ライフサイエンスコーパス: locked nucleic acid

1 l elements of 2'-O-methoxyethyl residues and locked nucleic acid.

2 time that it is possible to obtain LNA-like (Locked Nucleic Acid 1) binding affinity and biological a

3 loops, CNG repeats, dangling ends, inosines, locked nucleic acids, 2-hydroxyadenines and azobenzenes.

4 Here, we show that antigene locked nucleic acids (agLNAs) reduce RNA levels of targe

5 nse oligonucleotides composed exclusively of locked nucleic acids (all-LNAs) complementary to CUG rep

6 on duplexes containing targeted insertion of locked nucleic acid analogs and abasic lesions in either

7 Locked nucleic acid analogs, which constrain sugar ring

8 2'-O-methyl oligonucleotide probes with LNA (locked nucleic acid) and 2,6-diaminopurine substitutions

9 (that express high levels of mir-29) with a locked-nucleic acid antagonist of mir-29b increased Mcl-

10 In the present study, we show that an 8-mer locked nucleic acid anti-miR-155 oligonucleotide targeti

11 , we observed that in vivo administration of locked nucleic acid anti-miR-181b retarded both the deve

12 d-type mice pretreated intracolonically with locked nucleic acid anti-miR-210.

13 Treatment with locked nucleic acid anti-miR-29a significantly improved

14 In vivo administration of locked nucleic acid anti-miR-29b greatly increased colla

15 ssion, but transfection with let-7f-specific locked nucleic acid anti-miRNA increased beta(2)AR expre

16 Moreover, locked nucleic acid anti-miRNA inhibitors of these six m

17 nous administration of an miR-26a inhibitor, locked nucleic acid-anti-miR-26a, increased SMAD1 expres

18 s9, or primary FLT3-ITD(+) AML samples using locked nucleic acid antisense inhibitors, results in an

19 PGDS transcript levels, the responses of the locked nucleic acid antisense oligo-treated animals from

20 We report that peptide nucleic acid and locked nucleic acid antisense oligomers that target CAG

21 ational evidence for an optimized, synthetic locked nucleic acid antisense oligonucleotide inhibitor

22 thesis by intracisternal administration of a locked nucleic acid antisense oligonucleotide to young-a

23 ock-down of survivin using EZN-3042, a novel locked nucleic acid antisense oligonucleotide, in combin

24 strategy targeting the SARS-CoV-2 RNA using locked nucleic acid antisense oligonucleotides (LNA ASOs

25 Finally, miR-29b blockade by locked nucleic acid antisense oligonucleotides prevented

26 ver, in the medial amygdala of female mice, "locked nucleic acid" antisense (AS) oligonucleotides wit

27 njection of mice on a western-type diet with locked nucleic acid-antisense oligonucleotides results i

28 Conversely, locked nucleic acid-based anti-miR-34a treatment diminis

29 d a selective inhibitory PCR (siPCR) using a locked nucleic acid-based PCR blocker to selectively inh

30 Expression of miRNA was determined by locked nucleic acid-based quantitative real-time polymer

31 Oligonucleotides containing locked nucleic acid bases (LNAs) have increased affinity

32 Essentially, this new design adds locked nucleic acid bases (LNAs) to the beacon structure

33 ese results demonstrate the potential of the locked nucleic acid bases for nucleic acid design for su

34 ere analyzed by incorporating a gold nanorod-locked nucleic acid biosensor.

35 Bis-locked nucleic acids (bisLNAs) are clamp-forming oligonu

36 say sensitivity through the incorporation of locked nucleic acid chemistries.

37 Addition of an OMe G-clamp to a 12mer OMe-locked nucleic acid chimera maintained, but did not enha

38 real-time polymerase chain reaction based on locked nucleic acid-containing primers in 174 patients,

39 Pyrene-functionalized 2'-amino-alpha-L-LNAs (locked nucleic acids) display extraordinary affinity tow

40 Here, we report a double-stranded locked nucleic acid (dsLNA) biosensor for investigating

41 d virus vectors and inhibited with antisense locked nucleic acid-GapmeRs to examine its function.

42 LNA (locked nucleic acids, i.e. oligonucleotides with a methy

43 cal imaging is performed using high-affinity locked nucleic acid imaging probes that stably yet rever

44 ram syntheses of d- or l-nucleoside analogs, locked nucleic acids, iminonucleosides, and C2'- and C4'

45 of a subset of these genes was tested using locked nucleic acid in situ analyses.

46 Moreover, blockage of miR-30b or 378 by locked nucleic acid inhibitors significantly attenuated

47 ) and miR-221 (novel in T lymphocytes) using locked nucleic acid inhibitors.

48 cing anti-GAA duplex RNAs or single-stranded locked nucleic acids into patient-derived cells increase

49 tentiation by the gapmer ASOs was blunted by locked nucleic acid (LNA) and 2'-methoxyethyl (2'MOE) mo

50 difications such as constrained ethyl (cEt), locked nucleic acid (LNA) and 2'-O-methoxyethyl (2'-MOE)

51 Locked nucleic acid (LNA) and its diastereomer alpha-L-L

52 formationally restricted nucleotides such as locked nucleic acid (LNA) are very popular as affinity-,

53 To take full advantage of locked nucleic acid (LNA) based molecular beacons (LNA-M

54 his focused library of MYCASOs incorporating locked nucleic acid (LNA) bases at the 5'- and 3'-ends,

55 Oligonucleotides that contain locked nucleic acid (LNA) bases have remarkably high aff

56 The introduction of locked nucleic acid (LNA) bases into oligonucleotides co

57 stilbene donor-acceptor capped hairpin with locked nucleic acid (LNA) bases on the dynamics and effi

58 First, locked nucleic acid (LNA) bases were incorporated into t

59 Locked Nucleic Acid (LNA) can enhance oligonucleotide af

60 ntaining bicylic modifications (BNA) such as locked nucleic acid (LNA) designed to induce target RNA

61 he (-) DNA template by inserting overlapping locked nucleic acid (LNA) doublets and triplets.

62 f VR, and alteration of TERRA levels using a locked nucleic acid (LNA) GapmeR led to large nuclear VR

63 Described herein, a Locked Nucleic Acid (LNA) Hybridization-Ligation ECL ELI

64 Oligonucleotide probes containing locked nucleic acid (LNA) hybridize to complementary sin

65 s study we show by in vivo administration of locked nucleic acid (LNA) inhibitors that suppression of

66 Locked nucleic acid (LNA) is one of the most useful modi

67 Locked nucleic acid (LNA) microarrays were used to inves

68 ning either 2'-O-methoxyethylribose (MOE) or locked nucleic acid (LNA) modifications were designed to

69 The DNA-BCA system contains a locked nucleic acid (LNA) modified DNA probe for improvi

70 that the 2'-N-(pyren-1-yl)carbonyl-2'-amino locked nucleic acid (LNA) monomer X is a highly versatil

71 entary road controlled by affinity-enhancing locked nucleic acid (LNA) monomers and additional regula

72 formationally restricted nucleotides such as locked nucleic acid (LNA) monomers are used extensively

73 nd TC-motif TFOs modified with six different locked nucleic acid (LNA) monomers, i.e. conventional an

74 Two types of chemical modification, locked nucleic acid (LNA) N(7)-methylguanosine modificat

75 Using a dual double-stranded locked nucleic acid (LNA) nanobiosensor that tracks long

76 tural elements of 2'O-methoxyethyl (MOE) and locked nucleic acid (LNA) nucleosides yielded a series o

77 introduction of 2'-deoxy-2'-fluorouridine or locked nucleic acid (LNA) nucleotides.

78 is, we investigate the use of functionalized locked nucleic acid (LNA) oligomers as biomolecular hand

79 hat antisense peptide nucleic acid (PNA) and locked nucleic acid (LNA) oligomers can bind key IRES se

80 In WTB-PCR, a nonextendable locked nucleic acid (LNA) oligonucleotide binds tightly

81 ocalization of small RNAs in plants by using locked nucleic acid (LNA) oligonucleotide probes.

82 wild-type BRAF is competitively blocked by a locked nucleic acid (LNA) oligonucleotide.

83 Conjugates containing DNA and locked nucleic acid (LNA) oligonucleotides are active, a

84 Transfection of locked nucleic acid (LNA) oligonucleotides designed to b

85 331F variant in either 2'-O-Methyl (2'-OMe), locked nucleic acid (LNA) or 2'-O-methoxy ethyl (MOE) ch

86 Optimum results were obtained with locked nucleic acid (LNA) phosphorothioate gap-mers.

87 rate detection of BRAF V600E variation using locked nucleic acid (LNA) probe-based droplet digital po

88 pography (DNA-PAINT), and the specificity of locked nucleic acid (LNA) probes for the in situ detecti

89 We also demonstrated that locked nucleic acid (LNA) probes improved sensitivity ap

90 ntaining mutations at the targeted loci, and locked nucleic acid (LNA) probes were used to enhance th

91 re based on robust digoxigenin (DIG) RNA and locked nucleic acid (LNA) probes, respectively.

92 Use of locked nucleic acid (LNA) residues in the DNA probes res

93 The influence of locked nucleic acid (LNA) residues on the thermodynamic

94 nucleotide containing 7xOMe and 5x5-methyl C locked nucleic acid (LNA) residues, and a peptide nuclei

95 ose modifications (e.g. 4'-C-methylation and Locked Nucleic Acid (LNA) substitution) at specific posi

96 ibitors containing optimized PS patterns and locked nucleic acid (LNA) sugar modifications, we charac

97 Locked nucleic acid (LNA), a conformationally restricted

98 A PSL2-targeting locked nucleic acid (LNA), administered 3 d after, or 14

99 e of primer chemistries comprising DNA, RNA, locked nucleic acid (LNA), or 2'O-methyl-DNA.

100 nucleosides in unlocked nucleic acid (UNA), locked nucleic acid (LNA), or beta-l-RNA series was eval

101 hereas inhibition of the let-7 family with a locked nucleic acid (LNA)-anti-miR has the opposite effe

102 With the use of locked nucleic acid (LNA)-antimiR-21 oligonucleotides, b

103 Locked nucleic acid (LNA)-based miRNA array was employed

104 nhanced RIC exploits the stronger binding of locked nucleic acid (LNA)-containing oligo(dT) probes to

105 rmine lncRNA localization using biotinylated locked nucleic acid (LNA)-containing oligonucleotides wi

106 molecular beacons with DNA, RNA and combined locked nucleic acid (LNA)-DNA backbones can all detect m

107 s two spectrally distinguishable fluorescent locked nucleic acid (LNA)-DNA oligonucleotide probes to

108 amage in porcine cardiac tissues and whether locked nucleic acid (LNA)-modified anti-miR chemistries

109 herapeutic potential of 8-mer seed-targeting locked nucleic acid (LNA)-modified anti-miR oligonucleot

110 using an s.c.-delivered seed-targeting 8-mer locked nucleic acid (LNA)-modified antimiR (LNA-antimiR-

111 Here, we determined whether locked nucleic acid (LNA)-modified antisense oligonucleo

112 gets the liver-expressed miRNA-122 using the locked nucleic acid (LNA)-modified antisense oligonucleo

113 In the present study, the locked nucleic acid (LNA)-modified cap analogue 3, m(7(L

114 We evaluated the efficacy of a locked nucleic acid (LNA)-modified inhibitor of miR-192,

115 )-ribose, or 2'-O, 4'-C-methylene bridged or locked nucleic acid (LNA)-modified nucleotides show subs

116 t of chronically infected chimpanzees with a locked nucleic acid (LNA)-modified oligonucleotide (SPC3

117 essenger RNA (mRNA) in suspended cells using locked nucleic acid (LNA)-modified oligonucleotide probe

118 ded (FFPE) tissues and cultured cells, using locked nucleic acid (LNA)-modified oligonucleotides.

119 the IL-6-deficient (IL-6((-)/(-))) mice with locked nucleic acid (LNA)-modified phosphorothioate olig

120 emonstrate efficient delivery using a simple locked nucleic acid (LNA)-polymer conjugate that assembl

121 estricted nucleotide building blocks such as locked nucleic acid (LNA).

122 Here, we established a double-stranded locked nucleic acid (LNA)/DNA (LNA/DNA) nanobiosensor fo

123 DNA/locked nucleic acids (LNA) AMOs synthesized with a phosp

124 G)-labeled oligonucleotide probes containing locked nucleic acids (LNA) and 1-ethyl-3-(3-dimethylamin

125 We investigate the combination of locked nucleic acids (LNA) and charge neutral backbones

126 we investigated the disruption of G4s using Locked Nucleic Acids (LNA) as invader probes.

127 combination of site-directed mutagenesis and locked nucleic acids (LNA) complementary to defined doma

128 ation of TD05 coupled with modification with locked nucleic acids (LNA) increased conformational stab

129 Locked nucleic acids (LNA) show remarkable affinity and

130 We have used locked nucleic acids (LNA) to engineer novel molecular b

131 hemical modifications such as 2'-O-Me, 2'-F, locked nucleic acids (LNA), unlocked nucleic acids (UNA)

132 We modify the magnetic beads with locked nucleic acids (LNA), which have high affinity and

133 ng phosphorodiamidate morpholino oligomer or locked nucleic acids (LNA)/2'-OMe mixmers with different

134 that Hsp90 protein binds PS-ASOs containing locked-nucleic-acid (LNA) or constrained-ethyl-bicyclic-

135 species-specific electrochemical DNA probe (locked nucleic acid, LNA) was synthesized and implemente

136 Inhibition of miR-30 using complementary locked nucleic acids (LNA30bcd) in both human IECs and h

137 However, the recent additions to the field - locked nucleic acids (LNAs) and peptide nucleic acids (P

138 Locked nucleic acids (LNAs) are synthetic nucleic acid a

139 e the potential of novel antigens containing locked nucleic acids (LNAs) as targets for antibodies.

140 linkages and also 2'-OMe ribonucleotides and locked nucleic acids (LNAs) at selected sites.

141 Here, we describe a unique use of locked nucleic acids (LNAs) for studying nuclear long no

142 ioate antisense oligonucleotides (ASOs) with locked nucleic acids (LNAs) improve target affinity, RNa

143 The presence of locked nucleic acids (LNAs) in the ssODNs at mismatching

144 Therapeutic delivery of locked nucleic acids (LNAs) targeting BCAR4 attenuated Y

145 breast cancers, and therapeutic delivery of locked nucleic acids (LNAs) targeting BCAR4 strongly sup

146 Treatment of cells with locked nucleic acids (LNAs) targeting these miRNAs inhib

147 bility of oligonucleotides (ODNs) containing locked nucleic acids (LNAs) to bind supercoiled, double-

148 he most potent siRNA, siR-7, was modified by Locked nucleic acids (LNAs) to obtain siR-7-EM with incr

149 ctive targeting ss(ATTCC)(n) using antisense locked nucleic acids (LNAs), effectively disrupting NET-

150 In 2005, we introduced Invader locked nucleic acids (LNAs), i.e., double-stranded probe

151 d gain-of-function studies, using anti-sense locked-nucleic acids (LNAs) and synthetic RNA mimetics,

152 High-throughput screening (HTS) of 997 locked nucleic acid miRNA inhibitors was performed in si

153 led miRNAs by next generation sequencing and locked nucleic acid miRNA microarrays and verified conco

154 The strong northern-biased locked-nucleic acid modification exacerbated the negativ

155 of miR-21 through intravenous delivery of a locked nucleic acid-modified (LNA-modified) antimiR olig

156 Blocking endogenous miR-140 by locked nucleic acid-modified anti-miR partially sensitiz

157 A locked nucleic acid-modified anti-miR-146a given before

158 Inhibition of miR-23 and miR-27 function by locked nucleic acid-modified anti-miRNAs represses angio

159 n of the miR-15 family in neonatal mice with locked nucleic acid-modified anti-miRNAs was associated

160 iac remodeling, our current study employed a locked nucleic acid-modified antimiR to target miR-378 i

161 Locked nucleic acid-modified antisense miR-92a (LNA-92a)

162 In Ang II-infused mice, locked nucleic acid-modified antisense miR-92a (LNA-miR-

163 Here, we have shown that a locked nucleic acid-modified antisense oligonucleotide c

164 cardiac mRNA for therapeutic purposes using locked nucleic acid-modified antisense oligonucleotides.

165 ction, and extracellular matrix genes, using locked nucleic acid-modified antisense TFIIB oligonucleo

166 Miravirsen is a locked nucleic acid-modified DNA phosphorothioate antise

167 Targeting miR-192 with locked nucleic acid-modified inhibitors in vivo decrease

168 diac disease models were administered with a locked nucleic acid-modified oligonucleotide (LNA-antimi

169 The simple systemic delivery of locked nucleic acid-modified, anti-miR-29b-reduced endog

170 miR-21 inhibition using locked-nucleic-acid-modified oligonucleotide effectively

171 isense oligonucleotides (oligos), containing locked nucleic acid moieties (an improved technology), t

172 To optimize beacons for RT-LAMP, multiple locked nucleic acid monomers were incorporated to elevat

173 A gold nanorod-locked nucleic acid nano-biosensor for dynamic single-ce

174 In situ hybridization analysis, using locked nucleic acid oligo probes complementary to the n1

175 Kdm6b small interfering RNA or an antisense locked nucleic acid oligonucleotide specific to Arc eRNA

176 ibers, we blocked miR-210 with complementary locked nucleic acid oligonucleotides (anti-miR-210).

177 bilateral intraamygdala injections of OTR AS locked nucleic acid oligonucleotides several days before

178 ymine, is weakly hybridized to complementary locked nucleic acid or 2'-OMe RNA ONs that are also subs

179 2', 4'-bridged nucleic acids (also known as locked nucleic acid or LNA, and 2',4'-constrained ethyl

180 Blocking miR-146a with locked nucleic acids or antago-miRs attenuated PPCM in C

181 Treatment with LINK-A locked nucleic acids or GPCR antagonists stabilized the

182 properties were optimized using 2'-O-methyl, locked nucleic acid, or phosphorodiamidate morpholino ba

183 es; and DNA backbone modifications including locked nucleic acids, peptide nucleic acids and borane n

184 Using a locked nucleic acid primer specific for > or =6 AT repea

185 paraffin-embedded tissues were hybridized to locked nucleic acid probes against 752 human miRNAs (rep

186 The use of locked nucleic acid probes and antibodies conjugated wit

187 ned optimal hybridization parameters for 130 locked nucleic acid probes by recording nucleic acid mel

188 We used locked nucleic acid probes for in situ hybridization ana

189 We used locked nucleic acid probes in in situ hybridisation reac

190 develop and validate a qPCR assay utilizing locked nucleic acid probes to detect different mitotypes

191 ombines the unique recognition properties of locked nucleic acid probes with enzyme-labeled fluoresce

192 Using hybridization with locked nucleic acid probes, we directly detected abortiv

193 As is achieved by in situ hybridization with locked nucleic acid probes.

194 cent in situ hybridization (flow-FISH) using locked-nucleic acid probes is combined with rolling circ

195 ilencing of miR-125b by systemic delivery of locked nucleic acid rescued angiotensin II-induced periv

196 T(L)AC(L)GA(L)CG(L)GC(L)C, with L denoting a locked nucleic acid residue, inhibit 50% of group I intr

197 Oligonucleotides composed of 2'-O-methyl and locked nucleic acid residues complementary to HIV-1 tran

198 ice, inhibition of miR-193a by complementary locked nucleic acids resulted in an upregulation of the

199 Control of ground-state conformation by the locked nucleic acids results in a marked increase in bot

200 We developed a locked nucleic acid reverse transcription real-time PCR

201 Magnetic beads with a biotinylated locked nucleic acid sequence capture target sequences wi

202 inhibition of miR-30a-5p in the mPFC using a Locked Nucleic Acid sequence that targets miR-30a-5p res

203 reatment of PNET cells with Erk inhibitor or locked nucleic acids sequestering miR-431 inhibits invas

204 Targeting of HSATII with antisense locked nucleic acids stimulated IFN response and induced

205 Purification protocols using oligo dT's, locked nucleic acid substituted dT's, and tetramethylamm

206 l agRNAs can contain 2'F-RNA, 2'OMe-RNA, and locked nucleic acid substitutions, or combinations of mu

207 Consistent with this notion, let-7f locked nucleic acid transfection caused depressed agonis

208 nd duplex DNA structures possessing a single locked nucleic acid TT step are reported.

209 titive antagonism of TLR8 with non-targeting locked nucleic acids was found to prevent native LDL-ind

210 Using structure-disrupting, antisense locked nucleic acids, we then demonstrate that the WNV g