ライフサイエンスコーパス: karyopherin

1 ase of Ran from nuclear transport receptors (karyopherins).

2 which R-DPRs interfere with cargo loading on karyopherins.

3 to FG-repeat-containing nucleoporins through karyopherins.

4 blocks nuclear import mediated by different karyopherins.

5 he nucleus using the cellular alpha and beta karyopherins.

6 calization and interaction with its relevant karyopherins.

7 taining Nups that serve as docking sites for karyopherins.

8 nucleoporins, general transport factors, and karyopherins.

9 and dissociation of ribosomal proteins from karyopherins.

10 he adaptor nucleoporins arose from ancestral karyopherins.

11 complex, the small GTPase Ran, and cellular karyopherins.

12 the majority of binding energy for all five karyopherins.

13 ucleus by the Karyopherin beta family member Karyopherin 13 (Kap13).

14 IFN) signaling by binding to NPI-1 subfamily karyopherin alpha (KPNA) nuclear import proteins, preven

15 its interaction with the NPI-1 subfamily of karyopherin alpha (KPNA) nuclear transporters.

16 AT1 (PY-STAT1), which occurs via a subset of karyopherin alpha (KPNA) nuclear transporters.

17 of Ebola virus (EBOV) VP24 protein with host karyopherin alpha (KPNA) proteins blocks type I interfer

18 We analyzed the role of karyopherin alpha (KPNA), a key classical nuclear import

19 ng sites/regions in Saccharomyces cerevisiae karyopherin alpha (SRP1).

20 own assays indicate interaction of APE1 with karyopherin alpha 1 and 2, which requires the 20 N-termi

21 Karyopherin alpha 2 (KPNA2) is a nuclear import factor t

22 uclear import complex formation by tethering karyopherin alpha 2 and karyopherin beta 1 to the membra

23 t mutations in the C terminus no longer bind karyopherin alpha 2 or block the nuclear import of STAT1

24 We also show that N-terminal deletions of karyopherin alpha 2 that no longer bind to karyopherin b

25 mbinant SARS-CoV lacking ORF6 did not tether karyopherin alpha 2 to the ER/Golgi membrane and allowed

26 We mapped the region of ORF6, which binds karyopherin alpha 2, to the C terminus of ORF6 and show

27 ns that interact with the NLS and identified karyopherin alpha 3 (KPNA3 or Kap-alpha3) and karyopheri

28 aryopherin alpha 3 (KPNA3 or Kap-alpha3) and karyopherin alpha 4 (KPNA4 or Kap-alpha4) as key binding

29 g as an 'adapter' molecule between VirE2 and karyopherin alpha and 'piggy-backing' VirE2 into the hos

30 Interaction of karyopherin alpha and a classical nuclear localization s

31 heterodimeric receptor comprised of importin/karyopherin alpha and beta.

32 dimeric import receptor composed of importin/karyopherin alpha and beta.

33 nteraction with all six members of the human karyopherin alpha family.

34 nal significance of specific residues within karyopherin alpha for NLS cargo binding.

35 monstration that a glutathione S-transferase-karyopherin alpha fusion interacts with ORF29p, but not

36 We have also expressed each karyopherin alpha mutant in vivo as the only cellular co

37 We established that five karyopherin alpha paralogs are expressed by primary mous

38 inhibit IFN-induced gene expression or bind karyopherin alpha proteins, properties of EBOV VP24.

39 the Bag6 nuclear localization sequence from karyopherin alpha to retain Bag6 in the cytosol but also

40 A karyopherin alpha variant with a mutation in the major N

41 However, we also find that a karyopherin alpha variant with a mutation in the minor N

42 ed to the creation of conditional alleles of karyopherin alpha with well characterized defects in NLS

43 factor Srp1 (also known as importin alpha or karyopherin alpha) is required for ubiquitin-independent

44 ions as a molecular bridge between VirE2 and karyopherin alpha, allowing VirE2 to utilize the host ce

45 In addition to binding plant karyopherin alpha, VirE3 interacts with VirE2, a major b

46 p is transported into the nucleus by a Ran-, karyopherin alpha- and beta-dependent mechanism.

47 l and then imported into its nucleus via the karyopherin alpha-dependent pathway.

48 ing is also involved in the association with karyopherin alpha.

49 nt of the cellular nuclear import machinery, karyopherin alpha.

50 S cargo that is imported into the nucleus by karyopherin alpha.

51 x and is modulated through interactions with karyopherin alpha.

52 We found that yeast karyopherin alpha/Srp1p and Crm1p are required for the n

53 phorylated and imported into the nucleus via karyopherin alpha/Srp1p.

54 lves of the ring are structurally related to karyopherin-alpha (Kap-alpha) and beta-karyopherin famil

55 Nuclear import, mediated in part by karyopherin-alpha (KPNA)/importin-alpha subtypes, regula

56 e preferential interaction of W protein with karyopherin-alpha 3 and karyopherin-alpha 4.

57 on of W protein with karyopherin-alpha 3 and karyopherin-alpha 4.

58 nsportation of DP rcDNA via interaction with karyopherin-alpha and -beta.

59 ed with cellular nuclear transport receptors karyopherin-alpha and -beta.

60 hese sequences can mediate direct binding to karyopherin-alpha and are essential for the passage of i

61 cargoes and their import receptor proteins, karyopherin-alpha and karyopherin-beta, can be robustly

62 The strongest suppressor was karyopherin-alpha, a nuclear-import receptor; this requi

63 Furthermore, karyopherin-alpha, karyopherin-beta1 and the Ran GTPase

64 Overexpression of karyopherin-alpha, P32, or CidA in female flies suppress

65 ing catalytic mutant, CidB*, also identified karyopherin-alpha; the P32 protamine-histone exchange fa

66 x 10(-)(3)), and the nuclear import protein karyopherin alpha1 (KPNA [rs6810306]; P = 4.91 x 10(-)(2

67 es, that PY-STAT1 can interact not only with karyopherin alpha1 but also with karyopherins alpha5 and

68 -STAT1 interaction, indicating that the VP24-karyopherin alpha1 interaction contributes to the block

69 nteracting with the nuclear transport factor karyopherin alpha1 through its C-terminal arginine-rich

70 VP24 is found to specifically interact with karyopherin alpha1, the nuclear localization signal rece

71 Overexpression of VP24 results in a loss of karyopherin alpha1-PY-STAT1 interaction, indicating that

72 with overexpressed but also with endogenous karyopherin alpha1.

73 binding to both overexpressed and endogenous karyopherin alpha1.

74 binding region located in the C terminus of karyopherin alpha1.

75 r localization signal receptor for PY-STAT1, karyopherin alpha1.

76 ound and inhibited PY-STAT1 interaction with karyopherins alpha1, alpha5, and alpha6.

77 t VP24 inhibits interaction of PY-STAT1 with karyopherins alpha1, alpha5, or alpha6 by binding within

78 apable of binding its nuclear import adaptor karyopherin-alpha1 (KPNA1).

79 red that Nsp1beta induced the degradation of karyopherin-alpha1 (KPNA1, also called importin-alpha5),

80 y interacted with the nuclear import protein karyopherin-alpha1 but not with karyopherin-alpha2, -3,

81 ryopherin-alpha2, -3, or -4, suggesting that karyopherin-alpha1 transports nsP2 to the nucleus during

82 We found that BPV1 L2 bound to the karyopherin alpha2 (Kap alpha2) adapter and formed a com

83 VP1 and L1 capsomeres could bind both karyopherin alpha2 and DNA simultaneously.

84 n signal receptor for PY-STAT1, but not with karyopherin alpha2, alpha3, or alpha4.

85 nor a mixture of recombinant import factors (karyopherin alpha2, karyopherin beta1, Ran, and p10/NTF2

86 ation, and its interaction with the importin karyopherin alpha2.

87 port protein karyopherin-alpha1 but not with karyopherin-alpha2, -3, or -4, suggesting that karyopher

88 Capsomeres of L1, but not VP1, bound by karyopherin alpha2beta1 or beta1 alone were unable to bi

89 Both VP1 and L1 capsomeres bound by karyopherin alpha2beta1 were unable to assemble into cap

90 we found that only one of the NLS receptors, karyopherin alpha3 (Kapalpha3/Qip), would support signif

91 Mutating the nuclear importer karyopherin alpha3 also leads to reduced toxicity from p

92 ng of a Drosophila nuclear transport protein karyopherin-alpha3 (dKap-alpha3).

93 Here we report a novel correlation between karyopherin alpha4 (KPNA4) and PCa pathological stages.

94 Here, we report that KPNA4, encoding karyopherin-alpha4 (KPNA4), is exclusively amplified and

95 ) are likely to impair VP24 binding to human karyopherin alpha5 (KPNA5) and therefore inhibition of i

96 ations located in the protein interface with karyopherin alpha5 may enable VP24 to inhibit karyopheri

97 t only with karyopherin alpha1 but also with karyopherins alpha5 and alpha6, which together comprise

98 termined that importin alpha7, also known as karyopherin alpha6 (KPNA6), directly interacts with the

99 imported into the plant cell nucleus via the karyopherin alphadependent pathway and that elevated int

100 ich together comprise the NPI-1 subfamily of karyopherin alphaS.

101 ransferase pull-down assays, TDP-43 bound to karyopherin-alphas, thereby confirming the classical nuc

102 domain, we examined the relationship between karyopherin and DNA binding of both mPy VP1 and HPV11 L1

103 Knowledge of the cargoes carried by each karyopherin and insight into the mechanisms of transport

104 nucleoporin mutants as well as a few of the karyopherin and transport factor mutants also mislocaliz

105 FG domain may have the capacity to bind both karyopherins and an mRNA export factor simultaneously.

106 we show that Bcp1 dissociates Rpl23 from the karyopherins and associates with Rpl23 afterward.

107 L2 interacts via its NLSs with a network of karyopherins and can enter the nucleus via several impor

108 potent modifiers of DPR toxicity, including karyopherins and effectors of Ran-mediated nucleocytopla

109 importin-beta alone or in complex with other karyopherins and enters the nucleus via the NPC.

110 nteracts via its C-terminal NLS with several karyopherins and exploits these interactions to enter th

111 lalanine-glycine repeats (FG Nups) that bind karyopherins and facilitate the transport of karyopherin

112 s identified by mass spectrometry, most were karyopherins and nucleoporins.

113 aryopherin alpha5 may enable VP24 to inhibit karyopherins and subsequently the host interferon respon

114 ng within the PY-STAT1 binding region of the karyopherins and that this function is conserved among t

115 Thus, HPV11 L2 can interact with several karyopherins and the viral DNA and may enter the nucleus

116 s were used to investigate the role of other karyopherins, and the results suggested that rAAV2 may u

117 opose that, in the absence of Kap123p, these karyopherins are able to supplant Kap123p's role in impo

118 Preceding evidence has supported that karyopherins are associated with chemoresistance.

119 These results support a role for karyopherins as chaperones in the in vivo regulation of

120 human papillomavirus type 11 (HPV11) L2 with karyopherin beta (Kap beta) nuclear import receptors rev

121 f karyopherin alpha 2 that no longer bind to karyopherin beta 1 still retain ORF6 binding activity bu

122 rmation by tethering karyopherin alpha 2 and karyopherin beta 1 to the membrane.

123 tor Pax6 is imported into the nucleus by the Karyopherin beta family member Karyopherin 13 (Kap13).

124 e, we report that the KPNB1, a key member of Karyopherin beta subunits, is highly expressed in advanc

125 nterfering RNA screens previously identified karyopherin beta transportin-3 (TNPO3) and NPC component

126 We found that the karyopherin beta-related export receptor CRM1 competes w

127 n via a classical import mechanism utilizing karyopherin beta.

128 Transport factors in the karyopherin-beta (also called importin-beta) family medi

129 structural basis for the specificity of the karyopherin-beta family for the GTP-bound state of Ran,

130 Overexpression of karyopherin-beta Kap123, one of the ZMP-specific binders

131 ne pSTAT3 translocates to the nucleus by the karyopherin-beta nucleocytoplasmic system and binds DNA.

132 neral architecture and mechanism of the IMB3 karyopherin-beta subfamily whilst also highlighting diff

133 y soluble nuclear transport receptors of the karyopherin-beta superfamily termed importins and export

134 ighly specific inhibitor of CRM1, a cellular karyopherin-beta that transports nuclear export signal-c

135 f full-length yeast importin-beta (Kap95p or karyopherin-beta) complexed with RanGTP, which provides

136 ort receptor proteins, karyopherin-alpha and karyopherin-beta, can be robustly measured and that quan

137 madillo and Heat repeats of beta-catenin and karyopherin-beta, respectively.

138 ts show that FG domain collapse is caused by karyopherin beta1 (Kapbeta1) binding at low concentratio

139 Binding of GTP-bound Ran (RanGTP) to karyopherin beta1 (Kapbeta1) releases import cargo into

140 nuclear import of pA-RCC1 also required both karyopherin beta1 and Ran.

141 also capable of import through the importin/karyopherin beta1 pathway but was not functional in all

142 ombinant import factors (karyopherin alpha2, karyopherin beta1, Ran, and p10/NTF2) were able to suppo

143 that the transport of the classical receptor karyopherin-beta1 (Kapbeta1) is regulated so as to produ

144 clear transport and found that knockdowns of karyopherin-beta1 and cellular apoptosis susceptibility

145 Furthermore, karyopherin-alpha, karyopherin-beta1 and the Ran GTPase cycle are required

146 a1 mRNA or expression of a dominant-negative karyopherin-beta1 in a stable cell line supporting HBV r

147 nsfection of small interfering RNA targeting karyopherin-beta1 mRNA or expression of a dominant-negat

148 Karyopherin beta2 (Kapbeta2, transportin) binds the M9 s

149 Karyopherin beta2 or transportin recognizes a proline-ty

150 uman NXF1 can be imported via importin beta, karyopherin beta2, importin 4, importin 11, and importin

151 hromatography, we show that huntingtin has a karyopherin beta2-dependent proline-tyrosine (PY)-NLS in

152 oxidative stress-dependent interaction with karyopherin beta2.

153 the PY-NLS, that is recognized by the human karyopherin beta2/transportin (Kapbeta2) receptor.

154 nown pathways of protein nuclear import, the karyopherin beta2/transportin pathway is only the second

155 Karyopherin-beta2 (transportin) binds a cognate import s

156 In the nucleus, Ran x GTP binds karyopherin-beta2 and dissociates the substrate.

157 Karyopherin-beta2 contains eighteen HEAT repeats arrange

158 an GTPase with a Ran-binding domain and with karyopherin-beta2 have revealed unusually tight embraces

159 Strikingly, the nuclear import receptor Karyopherin-beta2 reverses the mutant defects and recove

160 rt receptor transportin 1 (Trn1; also called karyopherin-beta2) in an RNA-sensitive manner.

161 Here we present the 3.0 A structure of the karyopherin-beta2-Ran x GppNHp complex where GppNHp is a

162 , compared to Ran GDP, in regions contacting karyopherin-beta2.

163 ith karyopherins; each bound 6--10 different karyopherin betas, including importins as well as export

164 of the RanGTP gradient and redistribution of karyopherins between the nucleus and the cytoplasm.

165 ing that beta-catenin and importin-beta/beta-karyopherin both interact with common nuclear pore compo

166 h the ability to form ternary complexes with karyopherins, but with the capacity to potentiate RanGAP

167 Indeed, each karyopherin can interact directly with ELYS.

168 s bearing nuclear transport receptors called karyopherins can exhibit behaviour that varies from high

169 de interactions that resemble those found in karyopherin*cargo complexes and support the proposal tha

170 karyopherins and facilitate the transport of karyopherin-cargo complexes.

171 occurs in seconds and involves assembly of a karyopherin.cargo complex and docking to the NPC, transl

172 en the ectopic expression of Dalpha1 and the karyopherins CAS and importin beta1 suggest that high nu

173 s a Brownian ratchet model, in which a cargo-karyopherin complex remains bound to the same FG-Nups fo

174 ed hydrophobic residues that bind the export karyopherin CRM1.

175 pression attenuates the activity of numerous karyopherin-dependent host transcription factors (VDR, C

176 hin nuclear pore complexes (NPCs) to disrupt karyopherin-dependent nuclear-cytoplasmic transport and

177 leoporin that binds import- and export-bound karyopherins, dynamically associates with the NPC in a R

178 Nup116p exhibited generic interactions with karyopherins; each bound 6--10 different karyopherin bet

179 Transportin-SR2 (Tnpo3, TRN-SR2), a human karyopherin encoded by the TNPO3 gene, has been identifi

180 thways and readily saturated, is the nuclear karyopherin exportin-5.

181 ed to karyopherin-alpha (Kap-alpha) and beta-karyopherin family members.

182 We have identified the karyopherin family of nuclear import factors as possible

183 Crm1 is a member of the karyopherin family of nucleocytoplasmic transport recept

184 e that one of importin beta's relatives, the karyopherin family of proteins, manages this checkpoint.

185 establish importin-11 as a new member of the karyopherin family of transport receptors, and identify

186 ansport factors that are members of the beta-karyopherin family, which can bind cargo directly (e.g.,

187 oluble receptors of the importin/exportin or karyopherin family.

188 The flexible beta-karyopherin fold of Tnpo3 embraces the RNA recognition m

189 Srp1, Kap95, and Sxm1 as the most important karyopherins for Rrp6 nuclear import and the nuclear loc

190 irus type 11 (HPV11) L1 capsomeres bound the karyopherin heterodimer alpha2beta1 in vitro in a nuclea

191 The yeast karyopherin heterodimer Kap60p.Kap95p facilitates nuclea

192 TRN-SR2, Transportin-3, TNPO3) is a cellular karyopherin implicated in nuclear import of HIV-1.

193 meric import receptor consisting of the beta-karyopherin importin beta, which mediates interactions w

194 ld-type, passive export rates of a classical karyopherin/importin (Kap) Kap60p/Kap95p-targeted NLS-GF

195 calization signal (NLS) to the NLS receptor, karyopherin/importin alpha, is the most well studied nuc

196 ere we demonstrate that Kap114p, the primary karyopherin/importin responsible for the nuclear import

197 1p can bind directly to Kap114p, the primary karyopherin/importin responsible for the nuclear import

198 domain to impair docking of cargo-receptor (karyopherin/importin) complex and disrupt nuclear import

199 ross the nuclear envelope most frequently by karyopherin/importin-beta superfamily members that are c

200 of CD40 into the nucleus through the classic karyopherins (importins-alpha/beta) pathway.

201 rins) and soluble nuclear transport factors (karyopherins, importins, and exportins).

202 ulation is enhanced by addition of exogenous karyopherins/importins or RCC1, both of which also enhan

203 stones H3 and H4 is mediated by at least two karyopherins/importins, Kap123p and Kap121p.

204 , the functional relevance and regulation of karyopherins in hepatocellular carcinoma (HCC) is poorly

205 is controlled by varying the amount of free karyopherins in solution, which modulates the multivalen

206 play a role in the release of histones from karyopherins in the nucleus.

207 Karyopherins, including importin beta and its cargo adap

208 specifically competed by importin-beta/beta-karyopherin, indicating that beta-catenin and importin-b

209 an pathogenicity because they alter the VP24-karyopherin interaction, the Bombali virus amino acids m

210 portin-beta family and found that all tested karyopherins invert their subcellular distributions upon

211 The structural flexibility of beta-karyopherins is critical to mediate the interaction with

212 t nuclear import of TBP is mediated by a new karyopherin (Kap) (importin) family member, Kap114p.

213 We found that HPV16 E6 interacted with the karyopherin (Kap) alpha2 adapter and could enter the nuc

214 We have identified a novel human karyopherin (Kap) beta family member that is related to

215 ction by modulating protein interactions for karyopherin (Kap) beta family members.

216 e MSN5 was previously shown to function as a karyopherin (Kap) for nuclear export of various proteins

217 This gene product functions as a karyopherin (Kap) for nuclear import.

218 imported into the nucleus by members of the karyopherin (Kap)/importin family.

219 nd H2B is mediated by several members of the karyopherin (Kap; importin) family.

220 The yeast karyopherin Kap114p has previously been shown to import

221 f the H2A and H2B NLSs specifically with the karyopherin Kap114p.

222 , deletion of the previously uncharacterized karyopherin KAP120 caused accumulation of Rpl11b-GFP in

223 w that Nup116 mediates nuclear import of the karyopherin Kap121, and each protein is required for mit

224 s of phenotypes associated with mutations in karyopherin Kap121p.

225 t Spo12p is imported into the nucleus by the karyopherin Kap121p.

226 import cargoes for the essential yeast beta-karyopherin, Kap121p.

227 sembly factor 1 (CAF-1), as well as upon the karyopherin Kap123p, but was independent of Cac2p, anoth

228 published results our data indicate that the karyopherin Kap142p is able to mediate nuclear import of

229 is impaired in nup82-3 and in mutants of the karyopherin KAP95, but is not affected by the loss of MS

230 yeast extracts by affinity chromatography on karyopherin Kap95p-coated beads.

231 (cNLS)-mediated nuclear import supported by karyopherins (Kaps) alpha and beta1.

232 Nucleocytoplasmic transport is sustained by karyopherins (Kaps) and a Ran guanosine triphosphate (Ra

233 Karyopherins (Kaps) transport cargo across the nuclear p

234 alent interactions with transport receptors (Karyopherins (Kaps)) that orchestrate nucleocytoplasmic

235 nuclear transport receptors (NTRs), such as karyopherins (Kaps), that mediate the trafficking of nuc

236 and is mediated by soluble carriers known as karyopherins (Kaps), transportins, importins, or exporti

237 localization signals that are recognized by karyopherins (Kaps).

238 Passage across is mediated by host karyopherins (KPNAs), which bind to the viral nucleoprot

239 y regulates the subcellular distributions of karyopherins likely due to alteration of the RanGTP grad

240 isplacement of the C-terminal tail of Ran by karyopherins may be a general mechanism to facilitate Ra

241 The importin-beta family members (karyopherins) mediate the majority of nucleocytoplasmic

242 antagonizes interferon signaling by blocking karyopherin-mediated nuclear import processes.

243 d cellular state without the requirement for karyopherin-mediated nuclear import.

244 e proposed to function as stepping stones in karyopherin-mediated transport pathways.

245 ession of GSP1, the small GTPase that powers karyopherin-mediated transport, rescued mitochondrial an

246 ntify a novel link between E2 regulation and karyopherin-mediated transport.

247 f both NLS epitopes abolishes binding to the karyopherins, mislocalized NXF1 to the cytoplasm, and si

248 tic genetic interactions with mutants of the karyopherin MSN5.

249 chloric acid/aniline assay revealed that the karyopherin Mtr10 mediates retrograde import of tRNAPhe,

250 We report here that the karyopherin Mtr10p is required for the normal accumulati

251 bic residues for activity, and show that the karyopherin Nmd5p is required for Crz1p nuclear import.

252 the main transport receptor in the importin/karyopherin nuclear import pathway.

253 us are recognized by members of the importin-karyopherin nuclear transport receptor family.

254 s demonstrate a global role for Gsp1p-GTP on karyopherin-nucleoporin interactions and provide a rudim

255 s light on the importance of finely adjusted karyopherin-nucleoporin interactions for efficient cargo

256 of the nuclear transport machinery including karyopherins, nucleoporins, and the Ran guanine-nucleoti

257 CC3 binds directly to the karyopherins of the importin beta family in a RanGTP-ins

258 Soluble karyopherins of the importin-beta (impbeta) family use R

259 the nucleus by multiple transport receptors (karyopherins or importins).

260 at are recognized by import receptors termed karyopherins or importins.

261 We find that most nucleoporins and karyopherins preferentially associate with a subset of h

262 a model where cytosolic histones bind import karyopherins prior to acetylation.

263 s underlies how transport receptors known as karyopherins proceed through a tethered layer of intrins

264 in B inhibits nuclear export mediated by the karyopherin protein chromosomal region maintenance 1 (CR

265 st there are at least 14 members of the beta-karyopherin protein family that govern the movement of a

266 ransport of oncogenic factors is mediated by Karyopherin proteins during cell transformation.

267 also known as KAP114, is one of 14 importin/karyopherin proteins in yeast.

268 this analysis we have identified three other karyopherins, Pse1p/Kap121p, Sxm1p/Kap108p, and Nmd5p/Ka

269 Consistent with their ability to inhibit the karyopherin-PY-STAT1 interaction, Zaire, mouse-adapted Z

270 well as a rationale for interactions of the karyopherin-Ran complex with the regulatory proteins ran

271 f two distinct isoforms of ligand-free human karyopherin RanBP5 and investigate its global propensity

272 Since each type of karyopherin receptor prefers particular Nups or uses a l

273 biophysical, and cellular analyses show that karyopherin receptors are required to authenticate, bind

274 hree putative NLSs potentially recognized by karyopherin receptors is involved in nuclear localizatio

275 The understanding of how different karyopherins recognize human NXF1, the examination of NX

276 ved family of soluble transport factors, the karyopherins (referred to as importins and exportins).

277 exportin complex Cse1p.Gsp1p.GTP function as karyopherin release factors (KaRFs) because they can acc

278 p1p, Nup2p, Cse1p, and Gsp1p may function as karyopherin release factors (or KaRFs) in the nuclear ba

279 ctation, we show here that cells lacking the karyopherin required for Hog1 nuclear import or in which

280 It has been shown that chaperones or karyopherins responsible for import can maintain the sta

281 interactions of NXF1 homologues with various karyopherins reveals the evolutionary development of red

282 slocation of HDAC3 via the phoshorylation of karyopherin subunit alpha2 and alpha6.

283 Proteins of the karyopherin superfamily including importins and exportin

284 provide a rudimentary map of the routes that karyopherins take as they cross the nuclear pore complex

285 Kap123p is a yeast beta-karyopherin that imports ribosomal proteins into the nuc

286 netic approach in yeast, we identified three karyopherins that engage the two independent nuclear loc

287 Indeed, alpha-karyopherins that mediate nuclear import bound to alphaK

288 members of a family of transport receptors (karyopherins) that mediate the nucleocytoplasmic transpo

289 proteins (Rab6 and Vps53) in viral entry, a karyopherin (TNPO3) in viral integration, and the Mediat

290 ied proteins by mass spectrometry, and found karyopherins to be one of the major groups of proteins e

291 ing HIV-1 nuclear import, Vpr interacts with karyopherins to disturb their import of IRF3 and NF-kapp

292 tion may be a general mechanism used by beta-karyopherins to recognize transport substrates.

293 al proteins and transport them together with karyopherins to their nuclear destination.

294 ons of Nup116p and Nup100p directly bind the karyopherin transport factor Kap95p during nuclear prote

295 The karyopherin transportin SR2 (TRN-SR2, TNPO3) is responsi

296 Here, we identify beta-karyopherin Transportin-1 (TRN-1) as a cellular co-facto

297 We now find that the distantly related karyopherin, transportin, negatively regulates nuclear e

298 Two beta-karyopherins, transportin (TNPO) 1 and TNPO3, can bind C

299 iation of Ran x GTP with transport carriers (karyopherins) triggers the loading/unloading of export o

300 In addition to the karyopherins, we identified Rai1p, a protein previously

301 p153, are recognized by transport receptors (karyopherins) when trafficking large molecular cargos th