ライフサイエンスコーパス: minimal medium

1 soluble cruzain in high yields (>30 mg/L in minimal medium).

2 in a population of log phase cells grown in minimal medium.

3 attTn7 sites can be obtained by selection on minimal medium.

4 119 mutants were unable to grow on glycerol minimal medium.

5 erine during growth on glycerol and d-serine minimal medium.

6 ation and repressed when Fe(2+) was added to minimal medium.

7 eened in this study on glycerol-supplemented minimal medium.

8 Growth of S40 was severely impaired in minimal medium.

9 ins during chronic low-level AZT exposure in minimal medium.

10 t cannot use thymine for growth on a glucose minimal medium.

11 ant proteins in E. coli grown aerobically in minimal medium.

12 growing in conditioned complex medium or in minimal medium.

13 ion for Asp-tRNA(Asn) formation by growth in minimal medium.

14 h in the absence of thymine on LB but not on minimal medium.

15 mplex (Luria-Bertani [LB]) medium but not on minimal medium.

16 of cations or by a shift from rich to acidic minimal medium.

17 t not enough to support growth of E. coli in minimal medium.

18 orted the growth of the pfl mutant in xylose minimal medium.

19 RNA is increased at a low temperature and in minimal medium.

20 ent at the level of transcription in glucose minimal medium.

21 ficant lag in growth when diluted into fresh minimal medium.

22 tB during stationary phase in both broth and minimal medium.

23 hen grown in rich medium or in vir induction minimal medium.

24 651 mutant is viable on LB medium but not on minimal medium.

25 expressed in both ordinary and perdeuterated minimal medium.

26 the shrimp waste disposal site using chitin minimal medium.

27 ng 2,000 generations of evolution in glucose minimal medium.

28 tive to their Rbs(+) counterparts in glucose minimal medium.

29 9 yeast was analyzed for growth and color on minimal medium.

30 s when grown on rich (Luria-Bertani) than on minimal medium.

31 they were provided as sole carbon sources in minimal medium.

32 ained constant and high throughout growth in minimal medium.

33 growth in rich medium and host cells but not minimal medium.

34 domain expresses at high yield (20 mg/l) in minimal medium.

35 um, such as Luria-Bertani medium, versus the minimal medium.

36 erichia coli strain SAB11 in iron-restricted minimal medium.

37 uantitative growth defects in either rich or minimal medium.

38 more slowly than wild-type cells in glucose minimal medium.

39 ties when cultured in rich medium or glucose-minimal medium.

40 th defect of the E. coli sodA sodB strain in minimal medium.

41 the enzyme, as judged by growth on succinate minimal medium.

42 tein is present by 2 h after suspension in N minimal medium.

43 ures in secY40 cells grown in either rich or minimal medium.

44 tein export defects in secY40 cells grown in minimal medium.

45 imal medium relative to its level in glucose minimal medium.

46 g growth in Luria-Bertani medium and glucose minimal medium.

47 rosmotic medium, and one failed to grow in a minimal medium.

48 gh wild type (arg-13+) is not derepressed in minimal medium.

49 n initiation defects that are exacerbated in minimal medium.

50 splay increased DCS resistance when grown in minimal medium.

51 sensitivity when strains are grown in a non-minimal medium.

52 urthermore, exhibit slow growth in Edinburgh minimal medium.

53 nce, with an upper limit of 225 mosmol/kg in minimal medium.

54 wild-type Escherichia coli growth on glucose minimal medium.

55 hways that are active in LB broth but not in minimal medium.

56 ellular pH of 7 than after growth at pH 5 in minimal medium.

57 d minimal medium, to a completely deuterated minimal medium.

58 well as by submerged mycelia grown in liquid minimal medium.

59 the chvD mutant was reduced in rich, but not minimal, medium.

60 ift-down from glucose amino acids to glucose minimal medium; (2) carbon source starvation after a "gl

61 In minimal medium, 30-40 mg of M7FP were obtained per liter

62 atis LR222 on iron-limiting (0.1 micro M Fe) minimal medium agar fluoresce under UV light due to the

63 Z expression was also observed in unbuffered minimal medium and appeared to be exerted posttranslatio

64 copy of fhs restored its ability to grow in minimal medium and at acidic pH as well as to produce mu

65 4-kilobase (kb) transcript is synthesized in minimal medium and both a 1.4- and 1.7-kb transcript are

66 Mutations in arg-13 result in slow growth in minimal medium and can suppress mutations in carbamyl ph

67 te secretome of A. nosocomialis strain M2 in minimal medium and demonstrate that pathogenic Acinetoba

68 ent growth defect in either nutrient-rich or minimal medium and no measurable virulence phenotype.

69 tions: high salt, sorbitol, galactose, pH 8, minimal medium and nystatin treatment.

70 educed cell size of PssB728a when grown in a minimal medium and on plant surfaces, while most ppGpp(0

71 ion in P. multocida in response to growth in minimal medium and provide a strong foundation to invest

72 ructose minimal medium but that in plant oil minimal medium and rich medium, phaB3 seems to be unexpr

73 at GadW represses the decarboxylase genes in minimal medium and that growth under acidic conditions l

74 ever, Deltaptc4 cells grow slowly in defined minimal medium and undergo premature growth arrest in re

75 e when this strain in cultivated in low-iron minimal medium, and a comparison of the growth character

76 ll appears to be optimal for rapid growth in minimal medium, and the gltBDF control region is designe

77 693 relA spoT double mutant for growth on M9 minimal medium, and the transformed cells produced rel(B

78 se protein, required glutamate for growth in minimal medium, and was unable to sporulate efficiently

79 features of E. coli cells growing on glucose minimal medium appeared to be the formation and excretio

80 Acid induction during log-phase growth in minimal medium appears to occur through multiple pathway

81 l (plasmolysis) titrations of cells grown in minimal medium at 0.03 Osm reduce cytoplasmic and cell w

82 ild-type and MG1655(rluA-) either in rich or minimal medium at 24, 37, or 42 degrees C, but when both

83 lpxP triple mutant, likewise grows slowly on minimal medium at all temperatures but not on nutrient b

84 and lpxL lpxM double mutants grow slowly on minimal medium at all temperatures, they do not grow on

85 rich medium at high induction levels and in minimal medium at low induction levels.

86 10b resulted in poor growth of the mutant in minimal medium at near the optimal growth temperature bu

87 Using minimal-medium batch and chemostat cultures, we comprehe

88 on 0.3% agar motility plates and in rich and minimal medium broth.

89 onditional: it can be maintained in cells in minimal medium but cannot be established in cells growin

90 ant had decreased growth in glucose-limiting minimal medium but grew normally when excess glucose was

91 cell envelope when the organism is grown in minimal medium but is secreted during growth in nutrient

92 /E57G and K31N/A50G/L218R) allowed growth on minimal medium but resulted in thiamine auxotrophy when

93 3 contribute to PHB biosynthesis in fructose minimal medium but that in plant oil minimal medium and

94 t extract-peptone complex medium and glucose minimal medium but was incapable of growth on glycerol.

95 face when E. coli O157:H7 was cultured in M9 minimal medium but were expressed from only a proportion

96 utes to E. coli biofilm formation in glucose-minimal medium, but not in Luria-Bertani broth.

97 from cultures grown in an hrp gene-inducing minimal medium by immunodetection with a DspE-specific a

98 rongly induced in fructose- or glucose-based minimal medium by L-phenylalanine.

99 abolic and regulatory programs of E. coli in minimal medium by reaction with homocysteine and cystein

100 In minimal medium, CFT073 dsdX can grow on d-serine as a so

101 ly 3-fold increase was seen during growth in minimal medium compared with rich medium.

102 e mid-logarithmic phase on acidified glucose minimal medium, conditions that induce glutamate-depende

103 asite gene restores growth of this mutant on minimal medium, confirming that the protein has IMPDH ac

104 on caused a growth defect at 45 degrees C in minimal medium containing 0.2 M NaCl that was similar to

105 In N minimal medium containing 10 mM Mg2+, all strains grew a

106 iphosphates isolated from E. coli grown in a minimal medium containing 12C, 14N-enriched glucose and

107 xS isogenic mutant of PAO1 did not grow in a minimal medium containing 2-ketogluconate as the sole ca

108 Cells produced (14)CO(2) from minimal medium containing [(14)C]adenine, which implies

109 Mxr1p is cytosolic in cells cultured in minimal medium containing a yeast nitrogen base, ammoniu

110 four- to sevenfold when cells were grown in minimal medium containing alanine or leucine.

111 , and JM101 allowed these strains to grow on minimal medium containing d-glucosaminate as the sole ca

112 e altered if the enzyme was overexpressed in minimal medium containing Fe and Mn, and surprisingly, a

113 in, OG1RF_12399-12402 are unable to grow in minimal medium containing gluconate.

114 During rapid growth in minimal medium containing glucose and amino acids, CodY

115 and exhibited a bald phenotype when grown on minimal medium containing glucose as carbon source.

116 ae cells was explored in batch cultures on a minimal medium containing glucose as the sole carbon sou

117 rtant during exponential growth in acidified minimal medium containing glucose but were unnecessary f

118 tant E. coli by growing transformed cells on minimal medium containing glucuronic acid.

119 ch medium, AldA was required for growth on a minimal medium containing L-alanine as the major source

120 ressors of the cbr mutant were selected from minimal medium containing l-arginine as the sole carbon

121 trains lacking Rv1422 have growth defects in minimal medium containing limiting amounts of several di

122 the mig-14 promoter is induced by growth in minimal medium containing low magnesium or acidic pH, co

123 ects in the DeltapylT strain were evident in minimal medium containing methanol.

124 ved in comparison with that of the mutant in minimal medium containing mucin but not in the absence o

125 the fadD5 mutant was diminished in growth in minimal medium containing mycolic acid but not other lon

126 fragment conferred the capacity to grow on a minimal medium containing only 10 mM K+.

127 es measured in two media are necessary: in a minimal medium containing only derivatized metabolites a

128 mutant in this fungus that fails to grow in minimal medium containing polyamines as the sole nitroge

129 2-heptyl-3-hydroxy-4(1H)-quinolone (PQS), a minimal medium containing PQS as the sole carbon source

130 n in lacR mutants was seen in cells grown in minimal medium containing succinate and raffinose and gr

131 B. subtilis is unable to grow in minimal medium containing the iron chelator EDDHA unless

132 e-overproducing derivatives were selected on minimal medium containing the toxic proline analog 3,4-d

133 the iscS(-) strain is switched from rich to minimal medium containing thiamin and nicotinate, growth

134 sion and growth of P. pastoris cultured in a minimal medium containing yeast nitrogen base and methan

135 lx2) was overexpressed in rich medium and in minimal medium containing zinc, iron, or cobalt, and the

136 shed cells, inoculated at low density into a minimal medium, could not grow in its absence.

137 produced wild-type levels of cephamycin C in minimal medium culture conditions supplemented with lysi

138 s of the fhs mutant grown anaerobically in a minimal medium demonstrated that the mutant had an absol

139 Anaerobic induction in minimal medium depends strongly on FNR but is also affec

140 i host strain suspended in 5TFI-supplemented minimal medium depleted of isoleucine.

141 fied effector proteins secreted into defined minimal medium designed to induce expression of the SPI-

142 late ammonia as a sole source of nitrogen in minimal medium devoid of organic nitrogen sources.

143 Growth in GM medium plus cAMP or glycerol minimal medium did not result in a significant increase

144 ld type, was outcompeted by the wild type in minimal medium, displayed reduced swimming and swarming

145 lexigens is able to grow up to 3 m NaCl in a minimal medium due to the de novo synthesis of ectoines.

146 DH10B requires leucine for growth on minimal medium due to the deletion of leuLABCD and harbo

147 acetylglutaminylglutamine amide (NAGGN) in a minimal medium, due possibly to NAGGN synthesis depletin

148 demonstrate that the widely used Essential 8 minimal medium (E8) captures hPSCs at a naive-to-primed

149 in cells growing in steady state in glucose minimal medium, either in the presence or absence of leu

150 o effect on citB expression during growth in minimal medium even when combined with ccpC and abrB mut

151 phenazine biosynthetic gene transcription in minimal medium even when quorum-sensing signals were at

152 cid reported previously unexpectedly grew on minimal medium following transductional introduction of

153 of Escherichia coli propagated in a glucose minimal medium for 20,000 generations.

154 uction in cell mass by shifting to succinate minimal medium for inductions of the S105 gene.

155 ndant and/or share similar functions: (i) on minimal medium GreA overproduction suppresses the growth

156 mance of both models on predicting empirical minimal medium growth data/essential gene listings.

157 sed by reducing secondary initiation through minimal medium growth.

158 rage (19-fold) in continuous-flow cells with minimal medium (growth rate not altered and biofilm rest

159 imulates gdhA transcription during growth in minimal medium has been proposed to be the K. pneumoniae

160 zyme purified from the same E. coli grown in minimal medium has no color.

161 owth of the A1A2-deficient (A1A2-) mutant in minimal medium, implying that lipoate-dependent metaboli

162 complete medium is greater than that in the minimal medium in at least one peak, then unchanged D is

163 This mutant cannot grow in minimal medium in the absence of riboflavin supplementat

164 d simply upon switch from a rich medium to a minimal medium in the complete absence of nitrogen starv

165 guishable from the wild type when grown with minimal medium in the light and contained wild-type leve

166 argR mutant strain PAO501 grown in glutamate minimal medium in the presence and absence of arginine.

167 arent strain, the mutant failed to grow on a minimal medium in which 2-ketogluconate was the sole car

168 of wild-type M. catarrhalis was observed in minimal medium in which arginine was present only in met

169 1.15 +/- 0.05 mg/L and 4.6 +/- 0.9 mg/L in a minimal medium in which either 1 mM tyrosine or 1 mM tyr

170 19 conferred its greatest advantage in basic minimal medium in which either glucose or Casamino Acids

171 Unexpectedly, however, when grown in MOPS minimal medium, in mixed cultures, more hmp mutant cells

172 P1a or LpoA exhibited growth deficiencies in minimal medium, in the presence of deoxycholate and bile

173 exhibited several growth defects in glucose minimal medium, including reduced rates of growth and gr

174 nd citZ was derepressed in glucose-glutamine minimal medium, indicating that CcpC is a negative regul

175 ght major extracellular proteins (EXPs) in a minimal medium inducing hrp genes.

176 iments, wild-type yeast incubated in expired minimal medium instead of water lost viability quickly;

177 by chelators or a shift from rich to acidic minimal medium is largely dependent on functional EnvZ.

178 LpxC purified from Escherichia coli grown in minimal medium is mainly Fe(II).

179 f Chlamydomonas reinhardtii does not grow on minimal medium, is high light-sensitive under photoheter

180 When PA14 is grown on minimal medium, killing occurs over the course of severa

181 For cells grown in minimal medium, L12 acetylation and processing is altere

182 evisiae, is essential for growth in low iron minimal medium lacking citric acid.

183 e synthetases (DeltarelAPQ) does not grow in minimal medium lacking the branched-chain amino acids (B

184 In minimal medium, loss of PBPs 2c and 4 resulted in a slig

185 For cells in a minimal medium, Lrp levels were consistently lowest duri

186 smolalities (1.02-2.17 Osm) in MOPS-buffered minimal medium (MBM) containing 1 mM betaine (MBM+GB).

187 ni (LB) (0.07 muW/cm(2)) or a defined growth minimal medium (MM) (0.02 muW/cm(2)).

188 Genes with increased expression in minimal medium (n = 230) included those encoding amino a

189 llular fitness, measured as growth rate in a minimal medium, of UVR-exposed lineages of both B86-17 a

190 ly the outer membrane porin OmpU, whereas in minimal medium, OmpT is the dominant porin.

191 ually and other genes required for growth in minimal medium or for sporulation.

192 ive analysis of 12 hvKP strains in iron-poor minimal medium or human ascites fluid showed a significa

193 of the mutant to grow on glucose-containing minimal medium or increase its growth rate in the presen

194 argely suppressed when strains were grown on minimal medium or sucrose-free R2YE, where division site

195 nsate for the loss of both FtsL and DivIC on minimal medium or sucrose-free R2YE.

196 e amounts of 2-methylhopanoids when grown in minimal medium or with added methionine, the presumed bi

197 Remarkably, Escherichia coli grows in minimal medium over a wide range of external osmolalitie

198 We also show that minimal medium, pH 4.5, induces SPI-2 gene expression in

199 In minimal medium, phtA mutants do not replicate; in rich b

200 When grown in minimal medium plus acetate (McA), all ehb mutants had s

201 Yeast grown in minimal medium plus lysine show significant reductions i

202 H. capsulatum mycelia in chemically defined minimal medium produced pigmented conidia.

203 with a six-histidine tag using an optimized minimal-medium protocol for subsequent purification.

204 Surprisingly, growth in minimal medium reduced the ability of seqA+ strains to f

205 MP (cAMP) and was elevated also in succinate minimal medium relative to its level in glucose minimal

206 A minimal medium required by the model to generate require

207 mol/OD460 in glucose amino acids and glucose minimal medium, respectively, to about 100 pmol ppGpp/OD

208 However, the addition of Casamino Acids to minimal medium results in a dose-dependent decrease in h

209 growth and fast swimming, while evolution in minimal medium results in fast growth and slow swimming.

210 Batch culture experiments on minimal medium revealed that LSG184 grows well on a vari

211 pe2Delta mutant in a standard polyamine-free minimal medium (SDC) leads to marked increases in cellul

212 ated for thousands of generations in glucose minimal medium show heritable increases in both cell siz

213 The cells grown on minimal medium showed significantly elevated expression

214 utilization (hut) enzymes in cells grown in minimal medium showed that neither the ptsH1 nor the crh

215 ll mutation in nac displayed growth rates on minimal medium similar to the wild type.

216 of a (p)ppGpp(0) Escherichia coli strain in minimal medium, SMG and on medium containing 3-amino-1,2

217 The adaptation to minimal medium strongly up-regulated amino acid synthesi

218 The use of a minimal medium sufficient for hESC growth is expected to

219 tive in motility, luminescence, or growth in minimal medium, suggesting that it lacks an essential, p

220 grew well in rich medium but did not grow in minimal medium supplemented by arginine and nucleosides.

221 ged exponential growth was performed with M9 minimal medium supplemented with 2 g of alpha-ketoglutar

222 restored growth of an rcnA mutant in glucose minimal medium supplemented with 4 microM Ni(II), thus c

223 ient labeling of riboses following growth in minimal medium supplemented with [2-(13)C]acetate.

224 Fpg was also expressed in minimal medium supplemented with cobalt nitrate to yield

225 ne in M. smegmatis could be achieved only in minimal medium supplemented with D-glutamate, demonstrat

226 These mutants displayed attenuated growth in minimal medium supplemented with glucose as the sole car

227 presses Vibrio cholerae biofilm formation in minimal medium supplemented with glucose or pyruvate.

228 5 inner colony mutants had reduced growth on minimal medium supplemented with glucose-6-phosphate.

229 get found to be regulated by GcvB in glucose minimal medium supplemented with glycine.

230 sstT-lacZ in Luria-Bertani broth and glucose minimal medium supplemented with glycine.

231 about threefold when the cells were grown in minimal medium supplemented with leucine.

232 An isogenic zwf mutant was unable to grow on minimal medium supplemented with mannitol.

233 eleterious to the growth of a prp+ strain on minimal medium supplemented with propionate as a carbon

234 bacterial growth and PTS gene expression in minimal medium supplemented with selected carbohydrates.

235 ressing heterologous VcChiP could grow on M9 minimal medium supplemented with small chitooligosacchar

236 'leaky' growth phenotype on aerobic glucose minimal medium supplemented with succinate (which bypass

237 tants were unable to grow on 1,2-propanediol minimal medium supplemented with vitamin B(12) but were

238 in dilute rich soft-agar swarm medium or in minimal-medium swarm plates containing any 1 of 60 chemo

239 hich was consistently higher in hrp-inducing minimal medium than in nutrient-rich Luria-Bertani broth

240 expressed at significantly higher levels in minimal medium than in rich medium, and rpoS expression

241 re 3- to 4-fold higher in cells growing in a minimal medium than those in a rich medium.

242 ; the mutant showed a reduced growth rate in minimal medium that could be reversed by the addition of

243 s, and (ii) a selective advantage in glucose minimal medium that drove these mutants to fixation.

244 istoplasma genome and confirmed by growth in minimal medium that Histoplasma yeasts can synthesize al

245 When grown in minimal medium, the majority (approximately 89%) of the

246 omplete and homogeneous in all cases, but in minimal medium, the periplasm is evidently thicker at th

247 ubations were carried out in iron-containing minimal medium, the products formed colored chelates.

248 During growth in minimal medium, the ptsH1 mutation, which prevents seryl

249 nstrate that, during R. capsulatus growth on minimal medium, the requirement for CycH in c-type cytoc

250 grees C and FH12 lacking the plasmid grew on minimal medium, thereby establishing that idi is a nones

251 e enzyme were overexpressed in perdeuterated minimal medium to allow detection and assignment of prot

252 ic growth, growth on paraquat, and growth on minimal medium to an Escherichia coli (Ec) mutant defici

253 is drug after nutritional shiftup, e.g. from minimal medium to Luria broth.

254 In E. coli cells grown in minimal medium to mid-log phase, the ratio of free to DN

255 a protonated full medium, over a protonated minimal medium, to a completely deuterated minimal mediu

256 In defined minimal medium, transcription of cspA, -B, -G, and -I wa

257 o model realistic cells growing in a glucose minimal medium under aerobic conditions.

258 tyl phosphate, also failed to grow in xylose minimal medium under anaerobic conditions, confirming th

259 rmed optimally when bacteria were grown in a minimal medium under anaerobic conditions.

260 inhibits cell growth of Escherichia coli in minimal medium under anaerobic growth conditions and tha

261 ts were generated and screened for growth on minimal medium under high CO(2) conditions (5% CO(2) in

262 the class II mutants were unable to grow on minimal medium unless a complex mixture of amino acids w

263 es the aconitase prevented growth in glucose minimal medium unless heme or succinate was added to the

264 prevented the growth of Escherichia coli in minimal medium unless the medium was supplemented with a

265 hore membranes obtained using cells grown in minimal medium was decreased to approximately 50%.

266 Growth of this mutant in minimal medium was inhibited by the aryl acids, but the

267 Acid resistance of the fur mutant in minimal medium was restored by addition of glutamate dur

268 In minimal medium, we detected transcripts for approximatel

269 re evolved for 50,000 generations in glucose minimal medium, we observed modest changes in relative f

270 ation is predominant when cells are grown in minimal medium, we propose that these modifications form

271 that allow growth of lipB strains on glucose minimal medium; we determined that suppression was cause

272 cspE mRNA levels in defined minimal medium were drastically higher than mRNA for the

273 B levels in Bacillus subtilis cells grown in minimal medium were increased approximately 7-fold by GB

274 on the growth conditions; lysogens grown in minimal medium were nearly stable but switched at high r

275 In minimal medium where phenazine production is very low, i

276 ter is knocked out showed impaired growth in minimal medium where the only source of arginine came fr

277 When parallel studies were conducted in minimal medium, where the mutD5 strain is defective in e

278 However, the wild type self-aggregates in minimal medium, whereas the quorum-sensing mutant does n

279 Bacillus tequilensis cells grown in a minimal medium with (15)NH4Cl as the nitrogen source wer

280 The supplementation of a minimal medium with a mixture of asparagine, arginine, g

281 A gdhA mutant was growth deficient in minimal medium with citrate as the sole carbon source, a

282 CFT073 cycA can grow on minimal medium with d-serine as a sole carbon source.

283 s to restore growth of a Deltamdh2 strain on minimal medium with ethanol or acetate as the carbon sou

284 E. coli cells were grown in minimal medium with fixed final concentrations of carbon

285 nstrable PckA enzyme activity and grew on AB minimal medium with glucose but did not grow on the same

286 Such mutants grew well in minimal medium with glutamate as the sole nitrogen sourc

287 e of Escherichia coli strain MG1655 grown in minimal medium with glycerol as the carbon source.

288 not only by serum but also during growth in minimal medium with glycerol as the sole carbon source a

289 of H. capsulatum yeast in chemically defined minimal medium with L-3,4-dihydroxyphenylalanine (DOPA)

290 The ald mutants grew poorly in minimal medium with L-alanine as the sole nitrogen sourc

291 was also eliminated upon supplementation of minimal medium with serine or glycine for solid medium o

292 was also shown to restore growth to EV78 in minimal medium with sialic acid as the sole carbon sourc

293 ring a five-week time course the MC grown in minimal medium with sugarcane bagasse (SCB) as a sole ca

294 e MSDH activity and the ability to grow in a minimal medium with valine or isobutyrate as the sole ca

295 mgtC+ mgtB strain was also able to grow in N minimal medium without added Mg2+ but only after a 24-h

296 nd mgtC+ mgtB+ strains exhibited growth in N minimal medium without added Mg2+ with a 1- to 2-h lag p

297 lecting for growth of cells lacking ppGpp on minimal medium without amino acids.

298 to grow under anaerobic conditions in xylose minimal medium without any negative effect on their surv

299 we achieve 12.63 g L(-1) shikimate titer in minimal medium without inducer.

300 allowing growth on 300 microm 5MT-containing minimal medium without tryptophan, and cell extracts con