ライフサイエンスコーパス: misincorporate

1 ed low fidelity on template T, preferring to misincorporate a G instead of an A.

2 m, we demonstrate that RNA polymerase II can misincorporate a nucleotide and carry out template-depen

3 When bypass did occur, pol gamma misincorporated a guanine residue opposite the 3'-thymin

4 e C, and MutY DNA glycosylase, which removes misincorporated A opposite 8-oxoG.

5 The adenine glycosylase MutY, which removes misincorporated A residues from OG:A mismatches, is unab

6 -glycosylase and increased the efficiency of misincorporating A opposite the lesion by DNA polymerase

7 lta and yeast Pol eta both bypass 8-oxoG and misincorporate adenine during bypass.

8 ase) activity coupled with MutY's removal of misincorporated adenine bases was sought using both qual

9 the prevention of DNA mutations by removing misincorporated adenine residues from 7, 8-dihydro-8-oxo

10 of mutations associated with OG by removing misincorporated adenine residues from OG:A mismatches.

11 cision repair (BER) glycosylase that removes misincorporated adenine residues from OG:A mispairs, as

12 deoxyguanosine (OG) in DNA by the removal of misincorporated adenine residues in OG:A mispairs.

13 nine DNA glycosylase, can effectively remove misincorporated adenines opposite template G or 8-oxoG b

14 other Pols in that whereas purified Poltheta misincorporates an A opposite 1,N (6)-ethenodeoxyadenosi

15 r T was 5' to the lesion; dAMP and dTMP were misincorporated at a frequency of 2-4%.

16 It has been suggested that BMAA is misincorporated at serine codons during protein synthesi

17 ability of extension after misalignment of a misincorporated base on the next (complementary) templat

18 elity; seven unique mutants that efficiently misincorporate bases and/or extend mismatched bases were

19 *); while mainly error-free, the identity of misincorporated bases is influenced by local sequence co

20 has a relatively low ability to extend from misincorporated bases, accounting for the low level of o

21 poration, and 3) proofreading by excision of misincorporated bases.

22 separate 3'-5' exonuclease activity to edit misincorporated bases.

23 The paradox of misincorporating both a smaller (glycine) and a larger (

24 Second, free 8-oxo-dGTP can be misincorporated by DNA polymerases into DNA opposite tem

25 xcises nucleotides including antiviral drugs misincorporated by the low-fidelity viral RNA-dependent

26 by causing premature chain termination when misincorporated by the mitochondrial RNA polymerase (POL

27 chain-terminating antiviral nucleotide when misincorporated by viral RNA-dependent RNA polymerases.

28 y, epigenetic inheritance, rapid turnover of misincorporated CenH3, and transcriptional quiescence of

29 Backtracked/misincorporated complexes can be resolved via hydrolysis

30 ervation implies that physical resolution of misincorporated complexes may be the main function of th

31 TL by Gre factor occurs only in backtracked/misincorporated complexes, and not in correctly elongati

32 ells can be explained by the accumulation of misincorporated complexes, rather than mistakes in matur

33 s lacking proofreading factors are, in fact, misincorporated complexes.

34 e mass spectrometry, we find accumulation of misincorporated cytoplasmic PG precursors in the absence

35 8-oxodG, Fapy.dG instructs DNA polymerase to misincorporate dA opposite it in vitro.

36 The catalytic subunit alone primarily misincorporated dAMP and dGMP opposite the BaP DE-dG add

37 d K289M protein revealed that it was able to misincorporate dCTP opposite template C and dGTP opposit

38 , encodes the DNA polymerase Pol iota, which misincorporates deoxynucleotides at a high rate.

39 tic colon cancer variant of pol beta, K289M, misincorporates deoxynucleotides at significantly increa

40 ate kinetic analyses and find that hPoltheta misincorporates deoxynucleotides with a frequency of abo

41 on indicate that pol eta has a low fidelity, misincorporating deoxynucleotides with a frequency of ab

42 Using this platform, we found misincorporated dNs occurring at 1 per 10(3) to 10(5) ri

43 ynthesizes DNA with extremely high fidelity, misincorporating dTMP, dAMP, and dGMP opposite a templat

44 REV1 misincorporated dTTP and dGTP with much lower frequencie

45 previously established that both polymerases misincorporated dTTP with high frequency across from cis

46 l A-family polymerases, is uniquely prone to misincorporating dTTP opposite template G in a highly se

47 nown to be able to hydrolyze ribonucleotides misincorporated during genomic replication.

48 prevalent oxidized nucleotides, which can be misincorporated during replication, leading to mutations

49 ur results reveal that, in addition to being misincorporated during translation, BMAA may be able to

50 that a coupled ribosome efficiently destroys misincorporated ECs that can cause conflicts with replic

51 d nontargeted proteomics confirm that Aze is misincorporated for Pro during protein biosynthesis, spe

52 4 glycosylase indicated that there was more misincorporated FU:Gua in the DNA of MMR-deficient HCT11

53 all four diol epoxide adducts, preferring to misincorporate G and A at frequencies 3- to more than 50

54 Rev1 misincorporates G, A, and T residues opposite template G

55 h error-free and mutant transcripts with AMP misincorporated immediately downstream from the lesion.

56 It enhances both cleavage of ribonucleotides misincorporated in DNA duplexes, and the comprehensive h

57 s, we have engineered C. albicans strains to misincorporate increasing levels of Leu at protein CUG s

58 ion of transfected luciferase RNA containing misincorporated inosine in both wild-type and Itpa-null

59 iron does not normally bind SOD2, iron will misincorporate into Saccharomyces cerevisiae Sod2p when

60 om uracil, the latter accumulating and being misincorporated into DNA during folate depletion, the DN

61 Ribonucleotides are frequently misincorporated into DNA during replication, and they ar

62 , RNase H2 can remove single ribonucleotides misincorporated into DNA during replication.

63 g (MYH) is responsible for removing adenines misincorporated into DNA opposite guanine or 7,8-dihydro

64 f oxidative modification of dGTP, thatcan be misincorporated into DNA, causing AT-->CG mutations.

65 NA repair nuclease whose substrate is uracil misincorporated into DNA.

66 nome stability as it removes ribonucleotides misincorporated into genomic DNA by replicative polymera

67 Here, we show that fluorothreonine can be misincorporated into protein in place of the proteinogen

68 Ribonucleotides are misincorporated into replicating DNA due to the similari

69 gation as well as removal of incorrect bases misincorporated into RNA.

70 chromatography have suggested that uracil is misincorporated into the DNA of patients with megaloblas

71 eins contained sub-stoichiometric amounts of misincorporated lead and uranium.

72 In the absence of PCNA, pol delta misincorporates less than one nucleotide for every 100,0

73 ll of the analogues, even though this enzyme misincorporates natural NTPs at frequencies as high as 1

74 DNA polymerase makes errors by misincorporating natural DNA bases and base analogs.

75 Primase did not efficiently misincorporate NTPs during the initiation reaction (i.e.

76 lymerization revealed that human primase can misincorporate NTPs via both template misreading and a p

77 It misincorporated NTPs at frequencies as high as 1 in 7, a

78 Even though primase misincorporates NTPs at a relatively high frequency, thi

79 at the target was determined to be about one misincorporated nucleotide per 1900 repaired uracil resi

80 is stimulation is much higher in the case of misincorporated nucleotide.

81 ditionally, we found that herpes primase can misincorporate nucleotides both by misreading the templa

82 A polymerases are high fidelity enzymes that misincorporate nucleotides into nascent DNA with a frequ

83 an Poleta are low-fidelity enzymes, and they misincorporate nucleotides with a frequency of 10(-2)-10

84 es show that on undamaged DNA, both proteins misincorporate nucleotides with frequencies of approxima

85 t suppress genetic instability by correcting misincorporated nucleotides and insertion/deletion mispa

86 on by its polymerase activity and removal of misincorporated nucleotides by its exonuclease activity.

87 tivity of replicative DNA polymerase excises misincorporated nucleotides during DNA synthesis, but th

88 l II relies on its residues to recognize the misincorporated nucleotides during the backtracking proc

89 exonuclease activity that is used to remove misincorporated nucleotides from the nascent DNA (proofr

90 e non-Watson-Crick base pairs and excise the misincorporated nucleotides from the nascent DNA strand,

91 ease activity, pol II quantitatively removed misincorporated nucleotides from the nascent transcript

92 e context and that the distribution of these misincorporated nucleotides is not localized to any spec

93 ta was relatively accurate, the human enzyme misincorporated nucleotides opposite the lesion with fre

94 ation, the hydrolytic reaction stimulated by misincorporated nucleotides proofreads most of the misin

95 established MMR functions include correcting misincorporated nucleotides that have escaped the proofr

96 ch repair (MMR) is responsible for detecting misincorporated nucleotides, resulting in excision repai

97 oofreading exonuclease activity for removing misincorporated nucleotides.

98 lity, Pol II backtracks and then cleaves the misincorporated nucleotides.

99 d are featured with 5'-monophosphates and 3'-misincorporated nucleotides.

100 nt 3'-->5' exonuclease activity that excises misincorporated nucleotides.

101 e DNA but also have the ability to proofread misincorporated nucleotides.

102 ll four nondamaged template bases, hPol(eta) misincorporates nucleotides with a frequency of approxim

103 The I260Q hinge variant of polymerase beta misincorporates nucleotides with a significantly higher

104 i strain lacking IleRS post-transfer editing misincorporated Nva and Val in the proteome to a similar

105 g (MYH) is responsible for removing adenines misincorporated on a template DNA strand containing G or

106 tion fidelity by removing adenines that were misincorporated opposite 7,8-dihydro-8-oxo-deoxyguanines

107 on by recognition and removal of adenine (A) misincorporated opposite 8-oxo-7,8-dihydroguanine (OG).

108 oving 8-oxoG directly or by removing adenine misincorporated opposite 8-oxoG or both.

109 tY homologue (MYH or MUTYH) removes adenines misincorporated opposite 8-oxoguanine as part of the bas

110 minating 3' to the adduct site contained AMP misincorporated opposite dC.

111 g (MYH) is responsible for removing adenines misincorporated opposite DNA strands containing guanine

112 osylase MutY homolog (Myh1) excises adenines misincorporated opposite guanines or 7,8-dihydro-8-oxo-g

113 OG-associated mutations by removing adenines misincorporated opposite OG lesions during DNA replicati

114 In addition, the dATP can be misincorporated opposite the adduct.

115 n nascent transcripts, where adenosines were misincorporated opposite the lesions and their adjacent

116 MutY glycosylase excises adenines misincorporated opposite the oxidatively damaged lesion,

117 w that its mutator phenotype is specific for misincorporating opposite template A up to 6-fold more t

118 t group of base-excision enzymes that remove misincorporated or cytosine-derived uracil from nascent

119 ror correction involves the translocation of misincorporated products from the synthetic to the editi

120 mately 2-fold lower rates of excision of the misincorporated purine nucleotides opposite gamma-HOPdG

121 kinetic model in which A(anti)(+)*G(syn) is misincorporated quantitatively predicted the dA*dGTP mis

122 The transcripts containing misincorporated residues can be cleaved by the very slow

123 Replicative DNA polymerases misincorporate ribonucleoside triphosphates (rNTPs) into

124 aseHII) is the principal enzyme that removes misincorporated ribonucleoside monophosphates (rNMPs) fr

125 ciently remove a tyrosine linked to a single misincorporated ribonucleotide or to polyribonucleotides

126 All DNA polymerases misincorporate ribonucleotides despite their preference

127 n mutagenic profile of DnaE, we propose that misincorporated ribonucleotides are removed by NER follo

128 Therefore, misincorporated ribonucleotides are targeted by the cell

129 Misincorporated ribonucleotides in DNA will cause DNA ba

130 roposed role of this enzyme in the repair of misincorporated ribonucleotides rather than (or in addit

131 relative contribution of DNA:RNA hybrids and misincorporated ribonucleotides to chromosome instabilit

132 cies for initiating and completing repair of misincorporated ribonucleotides, archaea such as Thermoc

133 rade transcription intermediates, and repair misincorporated ribonucleotides, in preventing genome in

134 iphosphate pools result in approximately two misincorporated rNMPs/kb of DNA.

135 incorporated template mutations, as well as misincorporated sequences in telomeres, a phenotype not

136 hts the exceptional ability of the enzyme to misincorporate T opposite C and T in sequence contexts c

137 e, Pol theta can efficiently extend from the misincorporated T:G or T:T mismatches, yet with a prefer

138 We find that D246V misincorporates T opposite template bases G and C.

139 emplate C with extraordinarily low fidelity, misincorporating T, A, and C with unprecedented frequenc

140 Primase readily misincorporates the natural NTPs and will generate a wid

141 Herpes simplex virus-1 primase misincorporates the natural NTPs at frequencies of aroun

142 that DNA dynamics determine the frequency of misincorporating the A*G mismatch, and altered dynamics

143 the TL is required to transfer the 3' end of misincorporated transcript from cleavage-inefficient 'mi

144 This enhanced preferential cleavage of misincorporated transcripts suggests an important role f

145 liate Paramecium tetraurelia stereotypically misincorporates TTP at telomerase RNA templating nucleot

146 y of a recoded beta-galactosidase variant by misincorporating tyrosine in place of phenylalanine.

147 wn about the role of base excision repair of misincorporated uracil in neuronal survival.

148 or PCNA-dependent postreplicative removal of misincorporated uracil.

149 eavage of the host DNA, which possesses more misincorporated uracils than that of the phage.

150 the removal of adenines or 2-hydroxyadenines misincorporated with template guanines or 7,8-dihydro-8-