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1 A contacts within the -20 to -40 region of a middle promoter.
2 egion 4 and with a DNA element present in T4 middle promoters.
3 cherichia coli transcription apparatus to T4 middle promoters.
4 the T4 MotA protein, for activation of phage middle promoters.
5 sor and Ndt80 activator for occupancy at key middle promoters.
6 polymerase are needed to transcribe from T4 middle promoters.
7 nto middle genes and by the activation of T4 middle promoters.
8 rs and coactivating transcription from phage middle promoters.
9 ential co-activator of transcription from T4 middle promoters.
10 MotA alone binds to the -30 region of T4 middle promoters, a region that contains the MotA box co
11 how that AsiA also plays a direct role in T4 middle promoter activation by contacting the MotA activa
13 findings, we propose a revised model for T4 middle promoter activation, with AsiA organizing the act
16 p2 protein, Drc likely binds locally unwound middle promoters and recruits the phage RNA polymerase.
17 onsistent with data on how MotA functions at middle promoters, and provides an explanation for why Mo
19 n, the MotA transcription factor binds to T4 middle promoters at a -30 mot box consensus sequence to
20 pressor and Ndt80 activator proteins control middle promoters by binding to overlapping DNA elements.
27 that the Sum1 repressor can be removed from middle promoters in meiotic cells independent of Ndt80 e
29 ole as co-activator, AsiA directs RNAP to T4 middle promoters in the presence of the T4-encoded activ
32 It has been proposed that the induction of middle promoters is controlled by competition between Nd
33 ription by unmodified polymerase from the T4 middle promoter P uvsx is independent of the specific se
35 s, Mot21 and MotA bind DNA containing the T4 middle promoter P(uvsX) similarly, and the proteins yiel
42 subjected to a comparative analysis: the T4 middle promoter PrIIB2 opens and closes thermoreversibly
43 r the binding of polymerase/MotA/AsiA to the middle promoter PuvsXis inhibited by premethylation of g
44 anscriptional activation of bacteriophage T4 middle promoters requires sigma70-containing Escherichia
45 and the phage genome appears to lack typical middle promoter sequences, KVP40 AsiA activates transcri
47 r activate transcription in vitro using a T4 middle promoter that is active with RNA polymerase alone
48 romoters and promotes transcription at phage middle promoters through its interaction with the primar
50 s involved in N4 RNAPII transcription and of middle promoters, we propose a model for N4 RNAPII promo
51 s as a co-activator of transcription from T4 middle promoters, which retain the canonical sigma70 -10