negative (IB4-) neur

38 sistant (TTX-R) Na+ channel currents between isolectin B4 (IB4)-positive and IB4-negative small DRG n

39 r calcitonin gene-related protein (CGRP) and isolectin B4 (IB4)-positive neurons in the L4 and L5 ips

40 We also show that isolectin B4 (IB4)-positive neurons, many of which are r

41 nces identified in non-peptidergic [strongly isolectin B4 (IB4)-positive] and peptidergic [weakly IB4

42 Treatment with isolectin B4 (IB4)-saporin or SSP-saporin (which deplete

43 osine kinase receptor Ret and bind the plant isolectin B4 (IB4).

44 n gene-related peptide (CGRP), or labeled by isolectin B4 (IB4).

45 sensory afferents that bind the plant lectin isolectin B4 (IB4).

46 cluding concanavalin A (conA), Vicia villosa isolectin B4 (VVL-B(4)), and Ricinus communis agglutinin

47 increased percentage of GFRalpha3, TRPV1 and isolectin B4 afferents in ART-OE mice.

48 ncta immunopositive for GluR2/4 costain with isolectin B4 after injections of these tracers in the sc

49 rons that appear after inflammation also had isolectin B4 binding, suggesting that some mechanorecept

50 hy, and CNV lesion size was quantified after isolectin B4 endothelial cell staining.

51 ology, and quantitative confocal analysis of isolectin B4 histochemistry on days 7 and 14.

52 predominantly colabeled with peripherin and isolectin B4 markers of unmyelinated C-fiber neurons; 68

53 change the primary afferent terminal markers isolectin B4 or calcitonin-gene-related peptide immunore

54 in-eosin, Masson trichrome, and biotinylated isolectin B4 stains were used to assess regional vascula

55 ome and Biotinylated Bandeiria simplicifolia Isolectin B4 stains were used to characterize scarred my

56 ly, a greater percentage of TL neurons bound isolectin B4 than LS neurons (LS, 61%; TL, 85%).

57 entin to identify Muller cells, and with the isolectin B4 to identify microglia and macrophages.

58 by histology and retinal mounts stained with isolectin B4 to quantify aberrant angiogenesis.

59 hese airspace abnormalities included reduced isolectin B4(+) alveolar capillaries and surfactant prot

60 The density of isolectin B4(+) vessels in peri-infarct cortex was simil

61 e number of double-positive cells (beta-gal, isolectin B4) on the luminal surface in carotid arteries

62 Utilizing isolectin B4+ neurons, the absence of KNa current result

63 ccurred in ATP responses in nAChR-expressing isolectin B4+ nonpeptidergic neurons 1 d postinjury, whe

64 bone marrow origin and Tie-2 expression, and isolectin B4, also indicating endothelial lineage, in th

65 The majority of EGFP-positive neurons bound isolectin B4, although a small percentage ( approximatel

66 I (BS-I), the wholly alpha-gal-specific BS-I isolectin B4, and elicited primate anti-pig xenoreactive

67 led with calcitonin gene-related peptide and isolectin B4, and injured unmyelinated afferents labeled

68 with expression of the surface markers LA4, isolectin B4, and LD2, kainate receptors are present on

69 of primary afferent nociceptors, peripherin, isolectin B4, and substance P, and markers of myelinated

70 ize with calcitonin gene-related peptide and isolectin B4, and thus represent nociceptors.

71 tmounts was evaluated by immunostaining with isolectin B4, and vascular permeability was analyzed by

72 ained for calcitonin gene-related peptide or isolectin B4, but none of these was immunopositive for s

73 c DRG cells with ADPs stained positively for isolectin B4, but only diabetic cells responded robustly

74 ession of Tek receptor tyrosine kinase(Tek), isolectin B4, endothelial nitric oxide synthase(eNOS), v

75 tem examination, tissue samples stained with isolectin B4, Masson trichrome, and hematoxylin-eosin we

76 ciceptive axons but had no effect on injured isolectin B4-binding nociceptive axons.

77 size of subretinal scars, and the number of isolectin B4-labeled cells.

78 ing AMPH exposure was quantified by counting isolectin B4-labeled phagocytic microglia and Fluoro-Jad

79 OIH produced by systemic LDM is mediated by isolectin B4-positive (IB4(+)) and peptidergic nocicepto

80 This signaling pathway was specific to isolectin B4-positive [IB4(+)] nociceptors.

81 in males; and (6) lack of dependence on the isolectin B4-positive nociceptor.

82 rize a novel subpopulation of capsaicin- and isolectin B4-positive nociceptors that also expresses a

83 bset of small-diameter nociceptors that bind isolectin B4.

84 peptide and neurokinins and those that bound isolectin B4.

85 nsitive and/or bound the nociceptive marker, isolectin B4.

86 area-and some co-labeled with substance P or isolectin B4.

87 ene-related peptide (CGRP), substance P, and isolectin B4.

88 phin with CGRP and substance P, but not with isolectin B4.

89 dergic neuron marker griffonia simplicifolia isolectin B4.

90 yelinated afferents (Griffonia simplicifolia isolectin B4; IB4).

91 Isolectin-B4 (IB4) binding was detected in a higher perc

92 ide (CGRP) for peptidergic terminals, and by Isolectin-B4 (IB4) for nonpeptidergic terminals.

93 d with fluorescein isothiocyanate-conjugated isolectin-B4 for endothelium to demarcate vascular struc

94 graphy (FA), confocal volumetric analysis of isolectin-B4 labeled flatmounts, and histologic examinat

95 vascular patterns consistent with ischemia; isolectin-B4 labeling revealed a capillary-free zone cen

96 rescence microscopy to detect blood vessels (isolectin-B4), angiogenesis [anti-vascular endothelial g

97 scence microscopy to visualize vessels using isolectin-B4, to detect angiogenesis using anti-VEGF and

98 d-rehydrated animals (R21)] and stained with Isolectin-B4-HRP to visualize microglial cells.

99 orm cortex of the younger rats, extensive B4 isolectin binding to activated microglia was observed in

100 y on the association constants of these GS-I isolectins binding to cells.

101 and (2) the association constant of the GS-I isolectins for human type A erythrocytes increases with

102 the increased affinity displayed by the GS-I isolectins for human type A erythrocytes is dependent on

103 MS sequencing confirmed the presence of two isolectin forms in commercially available WFA differing

104 y means of lectin histochemistry with the B4-isolectin from Griffonia simplicifolia as well as immuno

105 putative markers of nociceptive DRG neurons, isolectin Griffonia simplicifolia (I-B4), identifying sm

106 as were isolated, blood vessels stained with isolectin Griffonia simplicifolia, images of retinal who

107 en IV immunohistochemistry and staining with isolectin Griffonia simplicifolia.

108 f astrocytes, (b) griffonia simplicifilia B4-isolectin (GSA-IB4) horse radish peroxidase (HRP)-conjug

109 The Griffonia simplicifolia-I (GS-I) isolectins have been used to probe the effect of lectin

110 Microglia were labeled with GSA-IB4 isolectin HRP-conjugate, but they did not have NADPH-d a

111 Isolectin I-B(4) labeling for microglia/macrophages show

112 nst macrophages) and Griffonia simplicifolia isolectin IB(4), and stain intensely for the lysosomal p

113 RPE/choroid flatmounts were labeled with isolectin IB4 to determine CNV lesion volumes using conf

114 -diamino-2-phenylindole), endothelial cells (isolectin IB4), microglia (CD11b), and filamentous actin

115 SP), somatostatin (SOM) immunoreactivity and isolectin IB4, and cholera toxin B (ChTB) binding were e

116 s nociceptors by virtue of their binding the isolectin IB4.

117 ntified by neurofilament H-chain 200, I-B(4) isolectin (IB4), or tropomyosin receptor kinase A expres

118 ions of choroid/RPE flat-mounts labeled with isolectin-Ib4.

119 ions were reacted for activated microglia by isolectin immunochemistry.

120 Whole mounts of retinas were prepared for isolectin immunohistochemistry, and preretinal or intrav

121 This series of isolectins is tetravalent (A4), trivalent (A3B), divalen

122 By 7 days, a well-defined isolectin-labeled CNV network was present, and its volum

123 ser injury, phalloidin-labeled RPE cells and isolectin-labeled endothelial cells increased significan

124 Codistribution of Galphat-S-ir fibers and isolectin-labeled extrabulbar primary olfactory fibers w

125 enesis was performed using barium sulfate or isolectin microangiography and Doppler ultrasonography o

126 ate that (1) the association constant of the isolectin monovalent for alpha-D-GalNAc (AB3) is virtual

127 njugates of choleragenoid (B-HRP) and the B4 isolectin of Bandeiraea simplicifolia (IB4-HRP) on oppos

128 By day 4, isolectin-positive cells forming vascular tubes were vis

129 tran; immunohistochemistry with biotinylated isolectin, rabbit anti-NG2, rat anti-CD31, rabbit anti-V

130 Isolectin-stained retinal flat-mounts were analyzed for

131 re calculated from phosphohistone- and Alexa-isolectin-stained retinal flatmounts.

132 on in the mouse OIR model based on images of isolectin-stained retinal wholemounts, we were able to a

133 perfusion and elastin immunohistochemistry, isolectin staining, and confocal fluorescence microscopy

134 th 20 microg/ml Griffonia simplicifolia (GS) isolectin to identify microglia and define their morphol