ライフサイエンスコーパス: metabolic labeling

1 of Skp1, Skp1 was not destabilized based on metabolic labeling.

2 d secretion of VWF and this was confirmed by metabolic labeling.

3 ecipitation, cell surface biotinylation, and metabolic labeling.

4 mbardment MS, detailed NMR spectrometry, and metabolic labeling.

5 IR or sham radiation followed by brief (32)P metabolic labeling.

6 n ceruloplasmin were examined by pulse-chase metabolic labeling.

7 itro with purified proteins and in vivo with metabolic labeling.

8 n global protein synthesis, as determined by metabolic labeling.

9 tools for analyzing mass spectral data from metabolic labeling.

10 corporated into yeast and mouse proteins via metabolic labeling.

11 on as verified by both mass spectrometry and metabolic labeling.

12 Because metabolic labeling allows internal control throughout sa

13 Metabolic labeling also indicated that a significant fra

14 Metabolic labeling analysis demonstrated that, to compen

15 We describe the combined use of 15N-metabolic labeling and a cysteine-reactive biotin affini

16 f glycosylated tissues in live mice by using metabolic labeling and a gadolinium-based bioorthogonal

17 on in primary CLL cells, measured using bulk metabolic labeling and a novel flow cytometry assay to q

18 understand the molecular pathogenesis using metabolic labeling and assays of proinsulin export and i

19 ntroduce unnatural functional groups through metabolic labeling and chemoenzymatic tagging; identific

20 Ac status of proteins using a combination of metabolic labeling and click chemistry-based mass taggin

21 In this study, we performed metabolic labeling and immunofluorescence staining of ne

22 Metabolic labeling and immunofluorescence studies of COS

23 Using a metabolic labeling and immunoprecipitation approach, we

24 ive for point mutations in the elastin gene, metabolic labeling and immunoprecipitation experiments w

25 Metabolic labeling and immunoprecipitation studies revea

26 Metabolic labeling and immunoprecipitation studies with

27 approximately 8 h in human) as determined by metabolic labeling and immunoprecipitation with anti-GCa

28 ive rates of leptin biosynthesis measured by metabolic labeling and immunoprecipitation.

29 Metabolic labeling and in vitro enzyme assays confirmed

30 of glycosaminoglycan (GAG) were examined via metabolic labeling and liquid chromatography.

31 m the endoplasmic reticulum (ER) as shown by metabolic labeling and live-cell imaging.

32 Metabolic labeling and mass spectrometric analyses sugge

33 importance, this technique does not require metabolic labeling and may be used as a pharmacodynamic

34 We developed an assay that uses metabolic labeling and MS to associate chromatin accessi

35 o determining the structure of melanin using metabolic labeling and NMR spectroscopy.

36 escribe a genetic AND gate for cell-targeted metabolic labeling and proteomic analysis in complex cel

37 Using metabolic labeling and pulse-chase analysis of HIV-1 Gag

38 Metabolic labeling and reporter gene assays demonstrated

39 f newly synthesized ferritin was analyzed by metabolic labeling and SDS-PAGE electrophoresis.

40 Metabolic labeling and top-down mass spectrometry reveal

41 treatment were first identified using (15)N metabolic labeling and untargeted mass spectrometry with

42 Analysis by immunoblotting, metabolic labeling, and mass spectrometry demonstrated t

43 -dimensional gel electrophoresis techniques, metabolic labeling, and stable isotope labeling methods

44 raightforward application of methods such as metabolic labeling, and the sequenced genome laid the fo

45 ent methods, quantitative mass spectrometry, metabolic labeling, and Western blotting.

46 Here we describe a metabolic labeling approach based on incorporation of no

47 of ribose and base methylations, and a novel metabolic labeling approach is presented to allow identi

48 Metabolic labeling approaches identify the general prote

49 Transcription inhibition experiments and metabolic labeling assays argue that REF/Aly does not af

50 In contrast, metabolic labeling assays showed that SAHA decreased inc

51 Metabolic labeling assays showed that targeting these th

52 Metabolic labeling assays, FADS3 overexpression and knoc

53 We combine metabolic labeling, bioorthogonal chemistry, and super-r

54 ER exit of ENaC was assayed after metabolic labeling by following the appearance of cleave

55 These results demonstrate that metabolic labeling can be used to provide additional con

56 Metabolic labeling, cell surface biotinylation, immobili

57 Here, combining metabolic labeling, click-chemistry and enzymatic reacti

58 ned by enzyme-linked immunosorbent assay and metabolic labeling, COL1A1 steady-state mRNA levels, and

59 Our work demonstrates the power of metabolic labeling combined with stable isotopic dilutio

60 Pulse-chase metabolic labeling confirmed that inhibiting calpains in

61 Using metabolic labeling, confocal immunofluorescence microsco

62 Using metabolic labeling coupled with cell surface biotinylati

63 Metabolic labeling coupled with sulfoamino acid analysis

64 K2 in a dose- and time-dependent manner, and metabolic labeling demonstrated that geldanamycin rapidl

65 Metabolic labeling demonstrates that these cysteines in

66 RNA metabolic labeling demonstrates that, in FMRP-deficient

67 Ingenious metabolic labeling enables facile imaging of glycostruct

68 A cell metabolic labeling experiment can be completed in approx

69 We carried out metabolic labeling experiments and studies of mice with

70 However, analysis of data from metabolic labeling experiments can be complicated becaus

71 Metabolic labeling experiments confirmed a reduced half-

72 Furthermore, metabolic labeling experiments demonstrated carbon from

73 Metabolic labeling experiments demonstrated hypoxia-medi

74 Radiotracer and metabolic labeling experiments demonstrated specific cel

75 RNA blot hybridization, immunostaining, and metabolic labeling experiments demonstrated that SVAS ce

76 Metabolic labeling experiments demonstrated that the pre

77 Metabolic labeling experiments in primary hepatocytes fr

78 However, metabolic labeling experiments indicate that Syk is indu

79 be quite complex, in particular with in vivo metabolic labeling experiments producing fractional atom

80 Metabolic labeling experiments provided direct evidence

81 Metabolic labeling experiments revealed that Cia protein

82 Metabolic labeling experiments revealed that IFN had lit

83 Moreover, well-controlled cotransfection and metabolic labeling experiments revealed that VHL missens

84 Confocal microscopy and metabolic labeling experiments show that the total pool

85 Metabolic labeling experiments showed that class I is re

86 these more stringent regulatory properties, metabolic labeling experiments showed that coenzyme A (C

87 Finally metabolic labeling experiments showed that the cardiac r

88 Metabolic labeling experiments showed that the proteasom

89 This is in agreement with in vivo metabolic labeling experiments showing that fucose is no

90 lity was further examined in immunoblots and metabolic labeling experiments using two time points.

91 Through metabolic labeling experiments we demonstrate that the r

92 Furthermore, metabolic labeling experiments with (13)C-glucose showed

93 acylation of expressed HA as demonstrated by metabolic labeling experiments with [(3)H]palmitate.

94 Metabolic labeling experiments with [(35)S]methionine in

95 35S-Metabolic labeling experiments with domain-specific surf

96 Metabolic labeling experiments with transgenic parasites

97 Envelope is useful for planning or designing metabolic labeling experiments, by visualizing hypotheti

98 Alleviating the need for GALE-KO cells in metabolic labeling experiments, GalNAzMe is a precision

99 By reverse genetics and metabolic labeling experiments, we demonstrate that two

100 e rates of HDV RNA synthesis, as measured by metabolic labeling experiments, were identical at 4 and

101 DOC secreted the Cia proteins, as judged by metabolic labeling experiments.

102 hages synthesize P-selectin, as indicated by metabolic labeling experiments.

103 Metabolic labeling followed by immunoprecipitation revea

104 Metabolic labeling followed by immunoprecipitation verif

105 icular proteins by in vivo [(35)S]methionine metabolic labeling followed by preparation of highly pur

106 munofluorescence microscopy, immunoblotting, metabolic labeling, immunoprecipitation, and carbohydrat

107 Metabolic labeling, immunoprecipitation, and SDS-polyacr

108 K44A, as detected by three distinct methods: metabolic labeling, immunoprecipitation/Western blotting

109 We now find, by metabolic labeling-immunoprecipitation experiments, that

110 Here, using metabolic labeling, immunoprecipitations, various bioche

111 Metabolic labeling in combination with mutagenesis indic

112 Current work utilized metabolic labeling in cultured cells expressing wild typ

113 Metabolic labeling in vivo demonstrated that E7 proteins

114 many of the possible issues associated with metabolic labeling, including low incorporation of sugar

115 Metabolic labeling indicated that the synthesis of these

116 se-chase experiments using [(35)S]methionine metabolic labeling indicated that the turnover rate of d

117 detached from the membrane, and results from metabolic labeling indicated that these cells accumulate

118 Metabolic labeling, ion exchange and size exclusion chro

119 many available isotopic labeling strategies, metabolic labeling is attractive for the excellent inter

120 Recently, we reported a novel metabolic labeling method to introduce the diazirine pho

121 By applying MS in conjunction with a metabolic labeling method, we defined a PP1/2A-sensitive

122 Current metabolic labeling methods rely on exogenous pyrimidine

123 ine these technologies with (13)C- and (15)N-metabolic labeling, multiple derivatization and ionizati

124 Isobaric tagging and metabolic labeling, namely, tandem mass tagging (TMT) an

125 Metabolic labeling of a mutant PC12 cell line, A123.7, e

126 However, metabolic labeling of A34 transgenic mice with (75)Se re

127 can also be used to determine intracellular metabolic labeling of amino acids from glucose carbons.

128 In this study, metabolic labeling of bacteria with fatty acid chemical

129 seria meningitidis serogroup B to facilitate metabolic labeling of bacterial endotoxin and compared i

130 c cell membrane was used in conjunction with metabolic labeling of bacterial proteins to identify chl

131 bundance and half-life were determined after metabolic labeling of CCS-/- fibroblasts transfected wit

132 Metabolic labeling of cells expressing the siRNA to GRWD

133 sly identify the relevant peptide from TSR1, metabolic labeling of cells expressing TSR1 and the cyst

134 Metabolic labeling of cells using heavy amino acids is m

135 Reciprocal (14)N/(15)N metabolic labeling of cells was used to measure the rela

136 Kinetic metabolic labeling of cells with [3H]-leucine indicated

137 The metabolic labeling of cells with an alkynyl derivative o

138 Metabolic labeling of cellular proteins with [35S]methio

139 Metabolic labeling of cellular RNA is a powerful approac

140 s examined by immunoblot analysis and (64)Cu metabolic labeling of Chinese hamster ovary cells transf

141 Metabolic labeling of CHO-K1 cells or Lec35.1 cells demo

142 to the biological system of interest (i.e., metabolic labeling of clinical samples, most animals, or

143 Metabolic labeling of de novo lipogenesis (DNL) using (2

144 through in vivo [(14)C]acetate and [(3)H]2O metabolic labeling of developing seeds surprisingly reve

145 Metabolic labeling of DGA1 deletion strains with triglyc

146 is and turnover were examined following 64Cu metabolic labeling of fibroblasts derived from CCS+/+ an

147 ar abnormal Tf IEF patterns were analyzed by metabolic labeling of fibroblasts with inverted question

148 Metabolic labeling of glycans with a bioorthogonal chemi

149 Metabolic labeling of glycans with synthetic sugar analo

150 Metabolic labeling of hCTPS2 with [(32)P]H(3)PO(4) demon

151 on, fluorescence-activated cell sorting, and metabolic labeling of HCV-specific proteins.

152 Metabolic labeling of hexaacylated endotoxin (LOS) from

153 Metabolic labeling of hippocampal slices shows increased

154 ferential [(14)C]acetate and [(14)C]malonate metabolic labeling of hydroxylase-expressing seeds indic

155 Steady-state metabolic labeling of loa1Delta revealed a significant r

156 Metabolic labeling of M. bovis BCG showed that at least

157 rial activity of macrophages was assessed by metabolic labeling of M. tuberculosis with [3H]uracil.

158 ficiencies were quantitated by intracellular metabolic labeling of monocistronic mRNAs and the dicist

159 In addition, metabolic labeling of MR cells with (75)Se revealed a lo

160 lation of smaller molecules was supported by metabolic labeling of mtDNA with [3H]thymidine during re

161 Both metabolic labeling of nascent transcripts and an unbiase

162 e analysis of transcriptional initiation via metabolic labeling of nascent transcripts in patient-der

163 In this method, metabolic labeling of newly synthesized proteins with AH

164 e analysis of transcriptional initiation via metabolic labeling of newly synthesized transcripts in l

165 Metabolic labeling of newly synthesized viral RNA follow

166 The approach was validated by metabolic labeling of nuclear pore protein p62, which is

167 ic strategies and ribozymes, and finish with metabolic labeling of nucleic acids.

168 thod utilizes an O-GlcNAc azide analogue for metabolic labeling of O-GlcNAc-modified proteins, which

169 Metabolic labeling of one organism containing an orphan

170 rate of PPARgamma protein but decreased the metabolic labeling of PPARgamma protein using [(35)S]met

171 Metabolic labeling of primary hepatocytes indicated that

172 Using metabolic labeling of primary islets and a cultured beta

173 Coupling APEX2 labeling of lysosomes and metabolic labeling of protein, we identify that individu

174 Cell-selective metabolic labeling of proteins with noncanonical amino a

175 The long-term metabolic labeling of rats with a diet enriched in 15N d

176 Metabolic labeling of recombinant interferon-beta and Gl

177 Here we combine metabolic labeling of RNA at high temporal resolution wi

178 we developed a chemical-genetic strategy for metabolic labeling of RNA.

179 hich contains an alkyne and a diazirine, for metabolic labeling of S-palmitoylated proteins and photo

180 Metabolic labeling of S. aureus confirms that CoA levels

181 Metabolic labeling of sialic acids in fibroblasts confir

182 ion was found on serum proteins, and reduced metabolic labeling of sialic acids was found in fibrobla

183 ids modified with a sydnone reporter for the metabolic labeling of sialoconjugates.

184 Recent development in metabolic labeling of small biomolecules allows the stud

185 We have used metabolic labeling of staphylococcal cultures with [(32)

186 Metabolic labeling of the fibroblast cultures was used t

187 In IsoTaG, metabolic labeling of the glycoproteome is combined with

188 When the pool is filled with nascent ATP, metabolic labeling of the Na(+)/K(+) or Ca(2+) pump phos

189 Metabolic labeling of the protein in vivo indicates that

190 However, selective metabolic labeling of the target tissues in vivo remains

191 Metabolic labeling of THP-1 cells did not show release o

192 pectrometry with clinically accepted in vivo metabolic labeling of tissue with deuterium to generate

193 Metabolic labeling of transfected Chinese hamster ovary

194 geranylgeranylation of Rab24, determined by metabolic labeling or detergent partitioning assays, is

195 Following metabolic labeling or SEEL, tagged glycoproteins were en

196 Metabolic labeling or separation by isoelectric focusing

197 essment of purities of labeled compounds and metabolic labeling patterns requires careful analysis of

198 Using metabolic labeling, phosphoamino acid analysis, and muta

199 Using a [32P]orthophosphate metabolic labeling procedure to study HDV RNA replicatio

200 antification of stable isotope tracers after metabolic labeling provides a snapshot of the dynamic st

201 mical labeling quantification in addition to metabolic labeling quantification.

202 Metabolic labeling reaffirmed that metformin promoted wi

203 Indeed, metabolic labeling revealed an increased usage of ethano

204 Immunoblot analysis and pulse-chase metabolic labeling revealed that hephaestin is synthesiz

205 Metabolic labeling revealed that only wild type galectin

206 Metabolic labeling reveals a notable deficit in the rate

207 Pulse-chase metabolic labeling reveals that the half-life of TIM-1 i

208 icing rates genome-wide in Drosophila, using metabolic labeling/RNA sequencing and new mathematical m

209 Here, we combine RNA metabolic labeling, rRNA-depleted RNA-seq, and DRiLL, a

210 Metabolic labeling showed incorporation of [(3)H]myristi

211 The results of in vivo metabolic labeling showed that Rosi markedly reduced de

212 nnosylation sites), using mass spectrometry, metabolic labeling, site-directed mutagenesis, and expre

213 oration in cell or tissue culture ((1)N/(1)N metabolic labeling, stable isotope labeling by amino aci

214 evaluate postgrowth Cys-labeling and 14N/15N metabolic labeling strategies for determination of relat

215 litated using chemical tags such as ICAT and metabolic labeling strategies with stable isotopes.

216 tude of dynamic range which is comparable to metabolic labeling strategies.

217 ecently developed neutron encoding (NeuCode) metabolic labeling strategy and parallel reaction monito

218 We have used a bioorthogonal metabolic labeling strategy to detect cell surface glyca

219 Here, we use a metabolic labeling strategy to directly measure nucleoso

220 In the 14N/15N metabolic labeling strategy, we achieve 98% 15N incorpor

221 Metabolic labeling studies and phosphopeptide mapping re

222 Using metabolic labeling studies as well as site-directed muta

223 Metabolic labeling studies demonstrate that unlike wild

224 Pulse-chase metabolic labeling studies demonstrated that the SPP hom

225 Using steady-state kinetics and in vivo metabolic labeling studies in modified yeast strains, we

226 Metabolic labeling studies in vivo demonstrate agonist-s

227 Metabolic labeling studies indicate that TfR2 protein le

228 Metabolic labeling studies indicated that this reflected

229 Metabolic labeling studies of Fibrillin-1 in human SSc d

230 Metabolic labeling studies of T. cruzi suggested that st

231 Moreover, results of metabolic labeling studies showed that downregulation of

232 Metabolic labeling studies showed that overexpression of

233 These findings were further supported by metabolic labeling studies that showed [1-(14)C]acetate

234 Metabolic labeling studies using [14C]20:4,n6 (at 100 mi

235 Metabolic labeling studies were conducted in freshly iso

236 Metabolic labeling studies with [(13)C]glucose showed th

237 sing phospho-specific Western blot analysis, metabolic labeling studies, and whole-cell signaling exp

238 Here we show, with metabolic labeling studies, that lopinavir leads to the

239 This progerin was farnesylated, as judged by metabolic labeling studies.

240 arbonitrile (PCN)-treated rats using in vivo metabolic-labeling studies with [35S]cysteine/methionine

241 Further, metabolic labeling suggests that silencing occurs before

242 Here, we use a recently developed metabolic labeling technique, NeuCode (neutron encoding)

243 ly confirmed using a 35S-methionine/cysteine metabolic labeling technique, whereas APP mRNA level rem

244 ation of cellular RNAs with polysomes and by metabolic labeling, that PDK-1-/- embryonic stem (ES) ce

245 By using standards generated from in vitro metabolic labeling, the relative quantitation of four pe

246 Here, we used approach-to-steady-state metabolic labeling to assess these rates for 176 miRNAs

247 al-abundance or (15)N-labeled algae, we used metabolic labeling to compare protein levels in colonic

248 by this hormone in vivo, we used (14)N/(15)N metabolic labeling to perform a quantitative untargeted

249 alance during activation of T cells, we used metabolic labeling to quantify the contributions of RNA

250 arval zebrafish or paired with cell-specific metabolic labeling to visualize circuits underlying memo

251 rotein-labeling methods, such as chemical or metabolic labeling, to realize the same benefits.

252 reased Gln uptake and ammonia secretion, and metabolic labeling using (13)C-Gln revealed that Hace1 l

253 Metabolic labeling using sugar analogs compatible with c

254 Phosphorylation of DAT assessed by (32)PO(4) metabolic labeling was increased up to 2-fold by in vitr

255 antennae family mRNAs, in vivo 4-thiouracil metabolic labeling was used to distinguish synthesis and

256 surface cross-linking, FRET, and pulse-chase metabolic labeling, we demonstrate that deleting the cyt

257 Using immunoprecipitation coupled with metabolic labeling, we demonstrate that the alpha4 subun

258 sition of endoglycosidase H resistance after metabolic labeling, we found no evidence of ER retention

259 of gene expression and in vivo and in vitro metabolic labeling, we found that TbPSS2 (i) is necessar

260 By immunofluorescence and pulse-chase metabolic labeling, we found that the soluble constructs

261 By metabolic labeling, we here identify phosphatidylinosito

262 By using metabolic labeling, we measured Abeta42 and Abeta40 prod

263 bimolecular fluorescence complementation and metabolic labeling, we show that GABA(B) receptors assoc

264 this study, using in situ hybridization and metabolic labeling, we show that the mRNAs encoding euka

265 he set of tools available for cell-selective metabolic labeling, we sought a MetRS variant capable of

266 This was demonstrated by metabolic labeling, Western blotting, flow cytometry, an

267 The SILIS was produced through metabolic labeling where (1)(5)N was uniformly introduce

268 ted Raman scattering microscopy coupled with metabolic labeling with (13)C-phenylalanine is used to v

269 Metabolic labeling with (32)P(i) indicated that the aero

270 In vivo phosphorylation was studied by metabolic labeling with (32)P-orthophosphate.

271 tent was determined by IRMA and synthesis by metabolic labeling with (35)S-cysteine in organ cultures

272 Metabolic labeling with (64)Cu demonstrated that the red

273 GU in Aeromonas salmonicida was confirmed by metabolic labeling with (75) Se or mass spectrometry.

274 This computational screen and subsequent metabolic labeling with (75)Se and characterization of s

275 that TLT-1 is a palmitoylated protein using metabolic labeling with [(3)H]palmitate and identified t

276 Metabolic labeling with [(3)H]palmitate determined that

277 lls, and the H1 proteins were analyzed after metabolic labeling with [(3)H]palmitate.

278 nine lens epithelial cells was determined by metabolic labeling with [(35)S]-methionine.

279 Metabolic labeling with [(35)S]cysteine was used to char

280 ation with membrane-impermeable reagents and metabolic labeling with [(35)S]methionine followed by im

281 Using metabolic labeling with [(35)S]methionine to measure sub

282 Using metabolic labeling with [(35)S]methionine, the half-life

283 ynthesis in these cultures was determined by metabolic labeling with [(35)S]methionine.

284 ucceeded in detecting HDV RNA replication by metabolic labeling with [32P]orthophosphate in vivo and

285 ractions, the endotoxins were purified after metabolic labeling with [3H]- or [14C]acetate.

286 Metabolic labeling with alkyne-oleic acid (100 mum for 1

287 Metabolic labeling with alkyne-palmitic acid (100 mum fo

288 itative proteomics studies of yeast that use metabolic labeling with amino acids rely on auxotrophic

289 ribe the dynamics of mass isotopomers during metabolic labeling with an atom-based stable isotope.

290 Metabolic labeling with atom-based heavy isotopes, follo

291 Glycans can be imaged by metabolic labeling with azidosugars followed by chemical

292 an emerging strategy, glycans are imaged by metabolic labeling with chemical reporters and subsequen

293 Metabolic labeling with cytochrome oxidase (CO) and elec

294 Here we describe the use of metabolic labeling with deuterium ((2)H) from (2)H(2)O a

295 Metabolic labeling with GalNAz followed by Staudinger li

296 azidoacetylmannosamine (ManNAz), showed that metabolic labeling with GalNAz resulted in the greatest

297 Bax on two-dimensional gels and confirmed by metabolic labeling with inorganic [(32)P]phosphate in He

298 O-linked glycoproteins in living animals by metabolic labeling with N-azidoacetylgalactosamine (GalN

299 Metabolic labeling with S(35)-methionine was used to det

300 to the cross-linker (primarily deuterium) or metabolic labeling with SILAC.