ライフサイエンスコーパス: leader peptide

1 of tnaC, the coding region for a 24-residue leader peptide.

2 revealed three sequences that might encode a leader peptide.

3 ome during synthesis of TnaC, the 24-residue leader peptide.

4 this sequence is only part of a much longer leader peptide.

5 d of the normally expressed 7 amino acid pro leader peptide.

6 on a dominant secretion signal in the alpha-leader peptide.

7 vels of tryptophan and synthesis of the TnaC leader peptide.

8 motif, and HMGL51 contained a mitochondrial leader peptide.

9 he mitochondrion using a yeast mitochondrial leader peptide.

10 was mediated by an N-terminal sec-dependent leader peptide.

11 amphiphilic behavior is due to its uncleaved leader peptide.

12 region is typical of that of a mitochondrial leader peptide.

13 2 signal sequence substituting for the HLA-E leader peptide.

14 file is characteristic of the amino-terminal leader peptide.

15 protease fused to a decahistidine-containing leader peptide.

16 ly reversible and has no specificity for the leader peptide.

17 sequence bypassed the need for the pre-Cox2p leader peptide.

18 ded proteins: indeed mammalian Cox2p lacks a leader peptide.

19 n by cleavage between Arg-3 and Val-2 in the leader peptide.

20 sible for the removal of the entire propilin leader peptide.

21 ion analysis of ftpA confirmed the lack of a leader peptide.

22 ported from their cytoplasm by an N-terminal leader peptide.

23 , the coding region for the operon-specified leader peptide.

24 ne residues of propilin are found within the leader peptide.

25 n specifically rather than a property of the leader peptide.

26 hat TraQ recognition may be specific for the leader peptide.

27 sulted in an alkyne at the C-terminus of the leader peptide.

28 he protein to act on core peptides without a leader peptide.

29 protein with a hydrophobic 25-aa N-terminal leader peptide.

30 d 5'-end heterogeneity and encoded alternate leader peptides.

31 otein sequencing established the presence of leader peptides.

32 licus XylF has typical features of bacterial leader peptides.

33 e distinct from that for the canonical short leader peptides.

34 y the lack of a general method to remove the leader peptides.

35 consisting of PSA with a serine-arginine pro leader peptide ([-2]pPSA) instead of the normally expres

36 containing the construct without introns or leader peptide accumulated undetectable amounts of PAT1-

37 the presence of excess tryptophan, the TnaC leader peptide acts in cis on the ribosome translating t

38 dies suggest that during induction, the TnaC leader peptide acts in cis on the translating ribosome t

39 results support the hypothesis that the TnaC leader peptide acts in cis to alter the behavior of the

40 These data suggest that the N-AgrD leader peptide affects S. aureus biology in a manner sim

41 However, the leader peptide also presents drawbacks as it needs to be

42 Here we demonstrate that the leader peptides alter the conformation of domains IV and

43 ylococcus aureus requires both an N-terminal leader peptide and a C-terminal cell wall sorting signal

44 -grasp ligase CdnC bound to ADP, a conserved leader peptide and a peptide substrate.

45 (Phe) led to continued synthesis of the PheL leader peptide and attenuation of pheA transcription und

46 solated following proteolytic removal of the leader peptide and can incorporate a wide range of amino

47 e gain-of-function mutants of a Tat-specific leader peptide and for the genetic analysis of the "inva

48 The site-directed mutagenesis of bMSTN gene leader peptide and in vitro expression in bovine fibrobl

49 Western blot analysis showed that Shr has a leader peptide and is found in both membrane-bound and s

50 reading frame (uORF) specifying a 24-residue leader peptide and is subject to a novel form of negativ

51 ha chain differed from wild type only in its leader peptide and mature NH2-terminal amino acid.

52 Finally, we explored the roles of the leader peptide and of the RiPP precursor peptide recogni

53 r cargo proteins following processing of the leader peptide and prior to ATP-dependent alternating ac

54 g either the leader peptide (cox2-20) or the leader peptide and processing site (cox2-21) without alt

55 ocated close to the boundary of the putative leader peptide and secreted protein.

56 ed protein contained a typical mitochondrial leader peptide and showed 92 and 54% homology to RMIP an

57 from this compartment, since deletion of the leader peptide and the cytoplasmic location of MucB abro

58 The unusual propilin leader peptide and the dependence of its cleavage on Tra

59 A second fusion gene encoded the TraA leader peptide and the first 51 amino acids of F-pilin i

60 tutively activated using covalently attached leader peptides and a GNAT-type N-acetyltransferase.

61 fusions between a set of eight putative Tat leader peptides and alkaline phosphatase in isogenic Esc

62 to Brassica napus cytosolic CyP, contain no leader peptides and include a conserved seven amino-acid

63 omigrates with the full-length 51-amino-acid leader peptide, and [14C]proline labeling confirmed its

64 Additionally, CcaM does not require the leader peptide, and this core-dependence effectively set

65 es demonstrate the presence of an N-terminal leader peptide, and three other domains in the following

66 oding genes, regulatory RNA genes and operon leader peptides, and we analyzed the transcriptional app

67 nly the eight C-terminal amino acids of this leader peptide are required for maturation of MccJ25.

68 ence of thioether bridges and removal of the leader peptide are required for RumC's antimicrobial act

69 Leader peptides are relatively unusual in mitochondriall

70 It is not known why leader peptides are removed by the mitochondrial process

71 ine core from two dehydroalanines ejects the leader peptide as a C-terminal carboxamide.

72 allow us to propose a putative role for the leader peptide as a simple motif for docking of the McjA

73 ATG start codons, and a unique mitochondrial leader peptide as well as 15 additional amino acids of c

74 der peptidase enzyme, demonstrating that the leader peptide at the N-terminus or PilA or XcpT did not

75 In the spatial positioning model, leader peptide binding promotes certain reactions by est

76 high content of lysine residues in the McjA leader peptide, but herein we also demonstrate that thes

77 that two CtAs are bound via their N-terminal leader peptides, but only one is positioned for cleavage

78 rminal fragment and coupling to a 38-residue leader peptide by means of native chemical ligation (NCL

79 ) of a PCAT subsequent to the removal of the leader peptide by the PEP domain.

80 ecognition of the secondary structure of the leader peptide by the synthetase.

81 st step of the pathway is the removal of the leader peptide by ThoK to afford mature thioholgamide.

82 Only the HLA alleles that possess a leader peptide capable of upregulating HLA-E surface exp

83 A typical 29-aa leader peptide, characteristic of secretory proteins in

84 onsecutive post-translational modifications: leader peptide cleavage, followed by methylation of the

85 provide evidence that translation of the tet leader peptide coding region blocks tet expression by pr

86 nslational regulation by rtpLP, a 10 residue leader peptide coding region located immediately precedi

87 he 115-bp spacer region separating tnaC, the leader peptide coding region, from tnaA.

88 nase operon requires that translation of its leader peptide coding region, tnaC, be coupled with its

89 equires in-frame translation of a 24-residue leader peptide coding region, tnaC, that contains a sing

90 ction is dependent on translation of a short leader peptide coding region, tnaC, that contains a sing

91 ding to ribosomes that translate the tetA(L) leader peptide coding sequence.

92 ene results in ribosome stalling in the ermC leader peptide coding sequence.

93 ting translation of tandem Trp codons in the leader-peptide coding region of the operon.

94 and regulatory functions of the at operon's leader peptide-coding region, rtpLP, of B. subtilis and

95 duction requires translation of a 24-residue leader peptide-coding region, tnaC, containing a single,

96 ing of the three Trp codons in its at operon leader peptide-coding region.

97 Geobacillus YqeH fused to a putative AtNOA1 leader peptide complements growth and morphological defe

98 Third, highly positively charged leader peptide conjugates including HIV-Tat-NaKtide (pNa

99 dimorphism in exon 1 of HLA-B gives rise to leader peptides containing methionine (Met; M) or threon

100 We propose that the pre-Cox2p leader peptide contains a targeting signal necessary for

101 ibosomes engaged in the synthesis of TnaC, a leader peptide controlling the expression of the Escheri

102 s, stalling of ribosomes on the 3-amino-acid leader peptide could favor formation of the Hp1-Hp2 stru

103 S. cerevisiae COX2 gene, removing either the leader peptide (cox2-20) or the leader peptide and proce

104 chia coli by using a gene construct with the leader peptide deleted and fused to a 38-kDa E. coli pro

105 se domain in complex with a covalently bound leader peptide demonstrates the basis for substrate reco

106 that generation of the thioether linkage is leader-peptide-dependent, suggesting that this reaction

107 in an ordered, but nonlinear fashion that is leader-peptide-dependent.

108 he murine class Ib molecule Qa-1b presents a leader peptide derived from D-end class I molecules to a

109 its human homolog, HLA-E, predominantly bind leader peptides derived from other class I molecules.

110 osition -21 in the gene segment encoding the leader peptide dictates whether NK cell regulation prima

111 The pre-Cox2p leader peptide did not signal translocation.

112 n based on the combination of cis-regulatory leader-peptide elements with genetically encoded unnatur

113 It is shown here that slowing of the leader peptide elongation rate, either by cycloheximide

114 our additional lipoproteins with OspE/F-like leader peptides (Elps) and demonstrated that all are enc

115 up of lipoproteins which possess OspE/F-like leader peptides (Elps).

116 gion suggests that this polypeptide may be a leader peptide encoded by a transcriptional attenuator.

117 strict sequence specificity is necessary for leader peptide engagement.

118 attached to the ATP-grasp ligases to examine leader peptide/enzyme interactions.

119 construct utilizing the naturally occurring leader peptide express recombinant protein outside of th

120 r structure consisting of a highly conserved leader peptide followed by an N-terminal proline-rich do

121 peptide chain is composed of a 21-amino-acid leader peptide, followed by 578 amino acids of the matur

122 es and the dependence of an eight-amino acid leader peptide for activity.

123 ynthetic fasciculin gene with an appropriate leader peptide for expression and secretion from mammali

124 nt forms of Qa-1b that are dependent on this leader peptide for its maturation, nor did it inhibit th

125 ent that sequences of interest be fused to a leader peptide for modification.

126 We show that fusing the leader peptide for the lantibiotic lacticin 481 to its b

127 The bacterial pheL gene encodes the leader peptide for the phenylalanine biosynthetic operon

128 emonstrate that this protease can remove the leader peptide from a diverse set of peptides.

129 The 51-amino-acid TraA leader peptide fused directly to alkaline phosphatase wa

130 xported from the cytoplasm by means of a Tat leader peptide fused to the heavy chain alone, indicatin

131 chimeric protein with the propilin wild-type leader peptide fused to the mature portion of beta-lacta

132 ICFP, encoding the yeast prepro-alpha factor leader peptide fused via a lysine-arginine cleavage site

133 simulations support a mechanism in which the leader peptide guides posttranslational modification by

134 th full-length newly synthesized Cox2, whose leader peptide had already been cleaved in the IMS.

135 at the enzyme is functional when most of the leader peptide has been removed.

136 Additionally, leader peptides have undergone extensive N-terminal degr

137 rotease fused to a deca-histidine containing leader peptide (His-protease) have been cloned, expresse

138 tion in their ability to cleave the prepilin leader peptide; however, two double mutants did exhibit

139 The role of the leader peptide in lantibiotic biosynthesis has been subj

140 tational modeling to examine the role of the leader peptide in the biosynthesis of a homolog of strep

141 ptide recognition, we observe binding of the leader peptide in the catalytic barrel rather than the N

142 By using nascent leader peptides in combination with bound specific amino

143 e show serves a purpose similar to canonical leader peptides in directing the biosynthetic enzymes to

144 We also found that translation of a leader peptide increases rplJL expression, presumably by

145 Moreover, we demonstrate the first leader peptide-independent activity of a TOMM synthetase

146 tion of OlvS(A) activity showed that it is a leader peptide-independent and S-adenosyl methionine-dep

147 C-to-N terminal directional process that is leader-peptide-independent.

148 The Staphylococcus aureus ErmCL leader peptide induces stalling in the presence of clini

149 ated that tryptophan induction might involve leader peptide inhibition of ribosome release at the sto

150 When the structure of the leader peptide is altered via mutations resulting in mor

151 For bioengineering purposes, the leader peptide is beneficial because it allows promiscuo

152 dies revealed that the Thr-35 residue in the leader peptide is critical for correct processing of Mcj

153 other RiPPs such as cyanobactins wherein the leader peptide is not engaged at all.

154 The 15-amino acid leader peptide is processed after export to the intermem

155 he mitochondrial matrix as a precursor whose leader peptide is rapidly processed by the inner membran

156 rified protein revealed that a 19 amino acid leader peptide is removed from DotA prior to secretion.

157 form and provides an explanation for why the leader peptide is removed from the precursors.

158 The rRNA target for binding the leader peptides is therefore conserved across kingdoms.

159 g of trimethylamine N-oxide reductase (TorA) leader peptide libraries resulted in isolation of six ga

160 at lacked the globular domain and the signal leader peptide (LP) signal sequence (amino acids 1 to 22

161 In this study, we identified the 18-kDa leader peptide (LP18) of the foamy virus envelope protei

162 rocyclization and cleavage of the N-terminal leader peptide, making them potentially useful biocataly

163 been predicted by encoded transmembrane and leader peptide motifs.

164 des to the ribosome prompts insertion of the leader peptide MsrDL into a crevice of the ribosomal exi

165 The results indicated that leader peptide mutation resulted in down-regulation of P

166 l RiPP leaders called nitrile hydratase-like leader peptides (NHLPs) are longer and structured.

167 ibosome stalling during translation of ErmBL leader peptide occurs in the presence of the antibiotic

168 of a protease involved in the removal of the leader peptide of a class III lanthipeptide.

169 A 24-amino acid leader peptide of a new human recombinant manganese supe

170 When the entire hydrophobic domain of the leader peptide of FR-beta (12 amino acid residues) was s

171 Herein we have investigated the role of the leader peptide of McjA and found that only the eight C-t

172 e of the first 11 amino acids of the gene 10 leader peptide of phage T7 (T7.Tag) and the putative sig

173 on to generate a C-terminal thioester of the leader peptide of ProcA, the substrate of ProcM.

174 t is shown that the interactions of p25, the leader peptide of subunit IV of cytochrome c oxidase, wi

175 RNA(Pro), the peptidyl-tRNA precursor of the leader peptide of this operon.

176 and minor levels of [-4]pPSA, containing pro leader peptides of 2 and 4 amino acids, respectively.

177 r is selected by a cis effect of the nascent leader peptide on its translating ribosome.

178 duction might involve cis action by the TnaC leader peptide on the ribosome translating the tnaC codi

179 synthetic events, such as proteolysis of the leader peptide or transport of the active compound outsi

180 Stalling of ribosome at the PA5471 leader peptide (PA5471.1) mRNA leads to transcription of

181 rometry analysis, we show here that the LctA leader peptide plays critical roles in establishing the

182 nt in the endoplasmic reticulum (ER) to trim leader peptide precursors into the appropriate epitope o

183 are synthesized as precursors that include a leader peptide, presumed to direct the polypeptide to th

184 de bridge, and (v) high-level expression and leader peptide processing are achievable in Escherichia

185 Pre-Cox2 leader peptide processing by Imp1 occurs in the absence

186 , the Cox20 chaperone has important roles in leader peptide processing, C-tail export, and stabilizat

187 class Ib molecule Qa-1(b) binds the class Ia leader peptide, Qdm, which reacts with CD94/NKG2R on NK

188 n rules dictate catalysis, and we posit that leader peptide recognition imposes substrate selectivity

189 rovide a model for understanding the role of leader peptide recognition in the translocation cycle, a

190 hus, unlike conventional cytosolic peptides, leader peptides (regardless of whether they are dependen

191 ing aspartyl protease involved in release of leader peptide remnants from the endoplasmic reticulum m

192 Herein we investigate the leader peptide requirements for TclM from thiocillin bio

193 ive N-terminal degradation and the remaining leader peptide residues have been N-succinylated.

194 orporation of helix-breaking residues in the leader peptide resulted in greatly decreased proteolytic

195 Like the leader peptides SecM and TnaC, the FXXYXIWPP peptide ind

196 us, despite its unusual length, the propilin leader peptide seems to perform a function similar to th

197 of an extra adenine in the last codon of the leader peptide sequence (TAC-->TAAC) leading to a nonsen

198 f peptide libraries derived from a nonameric leader peptide sequence randomized at individual anchor

199 r- and oxygen-dependent and do not require a leader peptide sequence.

200 d binding of peptides derived from the known leader peptide sequences of other rat histocompatibility

201 Qa1b-specific peptides Qdm and HLA-CW4 leader peptide specifically inhibited the lysis of these

202 wever, introduction of Pro residues into the leader peptide strongly affected the efficiency of dehyd

203 of binding regardless of the presence of the leader peptide, suggesting that although the PEP domain

204 which contained an N-terminal 25-amino-acid leader peptide terminated by a signal peptidase I cleava

205 dition of proline, rather than inhibition of leader peptide termination.

206 peptide processing site, suggesting a larger leader peptide than expected.

207 s 2-15 and encodes a different mitochondrial leader peptide than Variant I.

208 se proteins is the presence of a specialized leader peptide that is cleaved off by a cognate membrane

209 nvolves a precursor peptide which contains a leader peptide that is important for the modification pr

210 recursor peptide also contains an N-terminal leader peptide that is required to guide the biosyntheti

211 IgG transgenes were controlled by a leader peptide that targets the transgene products to th

212 sulting in a change of Leu to Pro within the leader peptide that would be predicted to inhibit secret

213 The cargo proteins have N-terminal leader peptides that are cleaved off by the cysteine pro

214 ible for endopeptidic cleavage of the unique leader peptides that characterize type-IV pilin precurso

215 Leader peptides that induce stalling can regulate downst

216 eveals a pathway for antigen presentation of leader peptides that involves translocation of a protein

217 that the McpM precursor peptide includes two leader peptides that undergo sequential cleavage at posi

218 Introns interrupt the sequences encoding the leader peptide, the FAD-binding site, the calcium-bindin

219 eam of the structural gene does not encode a leader peptide, the information to target the protein fo

220 Sequences encoding the leader peptide, the proline-rich region, and the repeat

221 Following removal of the leader peptides, the same enzyme is responsible for the

222 Although these proteins lack a leader peptide, their presence in the extracellular medi

223 Regulation by this eukaryotic leader peptide thus appears to be exerted through a nove

224 Synthesis of the leader peptide, TnaC, is essential for antitermination.

225 osomes stall on the secretion monitor (SecM) leader peptide to regulate expression of a downstream ge

226 his alkyne in turn was used to conjugate the leader peptides to a variety of synthetic peptides by co

227 le as intercellular mediators, most lack the leader peptide typically required for protein secretion.

228 binding domain can engage sequence-divergent leader peptides using a conserved motif that facilitates

229 n E. coli which results in connection of the leader peptide via an ester linkage that is readily clea

230 The proteolytic removal of the leader peptide was also performed in vitro.

231 A 37-amino acid leader peptide was cleaved sequentially by two mitochond

232 The leader peptide was hypothesized to inhibit ribosome rele

233 mination demonstrated that processing of the leader peptides was highly accurate.

234 se lipoproteins all contain highly conserved leader peptides, we now propose that the ancestors of ea

235 l other conserved amino acid residues in the leader peptide were targeted by site-directed mutagenesi

236 s (underlined) in the newly synthesized TnaC leader peptide, WFNIDXXL/IXXXXP.

237 he open reading frame encode a 34-amino-acid leader peptide whereas the mature protein is composed of

238 ydrophobic domains of the GPI signal and the leader peptide with the endoplasmic reticulum.

239 ids, including the 26 amino acid amphipathic leader peptide with the Gly(-2)-Gly(-1) sequence at the

240 Synthetic leader peptides with recognition sequences for up to thr