ライフサイエンスコーパス: meiotic spindle

1 ension necessary to align chromosomes on the meiotic spindle.

2 ion of these proteins with p-PKC zeta at the meiotic spindle.

3 required for this loss of cyclin B from the meiotic spindle.

4 own as the spindle pole body or SPB) and the meiotic spindle.

5 GTP is polarized in the cortex overlying the meiotic spindle.

6 anes which envelop and invade the developing meiotic spindle.

7 ics drive the self-organization of a bipolar meiotic spindle.

8 X chromosomes to opposite poles on the first meiotic spindle.

9 romosome to opposite poles on the developing meiotic spindle.

10 s of PKC, results in rapid disruption of the meiotic spindle.

11 t facilitates their proper attachment to the meiotic spindle.

12 here it serves as the precursor to the first meiotic spindle.

13 disrupting organization of the MTOC-TMA and meiotic spindle.

14 TOC-TMA and subsequent assembly of the first meiotic spindle.

15 trosome precludes Golgi association with the meiotic spindle.

16 calreticulin and is notably absent from the meiotic spindle.

17 itioned with respect to their homolog on the meiotic spindle.

18 ocalized to the base of the MTOC-TMA and the meiotic spindle.

19 s that make up knobs to move poleward on the meiotic spindle.

20 oocytes degenerate and fail to form a second meiotic spindle.

21 esulting in sperm DNA within 2 microm of the meiotic spindle.

22 red for proper structure and function of the meiotic spindle.

23 ents the sperm DNA from interacting with the meiotic spindle.

24 ulin (colocalized with CaM kinase II) on the meiotic spindle.

25 ctly overlying the metaphase-arrested second meiotic spindle.

26 ted irregularities of the zona pellucida and meiotic spindle.

27 ve reduction in the mobility of knobs on the meiotic spindle.

28 a ring-shaped band of cortex adjacent to the meiotic spindle.

29 in the capture of the sperm contents by the meiotic spindle.

30 romosomes to microtubules of the mitotic and meiotic spindle.

31 ical F-actin to prevent interaction with the meiotic spindle.

32 constraining chromosomes in the acentrosomal meiotic spindle.

33 nd significantly larger than that of the pig meiotic spindle.

34 the origin of asymmetric positioning of the meiotic spindle.

35 12 pN to move a chromosome on the mitotic or meiotic spindle.

36 attachment of unpartnered chromosomes to the meiotic spindle.

37 ogues so they orientate appropriately on the meiotic spindle.

38 a sperm aster that could capture the oocyte meiotic spindle.

39 lizing the bipolar shape of the acentrosomal meiotic spindle.

40 crotubule flux in Xenopus laevis egg extract meiotic spindles.

41 nd Caenorhabditis elegans mitotic and female meiotic spindles.

42 the construction and dynamics of mitotic and meiotic spindles.

43 means for increasing microtubule density in meiotic spindles.

44 ndensing chromosomes and associates with the meiotic spindles.

45 length, an idea that has not been tested in meiotic spindles.

46 the MTOC-TMA and subsequent assembly of the meiotic spindles.

47 h as the dynamic asters found in mitotic and meiotic spindles.

48 ated from preexisting ones within mitotic or meiotic spindles.

49 lel/antiparallel microtubule organization in meiotic spindles.

50 mediated assembly of extremely long bipolar meiotic spindles.

51 nit, MEI-1, cause specific defects in female meiotic spindles.

52 rotubule organization and dynamics in oocyte meiotic spindles.

53 Increased meiotic spindle abnormalities and aneuploidy in oocytes

54 mosomal misalignment on the metaphase plate, meiotic spindle abnormalities, or mitochondrial dysfunct

55 In the absence of centrosomes, meiotic spindles achieve bipolarity by a combination of

56 requires specific positioning of the second meiotic spindle, achieved by dynein-driven transport, an

57 utinus, centrioles organize the poles of the meiotic spindle and are inherited by both the polar body

58 Commonalities in meiotic spindle and chromosome alignment defects under t

59 e more general APC(Fzy), both locally on the meiotic spindle and globally in the egg cytoplasm, to ta

60 d maintaining bipolarity of the acentrosomal meiotic spindle and in promoting the contacts that the c

61 We find that spindlin associates with the meiotic spindle and is modified by phosphorylation in a

62 that global cortical contraction forces the meiotic spindle and overlying membrane out through the c

63 y-pGV oocyte is similar to that of the mouse meiotic spindle and significantly larger than that of th

64 aM kinase II) is tightly associated with the meiotic spindle and that 5 min after egg activation ther

65 We show that Mtrm is enriched along the meiotic spindle and that loss of mtrm results in misloca

66 omplex to keep chromosomes within the oocyte meiotic spindle and that this function likely involves t

67 product functions in the organization of the meiotic spindle and the formation of long microtubules.

68 imately 2.5-fold between the cortex over the meiotic spindle and the opposite cortex, suggesting that

69 nsition showed PKC-delta associated with the meiotic spindle and then with the chromosomes at MII.

70 lent chromosomes could align normally on the meiotic spindle and whether metaphase spermatocytes woul

71 d oocytes supported the formation of de novo meiotic spindles and, after fertilization with sperm, me

72 toskeleton and required for migration of the meiotic spindle, and a second affecting the spindle micr

73 Xiwi is associated with microtubules and the meiotic spindle, and is localized to the germ plasm--a c

74 Cdc23 localized on the meiotic spindle, and microinjection of Cdc23 siRNA cause

75 of the cortex and proper orientation of the meiotic spindle, and thus we hypothesized that cortical

76 tive mitotic translation occurs on X. laevis meiotic spindles, and a subset of microtubule-bound mRNA

77 The ultra-structure of meiotic spindles, and how changes to this structure corr

78 Mitotic and meiotic spindles are assemblies of microtubules (MTs) th

79 In animals, female meiotic spindles are attached to the egg cortex in a per

80 many animals, including vertebrates, oocyte meiotic spindles are bipolar but assemble in the absence

81 Meiotic spindles are disorganized, pronuclear migration

82 Combining these approaches, we find that meiotic spindles are dynamic arrays of short microtubule

83 During female meiosis, meiotic spindles are positioned at the oocyte cortex to

84 Meiotic spindles are positioned perpendicular to the ooc

85 etochore microtubules (non-kMTs), vertebrate meiotic spindles are predominantly comprised of non-kMTs

86 We propose that without non-kMTs, metaphase meiotic spindles are similar to mammalian mitotic spindl

87 Anastral meiotic spindles are thought to be organized differently

88 Previous studies have proposed that these meiotic spindles arise from RanGTP-mediated MT nucleatio

89 the partners, allowing them to attach to the meiotic spindle as a unit, such that they migrate away f

90 PKCzeta is tightly associated with the meiotic spindle as determined by detergent extraction an

91 ts indicate that bipolar but abnormal oocyte meiotic spindles assemble in aspm-1(-) embryos, whereas

92 In most animals, female meiotic spindles assemble in the absence of centrosomes;

93 e used to deplete non-kMTs in the vertebrate meiotic spindle assembled in Xenopus egg extracts.

94 lation quantitatively reproduces features of meiotic spindles assembled in Xenopus egg extracts.

95 In contrast to somatic cells, the first meiotic spindle assembles in the absence of centriole-co

96 ill address recent work on the mechanisms of meiotic spindle assembly and chromosome alignment/segreg

97 We report that PP2A is essential for meiotic spindle assembly and chromosome dynamics during

98 Accurate meiotic spindle assembly and chromosome segregation - es

99 e they mediate nuclear anchoring, as well as meiotic spindle assembly and rotation, two processes req

100 s to show that PLK-1 plays distinct roles in meiotic spindle assembly and/or stability, chromosome al

101 nes previously shown to contribute to oocyte meiotic spindle assembly are the calponin homology domai

102 s involved in both the timing of location of meiotic spindle assembly as well as the coordination of

103 atanin MT-severing activity is essential for meiotic spindle assembly but is toxic for the mitotic sp

104 ependent microtubule reorganization promotes meiotic spindle assembly by facilitating the search and

105 ta suggest a model whereby the LISD promotes meiotic spindle assembly by serving as a reservoir that

106 Interfering with early steps of meiotic spindle assembly can lead to erroneous chromosom

107 Meiotic spindle assembly commences when microtubules gai

108 ly examine microtubule reorganization during meiotic spindle assembly in living Drosophila oocytes.

109 We developed a model of early meiotic spindle assembly in mouse oocytes, including key

110 otor protein in Drosophila that functions in meiotic spindle assembly in oocytes and spindle pole mai

111 on along the pole-to-pole axis, we simulated meiotic spindle assembly in two dimensions using dynamic

112 at overcome arrest exhibit severe defects in meiotic spindle assembly, chromosome segregation, and cy

113 ssed the chromatin-based mechanism of female meiotic spindle assembly, it is less clear how signaling

114 Based on a 2D model of meiotic spindle assembly, we predicted that higher local

115 icrotubule number during the early stages of meiotic spindle assembly.

116 that this regulation is required for bipolar meiotic spindle assembly.

117 p80, both of which are essential for female meiotic spindle assembly.

118 itis elegans, is required for bipolar oocyte meiotic spindle assembly.

119 re during the acentrosomal process of oocyte meiotic spindle assembly.

120 ng complex/cyclosome (APC/C) activation, and meiotic spindle assembly.

121 to MTs, compromising its function in female meiotic-spindle assembly.

122 ere found to be strongly associated with the meiotic spindle at all stages of meiosis II; however, no

123 We investigated the mechanism by which meiotic spindles become bipolar and the correlation betw

124 tes, fertilized by wild-type sperm, set up a meiotic spindle but do not progress to anaphase I.

125 on of aPKCzeta also causes elongation of the meiotic spindle but still permits spindle migration and

126 lls are absent highlighting a control of the meiotic spindle by the OGT-O-GlcNAcase duo.

127 , some mechanism must prevent capture of the meiotic spindle by the sperm aster.

128 We used an assay system in which hundreds of meiotic spindles can be observed forming around chromati

129 or the proper functioning of the mitotic and meiotic spindle checkpoints (MSCs), which monitor the in

130 est; extrusion rather than extraction of the meiotic spindle-chromosome complex (SCC); nuclear transf

131 , and is concentrated at the point where the meiotic spindle contacts the F-actin-rich cortex.

132 Meiotic spindles contain more microtubules than their mi

133 Our data suggest that non-kMTs in meiotic spindles contribute to normal kMT dynamics, stab

134 Mispositioning of the meiotic spindle, defects in polar body extrusion and chr

135 ry of female meiosis and the polarity of the meiotic spindle dictate that the partner with the greate

136 In contrast, we find that the female meiotic spindle does not scale as closely to egg size, a

137 his structure, which we term the liquid-like meiotic spindle domain (LISD), permeates the spindle pol

138 a wave that initiates in the vicinity of the meiotic spindle during anaphase I.

139 ng meiotic maturation and concentrate on the meiotic spindle during metaphases I and II.

140 d on the vegetal side (the side opposite the meiotic spindle) during maturation.

141 scopy identified that Kif2a localized to the meiotic spindle, especially concentrated at the spindle

142 P identified that Cep55 was localized to the meiotic spindle, especially to the spindle poles at meta

143 In the absence of DAZL synthesis, the meiotic spindle fails to form due to disorganization of

144 abilize microtubules, which is essential for meiotic spindle formation and accurate chromosome segreg

145 These studies identify meiotic spindle formation and programmed degradation of

146 ritical process for ensuring the fidelity of meiotic spindle formation and proper chromosome segregat

147 These oocytes are protected from abnormal meiotic spindle formation through the recruitment of O-G

148 nucleolus chromatin morphology, and abnormal meiotic spindle formation was observed following oocyte

149 peroxisome biogenesis, endosome sorting and meiotic spindle formation, but functions for the SF7 AAA

150 Meiotic spindle formation, however, is not affected in t

151 icrotubule-severing activity and its role in meiotic spindle formation, we analyzed the MEI-1(A338S)

152 ompensate for the lack of centrosomes during meiotic spindle formation.

153 t loss of telomere-LINC contacts compromises meiotic spindle formation.

154 r DYNLRB2 are separately used in mitotic and meiotic spindle formations, respectively, and that both

155 ed mechanisms underlying differences between meiotic spindles formed in egg extracts of two frog spec

156 gamma-Tubulin was stably associated with the meiotic spindle from prometaphase-1 through to anaphase-

157 f the spindle to BAPTA and EGTA-suggest that meiotic spindle function in frog oocytes requires highly

158 esin-like protein, SUB, that is required for meiotic spindle function.

159 s I and meiosis II, and dissociates from the meiotic spindle in anaphase II.

160 shwork that facilitates translocation of the meiotic spindle in asymmetric division of mouse oocytes.

161 te closure of the NE opening surrounding the meiotic spindle in C. elegans oocytes.

162 the proper organization of the acentrosomal meiotic spindle in Drosophila melanogaster oocytes.

163 ts in plants, the asymmetric position of the meiotic spindle in mammalian embryos, and the developmen

164 We have studied the formation of the first meiotic spindle in murine oocytes from mice homozygous f

165 nstrated the variable position of the second meiotic spindle in relation to the first polar body; con

166 tomographic reconstructions of spermatocyte meiotic spindles in Caenorhabditis elegans, we find the

167 Female meiotic spindles in many organisms form in the absence o

168 Most meiotic spindles in mei-38 oocytes are bipolar but poorl

169 Human oocytes assembled a meiotic spindle independently of either centrosomes or o

170 In contrast, Caenorhabditis elegans female meiotic spindles initially shorten in the pole-to-pole a

171 lead to circular mitotic figures and loss of meiotic spindle integrity.

172 that CSNK-1 prevents expulsion of the entire meiotic spindle into a polar body by negatively regulati

173 During female meiosis in animals, the meiotic spindle is attached to the egg cortex by one pol

174 In mouse oocytes, the first meiotic spindle is formed through the action of multiple

175 aberrant chromosome behavior a stable first meiotic spindle is not formed, the spindle poles continu

176 In animals, the female meiotic spindle is positioned at the egg cortex in a per

177 owing fertilization the small, barrel-shaped meiotic spindle is replaced by a large zygotic spindle t

178 The meiotic spindle is thought to provide a scaffold that me

179 -crossover partners become bioriented on the meiotic spindle is unknown.

180 for the assembly and maintenance of bipolar meiotic spindles, is not needed to maintain spindle bipo

181 ought to play a major role in organizing the meiotic spindle, it remains unclear how a stable bipolar

182 ave also been reported for C. elegans female meiotic spindles, it is not clear whether they are repre

183 The female meiotic spindle lacks a centrosome or microtubule-organi

184 embly of this bipolar spindle, and while the meiotic spindle lacks traditional centrosomes, some cent

185 hip between microtubule disassembly rate and meiotic spindle length.

186 We have characterized male meiotic spindle lengths in wild-type and the ask1-1 muta

187 Although mitotic and meiotic spindles maintain a steady-state length during m

188 the hermaphrodite gonad and is localized to meiotic spindle microtubules in the newly fertilized emb

189 nes directly adjacent to NE holes containing meiotic spindle microtubules.

190 in formation and maintenance of mitotic and meiotic spindles, move in opposite directions along micr

191 In Caenorhabditis elegans oocytes, meiotic spindle movement toward the cortex before anapha

192 cent protein (GFP) to monitor changes in the meiotic spindle of live oocytes after activation in vitr

193 The meiotic spindles of animal eggs move to extremely asymme

194 ulates the production of microtubules in the meiotic spindles of Caenorhabditis elegans oocytes.

195 sted oocytes are relatively stable, however, meiotic spindles of in vitro-activated oocytes are highl

196 Meiotic spindles of metaphase-arrested oocytes are relat

197 alpha 85E does not disrupt meiotic spindle or cytoplasmic microtubules but causes d

198 gation-promoting chromosome alignment on the meiotic spindle or some physical interaction between hom

199 ds to a significant (P < 0.05) disruption of meiotic spindle organization and chromosome alignment du

200 MTOC-associated proteins and plays a role in meiotic spindle organization in mammalian oocytes.

201 nts show that HSET activity is essential for meiotic spindle organization in murine oocytes and taxol

202 role of protein kinase C delta (PKCdelta) on meiotic spindle organization was evaluated in mouse oocy

203 es, such as chromosome coalescence, aberrant meiotic spindle organization, and the expression of a me

204 motor proteins are essential for mitotic and meiotic spindle organization, chromosome segregation, or

205 nce that kinesin-5 contributes to C. elegans meiotic spindle organization.

206 Oocyte meiotic spindles orient with one pole juxtaposed to the

207 If incorporated into the meiotic spindle, paternal chromosomes could be expelled

208 microtubule severing and ASPM-1 both promote meiotic spindle pole assembly in C. elegans oocytes, whe

209 t2p is required for linking telomeres to the meiotic spindle pole body (SPB) but not for attachment o

210 ion factor EAP30, Dot2, negatively regulates meiotic spindle pole body (SPB, the yeast equivalent of

211 hTOG exclusively at spindle poles to support meiotic spindle pole stabilization during male meiosis,

212 n, PKCdelta expression was restricted to the meiotic spindle poles and a few specific cytoplasmic foc

213 TAC-1 is initially enriched at the meiotic spindle poles and is later recruited to the sper

214 n with microtubules and with LIN-5-ASPM-1 at meiotic spindle poles and that the APC promotes spindle

215 The acentrosomal meiotic spindle poles do not have centrioles and are not

216 g enzyme that is concentrated at mitotic and meiotic spindle poles in animals.

217 hat phosphorylated GM130 associates not with meiotic spindle poles, but with ER clusters in the matur

218 P-190, and CP-60 are not concentrated at the meiotic spindle poles.

219 ity, is required for assembly of acentriolar meiotic spindle poles.

220 Although meiotic spindle positioning in oocytes has been investig

221 try in oocytes by participating in eccentric meiotic spindle positioning, sperm incorporation cone dy

222 tcc-1 and unc-116 causes similar defects in meiotic spindle positioning, supporting the concept of T

223 Although normal bipolar meiotic spindles predominate during the first week, oocy

224 ulted in chromosomes being expelled from the meiotic spindle prior to anaphase onset, a more severe p

225 terestingly, in embryos lacking an organized meiotic spindle, produced either by nocodazole treatment

226 is defined by the position of the persistent meiotic spindle rather than by the position of the sperm

227 ules, we have characterized the movements of meiotic spindles relative to the cell cortex.

228 ocytes lack centrioles and, therefore, mouse meiotic spindle relies on the organization of numerous a

229 Assembly of Caenorhabditis elegans female meiotic spindles requires both MEI-1 and MEI-2 subunits

230 s to identify molecular factors important in meiotic spindle scaling.

231 Wild-type C. elegans meiotic spindle shortening proceeds through an early kat

232 ind that egg extracts from H. boettgeri form meiotic spindles similar in size to X. tropicalis but th

233 In frogs, the meiotic spindle size is positively correlated with the e

234 us frog species possess a variety of egg and meiotic spindle sizes, and differences in activities or

235 Importantly, the disruption of meiotic spindle stability was associated with decreased

236 MTOCs, which functions in the regulation of meiotic spindle stability.

237 Defects in meiotic spindle structure can lead to chromosome segrega

238 Defects in meiotic spindle structure contribute to chromosome segre

239 sted oocytes were characterized by disrupted meiotic spindle structure, reduced microtubule density a

240 es have been found to be enriched at the MII meiotic spindle, such as Protein Kinase C (PKC), and are

241 cal granule-free domain (CGFD) overlying the meiotic spindle that is in close proximity to the cortex

242 ays, whereas weaker mutants assemble bipolar meiotic spindles that are longer than wild type.

243 d from the mutants revealed microtubules and meiotic spindles that were close to normal even in the a

244 spindle and flip toward the egg side of the meiotic spindle, thereby achieving preferential segregat

245 and orient the homologous chromosomes on the meiotic spindle to ensure proper segregation at meiosis

246 tive mutants causes both halves of the first meiotic spindle to extend symmetrically toward opposing

247 microtubule severing in translocation of the meiotic spindle to the cortex.

248 asymmetry results from the anchoring of the meiotic spindle to the oocyte cortex and subsequent cort

249 n range from the assembly and positioning of meiotic spindles to the prevention of cytoplasmic stream

250 t be consistent with direct transport of the meiotic spindle toward the cortex by kinesin-1.

251 identified a motor complex that may move the meiotic spindle toward the cortex in Caenorhabditis eleg

252 lymerization to initiate the movement of the meiotic spindle toward the cortex, an essential step in

253 t step in asymmetric positioning of anastral meiotic spindles, translocation to the cortex.

254 Most female meiotic spindles undergo striking morphological changes

255 their later interdependent attachment to the meiotic spindle using tension-sensing biorientation mach

256 atisfaction was achieved because the bipolar meiotic spindle was assembled more quickly in the absenc

257 osin-10 (Myo10) is important for assembly of meiotic spindles, we assessed the role of this unconvent

258 When two meiotic spindles were brought close enough together, the

259 irst polar bodies in culture; however, their meiotic spindles were often positioned parallel, instead

260 ays a subtle role in formation of the female meiotic spindle which is acentriolar, but is essential f

261 y dependent on asymmetric positioning of the meiotic spindle, which is established through migration

262 n in eukaryotes depends upon the mitotic and meiotic spindles, which assemble at the time of cell div

263 n at the opposite end of the embryo from the meiotic spindle while yolk granules are transported thro

264 exposure and suggest that the oocyte and its meiotic spindle will provide a sensitive assay system fo

265 Human oocytes are prone to assembling meiotic spindles with unstable poles, which can favor an

266 onsense mutation in MEI-1 caused assembly of meiotic spindles without any poles as assayed by localiz