ライフサイエンスコーパス: ligation reaction

1 ion or phosphodiester synthesis steps of the ligation reaction.

2 nd 3'-ends of the RNA into proximity for the ligation reaction.

3 opies of itself through an RNA-catalyzed RNA ligation reaction.

4 ereo-specificity of the heme-apocytochrome c ligation reaction.

5 Lys251 and Asp253 at different steps of the ligation reaction.

6 random sequences, performs an efficient RNA ligation reaction.

7 ne functional groups to the hairpin ribozyme ligation reaction.

8 t concentrations as high as 50 microM in the ligation reaction.

9 ing RNAs because the cP inhibits the adapter ligation reaction.

10 required for the strand closure step of the ligation reaction.

11 ing, at the end distal to the join, slow the ligation reaction.

12 -mRNA splicing before the first cleavage and ligation reaction.

13 d the ligase during pre- and post-step 3 the ligation reaction.

14 ing the pre- and post-catalytic steps of the ligation reaction.

15 oken strand breaks in the three steps of the ligation reaction.

16 an be further derivatized in a bioorthogonal ligation reaction.

17 enabled enzyme library screening for peptide ligation reaction.

18 circularized in a strictly target dependent ligation reaction.

19 mes in which decarboxylation is coupled to a ligation reaction.

20 emical evidence has been established for the ligation reaction.

21 oach that enabled the reconstitution of this ligation reaction.

22 -5'-AMP), the obligatory intermediate of the ligation reaction.

23 ucidation of the mechanism of WaaL-catalyzed ligation reaction.

24 ynthesized peptide using the native chemical ligation reaction.

25 and catalytic mechanism of the kinetoplastid ligation reaction.

26 riction-enzyme-digested vectors prior to the ligation reaction.

27 sion through the three chemical steps of the ligation reaction.

28 ined by Watson-Crick base pairing during the ligation reaction.

29 inhibition of the hairpin-catalyzed RNA-RNA ligation reaction.

30 f miRNA-target chimeras formed by endogenous ligation reactions.

31 ctive groups on protein sequences for use in ligation reactions.

32 ure have been examined in the BrCN activated ligation reactions.

33 xhibited quite different effects on the same ligation reactions.

34 omains is required for both the cleavage and ligation reactions.

35 ions has classically focused on bond-forming ligation reactions.

36 of an a-helix to control strand cleavage and ligation reactions.

37 arcodes are appended to the cDNA via in-cell ligation reactions.

38 late groups from DNA that arise from aborted ligation reactions.

39 e labeling using in vitro selective chemical ligation reactions.

40 e regioselectivity of ribozyme-catalyzed RNA ligation reactions.

41 icks or breaks that result from abortive DNA ligation reactions.

42 ithout the need for restriction digestion or ligation reactions.

43 yme catalyzes site-specific RNA cleavage and ligation reactions.

44 DNA sites and catalysis of DNA cleavage and ligation reactions.

45 e for sequence-specific primer extension and ligation reactions.

46 In the ligation reaction, a 2'-5' RNA phosphodiester linkage is

47 plants that can perform site-specific, rapid ligation reactions after a target peptide asparagine/asp

48 Unexpectedly, ligation reactions also occurred in the absence of the e

49 f this reaction limits the efficiency of the ligation reaction and has become a significant constrain

50 t compared to their effects on the composite ligation reaction and individual upstream steps.

51 uorescence protein (GFP) through an in vitro ligation reaction and the 17.8-kb-long X-inactive-specif

52 ibuting to the remarkable specificity of the ligation reaction and the physiological reaction conditi

53 increasing the rate constant of the surface ligation reaction and thus the probability of immobiliza

54 al domain that executes the DNA cleavage and ligation reactions and a smaller amino-terminal domain t

55 aphylococcus aureus sortase A for a range of ligation reactions and demonstrate that conditions can r

56 uential cleavage, U addition or removal, and ligation reactions and is directed by complementary guid

57 luded the standard enzyme digestion-mediated ligation reactions and the subsequent isolation of plasm

58 DNA fragments in ligation reactions are capable of combining to produce n

59 The tyrosine ligation reactions are shown to be compatible with the l

60 tivated 4-mer PNAs that react in a templated ligation reaction at muM concentrations within minutes.

61 n described a hundred years ago in 1919, the ligation reaction became one of the most important and e

62 ere extended by further amino acid residues, ligation reactions became slower.

63 addition to oligosaccharide sequencing, the ligation reaction between an oligosaccharide and an amin

64 s model is based on the L-21 Sca I catalyzed ligation reaction between exogenously added oligomers: c

65 Monitoring probe hybridization and ligation reactions by electrophoretic mobility retardati

66 limitations of the canonical native peptide ligation reaction catalyzed by sortase A.

67 porated into the cell wall through a peptide ligation reaction catalyzed by transpeptidase sortase.

68 repair pathway, namely the DNA synthesis and ligation reactions catalyzed by E. coli DNA polymerase a

69 This self-selecting ligation reaction could be restarted by only a few major

70 We have found that the rate of the trans ligation reaction depends on pH, corresponding to the pr

71 ible; the pyrophosphate leaving group in the ligation reaction does not induce 2',5'-cleavage, and py

72 Here, the connection between ligation reaction efficiency and the retention of enzyme

73 de an unprecedented insight into the role of ligation reaction efficiency in mediating the exploratio

74 We then show that loop-closing ligation reactions enable the assembly of full-length fu

75 ults revealed that IntDOT is able to perform ligation reactions even when all the bases within the cr

76 e C-C cross-link does not interfere with the ligation reaction, even when it is located only two base

77 that incorporates the SNP specificity of the ligation reaction for more effective clinical management

78 ternal" equilibrium between the cleavage and ligation reactions for the circular hammerheads was shif

79 ntacts and conformational changes propel the ligation reaction forward.

80 The native chemical ligation reaction has been used extensively for the synt

81 A template-free click-ligation reaction has been used for the intramolecular c

82 This ligation reaction has similarities to the reaction catal

83 It is based on the use of a specific gap ligation reaction, horseradish peroxidase (HRP) for sign

84 DNA ligases catalyze a NAD(+)-dependent DNA ligation reaction, i.e., the formation of a phosphodiest

85 pendent conversion of two rapid photoinduced ligation reactions, i.e., the light activation of o-meth

86 d to a larger total surface area for the RNA ligation reaction; (ii) the SiNPs enhance the diffusion

87 s that theoretically may both participate in ligation reactions, implying that potentially not only p

88 L), combines the PCR and the oligonucleotide ligation reaction in a two-stage thermal cycling sequenc

89 ormations that may help us to understand the ligation reaction in FPGS and influence the design of me

90 is catalyzed by the ribozyme catalyzing the ligation reaction in its deprotonated state (rate 1.05 m

91 intermediate during the initial step of the ligation reaction in the presence of an A:C mismatch at

92 orming a truncated helix 7 that promotes the ligation reaction in vitro.

93 technique relies upon a simple, preliminary ligation reaction in which target DNA sequences are conv

94 Upon transformation of the ligation reaction into Escherichia coli, infectious phag

95 alf of the selected deoxyribozymes mediate a ligation reaction involving the natural branch-point ade

96 7, the equilibrium constant (K(eq)) for the ligation reaction is 3.89 x 10(4) m.

97 The Mn2+-dependent branch-forming ligation reaction is between an internal branch-site 2'-

98 hioalkyl esters are rather unreactive so the ligation reaction is catalyzed by in situ transthioester

99 The ligation reaction is effectively irreversible; the pyrop

100 The ligation reaction is monitored by Forster resonance ener

101 A self-catalyzed mechanism for this cleavage-ligation reaction is presented, based on mutagenesis dat

102 The requirement of 3' complementarity for a ligation reaction is reaffirmed by results from 1 nt ins

103 The SrtA-mediated ligation reaction is reversible, so most labeling protoc

104 as the conserved peptide, whose role in the ligation reaction is unknown.

105 a rapid, copper-free, tetrazine-cyclopropene ligation reaction (k2 > 5 M(-1) s(-1)).

106 ntly attached to the surface by an enzymatic ligation reaction (leaving the anti-sense strand dissoci

107 In this method, DNA hybridization and ligation reactions led to the attachment of ATRP initiat

108 m and the tight coupling of the cleavage and ligation reactions make it difficult to characterize the

109 of uncatalyzed RNA backbone cleavage, their ligation reactions may be of direct relevance to the RNA

110 catalytic determinants for the cleavage and ligation reactions mediated by the hairpin ribozyme are

111 Treatment of the ligation reaction mixture with exonuclease prior to ampl

112 3' A overhang by including 0.5 M NaCl in the ligation reaction mixture.

113 of peptide or protein products directly from ligation reaction mixtures by Ni-NTA affinity column pur

114 The native chemical ligation reaction (NCL) involves reacting a C-terminal p

115 and enhancements have been conducted in the ligation reaction, notably by embedding an extra thymine

116 nous nucleobase rescue for both cleavage and ligation reactions now allow us to refine models of the

117 functionalized sialic acid derivative with a ligation reaction of a fluorogenic tetrazine, allowing f

118 +)-dependent deoxyribozymes that mediate the ligation reaction of an RNA 5'-hydroxyl group with a 2',

119 The DNA cleavage-ligation reaction of DNA topoisomerase I was investigate

120 A bis-ligation reaction of S-nitrosothiols using triaryl subst

121 The ligation reaction of tetrazole functionalized surfaces w

122 ificant roles in modulating the DNA cleavage/ligation reaction of the enzyme and its response to anti

123 Optimum pH for the ligation reaction of the human telomere sequence ranges

124 familiarize the reader with the DNA cleavage/ligation reaction of topoisomerase II and other aspects

125 The DNA ligation reaction of topoisomerase II is essential for g

126 The DNA cleavage/ligation reaction of topoisomerase II is the target for

127 e found that nonenzymatic, template-directed ligation reactions of oligoribonucleotides display high

128 were investigated by comparing cleavage and ligation reactions of ribozyme variants with A38 modific

129 However, simple RNA self-cleavage and ligation reactions offer a unique opportunity to measure

130 However, optimizing such multi-parametric ligation reactions often involves extensive trial and er

131 In principle, performing the ligation reactions on a solid support would eliminate th

132 ing (SPRI) measurements of surface enzymatic ligation reactions on DNA microarrays is demonstrated.

133 method has been used to analyze the multiple ligation reactions onto radiolabeled DDAH 4PteGlu 1 cata

134 hairpin ribozyme-mediated self-cleavage and ligation reactions participate in processing RNA replica

135 s from a random sequence pool in a templated ligation reaction reduced the sequence space of product

136 lization and a general base may catalyze the ligation reaction required for prebiotic RNA assembly.

137 hundred peptides were obtained in a one-pot ligation reaction, selected by affinity against MDM2 imm

138 Following the ligation reaction, SpyTag is cleaved off, rendering PBSL

139 uring the adenylyl transfer and nick-sealing ligation reaction steps.

140 Analysis of directionality of the ligations reactions suggests that for each of the Thermu

141 Cl(2)/H(2)O medium proved to be best for the ligation reactions, suppressing the undesired azide redu

142 activity was found to be required for an end-ligation reaction that circularizes a portion of the uni

143 zation, an efficient one-step chemical sugar ligation reaction that does not require prior sugar prot

144 Here we introduce a chemoselective ligation reaction that harnesses the reactivity of Witti

145 ation of exogenous lipoate is catalyzed by a ligation reaction that proceeds via a lipoyl-adenylate i

146 to highly specific self-cleavage and protein ligation reactions that are useful protein engineering t

147 ck" is often applied inaccurately to polymer ligation reactions that fail to respect the criteria tha

148 After an introduction to the bioorthogonal ligation reactions that have been used in in connection

149 res are two sequential pairs of DNA cleavage/ligation reactions that proceed via a 3' phosphotyrosine

150 moval of adenylates that arise from abortive ligation reactions that take place at incised abasic sit

151 During ligation reactions, the donor fluorophore europium (Eu(3

152 Thus, we can directly monitor the ligation reaction through the transition from dynamic to

153 g ligated products in template-promoted self-ligation reactions, thus yielding multiple signals per t

154 adily by camptothecin than the corresponding ligation reaction to form a fully complementary duplex;

155 the acceptor strand was not required for the ligation reaction to proceed, but duplex formation to pr

156 sor oligonucleotides expand the scope of the ligation reaction to reagent-free, mild conditions.

157 ites coupled with simultaneous digestion and ligation reactions to create just one product, by conver

158 hout this tool, the powerful native chemical ligation reaction typically used to assemble polypeptide

159 Despite the use of these thiol catalysts, ligation reactions typically take 24-48 h.

160 aryotic genomes is ensured by three-step DNA ligation reactions used by ATP-dependent DNA ligases.

161 ated ssDNA is ready to be circularized via a ligation reaction using a bridging oligonucleotide.

162 the bisphosphate and carried out successive ligation reactions using T4 RNA ligase and T4 DNA ligase

163 The relative observed rate of the ligation reaction was a minimum of 35 times faster on th

164 The ligation reaction was carried out using an N-terminal re

165 hat the activity of human Cdc34 in the Ub-Ub ligation reaction was enhanced dramatically by SCF's cor

166 omerase I-mediated mismatch formation in the ligation reaction was inhibited more readily by camptoth

167 The ligation reaction was rapid for a broad range of substra

168 nt base ionization events in the VS ribozyme ligation reaction, we performed nucleotide analogue inte

169 bstitution, the facility of the cleavage and ligation reactions were altered.

170 nkages accompanying all 16 stepwise cyanomet ligation reactions were experimentally resolved, only tw

171 Both ligation reactions were performed in aqueous buffered so

172 s for ATP and DNA substrates, in the overall ligation reaction, were 0.4 microM and 30 nM, respective

173 elity (HiFi) DNA ligases at each step of the ligation reaction, which has parallels to the ribonucleo

174 The nonenzymatic ligation reactions, which are characteristic of base pai

175 This efficient DNA ligation reaction will facilitate development of robust

176 ninfectious subgenomic replicons in a single ligation reaction with >80% efficiency.

177 ctrodes apparently because of a slow nitrite ligation reaction with Rh(III); however, a significant i

178 The flow EPL platform enables efficient ligation reactions with high recoveries of target protei

179 lycosylated residue were shown to facilitate ligation reactions with peptide thioesters, and these pr

180 Comparison of oligonucleotide ligation reactions with previously characterized single

181 the polymer film and their strength of axial ligation reactions, with a selectivity pattern of 1-buty

182 Arg-54 and Lys-119 abolished the overall RNA ligation reaction without affecting steps 1 and 3.

183 This mild aqueous tyrosine ligation reaction works over a broad pH range and expand

184 uential chemical synthesis and enzymatic DNA ligation reactions yield an encoded library in which ind