ライフサイエンスコーパス: molecular crowding

1 tracting muscle than in vitro because of the molecular crowding.

2 ation by ATP is facilitated by conditions of molecular crowding.

3 y of DNAs >250 bp in cytoplasm is because of molecular crowding.

4 ther macromolecules, creating a condition of molecular crowding.

5 spectives to propose a more holistic view of molecular crowding.

6 ns, stabilizing intermediates, and providing molecular crowding.

7 ation experiments reveal a prominent role of molecular crowding.

8 m expanded to compacted state upon increased molecular crowding.

9 ts surroundings via binding interactions and molecular crowding.

10 the cellulose-pectin contacts are not due to molecular crowding.

11 ed, indicating that CCVs sort their cargo by molecular crowding.

12 oss the cell cytoplasm is slowed down due to molecular crowding.

13 also promoted by high osmotic potentials or molecular crowding.

14 ffects on macromolecular assembly processes: molecular crowding.

15 cules can fine-tune gene circuit response to molecular crowding.

16 used model system for studying intracellular molecular crowding.

17 complexes with other sticky proteins due to molecular crowding.

18 eractions that are effectively equivalent to molecular crowding.

19 essive on artificial actin bundles formed by molecular crowding.

20 residues in the ER enhances even further the molecular crowding, accelerating protein denaturation.

21 This article shows that molecular crowding affects differently the transit of va

22 how perturbed ionic balance, in addition to molecular crowding, affects membrane trafficking.

23 ted sample of ubiquitin in the commonly used molecular crowding agent bovine serum albumin.

24 nded DNA template in the presence of a macro-molecular crowding agent, and that this coupled "two-pro

25 presence of polyethylene glycol, added as a molecular crowding agent.

26 leration of facilitated diffusion of DBPs by molecular crowding agents and crowder-concentration-depe

27 turants, salts, viscosity enhancers or macro-molecular crowding agents has an impact on the physical

28 However, the effect that molecular crowding agents have on the folding and cataly

29 rcoiling activity of Mtb DNA gyrase by these molecular crowding agents likely results from a decrease

30 cleavage assays to determine the effect of a molecular crowding agents on the folding and catalysis o

31 ar that they can be independently altered by molecular crowding agents or via external fields.

32 lar sandwiched structures in the presence of molecular crowding agents.

33 ctures normally seen only in the presence of molecular crowding agents.

34 o of cell mass to volume, is an indicator of molecular crowding and a fundamental determinant of cell

35 environment effects in RNA folding, such as molecular crowding and cotranscriptional kinetic effects

36 To explore the effects of molecular crowding and excluded volume upon protein stab

37 owever, the relationship between cytoplasmic molecular crowding and morphological growth states is un

38 terial in which the effects of biochemistry, molecular crowding and physical forces are complex and i

39 used to address the effect of intracellular molecular crowding and related hydration on a model telo

40 ory of glass formation based on the ideas of molecular crowding and resultant string-like cooperative

41 Their biomimetic properties, such as molecular crowding and selective partitioning, make them

42 In this work, the effects of molecular crowding and several physiologically important

43 ns for this behavior are: immobile barriers, molecular crowding, and binding interactions with immobi

44 he vectorial nature and rate of translation, molecular crowding, and cellular biosynthetic machinery.

45 ctors including changes in motor density and molecular crowding, and in multimotor motility assays.

46 the solubility of sickle hemoglobin, due to molecular crowding, and provides a useful ranking tool f

47 The effects of CA concentration, molecular crowding, and the conformational variability o

48 amp motions are increased in the presence of molecular crowding, are unchanged in the presence of ele

49 We discuss molecular crowding as an important driver of condensatio

50 nce of polyethylene glycol to rule out inert molecular crowding as the driving force for the protein

51 So far unexplored, molecular crowding at the surface of synthetic cells eme

52 t average height increases with cell surface molecular crowding but decreases with solution crowding

53 itory effect is the predicted consequence of molecular crowding by high concentrations of unrelated s

54 ts of thermodynamic nonideality arising from molecular crowding by trimethylamine N-oxide on the self

55 lar complexes, such as ribosomes, and hence, molecular crowding can impact a range of cellular proces

56 present model for polymerization, including molecular crowding, can accurately predict the rates of

57 In cells, molecular crowding caused by the presence of organelles,

58 nstants can be 13- to 163-fold higher in the molecular crowding cellular environment.

59 Here, we report that molecular crowding comparable to that in the cell compen

60 Furthermore, by manipulation of molecular crowding conditions (entropic forces), we were

61 We determined that molecular crowding conditions and cations, such as Na(+)

62 gher than 37 degrees C, especially under the molecular crowding conditions and in the presence of K(+

63 ges occurring under physiologically relevant molecular crowding conditions in ultrathin biosensor lay

64 It is essential to study RNA under molecular crowding conditions to better predict secondar

65 However, the molecular crowding conditions within cells diminish the

66 nce of these DNA GQs can be dependent on the molecular crowding conditions.

67 uadruplex stabilizers even under challenging molecular crowding conditions.

68 edominant form of the holoenzyme, even under molecular crowding conditions.

69 ion osmotic pressure, thus mimicking in vivo molecular crowding conditions.

70 delay suggests that, although volume-induced molecular crowding contributes to trafficking defects, i

71 ferative state, suggesting that the level of molecular crowding decreases and becomes more regulated

72 hich could not be rationalized by effects of molecular crowding, dehydration, or temperature.

73 tanding of the delicate balance that governs molecular crowding driven by RNA and its implications fo

74 Our method reveals unexpected behaviour in molecular crowding during cell state transitions and sug

75 tracking, we observed a striking decrease in molecular crowding during filamentous growth in C. albic

76 We demonstrate that two-dimensional molecular crowding, emulated by the PEG molecules at the

77 ikely more relevant to natural systems since molecular crowding events in the cytosol can influence t

78 NMR structures solved under molecular crowding experiments correlate with the crysta

79 vious theory of overflow metabolism based on molecular crowding following the proteomic fractions for

80 This study suggests that molecular crowding forces can increase protein misfoldin

81 Indeed, increasing molecular crowding has been shown to modify the kinetics

82 The field of molecular crowding has its origins in the far 80s but be

83 we provide a physiology-focused overview of molecular crowding, highlighting contemporary advances i

84 the predominant product under conditions of molecular crowding; however, we also discovered that Rec

85 The data suggests molecular crowding impacts myosin conformation, implying

86 n vitro at physiological protein levels upon molecular crowding in buffers that resemble physiologica

87 The results suggest that molecular crowding in cellular membranes has a dramatic

88 reactions in vitro; however, the effects of molecular crowding in living cells are mostly unexplored

89 Despite the ubiquity of molecular crowding in living cells, the effects of crowd

90 ere the myosin concentration is low suggests molecular crowding in the A-band promotes occupancy of t

91 lates the molecular composition and level of molecular crowding in the ER to the kinetic rates of pro

92 localized actin polymerization and increased molecular crowding in the lamellipodium to explain how c

93 One possible factor could be molecular crowding in the neuronal cytoplasm.

94 ugh the binding of NTRs to FG Nups increases molecular crowding in the NPC transport channel, it is u

95 f entropic effects caused by confinement and molecular crowding in the spatial organization of the ch

96 g and point to solvation inhomogeneities and molecular crowding in these concentrated fluids.

97 ery low permeabilities and a small effect of molecular crowding in volumes between the barriers.

98 presence of membranes, a condition mimicking molecular crowding induced by dehydration during freezin

99 As a model for understanding how molecular crowding influences diffusion and transport of

100 ively subtle changes in CA concentration and molecular crowding influencing self-assembly and the ens

101 of proteins, tasks that are hindered by the molecular crowding inside cells, imaging distortions cha

102 rtionally to Klp9 molecules, suggesting that molecular crowding inversely correlates to cell size and

103 g the chemical underpinnings of RNA-mediated molecular crowding, investigating the role of modificati

104 Thus, molecular crowding is a fundamental physiologic paramete

105 We show that molecular crowding is a key factor in explaining the swi

106 alous diffusion in cells as a consequence of molecular crowding is not correct and that slowing of di

107 Instead, molecular crowding itself completely alters the mechanis

108 Apparently, the molecular crowding level that can inhibit the transport

109 pinging on the degree of cellular hydration, molecular crowding, magnitude of turgor, and cellular in

110 Cells are densely packed with proteins, and molecular crowding may play an important role in cellula

111 e acceleration of binding site maturation by molecular crowding may represent a general mechanism for

112 Molecular crowding modifies biochemical reaction rates a

113 In addition, we bring evidence that molecular crowding modulates competitive binding and dem

114 ent evidence underscores the significance of molecular crowding-most likely via liquid-liquid phase s

115 A combination of molecular crowding, non-ionic detergents, Mg(2+) ions, a

116 We also show that dilncRNAs drive molecular crowding of DDR proteins, such as 53BP1, into

117 This is because the molecular crowding of non-polymerizing HbF offsets subst

118 smotically induced changes primarily through molecular crowding of solutes that affect channel activi

119 cattering was used to examine the effects of molecular crowding on an intrinsically disordered protei

120 summary, the study found that the effects of molecular crowding on both conformational dynamics and c

121 s study provides insights into the effect of molecular crowding on FRET, and it offers a basis for in

122 ttractive model to investigate the effect of molecular crowding on ligand-induced protein folding, as

123 computational methods to study the impact of molecular crowding on protein-protein interactions and d

124 s been completed to determine the effects of molecular crowding on RNA duplexes of varying lengths an

125 We review the effects of molecular crowding on solute diffusion in solution and i

126 recent studies have highlighted the role of molecular crowding on the effects of hypertonicity, the

127 The effect of molecular crowding on the structure and function of Esch

128 The effects of "molecular crowding" on elementary biochemical processes

129 membrane surface binding sites, rather than molecular crowding or aggregation, which is the case for

130 Molecular crowding plays a significant role in regulatin

131 Our data is consistent with the idea that molecular crowding plays an important role in the stabil

132 s study highlights the often overlooked role molecular crowding plays in regulating molecular structu

133 ical constraints and is activated in vivo by molecular crowding rather than changes in cell size.

134 vironmental factors, such as pH, salt, other molecular crowding reagents, and specifically designed "

135 n intracellular hydration, concentration and molecular crowding, rendering HOPS one of many related p

136 These results suggest that molecular crowding represents a bound on the achievable

137 nation for significant sequestration even if molecular crowding results in a very low critical concen

138 rapidly responds to changes in hydration and molecular crowding, revealing an unexpected mode of glob

139 NKCC1 activation by the molecular crowding sensing WNK kinases is critical if ce

140 ts on the single-molecule kinetics of solute molecular crowding, specifically focusing on GAAA tetral

141 We have shown previously that molecular crowding stabilizes folded RNA structures.

142 system causes spatio-temporal differences in molecular crowding states that are sufficient to drive a

143 decreases with increasing GM1 content due to molecular crowding stemming from GM1 clustering.

144 Estimates of excluded volume effects due to molecular crowding suggest the oligomerization equilibri

145 pseudostable DNA hairpin highly sensitive to molecular crowding that displayed a shift in melting tem

146 The interactions may involve molecular crowding that favors coalescence of the hetero

147 In the case of molecular crowding, translational mobility was assessed

148 anges, possibly reflective of an increase in molecular crowding upon cell shrinkage.

149 rease in the effective membrane viscosity or molecular crowding upon membrane bending.

150 a mixing law that describes the increase of molecular crowding upon reduction of the intracellular f

151 s and in the presence of dextran that causes molecular crowding verify a strict Ca2+ requirement of t

152 lational proteins to alleviate the effect of molecular crowding, we develop a model for cell growth b

153 We therefore considered molecular crowding, where colloids with lower protein su

154 ion signal was found to be more sensitive to molecular crowding, whereas the aptamer probe signal doe

155 ase activity of Hsp90 is highly sensitive to molecular crowding, whereas the ATPase activities of Hsp

156 on in cell volume and subsequent increase in molecular crowding which severely alters the biophysical

157 Molecular crowding, which is microtubule independent, ca

158 ctility as well as water efflux that changes molecular crowding within individual cells.

159 ormational change is altered dramatically by molecular crowding within the peptide monolayer.

160 se was insufficient in this regard, and that molecular crowding within the synapse is critical to pre