ライフサイエンスコーパス: joining of

1 pies of its enantiomer through the templated joining of 11 component oligonucleotides.

2 Additional mechanisms can restrict joining of 12/23 RS matched segments beyond the 12/23 ru

3 We examined the joining of 3'-protruding single strand (PSS) ends, which

4 omal subunit in 48S initiation complexes and joining of 40S and 60S subunits.

5 a 60S ribosomal subunit protein required for joining of 40S and 60S subunits.

6 Joining of 48S complexes to 60S subunits to form 80S rib

7 The joining of a durable PD-1 blockage significantly boosted

8 the NHEJ-dependent and the NHEJ-independent joining of a signal end to a coding end.

9 s mutation has a dominant-negative effect on joining of a subset of DNA ends in an in vitro NHEJ assa

10 e and modern molecular biology, catalyze the joining of adjacent 3'-OH and 5'-phosphorylated ends in

11 events of joining of exons, and 76 events of joining of adjacent genes as a single gene.

12 unction is required for orientation-specific joining of AID-initiated DSBs.

13 D-12RSSs and, thereby, to mediate deletional joining of all D segments except RC-based DQ52, which jo

14 The synthesis involves convergent joining of an Eastern half and a Western half.

15 ic parabiosis, a surgical technique in which joining of animals of different ages leads to a shared c

16 ar efficiency, but addition of Ku suppresses joining of blunt ends and homologous ends with 3' overha

17 The liquid-solid joining of BMGs can shed more insights on overcoming the

18 the transpososome actively promotes coupled joining of both ends of the element DNA into the same ta

19 XLF (Cernunnos) stimulates the joining of both incompatible DNA ends and compatible DNA

20 Here we show that joining of both matched and mismatched DNA ends occurs e

21 y > 50 microM 3-aminobenzamide prevented the joining of both matched and non-homologous DNA ends.

22 e sites is coupled such that the coordinated joining of both Mu DNA ends is favored during recombinat

23 he two Ku molecules, which suggests that the joining of both strands of a DSB involves both LigIV mol

24 is more stable, consistent with the covalent joining of both strands of the duplex and a lower overal

25 The joining of breaks in the DNA phosphodiester backbone is

26 has been suggested to occur by nonhomologous joining of broken DNA ends.

27 into two basic operations: DNA cleavage and joining of broken ends.

28 activation chemistry could have enabled the joining of building blocks in aqueous solution from a co

29 efect in Brca1 mutant cells but prevents the joining of chromatid breaks into chromosome rearrangemen

30 NHEJ repair, thus preventing deletional end-joining of chromosomal DSBs during G1.

31 ce of any known NHEJ factor severely impairs joining of cleaved V, D, and J segments.

32 Unexpectedly, trans joining of coding ends is severely impaired (100-to 1000

33 gements are initiated normally, but impaired joining of coding ends prevents assembly of functional r

34 HEJ-dependent, and not the NHEJ-independent, joining of coding ends to signal ends.

35 also needed in the later steps for efficient joining of coding ends.

36 ll extracts for the ability to stimulate the joining of cohesive DNA ends in a complementation assay

37 ally complementary overhangs, and stimulated joining of cohesive ends more than twentyfold.

38 ese proteins alone did not promote efficient joining of cohesive-ended DNA fragments in a cell-free a

39 itol hexakisphosphate (IP(6)) stimulates the joining of complementary DNA ends in a cell free system.

40 generation sequencing, de novo assembly, and joining of contigs by Sanger sequencing.

41 erly degenerate, which can physically hinder joining of contralateral heart cells leading to a broken

42 P act in the same pathway as LIG3 to promote joining of de-protected telomeres by A-NHEJ.

43 The joining of different genomes in allotetraploids played a

44 re consistent with a mechanism of cutting or joining of distal DNA lesions initiated by activation-in

45 interactions with, and robust V(H)-to-DJ(H) joining of, distal V(H)s, with patterns similar to those

46 nd breaks promotes non-homologous end-to-end joining of DNA [3].

47 he end-joining reaction was in progress, end-joining of DNA already present in the reaction continued

48 mology-directed repair and nonhomologous end joining of DNA breaks is impaired in KDM4D-deficient cel

49 ntaining genomic stability during long-range joining of DNA breaks.

50 luding translocations, require formation and joining of DNA double strand breaks (DSBs).

51 genes are also involved in nonhomologous end joining of DNA double-strand break repair, the largest s

52 sponsible for PG removal during in vitro end joining of DNA double-strand breaks (DSBs), whole-cell e

53 The accurate joining of DNA double-strand breaks by homologous recomb

54 nation mediated by Cre or non-homologous end joining of DNA double-strand breaks induced by I-Sce1, r

55 ir proteins that catalyze non-homologous end-joining of DNA double-strand breaks.

56 s) that is involved in the nonhomologous end joining of DNA double-strand breaks.

57 etic drugs are thought to arise by incorrect joining of DNA double-strand breaks.

58 counteracting error-prone non-homologous end joining of DNA double-strand breaks.

59 We conclude that joining of DNA ends during CSR does not require DNA-PKcs

60 The joining of DNA ends during Ig class-switch recombination

61 e translocations frequently form through the joining of DNA ends from double-strand breaks (DSBs) gen

62 onal chromosomal locations to facilitate the joining of DNA ends generated by other types of DSBs.

63 0 are known to be required for nonhomologous joining of DNA ends in vivo.

64 These extracts effected little joining of DNA ends with cohesive 5' or 3' overhangs, an

65 us DNA by opening chromatin and facilitating joining of DNA ends.

66 t the final steps of V(D)J recombination and joining of DNA ends.

67 op a ligase-free technology for the covalent joining of DNA fragments to suitable plasmid vectors.

68 gene that is essential for nonhomologous end joining of DNA in double-strand break repair.

69 DNA replication and repair by catalyzing the joining of DNA nicks.

70 DNA ligases catalyse the joining of DNA single- and double-strand breaks.

71 topoisomerase catalyzes the cleavage and re-joining of DNA strands through a DNA-(3'-phosphotyrosyl)

72 DNA ligases catalyze the joining of DNA strands to complete DNA replication, reco

73 Notably, the joining of DNA substrates that require the combined acti

74 -PKcs (DNA-PK) promotes the synapsis and the joining of double strand breaks (DSBs) during canonical

75 nd with its known role in non-homologous end joining of double strand breaks, perhaps including those

76 n is initiated by microhomology-mediated end joining of double strand breaks.

77 End joining of double-strand breaks (DSBs) requires Ku prote

78 surveyed the role of NHEJ or SSA factors in joining of double-strand breaks (DSBs) with no complemen

79 Thus the joining of double-strand breaks by mammalian extracts is

80 se Ku is required for accurate and efficient joining of double-strand breaks while similar DNA repair

81 es in V(D)J recombination, nonhomologous end-joining of double-strand breaks, and regulation of the D

82 ficiently participates in non-homologous end joining of double-strand DNA breaks.

83 is also demonstrated that Zn(2+) blocks end-joining of double-stranded (ds) DNA fragments with 3' ov

84 s no significant effect on nonhomologous end-joining of DSB ends, but it causes a significant reducti

85 DNA, has been observed in the non-homologous joining of DSB ends.

86 nduced cytidine deaminase (AID) and involves joining of DSB intermediates by end joining.

87 r DNA-PK, the initiator of nonhomologous end-joining of DSB, was involved in repair of CNDAC-induced

88 (ATM) kinase, which suppresses illegitimate joining of DSBs and activates cell-cycle checkpoints.

89 clear cells from FLT3/ITD knock-in mice, end-joining of DSBs occurs at microhomologous sequences resu

90 fecting either IgH germline transcription or joining of DSBs within S regions by classical nonhomolog

91 class switching that involves generation and joining of DSBs within two different downstream S region

92 uces phosphorylation of H2A, processing, and joining of DSBs.

93 , we show that BS cells display aberrant end-joining of DSBs.

94 mpairs Ku80 removal after non-homologous end joining of DSBs.

95 over, the Zn-f stimulates intermolecular end joining of duplexes that harbour these structures up to

96 ctions that involve distant sites, including joining of dysfunctional telomeres and AID (also known a

97 at the excursions promote non-homologous end joining of dysfunctional telomeres and implicated Nespri

98 ontrolled resection of DNA ends, impairs end-joining of dysfunctional telomeres, and abrogates class

99 is required for efficient non-homologous end-joining of dysfunctional telomeres.

100 RtcB catalyzes the GTP and Mn(II)-dependent joining of either 2',3'-cyclic phosphate or 3'-phosphate

101 anner identical to other Ku's and stimulated joining of ends by the host NHEJ DNA ligase (LigD).

102 Here we find that the joining of ends by XRCC4-ligase IV is markedly influence

103 mutational event in which deletions form via joining of ends from two closely spaced DSBs (double cut

104 Joining of ends requiring gap filling prior to ligation

105 Consistent with a mechanism involving joining of exchanged double-strand break ends, there was

106 nformation to the physical map and result in joining of existing physical map contigs into 84 cluster

107 recting the physical excision of introns and joining of exons have been elucidated in recent years, t

108 19 novel translational frames, 28 events of joining of exons, and 76 events of joining of adjacent g

109 mechanisms must exist to prevent the random joining of exons.

110 The joining of exposed 3'transposon ends to the target DNA c

111 e-specific recombinase involves breakage and joining of four DNA strands between two target substrate

112 cer-prone BALB/c mice showed inefficient end joining of gamma ray-induced DSBs as compared with cells

113 ents, random nucleotide incorporation during joining of gene segments into a complete transcript, and

114 to its ability to engage in promiscuous end joining of genomic instability and also leads to increas

115 that polymerize into filaments by end-to-end joining of hetero-oligomeric complexes.

116 air, RecA family proteins must promote rapid joining of homologous DNA.

117 to IgH V(D)J rearrangement to facilitate the joining of Ig variable, diversity, and joining segments.

118 Reduction of PALF in vivo reduces the joining of incompatible DNA ends.

119 also participate in imprecise rejoining and joining of incompatible ends, important mutagenic events

120 f radiation-induced cell killing, reflecting joining of incongruent DNA-ends that alter the genome.

121 tor 5B (eIF5B) on the 80S ribosome after the joining of individual ribosomal subunits-a process that

122 53BP1 facilitates joining of intrachromosomal DSBs but only at distances c

123 consisting of 83 nucleotides, catalyses the joining of L-mono- or oligonucleotide substrates on a co

124 translation factor eIF5B, a GTPase, promotes joining of large (60S) subunits with pre-40S subunits to

125 bosomal subunit protein that is required for joining of large and small ribosomal subunits.

126 uses a highly conserved sequence (CS) in the joining of leader and body RNAs.

127 Covalent joining of leader RNA exons to pre-mRNAs by trans-splici

128 ereas polymerization is achieved by covalent joining of lysine side chains within distinct alanine-ri

129 he context of deterministic transferring and joining of materials at the microscale where surface adh

130 : filament nucleation, growth of microvilli, joining of microvillar bundles into modules, assembly of

131 sequence analysis of junctions derived from joining of mismatched DNA ends in XRCC4-deficient cells

132 complex is necessary for resection-based end joining of mismatched DNA ends.

133 .g. Leica Bond), 40x-resolution imaging, and joining of multiple patients' tissue sections per captur

134 n through double strand breaks (DSB) and the joining of newly excised transposon ends with target DNA

135 This paper is investigating the joining of nodes in a loose lattice structure by deliver

136 sphorylation provides one route by which end-joining of non-homologous DNA can be regulated.

137 ase's endonuclease activity in promoting the joining of noncompatible ends.

138 e ligation step plays a critical role in the joining of noncomplementary DNA ends during nonhomologou

139 nction with a DNA ligase, Mre11 promotes the joining of noncomplementary ends in vitro by utilizing s

140 and Xenopus eggs possess activities for the joining of nonhomologous DNA ends, and such activities m

141 The synthesis, processing, and joining of Okazaki fragments during DNA replication is c

142 iding clamp, co-ordinates the processing and joining of Okazaki fragments during eukaryotic DNA repli

143 gase I/PCNA interaction and suggest that the joining of Okazaki fragments is coordinated by pairwise

144 gase I to replication foci and the efficient joining of Okazaki fragments is dependent on the interac

145 Flap endonuclease 1 (FEN1), involved in the joining of Okazaki fragments, has been proposed to restr

146 NA ligase I that complete the processing and joining of Okazaki fragments, respectively.

147 in-protein interface that may coordinate the joining of Okazaki fragments.

148 ts that other factors may be involved in the joining of Okazaki fragments.

149 e one active site promotes both cleavage and joining of one Mu DNA end.

150 sposon end: cleavage of the two strands plus joining of one strand to target DNA.

151 greater complexity, subsequent hierarchical joining of origami can be pursued.

152 We report here that end joining of P-element breaks in the absence of DmRad51 do

153 rnunnos protein restored gap filling and end joining of partially complementary overhangs, and stimul

154 ck, strong, and energetically less-intensive joining of polymeric glasses across various sectors.

155 olves separate transcription of gene pieces, joining of precursor RNAs via trans-splicing, and RNA ed

156 tive forms that lead to correct or incorrect joining of products.

157 nation, MRN deficiency leads to the aberrant joining of Rag DSBs and to the accumulation of unrepaire

158 ficient G1-arrested progenitor B cell lines, joining of RAG-generated DSBs in Ku70-deficient and Ku70

159 proach relies on the ordering, trimming, and joining of randomly cleaved parental DNA fragments annea

160 such as iterative recalibration and neighbor joining of reads to identify enriched regions of any len

161 st 2 decades have revealed that the aberrant joining of replication-associated breaks leads to catast

162 omosome stability by promoting efficient end joining of replication-derived breaks, as well as TLS an

163 ation of these pathways using as a model end joining of restriction endonuclease linearized plasmid D

164 vitro, extracts of 180BR cells supported end joining of restriction endonuclease-digested plasmid to

165 on of the GTPase activity of eIF5B after the joining of ribosomal subunits prevented the dissociation

166 An RNA ligase ribozyme that catalyzes the joining of RNA molecules of the opposite chiral handedne

167 RNA enzyme that catalyzes the RNA-templated joining of RNA was converted to a format whereby two enz

168 These proteins are important for faithful joining of S regions, and in their absence aberrant reco

169 bulins and T-cell receptors by combinatorial joining of segments of coding DNA.

170 The corner hole is formed from the joining of several five-member rings.

171 Here, we show that both trans cleavage and joining of signal ends occur efficiently in vivo.

172 nd Dnl4-Lif1 is much more efficient than the joining of similar DNA substrates that require only liga

173 DNA ligases catalyse the joining of single and double-strand DNA breaks, which is

174 ein in vitro have supported mainly uncoupled joining of single cDNA ends.

175 These properties may enable joining of single domains into multidomain proteins, lip

176 Moreover, joining of SmaI-generated blunt ends is generally imprec

177 alysis of the switch fragments showed direct joining of Smu and Sgamma3 without deletions or duplicat

178 ands A or G and that disulfide bond-mediated joining of strand A to the core strand B cooperatively s

179 DNA ligases catalyze the joining of strand breaks in the phosphodiester backbone

180 This may include non-homologous end-joining of strand breaks resulting from DNA damage, beca

181 iation factor IF2 (re and, like it, mediates joining of subunits and has a ribosome-dependent GTPase

182 reaks and, consistent with this result, that joining of such breaks is specifically decreased in cell

183 acting NHEJ and a slow-acting alterative end joining of switch region breaks during CSR.

184 on to mediate the synapsis, breakage and end joining of switch regions flanking Igh constant region e

185 Cloning DNA typically involves the joining of target DNAs with vector constructs by enzymat

186 shorten telomeric DNA, and nonhomologous end-joining of telomeric DNA, can lead to loss of telomere f

187 ' end of the viral DNA; strand transfer, the joining of the 3' ends of viral DNA to host DNA; and 5'-

188 ve site in TnsB also mediates the subsequent joining of the 3' ends to the target.

189 DNA; and 5'-end joining (or gap repair), the joining of the 5' ends of viral DNA to host DNA.

190 l translation involves the tightly regulated joining of the 50S ribosomal subunit to an initiator tra

191 hydrolysis of bound GTP with the concomitant joining of the 60 S ribosomal subunit to the 40 S initia

192 ctor 5B (eIF5B) is a GTPase that facilitates joining of the 60 S ribosomal subunit to the 40 S riboso

193 tion of the start codon, which culminates in joining of the 60S large subunit and formation of the 80

194 f the 48S preinitiation complex, followed by joining of the 60S ribosomal subunit and formation of th

195 A second GTPase, eIF5B, catalyzes the joining of the 60S subunit to produce an 80S initiation

196 ion to the site for editing does not require joining of the amino acid to the nucleic acid.

197 that these phenomena may reflect defects in joining of the broken DNA ends or in protection of the e

198 The later steps involve processing and joining of the cleaved DNA ends, and until now have been

199 Joining of the donor DNA to the acceptor DNA is detected

200 trong promoter for expression of mooV by the joining of the ends of the insertion sequence element at

201 ole in completion by promoting resection and joining of the excess DNA created when replisomes conver

202 end of Tn7 and target insertion results from joining of the exposed 3' Tn7 tips to the target DNA.

203 s/Delta)(TM) splice variant results from the joining of the first and third exons with skipping of th

204 om a protein, accompanied by the concomitant joining of the flanking polypeptide sequences, the extei

205 eir excision from precursor proteins and the joining of the flanking protein sequences (exteins).

206 on from a precursor protein with concomitant joining of the flanking sequences.

207 Joining of the fragments by DNA ligase I to generate the

208 the mouse SCID mutation in DNA-PKCS disrupts joining of the hairpin coding ends but spares joining of

209 generated in large part by the combinatorial joining of the Ig gene segments, VH, D, and JH, that tog

210 Upon joining of the large and small ribosomal subunits, a 100

211 ryotic translational initiation involves the joining of the large and small subunits of the ribosome,

212 Joining of the large, 50S, ribosomal subunit to the smal

213 shock momentum is important for rupture and joining of the LMNPs, providing much better control than

214 nucleotide in comparison to an efficient end joining of the nick substrate with a 3'-ribonucleotide b

215 d to mutagenic ligation or non-mutagenic end joining of the nick substrates.

216 oining of the hairpin coding ends but spares joining of the open signal ends.

217 Subsequent joining of the overhand knot end groups by ring-closing

218 ends of viral DNA, and sequence-independent joining of the recessed viral ends to staggered phosphat

219 Spatially coherent break-up leads to the joining of the spheres into a bispherical silicon 'p-n m

220 subsequent processing and LigIIIa-catalyzed joining of the SSB.

221 ble-strand break repair, and likely promotes joining of the strands of the convergent replication for

222 nitiator RNA primer that is removed prior to joining of the strands.

223 ts, redox co-factor acquisition, and correct joining of the subunits to form functional complexes.

224 We characterized the abnormal joining of the telencephalon to the diencephalon and def

225 inked by a protein bridge, since coordinated joining of the two ends is not disrupted by cleaving the

226 egration of retroviral cDNA involves coupled joining of the two ends of the viral genome at precisely

227 egion suggests a key role in stabilizing the joining of the two helical domains, and in maintaining t

228 e-strand break could be healed by (i) direct joining of the two partial genomes resulting from the br

229 both adenylation of the 5'-probe strand and joining of the two probe strands.

230 The joining of the two viral DNA ends to target DNA occurs s

231 The final joining of these blunt ends followed the same kinetics a

232 end of the element genome and the subsequent joining of these cleaved ends to a new target DNA site.

233 RAG initiates V(D)J recombinational joining of these gene segments to generate the large num

234 The joining of these regions with neighboring segments, wher

235 d junctions that are typically formed by the joining of three to four of the linear segments.

236 However, direct fusion joining of Ti6Al4V to SS316 can cause brittle Ti-Fe inte

237 The covalent joining of topoisomerases to DNA is normally a transient

238 RNL and Bf PNK/CPDase are sufficient for the joining of tRNA splicing intermediates in vitro, and for

239 A method is described for the joining of two alpha-lithiated C(sp(3)) stereocenters ef

240 mutants and most aberrations reflect either joining of two broken chromosomes in a "half crossover"

241 A critical overview of the catalytic joining of two different electrophiles, cross-electrophi

242 egitimate recombination characterized by the joining of two DNA ends that lack homology.

243 imic a DNA DSB, enabling us to study the end-joining of two fluorescently labeled DNA with the T4 DNA

244 s an intermolecular beta-sheet formed by the joining of two individual GFC beta-sheets and a large bu

245 RP biogenesis culminates with the joining of two large subcomplexes, the lid and base.

246 on, we used parabiotic pairing, ie, surgical joining of two mice, to create a shared circulation betw

247 s linked to an RNA enzyme that catalyzes the joining of two oligonucleotide substrates.

248 important phenomenon involving the physical joining of two plants to generate a chimeric organism.

249 orphyrin pentamer + porphyrin monomer or the joining of two porphyrin trimers.

250 otides, which catalyse the template-directed joining of two RNA molecules, one bearing a 5'-triphosph

251 h acting in end resection, also promotes end-joining of uncapped telomeres.

252 ni from DNA double-strand break ends and the joining of unmodified ends.

253 e successful deletion and non-homologous end joining of up to 725 kb reframed the DMD gene.

254 0-deficient cells were severely impaired for joining of V(D)J coding and recombination signal sequenc

255 do so, developing B cells use combinatorial joining of V-, D-, and J-gene segments to generate an ex

256 Direct joining of Vbeta segments to nonrearranged Dbeta or Jbet

257 eta to Jbeta rearrangements occurring before joining of Vbetas to a DJbeta complex.

258 Adhesive joining of veneers to cores offers potential simplicity

259 dings, we suggest that (a) nonhomologous end joining of Vlambda1 and Jlambda1 coding ends in fetal B

260 Such frequent joining of widely separated CSR DSBs could be promoted b

261 t a general DNA repair process that promotes joining of widely separated DSBs within a chromosome.

262 nerating chimeric animals through parabiotic joining of wild-type (wt) and diabetic (db/db) mice.