ライフサイエンスコーパス: linearize

1 face, the averaged pressure values could be linearized.

2 rO) bonds and the trans U-O-C(ipso) angle is linearized (157.67 degrees versus 147.85 degrees and 130

3 A (MA) but not mitomycin C (MC) cross-linked linearized (32)P-pBR322 DNA in the presence of dithiothr

4 teractions are highly nonlinear, they can be linearized accurately with a unique method, termed Dual-

5 and architecture in spiking networks rely on linearizing activity around a central operating point an

6 The linearized Ad DNAs for active viral replication polyplex

7 A fully linearized analog, in which all six cysteines were repla

8 ate nanopores provide an effective method to linearize and thread full-length proteins in a single fi

9 Smc2/4 binds both linearized and circular plasmids, and the binding appear

10 The current may be linearized and extrapolated to further reduce the measur

11 This vector is then linearized and introduced into a recombination-proficien

12 This modification results in attachment of a linearized and monoglycosylated derivative of enterobact

13 Simulated step responses of the linearized and nonlinear models are close and in good ag

14 model library, the cyclic depsipeptides are linearized and released from the solid support to the li

15 inserting an ermAM cassette, and then it was linearized and transformed into S. gordonii DL1 for alle

16 Spectroscopic evidence for such linearized and twisted structures was found in the pigme

17 Overall, this paper, for the first time, linearizes and reduces the order of the experimentally v

18 y diseases were 99.0%, 97.1%, and 84.2% with linearized annual recurrent regurgitation of 0.63%, 2.19

19 lymer dynamics inherently nonlinear, and the linearizing approximations required for the normal-mode

20 tion, indicating the recognition of a longer linearized arabinan epitope.

21 ics with rotational degrees of freedom, once linearized around a non-equilibrium steady state charact

22 In this study, the linearized arterial stress-strain relationship is implem

23 A is terminated when the transferred strand, linearized at the 38 base-pair origin of transfer (oriT)

24 Each of these was linearized at unique sites in the vectors and YAC arms w

25 ed utilizing the all electron full-potential linearized augmented plane wave (FLAPW) approach across

26 lculations performed with the full-potential linearized augmented plane wave (FLAPW) method show that

27 i-substituted compounds using full potential linearized augmented plane wave (FPLAPW) method under ge

28 lbox is relatively low as the preparation of linearized baculoviral DNA only requires standard reagen

29 me), a nonlinear model had to be used, and a linearized biexponential (LinBiExp) model proved very ad

30 oblem of shock-wave interaction based on the linearized boundary conditions of MHD.

31 e chromosome of A. actinomycetemcomitans was linearized by digestion with a restriction endonuclease

32 The vector is then linearized by digestion with nicking and restriction end

33 on, and rapidly assemble into hydrogels when linearized by disease-associated enzymes.

34 ith either one or two restriction sites were linearized by EcoR124I at similar rates, although the tw

35 53mer uracil-containing scaffold to M13mp7L2 linearized by EcoRI.

36 sented on a 21-in. computer display suitably linearized by gamma correction.

37 This plasmid is linearized by in vivo expression of the meganuclease I-S

38 or in the absence of substrates but could be linearized by preincubating the enzyme with inhibitor, a

39 ids but does bind to plasmids that have been linearized by treatment with ionizing radiation.

40 ithmic encoding mechanisms, which are later "linearized" by education.

41 Phosphodiesterases (PDE-As) end signaling by linearizing c-di-GMP to 5'-phosphoguanylyl-(3',5')-guano

42 Linearized, centromere-containing plasmid DNA with the 5

43 of the serial assembly was first achieved by linearizing circular plasmid dsDNA using BamH I endonucl

44 or mismatch site, the nicked templates were linearized close to either site and assayed.

45 cells, bound to type I collagen (Col I), and linearized Col I via its cysteine-rich C-terminal (CT) d

46 Loss of Smurf2 also led to more linearized collagen bundles in normal and wounded skin.

47 o cleave an internal phosphodiester bond and linearize concatemers during rolling circle replication.

48 Recently, a novel linearized constitutive model with a new strain measure

49 AR cloning vector was constructed that, when linearized, contained a small amount (381 bp) of 3' hypo

50 This phosphodiesterase linearizes cyclic bis(3'-->5')diguanylic acid, an allost

51 blished by lipofection and microinjection of linearized cytomegalovirus (CMV)-CTCF construct into fer

52 production (Delta VE/Delta VCO(2)), and the linearized Delta tidal volume/Delta VE (Delta VT/Delta l

53 sible for attachment of a C-glycosylated and linearized derivative of enterobactin, an iron scavenger

54 dition strongly inhibited the effects of its linearized, disulfide-free variant, [A6, A8, A13, A15]pr

55 Linearized DNA and plasmids containing Histoplasma telom

56 Transcription of genes introduced as linearized DNA can be serially assayed for weeks in each

57 gene copies were made based on circular and linearized DNA conformations using two genomes from each

58 Thus, the end of a linearized DNA fragment can initiate new DNA synthesis b

59 r, we had found that in wild-type (WT) cells linearized DNA is degraded to completion by RecBCD nucle

60 Optical mapping of linearized DNA molecules is a promising new technology f

61 rejoined molecules revealed that >95% of the linearized DNA were precisely end-joined.

62 recise absolute quantification of RNA, short linearized DNA, or genomic DNA.

63 In the absence of ions, cyclophilins bind linearized DNA.

64 at cells compared to PC12 cells in rejoining linearized DNA.

65 the repair template is a single-stranded or linearized double-stranded DNA.

66 ) DNA and can separate single-strand nicked, linearized (double-strand nicked), fully relaxed, partia

67 CeP3W cleaves linearized, duplex DNA as well as supercoiled plasmid.

68 ed this approach to generate accelerated and linearized dynamics, sinusoidal oscillations with desire

69 of systems that are governed by a linear (or linearized) dynamics.

70 V. cholerae, a bacterium that utilizes linearized Ent, can also be weakly targeted.

71 d in detail with full mathematical rigor for linearized equatorial primitive equations with source te

72 tide (Ku70) to enhance cellular uptake and a linearized expression vector to induce enhanced expressi

73 Fragments are then combined with linearized expression vectors, assembled in vitro as par

74 -Edwards distance (CSE) performs better than linearized F(ST) The correlation (R) between the model's

75 l (1D) continuous wavelet transform (CWT) of linearized fluorescence resonance energy transfer (FRET)

76 the resulting bicyclic peptide can be easily linearized for MS/MS sequencing with a one-step deallyla

77 Minute virus of mice (MVM) replicates via a linearized form of rolling-circle replication in which t

78 ed using known concentrations of D-Lac and a linearized form of the equilibrium expression.

79 larized DNA molecule than from the identical linearized form.

80 , they have been studied extensively using a linearized formalism.

81 l for specific proteins, domain details in a linearized format, and post-translational modification c

82 t models for the ion distribution, exact and linearized forms of the planar Poisson-Boltzmann equatio

83 ), maximally-localized-Wannier-function, and linearized gap equation with random-phase-approximation,

84 ating in vacuum is derived directly from the linearized general relativistic wave equation alone, for

85 Linearized generalized estimating equation analyses rela

86 tion, since in vitro production of mRNA from linearized GFP plasmid was also blocked by caging and su

87 The log TOF-eta relationship can often be linearized, giving rise to a Tafel law, which allows the

88 had macular cRORA at baseline, with a median linearized growth rate of 0.23 mm/y (IQR, 0.22-0.25 mm/y

89 The median linearized growth rate of atrophic lesions was 0.23 mm/y

90 Numerical simulation with the full linearized Hamiltonian shows that significant amount of

91 force microscopy-based analysis that uses a linearized-Hertz method to measure individual mechanical

92 We further map our linearized, homogenized system to a non-Hermitian, nonre

93 We have artificially introduced linearized HPV18 genomic DNA into primary keratinocytes

94 rectification in symmetrical solutions, are linearized in nonsymmetrical solutions that bring the K+

95 h in which their path integral expression is linearized in the difference between forward and backwar

96 0 CTG.CAG repeats or 43 CGG.CCG repeats were linearized in vitro near the center of the repeats and w

97 ry studies, as amide bond hydrolysis forms a linearized isobaric sequence with the addition of a wate

98 able image coregistration model based on the linearized iterative boundary reconstruction (LIBR) appr

99 A linearized Langmuir adsorption isotherm was used to obta

100 ternative conformations, branched (BMH) and 'linearized' (LDI) and switch between them to achieve dif

101 Significantly, we show that linearized macrocycles can be sequenced with lower recal

102 Eigendecomposition of the linearized mean-field contact network model reveals func

103 ghted nonlinear regression (WNLR) and a fast linearizing method known as Patlak analysis.

104 lternative to Patlak analysis and other fast linearizing methods for parametric imaging.

105 To test this hypothesis, we injected a linearized, methylated, and partially single-stranded re

106 The PCR method specifically amplified whole linearized minicircle kinetoplast DNA (kDNA) of the Leis

107 Here, we extend a simple linearized model [Christensen et al., Astrophys.

108 lysis of the characteristic equation for the linearized model demonstrates the existence of a Hopf bi

109 A linearized model for small strains is also obtained as v

110 the variable selection method in the locally linearized model in CONTIN, were examined using the five

111 A linearized model is also re-analysed to calculate the ho

112 The analytically derived control-oriented linearized model is of third order and relates small dev

113 s well as smaller proteins, and feed them as linearized molecules into a protease chamber.

114 The linearized mortality rate was 3.1%/patient-year (porcine

115 inds competitively to a segment of mRNA of a linearized mRNP, passing through the NPC on its way to t

116 y higher relaxivities than the corresponding linearized multimers (Gd(2)C2: r(1) = 10.5 mM(-1) s(-1)

117 In addition, widespread presence of several linearized nontarget TPs was confirmed for the first tim

118 ted to evaluate the influence of selectively linearizing one or the other supercoiled domains created

119 We analyze a lossy linearized optomechanical system in the red-detuned regi

120 n of performance for these stellarators than linearized or local theories that suffer from a failure

121 e determined for fast nonlinear diffusion by linearizing Otto's gradient descent model at the Barenbl

122 Here we show the efficient linking of linearized PACs containing alpha satellite DNA from chro

123 cins 6 and 13 efficiently cross-linked EcoRI-linearized pBR322 DNA upon addition of Et3P.

124 ross-linking was detected in the reaction of linearized pBR322 DNA with 1,12-diaziridinyl-4,9-diazado

125 e continuous presence of ZD 7288, reversibly linearized peak V-I and instantaneous I-V relationships

126 ectra reveals the N-terminal amino acid on a linearized peptide sequence definitively and, thus, sign

127 calculations combined with exact solution of linearized phonon Boltzmann equation.

128 l that integrates self-attention mechanisms, linearized phrase structure, and a contextualized transf

129 OHC somatic electromotility is modeled by linearized piezoelectric relations whereas the OHC hair-

130 LacI with a high affinity (Osym) operator on linearized plasmid (2500 bp) DNA over a wide range of ma

131 recombination events were observed when the linearized plasmid carried short terminal regions homolo

132 mpared it with expression from a transfected linearized plasmid containing an integrated provirus.

133 nal control fragment recovered from the 125I linearized plasmid did not exhibit endo IV sensitivity i

134 of linear DNA in Escherichia coli cells, we linearized plasmid DNA at a specific site in vivo and mo

135 odel end joining of restriction endonuclease linearized plasmid DNA in whole cell extracts.

136 The simultaneous delivery of siRNA and linearized plasmid DNA on the surface of a single nanocr

137 xamples, we used different formulations of a linearized plasmid DNA solution considered for character

138 One requires NHEJ of linearized plasmid DNA transformed into the test organis

139 system leverages asymmetric end-labeling of linearized plasmid DNA using a simple "Klenow fill-in" r

140 ar recombinogenic recircularization (IRR) of linearized plasmid DNA was used to study mechanistic rel

141 Complexes formed in vitro using linearized plasmid DNA were imaged and measured by atomi

142 Dmc1 renatures linearized plasmid DNA with first order reaction kinetic

143 DNA end-joining was measured by incubating linearized plasmid DNA with mitochondrial protein extrac

144 ent HR and random chromosomal integration of linearized plasmid DNA, a subtype of NHR, which we demon

145 Nuclear injection of linearized plasmid DNA, circular DNA with a large gap, o

146 We also report that the degradation of linearized plasmid DNA, even devoid of chi sites, is nev

147 o-transforming Escherichia coli strains with linearized plasmid DNA, sequencing the resulting junctio

148 percoiled plasmid DNA is more sensitive than linearized plasmid DNA.

149 vity and promotes intermolecular ligation of linearized plasmid DNA.

150 Mature phage T4 DNA and linearized plasmid DNAs containing or lacking a cloned T

151 scent protein) knockdown and a dsRed-express linearized plasmid for induction to be optically analyze

152 t a single base mismatch in a 2725 base pair linearized plasmid heteroduplex.

153 First, transfection of a linearized plasmid in which expression of the reporter g

154 , EcoRI and KpnI increased the efficiency of linearized plasmid integration up to 5-fold in CHO cells

155 The o-tRNA can be transcribed either from a linearized plasmid or from a crude PCR product.

156 cat deletion by natural transformation with linearized plasmid pPAN4 (catBCIJF) 10 mutants were unab

157 tant), the predominant reaction product is a linearized plasmid resulting from single end cleavage.

158 of fission yeast with the PCR fragment and a linearized plasmid vector prepared such that the ends of

159 nsformants from a pool of PCR products and a linearized plasmid vector.

160 gonucleotides are ligated onto the ends of a linearized plasmid which is then transformed into Saccha

161 We used recircularization of a linearized plasmid with 3-P-blocked termini, mimicking t

162 ded templates were generated by digestion of linearized plasmid with the double-strand-specific T7 ge

163 and RecD leads to processive unwinding of a linearized plasmid.

164 ecific with both a 272 bp DNA fragment and a linearized plasmid.

165 g hairpin ends that is not seen in repair of linearized plasmids and may be involved in positioning t

166 Small linearized plasmids appeared to be inserted into the nuc

167 ed capacity for correct recircularization of linearized plasmids by illegitimate end-joining after tr

168 Linearized plasmids carrying a Saccharomyces cerevisiae

169 d with various concentrations of circular or linearized plasmids containing different lengths of the

170 pacity of cells to recircularize transformed linearized plasmids correctly by non-homologous DNA end

171 We transformed yeast with a series of linearized plasmids to examine the role of Pol4 (Pol IV,

172 plementation groups A, C, D2, and G rejoined linearized plasmids with a significantly decreased effic

173 ScaI-linearized plasmids with single (pBS+) and double (pGEM-

174 s were prepared by in vitro transcription of linearized plasmids with T7 RNA polymerase.

175 id being supercoiled and was not observed in linearized plasmids, consistent with the presence of the

176 of LigD in vivo only mildly impairs NHEJ of linearized plasmids, indicating that an additional DNA l

177 s and N. gonorrhoeae, using serially diluted linearized plasmids.

178 nd in situ; on duplex DNA in supercoiled and linearized plasmids; and on oligonucleotide DNA/DNA and

179 Linearized plots of the percentages of (4)C1 against lim

180 using the finite difference solution to the linearized Poisson-Boltzmann equation (FDPB) and solvati

181 sed on the finite difference solution to the linearized Poisson-Boltzmann equation reproduce the obse

182 Calculations using the linearized Poisson-Boltzmann equation show that the effe

183 approximation (RPA), in combination with the linearized Poisson-Boltzmann equation to describe solvat

184 We coupled the RPA framework with the linearized Poisson-Boltzmann equation to model solvation

185 A solution of the linearized Poisson-Boltzmann equation, for this system,

186 With finite difference solutions of the linearized Poisson-Boltzmann equation, we then calculate

187 y-combining site are computed by solving the linearized Poisson-Boltzmann equation.

188 merical and model dependent solutions of the linearized Poisson-Boltzmann equation.

189 uation approach is applied to discretize the linearized Poisson-Boltzmann equation; the resulting int

190 g: 1), full nonlinear Poisson-Boltzmann; 2), linearized Poisson-Boltzmann; and 3), neglect of solute

191 When linearized pPAC-ResQ vector and P1 or PAC DNA are cotran

192 Starting with a solution of the linearized problem about the flat rest state, a family o

193 ooperate in alginate degradation with BeKdgF linearizing producing 4,5-unsaturated monouronates.

194 y to recover accurate de novo assignments to linearized products was critically tested using performa

195 Linearized pUC19 plasmid was ligated to an oligomer cont

196 spite drug cessation (P = .03) with a median linearized rate of change of 0.42 mm/y.

197 The linearized rate of rejection (episodes of rejection/100

198 ion fraction > or =60% and sinus rhythm, the linearized rate of SUD was not different in functional c

199 The yearly linearized rate of sudden death was 1% in patients in fu

200 ential function The ground deformation has a linearized rate slope, i.e. acceleration, of 0.6 cm/yr(2

201 /- 2% and 18.8 +/- 4%, respectively, and the linearized rate was 1.8% per year.

202 +/- 3% and 48 +/- 6%, respectively, and the linearized rate was 5.0 +/- 0.7% per year.

203 Linearized rates (events/patient-year) were: reoperation

204 d plasmid concentration and was greater when linearized rather than circular plasmid DNA was used.

205 Linearized, recombinogenic pClasper targeting vector and

206 In this work, we propose a real-time linearized reconstruction algorithm that makes use of an

207 Therefore, the efficient transcription of linearized, relaxed templates found here demonstrates th

208 andom with DNase I, and incorporation of the linearized, repaired, blunt-ended, rearranged genes into

209 a 3'-phosphate-containing terminally gapped, linearized reporter plasmid.

210 (2) To identify how the model generates this linearized response in a nonlinear network.

211 mentation scheme rooted in the principles of linearizing response surfaces is introduced, which signi

212 This interaction produces linearized responses that agree with both extracellular

213 on can alleviate these effects but typically linearizes responses and reduces amplification gain.

214 The resulting linearized seco acid does not trigger a cytosolic Ca(2+)

215 of cAMP without the limitation of requiring linearized signals imposed by the 1D approach.

216 single (18)F-fallypride PET protocol and the linearized simplified reference region kinetic model in

217 ine release (gamma) was quantified using the linearized simplified reference region model contrasting

218 PET data were analyzed using the linearized simplified reference region model, which acco

219 st NXS2 neuroblastoma by gene therapy with a linearized, single-chain (sc) version of the heterodimer

220 tissue-compartment graphical analysis with 6 linearized solutions of the fundamental binding equation

221 eir complements, creating a cascade in which linearized species accumulate exponentially.

222 ion and van der Waals forces is studied as a linearized stability problem in a purely irrotational an

223 race/ethnicity, and year, with Taylor series linearized standard errors clustered by state and classr

224 ty delivery, accounting for clustering using linearized standard errors.

225 e problem, which is converted to a family of linearized subequations.

226 reactions were performed with the bleomycin-linearized substrate.

227 hat blockade of transient potassium channels linearizes summation.

228 The linearized system depends upon Froude numbers reflecting

229 The mathematical model used here is the linearized system of differential equations introduced i

230 romyces cerevisiae between genomic DNA and a linearized TAR cloning vector containing targeting seque

231 promoter template was greater than that on a linearized template.

232 emonstrate that the resulting changes to the linearized temporal filters of this nonlinear network ma

233 a large membrane-bound protein (214 kDa when linearized) that mediates internalization of vitellogeni

234 lusterize, sorts sequences by relatedness to linearize the clustering problem.

235 such as A-type potassium currents, which can linearize the frequency-input current curve.

236 e DNA minus strand at specific sites and can linearize the minus strand via a non-homologous recombin

237 t that eukaryotic topoisomerase I (top1) can linearize the open circular DNA of duck hepatitis B viru

238 After logarithmic transformations to linearize the secretion and sensitivity measures, the in

239 ction value" (p) setting that can be used to linearize the signal through digital signal processing.

240 t layer 5) or the thalamic reticular nucleus linearized the cortical responses, suggesting that layer

241 5-HT(3) receptor agonist-induced responses, linearized the current-voltage relationship, increased a

242 Human auditory efferent activation linearizes the cochlear response for mid-level sounds wh

243 asing the extracellular Ca(2+) concentration linearizes the current-voltage curve of the proctolin-in

244 n of [K(+) ](c) or chelation of [Ca(2+) ](c) linearizes the G(K(LV)) steady-state I-V curve, consiste

245 n of [K(+) ](c) or chelation of [Ca(2+) ](c) linearizes the G(K(LV)) steady-state I-V curve, suggesti

246 mation of a calcium-carboxylate network that linearizes the helix and exposes the hydrophobic amino a

247 over an invertible coordinate transform that linearizes the nonlinear BVP and identifies both a linea

248 ctivity with subthreshold depolarization and linearizes the relationship between synaptic input and n

249 ultraphosphates and discover a rearrangement linearizing the branched structure.

250 t the helper bacteria inactivate tolaasin by linearizing the lipocyclopeptide.

251 stem cells, which can be partly activated by linearizing the nano-blockers.

252 responding system of differential equations, linearizing the system about the steady state, and deter

253 Achieving high speed and long range requires linearizing the VCSEL wavenumber sweep to efficiently ut

254 r reduced activity, but when the plasmid was linearized, the enhancer-blocking activity was observed

255 do not block gene expression until they are linearized through the application of an external trigge

256 ation near the membrane by extrapolating the linearized time dependencies of selectivity coefficients

257 ption reaction in which products are readily linearized to permit regiodefined alkene dicarbofunction

258 in S. cerevisiae in which the rDNA axis was "linearized" to reduce its dimensionality, thereby reveal

259 A-seq normalization approach, Count based on Linearized Transcriptome Size (CLTS), which corrects dif

260 Here, we introduce SEQUOIA, a linearized transformer model that predicts cancer transc

261 by simple crossings-over between ends of the linearized transforming fragment and the target chromoso

262 ydrolyze the trilactone just once to produce linearized trimers.

263 dependence of Hb absorbance was effectively linearized using the current technique (R(2) = 0.990+/-0

264 were constructed by co-transformation with a linearized vector and a PCR-synthesized hoc gene.

265 When mixed together, the linearized vector and PCR products directionally assembl

266 vivo recombination between genomic DNA and a linearized vector containing homologous sequences, or ho

267 d affinity capture (TAC) for purification of linearized vector DNA, and (2) universal primer sequence

268 Electroporation with linearized vector gave approx. 10(6) transformants per m

269 triction enzyme is electroporated along with linearized vector into cells, thus determining the site

270 nction sites reveals that integration of the linearized vector occurred with minimal rearrangement of

271 onstructed in vivo by co-transformation with linearized vector plus insert DNAs, followed by intermol

272 piece of gel, and the gel extraction of the linearized vector.

273 long with two genetically marked (M1 and M2) linearized vectors that contained a human Alu sequence a

274 iscoelastic sample have been computed with a linearized version of the lattice Boltzmann method (LBM)

275 ing nearly two hundred oligonucleotides to a linearized version of the m13mp18 virus genome.

276 eling, particularly those based on classical linearized viscoelasticity.

277 ds, in its entirety, on the structure of the linearized wave equation.

278 cular, supercoiled DNA vector pUC19 is first linearized with a restriction enzyme to create a sequenc

279 tions were crosslinked with psoralen and UV, linearized with restriction enzymes and spread for EM vi

280 S1nuclease treatment of replication products linearized with single cutting restriction enzymes, foll

281 ing intensity is greater when the plasmid is linearized with the TRS at an end (39-49%) than when lin

282 ed with the TRS at an end (39-49%) than when linearized with the TRS in the interior (18-22%).

283 PCR-amplified with 30-40 bp of homology to a linearized yeast plasmid.

284 used for rapid and efficient cloning into a linearized yeast vector that contains these same element