ライフサイエンスコーパス: low-affinity binding

1 h affinity binding while having no effect on low affinity binding.

2 Transfection of TSLPR cDNA resulted in only low affinity binding.

3 a second estimate of 1.4 microM was made for low-affinity binding.

4 there a phenotype-specific downregulation of low-affinity binding.

5 stroyed norepinephrine neurons and abolished low-affinity binding.

6 ous dopamine neurons and selectivity ablated low-affinity binding.

7 eural agrin to intact myotubes revealed only low-affinity binding.

8 other phosphorylated sequences that exhibit low-affinity binding.

9 tions to hydration release in both high- and low-affinity binding.

10 unts for matrix:cell volume ratio, high- and low-affinity binding, activity coefficients, background

11 Since alpha(1)-antagonists display low affinity binding at beta(2)-adrenergic receptors, tw

12 g a possible mechanism whereby specific, yet low-affinity, binding between Nup98 and Nup96 is respons

13 Finally, destabilization of low affinity binding by salt is in quantitative agreemen

14 In addition, stabilization of the low affinity binding conformation of the C2 domain of fV

15 near the amino terminus of the KID mediates low affinity binding enhanced by phosphorylation of adja

16 r between a QD and a molecular probe to even low-affinity binding events at the QD/solvent interface.

17 These technologies must be able to identify low-affinity binding events that are often observed betw

18 One subunit, IFNAR1, has low affinity binding for interferon yet is required for

19 he drug binds are unknown, although high and low affinity binding has been reported in cardiomyocytes

20 inished nicotine-induced increased nicotinic low-affinity binding in the cerebellum and diminished di

21 potentiating effect of increasing nicotinic low-affinity binding in the frontal cortex.

22 shed dizocilpine-induced increased nicotinic low-affinity binding in the hippocampus and hypothalamus

23 veloped DIO after 3 months on HE diet, their low affinity binding increased slightly.

24 The low affinity binding is competed by nonlytic human HDL a

25 g is destabilized by osmotic stress, whereas low affinity binding is insensitive.

26 mediated by Lys(415), whereas the source of low affinity binding is unknown.

27 domain 3 alone construct is capable of only low affinity binding (K(d) approximately 500 nm) toward

28 the theory predicts that both catalytic and low-affinity binding (K(d) greater, >or= 0(-7) M) protei

29 alone resulted in a protein capable of only low affinity binding (Kd approximately 1 microm) toward

30 Addition of low-affinity binding ligands had no detectable effect on

31 cell interactions could be characterized by low-affinity binding (< or = 10(5) M(-1)) to a high numb

32 inity ADP binding mode (K(app1)) but not the low affinity binding mode (K(app2)).

33 e substrate, whereas MDDMA and MDTMA adopt a low-affinity binding mode consistent with an inhibitor,

34 ional selection mechanism contrasts with the low-affinity binding mode of 53BP1, and it ensures 53BP1

35 RovA contacts mainly the DNA backbone in a low-affinity binding mode, which allows the immediate re

36 The number of low-affinity binding motifs is significantly depressed i

37 is is achieved using multiple, minimalistic, low-affinity binding motifs that are in rapid exchange w

38 25I-EGF, the EGFR/RET chimera exhibited only low affinity binding of 125I-EGF.

39 HV-cyclin complexes in vitro indicating that low affinity binding of cyclins to the Cdk subunit might

40 These effects were mediated by direct low affinity binding of estradiol to the maxi-K channel

41 tch regions of CDC42 or RAC1 and (ii) a very low affinity binding of GRD and a C terminus adjacent to

42 the mutation minimally affected the initial low affinity binding of heparin to antithrombin, but gre

43 bdominance of the JHA 210-219 was not due to low affinity binding of JHA 210-219 to H-2Kd or to ineff

44 By contrast with TPA, low affinity binding of SAPD was inhibited by sn-1,2-dio

45 f ATP (K(d)(ATP)=5-6 microM) was followed by low affinity binding of Tide (K(d)(Tide)=180 microM), an

46 T) proteins, GSTA1 and GSTM1, exhibited only low affinity binding of xanthophylls.

47 h DnaB protein, and in unwinding of oriC, or low-affinity binding of ATP.

48 higher concentrations, which may rely on the low-affinity binding of CNTF to the IL-6R.

49 al structural changes necessary to transform low-affinity binding of dofetilide by the related bovine

50 gE is in protein aggregation diseases, where low-affinity binding of endogenous proteins is preferred

51 charge is followed by the voltage-dependent low-affinity binding of extracellular chloride ions.

52 ith the allosteric binding site manifests as low-affinity binding of methoctramine at the M(3) recept

53 litate plasma membrane targeting through the low-affinity binding of NB to phosphorylated inositol po

54 c18/SNARE interaction modes involve the same low-affinity binding of the extreme syntaxin-1 N terminu

55 the context of the holenzyme, and we detect low-affinity binding of the kinase and dsRBD constructs

56 (human numbering), which is responsible for low-affinity binding of the substrate in endo-mode.

57 a concentration-driven interaction with the low-affinity binding partner DAB2, finding that this int

58 PF recognition, thus stabilizing networks of low affinity binding partners at endocytic sites.

59 In contrast, complexes made with low affinity binding peptides were highly sensitive to d

60 Only low-affinity binding (pKI) was observed when guanine nuc

61 In this study, we identify Tau as a low-affinity binding receptor for GCs that plays a cruci

62 own to be regulated by two discrete high and low affinity binding regions for diacylglycerols and pho

63 isotherms exhibit apparent high-affinity and low-affinity binding regions, corresponding respectively

64 was associated with loss of hydration water, low-affinity binding released more hydration water.

65 A method for quantifying low-affinity binding revealed recurring antibody variabl

66 dentified to date contain almost exclusively low affinity binding sequences, our analysis indicates t

67 brin sequence is an important constituent of low affinity binding, since thrombin binding at this sit

68 binding site (Ki = 50 microM) and a second, low affinity binding site (Kii = 0.7 mM).

69 and AP2gamma resulted in the activation of a low affinity binding site construct to levels comparable

70 In this study, both a high affinity and a low affinity binding site for omega-conotoxin MVIIC were

71 ereas the phosphorylated Thr(187) provides a low affinity binding site for the Skp2/Cks1 complex.

72 constant of approximately 10(7) M-1 and the low affinity binding site has an apparent binding consta

73 ovide a structural basis for the role of the low affinity binding site in FGF receptor discrimination

74 These results indicate a second low affinity binding site in the Robo-Slit complex as we

75 The low affinity binding site is shown to be present on the

76 gand stacking are consistent with the single low affinity binding site observed for 4-CPI and 4-PP.

77 histamine differs significantly between the low affinity binding site of FS-HBP and monomine, sugges

78 e, mediated by an LXCXE motif and a separate low affinity binding site present within an activation d

79 ted that contained a high affinity G-rich or low affinity binding site.

80 ve a value that allows interactions with the low affinity binding site.

81 g site and a 27% increase in the Bmax of the low affinity binding site.

82 D(2) and A(2A) receptors are involved in the low affinity binding site.

83 and decreasing the affinity of cGMP for the low-affinity binding site (CNB-B).

84 Cdc13 truncations revealed that the low-affinity binding site (OB1), which is distal from th

85 Soluble Hb bound saturably to a low-affinity binding site [K(d) = (1.1+/-0.2) x 10(-6) M

86 bition can be explained by the presence of a low-affinity binding site and in vivo hydrolysis of NPA.

87 extends upstream, another overlaps the ToxT low-affinity binding site around the -35 element, and th

88 omers, one of which possessed a promiscuous, low-affinity binding site for aromatic ligands, includin

89 2, PDE5, and PDE6, which likely constitute a low-affinity binding site for cGMP.

90 site for etomidate and a recently disclosed low-affinity binding site for diazepam.

91 This sequence contains a low-affinity binding site for POU domain proteins, and c

92 Further evidence for a low-affinity binding site is indicated by the inhibitory

93 The association constant for this low-affinity binding site of DtxR(E175K) obtained from c

94 t AMA causes the loss of a Zn(2+) ion from a low-affinity binding site of NDM-1.

95 The single low-affinity binding site of the K324A mutant to isoprot

96 2)PKG-I beta requires binding of cGMP to the low-affinity binding site of the R domain.

97 while binding of calmodulin to the supposed low-affinity binding site on CaMKII is compatible with c

98 lity of the Katp channel suggesting that the low-affinity binding site resides on these neurons.

99 The KD for the low-affinity binding site was determined to be 1.1 micro

100 A high-affinity (0.6 x 10(-9) mmol/L) and a low-affinity binding site were present.

101 of 2 x 10(-7), binding site 2 (primary) is a low-affinity binding site with a binding constant of 6.3

102 n are mediated respectively by the high- and low-affinity binding site(s) of IKP104.

103 receptor (c-MPL) via steric occlusion of the low-affinity binding site, contributing to perturbation

104 Interestingly, an additional low-affinity binding site, mediated by a structurally an

105 modeled outward-open cleft contribute to one low-affinity binding site, whereas arginine 440 located

106 pin transition, thus revealing an additional low-affinity binding site.

107 ical interactive high-affinity sites and one low-affinity binding site.

108 giotensin IV) competed with both a high- and low-affinity binding site.

109 caused a 70-80% reduction in its binding to low affinity binding sites establishing its identity as

110 eas the EGF receptor exhibited both high and low affinity binding sites for 125I-EGF, the EGFR/RET ch

111 Relatively low affinity binding sites for monosaccharides are forme

112 >90% of TFPI bound to a single population of low affinity binding sites on hepatoma cells (2 x 10(6)

113 difference being due to the contribution of low affinity binding sites on the RRE.

114 But DIO-prone rats had few or no low affinity binding sites throughout the forebrain.

115 unt of weight as chow-fed controls but their low affinity binding sites were reduced to DIO levels an

116 inding sites, but also numerous moderate and low affinity binding sites, are under negative selection

117 ionic channel activity at the phospholipids low affinity binding sites, thus indicating that SecA is

118 he overall enhancer architecture-clusters of low affinity binding sites-is maintained and required fo

119 nding domain and its complexes with high and low affinity binding sites.

120 one high affinity binding site and multiple low affinity binding sites.

121 eoglycans (HSPGs) may be responsible for the low affinity binding sites.

122 ns with Trk A also resulted in predominantly low affinity binding sites.

123 bind approximately 1.6 thrombin molecules at low-affinity binding sites (Kd = 2.8 muM) and approximat

124 igh-affinity (mAb 247) or both the high- and low-affinity binding sites (mAb 370 and mAb 387).

125 vival oncogene MITF as a model, we show that low-affinity binding sites act as a competitive reservoi

126 helial cells by enhancing binding of VEGF to low-affinity binding sites and neuropilin-1 and stimulat

127 Here, we find that suboptimal or low-affinity binding sites are necessary for precise gen

128 conversion of CVB that include the high- and low-affinity binding sites associated with capsid breath

129 he relative proportions of high-affinity and low-affinity binding sites did not change.

130 nt receptors that had GTP-insensitive single low-affinity binding sites for agonists and reduced pote

131 We conclude that band 4.2 provides low-affinity binding sites for both band 3 oligomers and

132 ibited one high-affinity binding and several low-affinity binding sites for cis-parinaroyl-CoA, a nat

133 r, we proposed that there may be high- and a low-affinity binding sites for the BvgA dimer.

134 r the long-standing observation of high- and low-affinity binding sites for the prototypic inhibitor

135 cription factor Ultrabithorax (Ubx) utilizes low-affinity binding sites in the Drosophila melanogaste

136 ast, ADP occupies one high-affinity and five low-affinity binding sites in vitro, consistent with con

137 endent sites with the apparent population of low-affinity binding sites increasing with the size of t

138 d state are consistent with its occupancy of low-affinity binding sites necessary for promoter functi

139 ation could be due to release of band 3 from low-affinity binding sites on ankyrin.

140 iso-1-cytochrome c (yCc) with the high- and low-affinity binding sites on cytochrome c peroxidase co

141 ing studies suggest coexistence of high- and low-affinity binding sites or negative cooperativity.

142 pM and 8 nM, corresponding to the high- and low-affinity binding sites seen on phytohemagglutinin-ac

143 ochord enhancers, including those containing low-affinity binding sites that would be excluded by sta

144 into the brain parenchyma, where it bypassed low-affinity binding sites that would otherwise sequeste

145 r Irf4 abundance with its recruitment toward low-affinity binding sites within Teff cell cis-regulato

146 ays show that RRE stem-loop II has high- and low-affinity binding sites, each of which binds a Rev di

147 ical RNA-binding domain to recognize several low-affinity binding sites, including the well-character

148 nd binding to GPCRs have revealed transient, low-affinity binding sites, termed metastable binding si

149 inhibition by the NTD and to compensate for low-affinity binding sites, thereby allowing for full oc

150 how that there are two high-affinity and two low-affinity binding sites, to which the binding of cAMP

151 uffer from the inability to model and detect low-affinity binding sites, which have recently been sho

152 sotherms of [3H]8-OH-DPAT revealed high- and low-affinity binding sites.

153 UGT1A10-His or UGT1A7-His revealed high- and low-affinity binding sites.

154 es on HPASMCs, yet BMP2 competes only at the low-affinity binding sites.

155 rectly binds the flhDC promoter at high- and low-affinity binding sites.

156 rent K(d) values of 1.86 and 71.2 nm for the low-affinity binding sites.

157 nes and developed a species of specific, but low-affinity, binding sites for fMLP, in association wit

158 and catalytic domain (152-528), whereas the low-affinity binding state only requires residues in the

159 r (e.g., angiotensin IV) were shifted toward low-affinity binding states following peptide metabolism

160 finity binding to mispair-containing DNA and low affinity binding to fully base-paired DNA both invol

161 nduced cellular responses, initiated through low affinity binding to gp130, are mediated by two heter

162 (KD = 0.83 muM) compared to G7-B1 and shows low affinity binding to Grb2-, Grb10- and Grb14-SH2 doma

163 yrosine kinase receptors in conjunction with low affinity binding to heparin sulfate proteoglycans.

164 0 nM [3H]ryanodine reveal saturable high and low affinity binding to membrane preparations from wild-

165 nal residues, including F47, are involved in low affinity binding to MHC class II alpha-chain.

166 Low affinity binding to mono- and disulfated GSPs is lar

167 ow high affinity binding to site I, but only low affinity binding to site II, show that retardation o

168 Surprisingly, despite its low affinity binding to the isolated CNBD, cAMP also had

169 sion of DBP1 may have been selected to allow low-affinity binding to another receptor on Duffy-null e

170 tissue homogenates) had minimal influence on low-affinity binding to AT(1) and AT(2) receptors, yet c

171 altering glucose availability upon high- and low-affinity binding to brain SUR.

172 Domain 4 exhibited low-affinity binding to LRP6 in in vitro binding assays,

173 may bind overlapping genomic regions due to low-affinity binding to other sequence motifs.

174 to the chaperone-adhesin (PapDG) complex and low-affinity binding to the major tip subunit PapE (PapD

175 toire was depleted of TCRs that demonstrated low-affinity binding to their ligands.

176 Yeast GAPDH and aldolase showed low-affinity binding to yeast actin, which suggests that

177 ate-binding modules (CBMs) to compensate for low-affinity binding typical of protein-carbohydrate int

178 APD binding to its high affinity site, while low affinity binding was unaffected.

179 h avoided the inherent problem of relatively low affinity binding which hindered the use of eukaryoti

180 It may prevent nonspecific low-affinity binding while keeping the site available fo

181 otif in its cytoplasmic domain that mediates low affinity binding with the endocytic adaptor protein

182 proximately 3 pmol/mg total cell protein and low-affinity binding with a Kd of approximately 1.6 micr

183 t the RNA-binding surface allow for high- or low-affinity binding with functional implications.