ライフサイエンスコーパス: msDNA

1 msDNA is synthesized from an RNA template by the retron-

2 produces multi-copy single-stranded DNA(1) (msDNA).

3 m comprises a reverse transcriptase (RT), an msDNA, and an overcoming lysogenization defect (OLD)-fam

4 ymmetric 4:4:4 assembly of RT, Eco8-OLD, and msDNA (a hybrid of msrRNA and msdDNA).

5 nstrate interference with mismatch repair by msDNA, we show that the addition of a plasmid containing

6 e production of a small satellite DNA called msDNA.

7 ly obscure RNA-DNA satellite molecule called msDNA.

8 msDNA, the RT, and the retron are designated msDNA-Ec48, RT-Ec48, and retron-Ec48, respectively.

9 at synthesize multicopy single-stranded DNA (msDNA) and are being adapted for genome engineering.

10 that produce multicopy single-stranded DNA (msDNA) and hold promise for genome engineering.

11 Multicopy single-stranded DNA (msDNA) molecules consist of single-stranded DNA covalent

12 ucture of the multicopy single-stranded DNA (msDNA) produced by the RT.

13 e DNAs called multicopy single-stranded DNA (msDNA).

14 Previously, we showed that retrons encoding msDNAs with mismatched base pairs are mutagenic when pre

15 on using an RNA (the primer-template RNA for msDNA) as a template and the 3' end of a nick in the chr

16 A strand and five bases of the RNA strand in msDNA-Ne144.

17 tides; it is the longest RNA among the known msDNAs.

18 We also show that in mutS mutants, msDNA does not increase the frequency of either mutation

19 e decades of research on the biosynthesis of msDNA(2), the function and physiological roles of retron

20 The presence of this truncated copy of msDNA is strong evidence of retrotransposition in N. exe

21 This partial copy of msDNA is very likely a retrotransposed sequence that was

22 ructure of the msr gene, the RNA molecule of msDNA-Ec48 is predicted to be composed of 119 ribonucleo

23 lex, and becomes active upon perturbation of msDNA biosynthesis.

24 activation and highlight the central role of msDNA as a molecular switch in controlling retron-mediat

25 underscores the dynamic regulatory roles of msDNA, advancing our understanding of antiphage defense

26 equences match only part of the sequences of msDNA and may have become incorporated into the chromoso

27 he gene msr (which encodes the RNA strand of msDNA) appears to be duplicated and flanks both sides of

28 he gene msd (which encodes the DNA strand of msDNA).

29 the mutagenic and recombinogenic effects of msDNAs are due to titrating out MutS protein.

30 the mutagenic and recombinogenic effects of msDNAs.

31 The DNA moieties of msDNAs have characteristic stem-loop structures, and mos

32 The resulting products (msDNA) consist of RNA and single-stranded DNA, sharing h

33 NA of 48 nucleotides in length, the shortest msDNA thus far identified from natural sources.

34 In this study we show that such msDNAs, in a similar manner to genetic defects in mismat

35 The msDNA anchors RT and Eco8-OLD into a compact architectur

36 The msDNA was found to consist of a single-stranded DNA of 4

37 The msDNA, the RT, and the retron are designated msDNA-Ec48,

38 ase is required for binding to RcaT, and the msDNA is required for the antitoxin activity.

39 t phage-related triggers directly modify the msDNA, thereby activating RcaT and inhibiting bacterial

40 also contains several partial copies of the msDNA sequence as revealed by DNA hybridization experime

41 This msDNA is composed of a 144 base, single-stranded DNA tha

42 um is a set of repeated sequences related to msDNA.

43 omplexes comprising a reverse transcriptase, msDNA (a hybrid of msdDNA and msrRNA), and two PtuAB cop

44 is neutralized by the reverse transcriptase-msDNA antitoxin complex, and becomes active upon perturb

45 systems, which use the reverse transcriptase-msDNA complex both as an antitoxin and as a sensor of ph

46 ealed by DNA hybridization experiments using msDNA as a probe.