ライフサイエンスコーパス: microtubule

1 s organize mitotic spindles by sliding apart microtubules.

2 acent to NE holes containing meiotic spindle microtubules.

3 ly of old microtubules and nucleation of new microtubules.

4 -range organelle dispersion in opposition to microtubules.

5 migration to a similar extent as disrupting microtubules.

6 chinery that connects chromosomes to spindle microtubules.

7 trials, kills cancer cells by destabilizing microtubules.

8 lational modification enriched on long-lived microtubules.

9 f tau from and subsequent destabilization of microtubules.

10 cellular transport towards the minus-ends of microtubules.

11 ubulin nanobody specific towards tyrosinated microtubules.

12 s into a helical geometry poised to nucleate microtubules.

13 regulating the lateral stability of cortical microtubules.

14 (CCDC66) and TOG array regulator of axonemal microtubules 1 (TOGARAM1) as ARMC9 interaction partners.

15 cross-linking and sliding by decreasing the microtubule affinity of the XCTK2 tail domain.

16 nts of cytoskeletal networks, and changes in microtubules and actin filaments are well studied.

17 Two major cytoskeletal components, microtubules and actin filaments, together with a microt

18 nd post-division migrations are dependent on microtubules and actin, respectively, and the polarity c

19 the contact site leads to reorganization of microtubules and associated organelles.

20 dicate a strategy of pollen tubes to protect microtubules and avoid growth arrest involved in sexual

21 Superresolution imaging of microtubules and clathrin-coated pits was demonstrated,

22 This localization requires microtubules and coincides with sites of high laminin co

23 l and fragmented and translocate apically on microtubules and distribute progressively along the cell

24 the Dam1c to increase its residence time on microtubules and enhance kinetochore-microtubule attachm

25 This SPB also contains relatively fewer microtubules and less endogenous Spc110.

26 agent-based simulations with elastic dynamic microtubules and molecular motors.

27 Perturbations of microtubules and motor proteins disrupt this sequence of

28 This remodeling involves disassembly of old microtubules and nucleation of new microtubules.

29 ginine-rich sequence within the LCD binds to microtubules and targets condensation of LEM2 to spindle

30 ur results revise the current viewpoint that microtubules and their associated proteins are the only

31 However, dynamic properties of the microtubule are key to its function, and this behavior h

32 Microtubules are dynamic tubulin polymers responsible fo

33 le nucleation pathways and helps explain how microtubules are generated in the spindle.

34 in, consistent with the possibility that the microtubules are important for furrow formation.

35 luorescently tagged EB1 protein to show that microtubules are still associated with the furrows in th

36 Microtubules are tubular polymers with essential roles i

37 ning in wild-type plants, but does not cause microtubule array reorganization in the afb4-8 afb5-5 mu

38 role for PAR-6 in organizing non-centrosomal microtubule arrays in the epidermis.

39 -enter cell division by establishing mitotic microtubule arrays.

40 identified posttranslationally detyrosinated microtubules as a source of viscoelasticity in cardiomyo

41 s-end-directed processive motility on single microtubules as individual homodimers.

42 l modifications indicative of highly dynamic microtubules (as opposed to stable microtubules) within

43 Microtubules assembled from artificial centrosomes in mi

44 h as the mDIA subfamily, and promotes stable microtubule assembly.

45 membrane-associated Ankyrin (UNC-44) and the microtubule-associated CRMP (UNC-33).

46 ream from and is negatively regulated by the microtubule-associated deacetylase HDAC6, which function

47 ferroptotic 15LO1 and the autophagic protein microtubule-associated light chain-3 (LC3).

48 Surprisingly, we find that the classically microtubule-associated Pavarotti binds directly to actin

49 Microtubule-associated protein (MAP) 2 has been perceive

50 uction and mitochondrial damage, and reduced microtubule-associated protein 2 (MAP2) dendrites in hum

51 A new in vitro study finds that the microtubule-associated protein CLASP repairs lattice dam

52 al integrity and increased expression of the microtubule-associated protein light chain 3 (LC3), the

53 ing frame 72 (C9orf72), progranulin (GRN) or microtubule-associated protein tau (MAPT) and their firs

54 consisting primarily of hyperphosphorylated microtubule-associated protein tau (p-tau) and extracell

55 linked to neurodegenerative diseases is tau (microtubule-associated protein tau), which can cause fro

56 mulation of hyperphosphorylated forms of the microtubule-associated protein tau.

57 Tau is a microtubule-associated protein that plays a major role i

58 he neuronal-markers; neuronal nuclei (NeuN), microtubule-associated protein-2 (MAP-2) and betaIII-tub

59 ys show that KIF3AC moves processively along microtubules at a rate faster than expected given the mo

60 ule-organizing center (MTOC), nucleating new microtubules at distances far from the nucleus or cell b

61 ese mechanisms, alphaTAT enriches for stable microtubules at the expense of dynamic ones.

62 fluctuations can occur when the kinetochore-microtubule attachment lifetime is long.

63 time on microtubules and enhance kinetochore-microtubule attachment strength.

64 This clustering occurs only after microtubule attachment, and it increases proportionally

65 dded, as well as the restructuring caused by microtubule attachment.

66 us corona layer of the kinetochore following microtubule attachment.

67 Incorrect kinetochore-microtubule attachments during mitosis can lead to chrom

68 d from these regions to regulate kinetochore-microtubule attachments remains unclear.

69 tion of kinetochores extends beyond altering microtubule attachments.

70 Cilia are dynamic microtubule-based organelles present on the surface of m

71 Primary cilia are microtubule-based organelles that play important roles i

72 Centrioles are precisely built microtubule-based structures that assemble centrosomes a

73 proper attachment of kinetochores to spindle microtubules before anaphase onset.

74 Interaction with CMUs did not affect microtubule binding or motility of the FRA1 kinesin but

75 titrations mapped 3-O-S binding sites to the microtubule binding repeat 2 (R2) and proline-rich regio

76 them, Doublecortin-like kinase 1 (DCLK1), a microtubule binding serine threonine kinase, emerged as

77 tributed rigosertib's mechanism of action to microtubule binding.

78 he microtubule rescue factor CLIP-170 in its microtubule-binding domain to increase its rescue-promot

79 We find that the microtubule-binding domains of the Ndc80 complex cluster

80 othesis that PRC1/Ase1 proteins use distinct microtubule-binding domains to control the spindle elong

81 These and other data suggest that the microtubule-binding interface of the human kinetochore b

82 Kinesin is part of the microtubule-binding motor protein superfamily, which ser

83 Thus, although both DNA-binding and microtubule-binding proteins can diffuse on the negative

84 By contrast, the lower net charge on microtubule-binding proteins enables them to diffuse mor

85 ppresses tau hyperphosphorylation within the microtubule-binding region.

86 However, truncated tau species lacking the microtubule-binding repeat (MTBR) domains essential for

87 d LRP1 is mediated by lysine residues in the microtubule-binding repeat region of tau.

88 The microtubule-binding taxanes, docetaxel and cabazitaxel,

89 nesin-5 tails stabilize motor domains in the microtubule-bound state by slowing ATP-binding, resultin

90 cytoskeletal functions, concentrates at the microtubule-branched network interface, whereas APC knoc

91 ik1-Kar3 delays cells in mitosis and impairs microtubule bundle organization and dynamics.

92 In contrast, microtubule bundling in biopsies 2 to 3 days after the f

93 anti-tubulin immunofluorescence to quantify microtubule bundling in interphase cells and aberrant mi

94 The visualization of microtubules by combining optical and electron microscop

95 ruited to the spindle by clathrin stabilizes microtubules by inhibiting the microtubule depolymerase

96 teins from the kinesin-8 family depolymerize microtubules by interacting with their ends in a collect

97 ome segregation demands efficient capture of microtubules by kinetochores and their conversion to sta

98 X. laevis microtubules combine very fast growth and infrequent cat

99 f AIS intracellular membrane, cytosolic, and microtubule compartments.

100 g cells that contain densely packed, dynamic microtubules, comprising the metaphase spindle.

101 etermines microtubule stability and that the microtubule conformation changes gradually in the cap as

102 Siglec-1 localizes mainly on microtubule-containing TNT that are long and carry HIV-1

103 in cardiomyocytes, we sought to test whether microtubules contribute meaningful viscoelastic resistan

104 How cells regulate microtubule cross-linking activity to control the rate a

105 ta preferentially inhibit XCTK2 antiparallel microtubule cross-linking and sliding by decreasing the

106 t tension generated by Cin8 and Kip3 through microtubule cross-linking is essential for signaling eff

107 e-independent enrichment of the antiparallel microtubule crosslinker Prc1 at kinetochores via the Ndc

108 Paradoxically, severases can amplify the microtubule cytoskeleton and not just destroy it.

109 Microtubule cytoskeleton exists in various biochemical f

110 Although it is well known that the microtubule cytoskeleton has a central role in establish

111 , spatiotemporally precise modulation of the microtubule cytoskeleton in living cells, and promise ne

112 The microtubule cytoskeleton plays critically important role

113 The cortical microtubule cytoskeleton thus may provide a platform to

114 ndicated a synergistic decrease of actin and microtubule cytoskeleton-associated proteins in both con

115 differential expression of genes involved in microtubules, cytoskeleton linkages, and motor activity.

116 Both epiboly and microtubule defects were partially restored by pregnenol

117 t 5 um picloram induces immediate changes to microtubule density and later transverse microtubule pat

118 utations disrupting tubulin binding decrease microtubule density at the leading edge of polarized cel

119 ytes exhibit elevated viscosity and reducing microtubule density or detyrosination lowers viscoelasti

120 Reducing either microtubule density or detyrosination reduced myocyte st

121 The microtubule-dependent border cell-oocyte interaction is

122 iting the length and time scales inherent to microtubule-dependent cellular processes.

123 in stabilizes microtubules by inhibiting the microtubule depolymerase MCAK.

124 correction relies on the kinesin-13 MCAK, a microtubule depolymerase whose activity in vitro is supp

125 Moreover, actin and microtubule depolymerization and changing chromatin cond

126 tine, crocin, or colchicine; and 6) leads to microtubule depolymerization in PC3 cells.

127 In myocardial tissue, we found microtubule depolymerization reduced myocardial viscoela

128 lations of rigosertib is responsible for the microtubule-destabilizing activity.

129 Combretastatin A-4 phosphate (CA4P) is a microtubule-disrupting tumour-selective vascular disrupt

130 McTNs are stabilized by an interplay between microtubule-driven protrusion, actomyosin-driven retract

131 res connect centromeric chromatin to spindle microtubules during mitosis.

132 attachments between kinetochores and spindle microtubules during mitosis.

133 vides the major attachment point for spindle microtubules during mitosis.

134 ur method to a simple computational model of microtubule dynamic instability.

135 ted to drive CIN in HGSC, including elevated microtubule dynamics and DNA replication stress that can

136 n two related Xenopus frog species influence microtubule dynamics and spindle length.

137 putational model using a multi-MAP, in vitro microtubule dynamics assay to reconstitute robust plus-e

138 n primary neurons, they enable regulation of microtubule dynamics resolved to subcellular regions wit

139 eveloping arbors have extensive acentrosomal microtubule dynamics, and here, we report an unexpected

140 By slowing down microtubule dynamics, we reveal such a mechanism by show

141 llar granule cells in the context of altered microtubule dynamics, with profound neurodevelopmental d

142 Nevertheless, microtubules, dynein, and kinesin-1 are essential for mi

143 cytoplasmic extract, a new study finds that microtubule end density negatively influences their asse

144 Here, we tracked the microtubule end-binding activity of yeast kinesin-8, Kip

145 decreased the motors' residence time at the microtubule end.

146 P-170's capability to form comets at growing microtubule ends, both phosphomimetic mutations and JNK

147 s Rab6 vesicles to reach freshly polymerized microtubule ends, to which KIF5B binds poorly, likely be

148 al regulator of centrosomal and acentrosomal microtubule formation, yet its structure is not known.

149 his mechanism, benzamides impaired growth of microtubules formed with beta-tubulin harboring Cys239,

150 ted to cortical actin, removes minus-end-out microtubules from axons.

151 In turn, cortical microtubules further stabilize TMK1- and flotillin1-cont

152 plex in a "closed" conformation matching the microtubule geometry.

153 show how the ability of LEM2 to condense on microtubules governs the activation of ESCRTs and coordi

154 hat development of substantial forces during microtubule growth and shortening requires a high activa

155 ed with early endosomal marker Rab5, and new microtubule growth initiated at puncta marked with fz, d

156 g that these enzymes are strong promoters of microtubule growth.

157 On the other hand, DNA and microtubules have different structural properties.

158 the orientation of kinetochore attachment to microtubules in meiosis I.

159 hat KIFC1 is important for organizing axonal microtubules in neurons, a process that depends on the t

160 such as cellular stress, to the integrity of microtubules in order to instruct neuroregeneration.

161 Golgi outposts can nucleate new microtubules in specialized cells with unique cytoarchit

162 letion of Spindly affects polarity of axonal microtubules in vivo and in primary neuronal cultures.

163 leria as a natural model system for studying microtubule-independent cytoskeletal phenotypes.

164 This process is promoted by oocyte-specific, microtubule-independent enrichment of the antiparallel m

165 However, kinetochores initially bind to microtubules indiscriminately, resulting in errors that

166 iclib, a CDK4/6 inhibitor, and paclitaxel, a microtubule inhibitor, synergize with the BET inhibitor

167 sitivity to 31 compounds, including BCL2 and microtubule inhibitors (MTIs).

168 in terms of microtubule number and geometry, microtubule inner proteins, and microtubule linkers.

169 n neurons, a process that depends on the two microtubule-interacting domains [5].

170 We found that HDAC6-microtubule interactions are entirely independent of the

171 s, mediating microtubule zippering or end-on microtubule interactions, depending on their contact ang

172 vide the molecular forces at the kinetochore-microtubule interface and along the spindle to control c

173 ka complex recruitment to the NDC80 complex--microtubule interface.

174 -EM refinement method that divides an imaged microtubule into its constituent protofilaments, enablin

175 fidgetin - are related AAA-ATPases that cut microtubules into shorter filaments.

176 Dynamic instability of microtubules is characterized by stochastically alternat

177 pending on the geometry of forces across the microtubule, kinesin can switch from a fast detaching mo

178 In small spindles, kinetochore microtubules (KMTs) connect directly with the poles, and

179 e substrates to correct improper kinetochore-microtubule (KT-MT) attachments, whereas tension across

180 e the occurrence of CLIP-170 remnants on the microtubule lattice at the rear of comets.

181 170 would rather contribute in preparing the microtubule lattice for future rescues at these predeter

182 On the microtubule lattice, loads also exponentially decreased

183 lands of uncompressed (GMPCPP) dimers in the microtubule lattice.

184 ucleotide state of tubulin dimers within the microtubule lattice.

185 icentrin compactness is lessened and mitotic microtubule length is shortened, as demonstrated by immu

186 nd geometry, microtubule inner proteins, and microtubule linkers.

187 se that modulation of CMU protein levels and microtubule localization by FRA1 provides a mechanism th

188 fferentially affected the protein levels and microtubule localization of CMU1 and CMU2, thus regulati

189 minal coiled-coil CC1 domain is required for microtubule localization, while the C-terminal coiled-co

190 e highlight a specific role for TBCB/Alf1 in microtubule maintenance at low temperatures.

191 ferent tubulins contribute to the control of microtubule mass and therefore set steady-state spindle

192 Centrosomes must resist microtubule-mediated forces for mitotic chromosome segre

193 We have reported that the microtubule-mediated movement of insulin vesicles away f

194 riments demonstrate that TAN1 directly binds microtubules, mediating microtubule zippering or end-on

195 Cytoplasmic dynein is the primary motor for microtubule minus-end-directed transport and is indispen

196 icity, with an interspace of ~41 angstrom of microtubule monomer in cells.

197 tubules and actin filaments, together with a microtubule motor, kinesin-1, and an actin motor, myosin

198 both actomyosin activity and the dynamics of microtubule/motor assemblies in vitro as well as in dive

199 interphase of the eukaryotic cell cycle, the microtubule (MT) cytoskeleton serves as both a supportiv

200 Microtubule (MT) mechanotransduction links diastolic str

201 e 1 (HIV-1) exploits a number of specialized microtubule (MT) plus-end tracking proteins (commonly kn

202 rce in promoting axon growth by facilitating microtubule (MT) polymerization.

203 Intriguingly, Cep63 fused to a microtubule (MT)-binding domain of Cep57 functioned in c

204 Here, we demonstrate that microtubule (MT)-motor-mediated transport dynamics have

205 The evolutionarily conserved microtubule (MT)-severing AAA-ATPase enzyme Katanin is e

206 is central to understanding the behavior of microtubules (MTs) and other cytoskeletal polymers.

207 Microtubules (MTs) mediate mitosis, directional signalin

208 Microtubules (MTs), cylindrical protein polymers compose

209 tracellular cargoes towards the minus end of microtubules (MTs).

210 -1 removes osk/Staufen from the cortex along microtubules, myosin-V anchors osk/Staufen at the cortex

211 tion and/or maintenance depends on an intact microtubule network and a viral tegument protein, pUL51.

212 nd VF coalescence was dependent on an intact microtubule network and actin cytoskeleton.

213 Centrioles organize the microtubule network and mitotic spindle and, as basal bo

214 High glucose-induced remodeling of microtubule network facilitates robust GSIS.

215 in living cells allows optical control over microtubule network integrity and dynamics, cell divisio

216 evealed a reduced interaction of CG with the microtubule network upon a3-subunit knockdown.

217 keratinocytes through pathways that involve microtubule networks and the actin cytoskeleton.

218 lear pore components, endocytic proteins and microtubule networks.

219 The microtubule-nucleating activity of centrosomes is confer

220 How microtubule nucleation and polarity are regulated within

221 hypothesized that Ror may act by regulating microtubule nucleation at baseline and during dendrite r

222 Microtubule nucleation is spatiotemporally regulated in

223 This work provides a blueprint for other microtubule nucleation pathways and helps explain how mi

224 However, the factors that constitute these microtubule nucleation pathways and their mode of action

225 out the structures that promote acentrosomal microtubule nucleation, less is known about the structur

226 eins we biochemically reconstitute branching microtubule nucleation, which is critical for chromosome

227 Each region is distinct in terms of microtubule number and geometry, microtubule inner prote

228 Spindle size depends primarily on microtubule number, which is regulated by a reaction-dif

229 ever, the mechanism that regulates dendritic microtubule organization is still unclear.

230 grity, protein trafficking, and cytoskeletal-microtubule organization, leading to cell cycle arrest,

231 this kinase has a role in regulating overall microtubule organization.

232 s were attributed to aberrant release of the microtubule organizing center (MTOC) linker protein, C-N

233 The centrosome is the microtubule organizing center of human cells and facilit

234 ganelle that can function as an acentrosomal microtubule-organizing center (MTOC), nucleating new mic

235 The switch from centrosomal microtubule-organizing centers (MTOCs) to non-centrosoma

236 ndependent condensates that serve as ectopic microtubule-organizing centres.

237 Cortical microtubules orient the deposition of cellulose by guidi

238 strophe induction with vinblastine prevented microtubule overgrowth and was sufficient to rescue cent

239 to microtubule density and later transverse microtubule patterning in wild-type plants, but does not

240 Thus, exogenous auxin induces transverse microtubule patterning through the TRANSPORT INHIBITOR 1

241 Microtubule treadmilling, in which the microtubule plus end grows while the minus end shrinks,

242 This is followed by recruitment of the microtubule plus end-binding protein EB1/EBP-2 around th

243 domains of the Ndc80 complex cluster at the microtubule plus end.

244 -pixel localization demonstrated that during microtubule plus-end directed transport, both kinesins l

245 Microtubule polarity in axons and dendrites defines the

246 in filament array that specifies anterograde microtubule polymerization and guides these microtubules

247 ion factor Knot regulate transient surges of microtubule polymerization at dendrite tips; they drive

248 , like the dysregulation of genes related to microtubules, presynaptic vesicle alteration, and behavi

249 The mixed oriented microtubules promote dendrite development and facilitate

250 ndings suggest that ringer acts as a hub for microtubule regulators that relays cellular status infor

251 ubulin, and promotes the dynamic assembly of microtubules, remodels the cytoskeleton, and enhances th

252 cell stress, JNK directly phosphorylates the microtubule rescue factor CLIP-170 in its microtubule-bi

253 P-170 remnants, which are potential sites of microtubule rescue, display a shorter lifetime when CLIP

254 d and decreased the nocodazole resistance of microtubules, respectively.

255 MTOC with unique architecture that regulates microtubules, serving vital functions.

256 Microtubule-severing enzymes - katanin, spastin, fidgeti

257 hase A does not stem solely from kinetochore microtubule shortening.

258 (Trc) as a Pavarotti-dependent regulator of microtubule sliding in neurons.

259 These forces scale with microtubule sliding velocity and the number of PRC1 cros

260 esses including actomyosin contractility and microtubule sliding.

261 Our studies suggest a revised microtubule-sliding model, in which kinesin-5 tails stab

262 as +TIPs) to induce the formation of stable microtubules soon after virus entry and promote early st

263 requires the assembly and organization of a microtubule spindle for the proper separation of chromos

264 These azobenzene-based microtubule stabilisers thus enable non-invasive, spatio

265 synthesised photoswitchable paclitaxel-based microtubule stabilisers, whose binding is induced by pho

266 We find that cap length determines microtubule stability and that the microtubule conformat

267 Assays of microtubule stability revealed that both TTL and TTL-E33

268 Importantly, microtubule stabilization suppressed multipolarity by im

269 posed during mitosis to directly recruit the microtubule-stabilizing protein GTSE1 to the spindle.

270 Kinesin-5 tails decrease microtubule-stimulated ATP-hydrolysis by specifically en

271 The ciliary axoneme has a 9 + 2 microtubule structure consisting of nine peripheral doub

272 a-tubulin CTT does not protrude out from the microtubule surface, as is commonly depicted in models,

273 ose proximity, with the GTPase closer to the microtubule surface, whereas the kinase is exposed to th

274 nic interactions with the negatively charged microtubule surface.

275 N) and mto1Delta cells nucleate fewer astral microtubules than normal and have higher levels of Rho1-

276 ow that NM hTau exhibits stronger binding to microtubules than P301L hTau, and is associated with mit

277 that gammaTuRC stably caps the minus ends of microtubules that it nucleates stochastically.

278 and targets condensation of LEM2 to spindle microtubules that traverse the nascent nuclear envelope.

279 eviously described flaring shapes of growing microtubule tips are remarkably consistent under various

280 shing machinery in cells, grow directly from microtubule tips toward the leading edge in growth cones

281 K1 acts together with RHD3 to move the ER on microtubules to generate a fine ER network.

282 rs (TBCs) further sensitizes cells and their microtubules to low temperatures, and we highlight a spe

283 d cortical motor complexes can act on astral microtubules to orient the spindle.

284 st, studying the contribution of tubulin and microtubules to spindle assembly has been limited by the

285 microtubule polymerization and guides these microtubules to subdivide the tip into multiple branches

286 ), and rhodopsin were mislocalized along the microtubules to the IS, cell body, and synaptic region.

287 polymers in axons that are transported along microtubule tracks.

288 Microtubule treadmilling, in which the microtubule plus

289 o determine the conditions leading to robust microtubule treadmilling.

290 The selective insertion of the probe into a microtubule triggers remarkable fluorescence enhancement

291 ckdown in mouse islet beta-cells facilitates microtubule turnover, causing increased basal insulin se

292 ents; and third, we show that by varying the microtubule type, we can change the ratio of backsteps t

293 uced KT, CPC, and SAC proteins, while axonal microtubules were unaffected.

294 erizes as a right-handed double helix around microtubules, which are left-handed.

295 ed to centrosomes by lengthening kinetochore microtubules, which are under tension, suggesting that a

296 led that both TTL and TTL-E331Q depolymerize microtubules, while VASH1 and SVBP depletion reduce dety

297 Earlier studies had shown an association of microtubules with the cleavage furrow, and we used a flu

298 oth plus and minus ends, rather than sliding microtubules within the kinetochore-fiber.

299 y dynamic microtubules (as opposed to stable microtubules) within the distal axon, illuminating a nov

300 TAN1 directly binds microtubules, mediating microtubule zippering or end-on microtubule interactions