ライフサイエンスコーパス: methyltetrahydrofolate

1 cal amounts of FA or the reduced folate, L-5-methyltetrahydrofolate.

2 revent excessive conversion of methylene- to methyltetrahydrofolate.

3 ction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate.

4 inding of folic acid and by the uptake of N5-methyltetrahydrofolate.

5 hionine and flavodoxin but unreactive toward methyltetrahydrofolate.

6 The cofactor is then remethylated by methyltetrahydrofolate.

7 nzyme activity, leading to lower levels of 5-methyltetrahydrofolate.

8 for the (6S) and (6R) diastereoisomers of N5-methyltetrahydrofolate.

9 the receptor in the observed transport of N5-methyltetrahydrofolate.

10 talyze methylation of free cob(I)alamin with methyltetrahydrofolate.

11 ate than the reactions with homocysteine and methyltetrahydrofolate.

12 e conversion of methylenetetrahydrofolate to methyltetrahydrofolate.

13 methionine using a methyl group derived from methyltetrahydrofolate.

14 ated to form l-methionine by betaine or N(5)-methyltetrahydrofolate.

15 ystal structure of rat GNMT complexed with 5-methyltetrahydrofolate.

16 is inhibited by a specific form of folate, 5-methyltetrahydrofolate.

17 hionine by using a methyl group derived from methyltetrahydrofolate.

18 ation from MtvC to tetrahydrofolate, forming methyltetrahydrofolate.

19 late; and (3) determination of total liver 5-methyltetrahydrofolate.

20 method involves (1) determination of liver 5-methyltetrahydrofolate; (2) chemical reduction of liver

21 The pH dependence of the uptake of [(3)H]-methyltetrahydrofolate ([(3)H]-MTF) was assayed in Mulle

22 for the circulating folate coenzyme, (6S)-5-methyltetrahydrofolate (5-CH3H4folate), and its opposite

23 y trapping folate cofactors in the form of 5-methyltetrahydrofolate (5-methylTHF) and subsequent inhi

24 ductase (MTHFR) catalyzes the synthesis of 5-methyltetrahydrofolate (5-methylTHF), the methyl donor f

25 otal folate and individual folate vitamer [5-methyltetrahydrofolate (5-methylTHF), unmetabolized FA (

26 We have investigated the ability of 5-methyltetrahydrofolate (5-MTHF) and tetrahydrobiopterin

27 We identified FA and 5-methyltetrahydrofolate (5-mTHF) by retention time and ch

28 Plasma MTX, leucovorin, and 5-methyltetrahydrofolate (5-mTHF) concentrations were meas

29 to release folic acid (FA) and endogenous 5-methyltetrahydrofolate (5-MTHF) from infant milk formula

30 ith normal serum folate levels and low CSF 5-methyltetrahydrofolate (5-MTHF) levels.

31 The circulating form of folic acid, 5-methyltetrahydrofolate (5-MTHF), may have beneficial eff

32 as to analyze the long-term effects of FO, 5-methyltetrahydrofolate (5-MTHF), or FO+5-MTHF prenatal s

33 We demonstrate that 5-methyltetrahydrofolate (5-MTHF, the predominant folate i

34 licitation of different forms of folates - 5-methyltetrahydrofolate, 5-formyltetrahydrofolate and 10-

35 ations of folic acid, 10-formylfolic acid, 5-methyltetrahydrofolate, 5-formyltetrahydrofolate and tet

36 rmination and high enzymatic production of 5-methyltetrahydrofolate, 5-formyltetrahydrofolate and tet

37 ween maternal folate status as measured by 5-methyltetrahydrofolate (5MeTHF), 5-formyltetrahydrofolat

38 to circulating unmetabolized folic acid or 5-methyltetrahydrofolate (5MeTHF).

39 Data on serum 5-methyltetrahydrofolate (5MTHF) and folic acid (FA) conce

40 rent forms of folate, folic acid (FA), and 5-methyltetrahydrofolate (5mTHF), at concentrations of 5 m

41 ssociated with elevated serine but reduced 5-methyltetrahydrofolate (5MTHF).

42 old) greater relative affinities for (6S)-N5-methyltetrahydrofolate, (6S)-N5-formyltetrahydrofolate a

43 thylenetetrahydrofolate reductase (MTHFR) (5-methyltetrahydrofolate:(acceptor) oxidoreductase, EC 1.7

44 f the folate derivatives demonstrated that 5-methyltetrahydrofolate accounts for 30% of total cellula

45 be involved in the binding and activation of methyltetrahydrofolate, amino acids 650-896 are responsi

46 lded predominantly polyglutamates of [(3)H]5-methyltetrahydrofolate and [(3)H]5-formyltetrahydrofolat

47 cysteine is dependent on the production of 5-methyltetrahydrofolate and adequate vitamin B-12 for the

48 derstanding the biochemical balance in using methyltetrahydrofolate and betaine as methyl donors as w

49 mics and kinetics of methyl transfer between methyltetrahydrofolate and cob(I)alamin or cob(I)inamide

50 the cobalamin is alternatively methylated by methyltetrahydrofolate and demethylated by homocysteine

51 synthesizing serine, and (3) it sequesters 5-methyltetrahydrofolate and inhibits SAM synthesis.

52 lNC females had higher BW and lower plasma 5-methyltetrahydrofolate and methionine consistent with lo

53 was specific for such reduced folates as N5-methyltetrahydrofolate and N5-formyltetrahydrofolate.

54 yzes the methylation of free cob(I)alamin by methyltetrahydrofolate and the methylation of homocystei

55 sources that lead to the production of N(5)-methyltetrahydrofolate and the remethylation of l-homocy

56 Serum 5-methyltetrahydrofolate and vitamin B-12 concentrations w

57 to 10-fold, 3) did not change affinity for 5-methyltetrahydrofolate, and 4) except for E45R decreased

58 ion of free folic acid, tetrahydrofolate, 5'-methyltetrahydrofolate, and 5'-formyltetrahydrofolate in

59 The LOQ and LOD for tetrahydrofolate, 5'-methyltetrahydrofolate, and 5'-formyltetrahydrofolate we

60 th three different substrates: homocysteine, methyltetrahydrofolate, and S-adenosyl-l-methionine (Ado

61 etetrahydrofolate (stabilized at pH 10) to 5-methyltetrahydrofolate; and (3) determination of total l

62 a cobalamin-dependent reaction that utilizes methyltetrahydrofolate as a methyl group donor.

63 alyzes the methylation of homocysteine using methyltetrahydrofolate as the methyl donor.

64 We evaluated whether a high-dose L-5-methyltetrahydrofolate-based regimen provided improved t

65 to high-dose folic acid, high-dose oral L-5-methyltetrahydrofolate-based supplementation does not af

66 nterconversion of all folate vitamers into 5-methyltetrahydrofolate before 14 h of germination and hi

67 ocysteine binding region (residues 2-353), a methyltetrahydrofolate binding region (residues 354-649)

68 ent manner, and (2) cSHMT, a high affinity 5-methyltetrahydrofolate-binding protein, sequesters this

69 pathway by transferring a methyl group from methyltetrahydrofolate bound to a methyltransferase to t

70 chloroplasts and lowered the proportion of 5-methyltetrahydrofolate but did not discernibly affect gr

71 ion, the enzyme reacts with homocysteine and methyltetrahydrofolate but is unreactive toward adenosyl

72 rsion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate by MTHFR.

73 alyzes the transfer of methyl groups between methyltetrahydrofolate (CH(3)-H(4)folate) and homocystei

74 The methyltetrahydrofolate (CH(3)-H(4)folate) corrinoid-iron

75 N5-Methyltetrahydrofolate (CH(3)-H(4)folate) donates a meth

76 lyzes the transfer of the N5-methyl group of methyltetrahydrofolate (CH(3)-H(4)folate) to the sulfur

77 lenetetrahydrofolate (CH(2)-H(4)folate) to 5-methyltetrahydrofolate (CH(3)-H(4)folate) using flavin a

78 lenetetrahydrofolate (CH(2)-H(4)folate) to 5-methyltetrahydrofolate (CH(3)-H(4)folate) using flavin a

79 lenetetrahydrofolate (CH(2)-H(4)folate) to 5-methyltetrahydrofolate (CH(3)-H(4)folate) using NADH as

80 thesis of methionine from homocysteine and 5-methyltetrahydrofolate (CH(3)-H(4)folate) using the uniq

81 enetetrahydrofolate (CH(2)-H(4)folate) to N5-methyltetrahydrofolate (CH(3)-H(4)folate), committing a

82 the folate (Fol) domain for remethylation by methyltetrahydrofolate (CH(3)-H(4)folate).

83 lenetetrahydrofolate (CH(2)-H(4)folate) to 5-methyltetrahydrofolate (CH(3)-H(4)folate).

84 nthesis using the triglutamate derivative of methyltetrahydrofolate (CH(3)-H(4)PteGlu(3)) as methyl d

85 ntinuous transmethylation of homocysteine by methyltetrahydrofolate (CH(3)THF) to form methionine.

86 The cobalt center is methylated by methyltetrahydrofolate (CH3-H4folate) to form a methylco

87 yzes transfer of the N5-methyl group of (6S)-methyltetrahydrofolate (CH3-H4folate) to the cob(I)amide

88 es transfer of the N5-methyl group from (6S)-methyltetrahydrofolate (CH3-H4folate) to the cobalt cent

89 ein (C/Fe-SP) by the N5 methyl group of (6S)-methyltetrahydrofolate (CH3-H4folate).

90 cantly correlated with cerebrospinal fluid 5-methyltetrahydrofolate concentrations, which were below

91 The methyltetrahydrofolate:corrinoid/iron-sulfur protein met

92 The methyltetrahydrofolate:corrinoid/iron-sulfur protein met

93 The methyltetrahydrofolate:corrinoid/iron-sulfur protein met

94 the cell surface and increases the rate of 5-methyltetrahydrofolate delivery.

95 cob(I)alamin cofactor: methyl transfer from methyltetrahydrofolate during primary turnover and methy

96 smegmatis utilization of monoglutamylated 5-methyltetrahydrofolate exogenously added to the medium.

97 tetrahydrofolate reductase (MTHFR) generates methyltetrahydrofolate for methylation reactions.

98 ethylation with [(2)H(2)]methyl groups (as 5-methyltetrahydrofolate) formed only from cytosolic proce

99 This protein accepts a methyl group from methyltetrahydrofolate forming Me-Co(3+)CFeSP that then

100 biological circulating folate derivatives, 5-methyltetrahydrofolate, from folate receptors.

101 de SAH hydrolase, methionyl-tRNA synthase, 5-methyltetrahydrofolate:Hcy methyltransferase, S-adenosyl

102 Transport of N5-methyltetrahydrofolate in human retinal pigment epitheli

103 ve been found necessary for potocytosis of 5-methyltetrahydrofolate in MA104.

104 an (+/- SEM) rate of appearance of [(13)C5]5-methyltetrahydrofolate in plasma was 0.33 +/- 0.09 (capl

105 tep in the pathway, but also tightly binds 5-methyltetrahydrofolate in the cytoplasm, a required cofa

106 rculating concentrations of folic acid and 5-methyltetrahydrofolate in the Framingham Offspring Cohor

107 steady-state reactions with homocysteine and methyltetrahydrofolate in the presence of phenol red, we

108 mount (17 mg/day) of the reduced folate, L-5-methyltetrahydrofolate, in addition to 50.0 mg/day of vi

109 Folic acid and its active metabolite, 5-methyltetrahydrofolate, increase endothelium-dependent v

110 olate and its derivatives methotrexate and 5-methyltetrahydrofolate induced H(+)-coupled inward curre

111 ethotrexate, 5-formyltetrahydrofolate, and 5-methyltetrahydrofolate initial rates and net uptake in c

112 anion exchanger that mediates delivery of 5-methyltetrahydrofolate into mammalian cells.

113 acteristics of the uptake of radiolabeled N5-methyltetrahydrofolate into the cells were investigated.

114 Transport of N5-methyltetrahydrofolate into these cells occurred by a si

115 5-Methyltetrahydrofolate is a major methyl donor in the re

116 In contrast, when 5-methyltetrahydrofolate is depleted by alcohol consumptio

117 ant route by which the major blood folate, 5-methyltetrahydrofolate, is transported into mammalian ce

118 emperature to study the stability of 1mM l-5-methyltetrahydrofolate (l-5-MTHF) in combination with ep

119 A cyclic voltammetry study of 1mM l-5-methyltetrahydrofolate (l-5-MTHF) was performed in pH 5.

120 stability of free and microencapsulated L-5-methyltetrahydrofolate (L-5-MTHF) with free folic acid (

121 Human cDNAs for methionine synthase (5-methyltetrahydrofolate:L-homocysteine S-transmethylase;

122 HF was inhibited by the structural analogs 5-methyltetrahydrofolate, methotrexate and folic acid (K(i

123 Although 5-methyltetrahydrofolate (methylTHF) was higher in materna

124 hylating dUMP to dTMP in DNA synthesis, to 5-methyltetrahydrofolate (methylTHF), the primary methyl d

125 throughout treatment and concentrations of 5-methyltetrahydrofolate (MTF), methionine (MET), SAM, and

126 essed by determining the uptake of [3H]-N(5)-methyltetrahydrofolate (MTF).

127 or an equimolar amount (17 mg/d) of oral L-5-methyltetrahydrofolate (MTHF group).

128 lly pure 6R- and 6S-3'-aza-2'-(18)F-fluoro-5-methyltetrahydrofolate (MTHF) (6R-(18)F-1 and 6S-(18)F-1

129 The 6R and 6S isomers of (18)F-aza-5-methyltetrahydrofolate (MTHF) were assessed regarding th

130 The main vacuolar folate was 5-methyltetrahydrofolate, of which 51% was polyglutamylate

131 e folate was vacuolar and was again mainly 5-methyltetrahydrofolate, of which 76% was polyglutamylate

132 oss of activity is slowed in the presence of methyltetrahydrofolate or adenosylmethionine.

133 t of pemetrexed; influx of folic acid, (6S)5-methyltetrahydrofolate, or (6S)5-formyltetrahydrofolate

134 of the folate increase was contributed by 5-methyltetrahydrofolate polyglutamates and 5,10-methenylt

135 5-Methyltetrahydrofolate polyglutamates were the only fola

136 5-Methyltetrahydrofolate, SAM, and SAM/S-adenosylhomocyste

137 Especially in faba bean, 5-methyltetrahydrofolate showed surprisingly good stabilit

138 rmal proportions of one-carbon forms, with 5-methyltetrahydrofolate the most abundant, but were less

139 ficiency via the accumulation of folate as 5-methyltetrahydrofolate (the "methyl trap").

140 These studies, done with N(5)-methyltetrahydrofolate (the predominant folate derivativ

141 ahydrofolate reductase (MTHFR) synthesizes 5-methyltetrahydrofolate, the major carbon donor in remeth

142 e conversion of methylenetetrahydrofolate to methyltetrahydrofolate, the major methyl donor for the c

143 he reduction of methylenetetrahydrofolate to methyltetrahydrofolate, the methyl donor for the convers

144 MTHFR null mutants (mthfr(-)) lacked 5-methyltetrahydrofolate, the most abundant intracellular

145 ction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, the predominant circulatory form

146 red for purine and thymidine syntheses, to 5-methyltetrahydrofolate, the primary circulatory form of

147 onine and cob(I)alamin enzyme, and then from methyltetrahydrofolate to cob(I)alamin enzyme, generatin

148 the ability to catalyze methyl transfer from methyltetrahydrofolate to exogenous cob(I)alamin, but ha

149 teine but do not affect methyl transfer from methyltetrahydrofolate to exogenous cob(I)alamin, consis

150 ar protein that is alternately methylated by methyltetrahydrofolate to form methylcobalamin and demet

151 methylcobalamin to homocysteine but not from methyltetrahydrofolate to free cob(I)alamin.

152 et reaction, transfer of a methyl group from methyltetrahydrofolate to homocysteine (Hcy) to form met

153 se catalyzes a methyl transfer reaction from methyltetrahydrofolate to homocysteine to form methionin

154 atalyzes the transfer of a methyl group from methyltetrahydrofolate to homocysteine to form methionin

155 atalyzes the transfer of a methyl group from methyltetrahydrofolate to homocysteine to form tetrahydr

156 in which a methyl group is transferred from methyltetrahydrofolate to homocysteine to generate tetra

157 atalyzes the transfer of a methyl group from methyltetrahydrofolate to homocysteine to give tetrahydr

158 atalyzes the transfer of a methyl group from methyltetrahydrofolate to homocysteine to produce methio

159 that catalyzes a methyl group transfer from methyltetrahydrofolate to homocysteine via a methylcob(I

160 atalyzes the transfer of a methyl group from methyltetrahydrofolate to homocysteine via the enzyme-bo

161 atalyzes the transfer of a methyl group from methyltetrahydrofolate to homocysteine, forming tetrahyd

162 atalyzes the transfer of a methyl group from methyltetrahydrofolate to homocysteine, generating tetra

163 the protein to catalyze methyl transfer from methyltetrahydrofolate to homocysteine.

164 atalyzes the transfer of a methyl group from methyltetrahydrofolate to homocysteine.

165 ntermediate carrier of the methyl group from methyltetrahydrofolate to homocysteine.

166 MetF also catalyzes the oxidation of methyltetrahydrofolate to methylenetetrahydrofolate in t

167 this, leaf tissues metabolized [methyl-(14)C]methyltetrahydrofolate to serine, sugars, and starch.

168 ric methods are based on the conversion of 5-methyltetrahydrofolate to tetrahydrofolate by methionine

169 e enzyme then catalyzes methyl transfer from methyltetrahydrofolate to the cob(I)alamin cofactor, for

170 In contrast, binding methyltetrahydrofolate to the enzyme does not result in

171 group that is in transit from one substrate (methyltetrahydrofolate) to another (homocysteine).

172 itamin D(3)], folate vitamers (folic acid, 5-methyltetrahydrofolate, total folates), and fatty acids

173 cells do not contain detectable levels of 5-methyltetrahydrofolate under the same culture conditions

174 In addition, the characteristics of N5-methyltetrahydrofolate uptake in these cells were compar

175 ction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, used to methylate homocysteine i

176 he reduction of methylenetetrahydrofolate to methyltetrahydrofolate, using NADH as the reductant.

177 hydrofolate reductase (MTHFR), synthesizes 5-methyltetrahydrofolate, utilized in homocysteine remethy

178 tains the regions that bind homocysteine and methyltetrahydrofolate utilizes exogenously supplied cob

179 ynthesis of methionine from homocysteine and methyltetrahydrofolate via two methyl transfer reactions

180 The folate receptor-mediated transport of 5-methyltetrahydrofolate was almost completely blocked in

181 receptor-mediated transport of [3H]-(6S)-N5-methyltetrahydrofolate was much more efficient in L1210A

182 This reaction is regulated by 5-methyltetrahydrofolate, which inhibits the enzyme cataly

183 sole enzyme responsible for generation of 5-methyltetrahydrofolate, which is required for methionine