ライフサイエンスコーパス: mRNA export

1 to define how these factors control Dbp5 and mRNA export.

2 gene expression including transcription and mRNA export.

3 adaptors that couple pre-mRNA processing to mRNA export.

4 expression but did not alter Rex-3-dependent mRNA export.

5 cts their expression upstream of its role in mRNA export.

6 on to its more broadly characterized role in mRNA export.

7 ts gene-NPC interactions, transcription, and mRNA export.

8 nuclear import is separate from its role in mRNA export.

9 lasmic face of NPCs, is crucial to terminate mRNA export.

10 and the hGle1 role in SGs is independent of mRNA export.

11 ased splicing factor mobility, and defective mRNA export.

12 is involved in transcription elongation and mRNA export.

13 d is involved in promoting transcription and mRNA export.

14 with Aly/REF contributes to efficient viral mRNA export.

15 rize the mechanism underlying IPMK-regulated mRNA export.

16 es mRNA export, RCF1 does not play a role in mRNA export.

17 lay (about 16 min in the mouse) being due to mRNA export.

18 t cells to investigate how Mex67 facilitates mRNA export.

19 -mRNA processing such as polyadenylation and mRNA export.

20 lowed a refinement of the current models for mRNA export.

21 (matrix) protein, another viral inhibitor of mRNA export.

22 epletion of any of these components inhibits mRNA export.

23 links transcription/processing with nuclear mRNA export.

24 P27 is a shuttling protein involved in viral mRNA export.

25 onents of the TREX complex, enhances histone mRNA export.

26 ell nucleus, indicating that NPM1 influences mRNA export.

27 nting the splicing requirement for efficient mRNA export.

28 ogenic stresses, leading to an inhibition of mRNA export.

29 targeting of the export factors and promotes mRNA export.

30 a double mutant is temperature-sensitive for mRNA export.

31 ene expression and coupling translation with mRNA export.

32 that is required for herpes simplex virus 1 mRNA export.

33 tion on the activities of the REF protein in mRNA export.

34 ing distinct stimulatory functions of CBC in mRNA export.

35 e noncoding nuclear RNA in the regulation of mRNA export.

36 Dbp5's activity can be modulated to regulate mRNA export.

37 slocation, thereby contributing to efficient mRNA export.

38 sing promotes ALYREF recruitment and histone mRNA export.

39 e REF1/Aly mRNA export adaptor to facilitate mRNA export.

40 e at a time, akin to a ratchet mechanism for mRNA export.

41 icating that RBM15 is required for efficient mRNA export.

42 ling a novel function of an F-box protein in mRNA export.

43 ght to remodel mRNA/protein complexes during mRNA export.

44 role for Ubp15 in coupling transcription to mRNA export.

45 are fundamental for the directional flow of mRNA export.

46 trosome integrity do not result from loss of mRNA export.

47 Mdm30 enhances Sub2's abundance and impairs mRNA export.

48 SDE5 is involved in basal plant defense and mRNA export.

49 unction, thus advancing our understanding of mRNA export.

50 partially due to the NSs dependent block in mRNA export.

51 activity in vitro and in vivo to facilitate mRNA export.

52 nucleus, leading to elevated eIF4E-dependent mRNA export.

53 R proteins underlies the regulation of their mRNA export activities and distinguishes pluripotent fro

54 ubnuclear residency, cell proliferation, and mRNA export activities through nuclear Akt phosphorylati

55 The REF/ALY mRNA export adaptor binds TAP/NXF1 via an arginine-rich

56 to identify the binding sites of ALYREF, an mRNA export adaptor in TREX, in human cells.

57 ICP27 also interacts with the mRNA export adaptor protein Aly/REF, which binds cellula

58 Ser2P CTD and recruits Iws1 and the REF1/Aly mRNA export adaptor to facilitate mRNA export.

59 e show that CBC promotes the targeting of an mRNA export adaptor, Yra1 (forming transcription export

60 Here we show that one CTA, Luzp4, is an mRNA export adaptor.

61 ate in mRNA export, such as nucleoporins and mRNA export adaptors, were mislocalized to SGs.

62 rs a Uap56 binding motif, conserved in other mRNA export adaptors.

63 Messenger RNA (mRNA) export adaptors play an important role in the tran

64 er previously described mutants with altered mRNA export affect cold signaling in a similar manner.

65 reas the deletion of residues 1-250 impaired mRNA export and also generated longer lag times when glu

66 lex couples nuclear pre-mRNA processing with mRNA export and contains multiple protein components, in

67 ter photobleaching to measure the binding of mRNA export and EJC core proteins in nuclear complexes.

68 pressed and activated GAL1 and also produced mRNA export and growth defects.

69 ne SUS1, which encodes a protein involved in mRNA export and histone H2B deubiquitination, contains t

70 How Rae1 functions in mRNA export and how M protein targets both Rae1 and Nup9

71 ent in binding NXF1-NXT1 does not block host mRNA export and is attenuated.

72 D-box ATPase Dbp5 (human DDX19) functions in mRNA export and is thought to remodel mRNPs at the nucle

73 e active PI3 kinase/AKT pathway can regulate mRNA export and promote the nuclear retention of some mR

74 Gle1(InsP6), Nup159 and Dbp5 collaborate in mRNA export and provide a general mechanism for DEAD-box

75 in-like protein 1 (Mlp1), which functions in mRNA export and quality control.

76 The last steps in mRNA export and remodeling are performed by the Nup82 co

77 ion activity of ALKBH5 significantly affects mRNA export and RNA metabolism as well as the assembly o

78 components functionally interact, affecting mRNA export and splicing as well as plant development.

79 components functionally interact, affecting mRNA export and splicing as well as plant development.

80 discovery of links between components of the mRNA export and splicing machineries and Sem1/Dss1, a co

81 ne region additively contribute to competent mRNA export and stress granule formation, both self-asso

82 We demonstrate that TgUAP56 is crucial for mRNA export and that functional interference leads to si

83 hough the molecular roles of Gle1 in nuclear mRNA export and translation have been documented, no ani

84 P(6)-binding mutants recapitulate all of the mRNA export and translation termination defects found in

85 Gle1 is the primary target of IP(6) for both mRNA export and translation termination in vivo.

86 ntiation of Dbp5 ATPase activity during both mRNA export and translation termination.

87 nal mode of Gle1 regulation to ensure proper mRNA export and translation.

88 equired for DEAD-box protein function during mRNA export and translation.

89 d the poly(A)-tail is required for efficient mRNA export and translation.

90 tifunctional regulator of DBPs with roles in mRNA export and translation.

91 ATPase proteins, with critical roles in both mRNA export and translation.

92 One factor investigated is an essential mRNA export and TREX complex component, Yra1.

93 credence for Nup functions in transcription, mRNA export, and genome organization.

94 of gene expression, including transcription, mRNA export, and post- transcriptional and translational

95 ssociates with the nuclear pores to regulate mRNA export, and regulates the circadian clock and flowe

96 ed that Aly/REF is not required for cellular mRNA export, and similar knockdown studies during HSV-1

97 A metabolism, including pre-mRNA processing, mRNA export, and translation.

98 ins function in nuclear pre-mRNA processing, mRNA export, and translation.

99 Tho1 is known to function in mRNA export, and we provide evidence that CIP29 likewise

100 ate that both the kinetics and efficiency of mRNA export are enhanced 6- to 10-fold (depending on the

101 studies indicated that 3' end processing and mRNA export are intertwined in gene regulation.

102 Key steps in mRNA export are the nuclear assembly of messenger ribonu

103 at Sem1 has a proteasome-independent role in mRNA export as a functional component of the Sac3-Thp1 c

104 nd HYPB/Setd2, in a megacomplex that affects mRNA export as well as the histone modification state of

105 This reversal of mRNA export block allowed expression of antiviral factor

106 The release of NS1-mediated mRNA export block by DHODH inhibition also occurred in t

107 down of Alyref and Chtop results in a potent mRNA export block.

108 und to mRNA in vivo and also causes a severe mRNA export block.

109 ndividual pores by applying various types of mRNA export blocks that stalled mRNPs at different stage

110 enzyme is required for efficient viral late mRNA export, but neither the relevant substrates nor the

111 ement for proper Gle1 oligomerization during mRNA export, but not for Gle1's roles in translation.

112 is shared by multiple mutants with aberrant mRNA export, but not in a mutant attenuated in nucleo-cy

113 virus 1 (HSV-1) protein ICP27 enables viral mRNA export by accessing the cellular mRNA export recept

114 sphosphate (IP(6)) play an essential role in mRNA export by activating the ATPase activity of the DEA

115 ta indicate that TREX provides a license for mRNA export by driving Nxf1 into a conformation capable

116 lasmic mRNA levels, suggesting that enhanced mRNA export by SRSF5 is required for the expression of p

117 Viral inhibition of cellular mRNA export can enhance viral access to the cellular tra

118 sphoinositide(s) regulates processes such as mRNA export, cell cycle progression, gene transcription,

119 for signal transduction pathways regulating mRNA export complex assembly, we used fluorescence recov

120 The conserved TREX mRNA export complex is known to contain UAP56, Aly, Tex1

121 earch of alternative pathways, we tested the mRNA export complex Mex67-Mtr2, for a role in tRNA nucle

122 It associates with the TREX mRNA export complex subunit Uap56 and harbours a Uap56 b

123 and MOS11 we detected interactions with the mRNA export complex TREX-2 and multiple spliceosomal com

124 bunit of the TRanscription-EXport-2 (TREX-2) mRNA export complex, promotes selective nuclear export o

125 result in efficient packaging into the TREX mRNA export complex, thereby supplanting the splicing re

126 , SAGA (Spt-Ada-Gcn5-Acetyltransferase), and mRNA export complex, TREX-2 (Transcription-export 2), an

127 bunit of the TRanscription-EXport-2 (TREX-2) mRNA export complex.

128 factor that binds both SAGA and the THO/TREX mRNA export complex.

129 codes a subunit of the highly conserved TREX mRNA-export complex, cause syndromic intellectual disabi

130 associate with transcription elongation and mRNA export complexes.

131 A polyadenylation signal, TREX mRNA export components, and the mRNA export receptor TAP

132 ZC3H3 by small interfering RNA results in an mRNA export defect in human cells as well.

133 ertheless, in tex1 mos11 double-mutants, the mRNA export defect was clearly enhanced relative to mos1

134 sed mRNA accumulation was observed, while no mRNA export defect was detected with tex1 cells.

135 se-causing Matrin 3 mutations led to nuclear mRNA export defects of both global mRNA and more specifi

136 ic96 binding sites in vivo yields growth and mRNA export defects, revealing their critical role in pr

137 ctive versions of Yra1 engendered growth and mRNA export defects.

138 1, mutant NS1 polypeptides unable to promote mRNA export did neither.

139 n enzymes, Mediator, and factors involved in mRNA export disrupts the physical interaction of chromos

140 e is implicated in crucial processes such as mRNA export, DNA editing, and phosphorus storage in plan

141 facilitate the coupling of transcription and mRNA export during gene expression.

142 te a pathway for the selective regulation of mRNA export during stress via retrotransposon activation

143 resent many roles in cells, participating in mRNA export, embryonic development, and apoptosis.

144 mRNA export factor 1 (Rae1) and nucleoporin 98 (Nup98) a

145 its 45% sequence similarity to human nuclear mRNA export factor 5.

146 ranslated region of RAD51 transcripts by the mRNA export factor ALY requires IPMK.

147 processes by reporting a novel role for the mRNA export factor Ddx19/Dbp5 in nuclear import of MKL1,

148 4 complex assembly positions the cytoplasmic mRNA export factor docking sites and messenger ribonucle

149 s of UAP56-interacting factor, which acts as mRNA export factor in mammalian cells.

150 y with other TREX complex components and the mRNA export factor Mex67.

151 gion (Sac3 approximately 1-100), which binds mRNA export factor Mex67:Mtr2; the M-region, in which Th

152 efective in the THO component TEX1 or in the mRNA export factor MOS11 (orthologue of human CIP29) are

153 In human cells, the mRNA export factor NXF1 resides in the nucleoplasm and a

154 Focusing on the major mRNA export factor NXF1, we found that initial mRNP bind

155 antly reduced following the silencing of the mRNA export factor Rae1, indicating that Rae1 is necessa

156 local homology to FG nucleoporins, the yeast mRNA export factor Sac3p, and the mammalian MCM3 acetylt

157 DEAD-box protein Rat8p/Dbp5p is an essential mRNA export factor that functions at the nuclear pore co

158 SDE5, encodes a putative mRNA export factor that is indispensable for transgene s

159 We investigated recruitment of the yeast mRNA export factor Yra1 to the transcription elongation

160 nteracts genetically and physically with the mRNA export factor Yra1.

161 otein, myosin-like protein 1 (Mlp1), and the mRNA export factor, Gfd1.

162 nucleus and cytoplasm in its role as a viral mRNA export factor.

163 translation initiation factor eIF3g and the mRNA-export factor DBP5.

164 n addition to the RNA helicase UAP56 and the mRNA export factors ALY2-4 and MOS11 we detected interac

165 is mediated by interactions between soluble mRNA export factors and distinct binding sites on the NP

166 s into the co-transcriptional recruitment of mRNA export factors and how this shapes the human transc

167 First, we show that mRNA export factors are highly enriched at the NPCs asso

168 Although essential mRNA export factors are known, the precise mechanism for

169 y which the MAP kinase Slt2 and Mlp1 control mRNA export factors during heat shock stress.

170 Here, we show that mRNA export factors generally facilitate usage of distal

171 e cytoplasmic side of the NPC, the conserved mRNA export factors Gle1 and inositol hexakisphosphate (

172 results indicate that UIEF1 and UIEF2 act as mRNA export factors in plants and that they cooperate wi

173 e nuclear envelope as well as the binding to mRNA export factors NXF1 and Aly/REF.

174 , an effect augmented by the presence of the mRNA export factors TAP, p15, or Rae1.

175 Rae1) and Nup98 are evolutionarily conserved mRNA export factors that are targeted by the vesicular s

176 UAP56, ALY/REF, and NXF1 are mRNA export factors that sequentially bind at the 5' end

177 rocessing and involved in the recruitment of mRNA export factors to nascent transcripts.

178 ncluding UL69's ability to interact with the mRNA export factors UAP56 and URH49 to facilitate the sh

179 uief and 4xaly (defective in the four ALY1-4 mRNA export factors) mutants produced the sextuple mutan

180 ly(A)-binding protein, Pab1p, and additional mRNA export factors.

181 Essential messenger RNA (mRNA) export factors execute critical steps to mediate d

182 results revealed not only a critical role of mRNA-export factors in transcriptional anti-silencing bu

183 (VSV-mp53) or enable [VSV-M(mut)-mp53] host mRNA export following infection of susceptible cells.

184 t block influenza A virus neuraminidase (NA) mRNA export from the nucleus and inhibit cytoplasmic NA

185 mRNA export from the nucleus depends on the ATPase activ

186 mRNA export from the nucleus is an essential step in the

187 mRNA export from the nucleus requires the RNA helicase U

188 the nuclear pore complex required for mature mRNA export from the nucleus to the cytoplasm, which mak

189 ly of the ribonucleoprotein complex prior to mRNA export from the nucleus.

190 ere, we show that UAP56 is required for bulk mRNA export from the nurse cell nuclei that supply most

191 Messenger RNA (mRNA) export from the nucleus is essential for eukaryoti

192 esults identify a mechanistic step in Gle1's mRNA export function at nuclear pore complexes and direc

193 cytoplasm, and significantly compromised its mRNA export function.

194 gnaling mediates both cell proliferation and mRNA export functions of Aly.

195 ally interacts with alleles of two essential mRNA export genes, MEX67 and YRA1.

196 on structure of the cytoplasmic nuclear pore-mRNA export holo-complex, challenging our textbook depic

197 response element (RRE) responsible for viral mRNA export, how it recruits multiple HIV Rev proteins t

198 svirus (KSHV), a nuclear DNA virus, inhibits mRNA export in a transcript-selective manner to control

199 interactions that are crucial for effective mRNA export in living cells, we examined mRNA export wit

200 ese et al. identify an alternate pathway for mRNA export in muscle cells where ribonucleoprotein comp

201 and Sub2), to the active genes and enhances mRNA export in Saccharomyces cerevisiae Likewise, recrui

202 to verify if these factors were involved in mRNA export in T. gondii.

203 sional, coarse-grained, agent-based model of mRNA export in the nanosecond regime to gain insight int

204 as we find evidence supporting Ran-dependent mRNA export in trypanosomes, similar to protein transpor

205 ctural elements are important for growth and mRNA export in vivo.

206 of Dbp5 at the NPC by Gle1 is essential for mRNA export in vivo; however, the mechanistic role of Db

207 data provide in vivo evidence for a model of mRNA export in which Nab2 is important for targeting mRN

208 r RNA binding are dominant-negative (DN) for mRNA export in yeast and human cells.

209 demonstrate that Mdm30p selectively controls mRNA export independently of mitochondrial fusion, revea

210 a transcription-dependent manner to promote mRNA export independently of splicing or mitochondrial f

211 ligase, these viral proteins stimulate viral mRNA export, inhibit cellular mRNA export, promote viral

212 ular target of NS1 and the mechanism of host mRNA export inhibition are not known.

213 rbance of the canonical molecular pathway of mRNA export is compatible with life but results in alter

214 mRNA export is highly conserved and ubiquitous.

215 Nuclear mRNA export is highly regulated to ensure accurate cellu

216 Our data support a model in which altered mRNA export is important for the manifestation of hos1 c

217 or investigating the means by which Dbp5 and mRNA export is modulated by regulatory factors.

218 However, such an impaired mRNA export is not dependent on mitochondrial fusion, as

219 On running the model, we observed that mRNA export is sensitive to the number and distribution

220 The TREX complex, which functions in mRNA export, is recruited to mRNA during splicing.

221 or proteasomal degradation in stimulation of mRNA export, it remains unknown whether such ubiquitin-p

222 e been best characterized for their roles in mRNA export, leaving their potential roles in splicing l

223 n at the posttranscriptional level including mRNA export/localization, stability, and translation.

224 NP as an integral component of the mammalian mRNA export machinery and suggest a model whereby GANP f

225 ription Export) complex, other components of mRNA export machinery are not well conserved in divergen

226 rising in light of the observations that the mRNA export machinery colocalizes with splicing factors

227 uclear pore complexes when components of the mRNA export machinery were mutated.

228 -2 as an integral component of the mammalian mRNA export machinery where it links transcription and n

229 AR-E, associates with components of the TREX mRNA export machinery, the Prp19 complex and U2AF2.

230 RNA by sequence-dependent recruitment of the mRNA export machinery.

231 mRNA processing is important for general mRNA export mediated by nuclear export factor 1 (Nxf1).

232 t on mitochondrial respiration/function, and mRNA export occurs in the absence of Fzo1, which is requ

233 The first is in the nucleus for mRNA export of most transcripts from the nucleus.

234 est an indirect role for factors involved in mRNA export or transcription.

235 the yeast Gle1 involved in the same poly(A) mRNA export pathway as Nup159, also result in seed abort

236 ngs implicate IPMK in a transcript-selective mRNA export pathway controlled by phosphoinositide turno

237 WNK1 (with no lysine [K] 1) in the mammalian mRNA export pathway even though it was previously establ

238 UAP56, and provides insight into a divergent mRNA export pathway in apicomplexans.

239 the Rev ARM and the successive Rev-dependent mRNA export pathway.

240 th TAP/NXF, providing a link to the cellular mRNA export pathway.

241 y binds to the mRNP at several stages of the mRNA export pathway.

242 defects in the classical protein import and mRNA export pathways in affected cells.

243 tion factor 4E (eIF4E) promotes translation, mRNA export, proliferation, and oncogenic transformation

244 timulate viral mRNA export, inhibit cellular mRNA export, promote viral gene expression, and direct t

245 In Saccharomyces cerevisiae, the mRNA export protein Yra1 (ALY/RNA export factor in metaz

246 the RNA pol II degradation factor Def1, the mRNA export protein Yra1 and the HECT E3 ligase Tom1.

247 LLY RESPONSIVE GENES4 [LOS4]) that regulates mRNA export, RCF1 does not play a role in mRNA export.

248 Here, we identify the target of NS1 as the mRNA export receptor complex, nuclear RNA export factor

249 Under normal conditions, we find that the mRNA export receptor Mex67 and Nab2 directly interact.

250 The conserved mRNA export receptor NXF1 (Mex67 in yeast) assembles wit

251 ly related to changes caused by depletion of mRNA export receptor NXF1 or the GANP subunit of the TRa

252 Luzp4 binds the principal mRNA export receptor Nxf1, enhances its RNA binding acti

253 hibition of the synthesis or activity of the mRNA export receptor Nxf1, which was observed to colocal

254 signal, TREX mRNA export components, and the mRNA export receptor TAP are required for accumulation o

255 viral mRNA export by accessing the cellular mRNA export receptor TAP/NXF, which guides mRNA through

256 ICP27 interacts with the mRNA export receptor TAP/NXF1 and binds RNA through an R

257 with TAP (Tip-associated protein; the major mRNA export receptor), and is a dynamic nuclear-cytoplas

258 Nxf1 is an mRNA export receptor, which binds processed mRNA and tra

259 lly paralleling the increasing complexity in mRNA export regulation and the evolution of new nuclear

260 ls, both proteins interact directly with the mRNA export-related RNA helicase UAP56 and the interacti

261 n which SG function requires hGle1A, whereas mRNA export requires hGle1B.

262 p5 molecules, the RNA helicase essential for mRNA export, revealed that Dbp5 most often approached th

263 Defects in the mRNA export scaffold protein GANP, encoded by the MCM3AP

264 onucleoprotein, Nab2, which is essential for mRNA export, specifically recognizes poly(A) RNA and bin

265 ologically relevant parameter regimes, a pre-mRNA export step can decrease steady-state variability a

266 ns as a mobile NPC component, which receives mRNA export substrates in the central channel of the NPC

267 ermore, nuclear proteins that participate in mRNA export, such as nucleoporins and mRNA export adapto

268 its Mediator-interacting surface to regulate mRNA export suggesting a mechanism for coupling transcri

269 bp5 ATPase activity is required for cellular mRNA export that is not met by the unstimulated enzyme,

270 plicing and polyadenylation to NXF1-mediated mRNA export, thereby controlling the cytoplasmic abundan

271 XF1-NXT1 to nucleoporins, thereby inhibiting mRNA export through the nuclear pore complex into the cy

272 facilitating SUS1 mRNA splicing to increase mRNA export through TREX-2, revealing distinct stimulato

273 ndicate that THO regulates pluripotency gene mRNA export to control ESC self-renewal and differentiat

274 d STAT5 dimer nuclear import and beta-casein mRNA export to cytoplasm affected the time delay between

275 ultimately shifts NMD that takes place after mRNA export to the cytoplasm to NMD that occurs before m

276 tein complex that is necessary for efficient mRNA export to the cytoplasm.

277 This in turn facilitates mRNA export to the cytoplasm.

278 regulated, including ATXN7L3B, which couples mRNA export to transcription activation by association w

279 om the chromatin template and messenger RNA (mRNA) export to the cytoplasm.

280 Thus, Ddx19 participates in mRNA export, translation and nuclear import of a key tra

281 s gene expression at multiple steps: nuclear mRNA export, translation initiation, and translation ter

282 NA helicases, with critical roles defined in mRNA export, translation initiation, translation termina

283 at the 5' cap of eukaryotic mRNA, regulating mRNA export, translation, and splicing.

284 RNA-dependent DEAD-box proteins required for mRNA export, translation, and stress responses.

285 e, recruitment of Npl3 (an hnRNP involved in mRNA export via formation of export-competent ribonuclea

286 ation of Sub2 in the absence of Mdm30 alters mRNA export via splicing defects of export factors and/o

287 adenylation factors, or Sus1 (that regulates mRNA export via transcription export 2 [TREX-2]).

288 ge and polyadenylation factors, and enhances mRNA export via TREX and RNP, respectively, in addition

289 NA and, consequently, Sus1 protein level and mRNA export via TREX-2.

290 mplex (EJC) recruitment to mRNA was reduced, mRNA export was obstructed, and cytoplasmic poly(A)+ RNA

291 A interference, the efficiency of viral late mRNA export was reduced to a corresponding degree.

292 Given the importance of Nab2 and Mlp1 to mRNA export, we have examined the Nab2/Mlp1 interaction

293 ed biophysical and biochemical parameters of mRNA export, we implemented a three-dimensional, coarse-

294 In the context of mRNA export, we propose that a given mRNA segment, after

295 4-7] in higher eukaryotes efficiently blocks mRNA export, whereas knockdown of REF only causes a mode

296 ic analysis to identify orthologs related to mRNA export, which show a remarkable low level of conser

297 teractions in yeast causes severe defects in mRNA export, while the severing of a single interaction

298 our data reconcile the broad role of ALY1 in mRNA export with the specific loss of RdDM through the a

299 GANP depletion inhibits mRNA export, with retention of mRNPs and NXF1 in punctat

300 ive mRNA export in living cells, we examined mRNA export within individual pores by applying various