ライフサイエンスコーパス: mitoplast

1 present in highly purified mitochondria and mitoplasts.

2 mbrane space and can be observed in isolated mitoplasts.

3 proteolysis in both intact mitochondria and mitoplasts.

4 ction in UCP1 proton current in PE-deficient mitoplasts.

5 ysical properties by directly patch clamping mitoplasts.

6 ained by a patch-clamp approach in rat liver mitoplasts.

7 d quantified the amount of tagged subunit in mitoplasts and holo-CI by non-native and native PAGE, re

8 loop connecting helices 2 and 3 of QPs3, in mitoplasts and submitochondrial particles.

9 ptibility of a C-terminal Myc epitope tag in mitoplasts but not intact mitochondria.

10 Our results indicate that the mitoplasts contain just 15-19% of the outer membrane mar

11 ll as the inner CPT-II, was localized in the mitoplast fraction.

12 itochondria and mouse liver mitochondria and mitoplast fractions derived from these preparations poss

13 Brain mitoplasts from 10-day BNF-treated rats and also purifie

14 Mitoplasts from p(0) mitochondria display PTP currents i

15 10(-10) and 10(-8) M, respectively, whereas mitoplasts had lost the high affinity binding.

16 le mitochondrial extracts were obtained, and mitoplasting helped distinguish copper species in the in

17 As tested with mitoplasts, holocytochromes c from a range of species we

18 detected by direct patch clamp recording of mitoplasts, increased O(2) consumption and decreased rea

19 ious subfractions of rat liver mitochondria (mitoplast, inner membrane, intermembrane, and matrix) as

20 ondrial membrane potential but was absent in mitoplasts lacking an outer mitochondrial membrane.

21 was susceptible to proteinase K digestion in mitoplasts (mitochondria with a disrupted outer membrane

22 und that MICU1 occlusion was not detected in mitoplasts not because MICU1 cannot block, but because M

23 Mitoplast patch clamping studies showed that mPTP channe

24 In addition, the MCU mitoplast patch-clamp current (IMCU) was largely unaffec

25 However, mitoplast patch-clamp experiments argue that MICU1 does

26 d channel activity in mitochondria using the mitoplast patch-clamp technique.

27 different rescues, and loss of MICU1 during mitoplast preparation, that together might have obscured

28 ent fatty acid oxidation was retained in the mitoplasts, showing that they were physiologically intac

29 Patch clamp recordings in isolated mitoplasts suggest insertion into the inner mitochondria

30 e possibility that most of the mitochondrial/mitoplast TGase activity is due to TGase 2, the TGase is

31 The identity of the mitochondrial/mitoplast TGase(s) is not yet known.

32 tely 0.6 mM) is the same in mitochondria and mitoplasts, the same as that of AMPPNP, and is not alter

33 e c from cardiolipin-lacking mitochondria or mitoplasts under our standard experimental conditions wa

34 nel opening in intact cells or patch-clamped mitoplasts unless atractylate is added.

35 initively identify the channel, we use whole-mitoplast voltage-clamping, the technique that originall

36 n addition, the MCC activity of mouse kidney mitoplasts was unaffected by carboxyatractyloside, a kno

37 These mitoplasts were shown by electron microscopy to have the

38 IV activity in isolated mitochondria and in mitoplasts, whereas other ceramide species, sphingomyeli

39 e distinguished by chymotrypsin treatment of mitoplasts, which eliminates the action of Mg2+ but does

40 ryanodine receptor channel activity in heart mitoplasts with biophysical and pharmacological properti

41 en (Km approximately 1 nM), as were those of mitoplasts with broken outer membranes (Km approximately

42 upon short incubation of isolated fibroblast mitoplasts with dibutyryl cAMP and ATP, which also promo

43 Pretreatment of isolated mitoplasts with the anti-TbTim17 antibody inhibited impo