ライフサイエンスコーパス: micrococcal nuclease

1 tects approximately 11 bp of linker DNA from micrococcal nuclease.

2 p of DNA is protected against digestion with micrococcal nuclease.

3 bited by a specific competitive inhibitor of micrococcal nuclease.

4 DNA fragments resulting from digestions with micrococcal nuclease.

5 the sensitivity of chromatin to digestion by micrococcal nuclease.

6 al DNA ladders sharper than those created by micrococcal nuclease.

7 d treatment of lysates with Ca(2+)-dependent micrococcal nuclease.

8 s even after digestion of the chromatin with micrococcal nuclease.

9 component is sensitive to cycloheximide and micrococcal nuclease.

10 iation to treatment with ethidium bromide or micrococcal nuclease.

11 decreased rate of digestion of chromatin by micrococcal nuclease.

12 ated by treatment with proteinase K, but not micrococcal nuclease.

13 leosomal DNA to Dam methyltransferase and to micrococcal nuclease.

14 the primary chromatin structure is probed by micrococcal nuclease.

15 c mRNA length) were found to be resistant to micrococcal nuclease (69%) or to remain suspended in ass

16 nization but were found to be susceptible to micrococcal nuclease (85%) or to sediment to a pellet in

17 Pretreatment of the U1 snRNP with micrococcal nuclease abolished the assembly of galectin-

18 vage activity is sensitive to treatment with micrococcal nuclease, also consistent with an activity a

19 Linker DNA was freely accessible to micrococcal nuclease, although the oligomers remained pa

20 High-resolution micrococcal nuclease analyses revealed that a positioned

21 Micrococcal nuclease analysis revealed that the heteroch

22 sults demonstrate increased DNA laddering by micrococcal nuclease and an increased amount of DNA inte

23 units are equally accessible to DNase I and micrococcal nuclease and contain similar levels of histo

24 n vitro and in vivo assays of sensitivity to micrococcal nuclease and dam methyltransferase, respecti

25 tivity of wild-type nuclei to digestion with micrococcal nuclease and deoxyribonuclease I, indicating

26 ecreased accessibility of their chromatin to micrococcal nuclease and DNase I digestion and increased

27 s of chromatin structure by accessibility to micrococcal nuclease and DNase I digestion demonstrated

28 core region of the insulator as revealed by micrococcal nuclease and DNase I digestion studies.

29 T118-I) are more accessible to digestion by micrococcal nuclease and do not constrain DNA in a preci

30 n mitoribosome footprints are generated with micrococcal nuclease and mitoribosomes are separated fro

31 s with defined ends, bulk NCPs prepared with micrococcal nuclease and molecular modelling to reassess

32 s no hypersensitivity to either DNase I or a micrococcal nuclease and no translational positioning of

33 complex, as supported by its sensitivity to micrococcal nuclease and proteinase K.

34 e of ribosomal chromatin was investigated by micrococcal nuclease and psoralen photocrosslinking.

35 undaries were determined by assays combining micrococcal nuclease and restriction endonuclease digest

36 region for several days, as demonstrated by micrococcal nuclease and restriction enzyme accessibilit

37 tuted mononucleosomes using exonuclease III, micrococcal nuclease and restriction enzymes demonstrate

38 Micrococcal nuclease and short-recognition-sequence blun

39 erformance liquid chromatography analysis of micrococcal nuclease and spleen phosphodiesterase-digest

40 In micrococcal nuclease and supercoiling assays, addition o

41 eabilization and digestion of chromatin with micrococcal nuclease and then compared tumor necrosis fa

42 digestion by DNAse, restriction enzymes, and micrococcal nuclease, and an increased affinity for GAL4

43 DNase I, micrococcal nuclease, and exonuclease III footprinting s

44 By combining DNase I, micrococcal nuclease, and specific restriction endonucle

45 atin in the spt6 mutant is hypersensitive to micrococcal nuclease, and this hypersensitivity is suppr

46 e flexibility and strongly blocked access of micrococcal nuclease as contour lengths shortened, consi

47 ain reaction, site-directed mutagenesis, and micrococcal nuclease assay to determine the role of S-ni

48 Gene expression profiling, micrococcal nuclease assay, and chromatin immunoprecipit

49 and increased mononucleosome generation in a micrococcal nuclease assay.

50 ccessibility, as indicated by results of the micrococcal nuclease assay.

51 Chromatin immunoprecipitation and micrococcal nuclease assays were performed to determine

52 om human metaphase chromosomes digested with micrococcal nuclease associate spontaneously forming mul

53 organization at subnucleosome resolution by micrococcal nuclease-based chromosome conformation captu

54 staining with ULI-NChIP-seq (ultra-low-input micrococcal nuclease-based native ChIP-seq) shows that E

55 we report single-cell Micro-C (scMicro-C), a micrococcal nuclease-based three-dimensional (3D) genome

56 The RTC becomes permeable to micrococcal nuclease but not to antibodies.

57 ent types of nucleosome remodeling events in micrococcal nuclease ChIP-seq (chromatin immunoprecipita

58 osome leading to an asymmetric protection to micrococcal nuclease cleavage of linker DNA relative to

59 itional perturbation is marked by changes in micrococcal nuclease cleavage patterns, restriction endo

60 chromatin nor to differences in the in vivo micrococcal nuclease cleavage sites in individual genes

61 10-bp periodicity in WW dinucleotides and in micrococcal nuclease cleavage, providing evidence for ro

62 caused by either linker histone addition or micrococcal nuclease cleavage.

63 clei sorting; 3) preparation of chromatin by micrococcal nuclease digest; 4) ChIP for open chromatin-

64 Separation of micrococcal nuclease-digested chromatin by sucrose gradi

65 Low resolution Southern analysis of micrococcal nuclease-digested chromatin from untreated r

66 We begin with micrococcal nuclease-digested non-cross-linked chromatin

67 The protection against micrococcal nuclease digestion afforded to chromatosomal

68 gradient sedimentation, thermal disassembly, micrococcal nuclease digestion and atomic force microsco

69 examined HSV-1 during lytic infection using micrococcal nuclease digestion and chromatin immunopreci

70 of S. cerevisiae nucleosome lengths based on micrococcal nuclease digestion and paired-end sequencing

71 er DNA, stabilizing an additional 20 bp from micrococcal nuclease digestion and restrict nucleosome m

72 e demonstrate by single-molecule approaches, micrococcal nuclease digestion and small-angle X-ray sca

73 e center of the DNA sequence, protected from micrococcal nuclease digestion by incorporation into a p

74 HBc 149, 154, and 157) remained intact after micrococcal nuclease digestion by native gel electrophor

75 region of the capsid pgRNA is susceptible to micrococcal nuclease digestion during its isolation and

76 replicative aging using spike-in controlled micrococcal nuclease digestion followed by sequencing.

77 ammalian linker histone H1 and have a unique micrococcal nuclease digestion footprint that allows the

78 ound histone H3 and increased sensitivity to micrococcal nuclease digestion in WHS patient-derived ce

79 Partial micrococcal nuclease digestion indicated that the sequen

80 Micrococcal nuclease digestion indicated the presence of

81 itivity of bulk chromatin from sin4 cells to micrococcal nuclease digestion is strikingly increased r

82 Micrococcal nuclease digestion of active promoters in nu

83 of genomic DNA species, produced by partial micrococcal nuclease digestion of chromatin, can be sequ

84 me-length DNA ( approximately 166 bp) during micrococcal nuclease digestion of chromatin.

85 of a 166 bp chromatosome intermediate during micrococcal nuclease digestion of chromatin.

86 itation, we developed a novel strategy using micrococcal nuclease digestion of cross-linked chromatin

87 Capsid disassembly can be induced by micrococcal nuclease digestion of encapsidated RNA.

88 445 nucleotide human telomerase RNA (hTR) by micrococcal nuclease digestion of partially purified hum

89 In this study, we use a procedure based on micrococcal nuclease digestion of reconstituted nucleoso

90 The micrococcal nuclease digestion pattern of chromatin from

91 pause at approximately 168 base pairs in the micrococcal nuclease digestion pattern of the chromatin.

92 ure on the transient template as measured by micrococcal nuclease digestion pattern.

93 rther, we found that A-T cells had different micrococcal nuclease digestion patterns compared to norm

94 structure as DNaseI-hypersensitive sites or micrococcal nuclease digestion patterns.

95 Despite these changes, the micrococcal nuclease digestion profile of this promoter

96 from the Rb-/- cells is more susceptible to micrococcal nuclease digestion than that from Rb+/+ fibr

97 the exon 1 region is much more sensitive to micrococcal nuclease digestion than the exon 2 and exon

98 A major site of micrococcal nuclease digestion was identified by mapping

99 obes were used to capture RNA targets, and a micrococcal nuclease digestion was performed to remove a

100 Similar kinetics of micrococcal nuclease digestion were seen for all three t

101 nucleosome position in follicle cells using micrococcal nuclease digestion with Ilumina sequencing.

102 and loss of a regular nucleosomal ladder on micrococcal nuclease digestion, addition of TSA relieves

103 coli chromosome in vivo and protect DNA from micrococcal nuclease digestion, allowing us to map bindi

104 leosomes, produce a chromatosome stop during micrococcal nuclease digestion, and aggregate chromatin.

105 NA and multiples of approximately 60 bp from micrococcal nuclease digestion, and immunoprecipitation

106 nucleosomes prepared by partial and maximum micrococcal nuclease digestion, coupled with Western blo

107 niques along with such laboratory methods as micrococcal nuclease digestion, predicting the genomic l

108 H3 nucleosomes protect 90-100 bp of DNA from micrococcal nuclease digestion, sufficient for only a si

109 some maps generated by chemical cleavage and micrococcal nuclease digestion, the chemical map shows c

110 Using micrococcal nuclease digestion, we probed the chromatin

111 ved from K562 human erythroleukemic cells by micrococcal nuclease digestion.

112 (HTa) that protects part of the genome from micrococcal nuclease digestion.

113 . thermophila mononucleosomes were stable to micrococcal nuclease digestion.

114 ity, and chromatin that is hypersensitive to micrococcal nuclease digestion.

115 ome segment protected by the polymerase from micrococcal nuclease digestion.

116 CTD antibodies from chromatin solubilized by micrococcal nuclease digestion.

117 chromatin results in increased resistance to micrococcal nuclease digestion.

118 In micrococcal-nuclease digestion experiments, nucleosomes

119 size differences between repeats in partial micrococcal nuclease digests and by trypsin treatment of

120 Analysis of micrococcal nuclease digests of chromatin using hybridiz

121 a typical pattern of nucleosomal repeats in micrococcal nuclease digests, the Tec element chromatin

122 by Snf-Swi, by a high resolution analysis of micrococcal nuclease digests.

123 rmal global chromatin density as assessed by micrococcal nuclease digests; and expressed normal level

124 Micrococcal nuclease, DNase I, and restriction enzymes s

125 f nuclease probes including exonuclease III, micrococcal nuclease, DNase I, and restriction enzymes.

126 e)--a protein containing five staphylococcal/micrococcal nuclease domains and a tudor domain--is a co

127 Pretreatment of nuclear extracts with micrococcal nuclease eliminated the phosphorylation of C

128 tivity () was solubilized by Triton X-100 or micrococcal nuclease extraction, whereas hTSH2B was rela

129 osomal protections in Drosophila cells using Micrococcal Nuclease followed by sequencing.

130 Micrococcal nuclease footprinting shows that after track

131 DNase I and micrococcal nuclease footprinting, of both 5' and 3' 32P

132 (1mg), and provides the option to use MNase (micrococcal nuclease) for chromatin fragmentation.

133 examined salt-soluble chromatin released by micrococcal nuclease from a 15-day-old chicken embryo er

134 letion of both tails, a lethal event, alters micrococcal nuclease-generated nucleosomal ladders, plas

135 sed sensitivity of chromatin to digestion by micrococcal nuclease; however, phosphorylation of H2A an

136 ested chromatin from untreated rats revealed micrococcal nuclease hypersensitive regions in the proxi

137 The TBP gene comprises a 220 bp micrococcal nuclease hypersensitive site corresponding t

138 ional responses to cold tend to contain more micrococcal nuclease hypersensitive sites in their promo

139 adjacent to the TATAA box and an additional micrococcal nuclease-hypersensitive site in the linker D

140 The position and intensity of micrococcal nuclease-hypersensitive sites correlate poor

141 ma regions undergoes dramatic alterations in micrococcal nuclease hypersensitivity as cells cross the

142 Both hotspot sequences were sites of micrococcal nuclease hypersensitivity in meiotic chromat

143 Micrococcal nuclease hypersensitivity in the PBRU was la

144 We report DNase I and micrococcal nuclease hypersensitivity, chromatin immunop

145 s associated with increased accessibility to micrococcal nuclease, i.e. nucleosome depletion.

146 histone promoters and transcribed regions to micrococcal nuclease, implicating UBTF1/2 in mediating D

147 The chromatin accessibility to micrococcal nuclease in the MFA2 promoter is unaffected

148 DNA and stabilize it against digestion with micrococcal nuclease, in a similar manner to histone H1.

149 fragility, manifested as high sensitivity to micrococcal nuclease, in contrast to the common presumpt

150 ith increased resistance to both DNase I and micrococcal nuclease, indicating that the silenced state

151 P knockout) brain homogenate with RNase A or micrococcal nuclease inhibited hamster but not mouse PrP

152 ocalization of Cse4 in chromatin digested by micrococcal nuclease is consistent with the potential as

153 cribe a Hi-C-based method, Micro-C, in which micrococcal nuclease is used instead of restriction enzy

154 mic DNA is either sonicated or digested with micrococcal nuclease, making it possible that current pr

155 reconstituted branch migration substrates by micrococcal nuclease mapping and exonuclease III and hyd

156 High-resolution micrococcal nuclease mapping showed that ZL0580 induces

157 Chromatin immunoprecipitation and micrococcal nuclease mapping studies reveal that Knirps

158 Micrococcal nuclease mapping studies revealed that a nuc

159 With high-resolution micrococcal nuclease mapping, we show that the HMRa locu

160 ergence between different Hi-C protocols and micrococcal nuclease (micro-C).

161 r, since the activities in the extracts were micrococcal nuclease (MN) sensitive.

162 scription elongation complex to digestion by micrococcal nuclease (MN).

163 activity of the S. aureus secreted nuclease, micrococcal nuclease (MN).

164 ribosome integrity but negatively impact the micrococcal nuclease (MNase) activity, necessitating usi

165 ly in situ single cell chromatin imaging and micrococcal nuclease (MNase) assay to show that Brd4 dep

166 ructure of chromatin in cereal species using micrococcal nuclease (MNase) cleavage showed nucleosomal

167 Chromatin immunoprecipitation (ChIP) and micrococcal nuclease (MNase) digest assays were performe

168 We combined low and high levels of micrococcal nuclease (MNase) digestion along with core h

169 accessibility of nucleosomes, as measured by micrococcal nuclease (MNase) digestion and ATAC-seq (ass

170 uch as chromatin immunoprecipitation (ChIP), micrococcal nuclease (MNase) digestion and DNase I diges

171 We performed a time-course of micrococcal nuclease (MNase) digestion and measured the

172 emodelling analysis at gene promoters, using micrococcal nuclease (MNase) digestion followed by deep

173 We have used micrococcal nuclease (MNase) digestion followed by deep

174 We introduce a metric that uses micrococcal nuclease (MNase) digestion in a novel manner

175 We have combined standard micrococcal nuclease (MNase) digestion of nuclei with a

176 age-sensitive mtDNA regions were examined by micrococcal nuclease (MNase) digestion sequencing and Ln

177 erential nuclease sensitivity assay based on micrococcal nuclease (MNase) digestion to discover open

178 al DNA was significantly less protected from micrococcal nuclease (MNase) digestion up to 6 h postinf

179 erepressed ESs show increased sensitivity to micrococcal nuclease (MNase) digestion, and a decrease i

180 for differential sensitivity of chromatin to micrococcal nuclease (MNase) digestion, we profile acces

181 Micrococcal nuclease (MNase) digests the linker to yield

182 Micrococcal nuclease (MNase) is commonly used to map nuc

183 Micrococcal nuclease (MNase) is extensively used in geno

184 Micrococcal nuclease (MNase) is widely used to map nucle

185 ely digested to mononucleosomes using either micrococcal nuclease (MNase) or caspase-activated DNase

186 re-RC) assembly, replication initiation, and micrococcal nuclease (MNase) sensitivity at different ce

187 Micrococcal nuclease (MNase) sequence-based profiles of

188 Digestion of the chromatin with micrococcal nuclease (MNase) shows a nucleosome array wi

189 ome-wide mapping of nucleosomes generated by micrococcal nuclease (MNase) suggests that yeast promote

190 method to measure chromatin accessibility to micrococcal nuclease (MNase) that is normalized for nucl

191 avage sequencing), involves targeting of the micrococcal nuclease (MNase) to a histone mark of choice

192 hEC) uses fusion of a protein of interest to micrococcal nuclease (MNase) to target calcium-dependent

193 specific and highly efficient biosensor for micrococcal nuclease (MNase), an endonuclease produced b

194 Like micrococcal nuclease (MNase), MPE-Fe(II) preferentially

195 the restriction enzymes in Hi-C assays with micrococcal nuclease (MNase), resulting in capturing nuc

196 agments caused by the known sequence bias of micrococcal nuclease (MNase), the most widely used nucle

197 p Region Capture Micro-C (RCMC) by combining micrococcal nuclease (MNase)-based 3C with a tiling regi

198 In this review, we compare the traditional micrococcal nuclease (MNase)-based approach with a chemi

199 Here, using the micrococcal nuclease (MNase)-based chromatin accessibili

200 Using micrococcal nuclease (MNase)-based chromatin occupancy p

201 We used micrococcal nuclease (MNase)-based chromatin occupancy p

202 Compared to micrococcal nuclease (MNase)-based methods that map nucl

203 bind one or both full nucleosomes that flank micrococcal nuclease (MNase)-defined nucleosome-free pro

204 Enrichment of short micrococcal nuclease (MNase)-protected DNA segments indi

205 tes at nucleotide resolution by footprinting micrococcal nuclease (MNase)-sensitive sites.

206 inaccessible to DNA-binding factors, such as micrococcal nuclease (MNase).

207 e extent to which chromatin is digested with micrococcal nuclease (MNase).

208 n these mutants has reduced accessibility to Micrococcal nuclease (MNase).

209 The micrococcal nuclease Nuc1 is one of the major S. aureus

210 chromatic sequences become hypersensitive to micrococcal nuclease, nucleoli fail to form, and transcr

211 Treatment with micrococcal nuclease of highly purified mtRNase P confir

212 products, similar to the well-characterized micrococcal nuclease of Staphylococcus aureus.

213 moderately greater sequence preference than micrococcal nuclease or DNase I, and the sites attacked

214 essed by sedimentation and by digestion with micrococcal nuclease or DNase I.

215 prepared from chromatin digested with either micrococcal nuclease or DNaseI and are restricted in the

216 deed induce sites hypersensitive to DNase I, micrococcal nuclease, or restriction enzymes on either s

217 th Cse4 and H2A are precisely protected from micrococcal nuclease over the entire CDE of all 16 yeast

218 antibody, followed by binding of a protein A-Micrococcal Nuclease (pA/MNase) fusion protein (Skene an

219 Micrococcal nuclease partial digestion generated a ladde

220 Treatment of the polyribosomes with micrococcal nuclease prior to salt extraction solubilize

221 Use of DNase I and micrococcal nuclease probes further indicated that the s

222 DNase I and micrococcal nuclease probes show that GATA-1 binding cau

223 nt stagger is explained by the finding that micrococcal nuclease produces NCPs not with flush ends,

224 Micrococcal nuclease protection analysis showed that les

225 d Sik1 promoter accessibility as measured by micrococcal nuclease-quantitative PCR and impaired histo

226 ich antibody-targeted controlled cleavage by micrococcal nuclease releases specific protein-DNA compl

227 ent protein-tagged H1 variants, we show that micrococcal nuclease-resistant chromatin is specifically

228 ift assay, this protein produced a discrete, micrococcal nuclease-resistant complex with an approxima

229 Characterization of the most micrococcal nuclease-resistant DNA indicates that micron

230 visiae, obtained by paired-end sequencing of micrococcal nuclease-resistant DNA.

231 s that the centromeric nucleosome contains a micrococcal nuclease-resistant kernel of 123-135 bp, dep

232 ementing activity was protease-sensitive and micrococcal nuclease-resistant, had a native molecular m

233 This Asf1p/H3/H4 complex generated micrococcal nuclease--resistant DNA in the absence of DN

234 be produced by pretreatment with DNase I or micrococcal nuclease, respectively.

235 table for analysis of chromatin structure by micrococcal nuclease, restriction endonuclease or by imm

236 this probe by the secreted S. aureus enzyme micrococcal nuclease results in emission of a readily de

237 ive and inactive CEN chromatin digested with micrococcal nuclease revealed that periodic nucleosome a

238 e of exons 1, 2, and 5 of the DHFR gene with micrococcal nuclease revealed that the exon 1 region is

239 der ( 150 bp) NDRs instead contain unstable, micrococcal nuclease-sensitive ("fragile") nucleosomal p

240 nctional significance of this association, a micrococcal nuclease-sensitive component, i.e., an snRNP

241 PELP1 overexpression increased the micrococcal nuclease sensitivity of estrogen response el

242 he human protamine cluster were subjected to Micrococcal Nuclease-seq.

243 This protocol describes single-cell micrococcal nuclease sequencing (scMNase-seq), a method

244 mics simulations based on publicly available micrococcal nuclease sequencing data for nucleosome posi

245 erstood, including several studies utilizing micrococcal-nuclease sequencing (MNase-seq) methodologie

246 Micrococcal nuclease studies revealed that TRs are embed

247 Consistently, micrococcal nuclease susceptibility analysis revealed al

248 pressed CFS, the FRA3B, is more resistant to micrococcal nuclease than that of the flanking non-fragi

249 Consistently, the accessibility of micrococcal nuclease to chromatin was generally inhibite

250 a cell extracts, which were pre-treated with micrococcal nuclease to degrade the endogenous RNase P R

251 riments where the extracts were treated with micrococcal nuclease to digest endogenous snRNAs, the ef

252 acO/GFP-LacI plants were lysed, treated with micrococcal nuclease to digest the DNA to fragments of a

253 one-DNA complexes using an antibody-targeted micrococcal nuclease to release DNA complexes for paired

254 Micrococcal nuclease-treated cell extracts are fully com

255 Using micrococcal nuclease-treated chromatin, we find that Cse

256 ng to successive salt-extracted fractions of micrococcal nuclease-treated Drosophila chromatin.

257 by incubating human 32P-labeled U2 snRNP in micrococcal nuclease-treated HeLa nuclear extracts, foll

258 Addition of DNA to micrococcal nuclease-treated samples restored interactio

259 Micrococcal nuclease-treated TAP preparations were devoi

260 that the 3' ends of capsid pgRNA isolated by micrococcal nuclease treatment are heterogeneously dispe

261 ted by using an established method involving micrococcal nuclease treatment demonstrated reduced leve

262 as not bridged by nucleic acids, as shown by micrococcal nuclease treatment of the proteins prior to

263 A (vRNA) has been depleted by treatment with micrococcal nuclease, was used to study transcription in

264 By fusing a subunit of Mcm to micrococcal nuclease, we previously showed that known or

265 By using micrococcal nuclease, which has both endo- and exo-nucle

266 eosome cores were liberated using an enzyme (micrococcal nuclease) with a strong preference for cleav