ライフサイエンスコーパス: minus-end-directed

1 s KlpA without the tail is nonprocessive and minus end-directed.

2 o which the motor domain binds, KlpA becomes minus end-directed.

3 s-end directed molecular motor, while ncd is minus-end directed.

4 Myosin VI (Myo6) is a minus end-directed actin-based motor found in neurons th

5 a yeast two-hybrid screen, we identified the minus-end-directed actin-based motor, myosin VI, as an S

6 irected activity of cytoplasmic dynein and a minus end-directed activity associated with NuMA during

7 end-directed activity of Eg5 antagonizes the minus end-directed activity of cytoplasmic dynein and a

8 dhc-1 and unc-116, components of the dynein minus-end-directed and kinesin-1 plus-end-directed micro

9 makes the dynamics of Ncd non-processive and minus-end directed as opposed to kinesin-1.

10 s, is specifically altered; net transport is minus-end directed at developmental stages when it is no

11 Nonclaret disjunctional (Ncd) is a minus end-directed, C-terminal motor protein that is req

12 dynein is a molecular motor responsible for minus-end-directed cargo transport along microtubules (M

13 Cytoplasmic dynein is the major microtubule minus-end-directed cellular motor.

14 Motor proteins of the minus end-directed cytoplasmic dynein and plus end-direc

15 Live-cell imaging reveals minus end-directed dynein-dynactin motility along microt

16 us ends, suggesting that NUDF may facilitate minus-end-directed dynein movement.

17 pronucleus, while simultaneously generating minus-end directed flows along MTs that are similar to t

18 -side poles into initial contact, but active minus end-directed force generation will be needed to ac

19 combination of two aggregate properties, Net Minus-end-directed Force and microtubule Cross-linking O

20 Kar3Cik1 then uses its minus-end-directed force to depolymerize microtubules fr

21 Kinesin-14 motors generate microtubule minus-end-directed force used in mitosis and meiosis.

22 or inhibitors of autophagy or by modulating minus-end-directed (HDAC6 shRNA) or plus-end-directed (R

23 LIS1) in the interaction of end tracking and minus end-directed human dynein complexes with these sit

24 GTRC encodes a processive minus-end-directed KCHb kinesin, and its N-terminal, C-t

25 are the first to localize the activity of a minus end-directed kinesin at the plus ends of microtubu

26 3 protein from Saccharomyces cerevisiae is a minus end-directed kinesin family member that is involve

27 This sliding was mediated by the minus end-directed kinesin motor Klp2, which helped micr

28 We find that Kar3p, a minus end-directed kinesin motor protein, is required, w

29 ns (KCHs) belong to a distinct branch of the minus end-directed kinesin subfamily.

30 nusual structure, Trkin encodes a functional minus end-directed kinesin that specifically colocalizes

31 uclear positioning via inhibition of Klp2, a minus end-directed kinesin-14.

32 r with a depolymerase (Kip3, class Kin-8) or minus-end-directed kinesin (Kar3, class Kin-14), can sup

33 Here we report that depletion of KIFC1, a minus-end-directed kinesin called HSET in humans, causes

34 nteraction between the Kar3 motor protein, a minus-end-directed kinesin from yeast, and its associate

35 led-coil domains from a plus-end-directed or minus-end-directed kinesin fused to streptavidin.

36 KIFC3, a minus-end-directed kinesin, is recruited to plus-ends of

37 tween yellow fluorescent protein (YFP) and a minus-end-directed Kinesin-14 (C-terminal family) from A

38 st, merotely is increased by deletion of the minus-end-directed kinesin-14 (Klp2) or overexpression o

39 We found that perturbation of the minus-end-directed Kinesin-14 HSET increased the frequen

40 Further, our results show that the minus-end-directed kinesin-14 HSET/KIFC1 suppresses tubu

41 In this work, we explored the role of minus-end-directed Kinesin-14 motor forces in controllin

42 the role of tetrameric Kif25, a microtubule minus-end-directed kinesin-14 motor, in preventing prema

43 analysed the motility of the six members of minus-end-directed kinesin-14 motors in the moss Physcom

44 Kar3, a Saccharomyces cerevisiae microtubule minus-end-directed kinesin-14, dimerizes with either Vik

45 catalytic core, a signature neck peptide of minus end-directed kinesins, and a unique calponin homol

46 a C-terminal motor domain contains all known minus end-directed kinesins.

47 Myosin VI, an actin-dependent, minus-end directed mechanoenzyme, has been implicated in

48 y, indicating that dynein is the predominant minus end-directed membrane motor in Xenopus egg extract

49 related well with the eightfold reduction in minus end-directed membrane transport observed in metaph

50 The minus end-directed microtubule motor cytoplasmic dynein

51 The minus end-directed microtubule motor cytoplasmic dynein

52 Cytoplasmic dynein is the major minus end-directed microtubule motor in eukaryotes.

53 Genetic studies imply that the minus end-directed microtubule motor kinesin-like calmod

54 , using proteomic profiling, we identify the minus end-directed microtubule motor protein HSET as a d

55 e calmodulin (CaM) binding protein (KCBP), a minus end-directed microtubule motor protein unique to p

56 Cytoplasmic dynein 1 (dynein) is a minus end-directed microtubule motor protein with many c

57 To test the hypothesis that the minus end-directed microtubule motor protein, cytoplasmi

58 Non-claret disjunctional protein (Ncd) is a minus end-directed microtubule motor required for normal

59 Cytoplasmic dynein is a minus end-directed microtubule motor that performs disti

60 Cytoplasmic dynein is a minus end-directed microtubule motor that transports int

61 Dynein is a minus end-directed microtubule motor that transports num

62 Cytoplasmic dynein is a multisubunit, minus end-directed microtubule motor that uses dynactin

63 Cytoplasmic dynein is an enormous minus end-directed microtubule motor.

64 esin-14s are commonly known as nonprocessive minus end-directed microtubule motors that function main

65 Therefore, myoJ cooperates with plus and minus end-directed microtubule motors to drive the norma

66 abE engage myosin-5 as well as plus end- and minus end-directed microtubule motors, providing an expe

67 hosphate (GTP)-Rab7 effectors that instigate minus end-directed microtubule transport.

68 Targeting of the minus-end directed microtubule motor cytoplasmic dynein

69 ) component of dynactin, an activator of the minus-end directed microtubule motor dynein; an associat

70 osition depends on a balance of plus-end and minus-end directed microtubule motor function.

71 Kar3 is a minus-end directed microtubule motor involved in meiosis

72 The minus-end directed microtubule motor protein cytoplasmic

73 1, the heavy chain of the cytoplasmic dynein minus-end directed microtubule motor, in a genetic scree

74 Cytoplasmic dynein, a major minus-end directed microtubule motor, plays essential ro

75 organelle in possession of both plus-end and minus-end directed microtubule motors and a myosin; coor

76 Cytoplasmic dynein is the major minus-end directed microtubule-based motor in eukaryotic

77 Cytoplasmic dynein is a minus-end directed microtubule-based motor.

78 Cytoplasmic dynein is the predominant minus-end-directed microtubule (MT) motor in most eukary

79 ubules from the plus end and promotes robust minus-end-directed microtubule gliding.

80 Ncd is a minus-end-directed microtubule motor and a member of the

81 ks to facilitate the transport driven by the minus-end-directed microtubule motor cytoplasmic dynein.

82 Dynactin is a required cofactor for the minus-end-directed microtubule motor cytoplasmic dynein.

83 HIP) (FHF) complex, which interacts with the minus-end-directed microtubule motor dynein and its acti

84 s cytoplasmic dynein is primarily known as a minus-end-directed microtubule motor for organelle trans

85 Dynein is a minus-end-directed microtubule motor important for mitot

86 regulator of cytoplasmic dynein-1, the major minus-end-directed microtubule motor in many eukaryotes.

87 Cytoplasmic dynein is the primary minus-end-directed microtubule motor protein in animal c

88 arrays in dividing cells suggests that this minus-end-directed microtubule motor protein is likely t

89 Dynein is the primary minus-end-directed microtubule motor protein.

90 Kar3 is a minus-end-directed microtubule motor that is implicated

91 Cytoplasmic dynein is a minus-end-directed microtubule motor that participates i

92 Cytoplasmic dynein is a multisubunit minus-end-directed microtubule motor that serves multipl

93 Cytoplasmic dynein is a minus-end-directed microtubule motor whose mechanism of

94 ment (cVAC) show a clear requirement for the minus-end-directed microtubule motor, dynein, for virus

95 Cytoplasmic dynein, a minus-end-directed microtubule motor, has been implicate

96 Cytoplasmic dynein, a minus-end-directed microtubule motor, has been implicate

97 behavior of cytoplasmic dynein, the primary minus-end-directed microtubule motor.

98 oteins (KLPs) are a large family of plus- or minus-end-directed microtubule motors important in intra

99 nd by extension those of intact nuclei, bear minus-end-directed microtubule motors.

100 lasmic dynein is responsible for most of the minus-end-directed microtubule traffic within cells.

101 tin, a complex that functions with dynein in minus-end-directed microtubule transport.

102 termittent movement involving both plus- and minus-end-directed microtubule-based motilities in the p

103 is a molecular motor that drives nearly all minus-end-directed microtubule-based transport in human

104 ctin are regulators of cytoplasmic dynein, a minus end-directed, microtubule (MT)-based motor require

105 abeling revealed the attachment of dynein, a minus end-directed, microtubule-dependent motor, to the

106 y deletions in any or all of this organism's minus end-directed, microtubule-dependent motors: two re

107 x that is required for cytoplasmic dynein, a minus-end-directed, microtubule-associated motor, to eff

108 r cytoplasmic dynein is responsible for most minus-end-directed, microtubule-based transport in eukar

109 nsisting of the plus-end tracker EB1 and the minus-end-directed molecular motor Kinesin-14 is suffici

110 ingle microtubules, but reverts to canonical minus end-directed motility when anchored on the surface

111 rked MTs showed approximately equal plus and minus end-directed motility, immunofluorescence microsco

112 t approximately equal fractions of plus- and minus-end-directed motility along microtubules.

113 whether the mechanism of the unconventional minus-end-directed motility differs fundamentally from t

114 In contrast, the inhibition of Kinesin-14 minus-end-directed motility led to extended tip interact

115 ing of the structural changes underlying the minus-end-directed motility of Kinesin-14 motors (such a

116 y-conserved catalytic core, is essential for minus-end-directed motility, as mutagenesis of these nec

117 ytoplasmic dynein, the 1.2 MDa motor driving minus-end-directed motility, has been reported to move p

118 In three cases, we observed minus-end-directed motility.

119 e (MT) motor protein responsible for most MT minus-end-directed motility.

120 is controlling the switch between plus- and minus-end-directed motility.

121 Kip2, helping it overcome dynein's intrinsic minus-end-directed motility.

122 fferent membranes promotes their microtubule minus-end-directed motility.

123 on the balance between plus-end directed and minus-end directed motion.

124 ndrites indicate that models suggesting that minus end-directed motor activity is sufficient for dend

125 Cytoplasmic dynein is a large minus end-directed motor complex with multiple functions

126 r to translocation of the MTOC, in which the minus end-directed motor cytoplasmic dynein, localized a

127 Cell biologists have long speculated that a minus end-directed motor localized at kinetochores contr

128 Cytoplasmic dynein 1 (dynein) is the primary minus end-directed motor protein in most eukaryotic cell

129 mobilized on microtubules by the activity of minus end-directed motor proteins that interact either d

130 ted with centrosomal microtubules (C-MTs) by minus end-directed motor proteins.

131 nein-1 (dynein) is a microtubule-associated, minus end-directed motor that traffics hundreds of diffe

132 plus end-directed motor, kinesin-II, and the minus end-directed motor, cytoplasmic dynein in highly p

133 at links cargos of aggregated protein to the minus end-directed motor, cytoplasmic dynein.

134 lasmic Dynein 1, or Dynein, is a microtubule minus end-directed motor.

135 Cytoplasmic dynein is the major microtubule minus end-directed motor.

136 e mitotic functions of the major microtubule minus-end directed motor cytoplasmic dynein 1.

137 Crosstalk between the minus-end directed motor dynein and kinetochore-microtub

138 We find that while the minus-end directed motor Dynein cooperates with Dynactin

139 Drosophila Ncd, the other well characterized minus-end directed motor that is a homodimer, Kar3 is a

140 ve studied the shape of myosin VI, the actin minus-end directed motor, by negative stain and metal sh

141 but not KIF13B undergoes a tug-of-war with a minus-end directed motor.

142 formation of aggresomes, suggesting that the minus-end-directed motor activities of cytoplasmic dynei

143 es, where it then organizes microtubules via minus-end-directed motor activity.

144 along microtubules by using the microtubule minus-end-directed motor complex of dynein/dynactin.

145 dynein/dynactin, a multi-subunit microtubule minus-end-directed motor complex, and NuMA, a microtubul

146 Our data reveal the novel finding that a minus-end-directed motor contributes to the organization

147 Instead, we found that mutations in the minus-end-directed motor cytoplasmic dynein, completely

148 While it is clear that the minus-end-directed motor dynein is required for this pro

149 Inhibitory antibodies indicated that the minus-end-directed motor dynein is required to prevent p

150 the transport of a subset of cargoes by the minus-end-directed motor dynein, although the full exten

151 in that mediates linkage of cargoes with the minus-end-directed motor dynein.

152 Cytoplasmic dynein is the microtubule minus-end-directed motor for the retrograde axonal trans

153 cortex required Bud6p, Bim1p, and dynein, a minus-end-directed motor helping tether the receding plu

154 involved in organelle transport) and ncd (a minus-end-directed motor involved in chromosome segregat

155 Linking of CAMSAP2 to a minus-end-directed motor leads to the formation of an MT

156 ge, is transported to the nucleus via the MT minus-end-directed motor protein dynein.

157 a wide range of cellular functions for this minus-end-directed motor protein.

158 Kar3 is the minus-end-directed motor protein; Cik1 binds to Kar3 and

159 g microtubules, driven by teams of plus- and minus-end-directed motor proteins.

160 Cytoplasmic dynein is a microtubule minus-end-directed motor that is thought to power the tr

161 tor, the clustering of their minus ends by a minus-end-directed motor, and the loss of MTs by dynamic

162 mic dynein complex is an active, microtubule minus-end-directed motor, as expected.

163 motors containing the stalk and neck of the minus-end-directed motor, Ncd, fused to the motor domain

164 ative actions of the bipolar kinesin-5 and a minus-end-directed motor, which then pulls the sliding M

165 a gal suggests that the head of Nod may be a minus-end-directed motor.

166 d simulations, we propose a model on how two minus end-directed motors cooperate to ensure spindle po

167 ac-man model, in which 1) kinetochore-based, minus end-directed motors generate poleward forces for a

168 Likewise, minus end-directed motors may move cargoes toward anteri

169 ng of nuclear congression and identified two minus end-directed motors operating in parallel in this

170 plored how the balance between plus end- and minus end-directed motors, as well as the influence of m

171 Here, we have characterized the roles of two minus end-directed motors, dynein and Ncd, in such proce

172 They implicate cortical domains where minus end-directed motors, such as dynein, are activated

173 on antagonistic activities of plus end- and minus end-directed motors.

174 T, the human homologue of the KAR3 family of minus end-directed motors.

175 ng, suggesting that they were transported by minus end-directed motors.

176 by the opposing activities of plus end- and minus end-directed motors.

177 ongression depends on the cooperation of two minus end-directed motors.

178 eraction of microtubules with both plus- and minus-end directed motors bound to a fluid membrane.

179 es (MTs) are substrates upon which plus- and minus-end directed motors control the directional moveme

180 Kinesin-14 family proteins are minus-end directed motors that cross-link microtubules a

181 act at microtubule tips synergistically with minus-end directed motors to produce a system that can g

182 bidirectionally, employing both plus-end and minus-end directed motors.

183 However, in the SAC model, minus-end-directed motors bind the minus ends of MTs as

184 -end binding proteins, suggesting that these minus-end-directed motors could interact with growing mi

185 se mitotic spindles with inactive kinesin-14 minus-end-directed motors often have shorter spindle len

186 -directed motors are balanced by forces from minus-end-directed motors that pull spindle poles togeth

187 ytoskeleton has pointed instead to roles for minus-end-directed motors.

188 be created by complexes combining plus- and minus-end-directed motors.

189 rucial role in the coordination of plus- and minus-end-directed motors.

190 hat bound to the microtubule (MT) engaged in minus end-directed movement, and that too for a short du

191 d for a single step and force generation for minus-end directed movement generated by this non-proces

192 However, the functional necessity of minus-end-directed movement along actin is unclear as th

193 ergo either diffusive, or highly processive, minus end-directed movements along microtubules.

194 bule tracks proceeds in a series of plus and minus end-directed movements that are facilitated by kin

195 owed a specific decrease in the frequency of minus end-directed movements.

196 or motor protein responsible for microtubule minus-end-directed movements in most eukaryotic cells.

197 ated with RNA localization signals mediating minus end-directed mRNA transport during Drosophila deve

198 organization that requires the activity of a minus-end-directed MT motor, cytoplasmic dynein.

199 movements), which are commonly attributed to minus end-directed, MT-dependent motors.

200 we present cryo-EM structures of the unique minus-end directed myosin VI motor domain in rigor (4.6

201 Myosin VI (Myo6) is the only minus-end directed nanomotor on actin, allowing it to un

202 lus end-directed kinesin heavy chain and the minus end-directed Ncd.

203 members; MT plus-end-directed kinesin and MT minus-end-directed non claret disjunctional (ncd).

204 ried out with monomeric motor domains of the minus-end-directed nonclaret disjunctional (Ncd) and Kar

205 the dynein heavy chain dramatically inhibits minus end-directed organelle transport and that purified

206 tively regulate interphase processes such as minus-end-directed organelle trafficking.

207 d dynactin in a search-capture mechanism for minus-end-directed organelles.

208 ecipitously shortly before transport becomes minus-end directed (phase III).

209 the rapid activation of dynein-dependent MT minus-end-directed pigment granule movement in Xenopus m

210 Furthermore, replacing dynein with a minus-end directed plant kinesin linked to the actin cor

211 be consistent with the previously discovered minus-end directed power stroke of ncd, occurring with A

212 jacent microtubule protofilaments and uses a minus-end-directed powerstroke to drive ATP-dependent mo

213 een found to naturally exhibit long-distance minus-end-directed processive motility on single microtu

214 terminal microtubule-binding tail to achieve minus-end-directed processivity on single microtubules o

215 d APC-RNPs track shrinking MTs, producing MT minus-end-directed RNA motility due to the high dwell ti

216 imulates dynein motor activity and increases minus-end-directed runs of pigment granules.

217 ing the frequency, velocity, and duration of minus-end-directed runs.

218 We propose that the minus end-directed stepping action of Cut7 is selectivel

219 r domains with their neighbors inhibit their minus end-directed stepping action, but not their plus e

220 Furthermore, generic minus-end-directed tension forces, generated by tetherin

221 r Klp2, a kinesin-14, converts Cut7 from net minus end-directed to net plus end-directed stepping.

222 h greater crowding converting the motor from minus end-directed to plus end-directed stepping.

223 The major function of dynactin is minus end-directed transport along microtubules in a com

224 nucleus occurs through a well characterized minus end-directed transport along microtubules.

225 egation signals in melanophores stimulate MT minus end-directed transport by the increasing number of

226 on the reduction of dimensionality to enable minus end-directed transport in cellulo and that complex

227 This minus end-directed transport is largely unaffected by ge

228 bule-dependent motors, we establish that the minus end-directed transport of SVs requires the dynein/

229 decreases dynein binding to APC to stimulate minus end-directed transport, which could modulate endoc

230 capture membrane organelles destined for MT minus end-directed transport.

231 ificantly contribute to the efficiency of MT minus-end directed transport of membrane organelles.

232 otor that utilizes ATP hydrolysis to conduct minus-end directed transport of various organelles.

233 dynein is the primary motor for microtubule minus-end-directed transport and is indispensable to euk

234 principal microtubule organizing center) by minus-end-directed transport and then switch polarity an

235 to threefold and at the same time decreased minus-end-directed transport of mitochondria along axona

236 ssembly and reassembly of MTs and concurrent minus-end-directed transport of pigment granules bearing

237 ndicating that this interaction mediates the minus-end-directed transport of the viral genome along m

238 ndria by disturbing the balance of plus- and minus-end-directed transport rather than directly affect

239 ons in Myo6, the gene encoding the (F-actin) minus end-directed unconventional myosin, myosin VI, cau

240 Cytoplasmic dynein drives the majority of minus end-directed vesicular and organelle motility in t