ライフサイエンスコーパス: kiss-and-run

1 cargo discharge and reducing pore closure ('kiss-and-run').

2 connection between the vesicle and surface ('kiss-and-run').

3 depolarization and (2) fusion pores (called kiss-and-run).

4 tin inhibited full-fusion more strongly than kiss-and-run.

5 sis was shifted from full-collapse fusion to kiss-and-run.

6 n pathway via alternative mechanisms such as kiss-and-run.

7 via two distinct mechanisms: full-fusion and kiss-and-run.

8 s to indicate that synaptic vesicles undergo kiss-and-run.

9 ltiple stages and control the choice between kiss-and-run and full-fusion.

10 Kiss-and-run and reuse could enable hippocampal nerve te

11 k single vesicles through multiple rounds of kiss-and-run and reuse, without perturbing vesicle cycli

12 vents associated with "full fusion" events, "kiss-and-run" and "kiss-and-stay" exocytosis, confirming

13 fusion pores, promoted fusion pore closure (kiss-and-run), and reduced late-stage fusion pore expans

14 l the choice of mode between full-fusion and kiss-and-run, and influence the dynamics of both initial

15 ivities, their preference for full fusion or kiss-and-run, and their sensitivity to inhibition by syn

16 action of fusion events has been shown to be kiss-and-run, as determined using cell-attached capacita

17 The increased incidence of kiss-and-run at lower frequencies may ensure that vesicl

18 The only genetic evidence for kiss-and-run at the synapse comes from mutations in the

19 meter, Qdots will not escape vesicles during kiss-and-run but only with full collapse fusion.

20 ible fusion process commonly referred to as 'kiss-and-run', but only rarely.

21 Alternatively, the pore may close (kiss-and-run), but the triggering mechanisms and its end

22 pocampal synapses and that the prevalence of kiss-and-run can be modulated by stimulus frequency.

23 Kiss-and-run dominated at the beginning of stimulus trai

24 The importance of kiss-and-run during efficient neurotransmission has rema

25 cape of FM 4-64, indicating that it is a non-kiss-and-run endocytic event.

26 forms of compensatory endocytosis, including kiss-and-run endocytosis and a mechanism for efficient r

27 Mechanisms, including rapid kiss-and-run endocytosis as well as local, preferential

28 tion of endophilin mutants demonstrates that kiss-and-run endocytosis is a major component of synapti

29 sed including clathrin-mediated endocytosis, kiss-and-run endocytosis, cavicapture, and bulk endocyto

30 his form of recycling is not compatible with kiss-and-run endocytosis; moreover, it is 200-fold faste

31 By extension, clathrin-independent "kiss-and-run" endocytosis does not sustain synaptic tran

32 ely 1 s by the reversal of fusion pores via 'kiss-and-run' endocytosis.

33 ion of openings that close without dilating (kiss-and-run events) enabled us to resolve exocytosis in

34 ) IV increased the frequency and duration of kiss-and-run events, but left their amplitude unchanged.

35 (KOs) increased the duration and fraction of kiss-and-run events, increased total catecholamine relea

36 y synaptic vesicle endocytosis including any kiss-and-run events.

37 and run" pathway, and that the fraction of "kiss and run" events can be increased to over 80% by sup

38 "Kiss-and-run" events and tubule connections mediate tran

39 me and revealed to precisely mark organelle "kiss-and-run" events.

40 Activity-evoked kiss and run exocytosis opens synaptic DCV fusion pores

41 radox is explained by a fourfold increase in kiss-and-run exocytosis (as determined by single-granule

42 he upregulation of STXBP6 and an increase in kiss-and-run exocytosis at the expense of full fusion.

43 synaptotagmin isoform activated, and because kiss-and-run exocytosis can filter small molecules throu

44 ely slow rate of release of glutamate during kiss-and-run exocytosis shifts the population of AMPA re

45 ion pores and survive intact for future use (kiss-and-run exocytosis).

46 f a fusing vesicle are fates associated with kiss-and-run exocytosis, and we find that these are the

47 An intact cortex favors kiss-and-run exocytosis, whereas disrupting the cortex f

48 dent partial emptying of DCVs, suggestive of kiss-and-run exocytosis.

49 incomplete exocytosis, often referred to as 'kiss and run' exocytosis.

50 ore fleeting mode of vesicle fusion, termed 'kiss-and-run' exocytosis or 'flicker-fusion', indicates

51 tsynaptic consequences, such that so-called 'kiss-and-run' exocytosis results in negligible activatio

52 In contrast, a nonclassical mode known as "kiss-and-run" features fusion by a transient fusion pore

53 ) in Dictyostelium is carried out by a giant kiss-and-run focal exocytic event during which the two m

54 uminescence change allowed us to distinguish kiss-and-run from full-collapse fusion and to track sing

55 selectively release catecholamines through a kiss-and-run fusion event.

56 l cortex plays a key role in stabilizing the kiss-and-run fusion event.

57 rimental evidence supports a predominance of kiss-and-run fusion events and rapid vesicular re-use.

58 Kiss-and-run fusion events were concentrated near the ce

59 rafast, overshoot, and bulk endocytosis, and kiss-and-run (fusion pore closure).

60 cle fusion, we found both full collapse and 'kiss-and-run' fusion at calyx-type synapses.

61 These results show that 'kiss-and-run' fusion occurs at synapses and that it can

62 ' remains controversial, and the ability of 'kiss-and-run' fusion to generate rapid synaptic currents

63 'Kiss-and-run' fusion was seen as a brief capacitance fli

64 es completely, or close rapidly to generate 'kiss-and-run' fusion.

65 Fusion pores seen during microvesicle kiss-and-run have a conductance of 19 pS, 11 times small

66 t evidence argues against the occurrence of 'kiss-and-run' in hippocampal synapses.

67 ne invagination and vesicle reformation; (b) kiss-and-run, in which the fusion pore opens and closes;

68 We find that vesicles mainly undergo kiss-and-run instead of full fusion in the absence of fu

69 Transient "kiss and run" interactions between endosomes containing

70 To study these 'kiss-and-run' interactions directly in vivo, we previous

71 Further work is needed to determine whether kiss-and-run is a major mode of fusion and has a major r

72 Kiss-and-run is a mode of membrane fusion and retrieval

73 Our data also provide further evidence that kiss-and-run is able to maintain neurotransmitter releas

74 duces mostly kissing events, suggesting that kiss-and-run is the default mode of vesicle fusion.

75 echnique to provide compelling evidence that kiss-and-run is the dominant mode of vesicle fusion at h

76 onclusions dispute previous assertions that "kiss-and-run" is a major mechanism of vesicle recycling

77 Transient fusion ("kiss-and-run") is accepted as a mode of transmitter rele

78 tion of flickering and closing fusion pores (kiss-and-run) is very well explained by the observed beh

79 as that proposed to support the presence of kiss-and-run, is likely explained by the stochastic natu

80 Kiss-and-run (KR) is an unconventional fusion between se

81 egulation between full fusion and reversible kiss-and-run-like events.

82 We propose that kiss-and-run maintains neurotransmission at active zones

83 ese data suggest the presence of a selective kiss and run mechanism of insulin release.

84 thin the readily releasable pool (RRP) via a kiss-and-run mechanism that involves rapid opening and c

85 eatedly deliver material to the vacuole by a kiss-and-run mechanism.

86 RE is associated with the transient fusion ("kiss-and-run") mechanism of transmitter release and is t

87 ypothesis that vesicle secretion involves a 'kiss-and-run' mechanism.

88 Basal sympathetic firing elicits a transient kiss-and-run mode of exocytosis and modest catecholamine

89 pore dilation and maintains the granule in a kiss-and-run mode of exocytosis.

90 th low release probability primarily use the kiss-and-run mode, whereas high release probability term

91 ween exocytosis and endocytosis, inducing a "kiss and run" mode of exocytosis and endocytosis.

92 These experiments have revealed a "kiss and run" mode of exocytosis in which synaptic vesic

93 The extent to which a "kiss-and-run" mode of endocytosis contributes to synapti

94 tch from a full fusion mode of release to a "kiss-and-run" mode of release through the transient open

95 s of vesicle retrieval: a fast (400-860 ms) 'kiss-and-run' mode that has a selective fusion pore; a s

96 Work supporting the kiss-and-run model of transient exocytosis implies that

97 Consistent with a kiss-and-run or cavicapture mode of secretion, biotinyla

98 hormones are released through a transient ('kiss and run') or an irreversibly dilating pore (full fu

99 are recovered by either fusion pore closure (kiss-and-run) or clathrin-mediated endocytosis directly

100 ucture (Omega-profile), followed by closure (kiss-and-run) or merging of the Omega-profile into the p

101 ion pore impacted the release mode to favour kiss-and-run over full-fusion.

102 about 20% of the vesicles normally use this "kiss and run" pathway, and that the fraction of "kiss an

103 they released their contents, indicating a "kiss-and-run" pathway.

104 frequent cycles of fusion and fission in a 'kiss and run' pattern.

105 cles, with long-dwelling vesicles preferring kiss-and-run rather than full-collapse fusion.

106 , including bulk retrieval and the so-called kiss-and-run recycling.

107 rom the reserve pool, placing constraints on kiss-and-run recycling.

108 ve terminals but do not preclude concurrent "kiss-and-run" recycling.

109 However, the existence of kiss-and-run remains highly controversial, as revealed b

110 es, the size of the fusion pore is unclear, 'kiss-and-run' remains controversial, and the ability of

111 ronger inhibition of full-fusion compared to kiss-and-run, somatostatin will preferentially inhibit t

112 parison with FM dye destaining revealed that kiss-and-run strongly prevailed over full-collapse fusio

113 During kiss-and-run, syt IV increased the conductance and durat

114 mbrane, where sequential compound fusion and kiss-and-run take place to enhance exo-endocytosis capac

115 e, a new mode of endocytosis termed compound kiss-and-run that enhances vesicle recycling capacity.

116 -fold higher with Syt I than Syt IV, but for kiss-and-run the Ca2+ sensitivities differed by a factor

117 During "kiss and run," the vesicle interior may be exposed very

118 st 'local cycling' near release sites (e.g. 'kiss and run' transmitter release) at low stimulus frequ

119 otein receptor (SNARE) complex that promotes kiss-and-run vesicle fusion.

120 Quickening of kiss-and-run vesicle reuse was also observed at higher f

121 tention of membrane marker, consistent with 'kiss-and-run' vesicle cycling.

122 Such 'kiss-and-run' vesicle fusion can in principle result in

123 Alternatively, in what is termed kiss-and-run, vesicles can release transmitter during tr

124 of a Ca2+ ligand in the C2A domain of Syt I; kiss-and-run was inhibited by mutation of a homologous C

125 retory vesicle collapses into the PM; or by "kiss-and-run," where the fusion pore does not dilate and

126 cycled by a second, faster mechanism called 'kiss-and-run', which operates in 1 s or less to retrieve

127 pore, the activation of isoforms that favor kiss-and-run will select smaller molecules over larger m

128 id opening and closing of a fusion pore (or "kiss-and-run") with a median opening time of 2.6 s, whic

129 regulates the choice between full fusion and kiss-and-run, with Ca2+ binding to the C2A and C2B domai

130 Nonclassical fusion retrieval by kiss-and-run would be kinetically advantageous but remai