ライフサイエンスコーパス: microinjection

1 lcium-ionomycin, ionomycin, or hPLCzeta cRNA microinjection.

2 mprovement in embryo viability compared with microinjection.

3 ith high efficiency and controlled volume is microinjection.

4 7/4-1BB) directly in the NOD mouse by embryo microinjection.

5 e aversion was blocked by intra-mPFC nor-BNI microinjection.

6 eters and synchronization on the efficacy of microinjection.

7 e immaturity of the gonad at the time of the microinjection.

8 ction station to confine droplets during the microinjection.

9 ith the needs of high-throughput (HT) serial microinjection.

10 degree of mosaicism when compared to zygote microinjection.

11 ative to the conventional delivery method of microinjection.

12 DHK also enhanced responses to glutamate microinjection.

13 d protocols which require electroporation or microinjection.

14 Microinjections (1 mul) of L-732138 (50 nm) and SB222200

15 egulatory cold defense, by means of repeated microinjections (100 nl) of the GABA(A) agonist muscimol

16 Second, OVLT microinjection (20 nl) of 1.0 m NaCl significantly raise

17 ly: for 24 h immediately following a control microinjection and for an additional 24h after a second

18 We then tested enriched features by embryo microinjection and functional tests of multiple protein

19 ploid Xenopus laevis that is widely used for microinjection and tissue explant-based protocols, and t

20 SD in murine hippocampal slices by focal KCl microinjection and visualized the ensuing beading of den

21 verexpression mouse model 4 weeks after aSyn microinjections and after the onset of symptomatic forep

22 reticulospinal cells responded to glutamate microinjections and the size of the responses paralleled

23 recombination (HR) template vectors, embryo microinjection, and detection of mutations and insertion

24 transgene expression with classic pronuclear microinjection, and it offers comparable efficacies (num

25 transgene expression with classic pronuclear microinjection, and it offers comparable efficacies to l

26 very favourably with conventional pronuclear microinjection, and report an improvement in mutagenesis

27 imaging, immunohistochemistry, AAV-FLEX-GFP microinjections, and crosses to RiboTag, Ai95, and new C

28 l half of medial shell, where opioid agonist microinjections are known to enhance positive hedonic or

29 nt to sustain whole-body general anesthesia; microinjection as little as 0.5 mm off-target did not.

30 eins can be confirmed by using CyaA, GSK, or microinjection assays.

31 bove antinociceptive effect was abolished by microinjection (at RAIC) of the following: (1) L-368899

32 arget activity in cells could be achieved by microinjection compared with nucleofection.

33 Microinjection coupled with confocal microscopy was used

34 Moreover, BDNF microinjection directly into the VMN also lowered fastin

35 The behavioral effects of NMU microinjection directly to the NAcSh were investigated u

36 s delivered to zebrafish through caudal vein microinjection during distinct periods in early neurodev

37 dopted in laboratories lacking sophisticated microinjection equipment, and can be implemented by rese

38 In a zebrafish microinjection experiment, dre-miR-140-5p is shown to ex

39 uronal anatomical tracing, intrahypothalamic microinjections, extracellular single-unit recordings of

40 Finally, estradiol microinjections followed by microdialysis were used to d

41 ghly efficient with the potential to replace microinjection for in vivo genome editing in mice and po

42 at excitation at the same site prevents DNQX microinjections from recruiting downstream limbic struct

43 Kif2a depletion by siRNA microinjection generated severely defective spindles and

44 After microinjection, glomeruli were capable of engrafting on

45 ques such as electroporation, lipofection or microinjection have been developed to overcome the cellu

46 In naive animals, OXT microinjection in the dorsal vagal complex induced a NO-

47 loped methods for gene transduction by viral microinjection in the epithelium of cultured Neurog3-nul

48 ring localized vascular injury with thrombin microinjection in the mesenteric circulation of mice, we

49 ity were recorded after N-methyl-d-aspartate microinjection in the SNpc and/or optogenetic stimulatio

50 ameters were significantly influenced by the microinjections in a biphasic dose-response relationship

51 urrent study examined the effects of similar microinjections in euthyroid rats.

52 We used simultaneous microinjections in medial prefrontal cortex regions and

53 nal inhibition is required for AMPA-blocking microinjections in medial shell to induce either positiv

54 Microinjections in nucleus accumbens of glutamate antago

55 ion and for an additional 24h after a second microinjection including a T3 dose to the preoptic regio

56 rmic (30 degrees C) culture of zygotes after microinjection increased HDR efficiency for some loci.

57 Here, we describe a novel and simple microinjection-independent technique, called Genome-edit

58 expression selectively in the sMic lineage: microinjection into a Sp fertilized egg of an RNA that c

59 rapid and potent killing phenotype following microinjection into an insect host.

60 plets and manage rapid and highly controlled microinjection into droplets.

61 d in male Wistar rats the effect of oxytocin microinjection into RAIC during an inflammatory (by subc

62 emonstrates that TALE nuclease and donor DNA microinjection into rat zygotes results in efficient and

63 ted abstinence was prevented by pioglitazone microinjection into the amygdala.

64 int stress and nonrestraint rats after N/OFQ microinjection into the CeA.

65 with the LY341495, both systemically and via microinjection into the medial prefrontal cortex (mPFC),

66 geting MALAT1 RNA, delivered by transuterine microinjection into the mouse amniotic cavity at embryon

67 st treatment, by administering an additional microinjection into the mPFC immediately prior to ED tes

68 Lastly, using FluoroGold microinjection into the NG, we found that the existence

69 ZIKV microinjection into the somatosensory cortex on one side

70 reduced rhythmic burst amplitude after AMPA microinjection into the XIIMN.

71 We applied TALE nucleases and donor DNA microinjection into zygotes to generate HDR-modified rat

72 Site-specific microinjections into M1 demonstrated that l-DOPA-induced

73 withdrawal behavior is absent with analogous microinjections into the lateral habenula of nicotine-tr

74 We used in vivo microinjections into the preBotC and in vitro isolated b

75 food-seeking experiments indicate that PEPA microinjections into the RMTg did not influence the exti

76 However, microinjection is a technically demanding, labor-intensi

77 Microinjection is an effective actuation technique used

78 Microinjection is considered the gold standard technique

79 Finally, scopolamine microinjections localized to the caudal half of medial s

80 ng rats of both sexes, we applied a modified microinjection method that permitted localization of the

81 In this study, we report an efficient microinjection method to generate A. phagocytophilum-inf

82 Finally, the feasibility of implementing our microinjection model is examined experimentally.

83 jor subtypes of opioid receptors via agonist microinjections [mu (DAMGO), delta (DPDPE), or kappa (U5

84 ipped with epifluorescence and injected with microinjection needles using a picospritzer forced-air i

85 g in the contralateral hemisphere, after the microinjection of 10 mug of LPS.

86 ditional knockout (cKO or floxed) alleles by microinjection of 2 single guide RNAs (sgRNA) and 2 sing

87 Bilateral microinjection of [Pyr(1) ]apelin-13 into the rostral ve

88 Bilateral RVLM microinjection of [Pyr(1) ]apelin-13 significantly incre

89 For Cre lines generated via pronuclear microinjection of a Cre transgene construct, the integra

90 Importantly, microinjection of a Cre-inducible ErbB4 virus (AAV-ErbB4

91 This technique involved the microinjection of a fluorescent phosphopeptide that is h

92 a 1.8 kb gene was contrasted when combining microinjection of a gRNA/Cas9 ribonucleoprotein complex

93 genome editing is typically accomplished by microinjection of a mixture of Cas9 DNA/mRNA and single-

94 We previously demonstrated that transuterine microinjection of a splice-switching antisense oligonucl

95 Using microinjection of adeno-associated viral vector bearing

96 Conversely, microinjection of adults with the phosphaturic human hor

97 In addition, microinjection of AIP into the PVN significantly reduced

98 ric measurement of heart rate, indicate that microinjection of aldosterone into the nucleus ambiguus

99 1 antibody, depolymerizing microtubules, or microinjection of an antibody that inhibits kinesin, VE-

100 Furthermore, bilateral microinjection of ANA-12 into the dmNTS greatly diminish

101 aIII spectrin using siRNA technology and the microinjection of anti-betaIII spectrin antibodies into

102 Bim silencing or microinjection of anti-Bim antibodies into the cell cyto

103 Accordingly, local microinjection of anti-MFG-E8 mAb exacerbated periodonta

104 Tissue-targeted loss-of-function assays (via microinjection of antisense morpholino or CRISPR-Cas9) c

105 Moreover, microinjection of artificial cerebrospinal fluid at a pH

106 d completely prevented it when combined with microinjection of autophagy-targeting antibodies specifi

107 Conversely, in AP rats, microinjection of bicuculline had no effect, whereas kyn

108 In control rats, microinjection of bicuculline into the DMV increased PES

109 genome editing is typically accomplished by microinjection of Cas9 DNA/RNA and single guide RNA (sgR

110 rate of Fah gene targeting was achieved with microinjection of Cas9 mRNA, gRNA and single strand olig

111 Cdc23 localized on the meiotic spindle, and microinjection of Cdc23 siRNA caused decreased ratios of

112 Microinjection of CelTOS into cells resulted in observab

113 Manipulation of the 5-HT system through microinjection of compounds suggests its involvement in

114 of pancreatogenesis-disabled sheep by oocyte microinjection of CRISPR/Cas9 targeting PDX1, a critical

115 In current clamp recordings, microinjection of cross-linked 300 kDa increased excitab

116 In addition, microinjection of D-serine into the SCN also increased C

117 We demonstrate that microinjection of DNA constructs into fertilized one-cel

118 n contrast, no effect was observed following microinjection of doses that are not thought to block as

119 Microinjection of double-stranded RNAs targeting ku70 or

120 Using in vivo microdialysis and microinjection of drugs into the mesolimbic DA system, w

121 Using in vivo microdialysis and microinjection of drugs into the mesolimbic dopamine sys

122 ht to block astrocytic channels or following microinjection of either dose into the nucleus accumbens

123 We describe a protocol for microinjection of embryos for an emerging model system,

124 We show that microinjection of endothelial cells with a monoclonal an

125 tivation of VMH EphA5 receptors via targeted microinjection of ephrinA5-Fc before a hyperinsulinemic

126 rague-Dawley rats that received an acute VMH microinjection of ephrinA5-Fc, chronic VMH knockdown, or

127 We recently reported that microinjection of ethanol into the rostral ventrolateral

128 ng >3 weeks was observed following bilateral microinjection of FGF1 into the ARC-ME.

129 frogs, drug inhibition or overexpression by microinjection of formin has a chirality-randomizing eff

130 Remarkably, microinjection of free lysine (K) 63-linked ubiquitin ch

131 Microinjection of GABA(A) receptor antagonists into PnO

132 r 3 weeks abstinence was increased following microinjection of gap-junction hemichannel blockers into

133 e achieved in human cells using glass-needle microinjection of genome editing reagents.

134 Bilateral microinjection of glycine into the SCN elevated CBF in a

135 xtracellular administration or intracellular microinjection of GPR55 ligands.

136 Microinjection of high concentrations of the peptide GsM

137 n days later, mice received intradermal (id) microinjection of histamine, chloroquine, capsaicin, or

138 ble to transmit infection experimentally via microinjection of homogenate from these galbut-only flie

139 the defect in motility, were rescued both by microinjection of human ERBB4 mRNA and by transposon-med

140 Microinjection of ICI 174,864, a delta-opioid receptor a

141 ssion and knockdown of gene function through microinjection of in vitro-translated mRNAs or gene-spec

142 novel in vivo chemotaxis assay, perivenular microinjection of inflammatory mediators induced directi

143 CSD, elicited by pressure microinjection of KCl, was recorded in anesthetized rats

144 Microinjection of ketamine into the prelimbic (PL) regio

145 Microinjection of l-DOPA directly into the striatum amel

146 e induced in the rat spinal dorsal column by microinjection of lipopolysaccharide, and examined immun

147 In addition, microinjection of losartan or chelerythrine into the PVN

148 s the Ca(2+) signal induced by intracellular microinjection of LPI converges to hyperpolarization of

149 In vivo a single intravitreal microinjection of mAbFzd7 or CRD significantly attenuate

150 Second, in rats, we demonstrated that the microinjection of MCH into the lateral ventricle results

151 Microinjection of MECP2-targeting TALEN plasmids into rh

152 Conversely, microinjection of MFG-E8 inhibited bone loss in experime

153 Intraspinal stereotaxic microinjection of MIF resulted in upregulation of inflam

154 Finally, microinjection of miR-153 in the region of mouse first m

155 Co-microinjection of mouse embryos with Cas9 mRNA and singl

156 e human embryonic stem cell-based system and microinjection of mouse zygotes.

157 the CeA or BLA in macaques by intracerebral microinjection of muscimol (to inactivate) or bicucullin

158 ssium currents were significantly reduced by microinjection of mutant G85R SOD1YFP that had been prei

159 nic technology such as isolation of zygotes, microinjection of NAs into them, and their subsequent tr

160 stimulation of mossy fibers (MFs) as well as microinjection of NMDA in the granular layer generates b

161 tic tone and sympathoexcitatory responses to microinjection of NMDA in the PVN of rats with CHF.

162 Here, we show that NAc microinjection of orexin-A in medial shell amplifies the

163 o stimulate in vivo tumor growth early after microinjection of OVCAR3 cells in nude mice.

164 Following CRF pretreatment, however, microinjection of OXT attenuated or, at times reversed,

165 Microinjection of OXT in the DVC decreased gastric tone

166 Additionally, intracerebral microinjection of pathologic tau led to increased tau ag

167 Microinjection of pioglitazone into the AMY, but not int

168 Consistent with this finding, site-specific microinjection of pioglitazone into the RMTg but not int

169 Systemic or local microinjection of PKR inhibitor to the gustatory cortex

170 Importantly, localized microinjection of PMN-MPs into wounded colonic mucosa wa

171 harvesting embryos from one set of females, microinjection of reagents into embryos ex vivo and thei

172 Microinjection of RO5166017 into the NAc core and shell

173 The results showed that microinjection of RO5166017 into the VTA and PrL decreas

174 nforced operant responding was unaffected by microinjection of RO5166017 into these brain regions.

175 Whereas bilateral intra-arcuate microinjection of saline vehicle was without effect, rem

176 ree of cue-induced remifentanil seeking, and microinjection of SB into VP attenuated this behavior wi

177 Indeed, we show here that microinjection of single-guide RNA/Cas9 ribonucleoprotei

178 uppression of Ku70 concurrent with embryonic microinjection of site-specific nucleases yielded consis

179 ction of an anesthesia-like state in rats by microinjection of small amounts of GABAA-receptor agonis

180 Microinjection of small amounts of human full-length DUX

181 Microinjection of somatostatin (SST) causes site-specifi

182 Microinjection of sperm tsRNAs from the F1-HFD male into

183 Building on prior research, this study used microinjection of synthetic miR-310s mimics into DDT-res

184 ibility of achieving targeted mutagenesis by microinjection of TALEN mRNA within the mouse oocyte.

185 In contrast, intra-VTA microinjection of tatCN21 just before the CPP test did n

186 Conversely, microinjection of the adenylyl cyclase inhibitor SQ22536

187 Microinjection of the agonists (MT-II and alpha-MSH) int

188 Here, we show that microinjection of the AMPAR antagonist NBQX into the NAc

189 on also decreased antinociception induced by microinjection of the GABAA receptor antagonist bicucull

190 Microinjection of the glutamate antagonist, kynurenic ac

191 odes could detect brain NO released by local microinjection of the glutamatergic agonist N-methyl-d-a

192 Microinjection of the glycine receptor antagonist strych

193 Here we show that microinjection of the glycosyltransferase domain Afp18(G

194 Significantly, microinjection of the HCN blocker ZD7288 into the ACC in

195 ffect of donepezil was markedly reduced by a microinjection of the M2 antagonist, methoctramine, with

196 spring stress responsivity and, using zygote microinjection of the nine specific miRs, demonstrated a

197 WKY rats, and this effect was eliminated by microinjection of the NMDAR antagonist into the PVN.

198 Microinjection of the PLCzeta EF-hand mutants into mouse

199 However, microinjection of the progesterone receptor antagonist,

200 Two patients had undergone microinjection of the same branded topical moisturizer (

201 Combined with our finding that bilateral microinjection of the same dose of FGF1 into the PVN was

202 Microinjection of the vectors into zygotes and transfer

203 Microinjection of ticks with IFNgamma induced IGTPase, a

204 or the production of mouse disease models by microinjection of transcription activator-like effector

205 Microinjection of tumor necrosis factor alpha (TNFalpha)

206 sicular stomatitis virus (VSV), we show that microinjection of VSV particles leads to a dose-dependen

207 Microinjection of Wnt8 and Wnt11 mRNA induced ectopic do

208 Pronuclear microinjection of ZFNs, shown by our data to be an effic

209 eration of tissue-specific knockout rats via microinjection of zinc-finger nucleases (ZFNs) into fert

210 Following microinjection of zygotes, resulting morphant larvae wer

211 glutamate receptors within the same region, microinjections of 1 mum substance P or 1 mm nicotine in

212 privation in the PeH or RVLM was elicited by microinjections of 2-deoxy-D-glucose or 5-thio-D-glucose

213 Microinjections of a D1 antagonist in the MLR decreased

214 Microinjections of a glutamate AMPA antagonist (DNQX) in

215 odor during sleep was preceded by bilateral microinjections of a protein synthesis inhibitor into th

216 n (STZ)-diabetic rats received bilateral VMH microinjections of an adenoassociated viral vector conta

217 easured BRS before and after bilateral dmNTS microinjections of ANA-12.

218 The present report describes the results of microinjections of anterograde tracers placed at differe

219 Respiratory responses evoked by microinjections of capsaicin into the paratrigeminal nuc

220 n CFA-treated females that was reversed with microinjections of DS2 directly into the vlPAG.

221 Intrasubthalamic microinjections of either an inverse agonist of D5Rs, fl

222 Results showed that lesions of the mPOA or microinjections of estradiol directly into the mPOA incr

223 We gave hippocampal microinjections of FKBP1b-expressing viral vector to mal

224 Microinjections of fluorescent tracers, along with gold-

225 In contrast, microinjections of glutamate agonists enhanced the respi

226 Microinjections of glutamate and muscimol to activate or

227 Bath-application or local microinjections of glutamatergic antagonists markedly re

228 e nucleus (KF) were inhibited with bilateral microinjections of isoguvacine (50-70 nl, 10 mm) to remo

229 uculline into the DMV increased PES, whereas microinjections of kynurenic acid had no effect.

230 orated the dystonic movements but cerebellar microinjections of l-DOPA had no effect.

231 Previous work has shown that acute microinjections of l-triiodothyronine (T3) to the preopt

232 Unilateral microinjections of lidocaine, muscimol, or glutamate ant

233 Previously, we demonstrated that microinjections of MCH into the DRN resulted in an incre

234 Furthermore, intra-VTA microinjections of mGluR1 antagonist JNJ16259685 and pro

235 Repeated microinjections of morphine or the adenylyl cyclase acti

236 We examined whether microinjections of orexin-A into the VP hotspot enhance

237 Microinjections of pioglitazone in the ventral tegmental

238 Hippocampal microinjections of pioglitazone reduce the insurgence of

239 Hippocampal microinjections of pioglitazone reduced the expression o

240 ty in the hippocampus was modulated by focal microinjections of potassium chloride into the nucleus r

241 Microinjections of SB-334867 (20 nmol) bilaterally into

242 We report that intra-VTA microinjections of tatCN21 before cocaine conditioning b

243 cquisition of cocaine CPP, whereas intra-VTA microinjections of tatCN21 before saline conditioning di

244 Pre- or post-training intra-RMTg microinjections of the allosteric AMPA receptor potentia

245 In contrast, microinjections of the antagonist scopolamine at all sit

246 Prior to reinstatement, rats received microinjections of the GABA agonists baclofen/muscimol (

247 Microinjections of the GABAA agonist muscimol into PVN i

248 DMV microinjections of the group II mGluR agonist APDC and w

249 Microinjections of the KCa2 channel inhibitor apamin int

250 Intra-VP microinjections of the OxR1 antagonist SB-334867 (SB) de

251 l economics procedure, we show that intra-VP microinjections of the OxR1 antagonist SB-334867 decreas

252 ant P450s, the effects of intracerebral (ic) microinjections of the P450 inhibitor CC12 were determin

253 Brain site-specific microinjections of the PPARgamma agonist pioglitazone we

254 layed sensitivity to capsaicin and brainstem microinjections of these neuropeptides induce GI effects

255 development of morphine tolerance, and vlPAG microinjections of TLR4 agonists dose dependently produc

256 one and the gastric motility response to DMV microinjections of TRH were decreased significantly.

257 ciceptive neurons were used to guide precise microinjections of various tracers in VMpo.

258 Adult male rats received bilateral microinjections of vector control or short hairpin RNA t

259 Male Long-Evans rats received intra-BLA microinjections of viral vectors carrying either halorho

260 Next, we evaluated the effects of NMU microinjection on behavioral sensitization to cocaine.

261 e transgene expression to classic pronuclear microinjection or somatic cell nuclear transfer (SCNT),

262 fluorescent vesicles (e.g., electroporation, microinjection, or membrane transduction peptides).

263 approximately ten times smaller than typical microinjection pipettes and rather than pressure pulses

264 The catheters were sealed with microinjection ports and then implanted subcutaneously.

265 versely, CeA inhibition by muscimol/baclofen microinjections prevented acquisition of cocaine self-ad

266 Oxytocin microinjection produces a diminution of (1) flinches ind

267 MII oocyte microinjection reduced lysis, improved blastocyst rate,

268 I depolarizes PAG neurons and upon intra-PAG microinjection, reduces nociceptive threshold in the hot

269 Furthermore, electroporation, compared to microinjection, results in a higher rate of embryo survi

270 Moreover, blastocyst microinjection showed that the iMuSCs contributed to chi

271 In 7 rats with histologically confirmed microinjection sites bilaterally placed in the preoptic

272 By contrast, microinjection sites in the anterior half of VP, or in L

273 at all sites throughout medial shell, orexin microinjections stimulated 'wanting' to eat, as reflecte

274 We present a new passive microinjection technique relying on pressure-driven flui

275 Several microinjection techniques have been developed for actuat

276 Comparative In Vivo Oncology (CIVO) arrayed microinjection technology to test tumor responsiveness t

277 in, which was otherwise elevated by NAc DNQX microinjections that generated motivation.

278 e targeted CpG methylation in mice by zygote microinjection, thereby demonstrating its potential util

279 Since it does not require electroporation or microinjection, this tool has the potential to be applie

280 We used microinjection to infect the organoids with H pylori.

281 The MgRA also guides the real-time microinjection to specific deep brain nuclei, which is d

282 bility of NAc core GLP-1R activation by Ex-4 microinjection to suppress food intake and body weight g

283 Furthermore, NMU microinjection to the NAcSh decreased local gamma-aminob

284 al time of 5-HT and noradrenaline applied by microinjection to the NTS.

285 These effects of T3 microinjection to the preoptic region were demonstrated

286 effectively reversed the ability of NAc DNQX microinjections to generate appetitive motivation, and s

287 tion, and similarly reversed ability of DNQX microinjections to generate defensive motivation.

288 inhibition is in fact necessary for NAc DNQX microinjections to generate motivations.

289 We performed fluorescent dye microinjections to identify aortic arch patterns and mea

290 tions at the same local sites in NAc as DNQX microinjections to oppose relative neuronal inhibitions

291 grade tracing with fluorescent intracellular microinjections to perform three-dimensional reconstruct

292 onal inhibition in NAc is necessary for DNQX microinjections to produce either desire or dread.

293 ing-free single-guide RNA (sgRNA) synthesis; microinjection; validation of the target-specific activi

294 by combining liposome-based technology with microinjection, we were able to establish a wide range o

295 Minimal effects of lateral ventricle T3 microinjection were demonstrated (N=5).

296 orts to transmit SmedTV horizontally through microinjection were unsuccessful.

297 Microinjections were paired, one made during pacing to m

298 ntagonist, SAFit2, in wild-type mice via BLA microinjections, which reduced anxiety-related behavior.

299 termed injectoporation that combines tissue microinjection with electroporation to express cDNAs and

300 As predicted, microinjection with unmodified or lesion-containing CRE,