ライフサイエンスコーパス: limiting dilution

1 erformed with clonal populations obtained by limiting dilution.

2 -derived clonal populations were obtained by limiting dilution.

3 fusion protein, and the cells were cloned by limiting dilution.

4 urrent methods that rely on washing steps or limiting dilution.

5 ity than WT-infected LCLs for regrowth after limiting dilution.

6 T-cell clones were obtained by limiting dilution.

7 itute secondary lethally irradiated hosts in limiting dilution.

8 ultures, and multiple clones were derived by limiting dilution.

9 s, eight CD4+ T-cell clones were obtained by limiting dilution.

10 l origins by cloning using multiple steps of limiting dilution.

11 and cell lines were recovered by cloning at limiting dilution.

12 feeder layers for cloning lymphoid cells by limiting dilution.

13 d shorter tumor-free intervals in vivo after limiting dilution.

14 cells and clonal isolates were generated by limiting dilution.

15 established 18 single MSC-derived clones by limiting dilution.

16 17(+) CD4(+) T-cell clones were generated by limiting dilution.

17 lity, and generated tumour grafts in vivo at limiting dilutions.

18 TCC were generated from PBMC by limiting dilution against a mixture of eleven 20-mer ove

19 Limiting dilution analyses (LDA) detected no EBV-specifi

20 By limiting dilution analyses and index sorts, we found tha

21 s of responder cell frequency (RCF) based on limiting dilution analyses are laborious, and alternativ

22 Limiting dilution analyses for IL-2-producing cells and

23 Limiting dilution analyses indicated that an oncogenic K

24 Cell mixing and limiting dilution analyses provide evidence that these c

25 Flow cytometric and limiting dilution analyses suggest that the increase in

26 In this study we describe FACS-based limiting dilution analyses using the dilution of the int

27 Using limiting dilution analyses, the frequency of HSV-specifi

28 In limiting dilution analyses, we found that the Abbot assa

29 ity in transwell assays and clonogenicity in limiting dilution analyses.

30 cell-mediated lympholysis assays (CML), and limiting dilution analyses.

31 r relapse, and LSC frequency was assessed by limiting dilution analyses.

32 as indicated by Ag-induced proliferation and limiting dilution analyses.

33 panded in the presence of IL-2 and tested in limiting dilution analysis (LDA) for reactivity to synth

34 Limiting dilution analysis (LDA) of infiltrating cells r

35 Limiting dilution analysis (LDA) of SIC to determine pre

36 Limiting dilution analysis (LDA) results were also nonli

37 cine endothelial and lymphoblastoid cells by limiting dilution analysis and analyzed the responding T

38 re PR1-specific CTLs than were identified by limiting dilution analysis and correlated better with ly

39 esponses to all 3 viruses were quantified by limiting dilution analysis and staining with HLA-A*0201

40 te with similarly high cloning efficiency in limiting dilution analysis as CD28(+) CD45RO(hi) cells o

41 Furthermore, in vivo limiting dilution analysis assays revealed that LDN19318

42 ontrols; because of low cell numbers, formal limiting dilution analysis could only be performed in 10

43 Limiting dilution analysis demonstrated a 10-fold increa

44 Limiting dilution analysis demonstrated that proliferati

45 36 mo after transplantation were studied in limiting dilution analysis for allopeptide reactivity.

46 chromium release assay for CTL activity and limiting dilution analysis for CTL precursor frequency,

47 Limiting dilution analysis from these experiments showed

48 Limiting dilution analysis identified an expanded popula

49 Limiting dilution analysis identified one predominant CT

50 Limiting dilution analysis indicated that the frequency

51 Limiting dilution analysis indicated that the T cell pre

52 L responses against different virus strains, limiting dilution analysis is particularly appropriate t

53 ecific CD8+ T cells has been accomplished by limiting dilution analysis of cytotoxic precursor cells.

54 Similarly, as determined by limiting dilution analysis of IL-2-producing cells, T ce

55 Limiting dilution analysis of residual alloantigen-react

56 Limiting dilution analysis of TLR activated, latently in

57 Limiting dilution analysis of unfractionated blood demon

58 Sequential limiting dilution analysis of virus-specific CD4+ T cell

59 nd multiply spliced viral RNAs combined with limiting dilution analysis provided measurements of tran

60 Similarly, limiting dilution analysis revealed a greater frequency

61 Limiting dilution analysis revealed a high precursor fre

62 Limiting dilution analysis showed a 5- to 6-fold higher

63 Results of limiting dilution analysis suggested that the increase i

64 Limiting dilution analysis suggests that the kidney marr

65 Limiting dilution analysis suggests that these cells can

66 e used monocyte-derived dendritic cells in a limiting dilution analysis to generate Mtb-specific CD8(

67 ral tissues have not been evaluated, we used limiting dilution analysis to measure the concentration

68 d distinguish patients at risk, we performed limiting dilution analysis to quantify anti-EBV cytotoxi

69 equency of BCR-ABL(+) cells as determined by limiting dilution analysis was consistently higher in 38

70 CD1-, and nonclassically restricted cells, a limiting dilution analysis was performed.

71 Limiting dilution analysis was used to assess helper T-l

72 Limiting dilution analysis was used to characterize nume

73 Limiting dilution analysis was used to quantify the loca

74 ells in peripheral blood were quantitated by limiting dilution analysis, and their effect on EBV load

75 Using limiting dilution analysis, CD8(+) T cells isolated duri

76 equencies of 1 in 10(5)-10(6) as measured by limiting dilution analysis, estimates of the T cell freq

77 Using long-term BM cultures and limiting dilution analysis, G0CD34+ cells were found to

78 than, CTL precursor frequencies derived from limiting dilution analysis, indicating that this novel p

79 requencies of helper and cytotoxic cells, by limiting dilution analysis, serially measured in vitro c

80 of keratinocytes into subcutis combined with limiting dilution analysis, to assess the long-term repo

81 Here, by limiting dilution analysis, we anatomically map the deve

82 Using limiting dilution analysis, we demonstrate that the abso

83 Using a limiting dilution analysis, we found the frequency of SR

84 ated cell sorting, cell transplantation, and limiting dilution analysis, we were able to identify the

85 ong term immunologic memory as determined by limiting dilution analysis, with memory T cells displayi

86 Limiting dilution analysis-derived minimal frequency est

87 All recipients had limiting dilution analysis-detectable, graft-reactive cy

88 herwise underestimated with the conventional limiting dilution analysis.

89 e of techniques exploiting the principles of limiting dilution analysis.

90 incorporation of [3H]thymidine into DNA and limiting dilution analysis.

91 xic (CTLf) T cells were enumerated by use of limiting dilution analysis.

92 because few cells score as LCMV specific in limiting dilution analysis.

93 ), and cytotoxic T cell precursors (pCTL) by limiting dilution analysis.

94 s of the clade B of HIV-1 were determined by limiting dilution analysis.

95 lopeptide reactive T cells was determined by limiting dilution analysis.

96 -lymphocyte precursor (CTLp) frequency using limiting dilution analysis.

97 the HIC and MLR populations, as assessed by limiting dilution analysis.

98 We assessed tumorigenicity using limiting dilution analysis.

99 cellular interferon-gamma (IFN-gamma) and by limiting-dilution analysis (LDA) of cytotoxic T-cell pre

100 Limiting-dilution analysis indicates that Hem-End coloni

101 Limiting-dilution analysis of lymphocytes from animals t

102 M+, IgG+, and RF+ B cells were determined by limiting-dilution analysis of purified peripheral blood

103 l HCMV-specific CTL response, we have used a limiting-dilution analysis system to quantify HCMV-speci

104 antigen-specific memory B cells by means of limiting-dilution analysis, and we compared memory B-cel

105 Using bulk culture analysis and limiting-dilution analysis, we have shown that a replica

106 The DNA material was quantified using limiting-dilution analysis; the mean concentration was d

107 s demonstrated by colony-forming capacity in limiting dilution and by transplantation in Matrigel plu

108 to that of MC progenitors (MCp) measured by limiting dilution and clonal expansion with maturation.

109 ccuracy and sensitivity of this platform for limiting dilution and digital PCR applications.

110 of Rare EpiAlleles by Melt), which uses semi-limiting dilution and precise melt curve analysis to dis

111 lations at a single cell level by cloning at limiting dilution and propagating the resulting CTL clon

112 verified its role as a gastric CSC marker by limiting dilution and serial transplantation assays.

113 Limiting dilution and single-cell experiments show that

114 We demonstrate by limiting dilution and single-cell transplantations that

115 sed seronegative persons were then cloned at limiting dilution and tested for HCV-specific CTL activi

116 levels of cell-surface CD95 were isolated by limiting dilution, and analyzed for sensitivity to CD95-

117 f shorter tumor-free intervals in vivo after limiting dilution, and enhanced migration in invasion as

118 e cells were then restimulated and cloned by limiting dilution, and the clones were analyzed.

119 Using a limiting dilution approach, we found that many individua

120 ed from unseparated bone marrow (BM) through limiting dilution approaches.

121 transplant patients were investigated using limiting dilution assay (LDA) and ELISPOT for interferon

122 cytokine staining, and precursor frequency (limiting dilution assay [LDA]) cytotoxicity assays.

123 lloreactive cytotoxic T lymphocyte clones by limiting dilution assay at 120 days.

124 D34(+) label-retaining cells, and an in vivo limiting dilution assay for keratinocyte stem cells with

125 er transplant.For subject 2, a pretransplant limiting dilution assay revealed T helper cells recogniz

126 Finally, we developed an in vivo limiting dilution assay to compare individual T cell pro

127 We developed a tat/rev induced limiting dilution assay to measure the frequency of CD4(

128 cant decline in tumor formation frequency by limiting dilution assay upon subcutaneous administration

129 Limiting dilution assay was used to calculate the freque

130 bility of HTLV-2 to transform lymphocytes, a limiting dilution assay was used to generate clonal, tra

131 s is particularly relevant in the setting of limiting dilution assay where cells are transplanted in

132 f repopulating cells, as assessed by extreme limiting dilution assay.

133 nfected beta TCR KO mice, as demonstrated by limiting dilution assay.

134 R-incompatible individuals in bulk MLC or in limiting dilution assay.

135 did not initiate or propagate leukemia in a limiting dilution assay.

136 , as demonstrated by both cytotoxicity and a limiting-dilution assay for the estimation of CTL precur

137 particles was assayed for active vector by a limiting-dilution assay to evaluate transduction efficie

138 mphocyte (CTL) and helper T lymphocyte (HTL) limiting dilution assays (LDA) were performed 10 days af

139 Using a variety of techniques, including limiting dilution assays (LDA), intracellular IFNgamma a

140 ng FAK inhibition was evaluated with extreme limiting dilution assays as well as with immunoblotting

141 hypothesis of clonal deletion vs anergy, CTL limiting dilution assays cultures were restimulated with

142 methylcellulose colony-forming units and by limiting dilution assays in vitro.

143 In limiting dilution assays of CD34+Lin- cells, TPO was fou

144 In vivo limiting dilution assays reveal hundreds more precursor

145 Limiting dilution assays revealed that AID expression wa

146 Limiting dilution assays showed a high frequency of anti

147 CD34+ cells cultured in limiting dilution assays showed a sixfold decrease in NK

148 CTL limiting dilution assays showed that CTL precursor frequ

149 Limiting dilution assays suggest the likely mechanism of

150 The Cw3 transfectants were used in limiting dilution assays to estimate the CTL precursor f

151 sented which permits an extended analysis of limiting dilution assays under conditions in which eithe

152 olated peripheral blood mononuclear cells in limiting dilution assays were used to measure HIV-1 Gag-

153 es representing these forms were isolated by limiting dilution assays, and each replicated in cell cu

154 cell (LT-HSC) frequency, as demonstrated by limiting dilution assays, and the expanded HSPCs were ca

155 In limiting dilution assays, approximately 25% of unselecte

156 lts in a 10-fold expansion of HSCs in serial limiting dilution assays, indicating that early lymphoid

157 In limiting dilution assays, subcutaneous tumor formation w

158 As determined by limiting dilution assays, the pCTL frequency (p/f) per C

159 + T cells, as detected by IL-2 production in limiting dilution assays, to each of two class II peptid

160 in the number of BCCs, as measured by colony-limiting dilution assays, was observed.

161 Using limiting dilution assays, we examined the CD4+ T cell pr

162 T lymphocytes than traditional proliferative limiting dilution assays.

163 d clonogenicity of LTC-ICs was determined by limiting dilution assays.

164 ed reduction of tumor-initiating capacity in limiting dilution assays.

165 es have been examined only by using in vitro limiting-dilution assays (LDAs).

166 aneous autoreactive T cells, as estimated by limiting-dilution assays.

167 resistance selection as well as a regime of limiting dilution at early stages of drug treatment to p

168 ling and computer simulation were applied to limiting-dilution, autologous-transplantation studies in

169 mal cells, sorted cells were transplanted at limiting dilution before and after being cultured for 2

170 ntiviral delivery of MELK-specific shRNA and limiting dilution cell transplantations, we showed that

171 Limiting dilution clones were generated from three bulk

172 Limiting dilution cloning analysis revealed that a major

173 Limiting dilution cloning and analysis of coexpressed TC

174 We used limiting dilution cloning and synthetic peptide Ags to c

175 for 72 h with 5-aza-dC and then subjected to limiting dilution cloning.

176 tb39-specific T cell clones were obtained by limiting dilution cloning.

177 by treatment with 5-aza-2'-deoxycytidine and limiting dilution cloning.

178 rvet subspecies (Chlorocebus pygerythrus) by limiting-dilution coculture, quantitative PCR for viral

179 ing HSCs present in murine bone marrow after limiting dilution competitive transplantation.

180 We used a limiting-dilution competitive repopulation assay with ce

181 one marrow (BM) cells were injected, using a limiting-dilution, competitive transplantation method, i

182 The results of previous limiting-dilution, competitive-repopulation studies in 4

183 , and then examined for tumorigenicity under limiting dilution conditions and clonogenic activity in

184 Under limiting dilution conditions, patients' cells also showe

185 ere cultured on stromal cells and IL-7 under limiting dilution conditions.

186 Limiting dilution CTL assays determined that B6 splenocy

187 The results of a limiting dilution CTL frequency assay were not informati

188 hing T cell clones derived from the joint by limiting dilution culture confirmed coexpression of high

189 ells before and after augmented treatment by limiting-dilution culture of resting CD4+ T cells after

190 omplex class I-derived synthetic peptides in limiting dilution cultures and then compared with contro

191 Limiting dilution data suggest that at least 1 in 65 000

192 Here, we characterized 300 limiting dilution-derived virus isolates from the plasma

193 At limiting dilutions, donor T cell reconstitution of the s

194 otoxic activity as measured in both bulk and limiting dilution effector frequency assays.

195 ain tissue of ovine PrP mice inoculated with limiting dilutions (endpoint titration) of ovine AS isol

196 pervariable region 1 (HVR1), using end-point limiting-dilution (EPLD) technique, from 127 cases invol

197 Limiting dilution ex vivo cultures of resting CD4(+) T c

198 urgdorferi infection and healthy controls in limiting dilution experiments and assessed proliferation

199 Limiting dilution experiments using MPeMSCs and endothel

200 here and holoclone assays as well as in vivo limiting dilution experiments, in 13 patient-derived sam

201 Limiting-dilution experiments demonstrated that the freq

202 Limiting-dilution experiments demonstrated that the limi

203 and HIV-1 IIIB were serially measured using limiting dilution followed by in vitro stimulation with

204 erformed by laser-capture microdissection or limiting dilution, followed by careful exponential cDNA

205 HIV-1-specific CTL lines were derived by limiting dilution following Ag-specific stimulation of P

206 Isolation of T cell clones by limiting dilution from tumor-infiltrating lymphocytes (T

207 we generated 346 short term T cell lines at limiting dilutions from six normal individuals to tetanu

208 ic T cells will proliferate extensively at a limiting dilution in response to infections.

209 A limiting dilution in vivo transplantation assay was used

210 CTL frequency was measured by limiting dilutions in five patients.

211 easure of infectious HIV-1 titers involves a limiting dilution-infection assay and a calculation of t

212 3 patients, who received T cells produced by limiting dilution methods, but persisted 5 to 9 weeks in

213 t HSC expansion in culture, as determined by limiting dilution mouse reconstitution analyses.

214 hese separate populations were quantified by limiting-dilution nested polymerase chain reaction.

215 Digital PCR (dPCR) exploits limiting dilution of a template into an array of PCR rea

216 lones isolated from 4 vaccinated patients by limiting dilution of bulk tumor-reactive T cells solely

217 fusivities approximately 10(-3) m(2) s(-1)), limiting dilution of FIB and other runoff-associated pol

218 The pattern of cytokines produced from limiting dilution of naive T cells demonstrated that the

219 ) offers absolute quantification through the limiting dilution of template nucleic acid molecules and

220 antitative viral outgrowth assays (QVOA) use limiting dilutions of CD4+ T cells to measure the size o

221 m xenograft tumors efficiently in vivo using limiting dilutions of cells.

222 Limiting dilutions of CMV-seropositive or -seronegative

223 d from seminiferous tubules, were mixed with limiting dilutions of dispersed mammary epithelial cells

224 s (including the NK precursors), seeded with limiting dilutions of the cells to be quantitated, cultu

225 ecific T cells in the expanded population by limiting dilution or by using fluorescently labeled tetr

226 rowell array platform, combining sampling by limiting dilution or FACS, with imaging and high through

227 munized with spores and were cloned twice by limiting dilution or growth on semisolid medium.

228 ized RBCs or parasite cloning is achieved by limiting dilution or micromanipulation.

229 Limiting-dilution orthotopic xenografts of these BCSCs r

230 es were compared to sequences recovered from limiting-dilution outgrowth assays of resting CD4(+) T c

231 f cells reactivating gammaHV68 in vitro) and limiting dilution PCR (frequency of cells carrying gamma

232 HIV was detected and quantified by limiting dilution PCR and genetically characterized in t

233 Limiting dilution PCR has become an increasingly useful

234 We also describe a discriminatory limiting dilution PCR method that can be used to optimiz

235 mice carry latent gammaHV68, we developed a limiting-dilution PCR assay to quantitate the frequency

236 liminary study of 7 lung recipients, we used limiting-dilution PCR to quantify peripheral blood micro

237 qualitative-, quantitative-, real-time- and limiting dilution-PCR methods, are discussed.

238 heavy chain variable region patient-specific limiting-dilution polymerase chain reaction (PCR).

239 Limiting dilution precursor frequency assays demonstrate

240 r than that for B6 anti-BALB/c determined by limiting dilution proliferation assays.

241 n techniques (NAATs) in digital format using limiting dilution provides potential advantages that hav

242 n- and mixed-phase hybridization formats and limiting dilution quantitative PCR.

243 fied resting memory cell subpopulations by a limiting-dilution, quantitative viral outgrowth assay (Q

244 We used limiting dilution reactivation (frequency of cells react

245 Limiting dilution reconstitution of lethally irradiated

246 bent spot-forming cell (ELISPOT) assay and a limiting dilution responder cell frequency (RCF) assay.

247 population of B. burgdorferi, and cloning by limiting dilution resulted in the identification of two

248 mployed array-based transcript profiling and limiting-dilution reverse transcription-PCR (RT-PCR) met

249 Limiting-dilution reverse transcription-PCR confirmed th

250 Limiting-dilution reverse-transcribed PCRs confirmed dow

251 enus-specific 16S rRNA was quantified with a limiting dilution RT-PCR assay.

252 iplex reaction to detect the HIV-1 genome in limiting dilution samples followed by sequence verificat

253 po(-/-) recipients or controls, we performed limiting-dilution secondary transplants using donor cell

254 transduced with a lacZ retrovirus, cloned by limiting dilution, seeded onto a physiologic dermal subs

255 present in <3% of genomes were confirmed by limiting dilution sequencing.

256 nalysis of CTL precursor (pCTL) frequency by limiting dilution showed that the frequency of pCTL to t

257 Results from limiting dilution studies and cell-mixing experiments su

258 Limiting dilution studies indicated that mutations prese

259 In vivo, limiting dilution studies provided evidence that Rad51ap

260 d that DN thymocytes could transmit AML, and limiting dilution studies showed that leukemia initiatin

261 Limiting dilution studies with cell cultures derived fro

262 Limiting-dilution subisolates (LDSIs) of each strain wer

263 nes, and cloned tumor cells were obtained by limiting dilution techniques.

264 not require external calibration, end-point limiting dilution technologies, collectively termed "dig

265 At limiting dilutions, the d-AQuA reactions follow a stocha

266 By limiting dilution, there is a tight correlation between

267 in patients with or without CP by end-point-limiting dilution titer.

268 blood from each flow-through was measured by limiting dilution titration.

269 ions enriched from coelomocytes were used in limiting dilutions to obtain samples of single cells tha

270 ation, and antimicrobial agents, followed by limiting dilution transplant to germ-free mice and micro

271 imaging and their rarity when measured with limiting dilution transplant.

272 Limiting dilution transplantation and competitive repopu

273 human breast cancer, we have identified, by limiting dilution transplantation and in vitro mammosphe

274 Using a limiting dilution transplantation assay in immunocomprom

275 Two recent studies use fate-mapping and limiting dilution transplantation assays to identify SRY

276 Limiting dilution transplantation assays were performed

277 Leukemia onset was delayed, and limiting dilution transplantation experiments demonstrat

278 y stem/progenitor cell activity as judged by limiting dilution transplantation experiments of primary

279 Using limiting dilution transplantation performed on p53 null

280 Finally, limiting dilution transplantation revealed that p53 null

281 Single cell limiting dilution transplantation with bone marrow from

282 In limiting dilution transplantation, they proliferated to

283 Using in situ genetic lineage tracing and limiting dilution transplantation, we have unravelled th

284 R-1 in PAR-1-deficient cells combined with a limiting-dilution transplantation assay demonstrated the

285 However, limiting-dilution transplantation showed a decrease in t

286 Results from limiting dilution transplantations indicate higher frequ

287 Limiting dilution transplants further demonstrated that

288 n, as assayed by immunophenotypic markers or limiting dilution transplants, we observed increases in

289 s exhibit little to no stem cell activity in limiting dilution transplants.

290 We performed limiting dilution, transwell, and cell viability assays

291 oid-specific B cells by culturing B cells in limiting dilution upon irradiated CD40L-expressing EL4.B

292 n in a standard culture assay, as well as by limiting dilution using multiple short-term T-cell lines

293 Limiting dilution was shown to be essential for correct

294 Ten clones obtained by limiting dilution were all CD4+ and of a T-helper-1-like

295 roximately 180 viral genomes were present in limiting dilutions where as few as 16 cells were examine

296 E/nestin:EGFP-sorted cells were multipotent: limiting dilution with clonal expansion as neurospheres,

297 timulation of T cells from IL-7R -/- mice at limiting dilution with immobilized Abs to CD3, CD4 or CD

298 Progenitors were cultured in limiting dilutions with interleukin-7 (IL-7), flt3 ligan

299 precursor cells were defined by competitive limiting dilution without enrichment, tissue culture, or

300 by CD1d1-reactive T cell clones isolated by limiting dilution without preselection for NK1 expressio