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1 ent as stored mRNA and stored protein in the microspore.
2 the highest levels in developing ovules and microspores.
3 the tetrad microspores had become individual microspores.
4 type-specific pectin degradation to separate microspores.
5 ce of storage oil bodies inside the maturing microspores.
6 te with the cell wall of aberrant developing microspores.
7 d, vacuolated tapetum surrounding collapsing microspores.
8 ating protein transport in tapetal cells and microspores.
9 nd is precisely regulated by the tapetum and microspores.
10 , with corresponding loss of fluorescence on microspores.
11 onads that contain binuclear and polynuclear microspores.
12 ll be transferred to the surface of adjacent microspores.
13 ical system of mesopores interconnected with microspores.
14 resulting in the formation of multinucleate microspores.
15 rived siRNAs were depleted in Capsella nrpd1 microspores.
16 lted in polyads containing from one to eight microspores.
17 mutants is attributed to the degeneration of microspores.
18 len, but were already present in unicellular microspores.
21 er, it does not appear to be anchored to the microspore and forms large aggregates on the developing
22 ment, we identified genes involved in pollen microspore and tapetum development that were specificall
25 uorescent protein accumulated in unicellular microspores and bicellular pollen but decreased in trice
26 porter gene and selectable marker into these microspores and hence, after in vitro maturation and in
28 isms by which sporopollenin is anchored onto microspores and polymerized in specific patterns are unk
29 methylation is lost from retrotransposons in microspores and sperm cells and restored by de novo DNA
30 t in the surface fractions of the developing microspores and the mature pollen, although fragmented o
31 e cell, and their precursor, the postmeiotic microspore, and found that unlike in mammals the plant g
32 primary cell wall of meiocytes, tetrads and microspores, and the expression of this gene is essentia
35 nt cell wall matrix formed on the surface of microspores at the late tetrad stage, is hypothesized to
36 tum of 3-5 mm B. napus buds, which contained microspores at the late-vacuolate and bicellular stages
37 Enzymatic removal of callose from wild-type microspores at the tetrad stage did not release the micr
43 chemical and genetic tools on Brassica napus microspore-derived embryos and Arabidopsis thaliana zygo
44 icate that RGP1 and RGP2 are required during microspore development and pollen mitosis, either affect
48 of PSP1 in the tapetum at critical stages of microspore development suggests that PSP1 activity in th
49 l staining and thin sectioning revealed that microspore development was compromised, likely due to a
50 f substances, analogous to those involved in microspore development, were left undisturbed while wate
62 occurs in the microsporocyte to produce four microspores, each of which develops into a pollen grain.
63 he products of male meiosis into a tetrad of microspores, each of which develops into a pollen grain.
64 genesis in dandelion (Taraxacum officinale), microspore embryogenesis in oilseed rape (Brassica napus
65 e investigated this using the Brassica napus microspore embryogenesis system, where the male gametoph
68 Pol IV is functionally required in Capsella microspores emphasizes the relevance of investigating di
69 key role in the developmental progression of microspores, enabling formation of the two male cell lin
72 sors are secreted from the tapetum to become microspore exine constituents; this pathway explains the
73 psis thaliana produce tetrad pollen in which microspores fail to separate during pollen development.
75 the stress during the stages of meiosis and microspore formation but had no effect on more advanced
77 Here we develop a method to isolate the four microspores from a single tetrad in maize for the purpos
78 nd vegetative cells, as well as in wild-type microspores from which both pollen cell types originate,
81 ly of exine components from tapetal cells to microspores in the intact anthers of Arabidopsis thalian
85 use the amount of callose deposition between microspores is correlated with tetrad pollen formation i
87 nt embryos can develop from somatic cells or microspores, maternal contributions are not considered t
91 iggering microRNAs (miRNAs) are localized in microspore mother cells and tapetal cells of meiotic and
92 meiotic cytokinesis in tes mutants, all four microspore nuclei remain within the same cytoplasm, with
94 usion of nuclei in binuclear and polynuclear microspores occurs spontaneously before pollen mitosis I
95 of the GUS reporter gene in the tapetum and microspores of Arabidopsis anthers identical to the AtMY
97 ation of the callose wall that separates the microspores of the tetrad, and also play a gametophytic
102 ctive pollen wall formation such as abnormal microspore plasma membrane undulation and defective prim
104 this gene was only expressed in embryogenic microspores, pollen embryoids, and developing zygotic em
105 t are differentially regulated in developing microspores/ pollen grains (gametophyte) and tapetal cel
109 Degeneration of pollen occurs soon after microspore release from the tetrads, at which time the t
110 ts, pollen development is aborted soon after microspore release, regardless of environmental conditio
115 TET loci in Arabidopsis result in failure of microspore separation during pollen development due to a
120 ed proteins in the tapetal cells at the free microspore stage, contributing to pollen wall developmen
123 lls for synthesis of compounds important for microspore structure and in transfer of organic nitrogen
124 er cell layers surrounding the meiocytes and microspores, suggesting that appropriate GA signaling in
125 ores at the tetrad stage did not release the microspores, suggesting that callose removal is not suff
129 plast microtubules during cytokinesis in the microspore that is essential for cell plate formation.
132 tisense RNA approach that it is required for microspores to progress from the unicellular to bicellul
133 on in all transgenic plants regenerated from microspores transfected with the full transferred DNA/pr
137 aging of GFP-tagged microtubules in dividing microspores we show that TIO is required for expansion o
138 rescent components of developing tapetum and microspores were imaged in intact, live anthers using tw
140 dified form, then relocate to the developing microspores where they eventually constitute some of the
143 combination protein A delivered to triticale microspores with the help of a Tat2 cell-penetrating pep
144 ad previously been shown to produce abnormal microspores with variable DNA content, was also cytologi